Varicellovirus UL49.5 Proteins Differentially Affect the Function of the Transporter Associated with Antigen Processing, TAP
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Varicellovirus UL49.5 proteins differentially affect the function of the transporter associated with antigen processing, TAP", "type"=>"journal", "authors"=>[{"first_name"=>"Danijela", "last_name"=>"Koppers-Lalic", "scopus_author_id"=>"8617796700"}, {"first_name"=>"Marieke C.", "last_name"=>"Verweij", "scopus_author_id"=>"12244130500"}, {"first_name"=>"Andrea D.", "last_name"=>"Lipińska", "scopus_author_id"=>"8359725500"}, {"first_name"=>"Ying", "last_name"=>"Wang", "scopus_author_id"=>"24345565700"}, {"first_name"=>"Edwin", "last_name"=>"Quinten", "scopus_author_id"=>"6506977702"}, {"first_name"=>"Eric A.", "last_name"=>"Reits", "scopus_author_id"=>"6603655132"}, {"first_name"=>"Joachim", "last_name"=>"Koch", "scopus_author_id"=>"24721951100"}, {"first_name"=>"Sandra", "last_name"=>"Loch", "scopus_author_id"=>"24343913400"}, {"first_name"=>"Marisa Marcondes", "last_name"=>"Rezende", "scopus_author_id"=>"8359725800"}, {"first_name"=>"Franz", "last_name"=>"Daus", "scopus_author_id"=>"6602244995"}, {"first_name"=>"Krystyna", "last_name"=>"Bieńkowska-Szewczyk", "scopus_author_id"=>"6602489973"}, {"first_name"=>"Nikolaus", "last_name"=>"Osterrieder", "scopus_author_id"=>"7003644999"}, {"first_name"=>"Thomas C.", "last_name"=>"Mettenleiter", "scopus_author_id"=>"7006059985"}, {"first_name"=>"Mirjam H M", "last_name"=>"Heemskerk", "scopus_author_id"=>"7004561258"}, {"first_name"=>"Robert", "last_name"=>"Tampé", "scopus_author_id"=>"7004546498"}, {"first_name"=>"Jacques J.", "last_name"=>"Neefjes", "scopus_author_id"=>"16224387800"}, {"first_name"=>"Shafiqul I.", "last_name"=>"Chowdhury", "scopus_author_id"=>"55999078800"}, {"first_name"=>"Maaike E.", "last_name"=>"Ressing", "scopus_author_id"=>"6602087001"}, {"first_name"=>"Frans A M", "last_name"=>"Rijsewijk", "scopus_author_id"=>"7004111556"}, {"first_name"=>"Emmanuel J H J", "last_name"=>"Wiertz", "scopus_author_id"=>"7004370048"}], "year"=>2008, "source"=>"PLoS Pathogens", "identifiers"=>{"isbn"=>"1553-7374 (Electronic)\\r1553-7366 (Linking)", "doi"=>"10.1371/journal.ppat.1000080", "pui"=>"351811516", "sgr"=>"44949148014", "issn"=>"15537366", "pmid"=>"18516302", "scopus"=>"2-s2.0-44949148014"}, "id"=>"a1d26574-1dcf-3d1c-b585-cbea59dfec25", "abstract"=>"Cytotoxic T-lymphocytes play an important role in the protection against viral infections, which they detect through the recognition of virus-derived peptides, presented in the context of MHC class I molecules at the surface of the infected cell. The transporter associated with antigen processing (TAP) plays an essential role in MHC class I–restricted antigen presentation, as TAP imports peptides into the ER, where peptide loading of MHC class I molecules takes place. In this study, the UL49.5 proteins of the varicelloviruses bovine herpesvirus 1 (BHV-1), pseudorabies virus (PRV), and equine herpesvirus 1 and 4 (EHV-1 and EHV-4) are characterized as members of a novel class of viral immune evasion proteins. These UL49.5 proteins interfere with MHC class I antigen presentation by blocking the supply of antigenic peptides through inhibition of TAP. BHV-1, PRV, and EHV-1 recombinant viruses lacking UL49.5 no longer interfere with peptide transport. Combined with the observation that the individually expressed UL49.5 proteins block TAP as well, these data indicate that UL49.5 is the viral factor that is both necessary and sufficient to abolish TAP function during productive infection by these viruses. The mechanisms through which the UL49.5 proteins of BHV-1, PRV, EHV-1, and EHV-4 block TAP exhibit surprising diversity. BHV-1 UL49.5 targets TAP for proteasomal degradation, whereas EHV-1 and EHV-4 UL49.5 interfere with the binding of ATP to TAP. In contrast, TAP stability and ATP recruitment are not affected by PRV UL49.5, although it has the capacity to arrest the peptide transporter in a translocation-incompetent state, a property shared with the BHV-1 and EHV-1 UL49.5. Taken together, these results classify the UL49.5 gene products of BHV-1, PRV, EHV-1, and EHV-4 as members of a novel family of viral immune evasion proteins, inhibiting TAP through a variety of mechanisms.", "link"=>"http://www.mendeley.com/research/varicellovirus-ul495-proteins-differentially-affect-function-transporter-associated-antigen-processi", "reader_count"=>8, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Researcher"=>2, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>1, "Student > Master"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Researcher"=>2, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>1, "Student > Master"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>4, "Veterinary Science and Veterinary Medicine"=>1}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>2}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "reader_count_by_country"=>{"United States"=>1, "Brazil"=>1}, "group_count"=>1}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/44949148014"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/44949148014?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=44949148014&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=44949148014&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/44949148014", "dc:identifier"=>"SCOPUS_ID:44949148014", "eid"=>"2-s2.0-44949148014", "dc:title"=>"Varicellovirus UL49.5 proteins differentially affect the function of the transporter associated with antigen processing, TAP", "dc:creator"=>"Koppers-Lalic D.", "prism:publicationName"=>"PLoS Pathogens", "prism:issn"=>"15537366", "prism:eIssn"=>"15537374", "prism:volume"=>"4", "prism:issueIdentifier"=>"5", "prism:pageRange"=>nil, "prism:coverDate"=>"2008-05-01", "prism:coverDisplayDate"=>"May 2008", "prism:doi"=>"10.1371/journal.ppat.1000080", "citedby-count"=>"50", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Leiden University Medical Center - LUMC", "affiliation-city"=>"Leiden", "affiliation-country"=>"Netherlands"}, {"@_fa"=>"true", "affilname"=>"Leiden University Medical Center - LUMC", "affiliation-city"=>"Leiden", "affiliation-country"=>"Netherlands"}], "pubmed-id"=>"18516302", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e1000080", "source-id"=>"4000151809"}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplospathogens%2Farticle%3Fid%3D10.1371%252Fjournal.ppat.1000080", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"5", "year"=>"2008", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"6", "year"=>"2008", "pdf_views"=>"76", "xml_views"=>"1", "html_views"=>"280"}
  • {"month"=>"7", "year"=>"2008", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"8", "year"=>"2008", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"9", "year"=>"2008", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"66"}
  • {"month"=>"10", "year"=>"2008", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"11", "year"=>"2008", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"12", "year"=>"2008", "pdf_views"=>"16", "xml_views"=>"3", "html_views"=>"38"}
  • {"month"=>"1", "year"=>"2009", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"2", "year"=>"2009", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"3", "year"=>"2009", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"4", "year"=>"2009", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"5", "year"=>"2009", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"6", "year"=>"2009", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"7", "year"=>"2009", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"8", "year"=>"2009", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"9", "year"=>"2009", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"10", "year"=>"2009", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"11", "year"=>"2009", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"12", "year"=>"2009", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"1", "year"=>"2010", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"2", "year"=>"2010", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"3", "year"=>"2010", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"4", "year"=>"2010", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"17"}
  • {"month"=>"5", "year"=>"2010", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"6", "year"=>"2010", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2010", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"8", "year"=>"2010", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"9", "year"=>"2010", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"10", "year"=>"2010", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"11", "year"=>"2010", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"12", "year"=>"2010", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"1", "year"=>"2011", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"17"}
  • {"month"=>"2", "year"=>"2011", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"3", "year"=>"2011", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"4", "year"=>"2011", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"5", "year"=>"2011", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"6", "year"=>"2011", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"7", "year"=>"2011", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"8", "year"=>"2011", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"9", "year"=>"2011", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"10", "year"=>"2011", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"11", "year"=>"2011", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"37"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"29"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"29"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"36"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"3", "xml_views"=>"5", "html_views"=>"16"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"83"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"214"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"68"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"28"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"57"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"33"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"3", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"29"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/930077"], "description"=>"<p>MDBK cells were mock-infected or infected with wild type BHV-1 or BHV-1 expressing UL49.5Δtail for 12 hours. The levels of bovine TAP1 and, as a control, α-tubulin were assessed in lysates of infected cells by immunoblotting (IB). BHV-1 UL49.5 was immunoprecipitated from lysates of metabolically labeled cells (IP).</p>", "links"=>[], "tags"=>["bovine", "mediated", "cytoplasmic"], "article_id"=>600510, "categories"=>["Immunology", "Infectious Diseases"], "users"=>["Danijela Koppers-Lalic", "Marieke C. Verweij", "Andrea D. Lipińska", "Ying Wang", "Edwin Quinten", "Eric A. Reits", "Joachim Koch", "Sandra Loch", "Marisa Marcondes Rezende", "Franz Daus", "Krystyna Bieńkowska-Szewczyk", "Nikolaus Osterrieder", "Thomas C. Mettenleiter", "Mirjam H. M. Heemskerk", "Robert Tampé", "Jacques J. Neefjes", "Shafiqul I. Chowdhury", "Maaike E. Ressing", "Frans A. M. Rijsewijk", "Emmanuel J. H. J. Wiertz"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000080.g005", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Degradation_of_bovine_TAP_is_mediated_by_BHV_1UL49_5_through_its_cytoplasmic_tail_/600510", "title"=>"Degradation of bovine TAP is mediated by BHV-1UL49.5 through its cytoplasmic tail.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 10:19:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/929981"], "description"=>"<p>Bovine cells (MDBK), porcine cells (PK15), and equine cells (E.derm) were infected with wild type BHV-1, PRV, or EHV-1, respectively, or with the corresponding UL49.5-negative recombinant viruses. In all experiments, mock-treated (uninfected) cells from the relevant host species were used as a control. Peptide transport was assessed at 5 hrs post-infection in the presence and absence of ATP (black and open bars, respectively). The data are expressed as percentage of translocation, relative to the translocation observed in control cells (defined as 100%).</p>", "links"=>[], "tags"=>["tap-inhibition", "virus-infected"], "article_id"=>600411, "categories"=>["Immunology", "Infectious Diseases"], "users"=>["Danijela Koppers-Lalic", "Marieke C. Verweij", "Andrea D. Lipińska", "Ying Wang", "Edwin Quinten", "Eric A. Reits", "Joachim Koch", "Sandra Loch", "Marisa Marcondes Rezende", "Franz Daus", "Krystyna Bieńkowska-Szewczyk", "Nikolaus Osterrieder", "Thomas C. Mettenleiter", "Mirjam H. M. Heemskerk", "Robert Tampé", "Jacques J. Neefjes", "Shafiqul I. Chowdhury", "Maaike E. Ressing", "Frans A. M. Rijsewijk", "Emmanuel J. H. J. Wiertz"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000080.g004", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_UL49_5_is_responsible_for_TAP_inhibition_in_virus_infected_natural_host_cells_/600411", "title"=>"UL49.5 is responsible for TAP-inhibition in virus-infected natural host cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 10:19:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/930555"], "description"=>"<p>The lateral mobility of the TAP complex was analyzed in MJS TAP1-GFP cells using confocal microscopy and FRAP. A circular spot in the ER was bleached and recovery of fluorescence was monitored. The half-time for recovery was determined and used to calculate the diffusion coefficient D. Where indicated, ATP was depleted (-ATP) or saturating amounts of substrate peptides (long side chain peptides, l.s.c.p.) were micro-injected into the cells.</p>", "links"=>[], "tags"=>["homologs", "translocation-incompetent"], "article_id"=>600980, "categories"=>["Immunology", "Infectious Diseases"], "users"=>["Danijela Koppers-Lalic", "Marieke C. Verweij", "Andrea D. Lipińska", "Ying Wang", "Edwin Quinten", "Eric A. Reits", "Joachim Koch", "Sandra Loch", "Marisa Marcondes Rezende", "Franz Daus", "Krystyna Bieńkowska-Szewczyk", "Nikolaus Osterrieder", "Thomas C. Mettenleiter", "Mirjam H. M. Heemskerk", "Robert Tampé", "Jacques J. Neefjes", "Shafiqul I. Chowdhury", "Maaike E. Ressing", "Frans A. M. Rijsewijk", "Emmanuel J. H. J. Wiertz"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000080.g009", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_UL49_5_homologs_arrest_TAP_in_a_translocation_incompetent_state_/600980", "title"=>"UL49.5 homologs arrest TAP in a translocation-incompetent state.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 10:22:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/930457"], "description"=>"<p>(A, B, C) MJS cells expressing the UL49.5 proteins or the HCMV-derived TAP-inhibitor US6 and (D) E.derm cells expressing EHV-4 UL49.5 were lysed using digitonin or NP40 as indicated. Post nuclear lysates were incubated with ATP-agarose. The pellet (P) contains the ATP-binding proteins. The supernatant (S) contains proteins incapable of binding ATP. ATP-bound (ATP-agarose beads; pellet) and unbound (soluble; supernatant) fractions were separated by centrifugation and analyzed using SDS-PAGE and immunoblotting (IB) with antibodies against the proteins indicated.</p>", "links"=>[], "tags"=>["ehv-1", "ehv-4", "atp", "binding", "equine"], "article_id"=>600879, "categories"=>["Immunology", "Infectious Diseases"], "users"=>["Danijela Koppers-Lalic", "Marieke C. Verweij", "Andrea D. Lipińska", "Ying Wang", "Edwin Quinten", "Eric A. Reits", "Joachim Koch", "Sandra Loch", "Marisa Marcondes Rezende", "Franz Daus", "Krystyna Bieńkowska-Szewczyk", "Nikolaus Osterrieder", "Thomas C. Mettenleiter", "Mirjam H. M. Heemskerk", "Robert Tampé", "Jacques J. Neefjes", "Shafiqul I. Chowdhury", "Maaike E. Ressing", "Frans A. M. Rijsewijk", "Emmanuel J. H. J. Wiertz"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000080.g008", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_UL49_5_of_EHV_1_and_EHV_4_block_ATP_binding_to_human_and_equine_TAP_/600879", "title"=>"UL49.5 of EHV-1 and EHV-4 block ATP binding to human and equine TAP.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 10:21:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/929747"], "description"=>"<p>(A) MHC class I expression in cells from the relevant host species stably expressing the UL49.5 homologs of BHV-1, PRV, EHV-1, EHV-4, CHV and VZV. Cells of bovine (MDBK), porcine (PK15), equine (E.derm), canine (MDCK), and human origin (MJS) were transduced with retroviruses expressing the respective UL49.5 proteins. Surface MHC class I molecules were stained with specific antibodies and analyzed using flow cytometry. Shown are the MHC class I levels on cells without UL49.5 (thin line) and with UL49.5 (boldface line); dashed line, goat anti-mouse phycoerythrin control. (B) TAP-dependent peptide transport is inhibited in cells from the relevant host species expressing BHV-1, PRV, EHV-1, EHV-4 and CHV UL49.5, but not in MJS cells expressing VZV UL49.5. Peptide transport was assessed in the presence and absence of ATP (black and open bars, respectively). Peptide transport is expressed as percentage of translocation, relative to the translocation observed in control cells (defined as 100%). The standard deviations are represented by the error bars. A difference at p<0.05 was considered significant.</p>", "links"=>[], "tags"=>["inhibition", "homologs"], "article_id"=>600176, "categories"=>["Immunology", "Infectious Diseases"], "users"=>["Danijela Koppers-Lalic", "Marieke C. Verweij", "Andrea D. Lipińska", "Ying Wang", "Edwin Quinten", "Eric A. Reits", "Joachim Koch", "Sandra Loch", "Marisa Marcondes Rezende", "Franz Daus", "Krystyna Bieńkowska-Szewczyk", "Nikolaus Osterrieder", "Thomas C. Mettenleiter", "Mirjam H. M. Heemskerk", "Robert Tampé", "Jacques J. Neefjes", "Shafiqul I. Chowdhury", "Maaike E. Ressing", "Frans A. M. Rijsewijk", "Emmanuel J. H. J. Wiertz"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000080.g002", "stats"=>{"downloads"=>1, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_TAP_inhibition_by_Varicellovirus_encoded_UL49_5_homologs_in_natural_host_cells_/600176", "title"=>"TAP inhibition by <i>Varicellovirus</i>-encoded UL49.5 homologs in natural host cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 10:17:33"}
  • {"files"=>["https://ndownloader.figshare.com/files/930665"], "description"=>"<p>The ClustalV (PAM250) method was used under the default settings of the alignment program MegAlignTM 500 of the sequence analysis software DNA* of DNASTAR Inc. The UL49.5 proteins for which TAP inhibition has been tested are shown in bold. *The HHV-3 (VZV) UL49.5 protein binds TAP, but does not block peptide transport. The NCBI accession numbers are provided in <a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1000080#s4\" target=\"_blank\">Materials and Methods</a>.</p>", "links"=>[], "tags"=>["alphaherpesvirus", "proteins", "constructed", "presently", "amino", "sequences"], "article_id"=>601085, "categories"=>["Immunology", "Infectious Diseases"], "users"=>["Danijela Koppers-Lalic", "Marieke C. Verweij", "Andrea D. Lipińska", "Ying Wang", "Edwin Quinten", "Eric A. Reits", "Joachim Koch", "Sandra Loch", "Marisa Marcondes Rezende", "Franz Daus", "Krystyna Bieńkowska-Szewczyk", "Nikolaus Osterrieder", "Thomas C. Mettenleiter", "Mirjam H. M. Heemskerk", "Robert Tampé", "Jacques J. Neefjes", "Shafiqul I. Chowdhury", "Maaike E. Ressing", "Frans A. M. Rijsewijk", "Emmanuel J. H. J. Wiertz"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000080.g010", "stats"=>{"downloads"=>1, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Phylogenetic_tree_of_alphaherpesvirus_UL49_5_proteins_constructed_on_the_basis_of_all_presently_known_UL49_5_amino_acid_sequences_of_Alphaherpesvirinae_using_ClustalV_/601085", "title"=>"Phylogenetic tree of alphaherpesvirus UL49.5 proteins constructed on the basis of all presently known UL49.5 amino acid sequences of <i>Alphaherpesvirinae</i> using ClustalV.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 10:23:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/930319"], "description"=>"<p>To evaluate peptide binding to the TAP complex, microsomal membranes from MJS TAP1-GFP cells (control; ▪), or MJS TAP1-GFP cells expressing UL49.5 of PRV (▴) or EHV-1 (▾) were incubated with increasing concentrations of the radiolabeled peptide (RR[<sup>125</sup>I]YQKSTEL). Unspecific binding was determined in the presence of 200-fold excess of ICP47 (data not shown). The amount of specifically bound peptide per amount of microsomal protein is plotted against the peptide concentration. K<sub>d</sub> values for control MJS: 277±58 nM, for MJS UL49.5<sup>PRV</sup>: 351±42 nM and for MJS UL49.5<sup>EHV-1</sup>: 236±71 nM.</p>", "links"=>[], "tags"=>["prv", "ehv-1", "peptide", "binding"], "article_id"=>600746, "categories"=>["Immunology", "Infectious Diseases"], "users"=>["Danijela Koppers-Lalic", "Marieke C. Verweij", "Andrea D. Lipińska", "Ying Wang", "Edwin Quinten", "Eric A. Reits", "Joachim Koch", "Sandra Loch", "Marisa Marcondes Rezende", "Franz Daus", "Krystyna Bieńkowska-Szewczyk", "Nikolaus Osterrieder", "Thomas C. Mettenleiter", "Mirjam H. M. Heemskerk", "Robert Tampé", "Jacques J. Neefjes", "Shafiqul I. Chowdhury", "Maaike E. Ressing", "Frans A. M. Rijsewijk", "Emmanuel J. H. J. Wiertz"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000080.g007", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_UL49_5_of_PRV_and_EHV_1_do_not_interfere_with_peptide_binding_to_TAP_/600746", "title"=>"UL49.5 of PRV and EHV-1 do not interfere with peptide binding to TAP.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 10:21:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/929654"], "description"=>"<p>Hydrophobic residues indicative of N-terminal signal sequences (dashed line) and membrane anchor regions (bold line) are indicated. The conserved cysteine residue involved in disulfide bond formation with the viral glycoprotein M is indicated. The amino acid sequence alignment of UL49.5 homologs was performed using Vector<i>NTI</i> software (Invitrogen).</p>", "links"=>[], "tags"=>["amino", "sequences", "homologs", "alphaherpesviruses", "accession", "numbers", "summarized"], "article_id"=>600055, "categories"=>["Immunology", "Infectious Diseases"], "users"=>["Danijela Koppers-Lalic", "Marieke C. Verweij", "Andrea D. Lipińska", "Ying Wang", "Edwin Quinten", "Eric A. Reits", "Joachim Koch", "Sandra Loch", "Marisa Marcondes Rezende", "Franz Daus", "Krystyna Bieńkowska-Szewczyk", "Nikolaus Osterrieder", "Thomas C. Mettenleiter", "Mirjam H. M. Heemskerk", "Robert Tampé", "Jacques J. Neefjes", "Shafiqul I. Chowdhury", "Maaike E. Ressing", "Frans A. M. Rijsewijk", "Emmanuel J. H. J. Wiertz"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000080.g001", "stats"=>{"downloads"=>1, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Alignment_of_the_amino_acid_sequences_of_UL49_5_homologs_of_a_selection_of_alphaherpesviruses_NCBI_accession_numbers_are_summarized_in_Materials_and_Methods_/600055", "title"=>"Alignment of the amino acid sequences of UL49.5 homologs of a selection of alphaherpesviruses (NCBI accession numbers are summarized in Materials and Methods).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 10:16:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/930232"], "description"=>"<p>(A) TAP-dependent peptide transport is inhibited in human melanoma cells (MJS) stably expressing the UL49.5 homologs of BHV-1, PRV and EHV-1, but not CHV. Peptide transport is depicted as percentage of translocation, relative to the translocation observed in control cells (defined as 100%). (B) Steady state protein levels of TAP1, TAP2, MHC class I heavy chains (MHC I HC), and UL49.5 in control cells and cell lines expressing UL49.5 of BHV-1, PRV and EHV-1. Proteins present in post-nuclear supernatants were separated using SDS-PAGE and detected by immunoblotting (IB) using antibodies specific for TAP1, TAP2, MHC class I heavy chains, and the UL49.5 proteins of BHV-1, PRV and EHV-1. *The doublet of PRV UL49.5 is probably related to differential glycosylation.</p>", "links"=>[], "tags"=>["bhv-1", "prv", "ehv-1", "tap1", "tap2"], "article_id"=>600664, "categories"=>["Immunology", "Infectious Diseases"], "users"=>["Danijela Koppers-Lalic", "Marieke C. Verweij", "Andrea D. Lipińska", "Ying Wang", "Edwin Quinten", "Eric A. Reits", "Joachim Koch", "Sandra Loch", "Marisa Marcondes Rezende", "Franz Daus", "Krystyna Bieńkowska-Szewczyk", "Nikolaus Osterrieder", "Thomas C. Mettenleiter", "Mirjam H. M. Heemskerk", "Robert Tampé", "Jacques J. Neefjes", "Shafiqul I. Chowdhury", "Maaike E. Ressing", "Frans A. M. Rijsewijk", "Emmanuel J. H. J. Wiertz"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000080.g006", "stats"=>{"downloads"=>0, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_UL49_5_of_BHV_1_but_not_of_PRV_or_EHV_1_reduces_TAP1_and_TAP2_steady_state_levels_in_human_cells_/600664", "title"=>"UL49.5 of BHV-1 but not of PRV or EHV-1 reduces TAP1 and TAP2 steady state levels in human cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 10:20:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/929845"], "description"=>"<p>(A) TAP1 was immunoprecipitated (IP) from MJS cells expressing EHV-1 or VZV UL49.5 proteins. Co-precipitating UL49.5 proteins were analyzed by immunoblotting (IB) using antibodies against EHV-1 or VZV UL49.5. Left panel: cell lysates were loaded on SDS-PAGE directly and stained by immunoblotting. (B) BHV-1 or VZV UL49.5-expressing PHA-treated T-cell blasts were labeled with <sup>51</sup>Cr and used as target cells for CTL clones specific for the minor histocompatibility antigens HY and HA-2, recognized in the context of HLA-A1 and HLA-A2, respectively. Specific lysis was determined by measuring <sup>51</sup>Cr release from the target cells after 4 hrs. Effector/target (E/T) ratios are indicated. (C) PHA-treated T-cell blasts or a melanoma cell line (Mel518) carrying the HY antigen were incubated with the HY-specific CTL clone HY HLA-A1. IFN-γ levels released by the CTL clones were determined from the supernatants of the co-cultures after 24 hrs.</p>", "links"=>[], "tags"=>["interacts", "peptide-loading", "inhibit", "mhc", "i-restricted"], "article_id"=>600271, "categories"=>["Immunology", "Infectious Diseases"], "users"=>["Danijela Koppers-Lalic", "Marieke C. Verweij", "Andrea D. Lipińska", "Ying Wang", "Edwin Quinten", "Eric A. Reits", "Joachim Koch", "Sandra Loch", "Marisa Marcondes Rezende", "Franz Daus", "Krystyna Bieńkowska-Szewczyk", "Nikolaus Osterrieder", "Thomas C. Mettenleiter", "Mirjam H. M. Heemskerk", "Robert Tampé", "Jacques J. Neefjes", "Shafiqul I. Chowdhury", "Maaike E. Ressing", "Frans A. M. Rijsewijk", "Emmanuel J. H. J. Wiertz"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000080.g003", "stats"=>{"downloads"=>1, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_VZV_UL49_5_interacts_with_the_peptide_loading_complex_but_does_not_inhibit_MHC_class_I_restricted_T_cell_recognition_/600271", "title"=>"VZV UL49.5 interacts with the peptide-loading complex, but does not inhibit MHC class I-restricted T cell recognition.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 10:18:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/930760"], "description"=>"<p>PCR primers used</p>", "links"=>[], "tags"=>["primers"], "article_id"=>601185, "categories"=>["Immunology", "Infectious Diseases"], "users"=>["Danijela Koppers-Lalic", "Marieke C. Verweij", "Andrea D. Lipińska", "Ying Wang", "Edwin Quinten", "Eric A. Reits", "Joachim Koch", "Sandra Loch", "Marisa Marcondes Rezende", "Franz Daus", "Krystyna Bieńkowska-Szewczyk", "Nikolaus Osterrieder", "Thomas C. Mettenleiter", "Mirjam H. M. Heemskerk", "Robert Tampé", "Jacques J. Neefjes", "Shafiqul I. Chowdhury", "Maaike E. Ressing", "Frans A. M. Rijsewijk", "Emmanuel J. H. J. Wiertz"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000080.t001", "stats"=>{"downloads"=>7, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_PCR_primers_used_/601185", "title"=>"PCR primers used", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-21 10:23:43"}

PMC Usage Stats | Further Information

  • {"month"=>"1", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"16", "year"=>"2010", "pdf"=>"5", "unique-ip"=>"13", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"2", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"14", "unique-ip"=>"15", "pdf"=>"7", "year"=>"2010", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"3", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"11", "year"=>"2010", "pdf"=>"7", "unique-ip"=>"12", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"4", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"12", "unique-ip"=>"11", "pdf"=>"8", "year"=>"2010", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"5", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"8", "year"=>"2010", "pdf"=>"7", "unique-ip"=>"9", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"6", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"8", "unique-ip"=>"9", "pdf"=>"5", "year"=>"2010", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"7", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"15", "year"=>"2010", "pdf"=>"7", "unique-ip"=>"12", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"8", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"9", "unique-ip"=>"10", "pdf"=>"10", "year"=>"2010", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"9", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"5", "full-text"=>"8", "year"=>"2010", "pdf"=>"8", "unique-ip"=>"16", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"10", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"7", "unique-ip"=>"11", "pdf"=>"4", "year"=>"2010", "figure"=>"10", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"11", "scanned-page-browse"=>"0", "cited-by"=>"1", "abstract"=>"2", "full-text"=>"12", "year"=>"2010", "pdf"=>"5", "unique-ip"=>"12", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"12", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"18", "unique-ip"=>"15", "pdf"=>"3", "year"=>"2010", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"1", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"21", "year"=>"2011", "pdf"=>"10", "unique-ip"=>"18", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"2", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"7", "unique-ip"=>"10", "pdf"=>"2", "year"=>"2011", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"3", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"4", "full-text"=>"10", "year"=>"2011", "pdf"=>"2", "unique-ip"=>"7", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"4", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"9", "unique-ip"=>"9", "pdf"=>"5", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"5", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"6", "full-text"=>"16", "year"=>"2011", "pdf"=>"6", "unique-ip"=>"16", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"6", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"6", "unique-ip"=>"9", "pdf"=>"3", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"7", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"5", "year"=>"2011", "pdf"=>"1", "unique-ip"=>"5", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"8", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"7", "unique-ip"=>"8", "pdf"=>"5", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"9", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"8", "year"=>"2011", "pdf"=>"6", "unique-ip"=>"8", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"10", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"4", "unique-ip"=>"4", "pdf"=>"1", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"11", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"6", "year"=>"2011", "pdf"=>"1", "unique-ip"=>"6", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"12", "cited-by"=>"1", "abstract"=>"0", "full-text"=>"13", "unique-ip"=>"14", "pdf"=>"4", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"1", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"16", "year"=>"2012", "pdf"=>"3", "unique-ip"=>"13", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"2", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"9", "unique-ip"=>"9", "pdf"=>"1", "year"=>"2012", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"3", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"9", "year"=>"2012", "pdf"=>"4", "unique-ip"=>"8", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"4", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"3", "unique-ip"=>"3", "pdf"=>"0", "year"=>"2012", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"5", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"7", "year"=>"2012", "pdf"=>"1", "unique-ip"=>"8", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"23", "month"=>"6", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"0", "unique-ip"=>"0", "pdf"=>"0", "year"=>"2008", "figure"=>"53", "scanned-summary"=>"30", "supp-data"=>"30"}
  • {"month"=>"7", "scanned-page-browse"=>"16", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"2", "year"=>"2008", "pdf"=>"0", "unique-ip"=>"7", "figure"=>"56", "scanned-summary"=>"31", "supp-data"=>"32"}
  • {"scanned-page-browse"=>"24", "month"=>"8", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"1", "unique-ip"=>"0", "pdf"=>"0", "year"=>"2008", "figure"=>"58", "scanned-summary"=>"33", "supp-data"=>"27"}
  • {"month"=>"9", "scanned-page-browse"=>"20", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"4", "year"=>"2008", "pdf"=>"0", "unique-ip"=>"18", "figure"=>"80", "scanned-summary"=>"38", "supp-data"=>"35"}
  • {"scanned-page-browse"=>"0", "month"=>"10", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"23", "unique-ip"=>"24", "pdf"=>"17", "year"=>"2008", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"11", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"27", "year"=>"2008", "pdf"=>"13", "unique-ip"=>"16", "figure"=>"13", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"12", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"21", "unique-ip"=>"21", "pdf"=>"11", "year"=>"2008", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"1", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"111", "year"=>"2009", "pdf"=>"43", "unique-ip"=>"76", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"2", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"20", "unique-ip"=>"19", "pdf"=>"10", "year"=>"2009", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"3", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"28", "year"=>"2009", "pdf"=>"18", "unique-ip"=>"27", "figure"=>"14", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"4", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"27", "unique-ip"=>"26", "pdf"=>"8", "year"=>"2009", "figure"=>"11", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"5", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"21", "year"=>"2009", "pdf"=>"6", "unique-ip"=>"19", "figure"=>"12", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"6", "cited-by"=>"0", "abstract"=>"5", "full-text"=>"18", "unique-ip"=>"22", "pdf"=>"10", "year"=>"2009", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"7", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"4", "full-text"=>"12", "year"=>"2009", "pdf"=>"5", "unique-ip"=>"13", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"8", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"11", "unique-ip"=>"11", "pdf"=>"3", "year"=>"2009", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"9", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"16", "year"=>"2009", "pdf"=>"9", "unique-ip"=>"14", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"10", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"8", "unique-ip"=>"8", "pdf"=>"5", "year"=>"2009", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"11", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"19", "year"=>"2009", "pdf"=>"12", "unique-ip"=>"22", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"12", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"24", "unique-ip"=>"22", "pdf"=>"13", "year"=>"2009", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"8", "year"=>"2012", "pdf"=>"5", "unique-ip"=>"10", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"24", "full-text"=>"30", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"19", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"14", "full-text"=>"18", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"15", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"17", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"15", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"12", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"16", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"17", "full-text"=>"21", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"17", "full-text"=>"28", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"15", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"16", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"14", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"18", "full-text"=>"18", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"21", "full-text"=>"23", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"13", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"14", "full-text"=>"17", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}

Relative Metric

{"start_date"=>"2008-01-01T00:00:00Z", "end_date"=>"2008-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[324, 674, 863, 1015, 1146, 1259, 1360, 1452, 1540, 1621, 1704, 1776, 1843, 1905, 1968, 2029, 2097, 2162, 2233, 2289, 2352, 2412, 2476, 2538, 2601, 2655, 2714, 2777, 2841, 2894, 2946, 2998, 3047, 3103, 3151, 3201, 3258, 3317, 3369, 3424, 3471, 3521, 3583, 3625, 3687, 3743, 3788, 3838, 3896, 3953, 4015, 4075, 4148, 4201, 4275, 4347, 4420, 4486, 4535, 4592, 4660, 4731, 4792, 4845, 4901, 4971, 5027, 5089, 5148, 5200, 5261, 5318, 5374]}, {"subject_area"=>"/Biology and life sciences/Immunology", "average_usage"=>[285, 598, 757, 913, 1055, 1163, 1259, 1351, 1443, 1527, 1591, 1650, 1720, 1787, 1856, 1918, 1966, 2017, 2087, 2137, 2201, 2254, 2314, 2370, 2437, 2490, 2549, 2602, 2665, 2701, 2754, 2801, 2844, 2894, 2939, 3003, 3050, 3112, 3157, 3196, 3242, 3284, 3335, 3388, 3440, 3490, 3533, 3577, 3630, 3678, 3724, 3773, 3827, 3881, 3931, 3983, 4041, 4096, 4150, 4223, 4269, 4299, 4346, 4400, 4478, 4525, 4602, 4652, 4721, 4783, 4833, 4872, 4908]}]}

F1000Prime | Further Information

Loading … Spinner
There are currently no alerts