Renoprotective Effect of Human Umbilical Cord–Derived Mesenchymal Stem Cells in Immunodeficient Mice Suffering from Acute Kidney Injury
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{"title"=>"Renoprotective Effect of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Immunodeficient Mice Suffering from Acute Kidney Injury", "type"=>"journal", "authors"=>[{"first_name"=>"Te Chao", "last_name"=>"Fang", "scopus_author_id"=>"55882132100"}, {"first_name"=>"Cheng Yoong", "last_name"=>"Pang", "scopus_author_id"=>"7201425141"}, {"first_name"=>"Sheng Chun", "last_name"=>"Chiu", "scopus_author_id"=>"26646335100"}, {"first_name"=>"Dah Ching", "last_name"=>"Ding", "scopus_author_id"=>"7201761056"}, {"first_name"=>"Rong Kung", "last_name"=>"Tsai", "scopus_author_id"=>"7103215741"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84866933457", "pui"=>"365755651", "doi"=>"10.1371/journal.pone.0046504", "issn"=>"19326203", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"23029541", "sgr"=>"84866933457"}, "id"=>"162cadec-75d4-3763-8a2e-5c223f2928e9", "abstract"=>"It is unknown whether human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) can improve the renal function of patients suffering from acute kidney injury. Moreover, before beginning clinical trials, it is necessary to investigate this renoprotective effect of hUC-MSCs in a xenogeneic model of acute kidney injury. However, no previous studies have examined the application of hUC-MSCs to immunodeficient mice suffering from acute kidney injury. The objectives of this study were to examine whether hUC-MSCs could improve renal function in nonobese diabetic-severe combined immune deficiency (NOD-SCID) mice suffering from acute kidney injury, and to investigate the mechanism(s) for hUC-MSCs to improve renal function in this xenogeneic model. Early (3 hr) and late (12 hr) administrations of hUC-MSCs (10(6) cells) were performed via the external jugular vein into NOD-SCID mice suffering from either folic acid (FA) (250 mg/kg body weight) or vehicle. The results showed that early administration of hUC-MSCs improved the renal function of NOD-SCID mice suffering from FA-induced acute kidney injury, as evidenced by decreased serum urea nitrogen and serum creatinine levels, as well as a reduced tubular injury score. The beneficial effects of hUC-MSCs were through reducing apoptosis and promoting proliferation of renal tubular cells. These benefits were independent of inflammatory cytokine effects and transdifferentiation. Furthermore, this study is the first one to show that the reduced apoptosis of renal tubular cells by hUC-MSCs in this xenogeneic model is mediated through the mitochondrial pathway, and through the increase of Akt phosphorylation.", "link"=>"http://www.mendeley.com/research/renoprotective-effect-human-umbilical-cordderived-mesenchymal-stem-cells-immunodeficient-mice-suffer", "reader_count"=>30, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Student > Doctoral Student"=>2, "Researcher"=>5, "Student > Ph. D. Student"=>7, "Student > Master"=>7, "Other"=>1, "Student > Bachelor"=>3, "Professor"=>4}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Student > Doctoral Student"=>2, "Researcher"=>5, "Student > Ph. D. Student"=>7, "Student > Master"=>7, "Other"=>1, "Student > Bachelor"=>3, "Professor"=>4}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>5, "Agricultural and Biological Sciences"=>15, "Medicine and Dentistry"=>9, "Neuroscience"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>9}, "Neuroscience"=>{"Neuroscience"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>15}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>5}}, "reader_count_by_country"=>{"Canada"=>1, "United States"=>1, "United Kingdom"=>1, "Australia"=>1, "Germany"=>1}, "group_count"=>1}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/569385"], "description"=>"<p>Folic acid injury increased the expression of the cleaved form of caspase-3 (19 KDa) and -9 (38 KDa), and subsequent treatment with hUC-MSCs decreased the expression of the cleaved forms of caspase-3 and -9. The sample size for each time frame per group was six mice and at least three independent experiments were performed. Abbreviations: NOD-SCID, nonobese diabetic-severe combined immunodeficiency disease; CON, control NOD-SCID mice; FA, NOD-SCID mice were treated with folic acid; FA+hUC-MSCs, NOD-SCID mice were treated with folic acid and then given hUC-MSCs 3 h later; CON+hUC-MSCs, NOD-SCID mice were given hUC-MSCs; MW, molecular weight; kDa, kilodalton.</p>", "links"=>[], "tags"=>["umbilical", "cord-derived", "mesenchymal", "cells", "cleaved", "-9", "expressions", "kidney", "nod-scid", "mice", "treated", "folic", "examples", "fluorescent", "immunohistochemistry", "staining", "renal", "tubular", "fa", "subsequently", "huc-mscs"], "article_id"=>239852, "categories"=>["Cell Biology", "Marine Biology", "Immunology"], "users"=>["Te-Chao Fang", "Cheng-Yoong Pang", "Sheng-Chun Chiu", "Dah-Ching Ding", "Rong-Kung Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0046504.g007", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_effects_of_human_umbilical_cord_derived_mesenchymal_stem_cells_hUC_MSCs_treatment_on_cleaved_caspase_3_8_and_9_expressions_assessed_by_western_blots_in_the_kidney_tissue_of_NOD_SCID_mice_treated_with_either_folic_acid_or_vehicle_only_A_and_examples_o/239852", "title"=>"The effects of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) treatment on cleaved caspase-3, -8, and -9 expressions (assessed by western blots) in the kidney tissue of NOD-SCID mice treated with either folic acid or vehicle only (A) and examples of fluorescent immunohistochemistry staining for cleaved caspase-3, -8, and -9 in renal tubular cells of NOD-SCID mice treated with FA and subsequently either given or not given hUC-MSCs at day 3 (B).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 22:40:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/569193"], "description"=>"<p>(A) Quantification of PCNA-positive tubular cells. (B) Quantification of TUNEL-positive tubular cells. NOD-SCID: nonobese diabetic-severe combined immunodeficiency disease; PCNA: proliferating cell nuclear antigen; CON: control NOD-SCID mice; FA: NOD-SCID mice were treated with folic acid; FA+hUC-MSCs: NOD-SCID mice were treated with folic acid and then given hUC-MSCs 3 h later; CON+hUC-MSCs: NOD-SCID mice were given hUC-MSCs. Values are means ± SEM. *P<0.05 versus the same group in the pre-folic acid period; ∧P<0.05 versus group CON at the corresponding time point; +P<0.05 versus group CON+hUC-MSCs at the corresponding time point; #P<0.05 versus group FA at the corresponding time point. Additionally, examples of stained TUNEL-positive cells (arrow) were seen in (C) control NOD-SCID mice, (D) NOD-SCID mice were treated with folic acid, (E) NOD-SCID mice were treated with folic acid and then given hUC-MSCs 3 h later, and (F) NOD-SCID mice were given hUC-MSCs.</p>", "links"=>[], "tags"=>["umbilical", "cord-derived", "mesenchymal", "cells", "tubular", "proliferation", "apoptosis", "nod-scid", "mice", "treated", "folic"], "article_id"=>239674, "categories"=>["Cell Biology", "Marine Biology", "Immunology"], "users"=>["Te-Chao Fang", "Cheng-Yoong Pang", "Sheng-Chun Chiu", "Dah-Ching Ding", "Rong-Kung Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0046504.g006", "stats"=>{"downloads"=>0, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_human_umbilical_cord_derived_mesenchymal_stem_cells_hUC_MSCs_on_tubular_cell_proliferation_and_apoptosis_in_NOD_SCID_mice_treated_with_folic_acid_/239674", "title"=>"Effects of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) on tubular cell proliferation and apoptosis in NOD-SCID mice treated with folic acid.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 22:39:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/568386"], "description"=>"<p>(A) The levels of serum urea nitrogen and (B) the levels of serum creatinine. NOD-SCID: nonobese diabetic-severe combined immunodeficiency disease; CON: control NOD-SCID mice; FA: NOD-SCID mice were treated with folic acid; FA+hUC-MSCs: NOD-SCID mice were treated with folic acid and then given hUC-MSCs 3 h later; CON+hUC-MSCs: NOD-SCID mice were treated with hUC-MSCs. Values are means ± SEM. *P<0.05 versus the same group at pre-folic acid period; ∧P<0.05 versus group CON at the corresponding time point; +P<0.05 versus group CON+hUC-MSCs at the corresponding time point; #P<0.05 versus group FA at the corresponding time points. Additionally, renal tubular cells were normal in the control period (C) and in control NOD-SCID mice with hUC-MSCs treatment throughout the experiment (D). Three days after folic acid, renal tubular cell damage was severe in folic acid-injured NOD-SCID mice (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046504#pone-0046504-g002\" target=\"_blank\">Figure 2E</a>) and administration of hUC-MSCs to folic acid-injured NOD-SCID mice could decrease renal tubular cell damage (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046504#pone-0046504-g002\" target=\"_blank\">Figure 2F</a>). The number of inflammatory cells in renal tubulointerstitial area per high power field was shown (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046504#pone-0046504-g002\" target=\"_blank\">Figure 2G</a>). Black arrows indicate where some renal tubular epithelial cells were detached from the basement membrane (cell debris) into the tubular lumen, and white arrows indicate inflammatory cells in the renal tubulointerstitial area. Original magnifications ×100.</p>", "links"=>[], "tags"=>["umbilical", "cord-derived", "mesenchymal", "cells", "transplantation", "renal", "histology", "nod-scid", "mice", "treated", "folic"], "article_id"=>238869, "categories"=>["Cell Biology", "Marine Biology", "Immunology"], "users"=>["Te-Chao Fang", "Cheng-Yoong Pang", "Sheng-Chun Chiu", "Dah-Ching Ding", "Rong-Kung Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0046504.g002", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_effects_of_human_umbilical_cord_derived_mesenchymal_stem_cells_hUC_MSCs_transplantation_on_renal_function_A_and_B_and_renal_histology_C_to_G_of_NOD_SCID_mice_treated_with_either_folic_acid_or_vehicle_only_/238869", "title"=>"The effects of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) transplantation on renal function (A and B) and renal histology (C to G) of NOD-SCID mice treated with either folic acid or vehicle only.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 22:35:23"}
  • {"files"=>["https://ndownloader.figshare.com/files/569010"], "description"=>"<p>(A) Layout of the human cytokine array (RayBio human inflammation antibody array 3). Representative array readouts of human cytokines in the kidney tissue of (B) control NOD-SCID mice, (C) FA-injured NOD-SCID mice, (D) NOD-SCID mice were treated with folic acid and then given hUC-MSCs, and (E) NOD-SCID mice were given hUC-MSCs. The samples of renal tissue from each group were the pool of kidneys from six mice per group. The 40 human inflammation cytokines examined included B-lymphocyte chemoattractant (BLC), eotaxin, eotaxin-2, granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), I-309, inter-cellular adhesion molecule (ICAM)-1, interferon (IFN)γ, interleukin (IL)-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-6 sR, IL-7, IL-8, IL-10, IL-11, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17, monocyte chemotactic protein (MCP-1), macrophage colony-stimulating factor (M-CSF), monokine induced by IFN-Gamma (MIG), macrophage inflammatory protein (MIP)-1α, MIP-1β, MIP-1δ, platelet-derived growth factor B-chain homodimer (PDGF-BB), RANTES (regulated on activation, normal T-cell expressed and secreted), tissue inhibitor of metalloproteinase (TIMP)-1, TIMP-2, tumor necrosis factor (TNF)α, TNFβ, TNF soluble Receptor I (TNF sRI), and TNF soluble Receptor II (TNF sRII). NOD-SCID: nonobese diabetic-severe combined immunodeficiency disease.</p>", "links"=>[], "tags"=>["umbilical", "cord-derived", "mesenchymal", "cells", "transplantation", "cytokine", "kidney", "nod-scid", "mice", "treated", "folic"], "article_id"=>239490, "categories"=>["Cell Biology", "Marine Biology", "Immunology"], "users"=>["Te-Chao Fang", "Cheng-Yoong Pang", "Sheng-Chun Chiu", "Dah-Ching Ding", "Rong-Kung Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0046504.g005", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_effects_of_human_umbilical_cord_derived_mesenchymal_stem_cells_hUC_MSCs_transplantation_on_cytokine_expression_detected_by_a_human_cytokine_array_in_the_kidney_tissue_of_NOD_SCID_mice_treated_with_either_folic_acid_FA_or_vehicle_only_at_day_3_post_tr/239490", "title"=>"The effects of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) transplantation on cytokine expression, detected by a human cytokine array in the kidney tissue of NOD-SCID mice treated with either folic acid (FA) or vehicle only at day 3 post-treatment.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 22:38:42"}
  • {"files"=>["https://ndownloader.figshare.com/files/568598"], "description"=>"<p>(A) human renal tissue (positive control for Cy3-labeled human pan-centromeric paint), (B) is a four-fold magnification of the yellow boxed area in A, (C) human renal tissue (negative control for Cy3-labeled human pan-centromeric paint), (D) normal NOD-SCID mice, (E) is a four-fold magnification of the white boxed area in D, (F) FA-injured NOD-SCID mice, (G) NOD-SCID mice were treated with folic acid and then given hUC-MSCs, (H) NOD-SCID mice were given hUC-MSCs. PHA-L stained renal tubular cells were stained as red color in the luminal side and cytoplasm. Direct FISH was performed using a Cy3-labeled human pan-centromeric paint (multiple red nuclear signals) and a FITC-labeled mouse Y-chromosome paint (a green nuclear signal) together in the same renal sections. DAPI identified nuclei and was seen as a blue signal. No signals of human pan-centromeric paint were observed in any PHA-L-stained cells among the four groups throughout the experiment. PHA-L: <i>Phaseolus vulgaris</i> leucoagglutinin; NOD-SCID: nonobese diabetic-severe combined immunodeficiency disease; DAPI: 4,6-diamidino-2-phenylindole. Original magnifications ×1000.</p>", "links"=>[], "tags"=>["fluorescence", "hybridization", "transdifferentiation", "fusion", "huc-mscs", "pha-l-stained", "renal", "tubular", "cells", "folic", "nod-scid", "mice", "cord-derived", "mesenchymal"], "article_id"=>239069, "categories"=>["Cell Biology", "Marine Biology", "Immunology"], "users"=>["Te-Chao Fang", "Cheng-Yoong Pang", "Sheng-Chun Chiu", "Dah-Ching Ding", "Rong-Kung Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0046504.g003", "stats"=>{"downloads"=>1, "page_views"=>29, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Examples_of_direct_fluorescence_in_situ_hybridization_FISH_examination_to_detect_transdifferentiation_or_fusion_of_hUC_MSCs_in_PHA_L_stained_renal_tubular_cells_of_folic_acid_FA_treated_NOD_SCID_mice_with_and_without_human_cord_derived_mesenchymal_stem_c/239069", "title"=>"Examples of direct fluorescence <i>in situ</i> hybridization (FISH) examination to detect transdifferentiation or fusion of hUC-MSCs in PHA-L-stained renal tubular cells of folic acid (FA)-treated NOD-SCID mice with and without human cord-derived mesenchymal stem cells (hUC-MSCs).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 22:36:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/568207"], "description"=>"<p>(A) The levels of serum urea nitrogen and (B) the levels of serum creatinine. NOD-SCID: nonobese diabetic-severe combined immunodeficiency disease; CON: control NOD-SCID mice; FA: NOD-SCID mice were treated with folic acid; FA+hUC-MSCs 3 hr: NOD-SCID mice suffering from folic acid and then given hUC-MSCs 3 hr later; FA+hUC-MSCs 12 hr: NOD-SCID mice suffering from folic acid and then given hUC-MSCs 12 hr later. Values are means ± SEM. *P<0.05 versus the same group at pre-folic acid period; ∧P<0.05 versus group CON at the corresponding time point; +P<0.05 versus group FA+hUC-MSCs 3 hr at the corresponding time point; #P<0.05 versus group FA+hUC-MSCs 12 hr at the corresponding time points. Additionally, (C) renal tubular cells were normal in the control period and (D) renal tubular cells were still unchanged at the time point of 3 hrs after folic acid administration. (E) Renal tubular cell damage appeared in folic acid-injured NOD-SCID mice at the time point of 24 hours after folic acid. (F) Early and (G) late administrations of hUC-MSCs to NOD-SCID mice suffering from folic acid could decrease renal tubular cell damage. Original magnifications ×400. (H) An example of engraftment hUC-MSCs (FITC-human chromosome X, green color) was observed in the renal tubular cells (<i>Phaseolus vulgaris</i> leucoagglutinin stained, red color) was seen. Original magnifications ×1000. (I) The engraftment of hUC-MSCs in the renal tubular cells was negligible at 24 hr and day 3 in folic acid-injured mice treated with early and late administrations of hUC-MSCs. Additionally, no engraftment of hUC-MSCs in the renal tubular cells could be detected at day 7.</p>", "links"=>[], "tags"=>["administrations", "huc-mscs", "renal", "1a", "histology", "changes", "1c", "nod-scid", "mice", "folic", "acid-induced"], "article_id"=>238684, "categories"=>["Cell Biology", "Marine Biology", "Immunology"], "users"=>["Te-Chao Fang", "Cheng-Yoong Pang", "Sheng-Chun Chiu", "Dah-Ching Ding", "Rong-Kung Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0046504.g001", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_effects_of_early_and_late_administrations_of_hUC_MSCs_on_renal_function_Figure_1A_and_1B_and_renal_histology_changes_Figure_1C_to_F_igure_1I_of_NOD_SCID_mice_suffering_from_folic_acid_induced_AKI_/238684", "title"=>"The effects of early and late administrations of hUC-MSCs on renal function (<b>Figure 1A and 1B</b>) and renal histology changes (<b>Figure 1C to F</b>igure 1I) of NOD-SCID mice suffering from folic acid-induced AKI.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 22:34:17"}
  • {"files"=>["https://ndownloader.figshare.com/files/569676"], "description"=>"<p>CON: control; FA: folic acid; hUC-MSCs: human umbilical cord-derived mesenchymal stem cells. Values are the means ± SEM.</p>*<p>P<0.05 versus the same group in the pre-folic acid period;</p>∧<p>P<0.05 versus group CON at the corresponding time point;</p>+<p>P<0.05 versus group CON+hUC-MSCs at the corresponding time point;</p>#<p>P<0.05 versus group FA at the corresponding time point.</p>", "links"=>[], "tags"=>["folic", "cord-derived", "mesenchymal", "cells", "renal", "tubular", "scores", "nod-scid"], "article_id"=>240162, "categories"=>["Cell Biology", "Marine Biology", "Immunology"], "users"=>["Te-Chao Fang", "Cheng-Yoong Pang", "Sheng-Chun Chiu", "Dah-Ching Ding", "Rong-Kung Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0046504.t001", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_administration_of_folic_acid_alone_and_in_combination_with_human_cord_derived_mesenchymal_stem_cells_on_renal_tubular_injury_scores_in_male_NOD_SCID_mice_/240162", "title"=>"Effects of administration of folic acid alone and in combination with human cord-derived mesenchymal stem cells on renal tubular injury scores in male NOD-SCID mice.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-19 22:42:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/568823"], "description"=>"<p>(A) Layout of the mouse cytokine array (RayBio mouse inflammation antibody array 1). Representative array readouts of mouse cytokine in the kidney tissue of (B) control NOD-SCID mice, (C) FA-injured NOD-SCID mice, (D) NOD-SCID mice were treated with folic acid and then given hUC-MSCs, and (E) NOD-SCID mice were given hUC-MSCs. The samples of renal tissue from each group were the pool of kidneys from six mice per group. The 40 mouse inflammation cytokines examined included B-lymphocyte chemoattractant (BLC), CD30 Ligand (CD 30 L), eotaxin, eotaxin-2, Fas Ligand, fractalkine, granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon (IFN)γ, interleukin (IL)-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-6, IL-9, IL-10, IL-12p40p70, IL-12p70, IL-13, IL-17, inducible T cell alpha chemoattractant (I-TAC), keratinocyte-derived cytokine (KC), leptin, lipopolysaccharide-induced CXC chemokine (LIX), lymphotactin, monocyte chemotactic protein (MCP-1), macrophage colony-stimulating factor (M-CSF), monokine induced by IFN-Gamma (MIG), macrophage inflammatory protein (MIP)-1α, MIP-1γ, RANTES (regulated on activation, normal T-cell expressed and secreted), stromal cell-derived factor-1 (SDF-1), T-cell activation protein 3 (TCA-3), thymus-expressed chemokine (TECK), tissue inhibitor of metalloproteinase (TIMP)-1, TIMP-2, tumor necrosis factor (TNF)α, soluble TNF Receptor I (sTNF RI), and soluble TNF Receptor II (sTNF RII). NOD-SCID: nonobese diabetic-severe combined immunodeficiency disease.</p>", "links"=>[], "tags"=>["umbilical", "cord-derived", "mesenchymal", "cells", "transplantation", "cytokine", "kidney", "nod-scid", "mice", "treated", "folic"], "article_id"=>239306, "categories"=>["Cell Biology", "Marine Biology", "Immunology"], "users"=>["Te-Chao Fang", "Cheng-Yoong Pang", "Sheng-Chun Chiu", "Dah-Ching Ding", "Rong-Kung Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0046504.g004", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_effects_of_human_umbilical_cord_derived_mesenchymal_stem_cells_hUC_MSCs_transplantation_on_cytokine_expression_detected_by_a_mouse_cytokine_array_in_the_kidney_tissue_of_NOD_SCID_mice_treated_with_either_folic_acid_FA_or_vehicle_only_at_day_3_post_tr/239306", "title"=>"The effects of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) transplantation on cytokine expression, detected by a mouse cytokine array in the kidney tissue of NOD-SCID mice treated with either folic acid (FA) or vehicle only, at day 3 post-treatment.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 22:37:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/569542"], "description"=>"<p>HEK293, human embryonic kidney 293 cell line; hUC-MSCs, human umbilical cord-derived mesenchymal stem cells; IGF1, insulin-like growth factor 1. Additionally, IGF1 stimulated IGFR and then induced Akt phosphorylation in kidney cells of NOD-SCID mice which were suffering from folic acid and then given hUC-MSCs (B). At different time points, whole kidney extracts (made from the pool of kidneys from six animals per group) were used for Western blot analysis of IGFR, phsopho-Akt (Ser473-Akt), toal Akt, and β-actin (as internal control). The examples of phosphor-Akt expression in this kidney section were shown (C). IGFR, insulin-like growth factor 1 receptor; CON, control NOD-SCID mice; FA, NOD-SCID mice were treated with folic acid; FA+hUC-MSCs, NOD-SCID mice were treated with folic acid and then given hUC-MSCs 3 h later; CON+hUC-MSCs, NOD-SCID mice were given hUC-MSCs; MW, molecular weight; kDa, kilodalton.</p>", "links"=>[], "tags"=>["blot", "demonstrated", "igf1", "huc-mscs"], "article_id"=>240033, "categories"=>["Cell Biology", "Marine Biology", "Immunology"], "users"=>["Te-Chao Fang", "Cheng-Yoong Pang", "Sheng-Chun Chiu", "Dah-Ching Ding", "Rong-Kung Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0046504.g008", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Western_blot_demonstrated_the_expression_of_IGF1_in_the_hUC_MSCs_A_/240033", "title"=>"Western blot demonstrated the expression of IGF1 in the hUC-MSCs (A).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 22:41:50"}

PMC Usage Stats | Further Information

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Relative Metric

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