HRP2 and pLDH-Based Rapid Diagnostic Tests, Expert Microscopy, and PCR for Detection of Malaria Infection during Pregnancy and at Delivery in Areas of Varied Transmission: A Prospective Cohort Study in Burkina Faso and Uganda
Publication Date
July 05, 2016
Journal
PLOS ONE
Authors
Daniel J. Kyabayinze, Issaka Zongo, Jane Cunningham, Michelle Gatton, et al
Volume
11
Issue
7
Pages
e0156954
DOI
http://doi.org/10.1371/journal.pone.0156954
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0156954
Web of Science
000379798700004
Scopus
84978044743
Mendeley
http://www.mendeley.com/research/hrp2-pldhbased-rapid-diagnostic-tests-expert-microscopy-pcr-detection-malaria-infection-during-pregn
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Mendeley | Further Information

{"title"=>"HRP2 and pLDH-based rapid diagnostic tests, expert microscopy, and PCR for detection of malaria infection during pregnancy and at delivery in areas of varied transmission: A prospective cohort study in Burkina Faso and Uganda", "type"=>"journal", "authors"=>[{"first_name"=>"Daniel J.", "last_name"=>"Kyabayinze", "scopus_author_id"=>"6506554844"}, {"first_name"=>"Issaka", "last_name"=>"Zongo", "scopus_author_id"=>"15842711900"}, {"first_name"=>"Jane", "last_name"=>"Cunningham", "scopus_author_id"=>"14119491900"}, {"first_name"=>"Michelle", "last_name"=>"Gatton", "scopus_author_id"=>"6701868710"}, {"first_name"=>"Patrick", "last_name"=>"Angutoko", "scopus_author_id"=>"55808783900"}, {"first_name"=>"John", "last_name"=>"Ategeka", "scopus_author_id"=>"55808541300"}, {"first_name"=>"Yves Daniel", "last_name"=>"Compaoré", "scopus_author_id"=>"55342017100"}, {"first_name"=>"Atis", "last_name"=>"Muehlenbachs", "scopus_author_id"=>"6506081106"}, {"first_name"=>"Jerry", "last_name"=>"Mulondo", "scopus_author_id"=>"55855921700"}, {"first_name"=>"Miriam", "last_name"=>"Nakalembe", "scopus_author_id"=>"35812415500"}, {"first_name"=>"Fabrice A.", "last_name"=>"Somé", "scopus_author_id"=>"56613519300"}, {"first_name"=>"Aminata", "last_name"=>"Ouattara", "scopus_author_id"=>"57190172717"}, {"first_name"=>"Noél", "last_name"=>"Rouamba", "scopus_author_id"=>"9432671600"}, {"first_name"=>"Jean Bosco", "last_name"=>"Ouédraogo", "scopus_author_id"=>"7003494281"}, {"first_name"=>"Heidi", "last_name"=>"Hopkins", "scopus_author_id"=>"35860066400"}, {"first_name"=>"David", "last_name"=>"Bell", "scopus_author_id"=>"8727316100"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"611146721", "scopus"=>"2-s2.0-84978044743", "issn"=>"19326203", "pmid"=>"27380525", "isbn"=>"1932-6203", "sgr"=>"84978044743", "doi"=>"10.1371/journal.pone.0156954"}, "id"=>"20dc18c8-e51a-3cdb-bc9c-125b9d637bcd", "abstract"=>"BACKGROUND: Intermittent screening and treatment (IST) of malaria during pregnancy has been proposed as an alternative to intermittent preventive treatment in pregnancy (IPTp), where IPTp is failing due to drug resistance. However, the antenatal parasitaemias are frequently very low, and the most appropriate screening test for IST has not been defined. METHODOLOGY/PRINCIPAL FINDINGS: We conducted a multi-center prospective study of 990 HIV-uninfected women attending ANC in two different malaria transmission settings at Tororo District Hospital, eastern Uganda and Colsama Health Center in western Burkina Faso. Women were enrolled in the study in the second or third trimester of pregnancy and followed to delivery, generating 2,597 blood samples for analysis. Screening tests included rapid diagnostic tests (RDTs) targeting histidine-rich protein 2 (HRP2) and parasite lactate dehydrogenase (pLDH) and microscopy, compared to nPCR as a reference standard. At enrolment, the proportion of pregnant women who were positive for P. falciparum by HRP2/pan pLDH RDT, Pf pLDH/pan pLDH RDT, microscopy and PCR was 38%, 29%, 36% and 44% in Uganda and 21%, 16%, 15% and 35% in Burkina Faso, respectively. All test positivity rates declined during follow-up. In comparison to PCR, the sensitivity of the HRP2/pan pLDH RDT, Pf pLDH/pan pLDH RDT and microscopy was 75.7%, 60.1% and 69.7% in Uganda, 55.8%, 42.6% and 55.8% in Burkina Faso respectively for all antenatal visits. Specificity was greater than 96% for all three tests. Comparison of accuracy using generalized estimating equation revealed that the HRP2- detecting RDT was the most accurate test in both settings. CONCLUSIONS/SIGNIFICANCE: The study suggests that HRP2-based RDTs are the most appropriate point-of-care test currently available for use during pregnancy especially for symptomatic women, but will still miss some PCR-positive women. The clinical significance of these very low density infections needs to be better defined.", "link"=>"http://www.mendeley.com/research/hrp2-pldhbased-rapid-diagnostic-tests-expert-microscopy-pcr-detection-malaria-infection-during-pregn", "reader_count"=>34, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Librarian"=>1, "Researcher"=>7, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>5, "Student > Master"=>13, "Other"=>1, "Student > Bachelor"=>2, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Librarian"=>1, "Researcher"=>7, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>5, "Student > Master"=>13, "Other"=>1, "Student > Bachelor"=>2, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>4, "Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>4, "Nursing and Health Professions"=>2, "Agricultural and Biological Sciences"=>6, "Medicine and Dentistry"=>10, "Business, Management and Accounting"=>1, "Social Sciences"=>3, "Computer Science"=>1, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>10}, "Social Sciences"=>{"Social Sciences"=>3}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>6}, "Computer Science"=>{"Computer Science"=>1}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>2}, "Business, Management and Accounting"=>{"Business, Management and Accounting"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}, "Unspecified"=>{"Unspecified"=>4}}, "reader_count_by_country"=>{"Belgium"=>1}, "group_count"=>1}

Scopus | Further Information

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