Amplification of a Cytochrome P450 Gene Is Associated with Resistance to Neonicotinoid Insecticides in the Aphid Myzus persicae
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{"title"=>"Amplification of a cytochrome P450 gene is associated with resistance to neonicotinoid insecticides in the aphid Myzus persicae", "type"=>"journal", "authors"=>[{"first_name"=>"Alin M.", "last_name"=>"Puinean", "scopus_author_id"=>"36965120500"}, {"first_name"=>"Stephen P.", "last_name"=>"Foster", "scopus_author_id"=>"35760293400"}, {"first_name"=>"Linda", "last_name"=>"Oliphant", "scopus_author_id"=>"25822516600"}, {"first_name"=>"Ian", "last_name"=>"Denholm", "scopus_author_id"=>"7003308150"}, {"first_name"=>"Linda M.", "last_name"=>"Field", "scopus_author_id"=>"7203030239"}, {"first_name"=>"Neil S.", "last_name"=>"Millar", "scopus_author_id"=>"7006347978"}, {"first_name"=>"Martin S.", "last_name"=>"Williamson", "scopus_author_id"=>"55664861400"}, {"first_name"=>"Chris", "last_name"=>"Bass", "scopus_author_id"=>"7005141118"}], "year"=>2010, "source"=>"PLoS Genetics", "identifiers"=>{"sgr"=>"77954154230", "doi"=>"10.1371/journal.pgen.1000999", "pui"=>"359100515", "pmid"=>"20585623", "scopus"=>"2-s2.0-77954154230", "issn"=>"15537390", "isbn"=>"15537404", "arxiv"=>"16953491"}, "id"=>"420b5ea4-f68a-3764-88b3-2a526308af01", "abstract"=>"The aphid Myzus persicae is a globally significant crop pest that has evolved high levels of resistance to almost all classes of insecticide. To date, the neonicotinoids, an economically important class of insecticides that target nicotinic acetylcholine receptors (nAChRs), have remained an effective control measure; however, recent reports of resistance in M. persicae represent a threat to the long-term efficacy of this chemical class. In this study, the mechanisms underlying resistance to the neonicotinoid insecticides were investigated using biological, biochemical, and genomic approaches. Bioassays on a resistant M. persicae clone (5191A) suggested that P450-mediated detoxification plays a primary role in resistance, although additional mechanism(s) may also contribute. Microarray analysis, using an array populated with probes corresponding to all known detoxification genes in M. persicae, revealed constitutive over-expression (22-fold) of a single P450 gene (CYP6CY3); and quantitative PCR showed that the over-expression is due, at least in part, to gene amplification. This is the first report of a P450 gene amplification event associated with insecticide resistance in an agriculturally important insect pest. The microarray analysis also showed over-expression of several gene sequences that encode cuticular proteins (2-16-fold), and artificial feeding assays and in vivo penetration assays using radiolabeled insecticide provided direct evidence of a role for reduced cuticular penetration in neonicotinoid resistance. Conversely, receptor radioligand binding studies and nucleotide sequencing of nAChR subunit genes suggest that target-site changes are unlikely to contribute to resistance to neonicotinoid insecticides in M. persicae.", "link"=>"http://www.mendeley.com/research/amplification-cytochrome-p450-gene-associated-resistance-neonicotinoid-insecticides-aphid-myzus-pers-5", "reader_count"=>128, "reader_count_by_academic_status"=>{"Unspecified"=>6, "Professor > Associate Professor"=>9, "Researcher"=>24, "Student > Doctoral Student"=>7, "Student > Ph. D. Student"=>34, "Student > Postgraduate"=>3, "Student > Master"=>19, "Other"=>2, "Student > Bachelor"=>17, "Lecturer"=>2, "Lecturer > Senior Lecturer"=>1, "Professor"=>4}, "reader_count_by_user_role"=>{"Unspecified"=>6, "Professor > Associate Professor"=>9, "Researcher"=>24, "Student > Doctoral Student"=>7, "Student > Ph. D. Student"=>34, "Student > Postgraduate"=>3, "Student > Master"=>19, "Other"=>2, "Student > Bachelor"=>17, "Lecturer"=>2, "Lecturer > Senior Lecturer"=>1, "Professor"=>4}, "reader_count_by_subject_area"=>{"Unspecified"=>9, "Environmental Science"=>3, "Biochemistry, Genetics and Molecular Biology"=>11, "Nursing and Health Professions"=>1, "Agricultural and Biological Sciences"=>98, "Medicine and Dentistry"=>2, "Physics and Astronomy"=>1, "Chemistry"=>3}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Chemistry"=>{"Chemistry"=>3}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>98}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>11}, "Unspecified"=>{"Unspecified"=>9}, "Environmental Science"=>{"Environmental Science"=>3}}, "reader_count_by_country"=>{"Greece"=>1, "Canada"=>2, "United States"=>3, "Japan"=>1, "China"=>1, "Brazil"=>1, "United Kingdom"=>2, "France"=>1, "Chile"=>1, "Portugal"=>1, "Switzerland"=>1, "Spain"=>1}, "group_count"=>4}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/843112"], "description"=>"<p>5′ and 3′ untranslated regions are highlighted in grey. Sites of amino acid substitutions are boxed. Conserved domains common to cytochrome P450s or to CYP6 members are highlighted in blue such as the oxygen binding motif (position 316), the heme binding motif (position 447), the helix K motif (position 374) and a sequence motif (position 429) specific to CYP6 members.</p>", "links"=>[], "tags"=>["mrna", "persicae"], "article_id"=>513568, "categories"=>["Molecular Biology", "Genetics"], "users"=>["Alin M. Puinean", "Stephen P. Foster", "Linda Oliphant", "Ian Denholm", "Linda M. Field", "Neil S. Millar", "Martin S. Williamson", "Chris Bass"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1000999.g001", "stats"=>{"downloads"=>3, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Complete_mRNA_sequence_of_M_persicae_CYP6CY3_/513568", "title"=>"Complete mRNA sequence of <i>M. persicae CYP6CY3.</i>", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-06-24 00:59:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/843551"], "description"=>"<p>Selected genes identified by microarray as significantly differentially transcribed between the insecticide resistant <i>M. persicae</i> clone 5191 and the susceptible strain 4106a.</p>", "links"=>[], "tags"=>["genes", "microarray", "differentially", "transcribed", "insecticide", "resistant", "clone", "5191", "susceptible"], "article_id"=>513995, "categories"=>["Molecular Biology", "Genetics"], "users"=>["Alin M. Puinean", "Stephen P. Foster", "Linda Oliphant", "Ian Denholm", "Linda M. Field", "Neil S. Millar", "Martin S. Williamson", "Chris Bass"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1000999.t002", "stats"=>{"downloads"=>3, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Selected_genes_identified_by_microarray_as_significantly_differentially_transcribed_between_the_insecticide_resistant_M_persicae_clone_5191_and_the_susceptible_strain_4106a_/513995", "title"=>"Selected genes identified by microarray as significantly differentially transcribed between the insecticide resistant <i>M. persicae</i> clone 5191 and the susceptible strain 4106a.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2010-06-24 01:06:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/843451"], "description"=>"<p>Results of artificial feeding bioassays with a range of neonicotinoids to the <i>M. persicae</i> clones 4106A (susceptible) and 5191A (resistant).</p>", "links"=>[], "tags"=>["feeding", "bioassays", "neonicotinoids", "clones", "4106a", "5191a"], "article_id"=>513901, "categories"=>["Molecular Biology", "Genetics"], "users"=>["Alin M. Puinean", "Stephen P. Foster", "Linda Oliphant", "Ian Denholm", "Linda M. Field", "Neil S. Millar", "Martin S. Williamson", "Chris Bass"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1000999.t004", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Results_of_artificial_feeding_bioassays_with_a_range_of_neonicotinoids_to_the_M_persicae_clones_4106A_susceptible_and_5191A_resistant_/513901", "title"=>"Results of artificial feeding bioassays with a range of neonicotinoids to the <i>M. persicae</i> clones 4106A (susceptible) and 5191A (resistant).", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2010-06-24 01:05:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/843357"], "description"=>"<p>Bars represent 95% confidence limits.</p>", "links"=>[], "tags"=>["imidacloprid", "binding", "membranes", "extracted"], "article_id"=>513810, "categories"=>["Molecular Biology", "Genetics"], "users"=>["Alin M. Puinean", "Stephen P. Foster", "Linda Oliphant", "Ian Denholm", "Linda M. Field", "Neil S. Millar", "Martin S. Williamson", "Chris Bass"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1000999.g004", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Specific_3_H_imidacloprid_binding_to_membranes_extracted_from_a_range_of_M_persicae_clones_/513810", "title"=>"Specific [<sup>3</sup>H] imidacloprid binding to membranes extracted from a range of <i>M. persicae</i> clones.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-06-24 01:03:30"}
  • {"files"=>["https://ndownloader.figshare.com/files/419863", "https://ndownloader.figshare.com/files/419877", "https://ndownloader.figshare.com/files/419893"], "description"=>"<div><p>The aphid <em>Myzus persicae</em> is a globally significant crop pest that has evolved high levels of resistance to almost all classes of insecticide. To date, the neonicotinoids, an economically important class of insecticides that target nicotinic acetylcholine receptors (nAChRs), have remained an effective control measure; however, recent reports of resistance in <em>M. persicae</em> represent a threat to the long-term efficacy of this chemical class. In this study, the mechanisms underlying resistance to the neonicotinoid insecticides were investigated using biological, biochemical, and genomic approaches. Bioassays on a resistant <em>M. persicae</em> clone (5191A) suggested that P450-mediated detoxification plays a primary role in resistance, although additional mechanism(s) may also contribute. Microarray analysis, using an array populated with probes corresponding to all known detoxification genes in <em>M. persicae</em>, revealed constitutive over-expression (22-fold) of a single P450 gene (<em>CYP6CY3</em>); and quantitative PCR showed that the over-expression is due, at least in part, to gene amplification. This is the first report of a P450 gene amplification event associated with insecticide resistance in an agriculturally important insect pest. The microarray analysis also showed over-expression of several gene sequences that encode cuticular proteins (2–16-fold), and artificial feeding assays and <em>in vivo</em> penetration assays using radiolabeled insecticide provided direct evidence of a role for reduced cuticular penetration in neonicotinoid resistance. Conversely, receptor radioligand binding studies and nucleotide sequencing of nAChR subunit genes suggest that target-site changes are unlikely to contribute to resistance to neonicotinoid insecticides in <em>M. persicae.</em></p></div>", "links"=>[], "tags"=>["amplification", "cytochrome", "p450", "neonicotinoid", "insecticides", "aphid"], "article_id"=>142900, "categories"=>["Molecular Biology", "Genetics"], "users"=>["Alin M. Puinean", "Stephen P. Foster", "Linda Oliphant", "Ian Denholm", "Linda M. Field", "Neil S. Millar", "Martin S. Williamson", "Chris Bass"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1000999.s001", "https://dx.doi.org/10.1371/journal.pgen.1000999.s002", "https://dx.doi.org/10.1371/journal.pgen.1000999.s003"], "stats"=>{"downloads"=>7, "page_views"=>23, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Amplification_of_a_Cytochrome_P450_Gene_Is_Associated_with_Resistance_to_Neonicotinoid_Insecticides_in_the_Aphid_Myzus_persicae_/142900", "title"=>"Amplification of a Cytochrome P450 Gene Is Associated with Resistance to Neonicotinoid Insecticides in the Aphid <em>Myzus persicae</em>", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2010-06-24 00:48:20"}
  • {"files"=>["https://ndownloader.figshare.com/files/843597"], "description"=>"<p>For comparison the fold change measured by microarray is also shown.</p>", "links"=>[], "tags"=>["cuticular", "proteins", "insecticide", "resistant", "clone", "5191", "susceptible", "quantitative"], "article_id"=>514037, "categories"=>["Molecular Biology", "Genetics"], "users"=>["Alin M. Puinean", "Stephen P. Foster", "Linda Oliphant", "Ian Denholm", "Linda M. Field", "Neil S. Millar", "Martin S. Williamson", "Chris Bass"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1000999.t003", "stats"=>{"downloads"=>5, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Fold_change_in_expression_of_CYP6CY3_and_cuticular_proteins_in_the_insecticide_resistant_M_persicae_clone_5191_compared_to_the_susceptible_reference_clone_4106A_as_determined_by_quantitative_PCR_/514037", "title"=>"Fold change in expression of <i>CYP6CY3</i> and cuticular proteins in the insecticide resistant <i>M. persicae</i> clone 5191 (compared to the susceptible reference clone 4106A) as determined by quantitative PCR.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2010-06-24 01:07:17"}
  • {"files"=>["https://ndownloader.figshare.com/files/843203"], "description"=>"<p>The start codon is boxed. An indel which introduces a stop codon in copy variant 4 is indicated by an arrow.</p>", "links"=>[], "tags"=>["n-terminal", "coding", "variants", "clone"], "article_id"=>513657, "categories"=>["Molecular Biology", "Genetics"], "users"=>["Alin M. Puinean", "Stephen P. Foster", "Linda Oliphant", "Ian Denholm", "Linda M. Field", "Neil S. Millar", "Martin S. Williamson", "Chris Bass"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1000999.g002", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Alignment_of_the_5_UTR_and_N_terminal_coding_sequence_of_CYP6CY3_copy_variants_in_the_M_persicae_clone_5191a_/513657", "title"=>"Alignment of the 5′UTR and N-terminal coding sequence of <i>CYP6CY3</i> copy variants in the <i>M. persicae</i> clone 5191a.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-06-24 01:00:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/843272"], "description"=>"<p>Graph shows the percentage of the initial [<sup>3</sup>H] imidacloprid dose recovered from the cuticle wash at different time points after application.</p>", "links"=>[], "tags"=>["penetration", "assay", "imidacloprid", "4106a", "5191a"], "article_id"=>513723, "categories"=>["Molecular Biology", "Genetics"], "users"=>["Alin M. Puinean", "Stephen P. Foster", "Linda Oliphant", "Ian Denholm", "Linda M. Field", "Neil S. Millar", "Martin S. Williamson", "Chris Bass"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1000999.g003", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Result_of_in_vivo_penetration_assay_using_3_H_imidacloprid_on_the_4106A_susceptible_and_5191A_resistant_clones_/513723", "title"=>"Result of <i>in vivo</i> penetration assay using [<sup>3</sup>H] imidacloprid on the 4106A (susceptible) and 5191A (resistant) clones.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-06-24 01:02:03"}
  • {"files"=>["https://ndownloader.figshare.com/files/843507"], "description"=>"<p>Results of full dose-response bioassays with a range of neonicotinoids to the <i>M. persicae</i> clones 4106A (susceptible) and 5191A (resistant) both with and without pre-treatment with the P450 inhibitor PBO.</p>", "links"=>[], "tags"=>["dose-response", "bioassays", "neonicotinoids", "clones", "4106a", "5191a", "pre-treatment", "p450", "inhibitor"], "article_id"=>513951, "categories"=>["Molecular Biology", "Genetics"], "users"=>["Alin M. Puinean", "Stephen P. Foster", "Linda Oliphant", "Ian Denholm", "Linda M. Field", "Neil S. Millar", "Martin S. Williamson", "Chris Bass"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1000999.t001", "stats"=>{"downloads"=>2, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Results_of_full_dose_response_bioassays_with_a_range_of_neonicotinoids_to_the_M_persicae_clones_4106A_susceptible_and_5191A_resistant_both_with_and_without_pre_treatment_with_the_P450_inhibitor_PBO_/513951", "title"=>"Results of full dose-response bioassays with a range of neonicotinoids to the <i>M. persicae</i> clones 4106A (susceptible) and 5191A (resistant) both with and without pre-treatment with the P450 inhibitor PBO.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2010-06-24 01:05:51"}

PMC Usage Stats | Further Information

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Relative Metric

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