The Dynamics and Prognostic Potential of DNA Methylation Changes at Stem Cell Gene Loci in Women's Cancer
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"The dynamics and prognostic potential of DNA methylation changes at stem cell gene loci in women's Cancer", "type"=>"journal", "authors"=>[{"first_name"=>"Joanna", "last_name"=>"Zhuang", "scopus_author_id"=>"54379435300"}, {"first_name"=>"Allison", "last_name"=>"Jones", "scopus_author_id"=>"7407102219"}, {"first_name"=>"Shih Han", "last_name"=>"Lee", "scopus_author_id"=>"55156775300"}, {"first_name"=>"Esther", "last_name"=>"Ng", "scopus_author_id"=>"24169653900"}, {"first_name"=>"Heidi", "last_name"=>"Fiegl", "scopus_author_id"=>"6602633455"}, {"first_name"=>"Michal", "last_name"=>"Zikan", "scopus_author_id"=>"6507167275"}, {"first_name"=>"David", "last_name"=>"Cibula", "scopus_author_id"=>"7006225985"}, {"first_name"=>"Alexandra", "last_name"=>"Sargent", "scopus_author_id"=>"14027368000"}, {"first_name"=>"Helga B.", "last_name"=>"Salvesen", "scopus_author_id"=>"7004474802"}, {"first_name"=>"Ian J.", "last_name"=>"Jacobs", "scopus_author_id"=>"34975076100"}, {"first_name"=>"Henry C.", "last_name"=>"Kitchener", "scopus_author_id"=>"35451402200"}, {"first_name"=>"Andrew E.", "last_name"=>"Teschendorff", "scopus_author_id"=>"8528968400"}, {"first_name"=>"Martin", "last_name"=>"Widschwendter", "scopus_author_id"=>"7006520678"}], "year"=>2012, "source"=>"PLoS Genetics", "identifiers"=>{"pmid"=>"22346766", "sgr"=>"84859166725", "doi"=>"10.1371/journal.pgen.1002517", "scopus"=>"2-s2.0-84859166725", "pui"=>"364547164", "isbn"=>"1553-7404 (Electronic)\\r1553-7390 (Linking)", "issn"=>"15537390"}, "id"=>"1c785a74-2702-3e33-99d4-62d5596f1fa5", "abstract"=>"Aberrant DNA methylation is an important cancer hallmark, yet the dynamics of DNA methylation changes in human carcinogenesis remain largely unexplored. Moreover, the role of DNA methylation for prediction of clinical outcome is still uncertain and confined to specific cancers. Here we perform the most comprehensive study of DNA methylation changes throughout human carcinogenesis, analysing 27,578 CpGs in each of 1,475 samples, ranging from normal cells in advance of non-invasive neoplastic transformation to non-invasive and invasive cancers and metastatic tissue. We demonstrate that hypermethylation at stem cell PolyComb Group Target genes (PCGTs) occurs in cytologically normal cells three years in advance of the first morphological neoplastic changes, while hypomethylation occurs preferentially at CpGs which are heavily Methylated in Embryonic Stem Cells (MESCs) and increases significantly with cancer invasion in both the epithelial and stromal tumour compartments. In contrast to PCGT hypermethylation, MESC hypomethylation progresses significantly from primary to metastatic cancer and defines a poor prognostic signature in four different gynaecological cancers. Finally, we associate expression of TET enzymes, which are involved in active DNA demethylation, to MESC hypomethylation in cancer. These findings have major implications for cancer and embryonic stem cell biology and establish the importance of systemic DNA hypomethylation for predicting prognosis in a wide range of different cancers.", "link"=>"http://www.mendeley.com/research/dynamics-prognostic-potential-dna-methylation-changes-stem-cell-gene-loci-womens-cancer", "reader_count"=>82, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>7, "Researcher"=>24, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>25, "Student > Postgraduate"=>1, "Student > Master"=>11, "Other"=>2, "Student > Bachelor"=>4, "Professor"=>5}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>7, "Researcher"=>24, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>25, "Student > Postgraduate"=>1, "Student > Master"=>11, "Other"=>2, "Student > Bachelor"=>4, "Professor"=>5}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>12, "Nursing and Health Professions"=>1, "Mathematics"=>4, "Agricultural and Biological Sciences"=>34, "Medicine and Dentistry"=>18, "Neuroscience"=>2, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Chemistry"=>2, "Social Sciences"=>2, "Computer Science"=>2}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>18}, "Neuroscience"=>{"Neuroscience"=>2}, "Chemistry"=>{"Chemistry"=>2}, "Social Sciences"=>{"Social Sciences"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>34}, "Computer Science"=>{"Computer Science"=>2}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>12}, "Mathematics"=>{"Mathematics"=>4}, "Unspecified"=>{"Unspecified"=>3}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"Austria"=>1, "United States"=>4, "Japan"=>2, "Denmark"=>2, "Brazil"=>1, "Germany"=>1}, "group_count"=>5}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84859166725"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84859166725?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84859166725&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84859166725&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84859166725", "dc:identifier"=>"SCOPUS_ID:84859166725", "eid"=>"2-s2.0-84859166725", "dc:title"=>"The dynamics and prognostic potential of DNA methylation changes at stem cell gene loci in women's Cancer", "dc:creator"=>"Zhuang J.", "prism:publicationName"=>"PLoS Genetics", "prism:issn"=>"15537390", "prism:eIssn"=>"15537404", "prism:volume"=>"8", "prism:issueIdentifier"=>"2", "prism:pageRange"=>nil, "prism:coverDate"=>"2012-02-01", "prism:coverDisplayDate"=>"February 2012", "prism:doi"=>"10.1371/journal.pgen.1002517", "citedby-count"=>"81", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"University College London", "affiliation-city"=>"London", "affiliation-country"=>"United Kingdom"}], "pubmed-id"=>"22346766", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e1002517", "source-id"=>"4000151808", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosgenetics%2Farticle%3Fid%3D10.1371%252Fjournal.pgen.1002517", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"227", "xml_views"=>"12", "html_views"=>"987"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"106", "xml_views"=>"31", "html_views"=>"514"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"76", "xml_views"=>"1", "html_views"=>"218"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"65", "xml_views"=>"1", "html_views"=>"150"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"31", "xml_views"=>"4", "html_views"=>"105"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"39", "xml_views"=>"0", "html_views"=>"119"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"48", "xml_views"=>"6", "html_views"=>"173"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"49", "xml_views"=>"2", "html_views"=>"221"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"45", "xml_views"=>"1", "html_views"=>"201"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"37", "xml_views"=>"0", "html_views"=>"126"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"41", "xml_views"=>"0", "html_views"=>"136"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"30", "xml_views"=>"3", "html_views"=>"125"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"24", "xml_views"=>"2", "html_views"=>"251"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"106"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"105"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"95"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"20", "xml_views"=>"2", "html_views"=>"108"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"105"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"79"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"83"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"339"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"201"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"128"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"19", "xml_views"=>"1", "html_views"=>"90"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"101"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"92"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"117"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"95"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"92"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"18", "xml_views"=>"2", "html_views"=>"70"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"72"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"94"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"14", "xml_views"=>"2", "html_views"=>"91"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"40"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"43"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"68"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"13"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"1"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"3", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"6"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"15"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"10", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"1"}
  • {"month"=>"11", "year"=>"2020", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"8"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/683645"], "description"=>"<p>Scatter plots of mean β-values in normal cervical tissue (x-axes) vs. cervical cancer tissue (y-axes) of (A) all CpGs with PCGTs highlighted in brown and (B) all CpGs with MESCs highlighted in purple. (C) is the bar chart indicating the enrichment odds ratios (OR) and P-values (Fisher-test), testing for enrichment of PCGTs among CpGs unmethylated in normal cervix (mean β-value<0.2) and with a higher mean β-value in (i) normal samples which develop dysplasia (BDy), (ii) non-invasive dysplastic samples (Dy), and (iii) invasive cervical cancer (CA); (D) the bar chart indicating the enrichment of MESCs among those CpGs methylated in the normal cervix (mean β-value>0.4) and with a lower mean β-value in tissue representing the three stages of cervical carcinogenesis (BDy, Dy, and CA).</p>", "links"=>[], "tags"=>["pcgts", "mescs", "cervix"], "article_id"=>354112, "categories"=>["Genetics"], "users"=>["Joanna Zhuang", "Allison Jones", "Shih-Han Lee", "Esther Ng", "Heidi Fiegl", "Michal Zikan", "David Cibula", "Alexandra Sargent", "Helga B. Salvesen", "Ian J. Jacobs", "Henry C. Kitchener", "Andrew E. Teschendorff", "Martin Widschwendter"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002517.g001", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Methylation_profile_of_PCGTs_and_MESCs_in_cervix_data_/354112", "title"=>"Methylation profile of PCGTs and MESCs in cervix data.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-02-09 01:08:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/684019"], "description"=>"<p>Kaplan-Meier survival curves between the upper (blue) and lower (green) tertiles of the demethylation instability index (DeMI) at MESCs in (A) Endometrial cancer, (B) Ovarian cancer, (C) Cervical cancer, and (D–E) two Breast Cancer cohorts. The hazard ratio (HR), 95% confidence interval (CI) and P-values are from the multivariate Cox regression model, with “n” denoting the number of samples in cohort. Clinical endpoint used is indicated on the y-axis (OS = overall survival, RFS = relapse free survival).</p>", "links"=>[], "tags"=>["mescs", "demethylation", "instability"], "article_id"=>354497, "categories"=>["Genetics"], "users"=>["Joanna Zhuang", "Allison Jones", "Shih-Han Lee", "Esther Ng", "Heidi Fiegl", "Michal Zikan", "David Cibula", "Alexandra Sargent", "Helga B. Salvesen", "Ian J. Jacobs", "Henry C. Kitchener", "Andrew E. Teschendorff", "Martin Widschwendter"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002517.g004", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Survival_analysis_of_the_MESCs_demethylation_instability_index_in_various_cancers_/354497", "title"=>"Survival analysis of the MESCs demethylation instability index in various cancers.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-02-09 01:14:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/684571"], "description"=>"<p>Cervical cells collected three years before half of the patients developed dysplasia (BDy), normal versus dysplastic cervical cells (Dy), normal cervical tissue and invasive cervical cancer (Cvx CA), non-neoplastic breast tissue and breast cancer (BC), normal endometrium and endometrial cancer (EC) and metastatic endometrial cancer (EC (Meta)), ovarian cancer tissue (OvC; The Cancer Genome Atlas, TCGA), colon cancer tissue (ColC), lung cancer tissue (LC) and normal and cancer associated lung fibroblasts (LC Fibro), and white blood cell (WBC) samples from <i>BRCA1</i> mutation carriers and controls (<i>BRCA1</i>). The number of samples, their distribution in terms of normal and cancer, cell-type, age-range (years) and reference to data access is given. GSE numbers are GEO accessions. NA, not available.</p>", "links"=>[], "tags"=>["sets"], "article_id"=>355047, "categories"=>["Genetics"], "users"=>["Joanna Zhuang", "Allison Jones", "Shih-Han Lee", "Esther Ng", "Heidi Fiegl", "Michal Zikan", "David Cibula", "Alexandra Sargent", "Helga B. Salvesen", "Ian J. Jacobs", "Henry C. Kitchener", "Andrew E. Teschendorff", "Martin Widschwendter"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002517.t001", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Data_sets_used_in_this_study_/355047", "title"=>"Data sets used in this study.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-02-09 01:24:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/349007", "https://ndownloader.figshare.com/files/349034", "https://ndownloader.figshare.com/files/349074", "https://ndownloader.figshare.com/files/349107", "https://ndownloader.figshare.com/files/349147", "https://ndownloader.figshare.com/files/349288", "https://ndownloader.figshare.com/files/349345", "https://ndownloader.figshare.com/files/349394", "https://ndownloader.figshare.com/files/349436", "https://ndownloader.figshare.com/files/349475", "https://ndownloader.figshare.com/files/349498", "https://ndownloader.figshare.com/files/349509", "https://ndownloader.figshare.com/files/349526", "https://ndownloader.figshare.com/files/349543", "https://ndownloader.figshare.com/files/349552", "https://ndownloader.figshare.com/files/349566", "https://ndownloader.figshare.com/files/349582", "https://ndownloader.figshare.com/files/349595"], "description"=>"<div><p>Aberrant DNA methylation is an important cancer hallmark, yet the dynamics of DNA methylation changes in human carcinogenesis remain largely unexplored. Moreover, the role of DNA methylation for prediction of clinical outcome is still uncertain and confined to specific cancers. Here we perform the most comprehensive study of DNA methylation changes throughout human carcinogenesis, analysing 27,578 CpGs in each of 1,475 samples, ranging from normal cells in advance of non-invasive neoplastic transformation to non-invasive and invasive cancers and metastatic tissue. We demonstrate that hypermethylation at stem cell PolyComb Group Target genes (PCGTs) occurs in cytologically normal cells three years in advance of the first morphological neoplastic changes, while hypomethylation occurs preferentially at CpGs which are heavily Methylated in Embryonic Stem Cells (MESCs) and increases significantly with cancer invasion in both the epithelial and stromal tumour compartments. In contrast to PCGT hypermethylation, MESC hypomethylation progresses significantly from primary to metastatic cancer and defines a poor prognostic signature in four different gynaecological cancers. Finally, we associate expression of TET enzymes, which are involved in active DNA demethylation, to MESC hypomethylation in cancer. These findings have major implications for cancer and embryonic stem cell biology and establish the importance of systemic DNA hypomethylation for predicting prognosis in a wide range of different cancers.</p> </div>", "links"=>[], "tags"=>["prognostic", "dna", "methylation", "changes", "loci", "cancer"], "article_id"=>129003, "categories"=>["Genetics"], "users"=>["Joanna Zhuang", "Allison Jones", "Shih-Han Lee", "Esther Ng", "Heidi Fiegl", "Michal Zikan", "David Cibula", "Alexandra Sargent", "Helga B. Salvesen", "Ian J. Jacobs", "Henry C. Kitchener", "Andrew E. Teschendorff", "Martin Widschwendter"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002517.s001", "https://dx.doi.org/10.1371/journal.pgen.1002517.s002", "https://dx.doi.org/10.1371/journal.pgen.1002517.s003", "https://dx.doi.org/10.1371/journal.pgen.1002517.s004", "https://dx.doi.org/10.1371/journal.pgen.1002517.s005", "https://dx.doi.org/10.1371/journal.pgen.1002517.s006", "https://dx.doi.org/10.1371/journal.pgen.1002517.s007", "https://dx.doi.org/10.1371/journal.pgen.1002517.s008", "https://dx.doi.org/10.1371/journal.pgen.1002517.s009", "https://dx.doi.org/10.1371/journal.pgen.1002517.s010", "https://dx.doi.org/10.1371/journal.pgen.1002517.s011", "https://dx.doi.org/10.1371/journal.pgen.1002517.s012", "https://dx.doi.org/10.1371/journal.pgen.1002517.s013", "https://dx.doi.org/10.1371/journal.pgen.1002517.s014", "https://dx.doi.org/10.1371/journal.pgen.1002517.s015", "https://dx.doi.org/10.1371/journal.pgen.1002517.s016", "https://dx.doi.org/10.1371/journal.pgen.1002517.s017", "https://dx.doi.org/10.1371/journal.pgen.1002517.s018"], "stats"=>{"downloads"=>41, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/The_Dynamics_and_Prognostic_Potential_of_DNA_Methylation_Changes_at_Stem_Cell_Gene_Loci_in_Women_s_Cancer/129003", "title"=>"The Dynamics and Prognostic Potential of DNA Methylation Changes at Stem Cell Gene Loci in Women's Cancer", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-02-09 02:30:03"}
  • {"files"=>["https://ndownloader.figshare.com/files/684289"], "description"=>"<p>The CpGs show stable fully methylated states across all normal samples. Methylation values: blue = high methylation (β-value>0.7), skyblue = hemi methylation (0.25<β-value<0.7), yellow = low methylation (β-value<0.25). Sample labels at the top of the heatmaps: normal (grey) and cancer (black).</p>", "links"=>[], "tags"=>["50", "hypomethylated", "mescs"], "article_id"=>354769, "categories"=>["Genetics"], "users"=>["Joanna Zhuang", "Allison Jones", "Shih-Han Lee", "Esther Ng", "Heidi Fiegl", "Michal Zikan", "David Cibula", "Alexandra Sargent", "Helga B. Salvesen", "Ian J. Jacobs", "Henry C. Kitchener", "Andrew E. Teschendorff", "Martin Widschwendter"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002517.g006", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Heatmap_of_the_top_50_most_frequently_hypomethylated_MESCs_in_cancers_/354769", "title"=>"Heatmap of the top 50 most frequently hypomethylated MESCs in cancers.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-02-09 01:19:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/683817"], "description"=>"<p>Bar charts representing percentages of significantly hypermethylated (blue) and hypomethylated (orange) PCGT and MESC CpGs in (A) each stage of cervical carcinogenesis: Cervix ‘Before Dysplasia’, ‘Dysplasia’, and ‘Invasive Cancer’, all relative to normal cervical cells or tissue; and in (B) ‘Breast CA’, ‘Endo CA’, ‘Colon CA’, and ‘Lung CA’, all relative to their respective normal controls. The significance of the binomial test assessing skew of hypermethylated versus hypomethylated is indicated by ‘*’, ‘**’, and ‘***’ for P-value<0.05, 0.01, and 0.001 respectively. (C) and (D) are the scatterplots of the age-adjusted linear regression t-statistics against their corresponding −log10(P-values) testing the association with the normal and lung cancer fibroblasts on the colon-PMD PCGTs and colon-PMD MESCs respectively.</p>", "links"=>[], "tags"=>["hypermethylated", "hypomethylated", "pcgts"], "article_id"=>354292, "categories"=>["Genetics"], "users"=>["Joanna Zhuang", "Allison Jones", "Shih-Han Lee", "Esther Ng", "Heidi Fiegl", "Michal Zikan", "David Cibula", "Alexandra Sargent", "Helga B. Salvesen", "Ian J. Jacobs", "Henry C. Kitchener", "Andrew E. Teschendorff", "Martin Widschwendter"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002517.g002", "stats"=>{"downloads"=>4, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Differential_dynamics_of_hypermethylated_and_hypomethylated_PCGTs_and_MESCs_/354292", "title"=>"Differential dynamics of hypermethylated and hypomethylated PCGTs and MESCs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-02-09 01:11:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/684619"], "description"=>"<p>Univariate and multivariate Cox regression results for the PCGT EpI and MESC EpI (DeMI) in endometrial, ovarian, cervical and breast cancer with number of samples (n), Hazard ratio (HR), 95% confidence interval (CI), and P-value (P). Overall survival was used for endometrial, ovarian and breast cancer, relapse free survival for cervical cancer.</p>", "links"=>[], "tags"=>["pcgts", "mescs", "epigenetic", "instability"], "article_id"=>355093, "categories"=>["Genetics"], "users"=>["Joanna Zhuang", "Allison Jones", "Shih-Han Lee", "Esther Ng", "Heidi Fiegl", "Michal Zikan", "David Cibula", "Alexandra Sargent", "Helga B. Salvesen", "Ian J. Jacobs", "Henry C. Kitchener", "Andrew E. Teschendorff", "Martin Widschwendter"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002517.t002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Survival_analysis_results_of_the_PCGTs_and_MESCs_Epigenetic_Instability_Index_EpI_in_women_s_cancers_/355093", "title"=>"Survival analysis results of the PCGTs and MESCs Epigenetic Instability Index (EpI) in women's cancers.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-02-09 01:24:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/683924"], "description"=>"<p>(A) Definition of epigenetic instability indices. Shown are the six possible DNA methylation changes between normal and cancer tissue. Thresholds used to define unmethylated (yellow), hemimethylated (skyblue) and fully methylated (blue) CpGs are described in <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002517#s4\" target=\"_blank\">Materials and Methods</a>. Stable MESC (or PCGT) CpGs are defined by MESC (or PCGT) CpGs, which have the same methylation state in all normal samples. The Epigenetic Instability Index (EpI) is then defined as the fraction of stable CpGs altered in cancer. We defined 4 separate indices to describe the transitions: 0→1/2, 1→2, 1→0, 2→1/0. The index describing alterations from a fully methylated to either a hemi or unmethylated state is called the Demethylation instability index (DeMI). (B) Dynamics of PCGT and MESC DNA methylation in cancer. Diagram illustrates the differential dynamics of PCGT and MESC CpG DNA methylation in cancer. Most PCGTs start out unmethylated (white lolly-pops) in normal cells but acquire DNA methylation (black lolly-pops) in normal cells 3 years before the emergence of dysplasia (BDy). PCGT hypermethylation increases further with Dysplasia (Dy) and cancer, but is not a strong determinant of invasion or poor outcome (metastasis). In contrast, most MESCs start out either fully or hemi methylated in normal cells, and gradually lose methylation during the progressive stages of cancer, with hypomethylation at MESCs a key determinant of metastases and poor outcome.</p>", "links"=>[], "tags"=>["epigenetic", "instability", "indices", "pcgt", "mesc", "methylation"], "article_id"=>354393, "categories"=>["Genetics"], "users"=>["Joanna Zhuang", "Allison Jones", "Shih-Han Lee", "Esther Ng", "Heidi Fiegl", "Michal Zikan", "David Cibula", "Alexandra Sargent", "Helga B. Salvesen", "Ian J. Jacobs", "Henry C. Kitchener", "Andrew E. Teschendorff", "Martin Widschwendter"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002517.g003", "stats"=>{"downloads"=>4, "page_views"=>26, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Definition_of_epigenetic_instability_indices_and_dynamics_of_PCGT_and_MESC_methylation_in_cancer_/354393", "title"=>"Definition of epigenetic instability indices and dynamics of PCGT and MESC methylation in cancer.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-02-09 01:13:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/684116"], "description"=>"<p>(A) Boxplots comparing the frequency of PCGT (0→1/2) DNA methylation changes, and the frequency of combined MESC (1→0) and MESC (2→0/1) DNA methylation changes (“combined DeMI index”), between normal endometrium (N), primary endometrial cancer (C), and between normal endometrium and metastatic endometrial cancers (MET). One-tailed Wilcoxon rank sum test P-values for the instability indices between cancer and metastases are indicated. (B) Receiver operating characteristic (ROC) curves measuring the dissimilarity in the combined DeMI index between primary and metastatic endometrial cancers with corresponding Area Under Curve (AUC) and 95% CI.</p>", "links"=>[], "tags"=>["changes", "metastatic", "endometrial"], "article_id"=>354587, "categories"=>["Genetics"], "users"=>["Joanna Zhuang", "Allison Jones", "Shih-Han Lee", "Esther Ng", "Heidi Fiegl", "Michal Zikan", "David Cibula", "Alexandra Sargent", "Helga B. Salvesen", "Ian J. Jacobs", "Henry C. Kitchener", "Andrew E. Teschendorff", "Martin Widschwendter"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002517.g005", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Methylation_changes_between_normal_primary_and_metastatic_endometrial_cancer_/354587", "title"=>"Methylation changes between normal, primary, and metastatic endometrial cancer.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-02-09 01:16:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/684435"], "description"=>"<p>Heatmap of the 250 most (upper half) and least (lower half) anti-correlated hypomethylated CpGs in cervical cancer samples ranked according to their TET3 mRNA expression levels from the lowest (green) to the highest (red) with the indication of MESCs (purple) and nonMESCs (white). The odds ratio (OR) and P-value (P) are obtained from Fisher's exact test estimating the enrichment of MESCs among hypomethylated CpGs that are significantly anti-correlated with TET3 mRNA expression level.</p>", "links"=>[], "tags"=>["tet", "mrna", "methylation"], "article_id"=>354911, "categories"=>["Genetics"], "users"=>["Joanna Zhuang", "Allison Jones", "Shih-Han Lee", "Esther Ng", "Heidi Fiegl", "Michal Zikan", "David Cibula", "Alexandra Sargent", "Helga B. Salvesen", "Ian J. Jacobs", "Henry C. Kitchener", "Andrew E. Teschendorff", "Martin Widschwendter"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1002517.g007", "stats"=>{"downloads"=>1, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Anti_correlation_between_the_TET_mRNA_expression_level_and_methylation_946_value_/354911", "title"=>"Anti-correlation between the TET mRNA expression level and methylation β-value.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-02-09 01:21:51"}

PMC Usage Stats | Further Information

  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"35", "year"=>"2012", "pdf"=>"22", "unique-ip"=>"33", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"7"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"71", "unique-ip"=>"68", "pdf"=>"44", "year"=>"2012", "figure"=>"25", "scanned-summary"=>"0", "supp-data"=>"13"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"46", "year"=>"2012", "pdf"=>"51", "unique-ip"=>"54", "figure"=>"10", "scanned-summary"=>"0", "supp-data"=>"4"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"56", "unique-ip"=>"60", "pdf"=>"42", "year"=>"2012", "figure"=>"21", "scanned-summary"=>"0", "supp-data"=>"15"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"43", "year"=>"2012", "pdf"=>"46", "unique-ip"=>"47", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"9"}
  • {"unique-ip"=>"28", "full-text"=>"26", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"32", "full-text"=>"32", "pdf"=>"18", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"30", "full-text"=>"32", "pdf"=>"28", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"42", "full-text"=>"42", "pdf"=>"21", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"22", "full-text"=>"13", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"19", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"23", "full-text"=>"25", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"26", "full-text"=>"29", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"30", "full-text"=>"36", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"23", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"33", "full-text"=>"41", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"32", "full-text"=>"37", "pdf"=>"10", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"21", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"21", "full-text"=>"25", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"19", "full-text"=>"16", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"20", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"21", "full-text"=>"21", "pdf"=>"16", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"16", "full-text"=>"21", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"18", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"5", "abstract"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"9", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"19", "full-text"=>"20", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"23", "full-text"=>"19", "pdf"=>"12", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"21", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"15", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"18", "full-text"=>"15", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"22", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"16", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"17", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"15", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"39", "cited-by"=>"1", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"19", "full-text"=>"17", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"7", "cited-by"=>"2", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"14", "full-text"=>"10", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"14", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"14", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"15", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"18", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"18", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"23", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"23", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"18", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"11", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"18", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"20", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"18", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"21", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"10"}

Relative Metric

{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Anatomy", "average_usage"=>[308, 530, 649, 751, 844, 933, 1021, 1107, 1194, 1277, 1357, 1426, 1501, 1572, 1634, 1702, 1768, 1838, 1910, 1976, 2046, 2114, 2181, 2241, 2298]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[319, 556, 679, 785, 881, 970, 1062, 1149, 1236, 1323, 1402, 1474, 1545, 1617, 1681, 1754, 1822, 1892, 1963, 2031, 2099, 2165, 2233, 2299, 2359]}, {"subject_area"=>"/Medicine and health sciences/Anatomy", "average_usage"=>[307, 529, 649, 751, 845, 936, 1025, 1113, 1197, 1284, 1360, 1432, 1501, 1570, 1632, 1697, 1765, 1834, 1904, 1972, 2041, 2107, 2170, 2231, 2292]}, {"subject_area"=>"/Medicine and health sciences/Health care", "average_usage"=>[335, 575, 699, 804, 900, 994, 1119, 1215, 1317, 1408, 1509, 1583, 1664, 1732, 1802, 1873, 1945, 2020, 2104, 2190, 2259, 2325, 2400, 2463, 2548]}]}
Loading … Spinner
There are currently no alerts