Double-Detargeted Oncolytic Adenovirus Shows Replication Arrest in Liver Cells and Retains Neuroendocrine Cell Killing Ability
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Double-detargeted oncolytic adenovirus shows replication arrest in liver cells and retains neuroendocrine cell killing ability", "type"=>"journal", "authors"=>[{"first_name"=>"Justyna", "last_name"=>"Leja", "scopus_author_id"=>"16301194400"}, {"first_name"=>"Berith", "last_name"=>"Nilsson", "scopus_author_id"=>"9336456200"}, {"first_name"=>"Di", "last_name"=>"Yu", "scopus_author_id"=>"55481576000"}, {"first_name"=>"Elisabet", "last_name"=>"Gustafson", "scopus_author_id"=>"16301659700"}, {"first_name"=>"Göran", "last_name"=>"Åkerström", "scopus_author_id"=>"7006226368"}, {"first_name"=>"Kjell", "last_name"=>"Öberg", "scopus_author_id"=>"7102283805"}, {"first_name"=>"Valeria", "last_name"=>"Giandomenico", "scopus_author_id"=>"6602894387"}, {"first_name"=>"Magnus", "last_name"=>"Essand", "scopus_author_id"=>"6701348777"}], "year"=>2010, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-77749270545", "doi"=>"10.1371/journal.pone.0008916", "issn"=>"19326203", "pui"=>"358424349", "sgr"=>"77749270545", "isbn"=>"1932-6203", "pmid"=>"20111709"}, "id"=>"505db7d4-e234-357d-b2f9-0fc10c2d78f2", "abstract"=>"BACKGROUND: We have previously developed an oncolytic serotype 5 adenovirus (Ad5) with chromogranin-A (CgA) promoter-controlled E1A expression, Ad[CgA-E1A], with the intention to treat neuroendocrine tumors, including carcinoids. Since carcinoids tend to metastasize to the liver it is important to fully repress viral replication in hepatocytes to avoid adenovirus-related liver toxicity. Herein, we explore miRNA-based regulation of E1A expression as a complementary mechanism to promoter-based transcriptional control.\\n\\nMETHODOLOGY/PRINCIPAL FINDINGS: Ad[CgA-E1A-miR122], where E1A expression is further controlled by six tandem repeats of the target sequence for the liver-specific miR122, was constructed and compared to Ad[CgA-E1A]. We observed E1A suppression and replication arrest of the miR122-detargeted adenovirus in normal hepatocytes, while the two viruses killed carcinoid cells to the same degree. Repeated intravenous injections of Ad[CgA-E1A] induced liver toxicity in mice while Ad[CgA-E1A-miR122] injections did not. Furthermore, a miR122-detargeted adenovirus with the wild-type E1A promoter showed reduced replication in hepatic cells compared to wild-type Ad5 but not to the same extent as the miR122-detargeted adenovirus with the neuroendocrine-selective CgA promoter.\\n\\nCONCLUSIONS/SIGNIFICANCE: A combination of transcriptional (promoter) and post-transcriptional (miRNA target) regulation to control virus replication may allow for the use of higher doses of adenovirus for efficient tumors treatment without liver toxicity.", "link"=>"http://www.mendeley.com/research/doubledetargeted-oncolytic-adenovirus-shows-replication-arrest-liver-cells-retains-neuroendocrine-ce", "reader_count"=>28, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Librarian"=>1, "Researcher"=>8, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>4, "Student > Master"=>4, "Other"=>1, "Student > Bachelor"=>6, "Professor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Librarian"=>1, "Researcher"=>8, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>4, "Student > Master"=>4, "Other"=>1, "Student > Bachelor"=>6, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Nursing and Health Professions"=>1, "Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>16, "Medicine and Dentistry"=>7}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>7}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>16}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"United States"=>1, "Japan"=>1, "United Kingdom"=>1, "Australia"=>1, "Portugal"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/77749270545"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/77749270545?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77749270545&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=77749270545&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/77749270545", "dc:identifier"=>"SCOPUS_ID:77749270545", "eid"=>"2-s2.0-77749270545", "dc:title"=>"Double-detargeted oncolytic adenovirus shows replication arrest in liver cells and retains neuroendocrine cell killing ability", "dc:creator"=>"Leja J.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"5", "prism:issueIdentifier"=>"1", "prism:pageRange"=>nil, "prism:coverDate"=>"2010-01-27", "prism:coverDisplayDate"=>"27 January 2010", "prism:doi"=>"10.1371/journal.pone.0008916", "citedby-count"=>"37", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"University of Uppsala Rudbeck Laboratory", "affiliation-city"=>"Uppsala", "affiliation-country"=>"Sweden"}], "pubmed-id"=>"20111709", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e8916", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0008916", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"12", "year"=>"2009", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"1", "year"=>"2010", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"107"}
  • {"month"=>"2", "year"=>"2010", "pdf_views"=>"34", "xml_views"=>"0", "html_views"=>"87"}
  • {"month"=>"3", "year"=>"2010", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"4", "year"=>"2010", "pdf_views"=>"24", "xml_views"=>"1", "html_views"=>"53"}
  • {"month"=>"5", "year"=>"2010", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"49"}
  • {"month"=>"6", "year"=>"2010", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"7", "year"=>"2010", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"8", "year"=>"2010", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"9", "year"=>"2010", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"52"}
  • {"month"=>"10", "year"=>"2010", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"53"}
  • {"month"=>"11", "year"=>"2010", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"12", "year"=>"2010", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"1", "year"=>"2011", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"21"}
  • {"month"=>"2", "year"=>"2011", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"35"}
  • {"month"=>"3", "year"=>"2011", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"4", "year"=>"2011", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"5", "year"=>"2011", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"29"}
  • {"month"=>"6", "year"=>"2011", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2011", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"8", "year"=>"2011", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"9", "year"=>"2011", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"10", "year"=>"2011", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"11", "year"=>"2011", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"6", "xml_views"=>"5", "html_views"=>"28"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"47"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"63"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"46"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"49"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"28"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"40"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"3", "html_views"=>"52"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"35"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"23"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"17"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"3", "html_views"=>"2"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"1"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"0"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/429649", "https://ndownloader.figshare.com/files/429719", "https://ndownloader.figshare.com/files/429762", "https://ndownloader.figshare.com/files/429797"], "description"=>"<div><h3>Background</h3><p>We have previously developed an oncolytic serotype 5 adenovirus (Ad5) with chromogranin-A (CgA) promoter-controlled E1A expression, Ad[CgA-E1A], with the intention to treat neuroendocrine tumors, including carcinoids. Since carcinoids tend to metastasize to the liver it is important to fully repress viral replication in hepatocytes to avoid adenovirus-related liver toxicity. Herein, we explore miRNA-based regulation of E1A expression as a complementary mechanism to promoter-based transcriptional control.</p><h3>Methodology/Principal Findings</h3><p>Ad[CgA-E1A-miR122], where E1A expression is further controlled by six tandem repeats of the target sequence for the liver-specific miR122, was constructed and compared to Ad[CgA-E1A]. We observed E1A suppression and replication arrest of the miR122-detargeted adenovirus in normal hepatocytes, while the two viruses killed carcinoid cells to the same degree. Repeated intravenous injections of Ad[CgA-E1A] induced liver toxicity in mice while Ad[CgA-E1A-miR122] injections did not. Furthermore, a miR122-detargeted adenovirus with the wild-type E1A promoter showed reduced replication in hepatic cells compared to wild-type Ad5 but not to the same extent as the miR122-detargeted adenovirus with the neuroendocrine-selective CgA promoter.</p><h3>Conclusions/Significance</h3><p>A combination of transcriptional (promoter) and post-transcriptional (miRNA target) regulation to control virus replication may allow for the use of higher doses of adenovirus for efficient tumors treatment without liver toxicity.</p></div>", "links"=>[], "tags"=>["double-detargeted", "oncolytic", "adenovirus", "shows", "replication", "cells", "retains", "neuroendocrine"], "article_id"=>144816, "categories"=>["Cancer"], "users"=>["Justyna Leja", "Berith Nilsson", "Di Yu", "Elisabet Gustafson", "Göran Åkerström", "Kjell Öberg", "Valeria Giandomenico", "Magnus Essand"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0008916.s001", "https://dx.doi.org/10.1371/journal.pone.0008916.s002", "https://dx.doi.org/10.1371/journal.pone.0008916.s003", "https://dx.doi.org/10.1371/journal.pone.0008916.s004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Double_Detargeted_Oncolytic_Adenovirus_Shows_Replication_Arrest_in_Liver_Cells_and_Retains_Neuroendocrine_Cell_Killing_Ability/144816", "title"=>"Double-Detargeted Oncolytic Adenovirus Shows Replication Arrest in Liver Cells and Retains Neuroendocrine Cell Killing Ability", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2010-01-27 01:20:16"}
  • {"files"=>["https://ndownloader.figshare.com/files/866236"], "description"=>"<p>Ad[CgA-Luc] and Ad[CgA-Luc-miR122] are E1-deleted Ad5-based vectors with luciferase gene expression controlled by the human CgA promoter. Ad[CgA-Luc-miR122] has six repeats of the miR122 target sequence in the 3′UTR of the luciferase gene. The oncolytic adenovirus Ad[CgA-E1A], which has been previously described, has E1A expression under control of the CgA promoter. Six and twelve repeats of the miR122 target sequence were inserted in the 3′UTR of the E1A gene in Ad[CgA-E1A-miR122] and Ad[CgA-E1A-miR122x2], respectively.</p>", "links"=>[], "tags"=>["recombinant"], "article_id"=>536693, "categories"=>["Cancer", "Infectious Diseases"], "users"=>["Justyna Leja", "Berith Nilsson", "Di Yu", "Elisabet Gustafson", "Göran Åkerström", "Kjell Öberg", "Valeria Giandomenico", "Magnus Essand"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0008916.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Schematic_illustration_of_recombinant_adenoviruses_/536693", "title"=>"Schematic illustration of recombinant adenoviruses.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-01-27 01:51:33"}
  • {"files"=>["https://ndownloader.figshare.com/files/866418"], "description"=>"<p>(A) Freshly isolated primary cells and cell lines were transduced with Ad[CgA-Luc] and Ad[CgA-Luc-miR122] at MOI 10 and plated in 12-well plates. Cells were harvested after 48 hours and luciferase activity and protein concentration were determined. Luciferase activity was calculated as RLU/mg and expressed in relation to Ad[CgA-Luc], which was set to 100% for each cell type. Data are presented as the mean values of triplicates ± std dev. (B-E) Balb/c mice were injected i.v. with 5×10<sup>10</sup>vp of Ad[CMV-Luc] (B), Ad[CgA-Luc] (C) or Ad[CgA-Luc-miR122] (D). Luciferase activity was monitored with the IVIS-100 system 6, 24 and 48 hours after injection. (E) Comparison of luciferase activity in mice liver after i.v. injection of Ad[CMV-Luc], Ad[CgA-Luc] or Ad[CgA-Luc-miR122] (*** p<0.0001).</p>", "links"=>[], "tags"=>["luciferase", "cells", "mir122"], "article_id"=>536875, "categories"=>["Cancer", "Infectious Diseases"], "users"=>["Justyna Leja", "Berith Nilsson", "Di Yu", "Elisabet Gustafson", "Göran Åkerström", "Kjell Öberg", "Valeria Giandomenico", "Magnus Essand"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0008916.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Specific_silencing_of_luciferase_expression_in_liver_cells_by_miR122_in_vitro_and_in_vivo_/536875", "title"=>"Specific silencing of luciferase expression in liver cells by miR122 <i>in vitro</i> and <i>in vivo</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-01-27 01:54:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/866526"], "description"=>"<p>Primary cells (A), hepatoma cell lines (B) and neuroendocrine tumor cell lines (C) were transduced with viruses at MOI 1 (neuroblastoma cell lines at MOI 10). Total protein lysates were prepared after 48 hours and 50 μg of samples were resolved by SDS-PAGE. E1A was detected by Western blotting using an anti-E1A antibody. The polyclonal anti-Ad serum (α-Prage) was used to detect adenoviral capsid proteins. Hexon protein expression is shown to visualize production of progeny virions.</p>", "links"=>[], "tags"=>["suppression", "adenoviral", "hepatic"], "article_id"=>536979, "categories"=>["Cancer", "Infectious Diseases"], "users"=>["Justyna Leja", "Berith Nilsson", "Di Yu", "Elisabet Gustafson", "Göran Åkerström", "Kjell Öberg", "Valeria Giandomenico", "Magnus Essand"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0008916.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_miR122_mediated_suppression_of_adenoviral_protein_expression_in_hepatic_cells_/536979", "title"=>"miR122-mediated suppression of adenoviral protein expression in hepatic cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-01-27 01:56:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/866657"], "description"=>"<p>(A) Cells were transduced with viruses at MOI 1 (neuroblastoma cell lines at MOI 10). Viral DNA was isolated 2 hours, 1, 2 and 3 days after transduction. QRT-PCR was performed to measure E4 orf1 gene copies. Fold increase of viral genome copies is expressed in relation to genome copies detected after 2 hours (set to 1). (B) HuH7.5 and BON cells were transduced with viruses at various MOIs (0.001-1). Cell viability was examined 6 days after transduction and expressed in relation to the viability of untransduced cells.</p>", "links"=>[], "tags"=>["reduced", "cytolytic", "hepatic", "cells", "adenoviruses", "carrying", "mir122"], "article_id"=>537105, "categories"=>["Cancer", "Infectious Diseases"], "users"=>["Justyna Leja", "Berith Nilsson", "Di Yu", "Elisabet Gustafson", "Göran Åkerström", "Kjell Öberg", "Valeria Giandomenico", "Magnus Essand"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0008916.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Replication_arrest_and_reduced_cytolytic_activity_in_hepatic_cells_for_adenoviruses_carrying_miR122_target_sites_/537105", "title"=>"Replication arrest and reduced cytolytic activity in hepatic cells for adenoviruses carrying miR122 target sites.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-01-27 01:58:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/866741"], "description"=>"<p>Balb/c mice received three intravenous injections with 5×10<sup>10</sup>vp of Ad[CgA-E1A] or Ad[CgA-E1A-miR122] on days 1, 4 and 7. Blood was collected on day 9 and plasma was obtained by incubation blood with heparin (20 U per ml of blood) and used for the measurement of ALT levels (*p = 0,0011).</p>", "links"=>[], "tags"=>["hepatotoxicity", "mice", "injected"], "article_id"=>537192, "categories"=>["Cancer", "Infectious Diseases"], "users"=>["Justyna Leja", "Berith Nilsson", "Di Yu", "Elisabet Gustafson", "Göran Åkerström", "Kjell Öberg", "Valeria Giandomenico", "Magnus Essand"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0008916.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Lack_of_hepatotoxicity_in_mice_injected_with_Ad_CgA_E1A_miR122_/537192", "title"=>"Lack of hepatotoxicity in mice injected with Ad[CgA-E1A-miR122].", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-01-27 01:59:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/866864"], "description"=>"<p>HuH7.5 (A) and BON (B) were transduced at MOI 1. Viral DNA was isolated 2 hours and 2 days after transduction. QRT-PCR was performed to measure E4 orf1 copies. Fold increase of viral genome copies after 2 days is expressed in relation to genome copies detected after 2 hours (set to 1). Data are presented as the mean of triplicates ± std dev.</p>", "links"=>[], "tags"=>["replication", "double-targeted", "single-targeted", "adenovirus", "hepatic"], "article_id"=>537304, "categories"=>["Cancer", "Infectious Diseases"], "users"=>["Justyna Leja", "Berith Nilsson", "Di Yu", "Elisabet Gustafson", "Göran Åkerström", "Kjell Öberg", "Valeria Giandomenico", "Magnus Essand"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0008916.g006"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Greater_replication_arrest_by_double_targeted_than_single_targeted_adenovirus_in_hepatic_cells_/537304", "title"=>"Greater replication arrest by double-targeted than single-targeted adenovirus in hepatic cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-01-27 02:01:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/866999"], "description"=>"<p>HuH7.5 (A) and BON (B) were transduced at various MOIs (0.0001-100). Cell viability was examined 6 (HuH7.5) and 4 (BON) days after transduction. Cell viability values were expressed in relation to the viability of untransduced cells. Average enzymatic activities from triplicate samples are shown.</p>", "links"=>[], "tags"=>["cytolytic", "hepatic", "cells", "double-targeted", "single-targeted"], "article_id"=>537449, "categories"=>["Cancer", "Infectious Diseases"], "users"=>["Justyna Leja", "Berith Nilsson", "Di Yu", "Elisabet Gustafson", "Göran Åkerström", "Kjell Öberg", "Valeria Giandomenico", "Magnus Essand"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0008916.g007"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Stronger_reduction_of_cytolytic_ability_in_hepatic_cells_by_double_targeted_than_single_targeted_adenovirus_/537449", "title"=>"Stronger reduction of cytolytic ability in hepatic cells by double-targeted than single-targeted adenovirus.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-01-27 02:04:09"}

PMC Usage Stats | Further Information

  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"2", "unique-ip"=>"4", "pdf"=>"0", "year"=>"2010", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"61", "year"=>"2010", "pdf"=>"44", "unique-ip"=>"53", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"32", "unique-ip"=>"29", "pdf"=>"23", "year"=>"2010", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"3"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"35", "year"=>"2010", "pdf"=>"23", "unique-ip"=>"30", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"5"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"8", "full-text"=>"23", "unique-ip"=>"28", "pdf"=>"13", "year"=>"2010", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"4", "full-text"=>"14", "year"=>"2010", "pdf"=>"12", "unique-ip"=>"18", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"5"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"4", "full-text"=>"13", "unique-ip"=>"15", "pdf"=>"8", "year"=>"2010", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"10", "year"=>"2010", "pdf"=>"5", "unique-ip"=>"13", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"13", "unique-ip"=>"18", "pdf"=>"8", "year"=>"2010", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"11", "year"=>"2010", "pdf"=>"9", "unique-ip"=>"13", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"10", "unique-ip"=>"17", "pdf"=>"8", "year"=>"2010", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"9", "year"=>"2010", "pdf"=>"9", "unique-ip"=>"11", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"10", "unique-ip"=>"13", "pdf"=>"11", "year"=>"2011", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"9", "year"=>"2011", "pdf"=>"3", "unique-ip"=>"13", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"10", "unique-ip"=>"12", "pdf"=>"6", "year"=>"2011", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"10", "year"=>"2011", "pdf"=>"2", "unique-ip"=>"10", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"6", "unique-ip"=>"10", "pdf"=>"4", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"4", "year"=>"2011", "pdf"=>"4", "unique-ip"=>"10", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"6", "unique-ip"=>"8", "pdf"=>"6", "year"=>"2011", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"15", "year"=>"2011", "pdf"=>"11", "unique-ip"=>"14", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"7", "unique-ip"=>"9", "pdf"=>"5", "year"=>"2011", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"12", "year"=>"2011", "pdf"=>"8", "unique-ip"=>"14", "figure"=>"14", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"11", "unique-ip"=>"9", "pdf"=>"5", "year"=>"2011", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"3"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"3", "year"=>"2011", "pdf"=>"1", "unique-ip"=>"5", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"10", "unique-ip"=>"7", "pdf"=>"5", "year"=>"2012", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"5", "year"=>"2012", "pdf"=>"3", "unique-ip"=>"6", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"8", "unique-ip"=>"12", "pdf"=>"41", "year"=>"2012", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"1", "year"=>"2012", "pdf"=>"27", "unique-ip"=>"2", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"2", "unique-ip"=>"5", "pdf"=>"6", "year"=>"2012", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"5", "year"=>"2012", "pdf"=>"1", "unique-ip"=>"4", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"21", "full-text"=>"20", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"26", "full-text"=>"20", "pdf"=>"8", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"25", "full-text"=>"22", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"1", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"3", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"14", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"5", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}

Relative Metric

{"start_date"=>"2010-01-01T00:00:00Z", "end_date"=>"2010-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Anatomy", "average_usage"=>[275, 532, 682, 800, 904, 1008, 1103, 1194, 1265, 1340, 1412, 1479, 1546, 1610, 1672, 1736, 1793, 1861, 1922, 1978, 2032, 2094, 2155, 2220, 2279, 2333, 2395, 2481, 2543, 2601, 2669, 2722, 2767, 2826, 2882, 2942, 3006, 3054, 3110, 3176, 3253, 3313, 3367, 3422, 3472, 3539, 3593, 3655, 3703]}, {"subject_area"=>"/Biology and life sciences/Anatomy and physiology", "average_usage"=>[276, 544, 702, 826, 936, 1045, 1140, 1222, 1305, 1377, 1453, 1528, 1584, 1661, 1725, 1783, 1848, 1913, 1977, 2038, 2103, 2158, 2223, 2290, 2346, 2406, 2467, 2529, 2588, 2652, 2722, 2779, 2835, 2892, 2943, 2999]}, {"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[283, 565, 720, 852, 970, 1070, 1168, 1258, 1336, 1411, 1487, 1571, 1640, 1709, 1776, 1843, 1906, 1969, 2031, 2086, 2152, 2221, 2286, 2361, 2419, 2480, 2543, 2598, 2665, 2731, 2802, 2868, 2932, 2996, 3053, 3114, 3169, 3232, 3289, 3355, 3413, 3473, 3530, 3595, 3648, 3711, 3764, 3810, 3863]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[280, 562, 725, 863, 974, 1083, 1177, 1268, 1347, 1421, 1489, 1570, 1638, 1706, 1763, 1823, 1890, 1951, 2016, 2076, 2134, 2192, 2257, 2319, 2378, 2438, 2501, 2572, 2634, 2700, 2759, 2825, 2887, 2936, 3007, 3070, 3121, 3184, 3237, 3304, 3363, 3425, 3484, 3531, 3612, 3663, 3718, 3771]}, {"subject_area"=>"/Biology and life sciences/Microbiology", "average_usage"=>[306, 616, 785, 930, 1062, 1178, 1267, 1361, 1440, 1545, 1627, 1690, 1764, 1825, 1903, 1965, 2043, 2099, 2172, 2232, 2313, 2376, 2451, 2524, 2595, 2661, 2729, 2781, 2848, 2911, 2974, 3059, 3138, 3196, 3272, 3342, 3408, 3471, 3530, 3601, 3661, 3734, 3803, 3862, 3922, 3966, 4035, 4092, 4165]}, {"subject_area"=>"/Medicine and health sciences/Anatomy", "average_usage"=>[268, 527, 681, 804, 923, 1031, 1126, 1210, 1284, 1361, 1440, 1508, 1578, 1631, 1688, 1754, 1816, 1877, 1945, 2003, 2060, 2125, 2183, 2253, 2313, 2379, 2436, 2513, 2570, 2626, 2702, 2757, 2809, 2885, 2945, 3006, 3067, 3128, 3180, 3237, 3290, 3347, 3397, 3462, 3520, 3585, 3651, 3706, 3755]}, {"subject_area"=>"/Medicine and health sciences/Anatomy and physiology", "average_usage"=>[270, 536, 692, 812, 932, 1037, 1128, 1210, 1293, 1367, 1439, 1514, 1580, 1646, 1711, 1766, 1834, 1902, 1969, 2036, 2106, 2165, 2221, 2285, 2337, 2403, 2459, 2517, 2586, 2644, 2716, 2782, 2842, 2895, 2958, 3017]}, {"subject_area"=>"/Medicine and health sciences/Pathology and laboratory medicine", "average_usage"=>[274, 546, 703, 837, 951, 1058, 1152, 1234, 1323, 1387, 1453, 1538, 1606, 1694, 1753, 1814, 1873, 1941, 2011, 2079, 2137, 2193, 2260, 2325, 2386, 2459, 2528, 2598, 2651, 2728, 2788, 2859, 2931, 2999, 3068, 3131, 3209, 3258, 3321, 3372, 3426, 3500, 3561, 3613, 3685, 3743, 3805, 3839]}]}
Loading … Spinner
There are currently no alerts