Meiosis-Specific Loading of the Centromere-Specific Histone CENH3 in Arabidopsis thaliana
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Meiosis-Specific loading of the Centromere-Specific histone CENH3 in Arabidopsis thaliana", "type"=>"journal", "authors"=>[{"first_name"=>"Maruthachalam", "last_name"=>"Ravi", "scopus_author_id"=>"14326050100"}, {"first_name"=>"Fukashi", "last_name"=>"Shibata", "scopus_author_id"=>"7004862560"}, {"first_name"=>"Joseph S.", "last_name"=>"Ramahi", "scopus_author_id"=>"36844453500"}, {"first_name"=>"Kiyotaka", "last_name"=>"Nagaki", "scopus_author_id"=>"36972615200"}, {"first_name"=>"Changbin", "last_name"=>"Chen", "scopus_author_id"=>"36730767200"}, {"first_name"=>"Minoru", "last_name"=>"Murata", "scopus_author_id"=>"7402129596"}, {"first_name"=>"Simon W L", "last_name"=>"Chan", "scopus_author_id"=>"7404255971"}], "year"=>2011, "source"=>"PLoS Genetics", "identifiers"=>{"scopus"=>"2-s2.0-79959829579", "doi"=>"10.1371/journal.pgen.1002121", "sgr"=>"79959829579", "isbn"=>"1553-7404 (Electronic) 1553-7390 (Linking)", "pmid"=>"21695238", "issn"=>"15537390", "pui"=>"362058274"}, "id"=>"57a7bc28-d79d-37c1-890d-770a2d6d35e9", "abstract"=>"Centromere behavior is specialized in meiosis I, so that sister chromatids of homologous chromosomes are pulled toward the same side of the spindle (through kinetochore mono-orientation) and chromosome number is reduced. Factors required for mono-orientation have been identified in yeast. However, comparatively little is known about how meiotic centromere behavior is specialized in animals and plants that typically have large tandem repeat centromeres. Kinetochores are nucleated by the centromere-specific histone CENH3. Unlike conventional histone H3s, CENH3 is rapidly evolving, particularly in its N-terminal tail domain. Here we describe chimeric variants of CENH3 with alterations in the N-terminal tail that are specifically defective in meiosis. Arabidopsis thaliana cenh3 mutants expressing a GFP-tagged chimeric protein containing the H3 N-terminal tail and the CENH3 C-terminus (termed GFP-tailswap) are sterile because of random meiotic chromosome segregation. These defects result from the specific depletion of GFP-tailswap protein from meiotic kinetochores, which contrasts with its normal localization in mitotic cells. Loss of the GFP-tailswap CENH3 variant in meiosis affects recruitment of the essential kinetochore protein MIS12. Our findings suggest that CENH3 loading dynamics might be regulated differently in mitosis and meiosis. As further support for our hypothesis, we show that GFP-tailswap protein is recruited back to centromeres in a subset of pollen grains in GFP-tailswap once they resume haploid mitosis. Meiotic recruitment of the GFP-tailswap CENH3 variant is not restored by removal of the meiosis-specific cohesin subunit REC8. Our results reveal the existence of a specialized loading pathway for CENH3 during meiosis that is likely to involve the hypervariable N-terminal tail. Meiosis-specific CENH3 dynamics may play a role in modulating meiotic centromere behavior.", "link"=>"http://www.mendeley.com/research/meiosisspecific-loading-centromerespecific-histone-cenh3-arabidopsis-thaliana", "reader_count"=>152, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>10, "Researcher"=>54, "Student > Doctoral Student"=>6, "Student > Ph. D. Student"=>46, "Student > Postgraduate"=>4, "Student > Master"=>11, "Other"=>5, "Student > Bachelor"=>6, "Lecturer"=>2, "Professor"=>6}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>10, "Researcher"=>54, "Student > Doctoral Student"=>6, "Student > Ph. D. Student"=>46, "Student > Postgraduate"=>4, "Student > Master"=>11, "Other"=>5, "Student > Bachelor"=>6, "Lecturer"=>2, "Professor"=>6}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Engineering"=>2, "Biochemistry, Genetics and Molecular Biology"=>14, "Agricultural and Biological Sciences"=>130, "Business, Management and Accounting"=>1, "Sports and Recreations"=>1, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>2}, "Chemistry"=>{"Chemistry"=>1}, "Sports and Recreations"=>{"Sports and Recreations"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>130}, "Business, Management and Accounting"=>{"Business, Management and Accounting"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>14}, "Unspecified"=>{"Unspecified"=>3}}, "reader_count_by_country"=>{"Czech Republic"=>1, "Netherlands"=>1, "Austria"=>1, "United States"=>10, "China"=>1, "Mexico"=>1}, "group_count"=>5}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/79959829579"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/79959829579?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79959829579&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=79959829579&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/79959829579", "dc:identifier"=>"SCOPUS_ID:79959829579", "eid"=>"2-s2.0-79959829579", "dc:title"=>"Meiosis-Specific loading of the Centromere-Specific histone CENH3 in Arabidopsis thaliana", "dc:creator"=>"Ravi M.", "prism:publicationName"=>"PLoS Genetics", "prism:issn"=>"15537390", "prism:eIssn"=>"15537404", "prism:volume"=>"7", "prism:issueIdentifier"=>"6", "prism:pageRange"=>nil, "prism:coverDate"=>"2011-06-01", "prism:coverDisplayDate"=>"June 2011", "prism:doi"=>"10.1371/journal.pgen.1002121", "citedby-count"=>"63", "pubmed-id"=>"21695238", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e1002121", "source-id"=>"4000151808", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosgenetics%2Farticle%3Fid%3D10.1371%252Fjournal.pgen.1002121", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"6", "year"=>"2011", "pdf_views"=>"139", "xml_views"=>"3", "html_views"=>"343"}
  • {"month"=>"7", "year"=>"2011", "pdf_views"=>"100", "xml_views"=>"3", "html_views"=>"412"}
  • {"month"=>"8", "year"=>"2011", "pdf_views"=>"62", "xml_views"=>"1", "html_views"=>"117"}
  • {"month"=>"9", "year"=>"2011", "pdf_views"=>"33", "xml_views"=>"0", "html_views"=>"82"}
  • {"month"=>"10", "year"=>"2011", "pdf_views"=>"47", "xml_views"=>"0", "html_views"=>"116"}
  • {"month"=>"11", "year"=>"2011", "pdf_views"=>"40", "xml_views"=>"5", "html_views"=>"106"}
  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"44", "xml_views"=>"1", "html_views"=>"79"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"31", "xml_views"=>"0", "html_views"=>"97"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"53", "xml_views"=>"1", "html_views"=>"122"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"27", "xml_views"=>"1", "html_views"=>"105"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"98"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"32", "xml_views"=>"0", "html_views"=>"106"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"33", "xml_views"=>"4", "html_views"=>"96"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"23", "xml_views"=>"6", "html_views"=>"107"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"39", "xml_views"=>"0", "html_views"=>"97"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"21", "xml_views"=>"2", "html_views"=>"80"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"40", "xml_views"=>"0", "html_views"=>"85"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"82"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"79"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"2", "html_views"=>"230"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"101"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"45", "xml_views"=>"0", "html_views"=>"81"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"69"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"108"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"28", "xml_views"=>"0", "html_views"=>"85"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"24", "xml_views"=>"2", "html_views"=>"188"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"23", "xml_views"=>"1", "html_views"=>"253"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"30", "xml_views"=>"1", "html_views"=>"198"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"38", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"201"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"32", "xml_views"=>"2", "html_views"=>"273"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"25", "xml_views"=>"1", "html_views"=>"214"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"35", "xml_views"=>"0", "html_views"=>"240"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"212"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"38", "xml_views"=>"0", "html_views"=>"246"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"25", "xml_views"=>"2", "html_views"=>"154"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"306"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"30", "xml_views"=>"3", "html_views"=>"527"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"30", "xml_views"=>"1", "html_views"=>"521"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"15", "xml_views"=>"2", "html_views"=>"303"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"241"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"122"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"36", "xml_views"=>"0", "html_views"=>"87"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"107"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"31", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"82"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"71"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"71"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"69"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"56"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"22", "xml_views"=>"2", "html_views"=>"60"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"81"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"56"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"17", "xml_views"=>"2", "html_views"=>"35"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"45"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"19", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"3", "html_views"=>"25"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"25", "xml_views"=>"1", "html_views"=>"42"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"25", "xml_views"=>"1", "html_views"=>"49"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"24", "xml_views"=>"1", "html_views"=>"48"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"31", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"92", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"23"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/385722", "https://ndownloader.figshare.com/files/385786", "https://ndownloader.figshare.com/files/385829", "https://ndownloader.figshare.com/files/385874", "https://ndownloader.figshare.com/files/385918", "https://ndownloader.figshare.com/files/385989", "https://ndownloader.figshare.com/files/386040", "https://ndownloader.figshare.com/files/386075", "https://ndownloader.figshare.com/files/386102"], "description"=>"<div><p>Centromere behavior is specialized in meiosis I, so that sister chromatids of homologous chromosomes are pulled toward the same side of the spindle (through kinetochore mono-orientation) and chromosome number is reduced. Factors required for mono-orientation have been identified in yeast. However, comparatively little is known about how meiotic centromere behavior is specialized in animals and plants that typically have large tandem repeat centromeres. Kinetochores are nucleated by the centromere-specific histone CENH3. Unlike conventional histone H3s, CENH3 is rapidly evolving, particularly in its N-terminal tail domain. Here we describe chimeric variants of CENH3 with alterations in the N-terminal tail that are specifically defective in meiosis. <em>Arabidopsis thaliana cenh3</em> mutants expressing a GFP-tagged chimeric protein containing the H3 N-terminal tail and the CENH3 C-terminus (termed GFP-tailswap) are sterile because of random meiotic chromosome segregation. These defects result from the specific depletion of GFP-tailswap protein from meiotic kinetochores, which contrasts with its normal localization in mitotic cells. Loss of the GFP-tailswap CENH3 variant in meiosis affects recruitment of the essential kinetochore protein MIS12. Our findings suggest that CENH3 loading dynamics might be regulated differently in mitosis and meiosis. As further support for our hypothesis, we show that GFP-tailswap protein is recruited back to centromeres in a subset of pollen grains in <em>GFP-tailswap</em> once they resume haploid mitosis. Meiotic recruitment of the GFP-tailswap CENH3 variant is not restored by removal of the meiosis-specific cohesin subunit REC8. Our results reveal the existence of a specialized loading pathway for CENH3 during meiosis that is likely to involve the hypervariable N-terminal tail. Meiosis-specific CENH3 dynamics may play a role in modulating meiotic centromere behavior.</p> </div>", "links"=>[], "tags"=>["meiosis-specific", "loading", "centromere-specific", "histone", "cenh3"], "article_id"=>136141, "categories"=>["Cell Biology", "Genetics"], "users"=>["Maruthachalam Ravi", "Fukashi Shibata", "Joseph S. Ramahi", "Kiyotaka Nagaki", "Changbin Chen", "Minoru Murata", "Simon W. L. Chan"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002121.s001", "https://dx.doi.org/10.1371/journal.pgen.1002121.s002", "https://dx.doi.org/10.1371/journal.pgen.1002121.s003", "https://dx.doi.org/10.1371/journal.pgen.1002121.s004", "https://dx.doi.org/10.1371/journal.pgen.1002121.s005", "https://dx.doi.org/10.1371/journal.pgen.1002121.s006", "https://dx.doi.org/10.1371/journal.pgen.1002121.s007", "https://dx.doi.org/10.1371/journal.pgen.1002121.s008", "https://dx.doi.org/10.1371/journal.pgen.1002121.s009"], "stats"=>{"downloads"=>37, "page_views"=>39, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Meiosis_Specific_Loading_of_the_Centromere_Specific_Histone_CENH3_in_Arabidopsis_thaliana_/136141", "title"=>"Meiosis-Specific Loading of the Centromere-Specific Histone CENH3 in <em>Arabidopsis thaliana</em>", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2011-06-09 01:42:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/767643"], "description"=>"<p>Meiocytes from anthers of <i>GFP-CENH3</i> and <i>GFP-tailswap</i> were imaged using identical exposure times. Flattened projections of several stacked images are shown. The GFP-CENH3 protein showed bright fluorescence at kinetochores in all meiocytes (class I). GFP-tailswap protein always showed reduced fluorescence relative to GFP-CENH3. Three classes of GFP fluorescence were observed in <i>GFP-tailswap</i>: faintly visible (class II, 7%), barely detectable (class III, 47%), and undetectable (class IV, 46%).</p>", "links"=>[], "tags"=>["depleted", "kinetochores", "pre-meiotic"], "article_id"=>438014, "categories"=>["Cell Biology", "Genetics", "Plant Biology"], "users"=>["Maruthachalam Ravi", "Fukashi Shibata", "Joseph S. Ramahi", "Kiyotaka Nagaki", "Changbin Chen", "Minoru Murata", "Simon W. L. Chan"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002121.g004", "stats"=>{"downloads"=>4, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_GFP_tailswap_protein_is_depleted_from_kinetochores_during_pre_meiotic_interphase_/438014", "title"=>"GFP-tailswap protein is depleted from kinetochores during pre-meiotic interphase.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-06-09 02:13:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/767929"], "description"=>"<p>Percentage of <i>GFP-CENH3</i> and <i>GFP-tailswap</i> meiocytes showing particular classes of GFP fluorescence at kinetochores.</p>", "links"=>[], "tags"=>["meiocytes", "classes", "gfp", "fluorescence"], "article_id"=>438299, "categories"=>["Cell Biology", "Genetics", "Plant Biology"], "users"=>["Maruthachalam Ravi", "Fukashi Shibata", "Joseph S. Ramahi", "Kiyotaka Nagaki", "Changbin Chen", "Minoru Murata", "Simon W. L. Chan"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002121.g006", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Percentage_of_GFP_CENH3_and_GFP_tailswap_meiocytes_showing_particular_classes_of_GFP_fluorescence_at_kinetochores_/438299", "title"=>"Percentage of <i>GFP-CENH3</i> and <i>GFP-tailswap</i> meiocytes showing particular classes of GFP fluorescence at kinetochores.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-06-09 02:18:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/767534"], "description"=>"<p>a) Centromere DNA FISH from metaphase I in wild type and in <i>GFP-tailswap</i>. Blue = DNA (DAPI), green = centromere DNA FISH (FITC). <i>GFP-tailswap</i> bivalents lack the centromere stretch exerted by the spindle in wild type, and have reduced inter-kinetochore distance. Representative bivalents are magnified in D and H. Scale bars −1 µm. b) Centromere DNA FISH shows random orientation of bivalent chromosomes in <i>GFP-tailswap</i> meiosis I. Blue = DNA (DAPI), green = centromere DNA FISH (FITC). Metaphase I chromosomes are frequently aligned at unusual angles in the mutant (B). Anaphase I chromosomes show random alignment and premature sister chromatid separation (D). Arrows in A and B show presumed orientation of sister centromeres. Arrows in D indicate separated univalents, while arrowheads show intact bivalents. Scale bars −1 µm.</p>", "links"=>[], "tags"=>["inter-kinetochore", "meiotic", "spindle", "defects", "kinetochore"], "article_id"=>437900, "categories"=>["Cell Biology", "Genetics", "Plant Biology"], "users"=>["Maruthachalam Ravi", "Fukashi Shibata", "Joseph S. Ramahi", "Kiyotaka Nagaki", "Changbin Chen", "Minoru Murata", "Simon W. L. Chan"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002121.g003", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Reduced_inter_kinetochore_distance_and_meiotic_spindle_defects_suggest_lack_of_kinetochore_function_in_GFP_tailswap_/437900", "title"=>"Reduced inter-kinetochore distance and meiotic spindle defects suggest lack of kinetochore function in <i>GFP-tailswap</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-06-09 02:11:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/767294"], "description"=>"<p>a) CENH3 transgenes tested for fertility in a <i>cenh3-1</i> homozygous mutant background. The male fertility was examined by Alexander staining. Viable pollen stains pink/red. Female fertility was judged by differential intereference contrast (DIC) microscopy of embryo sacs from at least 100 cleared mature ovules per genotype (<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002121#pgen.1002121.s001\" target=\"_blank\">Figure S1A</a>). Single cell arrested ovules and ovules without an embryo sac (<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002121#pgen.1002121.s001\" target=\"_blank\">Figure S1B</a>) were counted as non-viable, and ovules with 7–8 celled embryo sacs (<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002121#pgen.1002121.s001\" target=\"_blank\">Figure S1B</a>) were counted as viable. Viable ovules may be haploid or aneuploid. b) Male meiotic chromosome spreads from wild type and <i>GFP-tailswap</i> plants. Metaphase I bivalents in the mutant are oval/round in shape, lacking the rhombus shape that indicates tension in wild type (compare A and F). Some metaphase I cells showed chromosomes that failed to congress to the spindle midzone (arrowed in K). Chromosome segregation at anaphase I is random in <i>GFP-tailswap</i> (G to I, L to N). Asynchronous homolog separation was seen at anaphase I (arrowed in G), and premature sister chromatid separation was also seen in meiosis I (arrowed in N). Decondensation at interkinesis was frequently delayed, especially for lagging chromosomes near the spindle midzone (arrowed in J, O). Metaphase II cells in the mutant show random chromosome alignment (U, Z). U shows one univalent (arrowed) and four bivalents plus the remaining univalent on the other side of the cell. Anaphase II chromosome segregation is random (V–X, AA–AC). Tetrad equivalent stages in <i>GFP-tailswap</i> (Y, AD) show several small nuclei instead of the expected four uniform nuclei seen in wild type. Scale bars −1 µm.</p>", "links"=>[], "tags"=>["cenh3", "n-terminal", "leads", "defects", "meiotic", "chromosome"], "article_id"=>437658, "categories"=>["Cell Biology", "Genetics", "Plant Biology"], "users"=>["Maruthachalam Ravi", "Fukashi Shibata", "Joseph S. Ramahi", "Kiyotaka Nagaki", "Changbin Chen", "Minoru Murata", "Simon W. L. Chan"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002121.g001", "stats"=>{"downloads"=>2, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Altering_the_CENH3_N_terminal_tail_domain_leads_to_defects_in_meiotic_chromosome_segregation_/437658", "title"=>"Altering the CENH3 N-terminal tail domain leads to defects in meiotic chromosome segregation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-06-09 02:07:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/768056"], "description"=>"<p>GFP-CENH3, GFP-tailswap and MIS12 proteins were immunolocalized in anthers during the pachytene stage of meiosis with anti-GFP and anti-MIS12 antibodies. Somatic cells from the same anther are shown as a control. GFP-CENH3 and MIS12 were visualized at both meiotic and somatic kinetochores of <i>GFP-CENH3</i> plants. In <i>GFP-tailswap</i> plants, GFP-tailswap and MIS12 were both undetectable in <i>GFP-tailswap</i> meiotic kinetochores but can be seen in somatic kinetochores. Scale bars −5 µm.</p>", "links"=>[], "tags"=>["gfp-tailswap", "meiotic", "kinetochores", "causes"], "article_id"=>438421, "categories"=>["Cell Biology", "Genetics", "Plant Biology"], "users"=>["Maruthachalam Ravi", "Fukashi Shibata", "Joseph S. Ramahi", "Kiyotaka Nagaki", "Changbin Chen", "Minoru Murata", "Simon W. L. Chan"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002121.g007", "stats"=>{"downloads"=>1, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Depletion_of_GFP_tailswap_protein_from_meiotic_kinetochores_causes_removal_of_MIS12_/438421", "title"=>"Depletion of GFP-tailswap protein from meiotic kinetochores causes removal of MIS12.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-06-09 02:20:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/768230"], "description"=>"<p>Anther meiocytes from wild type and <i>GFP-tailswap</i> plants were stained with anti-tubulin antibodies. Metaphase I spindles are disorganized and longer in <i>GFP-tailswap</i>, and may contain fewer microtubules (E and I). Meiosis II cells in <i>GFP-tailswap</i> often contain more than two spindles (F–G, J–K). Spindle appearance and orientation are disordered, and may fail to include some chromosomes (arrowed in G). Tetrad equivalent cells (H, L) lack the radial microtubule system that surrounds the four haploid nuclei in wild type. Scale bars −5 µm.</p>", "links"=>[], "tags"=>["genetics and genomics", "plant biology", "cell biology"], "article_id"=>438598, "categories"=>["Cell Biology", "Genetics", "Plant Biology"], "users"=>["Maruthachalam Ravi", "Fukashi Shibata", "Joseph S. Ramahi", "Kiyotaka Nagaki", "Changbin Chen", "Minoru Murata", "Simon W. L. Chan"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002121.g008", "stats"=>{"downloads"=>1, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Immunolocalization_of_tubulin_in_wild_type_and_GFP_tailswap_meiocytes_/438598", "title"=>"Immunolocalization of α-tubulin in wild type and <i>GFP-tailswap</i> meiocytes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-06-09 02:23:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/767781"], "description"=>"<p>a) Dynamics of kinetochore GFP-CENH3 and GFP-tailswap proteins during meiosis were visualized in anthers. Flattened projections of several stacked images are shown. GFP-CENH3 is visible at uniform intensity at kinetochores throughout meiosis. GFP-tailswap is barely detectable or undetectable in leptotene and zygotene (comparable to class III and class IV in <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002121#pgen-1002121-g004\" target=\"_blank\">Figure 4</a>). In pachytene and subsequent stages, we did not detect kinetochore GFP fluorescence in <i>GFP-tailswap</i> meiocytes.</p>", "links"=>[], "tags"=>["gfp-tailswap", "kinetochores", "continues", "progressively"], "article_id"=>438147, "categories"=>["Cell Biology", "Genetics", "Plant Biology"], "users"=>["Maruthachalam Ravi", "Fukashi Shibata", "Joseph S. Ramahi", "Kiyotaka Nagaki", "Changbin Chen", "Minoru Murata", "Simon W. L. Chan"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002121.g005", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Depletion_of_GFP_tailswap_protein_from_kinetochores_continues_progressively_during_meiosis_/438147", "title"=>"Depletion of GFP-tailswap protein from kinetochores continues progressively during meiosis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-06-09 02:15:47"}
  • {"files"=>["https://ndownloader.figshare.com/files/768354"], "description"=>"<p>Chromosome spreads from male meiosis in <i>rec8 spo11-1</i> and <i>rec8 spo11-1 cenh3-1 GFP-tailswap</i>. Anaphase I in <i>rec8 spo11-1</i> shows orderly separation of sister chromatids that is similar to mitosis, because the <i>rec8</i> mutation converts kinetochores to a mitosis-like behavior (A, D). Anaphase I in <i>rec8 spo11-1 cenh3-1 GFP-tailswap</i> shows random segregation of univalent chromosomes (B, C, E, F). This is consistent with the observation that removing REC8 does not restore loading of the GFP-CENH3 protein (<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002121#pgen.1002121.s006\" target=\"_blank\">Figure S6</a>).</p>", "links"=>[], "tags"=>["meiosis-specific", "cohesin", "rec8", "meiotic", "kinetochore"], "article_id"=>438717, "categories"=>["Cell Biology", "Genetics", "Plant Biology"], "users"=>["Maruthachalam Ravi", "Fukashi Shibata", "Joseph S. Ramahi", "Kiyotaka Nagaki", "Changbin Chen", "Minoru Murata", "Simon W. L. Chan"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002121.g009", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Removing_the_meiosis_specific_cohesin_REC8_does_not_restore_meiotic_kinetochore_function_in_GFP_tailswap_/438717", "title"=>"Removing the meiosis-specific cohesin REC8 does not restore meiotic kinetochore function in <i>GFP-tailswap</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-06-09 02:25:17"}
  • {"files"=>["https://ndownloader.figshare.com/files/767419"], "description"=>"<p>Immunolocalization of alpha-tubulin outlines the nuclear envelope in microspores of <i>GFP-CENH3</i> and <i>GFP-tailswap</i> pollen (A–F). <i>GFP-tailswap</i> pollen contains multiple micronuclei. Centromere DNA FISH shows that micronuclei contain 1–2 chromosomes each, as opposed to 5 chromosomes in a normal <i>A. thaliana</i> haploid pollen genome (G–L). The pollen grain shown in J–L has three micronuclei. Two contain one chromosome each, while the third contains two chromosomes.</p>", "links"=>[], "tags"=>["centromere", "meiosis", "causes", "micronuclei"], "article_id"=>437787, "categories"=>["Cell Biology", "Genetics", "Plant Biology"], "users"=>["Maruthachalam Ravi", "Fukashi Shibata", "Joseph S. Ramahi", "Kiyotaka Nagaki", "Changbin Chen", "Minoru Murata", "Simon W. L. Chan"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002121.g002", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Lack_of_centromere_function_in_meiosis_causes_micronuclei_to_form_in_GFP_tailswap_pollen_/437787", "title"=>"Lack of centromere function in meiosis causes micronuclei to form in <i>GFP-tailswap</i> pollen.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-06-09 02:09:47"}
  • {"files"=>["https://ndownloader.figshare.com/files/768439"], "description"=>"<p>a) Microspores of wild type, <i>GFP-CENH3</i> and <i>GFP-tailswap</i>. Tetrad equivalent stages in <i>GFP-tailswap</i> do not show any GFP fluorescence at meiotic kinetochores (<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002121#pgen.1002121.s004\" target=\"_blank\">Figure S4</a>). However, GFP-tailswap protein reloads onto kinetochores in a small fraction of microspores, when haploid mitotic divisions are expected to resume. b) Frequency of GFP-positive and -negative microspores from <i>GFP-CENH3</i> and <i>GFP-tailswap</i> plants. c) The number of GFP foci in each GFP-positive spore is shown.</p>", "links"=>[], "tags"=>["reloads", "centromeres", "mitosis"], "article_id"=>438806, "categories"=>["Cell Biology", "Genetics", "Plant Biology"], "users"=>["Maruthachalam Ravi", "Fukashi Shibata", "Joseph S. Ramahi", "Kiyotaka Nagaki", "Changbin Chen", "Minoru Murata", "Simon W. L. Chan"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002121.g010", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_GFP_tailswap_protein_reloads_onto_centromeres_after_meiosis_when_mitosis_resumes_/438806", "title"=>"GFP-tailswap protein reloads onto centromeres after meiosis, when mitosis resumes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-06-09 02:26:46"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"34", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"13", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"17", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"23", "full-text"=>"11", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"19", "full-text"=>"17", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"8", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"15", "full-text"=>"10", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"37", "full-text"=>"7", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"31", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"39", "full-text"=>"11", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"37", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"19", "full-text"=>"10", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"19", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"28", "full-text"=>"14", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"7", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"16", "full-text"=>"14", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"6", "pdf"=>"13", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"22", "full-text"=>"9", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"20", "full-text"=>"20", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2011-01-01T00:00:00Z", "end_date"=>"2011-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[289, 559, 699, 817, 931, 1031, 1127, 1214, 1297, 1370, 1444, 1515, 1584, 1656, 1726, 1797, 1862, 1930, 1996, 2061, 2125, 2190, 2250, 2311, 2373, 2435, 2496, 2563, 2630, 2692, 2760, 2824, 2898, 2960, 3019, 3089, 3143]}]}
Loading … Spinner
There are currently no alerts