Statistical Analysis of Readthrough Levels for Nonsense Mutations in Mammalian Cells Reveals a Major Determinant of Response to Gentamicin
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Statistical analysis of readthrough levels for nonsense mutations in mammalian cells reveals a major determinant of response to gentamicin", "type"=>"journal", "authors"=>[{"first_name"=>"Célia", "last_name"=>"Floquet", "scopus_author_id"=>"6504795795"}, {"first_name"=>"Isabelle", "last_name"=>"Hatin", "scopus_author_id"=>"36845097300"}, {"first_name"=>"Jean Pierre", "last_name"=>"Rousset", "scopus_author_id"=>"7005138880"}, {"first_name"=>"Laure", "last_name"=>"Bidou", "scopus_author_id"=>"6602244560"}], "year"=>2012, "source"=>"PLoS Genetics", "identifiers"=>{"sgr"=>"84859239887", "scopus"=>"2-s2.0-84859239887", "doi"=>"10.1371/journal.pgen.1002608", "isbn"=>"1553-7404 (Electronic)\\r1553-7390 (Linking)", "pui"=>"364556426", "issn"=>"15537390", "pmid"=>"22479203"}, "id"=>"e4a7798c-93c4-3989-815b-513179fa2b67", "abstract"=>"The efficiency of translation termination depends on the nature of the stop codon and the surrounding nucleotides. Some molecules, such as aminoglycoside antibiotics (gentamicin), decrease termination efficiency and are currently being evaluated for diseases caused by premature termination codons. However, the readthrough response to treatment is highly variable and little is known about the rules governing readthrough level and response to aminoglycosides. In this study, we carried out in-depth statistical analysis on a very large set of nonsense mutations to decipher the elements of nucleotide context responsible for modulating readthrough levels and gentamicin response. We quantified readthrough for 66 sequences containing a stop codon, in the presence and absence of gentamicin, in cultured mammalian cells. We demonstrated that the efficiency of readthrough after treatment is determined by the complex interplay between the stop codon and a larger sequence context. There was a strong positive correlation between basal and induced readthrough levels, and a weak negative correlation between basal readthrough level and gentamicin response (i.e. the factor of increase from basal to induced readthrough levels). The identity of the stop codon did not affect the response to gentamicin treatment. In agreement with a previous report, we confirm that the presence of a cytosine in +4 position promotes higher basal and gentamicin-induced readthrough than other nucleotides. We highlight for the first time that the presence of a uracil residue immediately upstream from the stop codon is a major determinant of the response to gentamicin. Moreover, this effect was mediated by the nucleotide itself, rather than by the amino-acid or tRNA corresponding to the -1 codon. Finally, we point out that a uracil at this position associated with a cytosine at +4 results in an optimal gentamicin-induced readthrough, which is the therapeutically relevant variable.", "link"=>"http://www.mendeley.com/research/statistical-analysis-readthrough-levels-nonsense-mutations-mammalian-cells-reveals-major-determinant", "reader_count"=>53, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Student > Doctoral Student"=>3, "Researcher"=>13, "Student > Ph. D. Student"=>14, "Student > Postgraduate"=>1, "Student > Master"=>10, "Other"=>2, "Student > Bachelor"=>6, "Lecturer > Senior Lecturer"=>1, "Professor"=>2}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Student > Doctoral Student"=>3, "Researcher"=>13, "Student > Ph. D. Student"=>14, "Student > Postgraduate"=>1, "Student > Master"=>10, "Other"=>2, "Student > Bachelor"=>6, "Lecturer > Senior Lecturer"=>1, "Professor"=>2}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>10, "Mathematics"=>1, "Agricultural and Biological Sciences"=>28, "Medicine and Dentistry"=>7, "Chemistry"=>6, "Psychology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>7}, "Chemistry"=>{"Chemistry"=>6}, "Psychology"=>{"Psychology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>28}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>10}, "Mathematics"=>{"Mathematics"=>1}}, "reader_count_by_country"=>{"United States"=>1, "Brazil"=>1, "United Kingdom"=>2, "South Africa"=>1, "Germany"=>1}, "group_count"=>0}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84859239887"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84859239887?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84859239887&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84859239887&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84859239887", "dc:identifier"=>"SCOPUS_ID:84859239887", "eid"=>"2-s2.0-84859239887", "dc:title"=>"Statistical analysis of readthrough levels for nonsense mutations in mammalian cells reveals a major determinant of response to gentamicin", "dc:creator"=>"Floquet C.", "prism:publicationName"=>"PLoS Genetics", "prism:issn"=>"15537390", "prism:eIssn"=>"15537404", "prism:volume"=>"8", "prism:issueIdentifier"=>"3", "prism:pageRange"=>nil, "prism:coverDate"=>"2012-03-01", "prism:coverDisplayDate"=>"March 2012", "prism:doi"=>"10.1371/journal.pgen.1002608", "citedby-count"=>"31", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Universite Paris-Sud XI", "affiliation-city"=>"Orsay", "affiliation-country"=>"France"}, {"@_fa"=>"true", "affilname"=>"CNRS Centre National de la Recherche Scientifique", "affiliation-city"=>"Paris", "affiliation-country"=>"France"}], "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e1002608", "source-id"=>"4000151808"}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosgenetics%2Farticle%3Fid%3D10.1371%252Fjournal.pgen.1002608", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"36", "xml_views"=>"8", "html_views"=>"176"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"86", "xml_views"=>"23", "html_views"=>"466"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"33", "xml_views"=>"1", "html_views"=>"130"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"17", "xml_views"=>"4", "html_views"=>"57"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"13", "xml_views"=>"5", "html_views"=>"68"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"45"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"2", "html_views"=>"181"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"24", "xml_views"=>"1", "html_views"=>"66"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"86"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"91"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"71"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"53"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"14", "xml_views"=>"3", "html_views"=>"83"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"2", "html_views"=>"93"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"97"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"2", "html_views"=>"101"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"101"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"80"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"60"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"3", "html_views"=>"54"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"60"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"48"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"72"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"41"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"12"}

Figshare

  • {"files"=>["https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/338938/Figure_S1.tif", "https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/338967/Figure_S2.tif", "https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/339012/Figure_S3.tif", "https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/339052/Table_S1.pdf", "https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/339128/Table_S2.pdf", "https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/339175/Table_S3.pdf", "https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/339230/Table_S4.pdf", "https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/339297/Table_S5.pdf", "https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/339363/Table_S6.doc", "https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/339445/Table_S7.doc", "https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/339491/Table_S8.doc", "https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/339604/Table_S9.doc"], "description"=>"<div><p>The efficiency of translation termination depends on the nature of the stop codon and the surrounding nucleotides. Some molecules, such as aminoglycoside antibiotics (gentamicin), decrease termination efficiency and are currently being evaluated for diseases caused by premature termination codons. However, the readthrough response to treatment is highly variable and little is known about the rules governing readthrough level and response to aminoglycosides. In this study, we carried out in-depth statistical analysis on a very large set of nonsense mutations to decipher the elements of nucleotide context responsible for modulating readthrough levels and gentamicin response. We quantified readthrough for 66 sequences containing a stop codon, in the presence and absence of gentamicin, in cultured mammalian cells. We demonstrated that the efficiency of readthrough after treatment is determined by the complex interplay between the stop codon and a larger sequence context. There was a strong positive correlation between basal and induced readthrough levels, and a weak negative correlation between basal readthrough level and gentamicin response (i.e. the factor of increase from basal to induced readthrough levels). The identity of the stop codon did not affect the response to gentamicin treatment. In agreement with a previous report, we confirm that the presence of a cytosine in +4 position promotes higher basal and gentamicin-induced readthrough than other nucleotides. We highlight for the first time that the presence of a uracil residue immediately upstream from the stop codon is a major determinant of the response to gentamicin. Moreover, this effect was mediated by the nucleotide itself, rather than by the amino-acid or tRNA corresponding to the −1 codon. Finally, we point out that a uracil at this position associated with a cytosine at +4 results in an optimal gentamicin-induced readthrough, which is the therapeutically relevant variable.</p> </div>", "links"=>[], "tags"=>["readthrough", "levels", "nonsense", "mutations", "mammalian", "cells", "reveals", "determinant", "gentamicin"], "article_id"=>126998, "categories"=>["Mathematics", "Genetics"], "users"=>["Célia Floquet", "Isabelle Hatin", "Jean-Pierre Rousset", "Laure Bidou"], "doi"=>["http://dx.doi.org/10.1371/journal.pgen.1002608"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"http://figshare.com/articles/Statistical_Analysis_of_Readthrough_Levels_for_Nonsense_Mutations_in_Mammalian_Cells_Reveals_a_Major_Determinant_of_Response_to_Gentamicin/126998", "title"=>"Statistical Analysis of Readthrough Levels for Nonsense Mutations in Mammalian Cells Reveals a Major Determinant of Response to Gentamicin", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-03-29 01:56:38"}
  • {"files"=>["https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/660365/Figure_1.tif"], "description"=>"<p>Readthrough efficiencies of 66 sequences (65 nonsense mutations and 1 natural stop codon) were measured in NIH3T3 cells with and without gentamicin (800 µg/ml) treatment for 24 H. Each value shown is the mean of at least three independent experiments. For each sequence, the factor of increase (I) is the ratio of the gentamicin-induced readthrough level (G) to the basal readthrough level (B). Sequences are ranked in descending order of gentamicin-induced readthrough level.</p>", "links"=>[], "tags"=>["levels", "66", "sequences", "containing"], "article_id"=>330854, "categories"=>["Mathematics", "Genetics"], "users"=>["Célia Floquet", "Isabelle Hatin", "Jean-Pierre Rousset", "Laure Bidou"], "doi"=>["http://dx.doi.org/10.1371/journal.pgen.1002608.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"http://figshare.com/articles/_Readthrough_levels_and_factor_of_increase_for_66_sequences_containing_a_stop_codon_/330854", "title"=>"Readthrough levels and factor of increase for 66 sequences containing a stop codon.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-03-29 00:14:14"}
  • {"files"=>["https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/660451/Figure_2.tif"], "description"=>"<p>For each parameter, values are classified into intervals of equal size. X-values indicate the limits for intervals. Y-values indicate the number of values in each interval.</p>", "links"=>[], "tags"=>["representations", "parameters"], "article_id"=>330944, "categories"=>["Mathematics", "Genetics"], "users"=>["Célia Floquet", "Isabelle Hatin", "Jean-Pierre Rousset", "Laure Bidou"], "doi"=>["http://dx.doi.org/10.1371/journal.pgen.1002608.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"http://figshare.com/articles/_Graphical_representations_of_the_distribution_of_values_for_the_parameters_B_G_and_I_/330944", "title"=>"Graphical representations of the distribution of values for the parameters B, G, and I.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-03-29 00:15:44"}
  • {"files"=>["https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/660534/Figure_3.tif"], "description"=>"<p>Gentamicin-induced readthrough level is plotted against basal readthrough level (A); the factor of increase is plotted against basal readthrough level (B) and against gentamicin-induced readthrough level (C). Bravais-Pearson tests were performed to analyze the correlations between the three variables after Box-Cox transformation. Statistically significant results are indicated in <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002608#pgen.1002608.s007\" target=\"_blank\">Table S4</a>.</p>", "links"=>[], "tags"=>["representations", "variables", "box-cox"], "article_id"=>331023, "categories"=>["Mathematics", "Genetics"], "users"=>["Célia Floquet", "Isabelle Hatin", "Jean-Pierre Rousset", "Laure Bidou"], "doi"=>["http://dx.doi.org/10.1371/journal.pgen.1002608.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"http://figshare.com/articles/_Graphic_representations_of_correlation_between_the_variables_B_G_and_I_after_Box_Cox_transformation_/331023", "title"=>"Graphic representations of correlation between the variables B, G, and I after Box-Cox transformation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-03-29 00:17:03"}
  • {"files"=>["https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/660640/Figure_4.tif"], "description"=>"<p>Description of the statistical approach used for the study of the impact of identity of the stop codon or nucleotide context on readthrough levels and response to gentamicin.</p>", "links"=>[], "tags"=>["genetics and genomics", "mathematics"], "article_id"=>331129, "categories"=>["Mathematics", "Genetics"], "users"=>["Célia Floquet", "Isabelle Hatin", "Jean-Pierre Rousset", "Laure Bidou"], "doi"=>["http://dx.doi.org/10.1371/journal.pgen.1002608.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"http://figshare.com/articles/_Statistical_approach_/331129", "title"=>"Statistical approach.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-03-29 00:18:49"}
  • {"files"=>["https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/660733/Figure_5.tif"], "description"=>"<p>Graphical representation of medians (before Box-Cox transformation) for the parameters B, G and I are shown for the three different stop codons: UAA, UAG and UGA. The results of the statistical analysis after Box-Cox transformation are shown in <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002608#pgen.1002608.s008\" target=\"_blank\">Table S5</a>. For basal and gentamicin-induced readthrough levels, the following hierarchy of readthrough level was established: UGA>UAG>UAA. (with>indicating a significant difference). For the factor of increase, UGA = UAG = UAA.</p>", "links"=>[], "tags"=>["codon", "readthrough", "levels"], "article_id"=>331225, "categories"=>["Mathematics", "Genetics"], "users"=>["Célia Floquet", "Isabelle Hatin", "Jean-Pierre Rousset", "Laure Bidou"], "doi"=>["http://dx.doi.org/10.1371/journal.pgen.1002608.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"http://figshare.com/articles/_Effect_of_stop_codon_identity_on_readthrough_levels_and_the_factor_of_increase_/331225", "title"=>"Effect of stop codon identity on readthrough levels and the factor of increase.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-03-29 00:20:25"}
  • {"files"=>["https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/660784/Figure_6.tif"], "description"=>"<p>Graphical representations of medians for the parameters B, G and I (before Box-Cox transformation) for the four different nucleotides (A, C, G or U) at each position in the sequence surrounding the codon stop (−6 to +9). Statistical analysis was performed after Box-Cox transformation and results are summarized in <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002608#pgen-1002608-t001\" target=\"_blank\">Table 1</a> and detailed in <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002608#pgen.1002608.s009\" target=\"_blank\">Table S6</a>.</p>", "links"=>[], "tags"=>["nucleotide", "readthrough", "levels"], "article_id"=>331273, "categories"=>["Mathematics", "Genetics"], "users"=>["Célia Floquet", "Isabelle Hatin", "Jean-Pierre Rousset", "Laure Bidou"], "doi"=>["http://dx.doi.org/10.1371/journal.pgen.1002608.g006"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"http://figshare.com/articles/_Effect_of_nucleotide_identity_at_each_position_8722_6_to_9_on_readthrough_levels_and_the_factor_of_increase_/331273", "title"=>"Effect of nucleotide identity at each position (−6 to +9) on readthrough levels and the factor of increase.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-03-29 00:21:13"}
  • {"files"=>["https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/660933/Figure_7.tif"], "description"=>"<p>For DMD 319 and DMD 673, a U residue immediately upstream from the stop codon (−1) was replaced by each of the other nucleotides, separately. For DMD 931, p53 146, beta 17 and CF 122, the original nucleotide in the −1 position was replaced by a U residue. Readthrough efficiencies were measured as described in <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002608#pgen-1002608-g001\" target=\"_blank\">Figure 1</a>. Each value shown is the mean of at least five independent experiments. The original nucleotide is underlined on each graph.</p>", "links"=>[], "tags"=>["nucleotide", "upstream", "codon", "readthrough", "levels"], "article_id"=>331420, "categories"=>["Mathematics", "Genetics"], "users"=>["Célia Floquet", "Isabelle Hatin", "Jean-Pierre Rousset", "Laure Bidou"], "doi"=>["http://dx.doi.org/10.1371/journal.pgen.1002608.g007"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"http://figshare.com/articles/_Effect_of_the_nucleotide_upstream_from_the_stop_codon_8722_1_on_readthrough_levels_and_response_to_gentamicin_/331420", "title"=>"Effect of the nucleotide upstream from the stop codon (−1) on readthrough levels and response to gentamicin.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-03-29 00:23:40"}
  • {"files"=>["https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/661057/Figure_8.tif"], "description"=>"<p>The APC 1114 and APC 1131 nonsense mutations originally had a U residue in the −1 position, which we replaced with a C residue. For each sequence, the two different codons at the P-site are recognized by the same tRNA (<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002608#pgen-1002608-t002\" target=\"_blank\">Table 2</a>). Readthrough efficiencies were measured as described in <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002608#pgen-1002608-g001\" target=\"_blank\">Figure 1</a>. Each value shown is the mean of at least five independent experiments. The original nucleotide is underlined on each graph.</p>", "links"=>[], "tags"=>["trna", "determinant", "gentamicin"], "article_id"=>331548, "categories"=>["Mathematics", "Genetics"], "users"=>["Célia Floquet", "Isabelle Hatin", "Jean-Pierre Rousset", "Laure Bidou"], "doi"=>["http://dx.doi.org/10.1371/journal.pgen.1002608.g008"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"http://figshare.com/articles/_The_nature_of_the_tRNA_at_the_P_site_is_not_the_major_determinant_of_the_gentamicin_response_/331548", "title"=>"The nature of the tRNA at the P site is not the major determinant of the gentamicin response.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-03-29 00:25:48"}
  • {"files"=>["https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/661107/Table_1.xls"], "description"=>"<p>Hierarchies were established based on ANOVA/LSD tests after normalization of B, G and I. The sign>indicates a higher level of readthrough or factor of increase. In bold results obtained with a p≤0.05 (<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002608#pgen.1002608.s008\" target=\"_blank\">Table S5</a> and <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002608#pgen.1002608.s009\" target=\"_blank\">Table S6</a>).</p>*<p>p≤0.005.</p>", "links"=>[], "tags"=>["nucleotide", "codon", "basal", "readthrough", "gentamicin-induced"], "article_id"=>331594, "categories"=>["Mathematics", "Genetics"], "users"=>["Célia Floquet", "Isabelle Hatin", "Jean-Pierre Rousset", "Laure Bidou"], "doi"=>["http://dx.doi.org/10.1371/journal.pgen.1002608.t001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"http://figshare.com/articles/_Effects_of_the_nucleotide_context_and_the_stop_codon_on_basal_readthrough_B_gentamicin_induced_readthrough_G_and_increase_factor_I_/331594", "title"=>"Effects of the nucleotide context and the stop codon on basal readthrough (B), gentamicin-induced readthrough (G) and increase factor (I).", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-03-29 00:26:34"}
  • {"files"=>["https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/661140/Table_2.xls"], "description"=>"<p>Codons at −1 of these sequences are recognized by the same tRNA. In bold, the original nucleotide at −1 position and the Wobble pairing.</p>", "links"=>[], "tags"=>["anticodon", "upstream", "codon"], "article_id"=>331631, "categories"=>["Mathematics", "Genetics"], "users"=>["Célia Floquet", "Isabelle Hatin", "Jean-Pierre Rousset", "Laure Bidou"], "doi"=>["http://dx.doi.org/10.1371/journal.pgen.1002608.t002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"http://figshare.com/articles/_Codon_and_anticodon_immediately_upstream_of_the_stop_codon_8722_1_position_/331631", "title"=>"Codon and anticodon immediately upstream of the stop codon (−1 position).", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-03-29 00:27:11"}

PMC Usage Stats | Further Information

  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"29", "year"=>"2012", "pdf"=>"18", "unique-ip"=>"28", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"45", "unique-ip"=>"33", "pdf"=>"19", "year"=>"2012", "figure"=>"25", "scanned-summary"=>"0", "supp-data"=>"25"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"26", "year"=>"2012", "pdf"=>"15", "unique-ip"=>"23", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"16", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"17", "full-text"=>"23", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"18", "full-text"=>"19", "pdf"=>"6", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"16", "full-text"=>"20", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"6", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"2", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"16", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"14", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"19", "full-text"=>"25", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"9", "pdf"=>"6", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"20", "full-text"=>"22", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"20", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"28", "full-text"=>"28", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"30", "full-text"=>"63", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"31", "full-text"=>"45", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"15", "full-text"=>"27", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"15", "full-text"=>"19", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"24", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"17", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"11", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"1", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"6", "cited-by"=>"1", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}

Relative Metric

{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[322, 550, 671, 773, 864, 955, 1048, 1135, 1223, 1308, 1387, 1465, 1534, 1602, 1673, 1744, 1813, 1885, 1955, 2026, 2093, 2160, 2228, 2290, 2349]}, {"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[316, 541, 663, 766, 856, 950, 1041, 1128, 1218, 1302, 1382, 1456, 1526, 1593, 1657, 1729, 1796, 1862, 1930, 1999, 2065, 2132, 2202, 2261, 2319]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[333, 576, 707, 814, 908, 1004, 1104, 1197, 1280, 1370, 1449, 1531, 1603, 1673, 1742, 1817, 1886, 1954, 2025, 2098, 2171, 2234, 2304, 2365, 2431]}, {"subject_area"=>"/Physical sciences/Mathematics", "average_usage"=>[325, 522, 627, 718, 804, 884, 969, 1052, 1131, 1207, 1277, 1346, 1415, 1478, 1542, 1605, 1663, 1723, 1776, 1839, 1895, 1955, 2008, 2066, 2123]}]}
Loading … Spinner
There are currently no alerts