NUP-1 Is a Large Coiled-Coil Nucleoskeletal Protein in Trypanosomes with Lamin-Like Functions
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{"title"=>"NUP-1 is a large coiled-coil nucleoskeletal protein in trypanosomes with lamin-like functions", "type"=>"journal", "authors"=>[{"first_name"=>"Kelly N.", "last_name"=>"DuBois", "scopus_author_id"=>"35310077800"}, {"first_name"=>"Sam", "last_name"=>"Alsford", "scopus_author_id"=>"6505963776"}, {"first_name"=>"Jennifer M.", "last_name"=>"Holden", "scopus_author_id"=>"37021351200"}, {"first_name"=>"Johanna", "last_name"=>"Buisson", "scopus_author_id"=>"14622324400"}, {"first_name"=>"Michal", "last_name"=>"Swiderski", "scopus_author_id"=>"56630880800"}, {"first_name"=>"Jean Mathieu", "last_name"=>"Bart", "scopus_author_id"=>"7006841817"}, {"first_name"=>"Alexander V.", "last_name"=>"Ratushny", "scopus_author_id"=>"6508327477"}, {"first_name"=>"Yakun", "last_name"=>"Wan", "scopus_author_id"=>"24328930500"}, {"first_name"=>"Philippe", "last_name"=>"Bastin", "scopus_author_id"=>"55917121900"}, {"first_name"=>"J. David", "last_name"=>"Barry", "scopus_author_id"=>"7402082853"}, {"first_name"=>"Miguel", "last_name"=>"Navarro", "scopus_author_id"=>"35234388700"}, {"first_name"=>"David", "last_name"=>"Horn", "scopus_author_id"=>"36145944800"}, {"first_name"=>"John D.", "last_name"=>"Aitchison", "scopus_author_id"=>"56384164700"}, {"first_name"=>"Michael P.", "last_name"=>"Rout", "scopus_author_id"=>"7004273135"}, {"first_name"=>"Mark C.", "last_name"=>"Field", "scopus_author_id"=>"7201475745"}], "year"=>2012, "source"=>"PLoS Biology", "identifiers"=>{"issn"=>"15449173", "scopus"=>"2-s2.0-84858966521", "pui"=>"364514747", "doi"=>"10.1371/journal.pbio.1001287", "isbn"=>"1545-7885 (Electronic)\\r1544-9173 (Linking)", "sgr"=>"84858966521", "pmid"=>"22479148"}, "id"=>"196f3ea0-b74e-3f2d-86f5-14b4f67122bf", "abstract"=>"... Figure 1. NUP - 1 is a large coiled coil protein identified in T . brucei and restricted to trypanosomatids. ... NUP - 1 mRNA is expressed at similar levels in BSF and PCF T . brucei , indicating a role throughout the life cycle (Figure S1). ... \\n", "link"=>"http://www.mendeley.com/research/nup1-large-coiledcoil-nucleoskeletal-protein-trypanosomes-laminlike-functions", "reader_count"=>91, "reader_count_by_academic_status"=>{"Unspecified"=>4, "Professor > Associate Professor"=>6, "Researcher"=>20, "Student > Doctoral Student"=>6, "Student > Ph. D. Student"=>26, "Student > Postgraduate"=>3, "Student > Master"=>10, "Other"=>2, "Student > Bachelor"=>5, "Lecturer"=>2, "Lecturer > Senior Lecturer"=>1, "Professor"=>6}, "reader_count_by_user_role"=>{"Unspecified"=>4, "Professor > Associate Professor"=>6, "Researcher"=>20, "Student > Doctoral Student"=>6, "Student > Ph. D. Student"=>26, "Student > Postgraduate"=>3, "Student > Master"=>10, "Other"=>2, "Student > Bachelor"=>5, "Lecturer"=>2, "Lecturer > Senior Lecturer"=>1, "Professor"=>6}, "reader_count_by_subject_area"=>{"Unspecified"=>6, "Biochemistry, Genetics and Molecular Biology"=>14, "Agricultural and Biological Sciences"=>63, "Medicine and Dentistry"=>3, "Chemistry"=>1, "Social Sciences"=>1, "Immunology and Microbiology"=>3}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Chemistry"=>{"Chemistry"=>1}, "Social Sciences"=>{"Social Sciences"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>63}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>14}, "Unspecified"=>{"Unspecified"=>6}}, "reader_count_by_country"=>{"Austria"=>1, "Japan"=>1, "Brazil"=>2, "United Kingdom"=>4, "France"=>1, "Switzerland"=>1}, "group_count"=>2}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/662750"], "description"=>"<p>(A) Control cell nucleus. (B–C) Nuclear morphology disruption in NUP-1 RNAi cells, with nuclei producing asymmetric extensions and/or invaginations. (D) NPC arrays (black arrow) are visible. (E) Areas of the nuclear envelope appeared ill-defined (black arrowhead). Bar: 200 nm. (F) TbNUP98-GFP (white) was used as a marker of the NPC and in control cells displayed as puncta around the nuclear periphery (top panel). In NUP-1 RNAi cells, NPCs clustered in distinct regions of the nuclear periphery (bottom panels, serial images along the <i>z</i>-axis). DAPI was used to visualize DNA (blue). Bar: 2 µm.</p>", "links"=>[], "tags"=>["morphology", "npc", "positioning"], "article_id"=>333216, "categories"=>["Genetics", "Evolutionary Biology"], "users"=>["Kelly N. DuBois", "Sam Alsford", "Jennifer M. Holden", "Johanna Buisson", "Michal Swiderski", "Jean-Mathieu Bart", "Alexander V. Ratushny", "Yakun Wan", "Philippe Bastin", "J. David Barry", "Miguel Navarro", "David Horn", "John D. Aitchison", "Michael P. Rout", "Mark C. Field"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1001287.g005", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Nuclear_morphology_and_NPC_positioning_are_dependent_on_NUP_1_/333216", "title"=>"Nuclear morphology and NPC positioning are dependent on NUP-1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-03-27 00:53:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/662271"], "description"=>"<p>A single NUP-1 orthologue is present in each trypanosomatid genome. Bars represent N- and C-terminal domains, cylinders α-helical repeats. Numbers above domains denote the number of amino acid residues predicted in each domain. Repeat number and length and total protein length in amino acids (aa) are also indicated.</p>", "links"=>[], "tags"=>["coiled", "coil", "restricted"], "article_id"=>332739, "categories"=>["Genetics", "Evolutionary Biology"], "users"=>["Kelly N. DuBois", "Sam Alsford", "Jennifer M. Holden", "Johanna Buisson", "Michal Swiderski", "Jean-Mathieu Bart", "Alexander V. Ratushny", "Yakun Wan", "Philippe Bastin", "J. David Barry", "Miguel Navarro", "David Horn", "John D. Aitchison", "Michael P. Rout", "Mark C. Field"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1001287.g001", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_NUP_1_is_a_large_coiled_coil_protein_identified_in_T_brucei_and_restricted_to_trypanosomatids_/332739", "title"=>"NUP-1 is a large coiled coil protein identified in <i>T. brucei</i> and restricted to trypanosomatids.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-03-27 00:45:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/662461"], "description"=>"<p>(A) Top: Schematic showing NUP-1 in either a compact (left) or an extended (right) conformation. Repeats are shown in red, and the GFP-tagged C-terminal domain is in green. The untagged N-terminus is white. Predicted staining patterns for each conformer are shown below the schematics. Lower: Cells expressing NUP-1-GFP were probed with anti-GFP (green) and anti-NUP-1 repeat region antibody (red) and imaged by confocal microscopy. A montage of 15 confocal <i>z</i>-stack slices through the nucleus of a single trypanosome is shown. There is clear discrimination between the red and green channels, consistent with an extended conformation. (B) Top: Cells expressing NUP-1-GFP were probed with an anti-GFP (green) antibody and a secondary antibody that was immunoabsorbed to eliminate cross-species reactivity and anti-NUP-1 repeat region antibody (red) and imaged by confocal microscopy. Shown is a central slice across the <i>z</i>-axis. DAPI was used to visualise DNA. Bar: 2 µm for all panels. In independent stains using either the anti-GFP or repeat region antibodies alone, it was clear that there was no cross-reactivity (unpublished data). Lower: Cells expressing TbNup98-GFP were probed with anti-GFP (green) and anti-NUP-1 repeat region antibody (red) and imaged by confocal microscopy. Shown is the central slice along the <i>z</i>-axis, also demonstrating clear separation of the nuclear pore complex from the NUP-1 repeat staining. (C) Cells expressing TbNup89-GFP were probed with anti-GFP (green) and an anti-FG repeat antibody (red) to visualize the NPCs and imaged by confocal microscopy. There is clear high correspondence between the red and green stains, consistent with previous data <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001287#pbio.1001287-DeGrasse1\" target=\"_blank\">[36]</a>.</p>", "links"=>[], "tags"=>["arranged"], "article_id"=>332936, "categories"=>["Genetics", "Evolutionary Biology"], "users"=>["Kelly N. DuBois", "Sam Alsford", "Jennifer M. Holden", "Johanna Buisson", "Michal Swiderski", "Jean-Mathieu Bart", "Alexander V. Ratushny", "Yakun Wan", "Philippe Bastin", "J. David Barry", "Miguel Navarro", "David Horn", "John D. Aitchison", "Michael P. Rout", "Mark C. Field"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1001287.g003", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_NUP_1_is_arranged_in_an_extended_conformation_/332936", "title"=>"NUP-1 is arranged in an extended conformation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-03-27 00:48:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/662359"], "description"=>"<p>(A) Fixed PCF cells expressing NUP-1-GFP (white) or BSF cells probed with an anti-NUP-1 antibody (white) were imaged by confocal microscopy. Shown are optical sections of the edge and centre of nuclei taken from image series along the <i>z</i>-axis. DAPI was used to visualize the DNA (blue). Bar: 2 µm. Arrowheads at right panel indicate two putative puncta (i.e., foci of NUP-1 reactivity from which linear regions of NUP-1 reactivity emanate). See also <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001287#pbio.1001287.s006\" target=\"_blank\">Movies S1</a>A–D. (B) FRAP of NUP-1-GFP. After bleaching a portion of the nucleus, no fluorescence recovery was observed during 150 s. See also <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001287#pbio.1001287.s010\" target=\"_blank\">Movie S5</a>. (C) Top: Cells were probed with an anti-NUP-1 antibody (red) and FISH for telomeres (green). DAPI was used to visualize DNA. Nuclei of dividing cells were sectioned into proximal and distal halves for analysis based on the position of telomeres. Bar: 2 µm. Bottom: Fluorescence intensity in confocal nuclear sections of 21 dividing nuclei was recorded for NUP-1, telomeres, and DAPI and plotted as a fraction of the total fluorescence in each nucleus. Means and standard deviations (SD) are shown. The Student's paired <i>t</i> test was used to determine the <i>p</i> value of the fluorescence difference in the proximal compared to the distal half of the nucleus. NS, not significant. (D) Distance between NUP-1 puncta was measured using Metamorph software in cells that were in interphase (1K1N) or early or late mitotic (2K1N). Diagram at top illustrates the measurements taken, essentially of the inter-puncta distance (red). A total of 300 cells were analysed, and the statistical significance calculated using a Student's paired <i>t</i> test (<i>p</i>>0.01).</p>", "links"=>[], "tags"=>["localizes", "periphery"], "article_id"=>332835, "categories"=>["Genetics", "Evolutionary Biology"], "users"=>["Kelly N. DuBois", "Sam Alsford", "Jennifer M. Holden", "Johanna Buisson", "Michal Swiderski", "Jean-Mathieu Bart", "Alexander V. Ratushny", "Yakun Wan", "Philippe Bastin", "J. David Barry", "Miguel Navarro", "David Horn", "John D. Aitchison", "Michael P. Rout", "Mark C. Field"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1001287.g002", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_NUP_1_localizes_to_a_stable_network_around_the_periphery_of_the_nucleus_/332835", "title"=>"NUP-1 localizes to a stable network around the periphery of the nucleus.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-03-27 00:47:15"}
  • {"files"=>["https://ndownloader.figshare.com/files/663231"], "description"=>"<p>(A) Top: NUP-1 RNAi was induced in cells with a <i>GFP:NPT</i> reporter downstream of the repressed <i>VSG 2</i> expression site promoter. Bottom: Expression of <i>VSG 2</i> and the reporter increased as determined by qRT-PCR normalized to Rab11. (B) Top: NUP-1 RNAi was induced in a cell line with <i>NPT</i> reporter ∼2 kb upstream of a <i>de novo</i> telomere. Bottom: Expression site <i>VSG</i> gene expression increased but <i>NPT</i> expression did not as determined by qRT-PCR normalized to Rab11. Expression of <i>VSG 2</i>, the active <i>VSG</i> in this cell line, decreased.</p>", "links"=>[], "tags"=>["nup-1", "derepresses", "telomeric"], "article_id"=>333698, "categories"=>["Genetics", "Evolutionary Biology"], "users"=>["Kelly N. DuBois", "Sam Alsford", "Jennifer M. Holden", "Johanna Buisson", "Michal Swiderski", "Jean-Mathieu Bart", "Alexander V. Ratushny", "Yakun Wan", "Philippe Bastin", "J. David Barry", "Miguel Navarro", "David Horn", "John D. Aitchison", "Michael P. Rout", "Mark C. Field"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1001287.g009", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Depletion_of_NUP_1_derepresses_the_entire_expression_site_but_not_all_telomeric_genes_/333698", "title"=>"Depletion of NUP-1 derepresses the entire expression site, but not all telomeric genes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-03-27 01:01:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/662874"], "description"=>"<p>(A) Cells were probed with an anti-NUP-1 antibody (red) and FISH for telomeres (green). DAPI was used to visualize DNA (blue). NUP-1 and telomeres are in close contact throughout the cell cycle. (B) Telomeres (green) were observed in nuclear blebs (white arrows). An anti-NOG-1 antibody (red) was used to visualize the nucleolus and DAPI for DNA (blue). (C) FISH for telomeres (green) and minichromosomes (red). Telomeres marked by only green fluorescence are megabase chromosomes. In control cells, (top panel) megabase chromosomes did not condense as far towards the centre of a dividing nucleus as the minichromosomes (white arrow heads). In NUP-1 depleted cells, megabase chromosomes co-localized with nuclear blebs (white arrows). DAPI was used to visualize DNA (blue). Bar: 2 µm for all panels.</p>", "links"=>[], "tags"=>["genetics and genomics", "Evolutionary biology"], "article_id"=>333346, "categories"=>["Genetics", "Evolutionary Biology"], "users"=>["Kelly N. DuBois", "Sam Alsford", "Jennifer M. Holden", "Johanna Buisson", "Michal Swiderski", "Jean-Mathieu Bart", "Alexander V. Ratushny", "Yakun Wan", "Philippe Bastin", "J. David Barry", "Miguel Navarro", "David Horn", "John D. Aitchison", "Michael P. Rout", "Mark C. Field"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1001287.g006", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Chromatin_organization_is_dependent_on_NUP_1_/333346", "title"=>"Chromatin organization is dependent on NUP-1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-03-27 00:55:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/663108"], "description"=>"<p>(A) Heatmap for microarray following NUP-1 knockdown in BSF cells. Of 8,110 genes, 62 were increased in relative expression greater than 2-fold. Yellow and blue represent upregulation and downregulation, respectively, and black no expression change. Gene accession numbers/annotation are given for the upregulated genes. (B) qRT-PCR quantitation of the expression of <i>EP</i> and <i>GPEET procyclin</i> genes (left panel), selected ES <i>VSG</i> genes (middle panel), and housekeeping genes (right panel) during NUP-1 RNAi. The expression level in control cells was set to one, and <i>y</i>-axis is the relative expression compared to control cells, normalized to β-tubulin.</p>", "links"=>[], "tags"=>["depletion", "causes", "developmentally", "regulated", "genes", "bsf"], "article_id"=>333578, "categories"=>["Genetics", "Evolutionary Biology"], "users"=>["Kelly N. DuBois", "Sam Alsford", "Jennifer M. Holden", "Johanna Buisson", "Michal Swiderski", "Jean-Mathieu Bart", "Alexander V. Ratushny", "Yakun Wan", "Philippe Bastin", "J. David Barry", "Miguel Navarro", "David Horn", "John D. Aitchison", "Michael P. Rout", "Mark C. Field"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1001287.g008", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_NUP_1_depletion_causes_increased_expression_of_developmentally_regulated_genes_in_BSF_cells_/333578", "title"=>"NUP-1 depletion causes increased expression of developmentally regulated genes in BSF cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-03-27 00:59:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/662990"], "description"=>"<p>(A) eGFP reporter modification of the telomeric MVSG 1.22 locus in PCF cells. P, promoter; BSD, blasticidin<sup>R</sup> gene; Pol I, RNA polymerase l. (B) NUP-1 RNAi induction causes time-dependent increase in <i>MVSG</i> and eGFP expression. All values are fold expression compared to uninduced cells determined by qRT-PCR and normalized to β-tubulin. Clathrin, PLK, and ATPaseAF; RNA was isolated after manifestation of the proliferative/morphological phenotype.</p>", "links"=>[], "tags"=>["depletion", "causes", "misregulation", "metacyclic", "genes", "pcf"], "article_id"=>333464, "categories"=>["Genetics", "Evolutionary Biology"], "users"=>["Kelly N. DuBois", "Sam Alsford", "Jennifer M. Holden", "Johanna Buisson", "Michal Swiderski", "Jean-Mathieu Bart", "Alexander V. Ratushny", "Yakun Wan", "Philippe Bastin", "J. David Barry", "Miguel Navarro", "David Horn", "John D. Aitchison", "Michael P. Rout", "Mark C. Field"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1001287.g007", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_NUP_1_depletion_causes_misregulation_of_metacyclic_VSG_MVSG_genes_in_PCF_cells_/333464", "title"=>"NUP-1 depletion causes misregulation of metacyclic <i>VSG</i> (<i>MVSG</i>) genes in PCF cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-03-27 00:57:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/662607"], "description"=>"<p>NUP-1 RNAi was induced in both BSF and PCF cells. (A, B) NUP-1 mRNA was depleted by ∼30% as measured by qRT-PCR normalized to β-tubulin, corresponding to a ∼75% decrease in NUP-1 protein after 24 h of RNAi induction. Western bands were quantified, normalized to BIP levels, and represented as bar graphs. Error bars are the result of two experimental replicates. (C) IFA using DAPI to visualize DNA (blue). Nuclei of control cells were ovoid with well-defined boundaries, and induced cells exhibited nuclei with diffuse boundaries (i.e., rather than the DAPI nuclear signal being sharply demarcated, the signal gradually decreases with distance from the nuclear centre), and abnormal protrusions (blebs) in both BSF (left panel) and PCF (right panel) cells are seen. Bar: 5 µm. (D) Nuclei with abnormal extensions (blebbing) and with diffuse boundaries were examined over a time course of NUP-1 RNAi induction in BSF cells. 200 cells were scored for each time point. Percentage of nuclei with blebbing or diffuse phenotypes is shown, with the remainder of cells demonstrating a normal phenotype. Initially an increase in cells with bleb nuclei (black) was observed, followed by a decrease in bleb nuclei and an increase in cells with diffuse nuclei (gray). Note that these features are present at less than 1% in an uninduced population. Bar: 2 µm. (E) NUP-1 knockdown BSF cells were probed with an anti-NUP-1 antibody (white). DAPI is used to visualize DNA (blue). Shown are serial sections along the <i>z</i>-axis. Bar: 2 µm.</p>", "links"=>[], "tags"=>["genetics and genomics", "Evolutionary biology"], "article_id"=>333074, "categories"=>["Genetics", "Evolutionary Biology"], "users"=>["Kelly N. DuBois", "Sam Alsford", "Jennifer M. Holden", "Johanna Buisson", "Michal Swiderski", "Jean-Mathieu Bart", "Alexander V. Ratushny", "Yakun Wan", "Philippe Bastin", "J. David Barry", "Miguel Navarro", "David Horn", "John D. Aitchison", "Michael P. Rout", "Mark C. Field"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1001287.g004", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_NUP_1_is_necessary_for_cell_growth_and_maintenance_of_nuclear_architecture_/333074", "title"=>"NUP-1 is necessary for cell growth and maintenance of nuclear architecture.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-03-27 00:51:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/663338"], "description"=>"<p>(A) Top: Cells were probed with antibodies against the active <i>VSG 2</i> (red) and an alternate <i>VSG 6</i> (green) following 96 h of NUP-1 RNAi induction. Cells switched from expressing <i>VSG 2</i> to <i>VSG 6</i> (white arrow), expressed neither <i>VSG 2</i> nor <i>VSG 6</i> (white arrowhead), or expressed both (not shown). Bar: 10 µm. Bottom: Quantitation of increased <i>VSG</i> switching in NUP-1 knockdown cells. Graph represents average and standard deviation of three independent NUP-1 RNAi lines (<i>n</i> = 1,000 cells). (B) NUP-1 depletion represses differentiation-induced repositioning of the active expression site to the nuclear periphery. Left: Representative images of maximum intensity projections of 3-D data sets of the GFP-LacI tagged active expression site (green) in wild type cells or NUP-1 RNAi cells (induced for 24 h) after induction of differentiation. Bar: 1 µm. Right: Statistical analysis of the expression site position. The active expression site rapidly repositions to the nuclear periphery in wild type cells. Repositioning is significantly inhibited in NUP-1 RNAi cells. Graph represents the average of three replicate experiments.</p>", "links"=>[], "tags"=>["knockdown", "leads", "vsg", "switching", "represses", "differentiation-induced"], "article_id"=>333796, "categories"=>["Genetics", "Evolutionary Biology"], "users"=>["Kelly N. DuBois", "Sam Alsford", "Jennifer M. Holden", "Johanna Buisson", "Michal Swiderski", "Jean-Mathieu Bart", "Alexander V. Ratushny", "Yakun Wan", "Philippe Bastin", "J. David Barry", "Miguel Navarro", "David Horn", "John D. Aitchison", "Michael P. Rout", "Mark C. Field"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1001287.g010", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_NUP_1_knockdown_leads_to_increased_VSG_switching_and_represses_differentiation_induced_expression_site_repositioning_/333796", "title"=>"NUP-1 knockdown leads to increased VSG switching and represses differentiation-induced expression site repositioning.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-03-27 01:03:16"}
  • {"files"=>["https://ndownloader.figshare.com/files/340379", "https://ndownloader.figshare.com/files/340625", "https://ndownloader.figshare.com/files/340689", "https://ndownloader.figshare.com/files/340781", "https://ndownloader.figshare.com/files/340853", "https://ndownloader.figshare.com/files/340906", "https://ndownloader.figshare.com/files/340961", "https://ndownloader.figshare.com/files/341016", "https://ndownloader.figshare.com/files/341078", "https://ndownloader.figshare.com/files/341122", "https://ndownloader.figshare.com/files/341166", "https://ndownloader.figshare.com/files/341210", "https://ndownloader.figshare.com/files/341240", "https://ndownloader.figshare.com/files/341281"], "description"=>"<div><p>A unifying feature of eukaryotic nuclear organization is genome segregation into transcriptionally active euchromatin and transcriptionally repressed heterochromatin. In metazoa, lamin proteins preserve nuclear integrity and higher order heterochromatin organization at the nuclear periphery, but no non-metazoan lamin orthologues have been identified, despite the likely presence of nucleoskeletal elements in many lineages. This suggests a metazoan-specific origin for lamins, and therefore that distinct protein elements must compose the nucleoskeleton in other lineages. The trypanosomatids are highly divergent organisms and possess well-documented but remarkably distinct mechanisms for control of gene expression, including polycistronic transcription and <em>trans-</em>splicing. NUP-1 is a large protein localizing to the nuclear periphery of <em>Trypanosoma brucei</em> and a candidate nucleoskeletal component. We sought to determine if NUP-1 mediates heterochromatin organization and gene regulation at the nuclear periphery by examining the influence of NUP-1 knockdown on morphology, chromatin positioning, and transcription. We demonstrate that NUP-1 is essential and part of a stable network at the inner face of the trypanosome nuclear envelope, since knockdown cells have abnormally shaped nuclei with compromised structural integrity. NUP-1 knockdown also disrupts organization of nuclear pore complexes and chromosomes. Most significantly, we find that NUP-1 is required to maintain the silenced state of developmentally regulated genes at the nuclear periphery; NUP-1 knockdown results in highly specific mis-regulation of telomere-proximal silenced variant surface glycoprotein (<em>VSG</em>) expression sites and procyclin loci, indicating a disruption to normal chromatin organization essential to life-cycle progression. Further, NUP-1 depletion leads to increased <em>VSG</em> switching and therefore appears to have a role in control of antigenic variation. Thus, analogous to vertebrate lamins, NUP-1 is a major component of the nucleoskeleton with key roles in organization of the nuclear periphery, heterochromatin, and epigenetic control of developmentally regulated loci.</p> </div>", "links"=>[], "tags"=>["nup-1", "coiled-coil", "nucleoskeletal", "trypanosomes", "lamin-like", "functions"], "article_id"=>127282, "categories"=>["Genetics", "Evolutionary Biology"], "users"=>["Kelly N. DuBois", "Sam Alsford", "Jennifer M. Holden", "Johanna Buisson", "Michal Swiderski", "Jean-Mathieu Bart", "Alexander V. Ratushny", "Yakun Wan", "Philippe Bastin", "J. David Barry", "Miguel Navarro", "David Horn", "John D. Aitchison", "Michael P. Rout", "Mark C. Field"], "doi"=>["https://dx.doi.org/10.1371/journal.pbio.1001287.s001", "https://dx.doi.org/10.1371/journal.pbio.1001287.s002", "https://dx.doi.org/10.1371/journal.pbio.1001287.s003", "https://dx.doi.org/10.1371/journal.pbio.1001287.s004", "https://dx.doi.org/10.1371/journal.pbio.1001287.s005", "https://dx.doi.org/10.1371/journal.pbio.1001287.s006", "https://dx.doi.org/10.1371/journal.pbio.1001287.s007", "https://dx.doi.org/10.1371/journal.pbio.1001287.s008", "https://dx.doi.org/10.1371/journal.pbio.1001287.s009", "https://dx.doi.org/10.1371/journal.pbio.1001287.s010", "https://dx.doi.org/10.1371/journal.pbio.1001287.s011", "https://dx.doi.org/10.1371/journal.pbio.1001287.s012", "https://dx.doi.org/10.1371/journal.pbio.1001287.s013", "https://dx.doi.org/10.1371/journal.pbio.1001287.s014"], "stats"=>{"downloads"=>2, "page_views"=>22, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/NUP_1_Is_a_Large_Coiled_Coil_Nucleoskeletal_Protein_in_Trypanosomes_with_Lamin_Like_Functions/127282", "title"=>"NUP-1 Is a Large Coiled-Coil Nucleoskeletal Protein in Trypanosomes with Lamin-Like Functions", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-03-27 02:01:22"}

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  • {"unique-ip"=>"30", "full-text"=>"28", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}

Relative Metric

{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[316, 541, 663, 766, 856, 950, 1041, 1128, 1218, 1302, 1382, 1456, 1526, 1593, 1657, 1729, 1796, 1862, 1930, 1999, 2065, 2132, 2202, 2261, 2319]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[319, 556, 679, 785, 881, 970, 1062, 1149, 1236, 1323, 1402, 1474, 1545, 1617, 1681, 1754, 1822, 1892, 1963, 2031, 2099, 2165, 2233, 2299, 2359]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[333, 576, 707, 814, 908, 1004, 1104, 1197, 1280, 1370, 1449, 1531, 1603, 1673, 1742, 1817, 1886, 1954, 2025, 2098, 2171, 2234, 2304, 2365, 2431]}]}
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