A flagellum-specific chaperone facilitates assembly of the core type III export apparatus of the bacterial flagellum
Publication Date
August 03, 2017
Journal
PLOS Biology
Authors
Florian D. Fabiani, Thibaud T. Renault, Britta Peters, Tobias Dietsche, et al
Volume
15
Issue
8
Pages
e2002267
DOI
https://dx.plos.org/10.1371/journal.pbio.2002267
Publisher URL
http://journals.plos.org/plosbiology/article?id=10.1371%2Fjournal.pbio.2002267
Scopus
85028559521
Mendeley
http://www.mendeley.com/research/flagellumspecific-chaperone-facilitates-assembly-core-type-iii-export-apparatus-bacterial-flagellum
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Mendeley | Further Information

{"title"=>"A flagellum-specific chaperone facilitates assembly of the core type III export apparatus of the bacterial flagellum", "type"=>"journal", "authors"=>[{"first_name"=>"Florian D.", "last_name"=>"Fabiani", "scopus_author_id"=>"56428775500"}, {"first_name"=>"Thibaud T.", "last_name"=>"Renault", "scopus_author_id"=>"47161542800"}, {"first_name"=>"Britta", "last_name"=>"Peters", "scopus_author_id"=>"57194450973"}, {"first_name"=>"Tobias", "last_name"=>"Dietsche", "scopus_author_id"=>"57192711896"}, {"first_name"=>"Eric J.C.", "last_name"=>"Gálvez", "scopus_author_id"=>"56898698800"}, {"first_name"=>"Alina", "last_name"=>"Guse", "scopus_author_id"=>"57195514227"}, {"first_name"=>"Karen", "last_name"=>"Freier", "scopus_author_id"=>"57195510053"}, {"first_name"=>"Emmanuelle", "last_name"=>"Charpentier", "scopus_author_id"=>"16169133500"}, {"first_name"=>"Till", "last_name"=>"Strowig", "scopus_author_id"=>"6506927062"}, {"first_name"=>"Mirita", "last_name"=>"Franz-Wachtel", "scopus_author_id"=>"26022391800"}, {"first_name"=>"Boris", "last_name"=>"Macek", "scopus_author_id"=>"15759963100"}, {"first_name"=>"Samuel", "last_name"=>"Wagner", "scopus_author_id"=>"16481669500"}, {"first_name"=>"Michael", "last_name"=>"Hensel", "scopus_author_id"=>"7004523452"}, {"first_name"=>"Marc", "last_name"=>"Erhardt", "scopus_author_id"=>"23667111900"}], "year"=>2017, "source"=>"PLoS Biology", "identifiers"=>{"doi"=>"10.1371/journal.pbio.2002267", "pui"=>"618047107", "scopus"=>"2-s2.0-85028559521", "issn"=>"15457885", "pmid"=>"28771474", "sgr"=>"85028559521", "isbn"=>"1111111111"}, "id"=>"c9c0acb5-0331-3b94-a5af-33b333f00f59", "abstract"=>"Many bacteria move using a complex, self-assembling nanomachine, the bacterial flagellum. Biosynthesis of the flagellum depends on a flagellar-specific type III secretion system (T3SS), a protein export machine homologous to the export machinery of the virulence-associated injectisome. Six cytoplasmic (FliH/I/J/G/M/N) and seven integral-membrane proteins (FlhA/B FliF/O/P/Q/R) form the flagellar basal body and are involved in the transport of flagellar building blocks across the inner membrane in a proton motive force-dependent manner. However, how the large, multi-component transmembrane export gate complex assembles in a coordinated manner remains enigmatic. Specific for most flagellar T3SSs is the presence of FliO, a small bitopic membrane protein with a large cytoplasmic domain. The function of FliO is unknown, but homologs of FliO are found in >80% of all flagellated bacteria. Here, we demonstrate that FliO protects FliP from proteolytic degradation and promotes the formation of a stable FliP–FliR complex required for the assembly of a functional core export apparatus. We further reveal the subcellular localization of FliO by super-resolution microscopy and show that FliO is not part of the assembled flagellar basal body. In summary, our results suggest that FliO functions as a novel, flagellar T3SS-specific chaperone, which facilitates quality control and productive assembly of the core T3SS export machinery.", "link"=>"http://www.mendeley.com/research/flagellumspecific-chaperone-facilitates-assembly-core-type-iii-export-apparatus-bacterial-flagellum", "reader_count"=>32, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Researcher"=>5, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>12, "Student > Master"=>4, "Other"=>2, "Student > Bachelor"=>2, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Researcher"=>5, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>12, "Student > Master"=>4, "Other"=>2, "Student > Bachelor"=>2, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>13, "Mathematics"=>1, "Agricultural and Biological Sciences"=>8, "Medicine and Dentistry"=>1, "Computer Science"=>1, "Immunology and Microbiology"=>6}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>6}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>8}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>13}, "Mathematics"=>{"Mathematics"=>1}, "Unspecified"=>{"Unspecified"=>2}}, "group_count"=>0}

Scopus | Further Information

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