PCR Master Mixes Harbour Murine DNA Sequences. Caveat Emptor!
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{"title"=>"PCR master mixes harbour murine DNA sequences. caveat emptor!", "type"=>"journal", "authors"=>[{"first_name"=>"Philip W.", "last_name"=>"Tuke", "scopus_author_id"=>"6602490740"}, {"first_name"=>"Kate I.", "last_name"=>"Tettmar", "scopus_author_id"=>"37063754500"}, {"first_name"=>"Asif", "last_name"=>"Tamuri", "scopus_author_id"=>"35590604000"}, {"first_name"=>"Jonathan P.", "last_name"=>"Stoye", "scopus_author_id"=>"7006553905"}, {"first_name"=>"Richard S.", "last_name"=>"Tedder", "scopus_author_id"=>"56945667500"}], "year"=>2011, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-79957450477", "doi"=>"10.1371/journal.pone.0019953", "pui"=>"361822285", "issn"=>"19326203", "pmid"=>"21647447", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "sgr"=>"79957450477"}, "id"=>"59d86d83-918b-36e8-9fab-68939cc04c7d", "abstract"=>"XMRV is the most recently described retrovirus to be found in Man, firstly in patients with prostate cancer (PC) and secondly in 67% of patients with chronic fatigue syndrome (CFS) and 3.7% of controls. Both disease associations remain contentious. Indeed, a recent publication has concluded that \"XMRV is unlikely to be a human pathogen\". Subsequently related but different polytropic MLV (pMLV) sequences were also reported from the blood of 86.5% of patients with CFS. and 6.8% of controls. Consequently we decided to investigate blood donors for evidence of XMRV/pMLV.", "link"=>"http://www.mendeley.com/research/pcr-master-mixes-harbour-murine-dna-sequences-caveat-emptor", "reader_count"=>22, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Student > Doctoral Student"=>1, "Researcher"=>5, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>1, "Student > Master"=>1, "Other"=>1, "Student > Bachelor"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Student > Doctoral Student"=>1, "Researcher"=>5, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>1, "Student > Master"=>1, "Other"=>1, "Student > Bachelor"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Medicine and Dentistry"=>1, "Agricultural and Biological Sciences"=>14, "Psychology"=>2, "Chemistry"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Psychology"=>{"Psychology"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>14}, "Unspecified"=>{"Unspecified"=>3}}, "reader_count_by_country"=>{"Argentina"=>1, "South Africa"=>1, "United Kingdom"=>1}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/771322"], "description"=>"<p><i>gag</i> nested PCR for 80 cDNAs derived from blood donors and controls. Lanes 1A–1H ten fold dilution series of XMRV TCS (Lane 1A equivalent to 5.5×10<sup>6</sup> to 5.5×10<sup>−1</sup> cDNA molecules/PCR – determined by Poisson distribution). Lanes 2A–11H 80 cDNAs derived from blood donor whole blood. Lanes 12A–12H ten fold dilution series of Balb/c DNA 10<sup>5</sup> pg to 0.10 pg, negative.</p>", "links"=>[], "tags"=>["gel", "r2"], "article_id"=>441689, "categories"=>["Virology", "Infectious Diseases"], "users"=>["Philip W. Tuke", "Kate I. Tettmar", "Asif Tamuri", "Jonathan P. Stoye", "Richard S. Tedder"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0019953.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Agarose_gel_analysis_of_R2_from_experiment_1_/441689", "title"=>"Agarose gel analysis of R2 from experiment 1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 18:04:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/771410"], "description"=>"<p>The MLV RAxML tree for gag sequences lying between XMRV primers gagIF and gagIR was developed from the endogenous MLV sequences identified by Jern et al <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0019953#pone.0019953-Jern1\" target=\"_blank\">[19]</a>, sequences derived from CFS patients by Lo et al <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0019953#pone.0019953-Lo1\" target=\"_blank\">[5]</a> (shown in red and blue) and the sequences amplified in this study (in green). In addition to sequences from the RNA and DNA, “1F_131010” and “1F” are included. 1F_131010 was derived from the amplification of cDNA synthesized from RNA extracted from the tissue culture fluid supernatant of an XMRV culture, kindly provided by Professor M McClure. 1F was derived from the amplification of Balb/c mouse DNA (Sigma). Bootstrap values >50 are indicated.</p>", "links"=>[], "tags"=>["gag"], "article_id"=>441780, "categories"=>["Virology", "Infectious Diseases"], "users"=>["Philip W. Tuke", "Kate I. Tettmar", "Asif Tamuri", "Jonathan P. Stoye", "Richard S. Tedder"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0019953.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Maximum_likelihood_tree_of_gag_sequences_/441780", "title"=>"Maximum likelihood tree of gag sequences.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 18:05:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/771525"], "description"=>"<p>The nine nucleotide differences between cfs1 and the other viral sequences are highlighted.</p>", "links"=>[], "tags"=>["pmv15"], "article_id"=>441897, "categories"=>["Virology", "Infectious Diseases"], "users"=>["Philip W. Tuke", "Kate I. Tettmar", "Asif Tamuri", "Jonathan P. Stoye", "Richard S. Tedder"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0019953.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Alignment_between_cfs1_MLV006_Pmv15_and_Pmv22_/441897", "title"=>"Alignment between cfs1, MLV006, Pmv15 and Pmv22.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 18:06:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/771596"], "description"=>"<p>400 nM concentrations of primers and 200 nM probes were used in all the TaqMan assays.</p>", "links"=>[], "tags"=>["pcrs"], "article_id"=>441966, "categories"=>["Virology", "Infectious Diseases"], "users"=>["Philip W. Tuke", "Kate I. Tettmar", "Asif Tamuri", "Jonathan P. Stoye", "Richard S. Tedder"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0019953.t001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Details_of_PCRs_performed_/441966", "title"=>"Details of PCRs performed.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-20 18:06:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/771635"], "description"=>"<p>WB = Whole Blood.</p>", "links"=>[], "tags"=>["sequences"], "article_id"=>442006, "categories"=>["Virology", "Infectious Diseases"], "users"=>["Philip W. Tuke", "Kate I. Tettmar", "Asif Tamuri", "Jonathan P. Stoye", "Richard S. Tedder"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0019953.t002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Details_of_sequences_obtained_/442006", "title"=>"Details of sequences obtained.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-20 18:06:42"}

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  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
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  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"5"}
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  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"15", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"1", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
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  • {"unique-ip"=>"2", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
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  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
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Relative Metric

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