Genome-Wide Analysis of ZmDREB Genes and Their Association with Natural Variation in Drought Tolerance at Seedling Stage of Zea mays L
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{"title"=>"Genome-Wide Analysis of ZmDREB Genes and Their Association with Natural Variation in Drought Tolerance at Seedling Stage of Zea mays L", "type"=>"journal", "authors"=>[{"first_name"=>"Shengxue", "last_name"=>"Liu", "scopus_author_id"=>"55866272800"}, {"first_name"=>"Xianglan", "last_name"=>"Wang", "scopus_author_id"=>"57190665098"}, {"first_name"=>"Hongwei", "last_name"=>"Wang", "scopus_author_id"=>"55866355500"}, {"first_name"=>"Haibo", "last_name"=>"Xin", "scopus_author_id"=>"57197542248"}, {"first_name"=>"Xiaohong", "last_name"=>"Yang", "scopus_author_id"=>"57190193558"}, {"first_name"=>"Jianbing", "last_name"=>"Yan", "scopus_author_id"=>"14628001400"}, {"first_name"=>"Jiansheng", "last_name"=>"Li", "scopus_author_id"=>"56191397100"}, {"first_name"=>"Lam Son Phan", "last_name"=>"Tran", "scopus_author_id"=>"7102277565"}, {"first_name"=>"Kazuo", "last_name"=>"Shinozaki", "scopus_author_id"=>"22939092300"}, {"first_name"=>"Kazuko", "last_name"=>"Yamaguchi-Shinozaki", "scopus_author_id"=>"7006475058"}, {"first_name"=>"Feng", "last_name"=>"Qin", "scopus_author_id"=>"56566069100"}], "year"=>2013, "source"=>"PLoS Genetics", "identifiers"=>{"scopus"=>"2-s2.0-84884678620", "sgr"=>"84884678620", "issn"=>"15537390", "isbn"=>"1553-7404", "pmid"=>"24086146", "doi"=>"10.1371/journal.pgen.1003790", "pui"=>"369903947"}, "id"=>"4415733e-74fc-3f70-92e6-acd58eec888f", "abstract"=>"The worldwide production of maize (Zea mays L.) is frequently impacted by water scarcity and as a result, increased drought tolerance is a priority target in maize breeding programs. While DREB transcription factors have been demonstrated to play a central role in desiccation tolerance, whether or not natural sequence variations in these genes are associated with the phenotypic variability of this trait is largely unknown. In the present study, eighteen ZmDREB genes present in the maize B73 genome were cloned and systematically analyzed to determine their phylogenetic relationship, synteny with rice, maize and sorghum genomes; pattern of drought-responsive gene expression, and protein transactivation activity. Importantly, the association between the nucleic acid variation of each ZmDREB gene with drought tolerance was evaluated using a diverse population of maize consisting of 368 varieties from tropical and temperate regions. A significant association between the genetic variation of ZmDREB2.7 and drought tolerance at seedling stage was identified. Further analysis found that the DNA polymorphisms in the promoter region of ZmDREB2.7, but not the protein coding region itself, was associated with different levels of drought tolerance among maize varieties, likely due to distinct patterns of gene expression in response to drought stress. In vitro, protein-DNA binding assay demonstrated that ZmDREB2.7 protein could specifically interact with the target DNA sequences. The transgenic Arabidopsis overexpressing ZmDREB2.7 displayed enhanced tolerance to drought stress. Moreover, a favorable allele of ZmDREB2.7, identified in the drought-tolerant maize varieties, was effective in imparting plant tolerance to drought stress. Based upon these findings, we conclude that natural variation in the promoter of ZmDREB2.7 contributes to maize drought tolerance, and that the gene and its favorable allele may be an important genetic resource for the genetic improvement of drought tolerance in maize.", "link"=>"http://www.mendeley.com/research/genomewide-analysis-zmdreb-genes-association-natural-variation-drought-tolerance-seedling-stage-zea", "reader_count"=>139, "reader_count_by_academic_status"=>{"Unspecified"=>4, "Professor > Associate Professor"=>2, "Student > Doctoral Student"=>12, "Researcher"=>26, "Student > Ph. D. Student"=>51, "Student > Postgraduate"=>12, "Other"=>4, "Student > Master"=>18, "Student > Bachelor"=>6, "Lecturer"=>2, "Professor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>4, "Professor > Associate Professor"=>2, "Student > Doctoral Student"=>12, "Researcher"=>26, "Student > Ph. D. Student"=>51, "Student > Postgraduate"=>12, "Other"=>4, "Student > Master"=>18, "Student > Bachelor"=>6, "Lecturer"=>2, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>9, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>14, "Agricultural and Biological Sciences"=>110, "Medicine and Dentistry"=>1, "Psychology"=>1, "Chemistry"=>2, "Computer Science"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>2}, "Psychology"=>{"Psychology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>110}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>14}, "Unspecified"=>{"Unspecified"=>9}, "Environmental Science"=>{"Environmental Science"=>1}}, "reader_count_by_country"=>{"Netherlands"=>1, "United States"=>2, "Brazil"=>3, "Mexico"=>1, "Paraguay"=>1}, "group_count"=>6}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1216572"], "description"=>"<p>The phylogenetic tree was constructed based on the sequence alignments of sixty-six, full-length <i>DREB</i> genes from four species. The gene codes and names are illustrated in red for maize; black for rice; blue for sorghum; and green for <i>Arabidopsis</i>. The gene names used for <i>AtDREBs</i>, <i>OsDREB1s</i> and <i>OsDREB2s</i> were according to Sakuma et al., 2002 <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003790#pgen.1003790-Sakuma1\" target=\"_blank\">[7]</a>, Mao et al., 2012 <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003790#pgen.1003790-Mao1\" target=\"_blank\">[65]</a>, and Matsukura et al., 2010 <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003790#pgen.1003790-Matsukura1\" target=\"_blank\">[19]</a>. Genes contained within a blue-box were considered to be direct orthologous genes across species. Bootstrap values from 1,000 replicates were indicated at each node and the scale represents branch lengths.</p>", "links"=>[], "tags"=>["canonical", "genes", "sorghum"], "article_id"=>808783, "categories"=>["Biological Sciences", "Ecology"], "users"=>["Shengxue Liu", "Xianglan Wang", "Hongwei Wang", "Haibo Xin", "Xiaohong Yang", "Jianbing Yan", "Jiansheng Li", "Lam-Son Phan Tran", "Kazuo Shinozaki", "Kazuko Yamaguchi-Shinozaki", "Feng Qin"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1003790.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Phylogenetic_tree_of_canonical_DREB1_and_DREB2_genes_in_maize_rice_sorghum_and_Arabidopsis_/808783", "title"=>"Phylogenetic tree of canonical <i>DREB1</i> and <i>DREB2</i> genes in maize, rice, sorghum and <i>Arabidopsis</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-26 01:40:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/1216573"], "description"=>"<p>The maize, sorghum, and rice genomes are abbreviated as <i>Zm, Sb, Os</i>, respectively. Homologous chromosome segments between the different genomes are linked by black dotted lines. Each <i>DREB</i> orthologous gene pair is connected by a red line. Yellow boxes indicate homologous regions between the maize and sorghum genomes while gray boxes identify homologous regions in the maize and rice genomes. The blue shaded regions indicate two segmental duplications in the maize genome, corresponding to one rice and/or sorghum segment.</p>", "links"=>[], "tags"=>["chromosomal", "segments", "containing", "genes", "sorghum", "maize"], "article_id"=>808784, "categories"=>["Biological Sciences", "Ecology"], "users"=>["Shengxue Liu", "Xianglan Wang", "Hongwei Wang", "Haibo Xin", "Xiaohong Yang", "Jianbing Yan", "Jiansheng Li", "Lam-Son Phan Tran", "Kazuo Shinozaki", "Kazuko Yamaguchi-Shinozaki", "Feng Qin"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1003790.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Synteny_of_chromosomal_segments_containing_ZmDREB_genes_among_rice_sorghum_and_maize_genomes_/808784", "title"=>"Synteny of chromosomal segments containing <i>ZmDREB</i> genes among rice, sorghum and maize genomes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-26 01:40:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/1216574"], "description"=>"<p>(A) A heat map illustrating levels of gene expression level of the 18 <i>ZmDREB</i> genes in fifteen different tissues from various developmental stages. Normalized gene expression values are shown in different colors that represent the levels of expression indicated by the scale bar. The gray color represents unavailable data. (B) Relative levels of gene expression of 18 <i>ZmDREBs</i> in maize B73 leaf and root tissue grown under normal and drought conditions. The <i>ZmUbi-2</i> gene, which is constitutively expressed under a wide array of conditions, was used as an internal control. For leaf tissue, the collection time points, 5, 10, and 24 hours, reflected relative leaf water content (RLWC) of 70%, 60% and 58%, respectively. For the sampled root tissues, dehydration stress was applied to hydroponically cultured seedlings for 5, 10, and 24 hours, and RLWC was determined to be approximately 70%, 60% and 58% at the corresponding time points, respectively. Seedlings for the leaf studies were grown and drought stressed in soil (see <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003790#s4\" target=\"_blank\">Materials and Methods</a>). Data represent the mean ± SD of three biological replicates. (T-test, *p<0.05, **p<0.01).</p>", "links"=>[], "tags"=>["profiles", "eighteen"], "article_id"=>808785, "categories"=>["Biological Sciences", "Ecology"], "users"=>["Shengxue Liu", "Xianglan Wang", "Hongwei Wang", "Haibo Xin", "Xiaohong Yang", "Jianbing Yan", "Jiansheng Li", "Lam-Son Phan Tran", "Kazuo Shinozaki", "Kazuko Yamaguchi-Shinozaki", "Feng Qin"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1003790.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_profiles_of_eighteen_ZmDREB_genes_/808785", "title"=>"Expression profiles of eighteen <i>ZmDREB</i> genes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-26 01:40:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/1216575"], "description"=>"<p>(A) Cultures of the AH109 yeast, transformed with a plasmid containing different <i>ZmDREB</i> genes, were diluted and inoculated on to a synthetic dropout (SD) media without tryptophan (SD/-T), without tryptophan and histidine (SD/-T-H), or without tryptophan, histidine, and adenine (SD/-T-H-A). The culture plates were amended with different concentrations of 3-aminotriazole (3-AT). Genes were classified into one of three groups (I, II, or III) representing high, medium, or low transactivation activity, respectively. Photos were taken 2 days after inoculation for the plates without 3-AT, and 5 days after inoculation for the plates with various concentrations of 3-AT. (B) Motif analysis of ZmDREB proteins. Conserved protein motifs were identified using the SALAD database (<a href=\"http://salad.dna.affrc.go.jp/salad/\" target=\"_blank\">http://salad.dna.affrc.go.jp/salad/</a>). Different motifs were numbered from 1 to 26 and genes labeled with the same number(s) indicate that the same motif(s) was present in the different ZmDREB proteins. The conserved AP2/ERF domain is highlighted in red. Motifs labeled in purple were specific to ZmDREB1 group proteins, except that Motif 3 was also identified in the ZmDREB2.1/2A protein.</p>", "links"=>[], "tags"=>["assay", "motif", "18", "zmdreb"], "article_id"=>808786, "categories"=>["Biological Sciences", "Ecology"], "users"=>["Shengxue Liu", "Xianglan Wang", "Hongwei Wang", "Haibo Xin", "Xiaohong Yang", "Jianbing Yan", "Jiansheng Li", "Lam-Son Phan Tran", "Kazuo Shinozaki", "Kazuko Yamaguchi-Shinozaki", "Feng Qin"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1003790.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transactivation_activity_assay_and_motif_analysis_of_18_ZmDREB_proteins_/808786", "title"=>"Transactivation activity assay and motif analysis of 18 ZmDREB proteins.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-26 01:40:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/1216576"], "description"=>"<p>(A) Association analysis of genetic variation <i>ZmDREB2.7</i> with maize drought tolerance and the pattern of pairwise LD of DNA polymorphisms in the <i>ZmDREB2.7</i> gene. A schematic diagram of the 2.1 kb genomic fragment, including 600-bp 5′-, 406-bp 3′-UTR, and the protein coding region are presented as the x-axis. The location of the initiation codon (ATG) is marked as “+1”. The P value is shown on a −log<sub>10</sub> scale. The five significant polymorphisms in the 5′-UTR and four nonsynonymous variations in the coding region are connected to their locations on the gene diagram by solid lines. “<b>*</b>” indicates a strong LD (r<sup>2</sup>≥0.8) with these five polymorphisms. (B) Correlation analysis of survival rate with the relative expression level of <i>ZmDREB2.7</i>. Drought stress was applied to the maize seedlings after the RLWC was decreased from 98% (unstressed) to 70% (moderate drought) or 58% (severe drought).</p>", "links"=>[], "tags"=>["maize", "drought"], "article_id"=>808787, "categories"=>["Biological Sciences", "Ecology"], "users"=>["Shengxue Liu", "Xianglan Wang", "Hongwei Wang", "Haibo Xin", "Xiaohong Yang", "Jianbing Yan", "Jiansheng Li", "Lam-Son Phan Tran", "Kazuo Shinozaki", "Kazuko Yamaguchi-Shinozaki", "Feng Qin"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1003790.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Association_analysis_of_genetic_variation_ZmDREB2_7_with_maize_drought_tolerance_/808787", "title"=>"Association analysis of genetic variation <i>ZmDREB2.7</i> with maize drought tolerance.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-26 01:40:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/1216595"], "description"=>"<p>(A) (a) Dosage-dependent binding of GST-ZmDREB2.7 to DRE1 and DRE2 elements. GST-ZmDREB2.1/2A was used as a positive control. (b) Competitive DNA binding assay of ZmDREB2.7 with DRE1 and DRE2 sequences. 10-, 100-, 500-fold excess amounts of the cold probe were used to compete for the binding of ZmDREB2.7 protein to the labeled probe. (c) The binding assay of GST-ZmDREB2.7 with a GCC-box. The sequences of the three kinds of DNA sequences are listed. Red letters indicate the core sequences. DNA-protein complexes are indicated by arrows; “*” indicates nonspecific bands; “FP” indicates unbound free probes. (B) (a) Drought tolerance of transgenic <i>35S:ZmDREB2.7 Arabidopsis</i> plants. Photographs were taken both before and after the drought treatment followed by 6 days rewatering. Vector-transformed plants and <i>ZmDREB2.7</i>-<i>OE9</i>, <i>ZmDREB2.7</i>-<i>OE17</i> and <i>ZmDREB2.7</i>-<i>OE19</i> transgenic plants were compared. (b) RT-PCR analysis of transcript levels in the three lines of the <i>35S:ZmDREB2.7</i> transgenic plants. (c) Statistical analysis of survival rates after the drought-stress treatment. The average survival rates and standard errors were calculated from three independent experiments. Bars with asterisks indicate lines that had significantly higher survival rates than the vector-transformed plants (t-test, *p<0.05, **p<0.01).</p>", "links"=>[], "tags"=>["binding", "drought", "transgenic"], "article_id"=>808791, "categories"=>["Biological Sciences", "Ecology"], "users"=>["Shengxue Liu", "Xianglan Wang", "Hongwei Wang", "Haibo Xin", "Xiaohong Yang", "Jianbing Yan", "Jiansheng Li", "Lam-Son Phan Tran", "Kazuo Shinozaki", "Kazuko Yamaguchi-Shinozaki", "Feng Qin"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1003790.g006"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_DNA_binding_analysis_of_ZmDREB2_7_and_drought_stress_tolerance_of_35S_ZmDREB2_7_transgenic_Arabidopsis_plants_/808791", "title"=>"DNA binding analysis of ZmDREB2.7 and drought stress tolerance of <i>35S:ZmDREB2.7</i> transgenic <i>Arabidopsis</i> plants.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-26 01:40:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/1216601"], "description"=>"<p>(A) Haplotypes of <i>ZmDREB2.7</i> in CIMBL70, 91, 92, CML118, Shen5003 and B73 (as reference genome) maize genotypes. The site of the start codon (ATG) was designated as “+1”. SNP-503, SNP-260, SNP-150, InDel-185 and InDel-154 are the five DNA polymorphisms significantly associated with maize drought tolerance and are located in the 5′-UTR of <i>ZmDREB2.7</i>. The 20-bp InDel upstream of the ATG is in complete LD with the five polymorphisms in the four drought tolerant varieties. These polymorphisms are shaded in red. The location of PCR primers used for genotyping the InDel polymorphism of <i>ZmDREB2.7</i> in drought tolerant (CIMBL70, 91, 92, and CML118) and drought sensitive (Shen5003) inbred lines are indicated by arrows. The four significant nonsynonymous polymorphisms in the coding region of InDel141, SNP142, SNP436 and SNP661 are shaded in blue. (B) Phenotypic response of CIMBL70, 91, 92, CML118 and Shen5003 to drought stress. The upper panel is a photo of plants growing under favorable water conditions while the lower panel plants re-watered for 6 days after the drought stress treatment was terminated. (C) The survival rate of CIMBL70, 91, 92, CML118 and Shen5003 plants exposed to moderate and severe drought stress. Data represent the mean of triplicates (t-test, *p<0.05, **p<0.01). (D) Relative level of <i>ZmDREB2.7</i> expression in CIMBL70, 91, 92, CML118 and Shen5003 grown under normal and drought stress conditions. The drought-stress treatment reflected a decrease in RLWC from 98% (unstressed) to 70% (moderate drought), and 58% (severe drought). Data represent the mean of three biological replicates (t-test, **p<0.01). (E) The effect of the <i>ZmDREB2.7</i> favorable allele on drought tolerance in four F2 segregating populations of maize. In each population, three distinct genotypes for <i>ZmDREB2.7</i> were identified by DNA amplification: homozygous for the favorable allele, homozygous for the sensitive allele, and heterozygous for both alleles. The survival rate of the different genotypes was assessed and compared in the four populations. N indicates the number of F2 individuals tested in each population (t-test, *p<0.05, **p<0.01).</p>", "links"=>[], "tags"=>["allele", "improves", "maize", "drought"], "article_id"=>808797, "categories"=>["Biological Sciences", "Ecology"], "users"=>["Shengxue Liu", "Xianglan Wang", "Hongwei Wang", "Haibo Xin", "Xiaohong Yang", "Jianbing Yan", "Jiansheng Li", "Lam-Son Phan Tran", "Kazuo Shinozaki", "Kazuko Yamaguchi-Shinozaki", "Feng Qin"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1003790.g007"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_favorable_allele_of_ZmDREB2_7_improves_maize_drought_tolerance_/808797", "title"=>"The favorable allele of <i>ZmDREB2.7</i> improves maize drought tolerance.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-26 01:40:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/1216602"], "description"=>"*<p>MAF (Minor Allele Frequency)≥0.05; “-”data unavailable.</p>", "links"=>[], "tags"=>["genes", "drought", "seedling", "maize"], "article_id"=>808798, "categories"=>["Biological Sciences", "Ecology"], "users"=>["Shengxue Liu", "Xianglan Wang", "Hongwei Wang", "Haibo Xin", "Xiaohong Yang", "Jianbing Yan", "Jiansheng Li", "Lam-Son Phan Tran", "Kazuo Shinozaki", "Kazuko Yamaguchi-Shinozaki", "Feng Qin"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1003790.t001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Association_analysis_of_natural_variation_in_ZmDREB_genes_with_drought_tolerance_at_seedling_stage_in_the_maize_diversity_panel_/808798", "title"=>"Association analysis of natural variation in <i>ZmDREB</i> genes with drought tolerance at seedling stage in the maize diversity panel.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-09-26 01:40:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/1216607", "https://ndownloader.figshare.com/files/1216608", "https://ndownloader.figshare.com/files/1216609", "https://ndownloader.figshare.com/files/1216610", "https://ndownloader.figshare.com/files/1216611", "https://ndownloader.figshare.com/files/1216612", "https://ndownloader.figshare.com/files/1216613", "https://ndownloader.figshare.com/files/1216614", "https://ndownloader.figshare.com/files/1216615", "https://ndownloader.figshare.com/files/1216616", "https://ndownloader.figshare.com/files/1216617", "https://ndownloader.figshare.com/files/1216618", "https://ndownloader.figshare.com/files/1216619", "https://ndownloader.figshare.com/files/1216621"], "description"=>"<div><p>The worldwide production of maize (<i>Zea mays</i> L.) is frequently impacted by water scarcity and as a result, increased drought tolerance is a priority target in maize breeding programs. While DREB transcription factors have been demonstrated to play a central role in desiccation tolerance, whether or not natural sequence variations in these genes are associated with the phenotypic variability of this trait is largely unknown. In the present study, eighteen <i>ZmDREB</i> genes present in the maize B73 genome were cloned and systematically analyzed to determine their phylogenetic relationship, synteny with rice, maize and sorghum genomes; pattern of drought-responsive gene expression, and protein transactivation activity. Importantly, the association between the nucleic acid variation of each <i>ZmDREB</i> gene with drought tolerance was evaluated using a diverse population of maize consisting of 368 varieties from tropical and temperate regions. A significant association between the genetic variation of <i>ZmDREB2.7</i> and drought tolerance at seedling stage was identified. Further analysis found that the DNA polymorphisms in the promoter region of <i>ZmDREB2.7</i>, but not the protein coding region itself, was associated with different levels of drought tolerance among maize varieties, likely due to distinct patterns of gene expression in response to drought stress. <i>In vitro</i>, protein-DNA binding assay demonstrated that ZmDREB2.7 protein could specifically interact with the target DNA sequences. The transgenic <i>Arabidopsis</i> overexpressing <i>ZmDREB2.7</i> displayed enhanced tolerance to drought stress. Moreover, a favorable allele of <i>ZmDREB2.7</i>, identified in the drought-tolerant maize varieties, was effective in imparting plant tolerance to drought stress. Based upon these findings, we conclude that natural variation in the promoter of <i>ZmDREB2.7</i> contributes to maize drought tolerance, and that the gene and its favorable allele may be an important genetic resource for the genetic improvement of drought tolerance in maize.</p></div>", "links"=>[], "tags"=>["genome-wide", "genes", "drought", "seedling"], "article_id"=>808803, "categories"=>["Biological Sciences", "Ecology"], "users"=>["Shengxue Liu", "Xianglan Wang", "Hongwei Wang", "Haibo Xin", "Xiaohong Yang", "Jianbing Yan", "Jiansheng Li", "Lam-Son Phan Tran", "Kazuo Shinozaki", "Kazuko Yamaguchi-Shinozaki", "Feng Qin"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1003790.s001", "https://dx.doi.org/10.1371/journal.pgen.1003790.s002", "https://dx.doi.org/10.1371/journal.pgen.1003790.s003", "https://dx.doi.org/10.1371/journal.pgen.1003790.s004", "https://dx.doi.org/10.1371/journal.pgen.1003790.s005", "https://dx.doi.org/10.1371/journal.pgen.1003790.s006", "https://dx.doi.org/10.1371/journal.pgen.1003790.s007", "https://dx.doi.org/10.1371/journal.pgen.1003790.s008", "https://dx.doi.org/10.1371/journal.pgen.1003790.s009", "https://dx.doi.org/10.1371/journal.pgen.1003790.s010", "https://dx.doi.org/10.1371/journal.pgen.1003790.s011", "https://dx.doi.org/10.1371/journal.pgen.1003790.s012", "https://dx.doi.org/10.1371/journal.pgen.1003790.s013", "https://dx.doi.org/10.1371/journal.pgen.1003790.s014"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Genome_Wide_Analysis_of_ZmDREB_Genes_and_Their_Association_with_Natural_Variation_in_Drought_Tolerance_at_Seedling_Stage_of_Zea_mays_L/808803", "title"=>"Genome-Wide Analysis of <i>ZmDREB</i> Genes and Their Association with Natural Variation in Drought Tolerance at Seedling Stage of <i>Zea mays</i> L", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-09-26 01:40:46"}

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Relative Metric

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