Coronin 1 Regulates Cognition and Behavior through Modulation of cAMP/Protein Kinase A Signaling
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{"title"=>"Coronin 1 Regulates Cognition and Behavior through Modulation of cAMP/Protein Kinase A Signaling", "type"=>"journal", "authors"=>[{"first_name"=>"Rajesh", "last_name"=>"Jayachandran", "scopus_author_id"=>"6603019392"}, {"first_name"=>"Xiaolong", "last_name"=>"Liu", "scopus_author_id"=>"53873013700"}, {"first_name"=>"Somdeb", "last_name"=>"BoseDasgupta", "scopus_author_id"=>"23032977600"}, {"first_name"=>"Philipp", "last_name"=>"Müller", "scopus_author_id"=>"7403138678"}, {"first_name"=>"Chun Lei", "last_name"=>"Zhang", "scopus_author_id"=>"55920776400"}, {"first_name"=>"Despina", "last_name"=>"Moshous", "scopus_author_id"=>"6603167051"}, {"first_name"=>"Vera", "last_name"=>"Studer", "scopus_author_id"=>"56083236700"}, {"first_name"=>"Jacques", "last_name"=>"Schneider", "scopus_author_id"=>"7404422481"}, {"first_name"=>"Christel", "last_name"=>"Genoud", "scopus_author_id"=>"6507674239"}, {"first_name"=>"Catherine", "last_name"=>"Fossoud", "scopus_author_id"=>"6506937941"}, {"first_name"=>"Frédéric", "last_name"=>"Gambino", "scopus_author_id"=>"18037201400"}, {"first_name"=>"Malik", "last_name"=>"Khelfaoui", "scopus_author_id"=>"8600266700"}, {"first_name"=>"Christian", "last_name"=>"Müller", "scopus_author_id"=>"7404109791"}, {"first_name"=>"Deborah", "last_name"=>"Bartholdi", "scopus_author_id"=>"6602960364"}, {"first_name"=>"Helene", "last_name"=>"Rossez", "scopus_author_id"=>"55612514500"}, {"first_name"=>"Michael", "last_name"=>"Stiess", "scopus_author_id"=>"8868114800"}, {"first_name"=>"Xander", "last_name"=>"Houbaert", "scopus_author_id"=>"55830605200"}, {"first_name"=>"Rolf", "last_name"=>"Jaussi", "scopus_author_id"=>"55927698900"}, {"first_name"=>"Daniel", "last_name"=>"Frey", "scopus_author_id"=>"57196951529"}, {"first_name"=>"Richard A.", "last_name"=>"Kammerer", "scopus_author_id"=>"7005163423"}, {"first_name"=>"Xavier", "last_name"=>"Deupi", "scopus_author_id"=>"6507252395"}, {"first_name"=>"Jean Pierre", "last_name"=>"de Villartay", "scopus_author_id"=>"7005443414"}, {"first_name"=>"Andreas", "last_name"=>"Lüthi", "scopus_author_id"=>"7004830057"}, {"first_name"=>"Yann", "last_name"=>"Humeau", "scopus_author_id"=>"6506835486"}, {"first_name"=>"Jean", "last_name"=>"Pieters", "scopus_author_id"=>"7006329030"}], "year"=>2014, "source"=>"PLoS Biology", "identifiers"=>{"isbn"=>"1545-7885 (Electronic)\\r1544-9173 (Linking)", "sgr"=>"84899016569", "pui"=>"372909834", "doi"=>"10.1371/journal.pbio.1001820", "pmid"=>"24667537", "issn"=>"15457885", "scopus"=>"2-s2.0-84899016569"}, "id"=>"334732ce-ece8-3931-921d-afcffb8daf31", "abstract"=>"Cognitive and behavioral disorders are thought to be a result of neuronal dysfunction, but the underlying molecular defects remain largely unknown. An important signaling pathway involved in the regulation of neuronal function is the cyclic AMP/Protein kinase A pathway. We here show an essential role for coronin 1, which is encoded in a genomic region associated with neurobehavioral dysfunction, in the modulation of cyclic AMP/PKA signaling. We found that coronin 1 is specifically expressed in excitatory but not inhibitory neurons and that coronin 1 deficiency results in loss of excitatory synapses and severe neurobehavioral disabilities, including reduced anxiety, social deficits, increased aggression, and learning defects. Electrophysiological analysis of excitatory synaptic transmission in amygdala revealed that coronin 1 was essential for cyclic-AMP-protein kinase A-dependent presynaptic plasticity. We further show that upon cell surface stimulation, coronin 1 interacted with the G protein subtype Gαs to stimulate the cAMP/PKA pathway. The absence of coronin 1 or expression of coronin 1 mutants unable to interact with Gαs resulted in a marked reduction in cAMP signaling. Strikingly, synaptic plasticity and behavioral defects of coronin 1-deficient mice were restored by in vivo infusion of a membrane-permeable cAMP analogue. Together these results identify coronin 1 as being important for cognition and behavior through its activity in promoting cAMP/PKA-dependent synaptic plasticity and may open novel avenues for the dissection of signal transduction pathways involved in neurobehavioral processes.", "link"=>"http://www.mendeley.com/research/coronin-1-regulates-cognition-behavior-through-modulation-campprotein-kinase-signaling", "reader_count"=>67, "reader_count_by_academic_status"=>{"Unspecified"=>5, "Professor > Associate Professor"=>3, "Librarian"=>1, "Student > Doctoral Student"=>3, "Researcher"=>13, "Student > Ph. D. Student"=>20, "Student > Postgraduate"=>2, "Other"=>3, "Student > Master"=>11, "Student > Bachelor"=>3, "Lecturer > Senior Lecturer"=>1, "Professor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>5, "Professor > Associate Professor"=>3, "Librarian"=>1, "Student > Doctoral Student"=>3, "Researcher"=>13, "Student > Ph. D. Student"=>20, "Student > Postgraduate"=>2, "Other"=>3, "Student > Master"=>11, "Student > Bachelor"=>3, "Lecturer > Senior Lecturer"=>1, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>6, "Agricultural and Biological Sciences"=>26, "Veterinary Science and Veterinary Medicine"=>1, "Chemical Engineering"=>1, "Chemistry"=>1, "Biochemistry, Genetics and Molecular Biology"=>8, "Medicine and Dentistry"=>11, "Neuroscience"=>6, "Design"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>2, "Psychology"=>2, "Social Sciences"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>11}, "Social Sciences"=>{"Social Sciences"=>1}, "Psychology"=>{"Psychology"=>2}, "Unspecified"=>{"Unspecified"=>6}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>2}, "Chemical Engineering"=>{"Chemical Engineering"=>1}, "Design"=>{"Design"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Neuroscience"=>{"Neuroscience"=>6}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>26}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>8}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "reader_count_by_country"=>{"France"=>1, "Germany"=>3, "India"=>1}, "group_count"=>2}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1434948"], "description"=>"<p>(A) Hippocampal neurons (7 d culture) were fixed and stained with antibodies against coronin 1 (Alexa 488) and cAMP-, PKA substrate-, or P-CREB antibodies (568). Scale bar, 20 µm. (B) Wild-type or coronin 1–deficient brain lysates were immunoblotted using anti-P-CREB antibodies and reprobed using anti–coronin 1 and anti-actin antibodies. (C) Sections from wild-type or coronin 1–deficient brain were immunolabeled using anti–P-CREB antibodies, anti–coronin 1, and anti-histone antibodies. Scale bar, 10 µm. (D) Amygdalary regions of wild-type and coronin 1–deficient mice were analyzed for cAMP levels by ELISA. The values shown are normalized to total protein amounts (pmol cAMP/mg total protein). The data shown are from three independent experiments; <i>n</i> = 10 mice per genotype, <i>p</i><0.01, Student's <i>t</i> test, see <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001820#pbio.1001820.s018\" target=\"_blank\">Table S1</a>. (E) Basal (Control) and cAMP-stimulated PKA activity was determined in brain lysates from wild-type (WT) and coronin 1–deficient (KO) mice using a Peptag assay. PKA activity was monitored by the negative charge increase of the PKA substrate-containing peptide. (Upper panel) Electrophoretic pattern. (Lower panel) Quantitation (<i>n</i> = 3), <i>p</i> = 0.01. (F, G) Ventricle sizes in the presence and absence of coronin 1 as analyzed by MRI on live animals. H, mean ventricular size. <i>N</i> = 6, left <i>p</i><0.01, right <i>p</i><0.05, and middle <i>p</i><0.01, Student's <i>t</i> test, see <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001820#pbio.1001820.s018\" target=\"_blank\">Table S1</a>.</p>", "links"=>[], "tags"=>["cell biology", "Signal transduction", "cell signaling", "neuroscience", "Molecular neuroscience", "p-creb", "ventricle", "sizes", "coronin"], "article_id"=>974092, "categories"=>["Biological Sciences"], "users"=>["Rajesh Jayachandran", "Xiaolong Liu", "Somdeb BoseDasgupta", "Philipp Müller", "Chun-Lei Zhang", "Despina Moshous", "Vera Studer", "Jacques Schneider", "Christel Genoud", "Catherine Fossoud", "Frédéric Gambino", "Malik Khelfaoui", "Christian Müller", "Deborah Bartholdi", "Helene Rossez", "Michael Stiess", "Xander Houbaert", "Rolf Jaussi", "Daniel Frey", "Richard A. Kammerer", "Xavier Deupi", "Jean-Pierre de Villartay", "Andreas Lüthi", "Yann Humeau", "Jean Pieters"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1001820.g004", "stats"=>{"downloads"=>0, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_cAMP_production_P_CREB_analysis_and_ventricle_sizes_in_the_presence_and_absence_of_coronin_1_/974092", "title"=>"cAMP production, P-CREB analysis, and ventricle sizes in the presence and absence of coronin 1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-25 04:02:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1434938"], "description"=>"<p>(A) Brain regions as indicated were dissected from wild-type or coronin 1–deficient mice, lysed, and immunoblotted for coronin 1 (upper panel) or actin (lower panel). (B) Sections from wild-type mouse brain were analyzed for the expression of coronin 1 (Alexa Fluor 488) and neurofilament-M chain (Alexa Fluor 546 and 568) as well as DRAQ5. Scale bar, 100 µm (Merge); 25 µm (Zoom). (C) Sections from wild-type mouse brain were analyzed for the expression of coronin 1 (Alexa Fluor 488) and neurofilament-M chain (Alexa Fluor 546). Scale bar, 100 µm (merge); 25 µm (zoom). (D) Sections from wild-type mouse brain were analyzed for the expression of coronin 1 (Alexa Fluor 488) and neurofilament-H (Alexa Fluor 546). 10× magnification and imaged with a confocal microscope (scale bar, 100 µm). (E) Sections from wild-type mouse brain (cortex) were labeled with anti-GAD67 (left panels) or anti-Vglut (right panels) as well as anti–coronin 1 antibodies, followed by Alex Fluor 568 or 546 as well as 488 labeled secondary antibodies. Scale bar, 25 µm (left) and 10 µM (right). (F) Hippocampal neurons were labeled using ani-coronin 1 antibodies and double labeled using either anti-vGLUT1 or anti-vGAT antibodies, followed by Alex Fluor 568 (for coronin 1) or 488 (for Vglut and Vgat) labeled secondary antibodies, respectively. (G) Hippocampal neurons isolated from wild-type mice were labeled using anti–synapsin 1 and anti–coronin 1 antibodies, followed by Alex Fluor 488 or 568 labeled secondary antibodies, respectively. Scale bar, 10 µm.</p>", "links"=>[], "tags"=>["cell biology", "Signal transduction", "cell signaling", "neuroscience", "Molecular neuroscience", "localization", "excitatory", "inhibitory"], "article_id"=>974089, "categories"=>["Biological Sciences"], "users"=>["Rajesh Jayachandran", "Xiaolong Liu", "Somdeb BoseDasgupta", "Philipp Müller", "Chun-Lei Zhang", "Despina Moshous", "Vera Studer", "Jacques Schneider", "Christel Genoud", "Catherine Fossoud", "Frédéric Gambino", "Malik Khelfaoui", "Christian Müller", "Deborah Bartholdi", "Helene Rossez", "Michael Stiess", "Xander Houbaert", "Rolf Jaussi", "Daniel Frey", "Richard A. Kammerer", "Xavier Deupi", "Jean-Pierre de Villartay", "Andreas Lüthi", "Yann Humeau", "Jean Pieters"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1001820.g002", "stats"=>{"downloads"=>1, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Coronin_1_distribution_and_localization_in_excitatory_and_inhibitory_neurons_/974089", "title"=>"Coronin 1 distribution and localization in excitatory and inhibitory neurons.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-25 04:02:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1434937"], "description"=>"<p>(A) Resident intruder aggression analysis. The mouse that initiated the fight and displayed dominance was scored as the winner. <i>N</i> = 37 trials, <i>p</i><0.01. (B) Tube displacement test for social dominance analysis. The first mouse to exit the tube was scored the loser and the other the winner (socially dominant). <i>N</i> = 33 trials, <i>p</i><0.05. (C). Light versus dark preference. <i>N</i> = 14 WT and 16 coronin 1 −/−, <i>p</i><0.001, Student's <i>t</i> test. (D) Elevated plus maze analysis (<i>n</i> = 16 wild-type and 15 coronin 1 −/−). Open arm, <i>p</i><0.05; closed arm, <i>p</i> = 0.01. (E) The number of calls emitted in ultrasonic frequencies. <i>n</i> = 15 wild-type and 13 coronin 1 −/−, <i>p</i><0.01 for 40–60 kHz and <i>p</i><0.01 for >60 kHz, Student's <i>t</i> test. (F) For self-grooming, the duration (<i>p</i><0.01, left) and number of times (<i>p</i><0.0001, right) was scored. <i>n</i> = 13 per genotype. (G) Twenty-four hours after cued fear conditioning, coronin 1–deficient mice exhibit impaired long-term memory as indicated by reduced freezing levels during CS+ exposure. Wild-type, <i>n</i> = 12 and coronin 1 −/−, <i>n</i> = 11 mice. <i>p</i><0.01, RM-ANOVA. Freezing during CS− exposure or in the absence of auditory stimulation was not different. (H) Mean contextual freezing during a 4 min context exposure before and 24 h after contextual fear conditioning. Twenty-four hours after contextual fear conditioning, coronin 1 −/− mice exhibit impaired long-term memory as indicated by reduced freezing levels compared to wild-type mice. <i>p</i><0.01, RM-ANOVA. <i>N</i> = 5 WT and 6 coronin 1 −/− mice. (I, J) Three chamber analysis. Coronin 1 −/− mice show a reduced sociability in session 1 (I) and a reduced social novelty in session 2 (J) relative to wild-type mice. <i>n</i> = 16 wild-type and 16 coronin 1–deficient mice. **<i>p</i><0.01 and ***<i>p</i><0.001, RM-ANOVA, see <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001820#pbio.1001820.s018\" target=\"_blank\">Table S1</a> for additional statistics. Wild type: gray bars; coronin 1-deficient: red bars.</p>", "links"=>[], "tags"=>["cell biology", "Signal transduction", "cell signaling", "neuroscience", "Molecular neuroscience", "impaired", "socialization", "coronin"], "article_id"=>974088, "categories"=>["Biological Sciences"], "users"=>["Rajesh Jayachandran", "Xiaolong Liu", "Somdeb BoseDasgupta", "Philipp Müller", "Chun-Lei Zhang", "Despina Moshous", "Vera Studer", "Jacques Schneider", "Christel Genoud", "Catherine Fossoud", "Frédéric Gambino", "Malik Khelfaoui", "Christian Müller", "Deborah Bartholdi", "Helene Rossez", "Michael Stiess", "Xander Houbaert", "Rolf Jaussi", "Daniel Frey", "Richard A. Kammerer", "Xavier Deupi", "Jean-Pierre de Villartay", "Andreas Lüthi", "Yann Humeau", "Jean Pieters"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1001820.g001", "stats"=>{"downloads"=>0, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Increased_aggression_and_impaired_memory_and_socialization_in_the_absence_of_coronin_1_/974088", "title"=>"Increased aggression and impaired memory and socialization in the absence of coronin 1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-25 04:02:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1435009", "https://ndownloader.figshare.com/files/1435010", "https://ndownloader.figshare.com/files/1435011", "https://ndownloader.figshare.com/files/1435012", "https://ndownloader.figshare.com/files/1435013", "https://ndownloader.figshare.com/files/1435014", "https://ndownloader.figshare.com/files/1435015", "https://ndownloader.figshare.com/files/1435016", "https://ndownloader.figshare.com/files/1435018", "https://ndownloader.figshare.com/files/1435019", "https://ndownloader.figshare.com/files/1435020", "https://ndownloader.figshare.com/files/1435021", "https://ndownloader.figshare.com/files/1435022", "https://ndownloader.figshare.com/files/1435023", "https://ndownloader.figshare.com/files/1435024", "https://ndownloader.figshare.com/files/1435026", "https://ndownloader.figshare.com/files/1435027", "https://ndownloader.figshare.com/files/1435028", "https://ndownloader.figshare.com/files/1435029", "https://ndownloader.figshare.com/files/1435030", "https://ndownloader.figshare.com/files/1435031", "https://ndownloader.figshare.com/files/1435032"], "description"=>"<div><p>Cognitive and behavioral disorders are thought to be a result of neuronal dysfunction, but the underlying molecular defects remain largely unknown. An important signaling pathway involved in the regulation of neuronal function is the cyclic AMP/Protein kinase A pathway. We here show an essential role for coronin 1, which is encoded in a genomic region associated with neurobehavioral dysfunction, in the modulation of cyclic AMP/PKA signaling. We found that coronin 1 is specifically expressed in excitatory but not inhibitory neurons and that coronin 1 deficiency results in loss of excitatory synapses and severe neurobehavioral disabilities, including reduced anxiety, social deficits, increased aggression, and learning defects. Electrophysiological analysis of excitatory synaptic transmission in amygdala revealed that coronin 1 was essential for cyclic–AMP–protein kinase A–dependent presynaptic plasticity. We further show that upon cell surface stimulation, coronin 1 interacted with the G protein subtype Gαs to stimulate the cAMP/PKA pathway. The absence of coronin 1 or expression of coronin 1 mutants unable to interact with Gαs resulted in a marked reduction in cAMP signaling. Strikingly, synaptic plasticity and behavioral defects of coronin 1–deficient mice were restored by <i>in vivo</i> infusion of a membrane-permeable cAMP analogue. Together these results identify coronin 1 as being important for cognition and behavior through its activity in promoting cAMP/PKA-dependent synaptic plasticity and may open novel avenues for the dissection of signal transduction pathways involved in neurobehavioral processes.</p></div>", "links"=>[], "tags"=>["cell biology", "Signal transduction", "cell signaling", "neuroscience", "Molecular neuroscience", "regulates", "cognition", "modulation", "kinase"], "article_id"=>974150, "categories"=>["Biological Sciences"], "users"=>["Rajesh Jayachandran", "Xiaolong Liu", "Somdeb BoseDasgupta", "Philipp Müller", "Chun-Lei Zhang", "Despina Moshous", "Vera Studer", "Jacques Schneider", "Christel Genoud", "Catherine Fossoud", "Frédéric Gambino", "Malik Khelfaoui", "Christian Müller", "Deborah Bartholdi", "Helene Rossez", "Michael Stiess", "Xander Houbaert", "Rolf Jaussi", "Daniel Frey", "Richard A. Kammerer", "Xavier Deupi", "Jean-Pierre de Villartay", "Andreas Lüthi", "Yann Humeau", "Jean Pieters"], "doi"=>["https://dx.doi.org/10.1371/journal.pbio.1001820.s001", "https://dx.doi.org/10.1371/journal.pbio.1001820.s002", "https://dx.doi.org/10.1371/journal.pbio.1001820.s003", "https://dx.doi.org/10.1371/journal.pbio.1001820.s004", "https://dx.doi.org/10.1371/journal.pbio.1001820.s005", "https://dx.doi.org/10.1371/journal.pbio.1001820.s006", "https://dx.doi.org/10.1371/journal.pbio.1001820.s007", "https://dx.doi.org/10.1371/journal.pbio.1001820.s008", "https://dx.doi.org/10.1371/journal.pbio.1001820.s009", "https://dx.doi.org/10.1371/journal.pbio.1001820.s010", "https://dx.doi.org/10.1371/journal.pbio.1001820.s011", "https://dx.doi.org/10.1371/journal.pbio.1001820.s012", "https://dx.doi.org/10.1371/journal.pbio.1001820.s013", "https://dx.doi.org/10.1371/journal.pbio.1001820.s014", "https://dx.doi.org/10.1371/journal.pbio.1001820.s015", "https://dx.doi.org/10.1371/journal.pbio.1001820.s016", "https://dx.doi.org/10.1371/journal.pbio.1001820.s017", "https://dx.doi.org/10.1371/journal.pbio.1001820.s018", "https://dx.doi.org/10.1371/journal.pbio.1001820.s019", "https://dx.doi.org/10.1371/journal.pbio.1001820.s020", "https://dx.doi.org/10.1371/journal.pbio.1001820.s021", "https://dx.doi.org/10.1371/journal.pbio.1001820.s022"], "stats"=>{"downloads"=>1, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Coronin_1_Regulates_Cognition_and_Behavior_through_Modulation_of_cAMP_Protein_Kinase_A_Signaling_/974150", "title"=>"Coronin 1 Regulates Cognition and Behavior through Modulation of cAMP/Protein Kinase A Signaling", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-03-25 04:02:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1434953"], "description"=>"<p>(A, B) Coronin 1–expressing Mel JuSo cells were left untreated or stimulated with isoproterenol (10 µM, 10 min), lysed, and Gα molecules were immunoprecipitated with the indicated antibodies as described, followed by separation on SDS-PAGE and immunoblotting using anti–coronin 1 (A) or anti-Gα antibodies (B). The appearance of Gα as a doublet may be a result of posttranslational modifications and/or partial cleavage. (C) Coronin 1–expressing Mel JuSo cells were treated with Latrunculin B (4 µM, 30 min; see <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001820#pbio-1001820-g005\" target=\"_blank\">Figure 5D–F</a>), lysed, and proteins immunoprecipitated using anti-Gα antibodies, followed by SDS-PAGE and immunoblotting. (D) NIE-115 cells were stimulated with isoproterenol (10 µM, 10 min), lysed, and immunoprecipitated using anti–coronin 1 antibodies. Protein complexes were separated by SDS-PAGE and immunoblotted for coronin 1 and the Gα molecules indicated. (E) Coronin 1–expressing Mel JuSo cells or control cells were starved in rolipram (100 µM, 1 h) and stimulated with cholera toxin (1 µg/mL) for 1 h at 37°C prior to stimulation with isoproterenol (10 µM, 4 min). cAMP production was measured as described in <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001820#s4\" target=\"_blank\">Materials and Methods</a>.</p>", "links"=>[], "tags"=>["cell biology", "Signal transduction", "cell signaling", "neuroscience", "Molecular neuroscience", "coronin"], "article_id"=>974097, "categories"=>["Biological Sciences"], "users"=>["Rajesh Jayachandran", "Xiaolong Liu", "Somdeb BoseDasgupta", "Philipp Müller", "Chun-Lei Zhang", "Despina Moshous", "Vera Studer", "Jacques Schneider", "Christel Genoud", "Catherine Fossoud", "Frédéric Gambino", "Malik Khelfaoui", "Christian Müller", "Deborah Bartholdi", "Helene Rossez", "Michael Stiess", "Xander Houbaert", "Rolf Jaussi", "Daniel Frey", "Richard A. Kammerer", "Xavier Deupi", "Jean-Pierre de Villartay", "Andreas Lüthi", "Yann Humeau", "Jean Pieters"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1001820.g006", "stats"=>{"downloads"=>0, "page_views"=>22, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Stimulus_dependent_association_of_coronin_1_with_G_945_s_/974097", "title"=>"Stimulus-dependent association of coronin 1 with Gαs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-25 04:02:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1434950"], "description"=>"<p>(A) Coronin 1–expressing or control Mel JuSo cells were left untreated or stimulated with isoproterenol (5 µM, 4 min) and processed for cAMP analysis using ELISA (see <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001820#s4\" target=\"_blank\">Materials and Methods</a>). The values were normalized to total protein amounts (pmol cAMP/mg protein). The data shown are a representative of three independent experiments. Data represent mean and SEM. (B, C) FRET-based analysis of cAMP production upon stimulation of wild-type or coronin 1–expressing Mel JuSo cells that had been transfected with pICUE3 with isoproterenol (10 µM). (B) the normalized emission ratio (CFP/YFP) time course of coronin 1–expressing (gray) or wild type (red) Mel JuSo cells shown in C (mean, <i>n</i> = 50). (C) Stills from movies representing the normalized CFP/YFP emission ratios. Arrows indicate the time of isoproterenol addition. See also <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001820#pbio.1001820.s014\" target=\"_blank\">Movies S1</a> and <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001820#pbio.1001820.s015\" target=\"_blank\">S2</a>. (D–F) FRET-based analysis of cAMP production upon stimulation of Latrunculin B–treated (4 µM, 30 min) wild-type or coronin 1–expressing Mel JuSo cells (transfected with pICUE3) with isoproterenol (5 µM, at T = 25 s). (D) The normalized emission ratio (CFP/YFP) time course of coronin 1–expressing (grey) or wild-type (red) Mel JuSo cells (mean, <i>n</i> = 30). (E) Coronin 1 and F-actin staining in untreated and Latrunculin B–treated cells. (F) Stills from movies representing the normalized CFP/YFP emission ratios. Arrows indicate the time of isoproterenol addition. See also <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001820#pbio.1001820.s016\" target=\"_blank\">Movies S3</a> and <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001820#pbio.1001820.s017\" target=\"_blank\">S4</a>.</p>", "links"=>[], "tags"=>["cell biology", "Signal transduction", "cell signaling", "neuroscience", "Molecular neuroscience", "fret-based", "coronin", "cells"], "article_id"=>974094, "categories"=>["Biological Sciences"], "users"=>["Rajesh Jayachandran", "Xiaolong Liu", "Somdeb BoseDasgupta", "Philipp Müller", "Chun-Lei Zhang", "Despina Moshous", "Vera Studer", "Jacques Schneider", "Christel Genoud", "Catherine Fossoud", "Frédéric Gambino", "Malik Khelfaoui", "Christian Müller", "Deborah Bartholdi", "Helene Rossez", "Michael Stiess", "Xander Houbaert", "Rolf Jaussi", "Daniel Frey", "Richard A. Kammerer", "Xavier Deupi", "Jean-Pierre de Villartay", "Andreas Lüthi", "Yann Humeau", "Jean Pieters"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1001820.g005", "stats"=>{"downloads"=>1, "page_views"=>52, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ELISA_and_FRET_based_analysis_of_cAMP_production_in_coronin_1_8211_positive_and_coronin_1_8211_negative_cells_in_the_presence_and_absence_of_F_actin_/974094", "title"=>"ELISA and FRET-based analysis of cAMP production in coronin 1–positive and coronin 1–negative cells in the presence and absence of F-actin.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-25 04:02:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1434939"], "description"=>"<p>(A, B) Analysis of synapses in brain tissue from wild-type or coronin 1–deficient mice (<i>n</i> = 3) by serial block face scanning by electron microscopy. Scale bar, 2 microns. Data shown are mean values ± SD, <i>p</i><0.01. (C) Hippocampal neurons were labeled using anti-vGLUT1 and anti-vGAT antibodies, followed by Alex Fluor 488 or 568 labeled secondary antibodies, respectively. The average number of inhibitory and excitatory synapses per 10 µm, as well as the E/I synapse ratio from wild-type or coronin 1–deficient neurons were determined. Quantitation (right panel) was done from 60 neuron terminals from four mice per genotype. For vGLUT1, <i>p</i><0.01; for vGAT, <i>p</i>>0.05; and for vGLUT/vGAT ratio, <i>p</i><0.001, Student's <i>t</i> test. (D) Scheme of the experimental preparation (LA, lateral amygdala) and pairing protocols used to induce LTP. (E) Coronin 1–deficient animals (Cor1 −/−, red symbols) exhibited normal thalamo-LA LTP [<i>p</i><0.05 versus baseline; <i>p</i>>0.05 versus wild-type (WT, gray symbols), Student's <i>t</i> test]. Scale bars, 2 mV and 10 ms. (F) Cortico-LA LTP is completely absent in coronin 1–deficient mice (red symbols). Strong cortico-LA LTP was induced in wild-type [WT (grey symbols), <i>n</i> = 12, <i>p</i><0.05 versus baseline, <i>p</i><0.05 versus Cor 1 −/− (red symbols), Student's <i>t</i> test), but not in coronin 1–deficient mice [Cor 1 −/− (red), <i>n</i> = 7, <i>p</i>>0.05 versus baseline, Student's <i>t</i> test]. Scale bars, 50 pA and 20 ms. (G) Synaptic transmission and PPR in wild-type (left) and coronin 1–deficient mice (right) in the presence of forskolin. Forskolin enhances synaptic transmission and decreases PPR at cortico-LA synapses in WT (<i>n</i> = 12, <i>p</i><0.05 versus pre-forskolin baseline), but not in Cor 1 −/− (<i>n</i> = 7, <i>p</i>>0.05 versus pre-forskolin baseline, Student's <i>t</i> test) mice. Scale bars, 100 pA and 20 ms. See <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001820#pbio.1001820.s018\" target=\"_blank\">Table S1</a> for additional statistics.</p>", "links"=>[], "tags"=>["cell biology", "Signal transduction", "cell signaling", "neuroscience", "Molecular neuroscience", "synapse", "cortico-la", "synaptic", "plasticity", "coronin"], "article_id"=>974090, "categories"=>["Biological Sciences"], "users"=>["Rajesh Jayachandran", "Xiaolong Liu", "Somdeb BoseDasgupta", "Philipp Müller", "Chun-Lei Zhang", "Despina Moshous", "Vera Studer", "Jacques Schneider", "Christel Genoud", "Catherine Fossoud", "Frédéric Gambino", "Malik Khelfaoui", "Christian Müller", "Deborah Bartholdi", "Helene Rossez", "Michael Stiess", "Xander Houbaert", "Rolf Jaussi", "Daniel Frey", "Richard A. Kammerer", "Xavier Deupi", "Jean-Pierre de Villartay", "Andreas Lüthi", "Yann Humeau", "Jean Pieters"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1001820.g003", "stats"=>{"downloads"=>2, "page_views"=>35, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Decreased_E_I_synapse_ratio_and_loss_of_cortico_LA_synaptic_plasticity_in_the_absence_of_coronin_1_/974090", "title"=>"Decreased E/I synapse ratio and loss of cortico-LA synaptic plasticity in the absence of coronin 1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-25 04:02:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1434955"], "description"=>"<p>(A) Scheme of the in vivo preparation. (B) Cannula implantation loci in both animal cohorts: single injection mice (left) and double injection mice (right). (C) Contextual learning was tested before (Ctrl) and 12 h later following single (<i>n</i> = 10 WT and 11 coronin 1 −/−) and double cAMP injections (<i>n</i> = 10 for both genotypes). Single injections, <i>p</i><0.01. Double injections, <i>p</i>>0.05, RM-ANOVA, see also <a href=\"http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001820#pbio.1001820.s018\" target=\"_blank\">Table S1</a>.</p>", "links"=>[], "tags"=>["cell biology", "Signal transduction", "cell signaling", "neuroscience", "Molecular neuroscience", "conditioning", "coronin", "mice", "vivo", "8-br-camp", "infusion"], "article_id"=>974099, "categories"=>["Biological Sciences"], "users"=>["Rajesh Jayachandran", "Xiaolong Liu", "Somdeb BoseDasgupta", "Philipp Müller", "Chun-Lei Zhang", "Despina Moshous", "Vera Studer", "Jacques Schneider", "Christel Genoud", "Catherine Fossoud", "Frédéric Gambino", "Malik Khelfaoui", "Christian Müller", "Deborah Bartholdi", "Helene Rossez", "Michael Stiess", "Xander Houbaert", "Rolf Jaussi", "Daniel Frey", "Richard A. Kammerer", "Xavier Deupi", "Jean-Pierre de Villartay", "Andreas Lüthi", "Yann Humeau", "Jean Pieters"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1001820.g008", "stats"=>{"downloads"=>1, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Rescue_of_fear_conditioning_in_coronin_1_8211_deficient_mice_by_in_vivo_8_Br_cAMP_infusion_into_amygdala_/974099", "title"=>"Rescue of fear conditioning in coronin 1–deficient mice by in vivo 8-Br-cAMP infusion into amygdala.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-25 04:02:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1434954"], "description"=>"<p>(A, B) Cells were transfected with the different constructs indicated, stimulated with 10 µM isoproterenol (A, 4 min; B, 10 min) followed by lysis and analyzed for cAMP. Panel B shows the results of immunoprecipitating Gα followed by immunoblotting for coronin 1 (upper panels). The lower panels in B show the immunoblots for Gα and coronin 1 following SDS-PAGE of the transfected cell lysates. (C) Purified Gαs was covalently immobilized on an NTA sensor chip surface through EDC/NHS chemistry. The indicated concentrations of coronin 1 or coronin 1 mutant were sequentially injected into the SPR sensor chip. The kinetic data were collected and analyzed. The Relative Response Units (RUs) of stable binding under each different concentration is shown in the lower panel.</p>", "links"=>[], "tags"=>["cell biology", "Signal transduction", "cell signaling", "neuroscience", "Molecular neuroscience", "coronin", "cells", "vitro", "plasmon"], "article_id"=>974098, "categories"=>["Biological Sciences"], "users"=>["Rajesh Jayachandran", "Xiaolong Liu", "Somdeb BoseDasgupta", "Philipp Müller", "Chun-Lei Zhang", "Despina Moshous", "Vera Studer", "Jacques Schneider", "Christel Genoud", "Catherine Fossoud", "Frédéric Gambino", "Malik Khelfaoui", "Christian Müller", "Deborah Bartholdi", "Helene Rossez", "Michael Stiess", "Xander Houbaert", "Rolf Jaussi", "Daniel Frey", "Richard A. Kammerer", "Xavier Deupi", "Jean-Pierre de Villartay", "Andreas Lüthi", "Yann Humeau", "Jean Pieters"], "doi"=>"https://dx.doi.org/10.1371/journal.pbio.1001820.g007", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Analysis_of_coronin_1_8211_G_945_interaction_in_cells_and_in_vitro_by_surface_plasmon_resonance_/974098", "title"=>"Analysis of coronin 1–Gα interaction in cells and in vitro by surface plasmon resonance.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-25 04:02:31"}

PMC Usage Stats | Further Information

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  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"18", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
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  • {"unique-ip"=>"18", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
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  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"14", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"18", "full-text"=>"17", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
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  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"18", "cited-by"=>"1", "year"=>"2019", "month"=>"12"}
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  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"22", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"9"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"10"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[291]}, {"subject_area"=>"/Biology and life sciences/Behavior", "average_usage"=>[333]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[286]}, {"subject_area"=>"/Biology and life sciences/Neuroscience", "average_usage"=>[289]}, {"subject_area"=>"/Biology and life sciences/Physiology", "average_usage"=>[280]}, {"subject_area"=>"/Biology and life sciences/Zoology", "average_usage"=>[329]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[285]}, {"subject_area"=>"/Medicine and health sciences/Anatomy", "average_usage"=>[266]}, {"subject_area"=>"/Medicine and health sciences/Pharmacology", "average_usage"=>[286]}, {"subject_area"=>"/Medicine and health sciences/Physiology", "average_usage"=>[278]}]}
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Net::HTTPTooManyRequests

Source
Scopus
Time
2019-09-27 15:15:14 UTC
Target URL
https://api.elsevier.com/content/search/index:SCOPUS?query=DOI(10.1371%2Fjournal.pbio.1001820)
Trace

/app/models/concerns/networkable.rb:21:in `get_result'
/app/models/source.rb:165:in `get_data'
/app/models/retrieval_status.rb:47:in `perform_get_data'
/app/jobs/source_job.rb:52:in `block (2 levels) in perform'
/app/jobs/source_job.rb:51:in `block in perform'
/app/jobs/source_job.rb:35:in `each'
/app/jobs/source_job.rb:35:in `perform'