Daughter-Specific Transcription Factors Regulate Cell Size Control in Budding Yeast
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{"title"=>"Daughter-specific transcription factors regulate cell size control in budding yeast", "type"=>"journal", "authors"=>[{"first_name"=>"Stefano", "last_name"=>"Di Talia", "scopus_author_id"=>"18133710500"}, {"first_name"=>"Hongyin", "last_name"=>"Wang", "scopus_author_id"=>"18438778000"}, {"first_name"=>"Jan M.", "last_name"=>"Skotheim", "scopus_author_id"=>"6602331516"}, {"first_name"=>"Adam P.", "last_name"=>"Rosebrock", "scopus_author_id"=>"8720825900"}, {"first_name"=>"Bruce", "last_name"=>"Futcher", "scopus_author_id"=>"7003462669"}, {"first_name"=>"Frederick R.", "last_name"=>"Cross", "scopus_author_id"=>"35549883900"}], "year"=>2009, "source"=>"PLoS Biology", "identifiers"=>{"isbn"=>"1545-7885 (Electronic)", "pui"=>"355524103", "issn"=>"15449173", "pmid"=>"19841732", "doi"=>"10.1371/journal.pbio.1000221", "sgr"=>"70350491411", "scopus"=>"2-s2.0-70350491411"}, "id"=>"695e2c98-042e-3f77-942f-d69ea5204b71", "abstract"=>"In budding yeast, asymmetric cell division yields a larger mother and a smaller daughter cell, which transcribe different genes due to the daughter-specific transcription factors Ace2 and Ash1. Cell size control at the Start checkpoint has long been considered to be a main regulator of the length of the G1 phase of the cell cycle, resulting in longer G1 in the smaller daughter cells. Our recent data confirmed this concept using quantitative time-lapse microscopy. However, it has been proposed that daughter-specific, Ace2-dependent repression of expression of the G1 cyclin CLN3 had a dominant role in delaying daughters in G1. We wanted to reconcile these two divergent perspectives on the origin of long daughter G1 times. We quantified size control using single-cell time-lapse imaging of fluorescently labeled budding yeast, in the presence or absence of the daughter-specific transcriptional regulators Ace2 and Ash1. Ace2 and Ash1 are not required for efficient size control, but they shift the domain of efficient size control to larger cell size, thus increasing cell size requirement for Start in daughters. Microarray and chromatin immunoprecipitation experiments show that Ace2 and Ash1 are direct transcriptional regulators of the G1 cyclin gene CLN3. Quantification of cell size control in cells expressing titrated levels of Cln3 from ectopic promoters, and from cells with mutated Ace2 and Ash1 sites in the CLN3 promoter, showed that regulation of CLN3 expression by Ace2 and Ash1 can account for the differential regulation of Start in response to cell size in mothers and daughters. We show how daughter-specific transcriptional programs can interact with intrinsic cell size control to differentially regulate Start in mother and daughter cells. This work demonstrates mechanistically how asymmetric localization of cell fate determinants results in cell-type-specific regulation of the cell cycle.", "link"=>"http://www.mendeley.com/research/daughterspecific-transcription-factors-regulate-cell-size-control-budding-yeast-3", "reader_count"=>112, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>7, "Researcher"=>24, "Student > Doctoral Student"=>5, "Student > Ph. D. Student"=>45, "Student > Postgraduate"=>3, "Student > Master"=>11, "Other"=>2, "Student > Bachelor"=>10, "Professor"=>5}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>7, "Researcher"=>24, "Student > Doctoral Student"=>5, "Student > Ph. D. Student"=>45, "Student > Postgraduate"=>3, "Student > Master"=>11, "Other"=>2, "Student > Bachelor"=>10, "Professor"=>5}, "reader_count_by_subject_area"=>{"Engineering"=>4, "Biochemistry, Genetics and Molecular Biology"=>14, "Mathematics"=>1, "Agricultural and Biological Sciences"=>83, "Medicine and Dentistry"=>1, "Physics and Astronomy"=>5, "Chemistry"=>1, "Computer Science"=>3}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>4}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>5}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>83}, "Computer Science"=>{"Computer Science"=>3}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>14}, "Mathematics"=>{"Mathematics"=>1}}, "reader_count_by_country"=>{"Canada"=>1, "Argentina"=>1, "United States"=>1, "China"=>1, "Taiwan"=>1, "Denmark"=>1, "United Kingdom"=>2, "Chile"=>1, "Australia"=>1, "Germany"=>3, "India"=>1}, "group_count"=>6}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/878247"], "description"=>"<p>(A) Illustration of the separation of G1 into two intervals, T<sub>1</sub> and T<sub>2</sub>, by using Whi5-GFP. The total duration of G1 is T<sub>1</sub>+T<sub>2</sub>. (B–H) Correlation between αT<sub>1</sub> and ln(M<sub>birth</sub>) for cells grown in glucose or glycerol/ethanol. (B–C) wild-type, (D–E) <i>ace2</i>, (F–G) <i>ash1</i>, (H–I) <i>ace2 ash1</i>. Red dots, mothers; blue dots, daughters. Inset: cartoon illustrating presence of Ace2 or Ash1 in mother and daughter nuclei; black semicircle, Ace2; pink semicircle, Ash1. Gray bars indicate the region of size overlap used for the analysis presented in <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1000221#pbio-1000221-t001\" target=\"_blank\">Table 1</a>.</p>", "links"=>[], "tags"=>["ace2"], "article_id"=>548704, "categories"=>["Cell Biology", "Developmental Biology"], "users"=>["Stefano Di Talia", "Hongyin Wang", "Jan M. Skotheim", "Adam P. Rosebrock", "Bruce Futcher", "Frederick R. Cross"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1000221.g001", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Differential_regulation_of_Start_is_dependent_on_Ace2_and_Ash1_/548704", "title"=>"Differential regulation of Start is dependent on Ace2 and Ash1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-10-20 02:25:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/879329"], "description"=>"<p>The region of overlap in size at birth of mothers and daughters was evaluated for every genotype separately (see gray bars in <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1000221#pbio-1000221-g001\" target=\"_blank\">Figures 1</a>, <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1000221#pbio-1000221-g002\" target=\"_blank\">2</a>, <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1000221#pbio-1000221-g006\" target=\"_blank\">6</a>, and <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1000221#pbio-1000221-g007\" target=\"_blank\">7</a>). Data for the duration of T<sub>1</sub> in this region were divided in small bins and the daughter delays (i.e., average excess in T<sub>1</sub> for daughters over mothers) were computed for every size bin with representation of both mothers and daughters. The results were averaged across all these size bins. This definition of daughter delay is largely independent of the uneven distribution of cell size at birth in the region of overlap. The <i>p</i> value, computed by <i>t</i> test, for the hypothesis that the mutant daughter delay is the same as the wild-type daughter delay is indicated in parentheses. The statistical significance of difference in T<sub>1</sub> times between mothers and daughters in the region of size overlap is presented in <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1000221#pbio.1000221.s017\" target=\"_blank\">Table S4</a>. Asterisks indicate the dominant mutant forms. Data for the difference in T<sub>1</sub> in mother-daughter pairs are presented in <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1000221#pbio.1000221.s006\" target=\"_blank\">Figures S4</a>, <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1000221#pbio.1000221.s007\" target=\"_blank\">S5</a>, and <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1000221#pbio.1000221.s008\" target=\"_blank\">S6</a>.</p>", "links"=>[], "tags"=>["newborn", "cells"], "article_id"=>549786, "categories"=>["Cell Biology", "Developmental Biology"], "users"=>["Stefano Di Talia", "Hongyin Wang", "Jan M. Skotheim", "Adam P. Rosebrock", "Bruce Futcher", "Frederick R. Cross"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1000221.t001", "stats"=>{"downloads"=>3, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Average_daughter_delay_in_newborn_cells_of_the_same_size_/549786", "title"=>"Average daughter delay in newborn cells of the same size.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2009-10-20 02:43:06"}
  • {"files"=>["https://ndownloader.figshare.com/files/879267"], "description"=>"<p>The ratio of binding of Ace2, Ash1, and Swi5 to mutated and wild-type <i>CLN3</i> sequences in heterozygous diploids is reported (in parenthesis is the <i>p</i> value that the measured ratio is compatible with no change in binding). Binding to the <i>CLN3</i> promoter region between −1183 and −998 (ATG: +1) is significantly reduced upon mutation of the putative Ace2/Swi5 and Ash1 binding sites (see <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1000221#pbio.1000221.s019\" target=\"_blank\">Table S6</a> for details). Binding to the region from −767 to −545 is not affected.</p>", "links"=>[], "tags"=>["swi5", "binding", "promoter", "heterozygous"], "article_id"=>549722, "categories"=>["Cell Biology", "Developmental Biology"], "users"=>["Stefano Di Talia", "Hongyin Wang", "Jan M. Skotheim", "Adam P. Rosebrock", "Bruce Futcher", "Frederick R. Cross"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1000221.t002", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Ace2_Ash1_and_Swi5_Binding_to_the_CLN3_Promoter_in_Heterozygous_Diploids_/549722", "title"=>"Ace2, Ash1, and Swi5 Binding to the <i>CLN3</i> Promoter in Heterozygous Diploids.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2009-10-20 02:42:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/436224", "https://ndownloader.figshare.com/files/436253", "https://ndownloader.figshare.com/files/436280", "https://ndownloader.figshare.com/files/436332", "https://ndownloader.figshare.com/files/436400", "https://ndownloader.figshare.com/files/436468", "https://ndownloader.figshare.com/files/436555", "https://ndownloader.figshare.com/files/436668", "https://ndownloader.figshare.com/files/436761", "https://ndownloader.figshare.com/files/436839", "https://ndownloader.figshare.com/files/436921", "https://ndownloader.figshare.com/files/436968", "https://ndownloader.figshare.com/files/437021", "https://ndownloader.figshare.com/files/437088", "https://ndownloader.figshare.com/files/437189", "https://ndownloader.figshare.com/files/437231", "https://ndownloader.figshare.com/files/437266", "https://ndownloader.figshare.com/files/437309", "https://ndownloader.figshare.com/files/437355", "https://ndownloader.figshare.com/files/437405"], "description"=>"<div><p>In budding yeast, asymmetric cell division yields a larger mother and a smaller daughter cell, which transcribe different genes due to the daughter-specific transcription factors Ace2 and Ash1. Cell size control at the Start checkpoint has long been considered to be a main regulator of the length of the G1 phase of the cell cycle, resulting in longer G1 in the smaller daughter cells. Our recent data confirmed this concept using quantitative time-lapse microscopy. However, it has been proposed that daughter-specific, Ace2-dependent repression of expression of the G1 cyclin <em>CLN3</em> had a dominant role in delaying daughters in G1. We wanted to reconcile these two divergent perspectives on the origin of long daughter G1 times. We quantified size control using single-cell time-lapse imaging of fluorescently labeled budding yeast, in the presence or absence of the daughter-specific transcriptional regulators Ace2 and Ash1. Ace2 and Ash1 are not required for efficient size control, but they shift the domain of efficient size control to larger cell size, thus increasing cell size requirement for Start in daughters. Microarray and chromatin immunoprecipitation experiments show that Ace2 and Ash1 are direct transcriptional regulators of the G1 cyclin gene <em>CLN3</em>. Quantification of cell size control in cells expressing titrated levels of Cln3 from ectopic promoters, and from cells with mutated Ace2 and Ash1 sites in the <em>CLN3</em> promoter, showed that regulation of <em>CLN3</em> expression by Ace2 and Ash1 can account for the differential regulation of Start in response to cell size in mothers and daughters. We show how daughter-specific transcriptional programs can interact with intrinsic cell size control to differentially regulate Start in mother and daughter cells. This work demonstrates mechanistically how asymmetric localization of cell fate determinants results in cell-type-specific regulation of the cell cycle.</p></div>", "links"=>[], "tags"=>["daughter-specific", "transcription", "factors", "budding", "yeast"], "article_id"=>146022, "categories"=>["Cell Biology", "Developmental Biology"], "users"=>["Stefano Di Talia", "Hongyin Wang", "Jan M. Skotheim", "Adam P. Rosebrock", "Bruce Futcher", "Frederick R. Cross"], "doi"=>["https://dx.doi.org/10.1371/journal.pbio.1000221.s001", "https://dx.doi.org/10.1371/journal.pbio.1000221.s002", "https://dx.doi.org/10.1371/journal.pbio.1000221.s003", "https://dx.doi.org/10.1371/journal.pbio.1000221.s004", "https://dx.doi.org/10.1371/journal.pbio.1000221.s005", "https://dx.doi.org/10.1371/journal.pbio.1000221.s006", "https://dx.doi.org/10.1371/journal.pbio.1000221.s007", "https://dx.doi.org/10.1371/journal.pbio.1000221.s008", "https://dx.doi.org/10.1371/journal.pbio.1000221.s009", "https://dx.doi.org/10.1371/journal.pbio.1000221.s010", "https://dx.doi.org/10.1371/journal.pbio.1000221.s011", "https://dx.doi.org/10.1371/journal.pbio.1000221.s012", "https://dx.doi.org/10.1371/journal.pbio.1000221.s013", "https://dx.doi.org/10.1371/journal.pbio.1000221.s014", "https://dx.doi.org/10.1371/journal.pbio.1000221.s015", "https://dx.doi.org/10.1371/journal.pbio.1000221.s016", "https://dx.doi.org/10.1371/journal.pbio.1000221.s017", "https://dx.doi.org/10.1371/journal.pbio.1000221.s018", "https://dx.doi.org/10.1371/journal.pbio.1000221.s019", "https://dx.doi.org/10.1371/journal.pbio.1000221.s020"], "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Daughter_Specific_Transcription_Factors_Regulate_Cell_Size_Control_in_Budding_Yeast/146022", "title"=>"Daughter-Specific Transcription Factors Regulate Cell Size Control in Budding Yeast", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2009-10-20 01:40:22"}
  • {"files"=>["https://ndownloader.figshare.com/files/879038"], "description"=>"<p>Correlation between αT<sub>1</sub> and ln(M<sub>birth</sub>) for cells grown in glycerol/ethanol in mutants lacking the Ace2/Swi5 and/or Ash1 sites on the <i>CLN3</i> promoter. (A) wild-type, (B) Ace2/Swi5 sites mutated, (C) Ash1 sites mutated, (D) Ace2/Swi5 and Ash1 sites mutated. Red dots, mothers; blue dots, daughters. Gray bars indicate the region of size overlap used for the analysis presented in <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1000221#pbio-1000221-t001\" target=\"_blank\">Table 1</a>.</p>", "links"=>[], "tags"=>["ash1", "binding", "sites", "promoter", "asymmetrical"], "article_id"=>549492, "categories"=>["Cell Biology", "Developmental Biology"], "users"=>["Stefano Di Talia", "Hongyin Wang", "Jan M. Skotheim", "Adam P. Rosebrock", "Bruce Futcher", "Frederick R. Cross"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1000221.g007", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mutation_of_the_Ace2_Swi5_and_Ash1_binding_sites_on_the_CLN3_promoter_reduces_the_asymmetrical_regulation_of_Start_/549492", "title"=>"Mutation of the Ace2/Swi5 and Ash1 binding sites on the <i>CLN3</i> promoter reduces the asymmetrical regulation of Start.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-10-20 02:38:12"}
  • {"files"=>["https://ndownloader.figshare.com/files/878895"], "description"=>"<p>Experimental strategy to estimate the preferential binding of Ace2, Swi5, and Ash1 to their consensus-binding sites. Following ChIP, various regions of the <i>CLN3</i> promoter were amplified by PCR and analyzed by sequencing to obtain an estimate of the ratio of wild-type promoter sequences to mutated sequences. This ratio compared to the same ratio from PCR of genomic DNA provides an indication of the preferential binding of the factors to these sequences (<a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1000221#pbio-1000221-t002\" target=\"_blank\">Table 2</a>).</p>", "links"=>[], "tags"=>["ash1", "promoter", "reduced", "mutation", "consensus-binding"], "article_id"=>549346, "categories"=>["Cell Biology", "Developmental Biology"], "users"=>["Stefano Di Talia", "Hongyin Wang", "Jan M. Skotheim", "Adam P. Rosebrock", "Bruce Futcher", "Frederick R. Cross"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1000221.g006", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Binding_of_Ace2_Swi5_and_Ash1_to_the_CLN3_promoter_is_reduced_by_mutation_of_the_Ace2_Swi5_and_Ash1_consensus_binding_sites_/549346", "title"=>"Binding of Ace2, Swi5, and Ash1 to the <i>CLN3</i> promoter is reduced by mutation of the Ace2/Swi5 and Ash1 consensus-binding sites.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-10-20 02:35:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/878774"], "description"=>"<p><i>CLN3</i> expression: (A) <i>ACE2* ASH1*</i> versus <i>ace2 ash1</i>, (B) <i>ACE2*</i> versus <i>ace2</i>, (C) <i>ASH1*</i> versus <i>ash1</i>. The error bars were estimated from the variability in expression of the large number of genes that are not affected by Ace2 and Ash1. The expression levels of <i>CLN3</i>, as well as any other gene in the genome, were estimated by at least four measurements from four distinct probes. These measurements showed a smaller variability than the presented error bars, suggesting that the reported error bars are a conservative estimate of measurement errors. (D) Expression of a cluster of Swi5-dependent genes. Arrays from <i>GALL-CDC20</i> block release time course experiments of wild-type and <i>swi5</i> cells were hierarchically clustered. A Swi5-specific cluster is shown (see <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1000221#pbio.1000221.s020\" target=\"_blank\">Text S1</a> for a complete list of genes regulated by Swi5). (E) <i>CLN3</i> expression compared with the average expression of the remaining genes belonging to the Swi5-specific cluster (error bars indicate s.e.m.) from the dataset obtained by subtracting the <i>ACE2*</i> data from the <i>ace2</i> data. (F) <i>CLN3</i> expression compared with the average expression of the whole genome (error bars indicate s.e.m.) from the same dataset (i.e. <i>ace2</i>−<i>ACE2*</i>). ChIP analysis of the interaction between Swi5 (G), Ace2 (H), Ash1 (I), and the <i>CLN3</i> promoter. Following cross-linking and immunoprecipitation, DNA was amplified by PCR. Amplification of a region of the ORF of <i>DYN1</i> was used as negative controls, while regions of the <i>SIC1</i>, <i>CTS1</i>, and <i>HO</i> promoters were used as positive controls for Swi5, Ace2, and Ash1, respectively. All the strains were TAP-tagged (NC, negative control from an untagged strain; WCE, whole cell extract). The ChIP data were reproduced for Ace2 and Ash1. The Swi5 data are from a single experiment. (J) Correlation between αT<sub>1</sub> and ln(M<sub>birth</sub>) in daughter cells carrying different copy numbers of <i>CLN3</i>. (K) Representation of the Ace2/Swi5 and Ash1 putative binding sites on the <i>CLN3</i> promoter.</p>", "links"=>[], "tags"=>["ash1", "g1", "cyclin"], "article_id"=>549231, "categories"=>["Cell Biology", "Developmental Biology"], "users"=>["Stefano Di Talia", "Hongyin Wang", "Jan M. Skotheim", "Adam P. Rosebrock", "Bruce Futcher", "Frederick R. Cross"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1000221.g005", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Ace2_Swi5_and_Ash1_regulate_the_expression_of_the_G1_cyclin_CLN3_/549231", "title"=>"Ace2, Swi5, and Ash1 regulate the expression of the G1 cyclin <i>CLN3</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-10-20 02:33:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/878657"], "description"=>"<p>(A) Analysis of cell cycle synchronization and nuclear localization of Ace2, Swi5, and Ash1 in a <i>cdc20</i> block-release experiment. Top panel shows the percentage of mononucleate cells, large budded cells, and cells that have rebudded. The middle panel shows the levels of mitotic cyclin Clb2. The lower panel shows the dynamics of nuclear localization of fluorescently tagged Ace2, Swi5, and Ash1. (B) Expression data from the M/G1 and G1/S cell cycle regulated cluster of genes. (C) The regulation of <i>CTS1</i> (Ace2 target), <i>HO</i> (Ash1 target), and <i>SWI5</i> (Fkh1,2 Mcm1 target) expression from the microarray series, as well as data obtained by point-by-point subtraction of the arrays (<i>ACE2*</i>−<i>ace2</i>, <i>ASH1*</i>−<i>ash1</i>, <i>ACE2* ASH1*</i>−<i>ace2 ash1</i>). In these graphs, the time of anaphase, which varies slightly between experiments, was used as the zero time to make the comparisons more accurate.</p>", "links"=>[], "tags"=>["ace2", "ash1"], "article_id"=>549113, "categories"=>["Cell Biology", "Developmental Biology"], "users"=>["Stefano Di Talia", "Hongyin Wang", "Jan M. Skotheim", "Adam P. Rosebrock", "Bruce Futcher", "Frederick R. Cross"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1000221.g004", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Genome_wide_analysis_of_Ace2_and_Ash1_targets_/549113", "title"=>"Genome-wide analysis of Ace2 and Ash1 targets.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-10-20 02:31:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/879138"], "description"=>"<p>Correlation between αT<sub>1</sub> and ln(M<sub>birth</sub>) for cells grown in glucose or glycerol/ethanol. (A–B) wild-type, (C–D) <i>cln3</i>, (E) <i>cln3 6xCDC28pr-CLN3</i>, (F) <i>cln3 4xCDC28pr-CLN3</i>, (G) <i>cln3 ADH1pr-CLN3</i>. Red dots, mothers; blue dots, daughters. Gray bars indicate the region of size overlap used for the analysis presented in <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1000221#pbio-1000221-t001\" target=\"_blank\">Table 1</a>.</p>", "links"=>[], "tags"=>["symmetric", "mothers"], "article_id"=>549591, "categories"=>["Cell Biology", "Developmental Biology"], "users"=>["Stefano Di Talia", "Hongyin Wang", "Jan M. Skotheim", "Adam P. Rosebrock", "Bruce Futcher", "Frederick R. Cross"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1000221.g008", "stats"=>{"downloads"=>4, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Symmetric_regulation_of_CLN3_expression_result_in_symmetric_control_of_Start_in_mothers_and_daughters_/549591", "title"=>"Symmetric regulation of <i>CLN3</i> expression result in symmetric control of Start in mothers and daughters.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-10-20 02:39:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/879302"], "description"=>"<p>The expression of <i>CLN3</i> is cell cycle regulated with a peak in expression at M/G1 characterized by a peak to trough ratio of order 3 (see <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1000221#pbio-1000221-g005\" target=\"_blank\">Figure 5</a>) <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1000221#pbio.1000221-McInerny1\" target=\"_blank\">[44]</a>. Since the period of peak expression is brief, we consider a construct yielding ∼3 times the average expression level of endogenous Cln3 to give approximately wild-type levels of expression during the critical interval.</p>", "links"=>[], "tags"=>["cln3", "asynchronous", "populations", "expressing", "constitutive"], "article_id"=>549752, "categories"=>["Cell Biology", "Developmental Biology"], "users"=>["Stefano Di Talia", "Hongyin Wang", "Jan M. Skotheim", "Adam P. Rosebrock", "Bruce Futcher", "Frederick R. Cross"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1000221.t003", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Levels_of_Cln3_expression_and_average_cell_size_for_asynchronous_cell_populations_expressing_CLN3_from_various_constitutive_promoters_/549752", "title"=>"Levels of Cln3 expression and average cell size for asynchronous cell populations expressing <i>CLN3</i> from various constitutive promoters.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2009-10-20 02:42:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/878382"], "description"=>"<p>(A–H) Correlation between αT<sub>1</sub> and ln(M<sub>birth</sub>) for cells grown in glucose or glycerol/ethanol. (A–B) wild-type, (C–D) <i>ACE2*</i>, (E–F) <i>ASH1*</i>, (G–H) <i>ACE2* ASH1*</i>. Red dots, mothers; blue dots, daughters. Black semicircle, Ace2; pink semicircle, Ash1. Asterisks indicate the dominant mutant forms. Gray bars indicate the region of size overlap used for the analysis presented in <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1000221#pbio-1000221-t001\" target=\"_blank\">Table 1</a>.</p>", "links"=>[], "tags"=>["localization", "ace2", "ash1", "symmetric", "mothers"], "article_id"=>548836, "categories"=>["Cell Biology", "Developmental Biology"], "users"=>["Stefano Di Talia", "Hongyin Wang", "Jan M. Skotheim", "Adam P. Rosebrock", "Bruce Futcher", "Frederick R. Cross"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1000221.g002", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Symmetric_localization_of_Ace2_and_Ash1_result_in_symmetric_control_of_Start_in_mothers_and_daughters_/548836", "title"=>"Symmetric localization of Ace2 and Ash1 result in symmetric control of Start in mothers and daughters.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-10-20 02:27:16"}
  • {"files"=>["https://ndownloader.figshare.com/files/878535"], "description"=>"<p>Correlation between αT<sub>1</sub> and ln(M<sub>birth</sub>) for mothers and “pseudo-mothers” (in (A) cells grown in glucose, in (C) cells grown in glycerol/ethanol) and daughters and “pseudo-daughters” (in (B) cells grown in glucose, in (D) cells grown in glycerol/ethanol). (E) Correlation between αT<sub>1</sub> and ln(M<sub>birth</sub>) for mothers and “pseudo-mothers” grown in glucose and glycerol/ethanol (pulling together data from (A) and (C)). (B) Correlation between αT<sub>1</sub> and ln(M<sub>birth</sub>) for daughters and “pseudo-daughters” grown in glucose and glycerol/ethanol (pulling together data from (B) and (D)). (G) Correlation between αT<sub>1</sub> and ln(M<sub>birth</sub>) in mother-like and daughter-like cells. The graphs are obtained by binning all the data shown in (E) and (F). Error bars are standard errors of the mean. (H) Conditional probability of Whi5 nuclear exit as a function of ln(M) from data in (G). f is the probability that Whi5 will exit the nucleus at size ln(M) given that it had not exited at a smaller size.</p>", "links"=>[], "tags"=>["localization", "ace2", "ash1", "asymmetric"], "article_id"=>548985, "categories"=>["Cell Biology", "Developmental Biology"], "users"=>["Stefano Di Talia", "Hongyin Wang", "Jan M. Skotheim", "Adam P. Rosebrock", "Bruce Futcher", "Frederick R. Cross"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1000221.g003", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Daughter_specific_localization_of_Ace2_and_Ash1_results_in_asymmetric_cell_size_control_/548985", "title"=>"Daughter-specific localization of Ace2 and Ash1 results in asymmetric cell size control.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-10-20 02:29:45"}

PMC Usage Stats | Further Information

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  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"25", "unique-ip"=>"26", "pdf"=>"6", "year"=>"2010", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"30", "year"=>"2010", "pdf"=>"12", "unique-ip"=>"27", "figure"=>"15", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"5", "full-text"=>"23", "unique-ip"=>"26", "pdf"=>"12", "year"=>"2010", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"5", "full-text"=>"36", "year"=>"2010", "pdf"=>"12", "unique-ip"=>"27", "figure"=>"19", "scanned-summary"=>"0", "supp-data"=>"14"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"26", "unique-ip"=>"19", "pdf"=>"13", "year"=>"2010", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"4", "full-text"=>"23", "year"=>"2010", "pdf"=>"12", "unique-ip"=>"22", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"11", "unique-ip"=>"14", "pdf"=>"13", "year"=>"2010", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"15", "year"=>"2010", "pdf"=>"13", "unique-ip"=>"18", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"3"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"17", "unique-ip"=>"20", "pdf"=>"10", "year"=>"2010", "figure"=>"6", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"2", "abstract"=>"1", "full-text"=>"19", "year"=>"2010", "pdf"=>"8", "unique-ip"=>"23", "figure"=>"16", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"16", "unique-ip"=>"23", "pdf"=>"6", "year"=>"2011", "figure"=>"17", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"13", "year"=>"2011", "pdf"=>"9", "unique-ip"=>"22", "figure"=>"15", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"13", "unique-ip"=>"21", "pdf"=>"12", "year"=>"2011", "figure"=>"9", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"12", "year"=>"2011", "pdf"=>"8", "unique-ip"=>"20", "figure"=>"11", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"14", "unique-ip"=>"18", "pdf"=>"12", "year"=>"2011", "figure"=>"14", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"4", "full-text"=>"9", "year"=>"2011", "pdf"=>"8", "unique-ip"=>"17", "figure"=>"9", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"10", "unique-ip"=>"14", "pdf"=>"5", "year"=>"2011", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"12", "year"=>"2011", "pdf"=>"10", "unique-ip"=>"15", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"6", "unique-ip"=>"9", "pdf"=>"3", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"7", "year"=>"2011", "pdf"=>"3", "unique-ip"=>"8", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"18", "unique-ip"=>"12", "pdf"=>"2", "year"=>"2011", "figure"=>"6", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"26", "year"=>"2011", "pdf"=>"6", "unique-ip"=>"11", "figure"=>"6", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"7", "unique-ip"=>"6", "pdf"=>"2", "year"=>"2012", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"10", "year"=>"2012", "pdf"=>"4", "unique-ip"=>"11", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"14", "unique-ip"=>"16", "pdf"=>"15", "year"=>"2012", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"7", "year"=>"2012", "pdf"=>"2", "unique-ip"=>"8", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"6", "unique-ip"=>"8", "pdf"=>"3", "year"=>"2012", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"11", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"57", "year"=>"2009", "pdf"=>"12", "unique-ip"=>"52", "figure"=>"20", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"12", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"25", "unique-ip"=>"26", "pdf"=>"2", "year"=>"2009", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"5", "year"=>"2012", "pdf"=>"4", "unique-ip"=>"7", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"0", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"28", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"16", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"18", "full-text"=>"32", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"17", "full-text"=>"21", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"1", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
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  • {"unique-ip"=>"13", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"1", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
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Relative Metric

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