Pseudomonas aeruginosa Biofilm Formation and Persistence, along with the Production of Quorum Sensing-Dependent Virulence Factors, Are Disrupted by a Triterpenoid Coumarate Ester Isolated from Dalbergia trichocarpa, a Tropical Legume
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{"title"=>"Pseudomonas aeruginosa biofilm formation and persistence, along with the production of quorum sensing-dependent virulence factors, are disrupted by a triterpenoid coumarate ester isolated from dalbergia trichocarpa, a tropical legume", "type"=>"journal", "authors"=>[{"first_name"=>"Tsiry", "last_name"=>"Rasamiravaka", "scopus_author_id"=>"24332857700"}, {"first_name"=>"Olivier M.", "last_name"=>"Vandeputte", "scopus_author_id"=>"15926298700"}, {"first_name"=>"Laurent", "last_name"=>"Pottier", "scopus_author_id"=>"26432741000"}, {"first_name"=>"Joelle", "last_name"=>"Huet", "scopus_author_id"=>"12039400900"}, {"first_name"=>"Christian", "last_name"=>"Rabemanantsoa", "scopus_author_id"=>"25636370500"}, {"first_name"=>"Martin", "last_name"=>"Kiendrebeogo", "scopus_author_id"=>"8872004700"}, {"first_name"=>"Abel", "last_name"=>"Andriantsimahavandy", "scopus_author_id"=>"6701554634"}, {"first_name"=>"Andry", "last_name"=>"Rasamindrakotroka", "scopus_author_id"=>"6603635308"}, {"first_name"=>"Caroline", "last_name"=>"Stévigny", "scopus_author_id"=>"6507302831"}, {"first_name"=>"Pierre", "last_name"=>"Duez", "scopus_author_id"=>"6602127834"}, {"first_name"=>"Mondher", "last_name"=>"El Jaziri", "scopus_author_id"=>"56269953000"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84940826845", "pmid"=>"26186595", "sgr"=>"84940826845", "isbn"=>"1932-6203", "pui"=>"605870664", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0132791"}, "id"=>"d0aa7498-6a4c-3bfe-8f08-7e4183299bfc", "abstract"=>"Recently, extracts of Dalbergia trichocarpa bark have been shown to disrupt P. aeruginosa PAO1 quorum sensing (QS) mechanisms, which are key regulators of virulence factor expression and implicated in biofilm formation. One of the active compounds has been isolated and identified as oleanolic aldehyde coumarate (OALC), a novel bioactive compound that inhibits the formation of P. aeruginosa PAO1 biofilm and its maintenance as well as the expression of the las and rhl QS systems. Consequently, the production of QS-controlled virulence factors including, rhamnolipids, pyocyanin, elastase and extracellular polysaccharides as well as twitching and swarming motilities is reduced. Native acylhomoserine lactones (AHLs) production is inhibited by OALC but exogenous supply of AHLs does not restore the production of virulence factors by OALC-treated cultures, indicating that OALC exerts its effect beyond AHLs synthesis in the QS pathways. Further experiments provided a significant inhibition of the global virulence factor activator gacA by OALC. OALC disorganizes established biofilm structure and improves the bactericidal activity of tobramycin against biofilm-encapsulated PAO1 cells. Finally, a significant reduction of Caenorhabditis elegans paralysis was recorded when the worms were infected with OALC-pre-treated P. aeruginosa. Taken together, these results show that triterpenoid coumarate esters are suitable chemical backbones to target P. aeruginosa virulence mechanisms.", "link"=>"http://www.mendeley.com/research/pseudomonas-aeruginosa-biofilm-formation-persistence-along-production-quorum-sensingdependent-virule", "reader_count"=>34, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Student > Doctoral Student"=>1, "Researcher"=>6, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>2, "Student > Master"=>7, "Other"=>1, "Student > Bachelor"=>7, "Lecturer"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Student > Doctoral Student"=>1, "Researcher"=>6, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>2, "Student > Master"=>7, "Other"=>1, "Student > Bachelor"=>7, "Lecturer"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Environmental Science"=>2, "Biochemistry, Genetics and Molecular Biology"=>2, "Nursing and Health Professions"=>1, "Agricultural and Biological Sciences"=>18, "Medicine and Dentistry"=>5, "Design"=>1, "Chemistry"=>1, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Design"=>{"Design"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>5}, "Chemistry"=>{"Chemistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>18}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>2}, "Environmental Science"=>{"Environmental Science"=>2}}, "reader_count_by_country"=>{"Canada"=>1, "Romania"=>1, "Turkey"=>1, "Belgium"=>2}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2180079"], "description"=>"<p>(A) Swarming motility of <i>P</i>. <i>aeruginosa</i> PAO1 onto LB agar (0.6%) supplemented with glutamate (0.05%), glucose (0.2%) and DMSO (1%) or OALC (200μM final concentration), naringenin (Nar, 4 mM final concentration) or naringin (Nin, 4 mM final concentration). After incubation at 37°C for 24 hours, the zones of migration (down) from the point of inoculation were measured (up) for each condition. (B) Twitching motility of <i>P</i>. <i>aeruginosa</i> PAO1 onto LB agar (1%) supplemented with DMSO (1%), OALC (200μM final concentration), Nar (4 mM final concentration) or Nin (4 mM final concentration). The twitching zones were stained (down) and their diameters (up) measured after incubation at 37°C for 48 h. Error bars represent the standard errors of the means and all experiments were performed in quintuplicate with three independent assays and asterisks indicate samples that are significantly different from the DMSO (Student’s <i>t</i>-tests; <i>P</i> ≤ 0.01).</p>", "links"=>[], "tags"=>["results show", "triterpenoid coumarate esters", "Native acylhomoserine lactones", "extracellular polysaccharides", "rhl QS systems", "bactericidal activity", "virulence factor activator gacA", "Virulence factors", "biofilm formation", "virulence factor expression", "target P", "aeruginosa PAO 1 biofilm", "OALC disorganizes", "Tropical Legume", "Caenorhabditis elegans paralysis", "Dalbergia trichocarpa bark", "QS pathways", "exogenous supply", "chemical backbones", "Pseudomonas aeruginosa Biofilm Formation", "Triterpenoid Coumarate Ester", "aeruginosa virulence mechanisms", "oleanolic aldehyde coumarate", "biofilm structure", "aeruginosa PAO 1 quorum", "AHLs synthesis", "Dalbergia trichocarpa", "novel bioactive compound"], "article_id"=>1486560, "categories"=>["Biological Sciences", "Ecology"], "users"=>["Tsiry Rasamiravaka", "Olivier M. Vandeputte", "Laurent Pottier", "Joëlle Huet", "Christian Rabemanantsoa", "Martin Kiendrebeogo", "Abel Andriantsimahavandy", "Andry Rasamindrakotroka", "Caroline Stévigny", "Pierre Duez", "Mondher El Jaziri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0132791.g006", "stats"=>{"downloads"=>1, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_OALC_on_P_aeruginosa_PAO1_motilities_/1486560", "title"=>"Effect of OALC on <i>P</i>. <i>aeruginosa</i> PAO1 motilities.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-17 02:51:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/2180060"], "description"=>"<p>Chemical structure of the bioactive compound assigned as 3β-hydroxyolean-12-en-28-al 3-<i>p</i>-coumarate (oleanolic aldehyde coumarate, OALC).</p>", "links"=>[], "tags"=>["results show", "triterpenoid coumarate esters", "Native acylhomoserine lactones", "extracellular polysaccharides", "rhl QS systems", "bactericidal activity", "virulence factor activator gacA", "Virulence factors", "biofilm formation", "virulence factor expression", "target P", "aeruginosa PAO 1 biofilm", "OALC disorganizes", "Tropical Legume", "Caenorhabditis elegans paralysis", "Dalbergia trichocarpa bark", "QS pathways", "exogenous supply", "chemical backbones", "Pseudomonas aeruginosa Biofilm Formation", "Triterpenoid Coumarate Ester", "aeruginosa virulence mechanisms", "oleanolic aldehyde coumarate", "biofilm structure", "aeruginosa PAO 1 quorum", "AHLs synthesis", "Dalbergia trichocarpa", "novel bioactive compound"], "article_id"=>1486541, "categories"=>["Biological Sciences", "Ecology"], "users"=>["Tsiry Rasamiravaka", "Olivier M. Vandeputte", "Laurent Pottier", "Joëlle Huet", "Christian Rabemanantsoa", "Martin Kiendrebeogo", "Abel Andriantsimahavandy", "Andry Rasamindrakotroka", "Caroline Stévigny", "Pierre Duez", "Mondher El Jaziri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0132791.g001", "stats"=>{"downloads"=>3, "page_views"=>71, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Chemical_structure_of_the_bioactive_compound_assigned_as_3_hydroxyolean_12_en_28_al_3_p_coumarate_oleanolic_aldehyde_coumarate_OALC_/1486541", "title"=>"Chemical structure of the bioactive compound assigned as 3β-hydroxyolean-12-en-28-al 3-<i>p</i>-coumarate (oleanolic aldehyde coumarate, OALC).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-17 02:51:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/2180086"], "description"=>"<p>PAO1 cells were incubated statically for 24 hours in the presence of DMSO, naringin (4 mM), naringenin (4 mM) or OALC (200 μM) and then treated for 24 hours with tobramycin (100 μg mL<sup>-1</sup>). (A) OALC + tobramycin, (B) tobramycin, (C) naringenin + tobramycin, (D) DMSO + tobramycin, (E) naringin + tobramycin. Assessment of bacterial viability and microscopy were performed as in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132791#pone.0132791.g006\" target=\"_blank\">Fig 6</a>. (F) Quantification of bacterial viability. Error bars represent the standard errors of the means; all experiments were performed in quintuplicate with three independent assays and asterisks indicate samples that are significantly different from the DMSO (Student’s <i>t</i>-tests; <i>P</i> ≤ 0.01).</p>", "links"=>[], "tags"=>["results show", "triterpenoid coumarate esters", "Native acylhomoserine lactones", "extracellular polysaccharides", "rhl QS systems", "bactericidal activity", "virulence factor activator gacA", "Virulence factors", "biofilm formation", "virulence factor expression", "target P", "aeruginosa PAO 1 biofilm", "OALC disorganizes", "Tropical Legume", "Caenorhabditis elegans paralysis", "Dalbergia trichocarpa bark", "QS pathways", "exogenous supply", "chemical backbones", "Pseudomonas aeruginosa Biofilm Formation", "Triterpenoid Coumarate Ester", "aeruginosa virulence mechanisms", "oleanolic aldehyde coumarate", "biofilm structure", "aeruginosa PAO 1 quorum", "AHLs synthesis", "Dalbergia trichocarpa", "novel bioactive compound"], "article_id"=>1486567, "categories"=>["Biological Sciences", "Ecology"], "users"=>["Tsiry Rasamiravaka", "Olivier M. Vandeputte", "Laurent Pottier", "Joëlle Huet", "Christian Rabemanantsoa", "Martin Kiendrebeogo", "Abel Andriantsimahavandy", "Andry Rasamindrakotroka", "Caroline Stévigny", "Pierre Duez", "Mondher El Jaziri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0132791.g008", "stats"=>{"downloads"=>0, "page_views"=>23, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Synergistic_activity_of_OALC_with_tobramycin_against_biofilm_encapsulated_P_aeruginosa_PAO1_/1486567", "title"=>"Synergistic activity of OALC with tobramycin against biofilm-encapsulated <i>P</i>. <i>aeruginosa</i> PAO1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-17 02:51:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/2180068"], "description"=>"<p>(A) Quantification of biofilm formation by <i>P</i>. <i>aeruginosa</i> PAO1 grown in minimal (grey bar) and complex media (clear bar) after static incubation at 37°C for 24 hours. (B) Quantification of biofilm formation by <i>P</i>. <i>aeruginosa</i> grown in in minimal media (grey bar) and C.F.U. measurement (clear bar) of planktonic bacteria after static incubation at 37°C for 24 hours. Biofilm formation was quantified by crystal violet staining and measured at A<sub>590nm</sub>. The cell density of the bacteria was assessed at 600 nm. Error bars represent the standard errors of the means; all experiments were performed in quintuplicate with three independent assays and asterisks indicate samples that are significantly different from the DMSO (Student’s <i>t</i>-tests; <i>P</i> ≤ 0.01).</p>", "links"=>[], "tags"=>["results show", "triterpenoid coumarate esters", "Native acylhomoserine lactones", "extracellular polysaccharides", "rhl QS systems", "bactericidal activity", "virulence factor activator gacA", "Virulence factors", "biofilm formation", "virulence factor expression", "target P", "aeruginosa PAO 1 biofilm", "OALC disorganizes", "Tropical Legume", "Caenorhabditis elegans paralysis", "Dalbergia trichocarpa bark", "QS pathways", "exogenous supply", "chemical backbones", "Pseudomonas aeruginosa Biofilm Formation", "Triterpenoid Coumarate Ester", "aeruginosa virulence mechanisms", "oleanolic aldehyde coumarate", "biofilm structure", "aeruginosa PAO 1 quorum", "AHLs synthesis", "Dalbergia trichocarpa", "novel bioactive compound"], "article_id"=>1486549, "categories"=>["Biological Sciences", "Ecology"], "users"=>["Tsiry Rasamiravaka", "Olivier M. Vandeputte", "Laurent Pottier", "Joëlle Huet", "Christian Rabemanantsoa", "Martin Kiendrebeogo", "Abel Andriantsimahavandy", "Andry Rasamindrakotroka", "Caroline Stévigny", "Pierre Duez", "Mondher El Jaziri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0132791.g004", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_OALC_on_biofilm_formation_by_P_aeruginosa_/1486549", "title"=>"Effect of OALC on biofilm formation by <i>P</i>. <i>aeruginosa</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-17 02:51:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/2180067"], "description"=>"<p>(A) Effect of OALC on <i>lasR</i> (grey bar) and <i>lasI</i> (clear bar) expression following 18 hours of growth. (B) Effect of OALC on <i>rhlR</i> (grey bar) and <i>rhlI</i> (clear bar) expression following 18 hours of growth. (C) Effect of OALC on <i>gacA</i> (grey bar) and <i>vfr</i> (clear bar) expression following 18 hours of growth. Gene expression was measured as the β-galactosidase activity of the <i>lacZ</i> gene fusions and expressed in Miller units. Error bars represent the standard errors of the means; all experiments were performed in quintuplicate with three independent assays and asterisks indicate samples that are significantly different from the DMSO (Student’s <i>t</i>-tests; <i>P</i> ≤ 0.01).</p>", "links"=>[], "tags"=>["results show", "triterpenoid coumarate esters", "Native acylhomoserine lactones", "extracellular polysaccharides", "rhl QS systems", "bactericidal activity", "virulence factor activator gacA", "Virulence factors", "biofilm formation", "virulence factor expression", "target P", "aeruginosa PAO 1 biofilm", "OALC disorganizes", "Tropical Legume", "Caenorhabditis elegans paralysis", "Dalbergia trichocarpa bark", "QS pathways", "exogenous supply", "chemical backbones", "Pseudomonas aeruginosa Biofilm Formation", "Triterpenoid Coumarate Ester", "aeruginosa virulence mechanisms", "oleanolic aldehyde coumarate", "biofilm structure", "aeruginosa PAO 1 quorum", "AHLs synthesis", "Dalbergia trichocarpa", "novel bioactive compound"], "article_id"=>1486548, "categories"=>["Biological Sciences", "Ecology"], "users"=>["Tsiry Rasamiravaka", "Olivier M. Vandeputte", "Laurent Pottier", "Joëlle Huet", "Christian Rabemanantsoa", "Martin Kiendrebeogo", "Abel Andriantsimahavandy", "Andry Rasamindrakotroka", "Caroline Stévigny", "Pierre Duez", "Mondher El Jaziri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0132791.g003", "stats"=>{"downloads"=>0, "page_views"=>25, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_OALC_on_QS_genes_lasI_R_and_rhlI_R_and_global_activator_genes_gacA_and_vfr_expression_in_P_aeruginosa_PAO1_/1486548", "title"=>"Effect of OALC on QS genes (<i>lasI/R and rhlI/R</i>) and global activator genes (<i>gacA</i> and <i>vfr</i>) expression in <i>P</i>. <i>aeruginosa</i> PAO1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-17 02:51:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/2180096"], "description"=>"<p>In <i>P</i>. <i>aeruginosa</i>, OALC inhibits <i>gacA</i> gene expression (key component of <i>GacS/GacA/Rsm</i> signal transduction pathway) leading to an increased level of free RsmA [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132791#pone.0132791.ref095\" target=\"_blank\">95</a>]. The latter negatively regulates <i>las</i> and <i>rhl</i> systems leading to inhibition of rhamnolipids, pyocyanin and elastase production [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132791#pone.0132791.ref009\" target=\"_blank\">9</a>] as well as components involved in biofilm formation and maintenance, including motilities (swarming and twitching) and Psl/Pel productions [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132791#pone.0132791.ref107\" target=\"_blank\">107</a>] independently of QS systems. Exopolysaccharides production is also reduced directly by RsmA [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132791#pone.0132791.ref099\" target=\"_blank\">99</a>].</p>", "links"=>[], "tags"=>["results show", "triterpenoid coumarate esters", "Native acylhomoserine lactones", "extracellular polysaccharides", "rhl QS systems", "bactericidal activity", "virulence factor activator gacA", "Virulence factors", "biofilm formation", "virulence factor expression", "target P", "aeruginosa PAO 1 biofilm", "OALC disorganizes", "Tropical Legume", "Caenorhabditis elegans paralysis", "Dalbergia trichocarpa bark", "QS pathways", "exogenous supply", "chemical backbones", "Pseudomonas aeruginosa Biofilm Formation", "Triterpenoid Coumarate Ester", "aeruginosa virulence mechanisms", "oleanolic aldehyde coumarate", "biofilm structure", "aeruginosa PAO 1 quorum", "AHLs synthesis", "Dalbergia trichocarpa", "novel bioactive compound"], "article_id"=>1486577, "categories"=>["Biological Sciences", "Ecology"], "users"=>["Tsiry Rasamiravaka", "Olivier M. Vandeputte", "Laurent Pottier", "Joëlle Huet", "Christian Rabemanantsoa", "Martin Kiendrebeogo", "Abel Andriantsimahavandy", "Andry Rasamindrakotroka", "Caroline Stévigny", "Pierre Duez", "Mondher El Jaziri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0132791.g010", "stats"=>{"downloads"=>0, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Proposed_targets_and_inhibition_cascade_occurring_in_P_aeruginosa_in_presence_of_OALC_/1486577", "title"=>"Proposed targets and inhibition cascade occurring in <i>P</i>. <i>aeruginosa</i> in presence of OALC.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-17 02:51:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/2180081"], "description"=>"<p>(A) Quantification of total extracellular polysaccharides. The cell density of the bacteria was assessed at 600nm and extracellular polysaccharides production was measured using Phenol-Sulfuric Acid (PSA) method and expressed in μg mL<sup>-1</sup> with glucose as standard. (B) Quantification of alginate. The cell density of the bacteria was assessed at 600nm and alginate production was measured using carbazole method and expressed in μg mL<sup>-1</sup> with sodium alginate as standard. Error bars represent the standard errors of the means; all experiments were performed in quintuplicate with three independent assays and asterisks indicate samples that are significantly different from the DMSO (Student’s <i>t</i>-tests; <i>P</i> ≤ 0.01). Naringenin (Nar) and naringin (Nin) both at 4 mM final concentration were used as quorum sensing inhibitor positive and negative controls, respectively.</p>", "links"=>[], "tags"=>["results show", "triterpenoid coumarate esters", "Native acylhomoserine lactones", "extracellular polysaccharides", "rhl QS systems", "bactericidal activity", "virulence factor activator gacA", "Virulence factors", "biofilm formation", "virulence factor expression", "target P", "aeruginosa PAO 1 biofilm", "OALC disorganizes", "Tropical Legume", "Caenorhabditis elegans paralysis", "Dalbergia trichocarpa bark", "QS pathways", "exogenous supply", "chemical backbones", "Pseudomonas aeruginosa Biofilm Formation", "Triterpenoid Coumarate Ester", "aeruginosa virulence mechanisms", "oleanolic aldehyde coumarate", "biofilm structure", "aeruginosa PAO 1 quorum", "AHLs synthesis", "Dalbergia trichocarpa", "novel bioactive compound"], "article_id"=>1486562, "categories"=>["Biological Sciences", "Ecology"], "users"=>["Tsiry Rasamiravaka", "Olivier M. Vandeputte", "Laurent Pottier", "Joëlle Huet", "Christian Rabemanantsoa", "Martin Kiendrebeogo", "Abel Andriantsimahavandy", "Andry Rasamindrakotroka", "Caroline Stévigny", "Pierre Duez", "Mondher El Jaziri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0132791.g007", "stats"=>{"downloads"=>0, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_OALC_on_extracellular_polysaccharides_production_by_P_aeruginosa_PAO1_/1486562", "title"=>"Effect of OALC on extracellular polysaccharides production by <i>P</i>. <i>aeruginosa</i> PAO1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-17 02:51:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/2180093"], "description"=>"<p>PAO1 cells were incubated statically for 24 hours and then treated for 24 hours with tobramycin (100 μg mL<sup>-1</sup>) and DMSO, naringin (4 mM), naringenin (4 mM) or OALC (200 μM final concentration). (A) OALC + tobramycin, (B) tobramycin, (C) naringenin + tobramycin, (D) DMSO + tobramycin, (E) naringin + tobramycin. Assessment of bacterial viability and microscopy were performed as in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132791#pone.0132791.g006\" target=\"_blank\">Fig 6</a>. (F) Quantification of bacterial viability. Error bars represent the standard errors of the means; all experiments were performed in quintuplicate with three independent assays and asterisks indicate samples that are significantly different from the DMSO (Student’s <i>t</i>-tests; <i>P</i> ≤ 0.01).</p>", "links"=>[], "tags"=>["results show", "triterpenoid coumarate esters", "Native acylhomoserine lactones", "extracellular polysaccharides", "rhl QS systems", "bactericidal activity", "virulence factor activator gacA", "Virulence factors", "biofilm formation", "virulence factor expression", "target P", "aeruginosa PAO 1 biofilm", "OALC disorganizes", "Tropical Legume", "Caenorhabditis elegans paralysis", "Dalbergia trichocarpa bark", "QS pathways", "exogenous supply", "chemical backbones", "Pseudomonas aeruginosa Biofilm Formation", "Triterpenoid Coumarate Ester", "aeruginosa virulence mechanisms", "oleanolic aldehyde coumarate", "biofilm structure", "aeruginosa PAO 1 quorum", "AHLs synthesis", "Dalbergia trichocarpa", "novel bioactive compound"], "article_id"=>1486574, "categories"=>["Biological Sciences", "Ecology"], "users"=>["Tsiry Rasamiravaka", "Olivier M. Vandeputte", "Laurent Pottier", "Joëlle Huet", "Christian Rabemanantsoa", "Martin Kiendrebeogo", "Abel Andriantsimahavandy", "Andry Rasamindrakotroka", "Caroline Stévigny", "Pierre Duez", "Mondher El Jaziri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0132791.g009", "stats"=>{"downloads"=>2, "page_views"=>23, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Synergistic_activity_of_OALC_with_tobramycin_against_biofilm_encapsulated_P_aeruginosa_PAO1_/1486574", "title"=>"Synergistic activity of OALC with tobramycin against biofilm-encapsulated <i>P</i>. <i>aeruginosa</i> PAO1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-17 02:51:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/2180075"], "description"=>"<p>(A) Fluorescence microscopy images of PAO1 cells incubated statically at 37°C for 24 hours. Cells were visualized after staining with SYTO-9 (green fluorescence for living bacteria) and propidium iodide (red fluorescence for dead bacteria) furnished in the LIVE/DEAD <i>Bac</i>Light kit. (B) Fluorescence microscopic images of biofilm formation by PAO1 cells incubated for 24 hours and then treated for 24 hours with DMSO, naringin, naringenin or OALC. Fluorescence microscopy was achieved by using a Leica DM IRE2 inverted fluorescence microscope using a 40x objective lens and images were false-colored and assembled using Adobe Photoshop.</p>", "links"=>[], "tags"=>["results show", "triterpenoid coumarate esters", "Native acylhomoserine lactones", "extracellular polysaccharides", "rhl QS systems", "bactericidal activity", "virulence factor activator gacA", "Virulence factors", "biofilm formation", "virulence factor expression", "target P", "aeruginosa PAO 1 biofilm", "OALC disorganizes", "Tropical Legume", "Caenorhabditis elegans paralysis", "Dalbergia trichocarpa bark", "QS pathways", "exogenous supply", "chemical backbones", "Pseudomonas aeruginosa Biofilm Formation", "Triterpenoid Coumarate Ester", "aeruginosa virulence mechanisms", "oleanolic aldehyde coumarate", "biofilm structure", "aeruginosa PAO 1 quorum", "AHLs synthesis", "Dalbergia trichocarpa", "novel bioactive compound"], "article_id"=>1486556, "categories"=>["Biological Sciences", "Ecology"], "users"=>["Tsiry Rasamiravaka", "Olivier M. Vandeputte", "Laurent Pottier", "Joëlle Huet", "Christian Rabemanantsoa", "Martin Kiendrebeogo", "Abel Andriantsimahavandy", "Andry Rasamindrakotroka", "Caroline Stévigny", "Pierre Duez", "Mondher El Jaziri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0132791.g005", "stats"=>{"downloads"=>4, "page_views"=>33, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_PAO1_biofilm_phenotypes_as_affected_by_DMSO_naringin_naringenin_or_OALC_/1486556", "title"=>"PAO1 biofilm phenotypes as affected by DMSO, naringin, naringenin or OALC.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-17 02:51:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/2180104", "https://ndownloader.figshare.com/files/2180105", "https://ndownloader.figshare.com/files/2180106", "https://ndownloader.figshare.com/files/2180107", "https://ndownloader.figshare.com/files/2180108", "https://ndownloader.figshare.com/files/2180109", "https://ndownloader.figshare.com/files/2180110", "https://ndownloader.figshare.com/files/2180111", "https://ndownloader.figshare.com/files/2180112", "https://ndownloader.figshare.com/files/2180113", "https://ndownloader.figshare.com/files/2180114", "https://ndownloader.figshare.com/files/2180115"], "description"=>"<div><p>Recently, extracts of <i>Dalbergia trichocarpa </i>bark have been shown to disrupt <i>P</i>. <i>aeruginosa </i>PAO1 quorum sensing (QS) mechanisms, which are key regulators of virulence factor expression and implicated in biofilm formation. One of the active compounds has been isolated and identified as oleanolic aldehyde coumarate (OALC), a novel bioactive compound that inhibits the formation of <i>P</i>. <i>aeruginosa</i> PAO1 biofilm and its maintenance as well as the expression of the <i>las</i> and <i>rhl</i> QS systems. Consequently, the production of QS-controlled virulence factors including, rhamnolipids, pyocyanin, elastase and extracellular polysaccharides as well as twitching and swarming motilities is reduced. Native acylhomoserine lactones (AHLs) production is inhibited by OALC but exogenous supply of AHLs does not restore the production of virulence factors by OALC-treated cultures, indicating that OALC exerts its effect beyond AHLs synthesis in the QS pathways. Further experiments provided a significant inhibition of the global virulence factor activator <i>gacA</i> by OALC. OALC disorganizes established biofilm structure and improves the bactericidal activity of tobramycin against biofilm-encapsulated PAO1 cells. Finally, a significant reduction of <i>Caenorhabditis elegans</i> paralysis was recorded when the worms were infected with OALC-pre-treated <i>P</i>. <i>aeruginosa</i>. Taken together, these results show that triterpenoid coumarate esters are suitable chemical backbones to target <i>P</i>. <i>aeruginosa</i> virulence mechanisms.</p></div>", "links"=>[], "tags"=>["results show", "triterpenoid coumarate esters", "Native acylhomoserine lactones", "extracellular polysaccharides", "rhl QS systems", "bactericidal activity", "virulence factor activator gacA", "Virulence factors", "biofilm formation", "virulence factor expression", "target P", "aeruginosa PAO 1 biofilm", "OALC disorganizes", "Tropical Legume", "Caenorhabditis elegans paralysis", "Dalbergia trichocarpa bark", "QS pathways", "exogenous supply", "chemical backbones", "Pseudomonas aeruginosa Biofilm Formation", "Triterpenoid Coumarate Ester", "aeruginosa virulence mechanisms", "oleanolic aldehyde coumarate", "biofilm structure", "aeruginosa PAO 1 quorum", "AHLs synthesis", "Dalbergia trichocarpa", "novel bioactive compound"], "article_id"=>1486583, "categories"=>["Biological Sciences", "Ecology"], "users"=>["Tsiry Rasamiravaka", "Olivier M. Vandeputte", "Laurent Pottier", "Joëlle Huet", "Christian Rabemanantsoa", "Martin Kiendrebeogo", "Abel Andriantsimahavandy", "Andry Rasamindrakotroka", "Caroline Stévigny", "Pierre Duez", "Mondher El Jaziri"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0132791.s001", "https://dx.doi.org/10.1371/journal.pone.0132791.s002", "https://dx.doi.org/10.1371/journal.pone.0132791.s003", "https://dx.doi.org/10.1371/journal.pone.0132791.s004", "https://dx.doi.org/10.1371/journal.pone.0132791.s005", "https://dx.doi.org/10.1371/journal.pone.0132791.s006", "https://dx.doi.org/10.1371/journal.pone.0132791.s007", "https://dx.doi.org/10.1371/journal.pone.0132791.s008", "https://dx.doi.org/10.1371/journal.pone.0132791.s009", "https://dx.doi.org/10.1371/journal.pone.0132791.s010", "https://dx.doi.org/10.1371/journal.pone.0132791.s011", "https://dx.doi.org/10.1371/journal.pone.0132791.s012"], "stats"=>{"downloads"=>47, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Pseudomonas_aeruginosa_Biofilm_Formation_and_Persistence_along_with_the_Production_of_Quorum_Sensing_Dependent_Virulence_Factors_Are_Disrupted_by_a_Triterpenoid_Coumarate_Ester_Isolated_from_Dalbergia_trichocarpa_a_Tropical_Legume/1486583", "title"=>"<i>Pseudomonas aeruginosa</i> Biofilm Formation and Persistence, along with the Production of Quorum Sensing-Dependent Virulence Factors, Are Disrupted by a Triterpenoid Coumarate Ester Isolated from <i>Dalbergia trichocarpa</i>, a Tropical Legume", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-07-17 02:51:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/2180065"], "description"=>"<p>(A) rhamnolipids production, (B) pyocyanin production, (C) Elastase production. The cell density of the bacteria was assessed at 600 nm and elastase production was assessed via an elastolysis assay and calculated as the ratio between <i>A</i><sub>495</sub> and <i>A</i><sub>600</sub>. The rhamnolipids production was measured using methylene-blue-based method and expressed in μg mL<sup>-1</sup>. Pyocyanin was extracted, quantified by absorbance measurements at 380 nm and calculated as the ratio between <i>A</i><sub>380</sub> and <i>A</i><sub>600</sub>. (D) Quantification of <i>N</i>-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12-HSL; grey bar) and <i>N</i>-butanoyl-L-homoserine lactone (C4-HSL; clear bar) produced by PAO1 cells. Acylhomoserine lactones were extracted and quantified by mass spectrometry. Error bars represent the standard errors of the means and all experiments were performed in quintuplicate with three independent assays and asterisks indicate samples that are significantly different from the DMSO controls (Student’s <i>t</i>-tests; <i>P</i> ≤ 0.01). Naringenin (Nar) and naringin (Nin) were used as a quorum sensing inhibitor control and negative control, respectively.</p>", "links"=>[], "tags"=>["results show", "triterpenoid coumarate esters", "Native acylhomoserine lactones", "extracellular polysaccharides", "rhl QS systems", "bactericidal activity", "virulence factor activator gacA", "Virulence factors", "biofilm formation", "virulence factor expression", "target P", "aeruginosa PAO 1 biofilm", "OALC disorganizes", "Tropical Legume", "Caenorhabditis elegans paralysis", "Dalbergia trichocarpa bark", "QS pathways", "exogenous supply", "chemical backbones", "Pseudomonas aeruginosa Biofilm Formation", "Triterpenoid Coumarate Ester", "aeruginosa virulence mechanisms", "oleanolic aldehyde coumarate", "biofilm structure", "aeruginosa PAO 1 quorum", "AHLs synthesis", "Dalbergia trichocarpa", "novel bioactive compound"], "article_id"=>1486546, "categories"=>["Biological Sciences", "Ecology"], "users"=>["Tsiry Rasamiravaka", "Olivier M. Vandeputte", "Laurent Pottier", "Joëlle Huet", "Christian Rabemanantsoa", "Martin Kiendrebeogo", "Abel Andriantsimahavandy", "Andry Rasamindrakotroka", "Caroline Stévigny", "Pierre Duez", "Mondher El Jaziri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0132791.g002", "stats"=>{"downloads"=>2, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_OALC_on_virulence_factors_and_acylhomoserine_lactones_production_in_P_aeruginosa_PAO1_/1486546", "title"=>"Effect of OALC on virulence factors and acylhomoserine lactones production in <i>P</i>. <i>aeruginosa</i> PAO1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-17 02:51:44"}

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Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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