Asymmetric Transcript Discovery by RNA-seq in C. elegans Blastomeres Identifies neg-1, a Gene Important for Anterior Morphogenesis
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Asymmetric Transcript Discovery by RNA-seq in C. elegans Blastomeres Identifies neg-1, a Gene Important for Anterior Morphogenesis", "type"=>"journal", "authors"=>[{"first_name"=>"Erin", "last_name"=>"Osborne Nishimura", "scopus_author_id"=>"55752428800"}, {"first_name"=>"Jay C.", "last_name"=>"Zhang", "scopus_author_id"=>"56667346800"}, {"first_name"=>"Adam D.", "last_name"=>"Werts", "scopus_author_id"=>"26633380000"}, {"first_name"=>"Bob", "last_name"=>"Goldstein", "scopus_author_id"=>"7402431287"}, {"first_name"=>"Jason D.", "last_name"=>"Lieb", "scopus_author_id"=>"7005815585"}], "year"=>2015, "source"=>"PLoS Genetics", "identifiers"=>{"scopus"=>"2-s2.0-84930366950", "issn"=>"15537404", "pui"=>"604707360", "isbn"=>"1553-7390", "sgr"=>"84930366950", "pmid"=>"25875092", "doi"=>"10.1371/journal.pgen.1005117"}, "id"=>"dc236721-47be-3328-9254-af0da626737a", "abstract"=>"After fertilization but prior to the onset of zygotic transcription, the C. elegans zygote cleaves asymmetrically to create the anterior AB and posterior P1 blastomeres, each of which goes on to generate distinct cell lineages. To understand how patterns of RNA inheritance and abundance arise after this first asymmetric cell division, we pooled hand-dissected AB and P1 blastomeres and performed RNA-seq. Our approach identified over 200 asymmetrically abundant mRNA transcripts. We confirmed symmetric or asymmetric abundance patterns for a subset of these transcripts using smFISH. smFISH also revealed heterogeneous subcellular patterning of the P1-enriched transcripts chs-1 and bpl-1. We screened transcripts enriched in a given blastomere for embryonic defects using RNAi. The gene neg-1 (F32D1.6) encoded an AB-enriched (anterior) transcript and was required for proper morphology of anterior tissues. In addition, analysis of the asymmetric transcripts yielded clues regarding the post-transcriptional mechanisms that control cellular mRNA abundance during asymmetric cell divisions, which are common in developing organisms.", "link"=>"http://www.mendeley.com/research/asymmetric-transcript-discovery-rnaseq-c-elegans-blastomeres-identifies-neg1-gene-important-anterior", "reader_count"=>38, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>3, "Researcher"=>16, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>1, "Student > Master"=>4, "Student > Bachelor"=>3, "Professor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>3, "Researcher"=>16, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>1, "Student > Master"=>4, "Student > Bachelor"=>3, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>6, "Agricultural and Biological Sciences"=>28, "Medicine and Dentistry"=>2, "Physics and Astronomy"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>28}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"Argentina"=>1, "Japan"=>1, "Mexico"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84930366950"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84930366950?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84930366950&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84930366950&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84930366950", "dc:identifier"=>"SCOPUS_ID:84930366950", "eid"=>"2-s2.0-84930366950", "dc:title"=>"Asymmetric Transcript Discovery by RNA-seq in C. elegans Blastomeres Identifies neg-1, a Gene Important for Anterior Morphogenesis", "dc:creator"=>"Osborne Nishimura E.", "prism:publicationName"=>"PLoS Genetics", "prism:issn"=>"15537390", "prism:eIssn"=>"15537404", "prism:volume"=>"11", "prism:issueIdentifier"=>"4", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-01-01", "prism:coverDisplayDate"=>"1 April 2015", "prism:doi"=>"10.1371/journal.pgen.1005117", "citedby-count"=>"12", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"The University of North Carolina at Chapel Hill", "affiliation-city"=>"Chapel Hill", "affiliation-country"=>"United States"}], "pubmed-id"=>"25875092", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e1005117", "source-id"=>"4000151808", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosgenetics%2Farticle%3Fid%3D10.1371%252Fjournal.pgen.1005117", "share_count"=>0, "like_count"=>198, "comment_count"=>33, "click_count"=>0, "total_count"=>231}

Counter

  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"141", "xml_views"=>"5", "html_views"=>"1546"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"57", "xml_views"=>"2", "html_views"=>"516"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"183"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"162"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"96"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"22", "xml_views"=>"1", "html_views"=>"137"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"111"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"90"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"85"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"109"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"28", "xml_views"=>"0", "html_views"=>"188"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"101"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"107"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"102"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"108"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"82"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"89"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"32", "xml_views"=>"0", "html_views"=>"141"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"164"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"135"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"146"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"18", "xml_views"=>"2", "html_views"=>"141"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"192"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"167"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"101"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"119"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"126"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"128"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"124"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"156"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"93"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"106"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"125"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"37", "xml_views"=>"0", "html_views"=>"94"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"37"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"3", "html_views"=>"24"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"10", "year"=>"2020", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"6"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2017033"], "description"=>"<p><b>(A-F)</b> Three AB-enriched, three P<sub>1</sub>-enriched, and three symmetric transcripts were imaged using sets of smFISH probes. <b>(A-B)</b> Images of wild type (<i>N2)</i> embryos grown at 20°C, fixed, and hybridized with <i>mex-3</i> or <i>neg-1</i> probes are shown. Ratios of particle density are reported (see <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005117#sec018\" target=\"_blank\">Materials and Methods</a>). Positive values indicate greater P<sub>1</sub> cell particle densities; negative values indicate greater AB cell densities. <i>neg-1</i> ranked #4 and <i>mex-3</i> ranked #42 of 80 AB-enriched transcripts when ordered by <i>P</i> value. <i>neg-1</i> (n = 11); <i>mex-3</i> (n = 10). <b>(C-F)</b><i>tes-1</i>, <i>chs-1</i>, <i>pgl-3</i>, and <i>bpl-1</i> were multiplexed with control probe sets labeled with a different fluorophore (hybridizing to either <i>gpd-2</i>, <i>set-3</i>, or <i>B0495</i>.<i>7)</i> to image both an asymmetric and symmetric transcript within the same embryo. <i>tes-</i>1 was ranked #77 of 80 AB-enriched transcripts. <i>chs-1</i> (rank #1), <i>pgl-3</i> (rank #32), and <i>bpl1</i> (rank #170) were among 201 P<sub>1</sub>-enriched transcripts. *<i>P</i> < 0.05; **<i>P</i> < 0.005, calculated using the Wilcoxon signed-rank test for paired samples. <i>tes-1</i> (n = 9); <i>chs-1</i> (n = 8); <i>pgl-3</i> (n = 7); <i>bpl-1</i> (n = 9). <b>(G)</b> P<sub>1</sub>-biased density of <i>pgl-3</i> particles was more dramatic in late 2-cell stage embryos undergoing mitosis. <b>(H)</b><i>chs-1</i> and <i>bpl-1</i> transcripts in fixed embryos over several stages of early embryonic development. All embryos are shown counterstained with DAPI. Maximal projections of optical sections are shown. Scale bars 5 μm.</p>", "links"=>[], "tags"=>["subcellular patterning", "gene neg", "elegans zygote cleaves", "Anterior Morphogenesis", "RNA inheritance", "cell lineages", "abundance patterns", "ab", "mRNA transcripts", "mRNA abundance", "cell divisions", "Asymmetric Transcript Discovery", "cell division", "F 32D", "P 1 blastomeres", "zygotic transcription"], "article_id"=>1377133, "categories"=>["Uncategorised"], "users"=>["Erin Osborne Nishimura", "Jay C. Zhang", "Adam D. Werts", "Bob Goldstein", "Jason D. Lieb"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005117.g003", "stats"=>{"downloads"=>0, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Quantitative_in_situ_hybridization_using_smFISH_probes_/1377133", "title"=>"Quantitative <i>in situ</i> hybridization using smFISH probes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-13 04:22:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/2017032"], "description"=>"<p><b>(A)</b> The 80 AB-enriched, the 201 P<sub>1</sub>-enriched, and a subset of the middle 80 symmetric transcripts (<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005117#pgen.1005117.s010\" target=\"_blank\">S3 Dataset</a>) were queried in a publically available collection of <i>in situ</i> hybridization images [<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005117#pgen.1005117.ref034\" target=\"_blank\">34</a>]. Pie charts show the proportion of transcripts with no entry (blue), successful staining (green), those that were in the database but showed no signal in any of the samples (No staining, red), and those that were otherwise interpretable due to overexposure, focus, or other problems (ambiguous, purple). Successfully stained entries were scored in a blind survey, and their results are plotted for each entry queried. Transcripts with scores higher than 2 are marked in blue and lower than 2 are marked in red. <b>(B)</b> Representative <i>in situ</i> hybridization images of AB-enriched transcripts [<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005117#pgen.1005117.ref034\" target=\"_blank\">34</a>]. The anterior AB cell is always oriented to the left of the posterior P<sub>1</sub> cell. Red arrowheads indicate the cell with higher observed signal. All images in B, C, and D were taken from the Nematode Expression Data Base (<a href=\"http://nematode.lab.nig.ac.jp/db2/index.php\" target=\"_blank\">http://nematode.lab.nig.ac.jp/db2/index.php</a>). <b>(C)</b> As in <b>B</b> for symmetric transcripts <b>(D)</b> As in <b>B</b> for P<sub>1</sub>-enriched transcripts. <b>(E)</b> qRT-PCR was performed on pools of 5 AB and matched P<sub>1</sub> cells for a subset of genes classified as AB-enriched (purple), P<sub>1</sub>-enriched (red), and symmetric (pink) by RNA-seq. Fold-change (P<sub>1</sub>/AB) as measured by qRT-PCR (Y-axis) and by RNA-seq (X-axis) is plotted. Each data point represents at least 2 independent biological samples, each of which was measured in at least 2 technical replicates; standard error of the mean is shown for biological replicates.</p>", "links"=>[], "tags"=>["subcellular patterning", "gene neg", "elegans zygote cleaves", "Anterior Morphogenesis", "RNA inheritance", "cell lineages", "abundance patterns", "ab", "mRNA transcripts", "mRNA abundance", "cell divisions", "Asymmetric Transcript Discovery", "cell division", "F 32D", "P 1 blastomeres", "zygotic transcription"], "article_id"=>1377132, "categories"=>["Uncategorised"], "users"=>["Erin Osborne Nishimura", "Jay C. Zhang", "Adam D. Werts", "Bob Goldstein", "Jason D. Lieb"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005117.g002", "stats"=>{"downloads"=>0, "page_views"=>22, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Complementary_methods_in_situ_hybridization_and_qRT_PCR_support_RNA_seq_measurements_/1377132", "title"=>"Complementary methods, <i>in situ</i> hybridization and qRT-PCR, support RNA-seq measurements.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-13 04:22:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/2017037", "https://ndownloader.figshare.com/files/2017038", "https://ndownloader.figshare.com/files/2017039", "https://ndownloader.figshare.com/files/2017040", "https://ndownloader.figshare.com/files/2017041", "https://ndownloader.figshare.com/files/2017042", "https://ndownloader.figshare.com/files/2017043", "https://ndownloader.figshare.com/files/2017044", "https://ndownloader.figshare.com/files/2017045", "https://ndownloader.figshare.com/files/2017046", "https://ndownloader.figshare.com/files/2017047", "https://ndownloader.figshare.com/files/2017048", "https://ndownloader.figshare.com/files/2017049", "https://ndownloader.figshare.com/files/2017050", "https://ndownloader.figshare.com/files/2017051", "https://ndownloader.figshare.com/files/2017052", "https://ndownloader.figshare.com/files/2017053", "https://ndownloader.figshare.com/files/2017054", "https://ndownloader.figshare.com/files/2017055", "https://ndownloader.figshare.com/files/2017056"], "description"=>"<div><p>After fertilization but prior to the onset of zygotic transcription, the <i>C</i>. <i>elegans</i> zygote cleaves asymmetrically to create the anterior AB and posterior P<sub>1</sub> blastomeres, each of which goes on to generate distinct cell lineages. To understand how patterns of RNA inheritance and abundance arise after this first asymmetric cell division, we pooled hand-dissected AB and P<sub>1</sub> blastomeres and performed RNA-seq. Our approach identified over 200 asymmetrically abundant mRNA transcripts. We confirmed symmetric or asymmetric abundance patterns for a subset of these transcripts using smFISH. smFISH also revealed heterogeneous subcellular patterning of the P<sub>1</sub>-enriched transcripts <i>chs-1</i> and <i>bpl-1</i>. We screened transcripts enriched in a given blastomere for embryonic defects using RNAi. The gene <i>neg-1 (F32D1</i>.<i>6)</i> encoded an AB-enriched (anterior) transcript and was required for proper morphology of anterior tissues. In addition, analysis of the asymmetric transcripts yielded clues regarding the post-transcriptional mechanisms that control cellular mRNA abundance during asymmetric cell divisions, which are common in developing organisms.</p></div>", "links"=>[], "tags"=>["subcellular patterning", "gene neg", "elegans zygote cleaves", "Anterior Morphogenesis", "RNA inheritance", "cell lineages", "abundance patterns", "ab", "mRNA transcripts", "mRNA abundance", "cell divisions", "Asymmetric Transcript Discovery", "cell division", "F 32D", "P 1 blastomeres", "zygotic transcription"], "article_id"=>1377137, "categories"=>["Uncategorised"], "users"=>["Erin Osborne Nishimura", "Jay C. Zhang", "Adam D. Werts", "Bob Goldstein", "Jason D. Lieb"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1005117.s001", "https://dx.doi.org/10.1371/journal.pgen.1005117.s002", "https://dx.doi.org/10.1371/journal.pgen.1005117.s003", "https://dx.doi.org/10.1371/journal.pgen.1005117.s004", "https://dx.doi.org/10.1371/journal.pgen.1005117.s005", "https://dx.doi.org/10.1371/journal.pgen.1005117.s006", "https://dx.doi.org/10.1371/journal.pgen.1005117.s007", "https://dx.doi.org/10.1371/journal.pgen.1005117.s008", "https://dx.doi.org/10.1371/journal.pgen.1005117.s009", "https://dx.doi.org/10.1371/journal.pgen.1005117.s010", "https://dx.doi.org/10.1371/journal.pgen.1005117.s011", "https://dx.doi.org/10.1371/journal.pgen.1005117.s012", "https://dx.doi.org/10.1371/journal.pgen.1005117.s013", "https://dx.doi.org/10.1371/journal.pgen.1005117.s014", "https://dx.doi.org/10.1371/journal.pgen.1005117.s015", "https://dx.doi.org/10.1371/journal.pgen.1005117.s016", "https://dx.doi.org/10.1371/journal.pgen.1005117.s017", "https://dx.doi.org/10.1371/journal.pgen.1005117.s018", "https://dx.doi.org/10.1371/journal.pgen.1005117.s019", "https://dx.doi.org/10.1371/journal.pgen.1005117.s020"], "stats"=>{"downloads"=>12, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Asymmetric_Transcript_Discovery_by_RNA_seq_in_C_elegans_Blastomeres_Identifies_neg_1_a_Gene_Important_for_Anterior_Morphogenesis/1377137", "title"=>"Asymmetric Transcript Discovery by RNA-seq in <i>C</i>. <i>elegans</i> Blastomeres Identifies <i>neg-1</i>, a Gene Important for Anterior Morphogenesis", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-04-13 04:22:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/2017035"], "description"=>"<p><b>(A)</b> GO ontology terms over-represented in genes whose transcripts were AB-enriched at the 2-cell stage of development. Categories were identified using the GOrilla algorithm for identifying categorical significance from ranked lists [<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005117#pgen.1005117.ref050\" target=\"_blank\">50</a>] and were summarized using the REViGO algorithm [<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005117#pgen.1005117.ref051\" target=\"_blank\">51</a>]. <b>(B)</b> Same as A for P<sub>1</sub>-enriched.</p>", "links"=>[], "tags"=>["subcellular patterning", "gene neg", "elegans zygote cleaves", "Anterior Morphogenesis", "RNA inheritance", "cell lineages", "abundance patterns", "ab", "mRNA transcripts", "mRNA abundance", "cell divisions", "Asymmetric Transcript Discovery", "cell division", "F 32D", "P 1 blastomeres", "zygotic transcription"], "article_id"=>1377135, "categories"=>["Uncategorised"], "users"=>["Erin Osborne Nishimura", "Jay C. Zhang", "Adam D. Werts", "Bob Goldstein", "Jason D. Lieb"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005117.g005", "stats"=>{"downloads"=>1, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Functions_associated_with_AB_and_P_1_enriched_transcripts_/1377135", "title"=>"Functions associated with AB and P<sub>1</sub>-enriched transcripts.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-13 04:22:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/2017036"], "description"=>"<p><b>(A)</b> The prevalence of Maternal Degradation [MD] transcripts (n = 1812) [<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005117#pgen.1005117.ref060\" target=\"_blank\">60</a>] among AB-enriched (n = 80), P<sub>1</sub>-enriched (n = 201), and symmetric transcripts (n = 2815). MD transcripts make up 19.8% of genes expressed at the 2-cell stage (horizontal red line). <b>(B)</b> Overlap between the P<sub>1</sub>-enriched gene set and transcripts enriched in Z<sub>2</sub> and Z<sub>3</sub> cells [<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005117#pgen.1005117.ref061\" target=\"_blank\">61</a>]. All individual genes that overlap are listed. <b>(C)</b> Association of cell-type specific transcripts [<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005117#pgen.1005117.ref061\" target=\"_blank\">61</a>] with the AB-enriched and P<sub>1</sub>-enriched gene sets. (*<i>P</i> < 5x10<sup>-2</sup>; **<i>P</i> < 5x10<sup>-3</sup>; ***<i>P</i> < 5x10<sup>-9</sup>, by Fisher’s exact test). Squares on the left indicate the lineage of origin for each tissue or cell type (blue = AB-lineage, blue and red = AB and P<sub>1</sub> lineages, red = P<sub>1</sub>-lineage). For comparison, overlap between the MD transcripts (Fig 6A) is also plotted. <b>(D)</b> Lengths of spliced gene models for AB-enriched genes and P<sub>1</sub>-enriched genes plotted as a histogram. The <i>P</i> value for the likelihood that the two distributions were drawn from the same population was calculated using Wilcoxon rank-sum (Mann-Whitney <i>U</i>) test with continuity correction (<i>P</i> < 1.0x10<sup>-16</sup> for all genes and <i>P</i> = 1.22x10<sup>-15</sup> when the three outlying AB-enriched gene models were excluded). <b>(E)</b> Identification of <i>de novo</i> RNA sequence motifs specific to the 3′ UTRs of P<sub>1</sub>-enriched transcripts as compared to symmetric transcripts [<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005117#pgen.1005117.ref062\" target=\"_blank\">62</a>]. No significant motifs were identified between AB-enriched transcripts compared to symmetric transcripts. <b>(F)</b> POS-1 and MEX-3 proteins are known to be asymmetrically localized at the 2-cell stage of development. The consensus sequences required for POS-1 [<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005117#pgen.1005117.ref063\" target=\"_blank\">63</a>] and MEX-3 [<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005117#pgen.1005117.ref064\" target=\"_blank\">64</a>] recognition are shown as IUPAC codes and transformed into WEBLOGOs for ease of comparison with <i>de novo</i> logos (Fig 6E).</p>", "links"=>[], "tags"=>["subcellular patterning", "gene neg", "elegans zygote cleaves", "Anterior Morphogenesis", "RNA inheritance", "cell lineages", "abundance patterns", "ab", "mRNA transcripts", "mRNA abundance", "cell divisions", "Asymmetric Transcript Discovery", "cell division", "F 32D", "P 1 blastomeres", "zygotic transcription"], "article_id"=>1377136, "categories"=>["Uncategorised"], "users"=>["Erin Osborne Nishimura", "Jay C. Zhang", "Adam D. Werts", "Bob Goldstein", "Jason D. Lieb"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005117.g006", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Characteristics_of_asymmetrically_abundant_genes_/1377136", "title"=>"Characteristics of asymmetrically abundant genes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-13 04:22:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/2017034"], "description"=>"<p><b>(A)</b> The embryonic lethality resulting from RNAi depletion of asymmetrically abundant transcripts in wild type <i>(N2)</i> and RNAi sensitive <i>(rrf-3)</i> strains is shown. Embryonic lethality is depicted using the illustrated colorscale. Samples not tested are colored in gray. Worms that showed reduced brood size in response to RNAi feeding are marked with a red asterisk. <b>(B)</b><i>neg-1</i> dsRNA injection is lethal and cold sensitive. Worms were grown at 20°C, injected with dsRNA or left uninjected at L4, and then moved to 15°C, 20°C, or 25°C. Embryonic lethality for the resulting embryos was scored for 3–4 replicates of 6 worms each. <b>(C)</b> Time-lapse microscopy of embryonic development from <i>neg-1</i> dsRNA-injected mothers at 20°C. Wild type embryos hatched normally under these conditions, but <i>neg-1</i> depleted embryos exhibited a range of elongation phenotypes from showing no signs of elongation, to partial elongation, to complete elongation. <b>(D)</b> Tabulation of embryo developmental phenotypes shown in (C). <b>(E)</b><i>dlg-1</i>::<i>GFP</i> embryos depleted of <i>neg-1</i> were imaged and compared by time lapse microscopy at 20°C to wild type embryos. <i>dlg-1</i>::<i>GFP</i> is a fluorescent marker for apical adherens junctions of the hypodermal and gut epithelial cells. <i>neg-1</i> depletion disrupted ventral enclosure. Leader cells are noted by yellow arrowheads. <b>(F)</b> Tabulation of the <i>dlg-1</i>::<i>GFP</i> phenotypes shown in (E). Hypodermal cell defects were predictive of a failure to hatch. (G) Visualization of <i>neg-1</i> mRNA transcripts in fixed, wild type (<i>N2</i>) embryos using smFISH probes hybridized to <i>neg-1</i> and DAPI as a nuclear stain. Scale bar 5 μm. Below, live embryos expressing NEONGREEN::NEG-1 driven from a construct that included the full length NEG-1 3’UTR and 100 bp of downstream sequence.</p>", "links"=>[], "tags"=>["subcellular patterning", "gene neg", "elegans zygote cleaves", "Anterior Morphogenesis", "RNA inheritance", "cell lineages", "abundance patterns", "ab", "mRNA transcripts", "mRNA abundance", "cell divisions", "Asymmetric Transcript Discovery", "cell division", "F 32D", "P 1 blastomeres", "zygotic transcription"], "article_id"=>1377134, "categories"=>["Uncategorised"], "users"=>["Erin Osborne Nishimura", "Jay C. Zhang", "Adam D. Werts", "Bob Goldstein", "Jason D. Lieb"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005117.g004", "stats"=>{"downloads"=>1, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_neg_1_is_required_for_morphogenesis_/1377134", "title"=>"<i>neg-1</i> is required for morphogenesis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-13 04:22:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/2017031"], "description"=>"<p><b>(A)</b><i>C</i>. <i>elegans</i> wild type embryos were harvested from gravid adults. AB and P<sub>1</sub> blastomeres were separated by microdissection, and mRNA was extracted, amplified and sequenced. <b>(B)</b> The first cell division is asymmetric and creates the anterior AB cell and posterior P<sub>1</sub> cell. RNA in the AB and P<sub>1</sub> cells is exclusively maternal prior to the onset of zygotic transcription (dashed line, shading). Symmetric cell divisions are marked by diamonds. <b>(C)</b> For each gene, the normalized ratio of RNA-seq read counts between P<sub>1</sub> and AB is plotted against the mean normalized read count for each transcript (<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005117#sec018\" target=\"_blank\">Materials and Methods</a>). Asymmetrically abundant transcripts are shown in blue (AB-enriched) and red (P<sub>1</sub>-enriched). <b>(D)</b> RNA-amp-seq traces for an AB-enriched transcript (<i>neg-1</i>, boxed in blue) <b>(E)</b> Same as <b>D</b> for a symmetric transcript (<i>tba-1)</i><b>(F)</b> Same as <b>D</b> for a P<sub>1</sub>-enriched transcript (<i>pgl-3)</i>.</p>", "links"=>[], "tags"=>["subcellular patterning", "gene neg", "elegans zygote cleaves", "Anterior Morphogenesis", "RNA inheritance", "cell lineages", "abundance patterns", "ab", "mRNA transcripts", "mRNA abundance", "cell divisions", "Asymmetric Transcript Discovery", "cell division", "F 32D", "P 1 blastomeres", "zygotic transcription"], "article_id"=>1377131, "categories"=>["Uncategorised"], "users"=>["Erin Osborne Nishimura", "Jay C. Zhang", "Adam D. Werts", "Bob Goldstein", "Jason D. Lieb"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005117.g001", "stats"=>{"downloads"=>1, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Differential_transcript_abundance_in_AB_and_P_1_blastomeres_following_the_first_embryonic_division_/1377131", "title"=>"Differential transcript abundance in AB and P<sub>1</sub> blastomeres following the first embryonic division.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-13 04:22:38"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"17", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"40", "full-text"=>"33", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"26", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"41", "full-text"=>"40", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"33", "full-text"=>"32", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"21", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"31", "full-text"=>"23", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"27", "full-text"=>"28", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"20", "full-text"=>"19", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"29", "full-text"=>"32", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"19", "full-text"=>"23", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"24", "full-text"=>"25", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"22", "full-text"=>"14", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"21", "full-text"=>"20", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"17", "full-text"=>"15", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"16", "full-text"=>"17", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"8", "cited-by"=>"1", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"17", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"16", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"20", "full-text"=>"26", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"18", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"11", "cited-by"=>"1", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"24", "full-text"=>"15", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"12", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"13", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"16", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"18", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"11", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"9"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts