Fluorescence2D: Software for Accelerated Acquisition and Analysis of Two-Dimensional Fluorescence Spectra
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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1576184"], "description"=>"<p>(Panel <b>A</b>) The 2D spectrum of the buffer solution (without baseline subtraction) is shown contoured between 0 and 10% of total intensity (blue to red). The first order reflection, <b>1</b>; the second order reflection, <b>2</b>; Raman scattering, <b>3</b>. The black solid line separates the lower area with experimental data from the upper area added to make the dataset rectangular for visualization. (Panel <b>B</b>) The 2D spectrum of H-Ras in the complex with mant-GDP. The spectrum of the buffer solution (from Panel <b>A</b>) was subtracted as a baseline; the resulting graph was contoured from 3 to 13% of total intensity scale. Tyrosine fluorescence, <b>4</b>; its second order reflection, <b>5</b>; mant-GDP emission, <b>6</b> and <b>7</b>. Spectra in both panels were “zoomed in” using interactive MATLAB graphics tools. The original and processed experimental data along with the corresponding <i>fluorescence2Dmain.m</i> script are included with the <i>Fluorescence2D</i> package in the <i>sample_data/</i>folder.</p>", "links"=>[], "tags"=>["molecular biology", "Molecular biology techniques", "Molecular biology assays and analysis techniques", "Fluorescence-assisted mismatch analysis", "physics", "Electromagnetic radiation", "luminescence", "fluorescence", "Bioassays and physiological analysis", "Spectrum analysis techniques", "spectrophotometry", "fluorimetry", "fluorophotometry", "Fluorescence spectroscopy", "spectra", "h-ras"], "article_id"=>1090112, "categories"=>["Biological Sciences"], "users"=>["Evgenii L. Kovrigin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0101227.g004", "stats"=>{"downloads"=>3, "page_views"=>31, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Two_dimensional_spectra_of_H_Ras_in_the_complex_with_mant_GDP_/1090112", "title"=>"Two-dimensional spectra of H-Ras in the complex with mant-GDP.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-07-01 03:06:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/1576185"], "description"=>"<p>The 1D spectra were extracted from the full 2D spectrum at the excitation wavelength of 360(<b>A</b>), and at the emission wavelength of 440 nm (<b>B</b>), respectively.</p>", "links"=>[], "tags"=>["molecular biology", "Molecular biology techniques", "Molecular biology assays and analysis techniques", "Fluorescence-assisted mismatch analysis", "physics", "Electromagnetic radiation", "luminescence", "fluorescence", "Bioassays and physiological analysis", "Spectrum analysis techniques", "spectrophotometry", "fluorimetry", "fluorophotometry", "Fluorescence spectroscopy", "slices", "2d", "extracted", "specified", "excitation"], "article_id"=>1090113, "categories"=>["Biological Sciences"], "users"=>["Evgenii L. Kovrigin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0101227.g005", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_One_dimensional_slices_of_the_2D_spectrum_extracted_at_specified_emission_and_excitation_wavelengths_/1090113", "title"=>"One-dimensional slices of the 2D spectrum extracted at specified emission and excitation wavelengths.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-07-01 03:06:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/1576179"], "description"=>"<p>(<b>A</b>) Example of a conventional array of excitation/emission wavelengths used for acquisition of two-dimensional fluorescence spectra. The diagonal (λ<sub>em</sub> = λ<sub>ex</sub>) is shown with the open symbols. The conventional 1D emission spectrum is a horizontal set of points for each excitation wavelength to the right of the open symbol. Correspondingly, the 1D excitation spectrum is a vertical set of points below the open symbol. (<b>B</b>) The triangular array of excitation/emission wavelengths where the emission ranges always begin at a greater wavelength than the excitation wavelength in each row. The specific 100 nm increment in this figure is chosen solely for clarity of presentation.</p>", "links"=>[], "tags"=>["molecular biology", "Molecular biology techniques", "Molecular biology assays and analysis techniques", "Fluorescence-assisted mismatch analysis", "physics", "Electromagnetic radiation", "luminescence", "fluorescence", "Bioassays and physiological analysis", "Spectrum analysis techniques", "spectrophotometry", "fluorimetry", "fluorophotometry", "Fluorescence spectroscopy", "triangular", "two-dimensional"], "article_id"=>1090107, "categories"=>["Biological Sciences"], "users"=>["Evgenii L. Kovrigin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0101227.g001", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Rectangular_and_triangular_two_dimensional_spectra_/1090107", "title"=>"Rectangular and triangular two-dimensional spectra.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-07-01 03:06:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/1576181"], "description"=>"<p>A typical workflow of the two-dimensional acquisition and data analysis.</p>", "links"=>[], "tags"=>["molecular biology", "Molecular biology techniques", "Molecular biology assays and analysis techniques", "Fluorescence-assisted mismatch analysis", "physics", "Electromagnetic radiation", "luminescence", "fluorescence", "Bioassays and physiological analysis", "Spectrum analysis techniques", "spectrophotometry", "fluorimetry", "fluorophotometry", "Fluorescence spectroscopy", "workflow", "two-dimensional"], "article_id"=>1090109, "categories"=>["Biological Sciences"], "users"=>["Evgenii L. Kovrigin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0101227.g002", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_A_typical_workflow_of_the_two_dimensional_acquisition_and_data_analysis_/1090109", "title"=>"A typical workflow of the two-dimensional acquisition and data analysis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-07-01 03:06:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/1576182"], "description"=>"<p>The interactive tools of MATLAB allow for easy adjustment, inspection, and export of the graphics created by <i>Fluorescence2D</i>. In the panel <b>A</b>, arrows indicate locations of the zoom tool (<b>1</b>) used to enlarge the desired area, the data inspector tool (<b>2</b>) allowing to determine values of wavelength and intensity at any specific point, and the interactive graphics mode toggle (<b>3</b>) for adjustment of font sizes, labels, ticks, and colors. Export of the final image into a desired format is performed through <i>File:Save As</i> menu item as shown in Panel <b>B</b>.</p>", "links"=>[], "tags"=>["molecular biology", "Molecular biology techniques", "Molecular biology assays and analysis techniques", "Fluorescence-assisted mismatch analysis", "physics", "Electromagnetic radiation", "luminescence", "fluorescence", "Bioassays and physiological analysis", "Spectrum analysis techniques", "spectrophotometry", "fluorimetry", "fluorophotometry", "Fluorescence spectroscopy", "graphic"], "article_id"=>1090110, "categories"=>["Biological Sciences"], "users"=>["Evgenii L. Kovrigin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0101227.g003", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Manipulation_of_the_graphic_output_of_Fluorescence2D_/1090110", "title"=>"Manipulation of the graphic output of <i>Fluorescence2D</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-07-01 03:06:18"}

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Relative Metric

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