Drosophila Araucan and Caupolican Integrate Intrinsic and Signalling Inputs for the Acquisition by Muscle Progenitors of the Lateral Transverse Fate
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{"title"=>"Drosophila araucan and caupolican integrate intrinsic and signalling inputs for the acquisition by muscle progenitors of the lateral transverse fate", "type"=>"journal", "authors"=>[{"first_name"=>"Marta", "last_name"=>"Carrasco-Rando", "scopus_author_id"=>"13807511100"}, {"first_name"=>"Antonio S.", "last_name"=>"Tutor", "scopus_author_id"=>"10045249200"}, {"first_name"=>"Silvia", "last_name"=>"Prieto-Sánchez", "scopus_author_id"=>"25959310400"}, {"first_name"=>"Esther", "last_name"=>"González-Pérez", "scopus_author_id"=>"35082003700"}, {"first_name"=>"Natalia", "last_name"=>"Barrios", "scopus_author_id"=>"15750112100"}, {"first_name"=>"Annalisa", "last_name"=>"Letizia", "scopus_author_id"=>"16304203200"}, {"first_name"=>"Paloma", "last_name"=>"Martín", "scopus_author_id"=>"34768744200"}, {"first_name"=>"Sonsoles", "last_name"=>"Campuzano", "scopus_author_id"=>"7004398097"}, {"first_name"=>"Mar", "last_name"=>"Ruiz-Gómez", "scopus_author_id"=>"7004556501"}], "year"=>2011, "source"=>"PLoS Genetics", "identifiers"=>{"issn"=>"15537390", "pmid"=>"21811416", "scopus"=>"2-s2.0-79960962033", "doi"=>"10.1371/journal.pgen.1002186", "pui"=>"362246898", "isbn"=>"1553-7404 (Electronic)\\r1553-7390 (Linking)", "sgr"=>"79960962033"}, "id"=>"cd93d1e6-9a40-311f-bfbc-7656e93aeb3f", "abstract"=>"A central issue of myogenesis is the acquisition of identity by individual muscles. In Drosophila, at the time muscle progenitors are singled out, they already express unique combinations of muscle identity genes. This muscle code results from the integration of positional and temporal signalling inputs. Here we identify, by means of loss-of-function and ectopic expression approaches, the Iroquois Complex homeobox genes araucan and caupolican as novel muscle identity genes that confer lateral transverse muscle identity. The acquisition of this fate requires that Araucan/Caupolican repress other muscle identity genes such as slouch and vestigial. In addition, we show that Caupolican-dependent slouch expression depends on the activation state of the Ras/Mitogen Activated Protein Kinase cascade. This provides a comprehensive insight into the way Iroquois genes integrate in muscle progenitors, signalling inputs that modulate gene expression and protein activity.", "link"=>"http://www.mendeley.com/research/drosophila-araucan-caupolican-integrate-intrinsic-signalling-inputs-acquisition-muscle-progenitors-l", "reader_count"=>19, "reader_count_by_academic_status"=>{"Student > Doctoral Student"=>2, "Researcher"=>5, "Student > Ph. D. Student"=>2, "Student > Postgraduate"=>2, "Student > Master"=>6, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Student > Doctoral Student"=>2, "Researcher"=>5, "Student > Ph. D. Student"=>2, "Student > Postgraduate"=>2, "Student > Master"=>6, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>15, "Neuroscience"=>1}, "reader_count_by_subdiscipline"=>{"Neuroscience"=>{"Neuroscience"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>15}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"Ireland"=>1, "Germany"=>1}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/753824"], "description"=>"<p>All images show a detail of an embryonic wild-type abdominal hemisegment stained with antibodies against Caup (green) and different muscle identity proteins. Images show a ventral view of the embryo, with the exception of B and C that correspond to lateral views. (A–C) Stage 11 embryos. (A) <i>caup</i> and <i>Kr</i> (red) are co-expressed in a lateral transverse promuscular cluster (C<sub>LTS</sub>). (B–C) <i>caup</i> is co-expressed with <i>Kr</i> (blue) in progenitors of LT muscles (P<sub>LT1/LT2</sub> and P<sub>LT3/LT4</sub>, B) and with <i>slou/</i>S59 (red) in DT1/DO3 progenitor (P<sub>DT1/DO3</sub>, C). (D) Late stage 12 embryo co-expressing <i>caup</i> and <i>lb</i> in the SBM founder (F<sub>SBM</sub>). (E) Stage 12 embryo showing co-expression of <i>caup</i> with <i>slou/</i>S59 (red) in DT1 founder (F<sub>DT1</sub>) and with Kr (blue) in LTs founders (F<sub>LT1–4</sub>). The position of LL1, LL1sib and VA1–3 founders (F<sub>LL1</sub>, F<sub>LL1sib</sub>, F<sub>VA1–3</sub>) and the ventral adult muscle precursor are also indicated. (F) Schematic representation of <i>ara/caup</i> expression in the LTs, DT1 and SBM lineages (SBM lineage as revised in <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002186#pgen.1002186-Knirr1\" target=\"_blank\">[17]</a>). LaPs, lateral adult muscle precursors; PC, promuscular cluster; P, muscle progenitor; Fs, founder myoblasts.</p>", "links"=>[], "tags"=>["caup", "muscles"], "article_id"=>424192, "categories"=>["Genetics", "Developmental Biology"], "users"=>["Marta Carrasco-Rando", "Antonio S. Tutor", "Silvia Prieto-Sánchez", "Esther González-Pérez", "Natalia Barrios", "Annalisa Letizia", "Paloma Martín", "Sonsoles Campuzano", "Mar Ruiz-Gómez"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002186.g002", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Onset_of_Caup_expression_in_muscles_in_relation_to_other_muscle_identity_genes_/424192", "title"=>"Onset of Caup expression in muscles in relation to other muscle identity genes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-07-21 01:09:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/753940"], "description"=>"<p>(A–D) Latero-ventral region of stage 16 wild-type (A), <i>Df(3L)iro<sup>DFM3</sup></i> (B), <i>Df(3L)iro<sup>EGP6</sup></i> (C) and stage 15 <i>Df(3L)iro<sup>DFM3</sup> mef-2GAL4::UAS-ara</i> (D) embryos stained with anti- Tropomyosin antibody (green). The position of ventral wild-type LT muscle tips and LT attachment sites are marked with arrowheads and brackets, respectively. Note the absence of muscles with LT morphology and insertions at LT attachment sites, and the presence of morphologically normal DT1 and SBM muscles (arrows) in the mutant backgrounds (B, C). Asterisks indicate morphological abnormal latero-ventral muscles in these embryos. This phenotype is rescued by mesodermal <i>ara</i> expression with the pan-mesodermal driver <i>mef2-GAL4</i> (D). (E) Quantification of phenotypes produced by the loss of <i>ara/caup</i> in LT muscles. * Refers to changes in shape, orientation or attachment sites; n, numbers of hemisegments analysed (stages 14–16); -, not determined.</p>", "links"=>[], "tags"=>["phenotypes", "mutant"], "article_id"=>424301, "categories"=>["Genetics", "Developmental Biology"], "users"=>["Marta Carrasco-Rando", "Antonio S. Tutor", "Silvia Prieto-Sánchez", "Esther González-Pérez", "Natalia Barrios", "Annalisa Letizia", "Paloma Martín", "Sonsoles Campuzano", "Mar Ruiz-Gómez"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002186.g003", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Muscle_phenotypes_of_Iro_C_mutant_embryos_/424301", "title"=>"Muscle phenotypes of <i>Iro-C</i> mutant embryos.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-07-21 01:11:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/754219"], "description"=>"<p>(A) Diagram of the 2 Kb long <i>slou</i> promoter region (from −1828 to +153 nt) used to drive Luciferase expression. This fragment contains two putative binding sites for Ara/Caup, BS1 and BS2. (B) Effect of increasing amounts of Caup-HA on the Luciferase activity driven by <i>slou</i> promoter in the absence (blue bars) and presence (red bars) of PD98059 MEK1 inhibitor. (C) Representative western blots of lysates of S2 cells expressing increasing amounts of Caup (upper panel) in the absence and presence of PD98059, showing the state of activation of the Ras/MAPK cascade (middle panel) and Tubulin expression as loading control (lower panel). (D–F) Mutagenesis analysis of <i>slou</i> regulatory region. (D) Binding of Caup to the indicated <i>slou</i> regulatory fragments, containing BS1 determined by EMSA. Binding of Caup to wild-type fragment resulted in the formation of complexes with reduced mobility (asterisk in lane 4), which was more evident in the presence of increased amounts of Caup (asterisk in lane 7). No shift was observed when fragments devoid of BS1 (Δ BS1, lanes 5, 8) or point-mutated (Mut BS1, lanes 6, 9) were used or in the absence of Caup (lanes 1–3). (E, F) Effect of Caup-HA (1 µg) on the Luciferase activity driven by wt and mutated (BS1*, BS2*) <i>slou</i> promoter regions in the absence (E) and presence (F) of PD98059 inhibitor. Statistical analyses for Luciferase assays were performed using the paired two-tailed Student's t-test. The data are presented as means ± S.E.M. of 3 independent experiments. *P<0.05, **P<0.001 compared to basal (B) or wt (E, F) conditions.</p>", "links"=>[], "tags"=>["caup", "modulation"], "article_id"=>424588, "categories"=>["Genetics", "Developmental Biology"], "users"=>["Marta Carrasco-Rando", "Antonio S. Tutor", "Silvia Prieto-Sánchez", "Esther González-Pérez", "Natalia Barrios", "Annalisa Letizia", "Paloma Martín", "Sonsoles Campuzano", "Mar Ruiz-Gómez"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002186.g006", "stats"=>{"downloads"=>4, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Direct_interaction_of_Caup_with_slou_regulatory_region_and_its_modulation_by_the_Ras_MAPK_pathway_/424588", "title"=>"Direct interaction of Caup with <i>slou</i> regulatory region and its modulation by the Ras/MAPK pathway.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-07-21 01:16:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/754445"], "description"=>"<p>In the wild-type LT3–4 promuscular cluster, where Ras/MAPK signalling is inactive, Caup represses <i>slou</i> since in embryos mutant for <i>ara/caup</i> (<i>Df(3L)iro<sup>EGP6</sup></i>), the absence of Caup allows <i>slou</i> activation in this cluster and the consequent transformation of LT3–4 muscles to VA1–2 muscles. In the wild-type Caup is absent from the DER-dependent VA1–2 cluster that expresses <i>slou</i>. Ectopic expression of Caup in the VA1–2 lineages using <i>Con-GAL4</i> (active after founder segregation when MAPK signalling is extinguished) represses <i>slou</i> in VA2. On the contrary, <i>Con-GAL4</i> driven expression of Caup together with the activated form of Ras alleviates Caup-dependent <i>slou</i> repression in the VA2 muscle.</p>", "links"=>[], "tags"=>["activation", "signalling", "cascade", "lt", "va"], "article_id"=>424808, "categories"=>["Genetics", "Developmental Biology"], "users"=>["Marta Carrasco-Rando", "Antonio S. Tutor", "Silvia Prieto-Sánchez", "Esther González-Pérez", "Natalia Barrios", "Annalisa Letizia", "Paloma Martín", "Sonsoles Campuzano", "Mar Ruiz-Gómez"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002186.g008", "stats"=>{"downloads"=>3, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_the_state_of_activation_of_the_Ras_MAPK_signalling_cascade_on_the_regulation_of_slou_by_Ara_Caup_in_LT_and_VA_lineages_/424808", "title"=>"Effect of the state of activation of the Ras/MAPK signalling cascade on the regulation of <i>slou</i> by Ara/Caup in LT and VA lineages.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-07-21 01:20:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/379444", "https://ndownloader.figshare.com/files/379491", "https://ndownloader.figshare.com/files/379541", "https://ndownloader.figshare.com/files/379586", "https://ndownloader.figshare.com/files/379639", "https://ndownloader.figshare.com/files/379679"], "description"=>"<div><p>A central issue of myogenesis is the acquisition of identity by individual muscles. In <em>Drosophila</em>, at the time muscle progenitors are singled out, they already express unique combinations of muscle identity genes. This muscle code results from the integration of positional and temporal signalling inputs. Here we identify, by means of loss-of-function and ectopic expression approaches, the Iroquois Complex homeobox genes <em>araucan</em> and <em>caupolican</em> as novel muscle identity genes that confer lateral transverse muscle identity. The acquisition of this fate requires that Araucan/Caupolican repress other muscle identity genes such as <em>slouch</em> and <em>vestigial</em>. In addition, we show that Caupolican-dependent <em>slouch</em> expression depends on the activation state of the Ras/Mitogen Activated Protein Kinase cascade. This provides a comprehensive insight into the way Iroquois genes integrate in muscle progenitors, signalling inputs that modulate gene expression and protein activity.</p> </div>", "links"=>[], "tags"=>["araucan", "caupolican", "intrinsic", "signalling", "inputs", "progenitors", "lateral", "transverse"], "article_id"=>134984, "categories"=>["Genetics", "Developmental Biology"], "users"=>["Marta Carrasco-Rando", "Antonio S. Tutor", "Silvia Prieto-Sánchez", "Esther González-Pérez", "Natalia Barrios", "Annalisa Letizia", "Paloma Martín", "Sonsoles Campuzano", "Mar Ruiz-Gómez"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002186.s001", "https://dx.doi.org/10.1371/journal.pgen.1002186.s002", "https://dx.doi.org/10.1371/journal.pgen.1002186.s003", "https://dx.doi.org/10.1371/journal.pgen.1002186.s004", "https://dx.doi.org/10.1371/journal.pgen.1002186.s005", "https://dx.doi.org/10.1371/journal.pgen.1002186.s006"], "stats"=>{"downloads"=>5, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Drosophila_Araucan_and_Caupolican_Integrate_Intrinsic_and_Signalling_Inputs_for_the_Acquisition_by_Muscle_Progenitors_of_the_Lateral_Transverse_Fate/134984", "title"=>"<em>Drosophila</em> Araucan and Caupolican Integrate Intrinsic and Signalling Inputs for the Acquisition by Muscle Progenitors of the Lateral Transverse Fate", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2011-07-21 01:23:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/754316"], "description"=>"<p>(A) Schematic drawing of muscles expressing <i>Con</i> in abdominal hemisegments. (B–E) Lateral views of abdominal hemisegments of stage 15–16 wild type (B), <i>Df(3L)iro<sup>DFM3</sup></i> (C), <i>Con-GAL4::UAS-caup<sup>HA</sup></i> (D) and <i>Con-GAL4::UAS-caupHA; UAS-ras<sup>V12</sup></i> (E) embryos, stained with S59 antibody. Note the presence of an ectopic VA2 muscle (VA2*) in <i>Df(3L)iro<sup>DFM3</sup></i> (C), the absence of <i>slou</i> in VA2 when <i>caup</i> is ectopically expressed in this muscle (arrow, D, see also F-F″), and the failure of Caup to repress <i>slou/</i>S59 in VA2 muscle in the presence of the activated form of Ras, <i>ras<sup>V12</sup></i> (E). (F-G″) Lateral views of stage 15–16 <i>Con-GAL4::UAS-caupHA; UAS-GFP</i> (F-F″) and <i>Con-GAL4::UAS-caupHA; UAS-slou</i> (G-G″) embryos stained with the indicated antibodies. Note that co-expression of <i>caup</i> and <i>slou</i> in VA2 does not appreciably modify the morphology of the muscle (arrows in G-G″). As an internal control co-expression of <i>UAS-caup</i> and <i>UAS-GFP</i> still repressed endogenous <i>slou</i> and prevented the VA2 fate (F-F″). (H) Close-up of a lateral transverse promuscular cluster (outlined) in a stage 11 wild-type embryo showing co-expression of Caup (red) and Kr (blue) in all cells of the clusters. Note that the activation of the Ras/MAPK cascade (dpErk, green) only takes place at low levels in the segregating progenitor (yellow arrowhead) but not in the rest of the cluster. (I) Close-up of LT cluster in <i>twist-GAL4; 24B-GAL4::UAS- ras<sup>V12</sup></i> stage 11 embryo. Early activation of the Ras/MAPK pathway prevents the repression of <i>slou</i> by Caup in the LT cluster. (J) Close-up of the dorsal mesoderm of a <i>mef2-GAL4::UAS-ara</i> stage 15 embryo showing ectopic expression of <i>slou</i> in <i>eve</i>-expressing muscles.</p>", "links"=>[], "tags"=>["modulates", "transcriptional", "caup"], "article_id"=>424680, "categories"=>["Genetics", "Developmental Biology"], "users"=>["Marta Carrasco-Rando", "Antonio S. Tutor", "Silvia Prieto-Sánchez", "Esther González-Pérez", "Natalia Barrios", "Annalisa Letizia", "Paloma Martín", "Sonsoles Campuzano", "Mar Ruiz-Gómez"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002186.g007", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Ras_MAPK_modulates_the_transcriptional_activity_of_Caup_on_slou_during_myogenesis_/424680", "title"=>"Ras/MAPK modulates the transcriptional activity of Caup on <i>slou</i> during myogenesis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-07-21 01:18:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/754027"], "description"=>"<p>(A-B′) Late stage 12 control (A-A″) and <i>rP298;;Df(3L)iro<sup>EGP6</sup></i> sibling embryos (B, B′) stained with anti-ßgal (red), anti-Slou/S59 (green) and anti-Kr (blue) antibodies. ßgal staining is used as a marker for founders (<i>rP298</i> line) and the white rectangle in A marks the individual segment shown in A′, A″. (A′, B) Drawings indicating the relative position of the founders visualised in the corresponding (A″, B′) confocal images. The founders expressing <i>Kr</i> or <i>slou</i>/S59 are labelled by their muscle's acronyms. Note that although founder segregation is unaffected in <i>Df(3L)iro<sup>EGP6</sup></i> embryos, the specification of LT founders is altered (B, B′). Thus, two of the LT founders (LT3–4* in B, B′), marked by expression of <i>Kr</i>, also express <i>slou</i>/S59, a property exclusive of the VA1–2 founders (see insets below for details of LT founders, the asterisks mark VT1 founder, that expresses <i>slou</i>/S59 but not <i>Kr</i>. Note that Kr is disappearing from LT1 and 3). All panels show Z projections of several consecutive confocal sections with the exception of A″ that corresponds to a combination of two Z projections, one lateral, as the one shown in B′, and other rotated ventrally to show VAs founders. For muscle nomenclature other than ventral adult precursor (VaP) see <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002186#pgen.1002186-Bate1\" target=\"_blank\">[1]</a>.</p>", "links"=>[], "tags"=>["lt", "founders", "mutant"], "article_id"=>424395, "categories"=>["Genetics", "Developmental Biology"], "users"=>["Marta Carrasco-Rando", "Antonio S. Tutor", "Silvia Prieto-Sánchez", "Esther González-Pérez", "Natalia Barrios", "Annalisa Letizia", "Paloma Martín", "Sonsoles Campuzano", "Mar Ruiz-Gómez"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002186.g004", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Changes_of_fate_in_LT_founders_of_Iro_C_mutant_embryos_/424395", "title"=>"Changes of fate in LT founders of <i>Iro-C</i> mutant embryos.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-07-21 01:13:15"}
  • {"files"=>["https://ndownloader.figshare.com/files/753689"], "description"=>"<p>Wild type embryos of the indicated developmental stages were hybridized with <i>caup</i> (A, A′, B, B′, C, E, E′) or <i>ara</i> (F) riboprobes or sectioned after anti-Caup antibody staining (A″, A′″, B″, B′″, C′, D, D′, E″). (A-A′″) <i>caup</i> is expressed in the visceral mesoderm at early stage 11 (arrowheads, A and A′ show the same embryo with different focus as shown in the inset). (B-B′″) At mid stage 11 <i>caup</i> is expressed in the visceral mesoderm (arrowheads) and in the lateral ectoderm (arrows). Asterisks in A and B point to the primordium of the proventriculus. A′″, B′″ close-ups of the images shown in A″ and B″, respectively. (C-C′) Early stage 12/late stage 11 embryos. (C) <i>caup</i> is expressed in the lateral ectoderm (arrowhead) and in groups of mesodermal cells (arrow). (C′) Cross-section showing <i>caup</i> expression in ectodermal cells (Ec), visceral mesoderm (Vms) and promuscular clusters (Cl). (D, D′) At stage 12 <i>caup</i> is expressed in individual muscle progenitors (P in D) and slightly later in both founders (Fs) derived from the division of progenitors (D′). (E-E″) At stage 13 Caup is detected in a lateral stripe of ectodermal cells (arrowheads in E, E′, Ec in E″) and in muscle precursors (arrows in E, E′, M in E″). (F) Stage 15 embryo showing expression of <i>ara</i> in the ectoderm and in mature muscles. (G) Stage 15 embryos doubled stained with anti-Caup (green) and antibodies against Con, Slou or Ladybird (red). <i>caup</i> is co-expressed with <i>Con</i> in LT1–4 muscles, with <i>slou</i> in DT1 and with <i>lb</i> in SBM. The drawing scheme summarises the wild type patterns of expression of <i>caup</i> (green), <i>slou</i> (red), <i>lb</i> (yellow) and <i>Con</i> (black contour line) in relation to the wild type complement of abdominal muscles. For muscle nomenclature see <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002186#pgen.1002186-Bate1\" target=\"_blank\">[1]</a>.</p>", "links"=>[], "tags"=>["genetics and genomics", "developmental biology"], "article_id"=>424054, "categories"=>["Genetics", "Developmental Biology"], "users"=>["Marta Carrasco-Rando", "Antonio S. Tutor", "Silvia Prieto-Sánchez", "Esther González-Pérez", "Natalia Barrios", "Annalisa Letizia", "Paloma Martín", "Sonsoles Campuzano", "Mar Ruiz-Gómez"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002186.g001", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Pattern_of_expression_of_ara_and_caup_during_myogenesis_/424054", "title"=>"Pattern of expression of <i>ara</i> and <i>caup</i> during myogenesis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-07-21 01:07:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/754136"], "description"=>"<p>(A) Summary of identity codes for promuscular clusters (Cl), progenitors (P) and muscles missing or duplicated in <i>ara/caup</i> mutants, indicated by a colour code. (B) Schematic drawings of the body wall muscles in wild type abdominal hemisegments, depicting the muscles that express the marker indicated on top. (C) Stage 14 <i>Df(3L)iro<sup>DFM3</sup></i> embryo showing a duplication of LL1 fate in the lateral region, pointed by an arrow (LL1*). As shown in the corresponding schemes, LL1 is the only muscle that co-expresses <i>Kr</i> (green) and <i>vg</i> (red) in the lateral region. (D, E) Double-staining with anti-Kr (green) and anti-Slou/S59 (red) antibodies in stage 14 wild-type (D) and <i>Df(3L)iro<sup>DFM3</sup></i> (E) embryos, showing duplication of VA2 fate in the mutant embryo that co-expresses <i>Kr</i> and <i>slou/</i>S59 (VA2*). (F) At stage 14 two VA2-like muscle precursors expressing <i>Kr</i> and <i>Poxm</i> and two <i>Poxm</i>-expressing VA1-like precursors are observed in <i>Df(3L)iro<sup>DFM3</sup></i> embryos. (G) The duplicated muscles are clearly visualised at stage 15, when <i>Poxm</i> expression is still clear in VA1 but fading in VA2 muscles. Note the presence of two muscles expressing higher levels of <i>Poxm</i> (green, VA1 and VA1*) next to two fibres co-expressing low levels of <i>Poxm</i> and <i>slou/</i>S59 (red) in a <i>Df(3L)iro<sup>DFM3</sup></i> embryo.</p>", "links"=>[], "tags"=>["transformations"], "article_id"=>424502, "categories"=>["Genetics", "Developmental Biology"], "users"=>["Marta Carrasco-Rando", "Antonio S. Tutor", "Silvia Prieto-Sánchez", "Esther González-Pérez", "Natalia Barrios", "Annalisa Letizia", "Paloma Martín", "Sonsoles Campuzano", "Mar Ruiz-Gómez"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002186.g005", "stats"=>{"downloads"=>3, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Muscle_fate_transformations_in_Df_3L_iro_DFM3_embryos_/424502", "title"=>"Muscle fate transformations in <i>Df(3L)iro<sup>DFM3</sup></i> embryos.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-07-21 01:15:02"}

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Relative Metric

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