The NDR Kinase Scaffold HYM1/MO25 Is Essential for MAK2 MAP Kinase Signaling in Neurospora crassa
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"The NDR Kinase Scaffold HYM1/MO25 Is Essential for MAK2 MAP Kinase Signaling in Neurospora crassa", "type"=>"journal", "authors"=>[{"first_name"=>"Anne", "last_name"=>"Dettmann", "scopus_author_id"=>"35104476400"}, {"first_name"=>"Julia", "last_name"=>"Illgen", "scopus_author_id"=>"55370793800"}, {"first_name"=>"Sabine", "last_name"=>"März", "scopus_author_id"=>"35750441800"}, {"first_name"=>"Timo", "last_name"=>"Schürg", "scopus_author_id"=>"23567031000"}, {"first_name"=>"Andre", "last_name"=>"Fleissner", "scopus_author_id"=>"8375995200"}, {"first_name"=>"Stephan", "last_name"=>"Seiler", "scopus_author_id"=>"56363081500"}], "year"=>2012, "source"=>"PLoS Genetics", "identifiers"=>{"scopus"=>"2-s2.0-84866935357", "doi"=>"10.1371/journal.pgen.1002950", "sgr"=>"84866935357", "isbn"=>"1553-7404 (Electronic)\\n1553-7390 (Linking)", "pmid"=>"23028357", "issn"=>"15537390", "pui"=>"365755988"}, "id"=>"7d565ea3-b23e-3337-9de3-8986ca5f0fe0", "abstract"=>"Cell communication is essential for eukaryotic development, but our knowledge of molecules and mechanisms required for intercellular communication is fragmentary. In particular, the connection between signal sensing and regulation of cell polarity is poorly understood. In the filamentous ascomycete Neurospora crassa, germinating spores mutually attract each other and subsequently fuse. During these tropic interactions, the two communicating cells rapidly alternate between two different physiological states, probably associated with signal delivery and response. The MAK2 MAP kinase cascade mediates cell-cell signaling. Here, we show that the conserved scaffolding protein HYM1/MO25 controls the cell shape-regulating NDR kinase module as well as the signal-receiving MAP kinase cascade. HYM1 functions as an integral part of the COT1 NDR kinase complex to regulate the interaction with its upstream kinase POD6 and thereby COT1 activity. In addition, HYM1 interacts with NRC1, MEK2, and MAK2, the three kinases of the MAK2 MAP kinase cascade, and co-localizes with MAK2 at the apex of growing cells. During cell fusion, the three kinases of the MAP kinase module as well as HYM1 are recruited to the point of cell-cell contact. hym-1 mutants phenocopy all defects observed for MAK2 pathway mutants by abolishing MAK2 activity. An NRC1-MEK2 fusion protein reconstitutes MAK2 signaling in hym-1, while constitutive activation of NRC1 and MEK2 does not. These data identify HYM1 as a novel regulator of the NRC1-MEK2-MAK2 pathway, which may coordinate NDR and MAP kinase signaling during cell polarity and intercellular communication.", "link"=>"http://www.mendeley.com/research/ndr-kinase-scaffold-hym1mo25-essential-mak2-map-kinase-signaling-neurospora-crassa", "reader_count"=>29, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>3, "Student > Doctoral Student"=>3, "Researcher"=>5, "Student > Ph. D. Student"=>9, "Student > Postgraduate"=>2, "Student > Master"=>5, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>3, "Student > Doctoral Student"=>3, "Researcher"=>5, "Student > Ph. D. Student"=>9, "Student > Postgraduate"=>2, "Student > Master"=>5, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>7, "Agricultural and Biological Sciences"=>20, "Neuroscience"=>1}, "reader_count_by_subdiscipline"=>{"Neuroscience"=>{"Neuroscience"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>20}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>7}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"United States"=>1, "Brazil"=>1, "Portugal"=>1, "Germany"=>3}, "group_count"=>1}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84866935357"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84866935357?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84866935357&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84866935357&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84866935357", "dc:identifier"=>"SCOPUS_ID:84866935357", "eid"=>"2-s2.0-84866935357", "dc:title"=>"The NDR Kinase Scaffold HYM1/MO25 Is Essential for MAK2 MAP Kinase Signaling in Neurospora crassa", "dc:creator"=>"Dettmann A.", "prism:publicationName"=>"PLoS Genetics", "prism:issn"=>"15537390", "prism:eIssn"=>"15537404", "prism:volume"=>"8", "prism:issueIdentifier"=>"9", "prism:pageRange"=>nil, "prism:coverDate"=>"2012-09-01", "prism:coverDisplayDate"=>"September 2012", "prism:doi"=>"10.1371/journal.pgen.1002950", "citedby-count"=>"38", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Universität Göttingen", "affiliation-city"=>"Gottingen", "affiliation-country"=>"Germany"}], "pubmed-id"=>"23028357", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e1002950", "source-id"=>"4000151808", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosgenetics%2Farticle%3Fid%3D10.1371%252Fjournal.pgen.1002950", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Wikipedia | Further Information

Counter

  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"35", "xml_views"=>"7", "html_views"=>"431"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"51", "xml_views"=>"3", "html_views"=>"532"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"65"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"61"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"15", "xml_views"=>"2", "html_views"=>"209"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"42"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"86"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"40"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"93"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"62"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"75"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"54"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"81"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"51"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"53"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"72"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"91"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"72"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"83"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"15"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"3", "html_views"=>"7"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"2"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/303386", "https://ndownloader.figshare.com/files/303405", "https://ndownloader.figshare.com/files/303422", "https://ndownloader.figshare.com/files/303519", "https://ndownloader.figshare.com/files/303546", "https://ndownloader.figshare.com/files/303572", "https://ndownloader.figshare.com/files/303585", "https://ndownloader.figshare.com/files/303592", "https://ndownloader.figshare.com/files/303614", "https://ndownloader.figshare.com/files/303639", "https://ndownloader.figshare.com/files/303660", "https://ndownloader.figshare.com/files/303678"], "description"=>"<div><p>Cell communication is essential for eukaryotic development, but our knowledge of molecules and mechanisms required for intercellular communication is fragmentary. In particular, the connection between signal sensing and regulation of cell polarity is poorly understood. In the filamentous ascomycete <em>Neurospora crassa</em>, germinating spores mutually attract each other and subsequently fuse. During these tropic interactions, the two communicating cells rapidly alternate between two different physiological states, probably associated with signal delivery and response. The MAK2 MAP kinase cascade mediates cell–cell signaling. Here, we show that the conserved scaffolding protein HYM1/MO25 controls the cell shape-regulating NDR kinase module as well as the signal-receiving MAP kinase cascade. HYM1 functions as an integral part of the COT1 NDR kinase complex to regulate the interaction with its upstream kinase POD6 and thereby COT1 activity. In addition, HYM1 interacts with NRC1, MEK2, and MAK2, the three kinases of the MAK2 MAP kinase cascade, and co-localizes with MAK2 at the apex of growing cells. During cell fusion, the three kinases of the MAP kinase module as well as HYM1 are recruited to the point of cell–cell contact. <em>hym-1</em> mutants phenocopy all defects observed for MAK2 pathway mutants by abolishing MAK2 activity. An NRC1-MEK2 fusion protein reconstitutes MAK2 signaling in <em>hym-1</em>, while constitutive activation of NRC1 and MEK2 does not. These data identify HYM1 as a novel regulator of the NRC1-MEK2-MAK2 pathway, which may coordinate NDR and MAP kinase signaling during cell polarity and intercellular communication.</p> </div>", "links"=>[], "tags"=>["ndr", "kinase", "scaffold", "mak2", "signaling"], "article_id"=>119823, "categories"=>["Cell Biology", "Microbiology"], "users"=>["Anne Dettmann", "Julia Illgen", "Sabine März", "Timo Schürg", "Andre Fleissner", "Stephan Seiler"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002950.s001", "https://dx.doi.org/10.1371/journal.pgen.1002950.s002", "https://dx.doi.org/10.1371/journal.pgen.1002950.s003", "https://dx.doi.org/10.1371/journal.pgen.1002950.s004", "https://dx.doi.org/10.1371/journal.pgen.1002950.s005", "https://dx.doi.org/10.1371/journal.pgen.1002950.s006", "https://dx.doi.org/10.1371/journal.pgen.1002950.s007", "https://dx.doi.org/10.1371/journal.pgen.1002950.s008", "https://dx.doi.org/10.1371/journal.pgen.1002950.s009", "https://dx.doi.org/10.1371/journal.pgen.1002950.s010", "https://dx.doi.org/10.1371/journal.pgen.1002950.s011", "https://dx.doi.org/10.1371/journal.pgen.1002950.s012"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/The_NDR_Kinase_Scaffold_HYM1_MO25_Is_Essential_for_MAK2_MAP_Kinase_Signaling_in_Neurospora_crassa_/119823", "title"=>"The NDR Kinase Scaffold HYM1/MO25 Is Essential for MAK2 MAP Kinase Signaling in <em>Neurospora crassa</em>", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-09-20 02:43:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/572240"], "description"=>"<p>(A) Yeast two hybrid tests identify HYM1 as direct interaction partner of COT1 or POD6. Genes cloned into pGBKT7 and pGADT7 (mentioned as first or second label, respectively) were co-expressed as fusion proteins with the GAL4 DNA-binding domain and activation domains, respectively. Plasmids expressing the indicated proteins either as prey or as bait alone were used as negative controls and pGBKT7-53 (murine p53) and pGADT7-recT (SV40 large T antigen) fusion proteins as positive control. (B) Co-immunoprecipitation of GFP-tagged HYM1 recovered the small and large isoform of myc-COT1 and HA-POD6 from <i>N. crassa</i> cell extracts indicating the formation of a COT1-POD6-HYM1 complex <i>in vivo</i>. (C) Kinase activity assay of myc-COT1 purified from the indicated strains. Data represent means of five independent experiments (standard deviations are indicated as bars; the star denotes significantly different COT1 activity in the tested strains (p<0,01; unpaired Student's t-test). (D) Reciprocal anti-myc and anti-HA immunoprecipitation experiments from strains co-expressing myc-COT1 and HA-POD6 in a Δ<i>hym-1</i> background failed to recover HA-POD6 and myc-COT1, respectively. (E) COT1-GFP accumulated at the hyphal tip as bright spot at the distal side of the <i>Spitzenkörper</i> (co-stained with the marker dye FM4-64) and as apex-associated crescent. HYM1, in contrast, did not form this apex-associated crescent and its apical localization fully coincided with the <i>Spitzenkörper</i>. Note that both proteins co-localized at constricting septa (<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002950#pgen.1002950.s008\" target=\"_blank\">Videos S1</a>, <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002950#pgen.1002950.s009\" target=\"_blank\">S2</a>). COT1-GFP in a Δ<i>hym-1</i> background still accumulated as a bright spot at the distal side of the <i>Spitzenkörper</i> and as an apex-associated crescent at the hyphal tip.</p>", "links"=>[], "tags"=>["functions", "scaffold", "cot1-pod6"], "article_id"=>242727, "categories"=>["Cell Biology", "Microbiology"], "users"=>["Anne Dettmann", "Julia Illgen", "Sabine März", "Timo Schürg", "Andre Fleissner", "Stephan Seiler"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002950.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_HYM1_functions_as_scaffold_protein_for_the_COT1_POD6_complex_/242727", "title"=>"HYM1 functions as scaffold protein for the COT1-POD6 complex.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-09-20 00:45:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/572335"], "description"=>"<p>(A) Phenotypic characterization of Δ<i>hym-1</i>, Δ<i>mak-2</i> and wild type regarding macroscopic morphology and conidiation pattern (left panel: growth in slants for 5 days on minimal medium), hyphal morphology (upper right panel; bar), and protoperithecia formation (lower right panel). (B) Production of conidiospores was quantified by counting conidia generated in slants grown at room temperature for 5 days (n = 5; standard deviations are indicated as bars). (C) HYM1 and MAK2 are required for vegetative cell fusion. Hyphal (left panel) and germling fusion events (right panel; fusion events are indicated by arrows) were assessed by light microscopy in wild type, Δ<i>hym-1</i> and Δ<i>mak-2</i> cultures. Cell fusion was not observed in Δ<i>hym-1</i> and Δ<i>mak-2</i>. (D) Quantification of cell fusion competence and formation of forced heterokarya that grew on double-selective media supplemented with 150 µg/ml hygromycin and 20 µg/ml nourseothricin. 10<sup>4</sup> hygromycin-resistant conidiospores of the indicated strains (“wild type” carried an ectopically integrated (EC) hygromycin-resistance cassette, while the hygromycin-resistance cassette was used to replace the two gene deletions in Δ<i>hym-1</i> and Δ<i>mak-2</i>) were plated alone (second column) or in decreasing concentrations together with a second “wild type” strain that carried an ectopically integrated nourseothricin-resistance cassette (column 3–6). Column 1 indicated lack of growth of the “wild type” nat<sup>R</sup> control strain. 1% sorbose was added to Vogels minimal medium to restrict radial growth of the forming colonies generated by forced heterokaryons after incubation for 3 days at room temperature. (E) HYM1 is required for MAK2 activity. Total soluble protein was extracted from the indicated strains grown in the presence or absence of 8 mM H<sub>2</sub>O<sub>2</sub>. Western blot analysis with anti-phospho-p42/p44 antibody detected activated MAK1 and MAK2, respectively. Equal loading was confirmed by re-probing the membrane with anti-tubulin antibody. A typical Western blot is shown. MAK1 and MAK2 activities from 5 independent experiments were quantified for the diagrams.</p>", "links"=>[], "tags"=>["displays", "phenotypic", "characteristics", "mak2", "kinase", "pathway"], "article_id"=>242821, "categories"=>["Cell Biology", "Microbiology"], "users"=>["Anne Dettmann", "Julia Illgen", "Sabine März", "Timo Schürg", "Andre Fleissner", "Stephan Seiler"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002950.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_hym_1_displays_phenotypic_characteristics_of_MAK2_MAP_kinase_pathway_mutants_/242821", "title"=>"Δ<i>hym-1</i> displays phenotypic characteristics of MAK2 MAP kinase pathway mutants.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-09-20 00:47:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/572416"], "description"=>"<p>(A) Co-immunoprecipitation experiments of forced heterokaryons expressing myc-tagged HYM1 with flag-NRC1, flag-MEK2 or HA-MAK2. Interactions were observed between HYM1 and all three kinases of the MAK2 pathway. (B) Yeast two-hybrid analysis of HYM1 and the kinases NRC1, MEK2 and MAK2 revealed no physical interactions between the tested protein pairs. Interactions between NRC1 and MEK2 and between MEK2 and MAK2 were used as positive controls. Moreover, HYM1 and NRC1 interacted with the PAK kinase STE20, but not the homologous kinase CLA4.</p>", "links"=>[], "tags"=>["interacts", "components", "mak2", "kinase"], "article_id"=>242907, "categories"=>["Cell Biology", "Microbiology"], "users"=>["Anne Dettmann", "Julia Illgen", "Sabine März", "Timo Schürg", "Andre Fleissner", "Stephan Seiler"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002950.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_HYM1_interacts_with_components_of_the_MAK2_MAP_kinase_pathway_/242907", "title"=>"HYM1 interacts with components of the MAK2 MAP kinase pathway.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-09-20 00:48:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/572494"], "description"=>"<p>(A) Constitutive hyperactive versions of NRC1 (NRC1(P448S)) and MEK2 (MEK2(S212D;T216D) do not activate MAK2 in a Δ<i>hym-1</i> background. Total soluble protein was extracted from the indicated strains grown at 25°C and Western blot analyzed with anti-phospho-p42/p44 antibody to probe for MAK2 activity. Equal loading was confirmed by re-probing the membrane with anti-FLAG antibody. (B) Expression of a MEK2-NRC1 fusion protein in Δ<i>hym-1</i> resulted in wild type levels of active MAK2 (determined by Western blot experiments with anti-phospho-p42/p44 antibody). Note that the stability of the MEK2-NRC1 fusion protein seems reduced in Δ<i>hym-1</i>, resulting in the consistent appearance of potential degradation products with smaller size (B). An anti-tubulin blot served as loading control to indicate equal protein levels. (C) Expression of a MEK2-NRC1 fusion protein complemented all Δ<i>hym-1</i> defects (panel 1: aerial mycelium formation in slants; panel 2: sexual development/protoperithecia formation; panel 3: germling fusion; panel 4: growth rate).</p>", "links"=>[], "tags"=>["transduction", "mak2", "kinase"], "article_id"=>242989, "categories"=>["Cell Biology", "Microbiology"], "users"=>["Anne Dettmann", "Julia Illgen", "Sabine März", "Timo Schürg", "Andre Fleissner", "Stephan Seiler"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002950.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_HYM1_is_required_for_signal_transduction_through_the_entire_MAK2_kinase_cascade_/242989", "title"=>"HYM1 is required for signal transduction through the entire MAK2 kinase cascade.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-09-20 00:49:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/572600"], "description"=>"<p>(A) MAK2-GFP accumulated at the hyphal tip and the forming septum, while MEK2 and NRC1 only labeled the septum, but not the cell apex. Note that MAK2 has a cytosolic and nuclear distribution, while MEK2 and NRC1 are exclusive cytosolic proteins. Nuclei, hyphal plasma membrane/septa were co-labeled with histone H1-RFP and FM4-64, respectively, and detected in the red channel. (B) HYM1 and MAK2 co-localized in the <i>Spitzenkörper</i> at the hyphal tip and at constricting septa. Upper panel: comparison of hyphal tips of cells expressing HYM1-GFP and MAK2-GFP; lower panel: co-localization of co-expressed HYM1-GFP and MAK2-mCherry. (C) MAK2-GFP accumulated in the nucleus in a Δ<i>hym-1</i> background.</p>", "links"=>[], "tags"=>["mak2", "localization", "patterns", "vegetative"], "article_id"=>243092, "categories"=>["Cell Biology", "Microbiology"], "users"=>["Anne Dettmann", "Julia Illgen", "Sabine März", "Timo Schürg", "Andre Fleissner", "Stephan Seiler"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002950.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_NRC1_MEK2_and_MAK2_display_different_localization_patterns_in_vegetative_hyphae_/243092", "title"=>"NRC1, MEK2, and MAK2 display different localization patterns in vegetative hyphae.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-09-20 00:51:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/572800"], "description"=>"<p>Dynamic localization of MAK2-GFP, MEK2-GFP and NRC1-GFP at opposing tips of interacting germlings. HYM1-GFP is recruited to the contact zone of germling fusion pairs. COT1-GFP associated with both tips during tropic growth of communicating germlings.</p>", "links"=>[], "tags"=>["kinase", "cascade", "oscillate", "germling"], "article_id"=>243290, "categories"=>["Cell Biology", "Microbiology"], "users"=>["Anne Dettmann", "Julia Illgen", "Sabine März", "Timo Schürg", "Andre Fleissner", "Stephan Seiler"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002950.g006"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Components_of_the_MAP_kinase_cascade_oscillate_during_germling_fusion_/243290", "title"=>"Components of the MAP kinase cascade oscillate during germling fusion.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-09-20 00:54:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/572923"], "description"=>"<p>(A) MAK2-GFP and MEK2-mCherry recruitment to the germling tips oscillated synchronously during tropic growth. (B) MAK2-GFP and MEK2-mCherry co-localized in complexes at the tips of interacting germlings. Images were taken at one time point. DIC Inlay shows the entire germlings. (C) Model for the potential interactions of the COT1 and MAK2 signal pathways through the common scaffold HYM1; see text for details.</p>", "links"=>[], "tags"=>["mek2-mcherry", "co-localize", "tips", "interacting"], "article_id"=>243412, "categories"=>["Cell Biology", "Microbiology"], "users"=>["Anne Dettmann", "Julia Illgen", "Sabine März", "Timo Schürg", "Andre Fleissner", "Stephan Seiler"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002950.g007"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_MAK2_GFP_and_MEK2_mCherry_co_localize_at_the_tips_of_interacting_germlings_/243412", "title"=>"MAK2-GFP and MEK2-mCherry co-localize at the tips of interacting germlings.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-09-20 00:56:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/573061"], "description"=>"*<p>in cm/day.</p>**<p>Basal MAK2 activity was determined as described in Material and Methods; MAK2 activity was calculated relative to wild type, whose activity was set to 100% (n≥3).</p>", "links"=>[], "tags"=>["characteristics", "constitutive", "hyperactive", "nrc1", "mek2", "variants", "indicated"], "article_id"=>243545, "categories"=>["Cell Biology", "Microbiology"], "users"=>["Anne Dettmann", "Julia Illgen", "Sabine März", "Timo Schürg", "Andre Fleissner", "Stephan Seiler"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1002950.t001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Phenotypic_characteristics_of_constitutive_hyperactive_NRC1_and_MEK2_variants_in_the_indicated_strains_/243545", "title"=>"Phenotypic characteristics of constitutive hyperactive NRC1 and MEK2 variants in the indicated strains.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-09-20 00:59:05"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"26", "full-text"=>"20", "pdf"=>"18", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"22", "supp-data"=>"14", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"29", "full-text"=>"27", "pdf"=>"11", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"2", "cited-by"=>"2", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"17", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"24", "full-text"=>"25", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"30", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"16", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"20", "full-text"=>"17", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"0", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"4", "pdf"=>"0", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"14", "full-text"=>"9", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"17", "full-text"=>"18", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"12", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"16", "full-text"=>"23", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"19", "full-text"=>"21", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"6", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"5", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"17", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"19", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}

Relative Metric

{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[322, 550, 671, 773, 864, 955, 1048, 1135, 1223, 1308, 1387, 1465, 1534, 1602, 1673, 1744, 1813, 1885, 1955, 2026, 2093, 2160, 2228, 2290, 2349]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[319, 556, 679, 785, 881, 970, 1062, 1149, 1236, 1323, 1402, 1474, 1545, 1617, 1681, 1754, 1822, 1892, 1963, 2031, 2099, 2165, 2233, 2299, 2359]}]}
Loading … Spinner
There are currently no alerts