Robustness of DNA Repair through Collective Rate Control
Publication Date
January 30, 2014
Journal
PLOS Computational Biology
Authors
Paul Verbruggen, Tim Heinemann, Erik Manders, Gesa Von Bornstaedt, et al
Volume
10
Issue
1
Pages
e1003438
DOI
https://dx.plos.org/10.1371/journal.pcbi.1003438
Publisher URL
http://journals.plos.org/ploscompbiol/article?id=10.1371%2Fjournal.pcbi.1003438
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/24499930
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907289
Europe PMC
http://europepmc.org/abstract/MED/24499930
Web of Science
000337948500038
Scopus
84893758846
Mendeley
http://www.mendeley.com/research/robustness-dna-repair-through-collective-rate-control
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Robustness of DNA Repair through Collective Rate Control", "type"=>"journal", "authors"=>[{"first_name"=>"Paul", "last_name"=>"Verbruggen", "scopus_author_id"=>"6506964358"}, {"first_name"=>"Tim", "last_name"=>"Heinemann", "scopus_author_id"=>"56031394800"}, {"first_name"=>"Erik", "last_name"=>"Manders", "scopus_author_id"=>"7005805578"}, {"first_name"=>"Gesa", "last_name"=>"von Bornstaedt", "scopus_author_id"=>"26532043900"}, {"first_name"=>"Roel", "last_name"=>"van Driel", "scopus_author_id"=>"55287685600"}, {"first_name"=>"Thomas", "last_name"=>"Höfer", "scopus_author_id"=>"7006417468"}], "year"=>2014, "source"=>"PLoS Computational Biology", "identifiers"=>{"sgr"=>"84893758846", "pui"=>"372348762", "isbn"=>"1553-7358 (Electronic)\r1553-734X (Linking)", "doi"=>"10.1371/journal.pcbi.1003438", "issn"=>"1553734X", "scopus"=>"2-s2.0-84893758846", "pmid"=>"24499930"}, "id"=>"79e98ea6-0cb0-3782-9532-62256cb204ba", "abstract"=>"Author SummaryThe nucleotide-excision repair pathway removes mutagen-inflicted DNA lesions from the genome. Repair proteins recognize DNA lesions and form multi-protein complexes that catalyze the excision of the lesion and the re-synthesis of the excised part. Imaging the dynamics of fluorescently labeled repair proteins in living human cells has revealed that all factors continuously and rapidly exchange at repair sites. We asked how this dynamic mode of protein-complex assembly shapes the repair process. Measuring repair DNA synthesis in intact cells, we obtained a surprisingly simple result. Over the entire process, the rate is proportional to the amount of DNA lesions, where the proportionality factor is a single ‘slow’ rate constant. Such kinetic behavior is often regarded as evidence for a rate-limiting step, but we show here that it is an emergent property of the dynamic interplay of many repair proteins. As a consequence, the rate of DNA repair is a systems property that is controlled collectively by the expression levels of all repair factors. Given that transcription in living cells has similar dynamic features – rapidly exchanging components of the transcription machinery and slow bursts of mRNA synthesis – collective rate control might be a general property of chromatin-associated molecular machines.", "link"=>"http://www.mendeley.com/research/robustness-dna-repair-through-collective-rate-control", "reader_count"=>24, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>3, "Researcher"=>6, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>8, "Student > Postgraduate"=>1, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>3, "Researcher"=>6, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>8, "Student > Postgraduate"=>1, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>4, "Agricultural and Biological Sciences"=>11, "Philosophy"=>1, "Physics and Astronomy"=>4, "Chemistry"=>1, "Computer Science"=>1}, "reader_count_by_subdiscipline"=>{"Chemistry"=>{"Chemistry"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>4}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>11}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}, "Unspecified"=>{"Unspecified"=>2}, "Philosophy"=>{"Philosophy"=>1}}, "reader_count_by_country"=>{"United States"=>2, "Italy"=>1, "Germany"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84893758846"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84893758846?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84893758846&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84893758846&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84893758846", "dc:identifier"=>"SCOPUS_ID:84893758846", "eid"=>"2-s2.0-84893758846", "dc:title"=>"Robustness of DNA Repair through Collective Rate Control", "dc:creator"=>"Verbruggen P.", "prism:publicationName"=>"PLoS Computational Biology", "prism:issn"=>"1553734X", "prism:eIssn"=>"15537358", "prism:volume"=>"10", "prism:issueIdentifier"=>"1", "prism:pageRange"=>nil, "prism:coverDate"=>"2014-01-01", "prism:coverDisplayDate"=>"January 2014", "prism:doi"=>"10.1371/journal.pcbi.1003438", "citedby-count"=>"10", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"German Cancer Research Center", "affiliation-city"=>"Heidelberg", "affiliation-country"=>"Germany"}, {"@_fa"=>"true", "affilname"=>"Swammerdam Institute for Life Sciences", "affiliation-city"=>"Amsterdam", "affiliation-country"=>"Netherlands"}], "pubmed-id"=>"24499930", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e1003438", "source-id"=>"4000151810", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fploscompbiol%2Farticle%3Fid%3D10.1371%252Fjournal.pcbi.1003438", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"31", "xml_views"=>"7", "html_views"=>"197"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"111", "xml_views"=>"7", "html_views"=>"1195"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"26", "xml_views"=>"2", "html_views"=>"351"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"28", "xml_views"=>"2", "html_views"=>"302"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"29", "xml_views"=>"1", "html_views"=>"345"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"190"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"153"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"3", "html_views"=>"123"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"92"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"72"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"90"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"75"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"97"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"70"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"77"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"77"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"90"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"97"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"93"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"95"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"14"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"3", "html_views"=>"7"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"2", "html_views"=>"4"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"3", "html_views"=>"10"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1368677"], "description"=>"<p>(<b>A</b>) Sequential (above) and random (below) assembly schemes for a complex of three components (A, B and C); x<sub>0</sub> denotes the empty assembly site (e.g., a DNA lesion), x<sub>A</sub> the assembly site with component A bound etc. The complete complex (x<sub>ABC</sub>) catalyses a reaction (e.g., lesion repair) with rate r. The repair process consists of several such assembly-reaction modules (cf. <a href=\"http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003438#pcbi-1003438-g001\" target=\"_blank\">Fig. 1</a>). (<b>B</b>) The mean reaction time <i>τ</i> given by <a href=\"http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003438#pcbi.1003438.e002\" target=\"_blank\">Eq. (2)</a> depends on the number of assembling protein components <i>N</i> and on the assembly mechanism. Parameters: apparent on-rates <i>k</i> equal to off-rates (<i>k</i> = <i>l</i> = 1 min<sup>−1</sup>), <i>ρ</i> = 10 s<sup>−1</sup> (<b>C</b>) In the case of reversible assembly (<i>k</i> = <i>l</i> = 1 min<sup>−1</sup>, <i>N</i> = 9, random mechanism), the formation of the reaction product follows exponential kinetics (solid red line, fitted perfectly by a mono-exponential time course with time constant <i>τ</i>). Irreversible assembly (<i>l</i> = 0) follows a sigmoidal time course (dashed red line, <i>N</i> = 9, random mechanism, on-rate <i>k</i> = 0.037 min<sup>−1</sup> chosen to get the same time constant). The results for a sequential assembly mechanisms are qualitatively similar.</p>", "links"=>[], "tags"=>["Computational biology", "Biochemical simulations", "Molecular cell biology", "Nucleic acids", "dna", "DNA repair", "systems biology", "catalytic", "multi-protein"], "article_id"=>919756, "categories"=>["Biological Sciences"], "users"=>["Paul Verbruggen", "Tim Heinemann", "Erik Manders", "Gesa von Bornstaedt", "Roel van Driel", "Thomas Höfer"], "doi"=>"https://dx.doi.org/10.1371/journal.pcbi.1003438.g003", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Analytical_model_of_the_formation_of_a_catalytic_multi_protein_complex_on_DNA_/919756", "title"=>"Analytical model of the formation of a catalytic multi-protein complex on DNA.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-30 04:15:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1368673"], "description"=>"<p>(<b>A</b>) Principle of the sequential organization of chromatin-associated processes through cycles of protein recruitment, enzymatic modification and subsequent reorganization of the protein machinery. (<b>B</b>) Nucleotide excision repair in mammalian cells. Shown are the sequence of repair steps and, for each step, the proteins that need to assemble on the DNA to catalyse it.</p>", "links"=>[], "tags"=>["Computational biology", "Biochemical simulations", "Molecular cell biology", "Nucleic acids", "dna", "DNA repair", "systems biology"], "article_id"=>919752, "categories"=>["Biological Sciences"], "users"=>["Paul Verbruggen", "Tim Heinemann", "Erik Manders", "Gesa von Bornstaedt", "Roel van Driel", "Thomas Höfer"], "doi"=>"https://dx.doi.org/10.1371/journal.pcbi.1003438.g001", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Chromatin_associated_processes_/919752", "title"=>"Chromatin-associated processes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-30 04:15:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1368685"], "description"=>"<p>(<b>A</b>) XP–C XPC-eGFP cells were locally irradiated and fixed immediately. The distribution of inflicted 6-4PP lesions was determined by immunostaining followed by scoring of the immunofluorescence signal in <i>n</i> = 250 cells from five experiments (<b>B</b>) XP–C XPC-eGFP cells were locally irradiated and cultivated for 4 hours in the presence of EdU before fixation. Distribution of EdU incorporation was plotted based on measurements derived from <i>n</i> = 198 scored local damages derived from three independent experiments.</p>", "links"=>[], "tags"=>["Computational biology", "Biochemical simulations", "Molecular cell biology", "Nucleic acids", "dna", "DNA repair", "systems biology", "inflicted", "lesions", "edu", "incorporation"], "article_id"=>919764, "categories"=>["Biological Sciences"], "users"=>["Paul Verbruggen", "Tim Heinemann", "Erik Manders", "Gesa von Bornstaedt", "Roel van Driel", "Thomas Höfer"], "doi"=>"https://dx.doi.org/10.1371/journal.pcbi.1003438.g008", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Initial_distribution_of_inflicted_lesions_6_4_photoproducts_and_final_distribution_of_EdU_incorporation_match_/919764", "title"=>"Initial distribution of inflicted lesions (6-4 photoproducts) and final distribution of EdU incorporation match.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-30 04:15:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1368682"], "description"=>"<p>(<b>A</b>) Frequency histograms of endogenous XPC (<i>n</i> = 1727) and XPA (n = 1462 cells) concentration in HeLa cells. (<b>B</b>) Scatter plot of XPC-eGFP fluorescence in XP–C XPC-eGFP cells against an antibody recognizing XPC (left, <i>n</i> = 332 cells) and eGFP-XPA fluorescence in XP–A eGFP-XPA cell line against an antibody recognizing XPA (right, n = 2142 cells) as determined by quantitative (immuno) fluorescence microscopy. (<b>C</b>) Scatter plot of endogenous XPC nuclear concentration versus endogenous XPC concentration in DNA damaged areas 30 minutes post-irradiation. (<b>D</b>) Endogenous XPA nuclear concentration versus endogenous XPA concentration in damaged areas 60 minutes post irradiation. (<b>E</b>) Scatter plot of endogenous XPC nuclear concentration versus repair DNA synthesis as measured by EdU incorporation on local damage 30 minutes post-irradiation (<i>n</i> = 303 in three independent experiments). (<b>F</b>) Scatter plot as in (<b>E</b>), but for XPA (n = 170 cells). Cells were analysed 60 minutes after inflicting UV damage, i.e. at maximum XPA accumulation. 95% confidence bounds of all correlation coeffiecients <i>r</i> were estimated by non-parametric bootstrap and are given in brackets.</p>", "links"=>[], "tags"=>["Computational biology", "Biochemical simulations", "Molecular cell biology", "Nucleic acids", "dna", "DNA repair", "systems biology", "variability", "xpc", "xpa"], "article_id"=>919761, "categories"=>["Biological Sciences"], "users"=>["Paul Verbruggen", "Tim Heinemann", "Erik Manders", "Gesa von Bornstaedt", "Roel van Driel", "Thomas Höfer"], "doi"=>"https://dx.doi.org/10.1371/journal.pcbi.1003438.g006", "stats"=>{"downloads"=>0, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Natural_expression_variability_of_the_repair_factors_XPC_and_XPA_has_small_effect_on_DNA_repair_synthesis_/919761", "title"=>"Natural expression variability of the repair factors XPC and XPA has small effect on DNA repair synthesis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-30 04:15:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1368681"], "description"=>"<p>(<b>A</b>) Response coefficients for the control of the repair rate by the concentrations of the repair factors. (<b>B</b>) Repair rate as a function of concentration changes in individual repair factors.</p>", "links"=>[], "tags"=>["Computational biology", "Biochemical simulations", "Molecular cell biology", "Nucleic acids", "dna", "DNA repair", "systems biology", "distributed"], "article_id"=>919760, "categories"=>["Biological Sciences"], "users"=>["Paul Verbruggen", "Tim Heinemann", "Erik Manders", "Gesa von Bornstaedt", "Roel van Driel", "Thomas Höfer"], "doi"=>"https://dx.doi.org/10.1371/journal.pcbi.1003438.g005", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Control_over_the_rate_of_DNA_repair_predicted_to_be_small_and_distributed_over_all_repair_proteins_/919760", "title"=>"Control over the rate of DNA repair predicted to be small and distributed over all repair proteins.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-30 04:15:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1368695", "https://ndownloader.figshare.com/files/1368696", "https://ndownloader.figshare.com/files/1368697", "https://ndownloader.figshare.com/files/1368698", "https://ndownloader.figshare.com/files/1368699", "https://ndownloader.figshare.com/files/1368700"], "description"=>"<div><p>DNA repair and other chromatin-associated processes are carried out by enzymatic macromolecular complexes that assemble at specific sites on the chromatin fiber. How the rate of these molecular machineries is regulated by their constituent parts is poorly understood. Here we quantify nucleotide-excision DNA repair in mammalian cells and find that, despite the pathways' molecular complexity, repair effectively obeys slow first-order kinetics. Theoretical analysis and data-based modeling indicate that these kinetics are not due to a singular rate-limiting step. Rather, first-order kinetics emerge from the interplay of rapidly and reversibly assembling repair proteins, stochastically distributing DNA lesion repair over a broad time period. Based on this mechanism, the model predicts that the repair proteins collectively control the repair rate. Exploiting natural cell-to-cell variability, we corroborate this prediction for the lesion-recognition factor XPC and the downstream factor XPA. Our findings provide a rationale for the emergence of slow time scales in chromatin-associated processes from fast molecular steps and suggest that collective rate control might be a widespread mode of robust regulation in DNA repair and transcription.</p></div>", "links"=>[], "tags"=>["Computational biology", "Biochemical simulations", "Molecular cell biology", "Nucleic acids", "dna", "DNA repair", "systems biology"], "article_id"=>919773, "categories"=>["Biological Sciences"], "users"=>["Paul Verbruggen", "Tim Heinemann", "Erik Manders", "Gesa von Bornstaedt", "Roel van Driel", "Thomas Höfer"], "doi"=>["https://dx.doi.org/10.1371/journal.pcbi.1003438.s001", "https://dx.doi.org/10.1371/journal.pcbi.1003438.s002", "https://dx.doi.org/10.1371/journal.pcbi.1003438.s003", "https://dx.doi.org/10.1371/journal.pcbi.1003438.s004", "https://dx.doi.org/10.1371/journal.pcbi.1003438.s005", "https://dx.doi.org/10.1371/journal.pcbi.1003438.s006"], "stats"=>{"downloads"=>15, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Robustness_of_DNA_Repair_through_Collective_Rate_Control_/919773", "title"=>"Robustness of DNA Repair through Collective Rate Control", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-01-30 04:15:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1368679"], "description"=>"<p>(<b>A</b>) The model of NER distinguishes 5 DNA repair intermediates as indicated (I–V) that are interconverted by enzymatic steps: <i>α</i>, DNA unwinding; <i>β</i>, dual incision; <i>γ</i>, re-synthesis; <i>δ</i>, re-chromatinization; <i>ε</i>, re-annealing of unwound DNA. The repair proteins bind reversibly to the respective repair intermediates as indicated. Underlined proteins together catalyse the transition from one repair intermediate to the next. (<b>B</b>) Best-fit values for the model parameters with 95% confidence intervals determined by the profile-likelihood method <a href=\"http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003438#pcbi.1003438-Raue1\" target=\"_blank\">[25]</a>. The binding rate constants are shown as apparent first-order rate constants obtained by multiplying the fitted <i>k</i><sub>on</sub> values with the free concentrations of the respective repair factors (taken from <a href=\"http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003438#pcbi.1003438-Luijsterburg1\" target=\"_blank\">[8]</a>). (<b>C</b>) Comparison of experimental and simulated time courses for XPC and EdU incorporation. (<b>D</b>) Simulated accumulation time courses (lines) of all NER factors in the model compared to experimental data (dots) <a href=\"http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003438#pcbi.1003438-Luijsterburg1\" target=\"_blank\">[8]</a>. (<b>E</b>) Simulated FLIP (fluorescence loss in photobleaching) curves (lines) of all NER factors in the model compared to experimental data (dots) <a href=\"http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003438#pcbi.1003438-Luijsterburg1\" target=\"_blank\">[8]</a>. These curves show the short dwell times resulting from individual binding events. (<b>F</b>) Computed (lines) and measured (dots with error bars) time courses of the repair intermediates. The simulated time course of the relative amount of the incised DNA intermediate is shown.</p>", "links"=>[], "tags"=>["Computational biology", "Biochemical simulations", "Molecular cell biology", "Nucleic acids", "dna", "DNA repair", "systems biology", "ner", "reproduces", "kinetics", "proteins"], "article_id"=>919758, "categories"=>["Biological Sciences"], "users"=>["Paul Verbruggen", "Tim Heinemann", "Erik Manders", "Gesa von Bornstaedt", "Roel van Driel", "Thomas Höfer"], "doi"=>"https://dx.doi.org/10.1371/journal.pcbi.1003438.g004", "stats"=>{"downloads"=>2, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Data_based_model_of_NER_reproduces_in_vivo_kinetics_of_the_repair_proteins_and_repair_reactions_/919758", "title"=>"Data-based model of NER reproduces <i>in vivo</i> kinetics of the repair proteins and repair reactions.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-30 04:15:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1368675"], "description"=>"<p>(<b>A</b>) DNA in HeLa cells (upper panel) or XP–C XPC-eGFP cells (lower panel) was locally damaged by UV irradiation and subsequently incubated for 30 minutes in the presence of 10 µM EdU. Arrows indicate local damage areas as revealed by accumulation of endogenous XPC (upper panel) or stably expressed XPC-eGFP (lower panel); EdU incorporation is a measure for repair DNA synthesis. (<b>B</b>) Repair DNA synthesis on sites of local damage as determined by quantitative microscopy coincides with the removal of 6-4PPs measured previously in the same set-up by antibody staining <a href=\"http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003438#pcbi.1003438-Luijsterburg1\" target=\"_blank\">[8]</a>. Graphs display the means ± SD, <i>n</i> = 50–70 locally damaged cells per time point for damaged DNA and <i>n</i> = 150 locally damaged cells (derived from three independent experiments) per time point for repaired DNA. (<b>C</b>) Comparison of increase in EdU incorporation in locally damaged DNA regions with increase in PCNA accumulation as measured previously <a href=\"http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003438#pcbi.1003438-Luijsterburg1\" target=\"_blank\">[8]</a>. (<b>D</b>) Plotting the EdU data according to a linearization of <a href=\"http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003438#pcbi.1003438.e001\" target=\"_blank\">Eq. (1)</a> reveals a single-exponential time course.</p>", "links"=>[], "tags"=>["Computational biology", "Biochemical simulations", "Molecular cell biology", "Nucleic acids", "dna", "DNA repair", "systems biology", "incorporation", "colocalizes", "sites", "reflects", "synthesis"], "article_id"=>919754, "categories"=>["Biological Sciences"], "users"=>["Paul Verbruggen", "Tim Heinemann", "Erik Manders", "Gesa von Bornstaedt", "Roel van Driel", "Thomas Höfer"], "doi"=>"https://dx.doi.org/10.1371/journal.pcbi.1003438.g002", "stats"=>{"downloads"=>1, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_EdU_incorporation_colocalizes_with_sites_of_local_damage_and_reflects_repair_DNA_synthesis_quantitatively_/919754", "title"=>"EdU incorporation colocalizes with sites of local damage and reflects repair DNA synthesis quantitatively.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-30 04:15:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1368689"], "description"=>"<p>NA, not applicable. K<sub>D</sub> (k<sub>off</sub>/k<sub>on</sub>) values are given for every repair protein and arranged in columns. Reference parameter set and 95% confidence intervals (in parentheses) are shown.</p>", "links"=>[], "tags"=>["Computational biology", "Biochemical simulations", "Molecular cell biology", "Nucleic acids", "dna", "DNA repair", "systems biology"], "article_id"=>919768, "categories"=>["Biological Sciences"], "users"=>["Paul Verbruggen", "Tim Heinemann", "Erik Manders", "Gesa von Bornstaedt", "Roel van Driel", "Thomas Höfer"], "doi"=>"https://dx.doi.org/10.1371/journal.pcbi.1003438.t001", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_K_D_values_/919768", "title"=>"K<sub>D</sub> values.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-01-30 04:15:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1368687"], "description"=>"<p>Comparison of modeled and measured correlation of nuclear XPC concentration and XPC accumulation in the locally damaged region (<b>A</b>) and between nuclear XPC concentration and DNA synthesis (<b>B</b>). Red lines represent linear regression with correlation coefficient <i>r</i> and <i>p</i>-value. (<b>C</b>) and (<b>D</b>) as in (A) and (B) but for XPA. (<b>E</b>) Simulated variability of the DNA repair kinetic under the influence of a single variable protein (dots) and of all variable components (triangles). For comparison the experimental <i>CV</i> is shown at five time points and error bars obtained with nonparametric bootstrap. (<b>F</b>) Time evolution of distribution of EdU incorporation: measured (red bars) versus simulated (blue lines). 95% confidence bounds of all correlation coeffiecients <i>r</i> were estimated by non-parametric bootstrap and are given in brackets.</p>", "links"=>[], "tags"=>["Computational biology", "Biochemical simulations", "Molecular cell biology", "Nucleic acids", "dna", "DNA repair", "systems biology", "ner", "inflicted", "lesions", "quantitatively"], "article_id"=>919766, "categories"=>["Biological Sciences"], "users"=>["Paul Verbruggen", "Tim Heinemann", "Erik Manders", "Gesa von Bornstaedt", "Roel van Driel", "Thomas Höfer"], "doi"=>"https://dx.doi.org/10.1371/journal.pcbi.1003438.g009", "stats"=>{"downloads"=>0, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Variable_expression_levels_of_NER_factors_and_inflicted_DNA_lesions_quantitatively_account_for_the_distribution_of_repair_rates_/919766", "title"=>"Variable expression levels of NER factors and inflicted DNA lesions quantitatively account for the distribution of repair rates.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-30 04:15:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1368683"], "description"=>"<p>(A) Variability of XPC-eGFP (left, n = 332) concentration in stably transfected XP–C. (B) Distribution of incorporated EdU in local damages measured in XP–C XPC-eGFP (red) or HeLa (blue). n = 154 cells per time point per cell line.</p>", "links"=>[], "tags"=>["Computational biology", "Biochemical simulations", "Molecular cell biology", "Nucleic acids", "dna", "DNA repair", "systems biology", "ner", "xpc-egfp", "complemented"], "article_id"=>919762, "categories"=>["Biological Sciences"], "users"=>["Paul Verbruggen", "Tim Heinemann", "Erik Manders", "Gesa von Bornstaedt", "Roel van Driel", "Thomas Höfer"], "doi"=>"https://dx.doi.org/10.1371/journal.pcbi.1003438.g007", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Variability_of_NER_in_XPC_eGFP_complemented_XP_8211_C_patient_cell_line_is_comparable_to_native_NER_/919762", "title"=>"Variability of NER in XPC-eGFP complemented XP–C patient cell line is comparable to native NER.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-30 04:15:13"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"23", "full-text"=>"16", "pdf"=>"12", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"15", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"8", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"38", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"8", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"0", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"2", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[291]}, {"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[282]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[286]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[306, 482]}, {"subject_area"=>"/Physical sciences", "average_usage"=>[271]}, {"subject_area"=>"/Physical sciences/Physics", "average_usage"=>[266]}]}
Loading … Spinner
There are currently no alerts