The SUN Protein Mps3 Is Required for Spindle Pole Body Insertion into the Nuclear Membrane and Nuclear Envelope Homeostasis
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{"title"=>"The SUN protein Mps3 is required for spindle pole body insertion into the nuclear membrane and nuclear envelope homeostasis", "type"=>"journal", "authors"=>[{"first_name"=>"Jennifer M.", "last_name"=>"Friederichs", "scopus_author_id"=>"37116887000"}, {"first_name"=>"Suman", "last_name"=>"Ghosh", "scopus_author_id"=>"24516715600"}, {"first_name"=>"Christine J.", "last_name"=>"Smoyer", "scopus_author_id"=>"53464447000"}, {"first_name"=>"Scott", "last_name"=>"McCroskey", "scopus_author_id"=>"55574147200"}, {"first_name"=>"Brandon D.", "last_name"=>"Miller", "scopus_author_id"=>"54585661800"}, {"first_name"=>"Kyle J.", "last_name"=>"Weaver", "scopus_author_id"=>"54586060700"}, {"first_name"=>"Kym M.", "last_name"=>"Delventhal", "scopus_author_id"=>"24175563100"}, {"first_name"=>"Jay", "last_name"=>"Unruh", "scopus_author_id"=>"7005223086"}, {"first_name"=>"Brian D.", "last_name"=>"Slaughter", "scopus_author_id"=>"6603534671"}, {"first_name"=>"Sue L.", "last_name"=>"Jaspersen", "scopus_author_id"=>"6507089398"}], "year"=>2011, "source"=>"PLoS Genetics", "identifiers"=>{"scopus"=>"2-s2.0-81755160902", "sgr"=>"81755160902", "issn"=>"15537390", "doi"=>"10.1371/journal.pgen.1002365", "pmid"=>"22125491", "isbn"=>"1553-7404 (Electronic)\\r1553-7390 (Linking)", "pui"=>"362967177"}, "id"=>"f0ae27d0-7e08-321d-a6d0-99eb3dad5343", "abstract"=>"The budding yeast spindle pole body (SPB) is anchored in the nuclear envelope so that it can simultaneously nucleate both nuclear and cytoplasmic microtubules. During SPB duplication, the newly formed SPB is inserted into the nuclear membrane. The mechanism of SPB insertion is poorly understood but likely involves the action of integral membrane proteins to mediate changes in the nuclear envelope itself, such as fusion of the inner and outer nuclear membranes. Analysis of the functional domains of the budding yeast SUN protein and SPB component Mps3 revealed that most regions are not essential for growth or SPB duplication under wild-type conditions. However, a novel dominant allele in the P-loop region, MPS3-G186K, displays defects in multiple steps in SPB duplication, including SPB insertion, indicating a previously unknown role for Mps3 in this step of SPB assembly. Characterization of the MPS3-G186K mutant by electron microscopy revealed severe over-proliferation of the inner nuclear membrane, which could be rescued by altering the characteristics of the nuclear envelope using both chemical and genetic methods. Lipid profiling revealed that cells lacking MPS3 contain abnormal amounts of certain types of polar and neutral lipids, and deletion or mutation of MPS3 can suppress growth defects associated with inhibition of sterol biosynthesis, suggesting that Mps3 directly affects lipid homeostasis. Therefore, we propose that Mps3 facilitates insertion of SPBs in the nuclear membrane by modulating nuclear envelope composition.", "link"=>"http://www.mendeley.com/research/sun-protein-mps3-required-spindle-pole-body-insertion-nuclear-membrane-nuclear-envelope-homeostasis", "reader_count"=>44, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Researcher"=>16, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>17, "Student > Postgraduate"=>1, "Other"=>1, "Student > Master"=>1, "Student > Bachelor"=>4, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Researcher"=>16, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>17, "Student > Postgraduate"=>1, "Other"=>1, "Student > Master"=>1, "Student > Bachelor"=>4, "Professor"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>2, "Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>10, "Agricultural and Biological Sciences"=>28, "Chemistry"=>1, "Psychology"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>2}, "Chemistry"=>{"Chemistry"=>1}, "Psychology"=>{"Psychology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>28}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>10}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"Belgium"=>2}, "group_count"=>1}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/361022", "https://ndownloader.figshare.com/files/361084", "https://ndownloader.figshare.com/files/361140", "https://ndownloader.figshare.com/files/361198", "https://ndownloader.figshare.com/files/361235", "https://ndownloader.figshare.com/files/361281", "https://ndownloader.figshare.com/files/361304", "https://ndownloader.figshare.com/files/361330", "https://ndownloader.figshare.com/files/361352", "https://ndownloader.figshare.com/files/361398", "https://ndownloader.figshare.com/files/361444", "https://ndownloader.figshare.com/files/361481", "https://ndownloader.figshare.com/files/361520"], "description"=>"<div><p>The budding yeast spindle pole body (SPB) is anchored in the nuclear envelope so that it can simultaneously nucleate both nuclear and cytoplasmic microtubules. During SPB duplication, the newly formed SPB is inserted into the nuclear membrane. The mechanism of SPB insertion is poorly understood but likely involves the action of integral membrane proteins to mediate changes in the nuclear envelope itself, such as fusion of the inner and outer nuclear membranes. Analysis of the functional domains of the budding yeast SUN protein and SPB component Mps3 revealed that most regions are not essential for growth or SPB duplication under wild-type conditions. However, a novel dominant allele in the P-loop region, <em>MPS3-G186K</em>, displays defects in multiple steps in SPB duplication, including SPB insertion, indicating a previously unknown role for Mps3 in this step of SPB assembly. Characterization of the <em>MPS3-G186K</em> mutant by electron microscopy revealed severe over-proliferation of the inner nuclear membrane, which could be rescued by altering the characteristics of the nuclear envelope using both chemical and genetic methods. Lipid profiling revealed that cells lacking <em>MPS3</em> contain abnormal amounts of certain types of polar and neutral lipids, and deletion or mutation of <em>MPS3</em> can suppress growth defects associated with inhibition of sterol biosynthesis, suggesting that Mps3 directly affects lipid homeostasis. Therefore, we propose that Mps3 facilitates insertion of SPBs in the nuclear membrane by modulating nuclear envelope composition.</p> </div>", "links"=>[], "tags"=>["mps3", "spindle", "insertion", "membrane", "homeostasis"], "article_id"=>131371, "categories"=>["Cell Biology", "Chemistry", "Genetics", "Microbiology"], "users"=>["Jennifer M. Friederichs", "Suman Ghosh", "Christine J. Smoyer", "Scott McCroskey", "Brandon D. Miller", "Kyle J. Weaver", "Kym M. Delventhal", "Jay Unruh", "Brian D. Slaughter", "Sue L. Jaspersen"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002365.s001", "https://dx.doi.org/10.1371/journal.pgen.1002365.s002", "https://dx.doi.org/10.1371/journal.pgen.1002365.s003", "https://dx.doi.org/10.1371/journal.pgen.1002365.s004", "https://dx.doi.org/10.1371/journal.pgen.1002365.s005", "https://dx.doi.org/10.1371/journal.pgen.1002365.s006", "https://dx.doi.org/10.1371/journal.pgen.1002365.s007", "https://dx.doi.org/10.1371/journal.pgen.1002365.s008", "https://dx.doi.org/10.1371/journal.pgen.1002365.s009", "https://dx.doi.org/10.1371/journal.pgen.1002365.s010", "https://dx.doi.org/10.1371/journal.pgen.1002365.s011", "https://dx.doi.org/10.1371/journal.pgen.1002365.s012", "https://dx.doi.org/10.1371/journal.pgen.1002365.s013"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/The_SUN_Protein_Mps3_Is_Required_for_Spindle_Pole_Body_Insertion_into_the_Nuclear_Membrane_and_Nuclear_Envelope_Homeostasis/131371", "title"=>"The SUN Protein Mps3 Is Required for Spindle Pole Body Insertion into the Nuclear Membrane and Nuclear Envelope Homeostasis", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2011-11-17 00:22:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/710272"], "description"=>"<p>(A) Schematic of Mps3 showing the N-terminal acidic region, transmembrane domain (TM), P-loop motif, coiled-coils (cc), poly-glutamine (pQ) repeat and C-terminal SUN domain. Alignment of P-loop consensus sequence with amino acids 186–194 of Mps3 is shown below. (B) The indicated mutants within each Mps3 domain were tested for their ability to rescue a deletion of <i>MPS3</i> (SLJ2039). + and − indicate growth and death, respectively, of mutants on 5-fluoroorotic acid (5-FOA) at 30°C. Viable alleles were analyzed further to determine DNA content by flow cytometry and tested for genetic interactions with other SPB mutants, including <i>kar1Δ17</i> (SLJ844), <i>cdc31-2</i> (SLJ894), <i>mps2-1</i> (SLJ718), <i>sfi1-3</i> (SLJ1558) and <i>CDC31-16</i> (SLJ907). Alleles were also fused to GFP to determine the subcellular distribution of the mutant protein using confocal imaging <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002365#pgen.1002365-Bupp1\" target=\"_blank\">[41]</a>. *Non-functional mutants were localized in the presence of wild-type Mps3. ND, not determined. **The <i>mps3-S190A</i> is dependent of copy number: single copy integrants are viable while multiple copy integrants are lethal, as shown in the serial dilution assay in (D). (C) A representative single plane confocal image showing the localization of Mps3-GFP (SLJ2571) and mps3ΔTM-GFP (SLJ4308) (green) together with H2B-mCherry (red) in cells grown at 23°C. Bar, 2 µm. The covering plasmid was removed from SLJ2571 by plating cells to 2-amino-5-fluorobenzoic acid (5-FBA) plates immediately prior to use; because mps3ΔTM-GFP is non-functional, the wild-type untagged copy of <i>MPS3</i> could not be lost. (D) Serial dilution assay of SLJ2039 containing no insert (-), wild-type <i>MPS3</i>, or <i>mps3-S190A</i> integrated in 1 copy or 2 copies on SD-URA and 5-FOA, which removes the covering plasmid containing wild-type <i>MPS3</i>. Plates were grown for 2 d at 30°C. (E) Wild-type (SLJ001), <i>GAL-MPS3</i> (SLJ995) and <i>GAL-MPS3-G186K</i> (SLJ1797) cells were tested for their ability to grow on YPD (glucose) and YPGR (galactose) plates at 30°C in a serial dilution assay.</p>", "links"=>[], "tags"=>["regions", "mps3", "spb"], "article_id"=>380625, "categories"=>["Cell Biology", "Chemistry", "Genetics", "Microbiology"], "users"=>["Jennifer M. Friederichs", "Suman Ghosh", "Christine J. Smoyer", "Scott McCroskey", "Brandon D. Miller", "Kyle J. Weaver", "Kym M. Delventhal", "Jay Unruh", "Brian D. Slaughter", "Sue L. Jaspersen"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002365.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Essential_regions_of_Mps3_for_SPB_duplication_/380625", "title"=>"Essential regions of Mps3 for SPB duplication.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-11-17 00:10:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/710414"], "description"=>"<p>(A,B) <i>GAL-MPS3</i> (SLJ995) and <i>GAL-MPS3-G186K</i> (SLJ1797) cells were grown overnight at 30°C in YEP plus 2% raffinose then 2% galactose was added for 5 h to induce expression. (A) DNA content was analyzed by flow cytometry and budding index was determined using phase contrast microscopy. The percentage of large-budded cells is indicated (n = 300). (B) In addition, spindle morphology was examined using indirect immunofluorescence microscopy using anti-α-tubulin (green), anti-γ-tubulin (red) and DAPI (blue). The percentage of large budded cells (n>200) with bipolar and both types of monopolar spindles is indicated for each strain. Bar, 5 µm. (C) Schematic of SPB duplication pathway showing the timing of Spc110-GFP (green) and Spc42-mCherry (red) assembly. (D) SPBs were visualized using Spc110-GFP (green) and Spc42-mCherry (red) in <i>GAL-MPS3</i> (SLJ4859) and <i>GAL-MPS3-G186K</i> (SLJ4864) cells grown for 5 h in SC-HIS+2% galactose+2% raffinose at 30°C. Arrows point to unduplicated SPBs that contain only Spc42-mCherry, and an asterisk marks a SPB fragment seen in <i>MPS3-G186K</i>, which contains only Spc110-GFP. This may represent a delaminated SPB similar to that observed in some <i>spc110</i> mutants <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002365#pgen.1002365-Yoder1\" target=\"_blank\">[101]</a>. Bar, 5 µm. (E) The number of SPBs containing the indicated number of Spc42-mCherry and Spc110-GFP foci in large budded cells was quantitated in two independent experiments (n>250). We also counted SPB foci in SLJ4859 cells grown in SC-HIS+2% raffinose+2% glucose for 5 h at 30°C. (F–H) <i>GAL-MPS3-G186K</i> (SLJ1797) mutants were also processed for thin-section EM to examine SPB morphology following induction for 5 h in YPGR at 30°C. Serial sections through nuclei of 15 cells were collected: (F) 7 nuclei contained a single unduplicated SPB, (G) 4 nuclei contained an SPB as well as electron dense material (arrow) resembling a SPB precursor on the cytoplasmic face of the nuclear envelope, and (H) 4 nuclei contained duplicated and separated SPBs, which nucleated a short bipolar spindle. The inset in (G) shows an adjacent section with a cytoplasmic microtubule emanating from the SPB precursor (position marked with an arrow). (F,G) Bar, 100 nm. (H) Bar, 200 nm.</p>", "links"=>[], "tags"=>["causes", "mitotic", "spb", "duplication"], "article_id"=>380768, "categories"=>["Cell Biology", "Chemistry", "Genetics", "Microbiology"], "users"=>["Jennifer M. Friederichs", "Suman Ghosh", "Christine J. Smoyer", "Scott McCroskey", "Brandon D. Miller", "Kyle J. Weaver", "Kym M. Delventhal", "Jay Unruh", "Brian D. Slaughter", "Sue L. Jaspersen"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002365.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_MPS3_G186K_expression_causes_a_mitotic_arrest_due_to_SPB_duplication_defects_/380768", "title"=>"<i>MPS3-G186K</i> expression causes a mitotic arrest due to SPB duplication defects.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-11-17 00:12:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/710574"], "description"=>"<p><i>GAL-MPS3</i> (SLJ995) and <i>GAL-MPS3-G186K</i> (SLJ1797) cells were processed for thin-section EM following induction for 5 h in YPGR at 30°C. (A) Nuclei from <i>GAL-MPS3</i> cells show a round or elliptical morphology (50%; n = 17) similar to that seen in wild-type cells (see <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002365#pgen.1002365-Winey3\" target=\"_blank\">[55]</a>), or (B) an abnormal nuclear morphology (50%; n = 17). In all nuclei from <i>GAL-MPS3</i> cells, only a single nuclear envelope bi-layer was observed. (C–F) Nuclei from <i>GAL-MPS3-G186K</i> all (n = 50) exhibited an abnormal nuclear morphology, which included multiple lobes (C), as well as highly curved projections (D). Multiple nuclear envelope layers were present, and this often resulted in (E) clustered NPCs in regions containing a single nuclear membrane layer. In other cases (F), stacked NPCs were observed. (A–F) Bar, 200 nm. (G) <i>GAL-MPS3</i> (SLJ5097) and <i>GAL-MPS3-G186K</i> (SLJ5098) cells containing Nup49-GFP and Net1-mCherry were grown to mid-log phase in YEP+2% raffinose, then 2% glucose was added to half and 2% galactose was added to the other half and cells were allowed to grow for 5 h at 30°C. Mislocalization of Nup49-GFP (green) and Net1-mCherry (red) in <i>MPS3-G186K</i> in galactose are consistent with aberrant nuclear morphology seen by EM, but no NPCs were observed in the cytoplasm in either mutant. Bar, 5 µm.</p>", "links"=>[], "tags"=>["membrane"], "article_id"=>380929, "categories"=>["Cell Biology", "Chemistry", "Genetics", "Microbiology"], "users"=>["Jennifer M. Friederichs", "Suman Ghosh", "Christine J. Smoyer", "Scott McCroskey", "Brandon D. Miller", "Kyle J. Weaver", "Kym M. Delventhal", "Jay Unruh", "Brian D. Slaughter", "Sue L. Jaspersen"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002365.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Over_proliferation_of_the_nuclear_membrane_in_GAL_MPS3_G186K_/380929", "title"=>"Over-proliferation of the nuclear membrane in <i>GAL-MPS3-G186K</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-11-17 00:15:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/710709"], "description"=>"<p>EM images from <i>GAL-MPS3-G186K</i> cells grown for 5 h in YPGR at 30°C showing the formation of intranuclear tubules. (A) In the cell on the left, the positions of tubule formation are indicated by a single or double asterisk, and higher magnification images of these regions are shown on the right. In the center image, the outer layers of the nuclear membrane appear to have extended into the cytoplasm to enclose a region of the cytosol. An arrow points to a tubule forming what appears to be a third nuclear layer in the right image. (B) The cell shown has at least three interesting regions of membrane expansion, which are denoted and shown in higher magnification. In region 1, at least two lipid bilayers have encircled a region of the cytosol. In region 2, expansion of membranes within the nucleus is not uniform. Here, membrane proliferation is occurring inside the nucleus to compartmentalize the nucleolus away from rest of the nucleus. In region 3, membrane is also encircling a cytoplasmic region, but it is clear that the membranes are folding back on themselves to form a tubule inside the membrane bilayers. Bars, 200 nm.</p>", "links"=>[], "tags"=>["tubules", "intranuclear", "membranes"], "article_id"=>381059, "categories"=>["Cell Biology", "Chemistry", "Genetics", "Microbiology"], "users"=>["Jennifer M. Friederichs", "Suman Ghosh", "Christine J. Smoyer", "Scott McCroskey", "Brandon D. Miller", "Kyle J. Weaver", "Kym M. Delventhal", "Jay Unruh", "Brian D. Slaughter", "Sue L. Jaspersen"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002365.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Formation_of_tubules_and_intranuclear_membranes_in_MPS3_G186K_cells_/381059", "title"=>"Formation of tubules and intranuclear membranes in <i>MPS3-G186K</i> cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-11-17 00:17:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/710889"], "description"=>"<p>(A) Wild-type (SLJ001), <i>GAL-MPS3</i> (SLJ995) and <i>GAL-MPS3-G186K</i> (SLJ1797) cells were tested for their ability to grow on YPGR, YPGR+0.2% benzyl alcohol, YPGR+5 mM oleic acid and YPGR+2.5 µg/ml terbinafine plates at 30°C in a serial dilution assay. Plates were grown for 2 d. Cells were also stamped to YPGR at 16°C for 7 d, 23°C for 3 d and 33°C for 2 d to test the effect of temperature on colony formation. (B) <i>GAL-MPS3</i> (SLJ4963) and <i>GAL-MPS3-G186K</i> (SLJ4965) containing HDEL-dsRed (red) and Pus1-GFP (green) were grown overnight in SC-LEU+2% raffinose then 2% glucose, 2% galactose, 2% galactose+5 mM oleic acid, 2% galactose+0.2% benzyl alcohol, or 2% galactose+1.25 µg/ml terbinafine was added for 6 h at 30°C to visualize the effects on nuclear morphology. Bar, 5 µm. (C–D) <i>GAL-MPS3</i> (SLJ4859) and <i>GAL-MPS3-G186K</i> (SLJ4864) cells were grown in SC-HIS+2% raffinose, then treated as in (B). A sample containing 2% galactose was also shifted to 33°C for 6 h. (C) DNA content was analyzed by flow cytometry. (D) In these same cells, the number of SPBs containing the indicated number of Spc42-mCherry and Spc110-GFP foci in large budded cells was quantitated (n>100 for all samples).</p>", "links"=>[], "tags"=>["membrane", "properties", "suppresses"], "article_id"=>381243, "categories"=>["Cell Biology", "Chemistry", "Genetics", "Microbiology"], "users"=>["Jennifer M. Friederichs", "Suman Ghosh", "Christine J. Smoyer", "Scott McCroskey", "Brandon D. Miller", "Kyle J. Weaver", "Kym M. Delventhal", "Jay Unruh", "Brian D. Slaughter", "Sue L. Jaspersen"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002365.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Alteration_of_membrane_properties_suppresses_MPS3_G186K_/381243", "title"=>"Alteration of membrane properties suppresses <i>MPS3-G186K</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-11-17 00:20:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/711015"], "description"=>"<p>(A) Mutants from the yeast deletion collection were transformed with p<i>URA3-GAL-MPS3-G186K</i>, and transformants were tested for their ability to grow on SC-URA+2% galactose at for 3 d at 30°C. Possible hits were then further examined by western blotting to ensure that Mps3-G186K was expressed at high levels, similar to the wild-type control. Shown here are the hits that both rescued the growth defect of <i>MPS3-G186K</i> and expressed wild-type levels of Mps3-G186K. A full list of genes recovered in this screen is presented in <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002365#pgen.1002365.s007\" target=\"_blank\">Table S2</a>. Also shown are the wild-type strain and the <i>gal4Δ</i> mutant, which we isolated in the primary screen but eliminated in the secondary screen because it does not express <i>MPS3-G186K</i>. Hits are organized by function using GoSlim analysis. In some cases, we isolated a mutant that deletes part of an overlapping gene, which is indicated below in parenthesis. (B) The indicated genes were deleted in <i>GAL-MPS3-G186K</i> (SLJ1797) and growth was tested in a serial dilution assay at 30°C on YPD (glucose) for 2 d and YPGR (galactose) for 3 d.</p>", "links"=>[], "tags"=>["genetics and genomics", "chemistry", "microbiology", "cell biology"], "article_id"=>381370, "categories"=>["Cell Biology", "Chemistry", "Genetics", "Microbiology"], "users"=>["Jennifer M. Friederichs", "Suman Ghosh", "Christine J. Smoyer", "Scott McCroskey", "Brandon D. Miller", "Kyle J. Weaver", "Kym M. Delventhal", "Jay Unruh", "Brian D. Slaughter", "Sue L. Jaspersen"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002365.g006"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Suppressors_of_MPS3_G186K_toxicity_/381370", "title"=>"Suppressors of <i>MPS3-G186K</i> toxicity.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-11-17 00:22:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/711146"], "description"=>"<p>(A) Wild-type (SLJ1779), <i>mps-Q572LQ573L</i> (SLJ1783), <i>mps3-W487A</i> (SLJ1785), <i>mps3-W477A</i> (SLJ1793), <i>mps3ΔSUN2</i> (SLJ1789), <i>mps3-A540D</i> (SLJ1787), <i>mps3-Y502H</i> (SLJ1781) and <i>mps3-F592S</i> (SLJ1791) cells, or (B) wild-type (SLJ001), <i>mps3-1</i> (SLJ910), <i>cdc31-2</i> (SLJ894), <i>kar1Δ17</i> (SLJ844), <i>sfi1-3</i> (SLJ1558), <i>mps2-1</i> (SLJ718), <i>spc42-11</i> (SLJ715) and <i>ndc1-39</i> (SLJ1740) cells, were grown to log-phase in YPD, then were tested for their ability to grown on YPD, YPD+0.2% benzyl alcohol and YPD+2.5 µg/ml terbinafine plates in a serial dilution assay at 23°C for 3 d. In (B) cells were also grown on YPD+5 mM oleic acid.</p>", "links"=>[], "tags"=>["mutants", "changes", "membrane"], "article_id"=>381495, "categories"=>["Cell Biology", "Chemistry", "Genetics", "Microbiology"], "users"=>["Jennifer M. Friederichs", "Suman Ghosh", "Christine J. Smoyer", "Scott McCroskey", "Brandon D. Miller", "Kyle J. Weaver", "Kym M. Delventhal", "Jay Unruh", "Brian D. Slaughter", "Sue L. Jaspersen"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002365.g007"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_mps3_mutants_are_sensitive_to_changes_in_membrane_properties_/381495", "title"=>"<i>mps3</i> mutants are sensitive to changes in membrane properties.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-11-17 00:24:55"}
  • {"files"=>["https://ndownloader.figshare.com/files/711370"], "description"=>"<p>Lipids were extracted from 5 independently grown mid-log phase cultures at 30°C of wild-type (SLJ001), <i>pom152Δ</i> (SLJ4260) and <i>pom152Δ mps3Δ</i> (SLJ4259) and analyzed by ESI/MS/MS. The total amount of each type of lipid was calculated per mg weight of wet yeast cells used, and average values for each class of lipid were tabulated along with the standard deviation from the mean. A complete summary of our lipidomics analysis is presented in <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002365#pgen.1002365.s008\" target=\"_blank\">Table S3</a>. (A) Although there is no change in the levels of total polar lipids, different types of phospholipids are increased or decreased in <i>pom152Δ mps3Δ</i> (white) compared to wild-type (black) or <i>pom152Δ</i> (gray). <i>pom152Δ mps3Δ</i> cells also show increased amounts of TAG. (B) Cells lacking <i>POM152</i> only contain decreased levels of ergosterols and DAGs. Deletion of <i>MPS3</i> in these cells restores the level of ergosterols and DAGs to that observed in wild-type cells. (A & B) Black asterisks indicate values that are statistically significant from wild-type and red asterisks indicate values that are statistically significant from <i>pom152Δ</i> (p<0.05).</p>", "links"=>[], "tags"=>["lipid", "levels", "cells", "lacking", "mps3"], "article_id"=>381714, "categories"=>["Cell Biology", "Chemistry", "Genetics", "Microbiology"], "users"=>["Jennifer M. Friederichs", "Suman Ghosh", "Christine J. Smoyer", "Scott McCroskey", "Brandon D. Miller", "Kyle J. Weaver", "Kym M. Delventhal", "Jay Unruh", "Brian D. Slaughter", "Sue L. Jaspersen"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002365.g008"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Aberrant_lipid_levels_in_cells_lacking_Mps3_function_/381714", "title"=>"Aberrant lipid levels in cells lacking Mps3 function.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-11-17 00:28:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/711466"], "description"=>"<p>(A) Wild-type (SLJ001), <i>pom152Δ</i> (SLJ4260) and <i>pom152Δ mps3Δ</i> (SLJ5247) cells were tested for their ability to grow on YPD and YPD+0.2% benzyl alcohol at 23°C and 37°C in a serial dilution assay. Plates were grown for 2 d at 37°C and for 4 d at 23°C. (B) These same cells were transformed with an empty plasmid or with <i>GAL-FAA3</i> and tested for their ability to grow on YPGR and YPGR+0.2% benzyl alcohol at 37°C for 2 d in a serial dilution assay.</p>", "links"=>[], "tags"=>["defects", "cells", "lacking", "suppressed"], "article_id"=>381816, "categories"=>["Cell Biology", "Chemistry", "Genetics", "Microbiology"], "users"=>["Jennifer M. Friederichs", "Suman Ghosh", "Christine J. Smoyer", "Scott McCroskey", "Brandon D. Miller", "Kyle J. Weaver", "Kym M. Delventhal", "Jay Unruh", "Brian D. Slaughter", "Sue L. Jaspersen"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002365.g009"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Membrane_defects_in_cells_lacking_MPS3_can_be_suppressed_by_FAA3_/381816", "title"=>"Membrane defects in cells lacking <i>MPS3</i> can be suppressed by <i>FAA3</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-11-17 00:30:16"}

PMC Usage Stats | Further Information

  • {"month"=>"11", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"1", "year"=>"2011", "pdf"=>"0", "unique-ip"=>"1", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"12", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"48", "unique-ip"=>"42", "pdf"=>"23", "year"=>"2011", "figure"=>"22", "scanned-summary"=>"0", "supp-data"=>"15"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"36", "unique-ip"=>"30", "pdf"=>"17", "year"=>"2012", "figure"=>"23", "scanned-summary"=>"0", "supp-data"=>"24"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"16", "year"=>"2012", "pdf"=>"7", "unique-ip"=>"16", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"3"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"12", "unique-ip"=>"15", "pdf"=>"4", "year"=>"2012", "figure"=>"14", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"18", "year"=>"2012", "pdf"=>"14", "unique-ip"=>"23", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"22", "unique-ip"=>"15", "pdf"=>"8", "year"=>"2012", "figure"=>"9", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"21", "year"=>"2012", "pdf"=>"15", "unique-ip"=>"20", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"16"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"15", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"17", "full-text"=>"15", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"21", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"38", "full-text"=>"27", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"16", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"36", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"14", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"2", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"7", "pdf"=>"0", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"3", "cited-by"=>"1", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"19", "full-text"=>"39", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"29", "full-text"=>"229", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"4", "cited-by"=>"1", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"30", "full-text"=>"154", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"27", "full-text"=>"145", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"56", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"22", "full-text"=>"17", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"21", "full-text"=>"14", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"16", "cited-by"=>"1", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"20", "full-text"=>"21", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"18", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"2", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"15", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"15", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"3", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"0", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"2", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"21", "full-text"=>"21", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"8", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"2", "full-text"=>"0", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2011-01-01T00:00:00Z", "end_date"=>"2011-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[290, 559, 698, 813, 927, 1028, 1121, 1206, 1289, 1360, 1433, 1501, 1569, 1637, 1707, 1774, 1841, 1911, 1974, 2038, 2103, 2161, 2219, 2280, 2345, 2406, 2466, 2529, 2592, 2654, 2713, 2772, 2835, 2893, 2954, 3020, 3071]}, {"subject_area"=>"/Physical sciences/Chemistry", "average_usage"=>[272, 506, 626, 733, 837, 944, 1031, 1116, 1192, 1253, 1324, 1388, 1459, 1528, 1593, 1653, 1718, 1787, 1854, 1916, 1974, 2045, 2111, 2167, 2232, 2297, 2355, 2425, 2489, 2549, 2615, 2675, 2734, 2793, 2851, 2904, 2961]}]}
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