Correction: Human alpha defensin 5 is a candidate biomarker to delineate inflammatory bowel disease
Publication Date
December 06, 2017
Journal
PLOS ONE
Authors
Amanda D. Williams, Olga Y. Korolkova, Amos M. Sakwe, Timothy M. Geiger, et al
Volume
12
Issue
12
Pages
e0189551
DOI
http://doi.org/10.1371/journal.pone.0189551
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0189551
Scopus
85037167821
Mendeley
http://www.mendeley.com/research/correction-human-alpha-defensin-5-candidate-biomarker-delineate-inflammatory-bowel-disease-plos-one
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Mendeley | Further Information

{"title"=>"Correction: Human alpha defensin 5 is a candidate biomarker to delineate inflammatory bowel disease (PLoS ONE (2017) 12:8 (e0179710) DOI: 10.1371/journal.pone.0179710)", "type"=>"generic", "authors"=>[{"first_name"=>"Amanda D.", "last_name"=>"Williams", "scopus_author_id"=>"14008988000"}, {"first_name"=>"Olga Y.", "last_name"=>"Korolkova", "scopus_author_id"=>"56575013000"}, {"first_name"=>"Amos M.", "last_name"=>"Sakwe", "scopus_author_id"=>"6603131076"}, {"first_name"=>"Timothy M.", "last_name"=>"Geiger", "scopus_author_id"=>"12794225300"}, {"first_name"=>"Samuel D.", "last_name"=>"James", "scopus_author_id"=>"55702966800"}, {"first_name"=>"Roberta L.", "last_name"=>"Muldoon", "scopus_author_id"=>"8578345000"}, {"first_name"=>"Alan J.", "last_name"=>"Herline", "scopus_author_id"=>"6603613676"}, {"first_name"=>"J. Shawn", "last_name"=>"Goodwin", "scopus_author_id"=>"56463492800"}, {"first_name"=>"Michael G.", "last_name"=>"Izban", "scopus_author_id"=>"6603044553"}, {"first_name"=>"Mary K.", "last_name"=>"Washington", "scopus_author_id"=>"7007171283"}, {"first_name"=>"Duane T.", "last_name"=>"Smoot", "scopus_author_id"=>"7003713350"}, {"first_name"=>"Billy R.", "last_name"=>"Ballard", "scopus_author_id"=>"7004514192"}, {"first_name"=>"Maria", "last_name"=>"Gazouli", "scopus_author_id"=>"26643294000"}, {"first_name"=>"Amosy E.", "last_name"=>"M’Koma", "scopus_author_id"=>"57195381592"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-85037167821", "pmid"=>"28817680", "doi"=>"10.1371/journal.pone.0189551", "pui"=>"619533647", "isbn"=>"1111111111", "sgr"=>"85037167821"}, "id"=>"6037d852-97f5-33c3-ac8c-3f43edf275ff", "abstract"=>"Inability to distinguish Crohn's colitis from ulcerative colitis leads to the diagnosis of indeterminate colitis. This greatly effects medical and surgical care of the patient because treatments for the two diseases vary. Approximately 30 percent of inflammatory bowel disease patients cannot be accurately diagnosed, increasing their risk of inappropriate treatment. We sought to determine whether transcriptomic patterns could be used to develop diagnostic biomarker(s) to delineate inflammatory bowel disease more accurately. Four patients groups were assessed via whole-transcriptome microarray, qPCR, Western blot, and immunohistochemistry for differential expression of Human alpha-Defensin-5. In addition, immunohistochemistry for Paneth cells and Lysozyme, a Paneth cell marker, was also performed. Aberrant expression of Human alpha-Defensin-5 levels using transcript, Western blot, and immunohistochemistry staining levels was significantly upregulated in Crohn's colitis, p< 0.0001. Among patients with indeterminate colitis, Human alpha-Defensin-5 is a reliable differentiator with a positive predictive value of 96 percent. We also observed abundant ectopic crypt Paneth cells in all colectomy tissue samples of Crohn's colitis patients. In a retrospective study, we show that Human alpha-Defensin-5 could be used in indeterminate colitis patients to determine if they have either ulcerative colitis (low levels of Human alpha-Defensin-5) or Crohn's colitis (high levels of Human alpha-Defensin-5). Twenty of 67 patients (30 percent) who underwent restorative proctocolectomy for definitive ulcerative colitis were clinically changed to de novo Crohn's disease. These patients were profiled by Human alpha-Defensin-5 immunohistochemistry. All patients tested strongly positive. In addition, we observed by both hematoxylin and eosin and Lysozyme staining, a large number of ectopic Paneth cells in the colonic crypt of Crohn's colitis patient samples. Our experiments are the first to show that Human alpha-Defensin-5 is a potential candidate biomarker to molecularly differentiate Crohn's colitis from ulcerative colitis, to our knowledge. These data give us both a potential diagnostic marker in Human alpha-Defensin-5 and insight to develop future mechanistic studies to better understand crypt biology in Crohn's colitis.", "link"=>"http://www.mendeley.com/research/correction-human-alpha-defensin-5-candidate-biomarker-delineate-inflammatory-bowel-disease-plos-one", "reader_count"=>8, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>2, "Student > Ph. D. Student"=>3, "Student > Master"=>1, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>2, "Student > Ph. D. Student"=>3, "Student > Master"=>1, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>3, "Medicine and Dentistry"=>2, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}}, "group_count"=>0}

Scopus | Further Information

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