Nmf9 Encodes a Highly Conserved Protein Important to Neurological Function in Mice and Flies
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{"title"=>"Nmf9 Encodes a Highly Conserved Protein Important to Neurological Function in Mice and Flies", "type"=>"journal", "authors"=>[{"first_name"=>"Shuxiao", "last_name"=>"Zhang", "scopus_author_id"=>"56767244100"}, {"first_name"=>"Kevin D.", "last_name"=>"Ross", "scopus_author_id"=>"56666857700"}, {"first_name"=>"Glen A.", "last_name"=>"Seidner", "scopus_author_id"=>"6506969528"}, {"first_name"=>"Michael R.", "last_name"=>"Gorman", "scopus_author_id"=>"56767649900"}, {"first_name"=>"Tiffany H.", "last_name"=>"Poon", "scopus_author_id"=>"36772243100"}, {"first_name"=>"Xiaobo", "last_name"=>"Wang", "scopus_author_id"=>"56767440300"}, {"first_name"=>"Elizabeth M.", "last_name"=>"Keithley", "scopus_author_id"=>"7006138555"}, {"first_name"=>"Patricia N.", "last_name"=>"Lee", "scopus_author_id"=>"7406116286"}, {"first_name"=>"Mark Q.", "last_name"=>"Martindale", "scopus_author_id"=>"7005752690"}, {"first_name"=>"William J.", "last_name"=>"Joiner", "scopus_author_id"=>"6701543231"}, {"first_name"=>"Bruce A.", "last_name"=>"Hamilton", "scopus_author_id"=>"7202534481"}], "year"=>2015, "source"=>"PLoS Genetics", "identifiers"=>{"issn"=>"15537404", "scopus"=>"2-s2.0-84938814625", "sgr"=>"84938814625", "pui"=>"605567928", "isbn"=>"1553-7404 (Electronic)\\r1553-7390 (Linking)", "pmid"=>"26131556", "doi"=>"10.1371/journal.pgen.1005344"}, "id"=>"1c59e8b2-2fa5-31fa-803c-bad7e3651da0", "abstract"=>"Many protein-coding genes identified by genome sequencing remain without functional annotation or biological context. Here we define a novel protein-coding gene, Nmf9, based on a forward genetic screen for neurological function. ENU-induced and genome-edited null mutations in mice produce deficits in vestibular function, fear learning and circadian behavior, which correlated with Nmf9 expression in inner ear, amygdala, and suprachiasmatic nuclei. Homologous genes from unicellular organisms and invertebrate animals predict interactions with small GTPases, but the corresponding domains are absent in mammalian Nmf9. Intriguingly, homozygotes for null mutations in the Drosophila homolog, CG45058, show profound locomotor defects and premature death, while heterozygotes show striking effects on sleep and activity phenotypes. These results link a novel gene orthology group to discrete neurological functions, and show conserved requirement across wide phylogenetic distance and domain level structural changes.", "link"=>"http://www.mendeley.com/research/nmf9-encodes-highly-conserved-protein-important-neurological-function-mice-flies-2", "reader_count"=>5, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>1, "Student > Ph. D. Student"=>1, "Student > Master"=>1, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>1, "Student > Ph. D. Student"=>1, "Student > Master"=>1, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>3}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2157884", "https://ndownloader.figshare.com/files/2157885", "https://ndownloader.figshare.com/files/2157886", "https://ndownloader.figshare.com/files/2157887", "https://ndownloader.figshare.com/files/2157888", "https://ndownloader.figshare.com/files/2157889", "https://ndownloader.figshare.com/files/2157890", "https://ndownloader.figshare.com/files/2157891", "https://ndownloader.figshare.com/files/2157892", "https://ndownloader.figshare.com/files/2157893", "https://ndownloader.figshare.com/files/2157894", "https://ndownloader.figshare.com/files/2157895", "https://ndownloader.figshare.com/files/2157896", "https://ndownloader.figshare.com/files/2157897", "https://ndownloader.figshare.com/files/2157898"], "description"=>"<div><p>Many protein-coding genes identified by genome sequencing remain without functional annotation or biological context. Here we define a novel protein-coding gene, <i>Nmf9</i>, based on a forward genetic screen for neurological function. ENU-induced and genome-edited null mutations in mice produce deficits in vestibular function, fear learning and circadian behavior, which correlated with <i>Nmf9</i> expression in inner ear, amygdala, and suprachiasmatic nuclei. Homologous genes from unicellular organisms and invertebrate animals predict interactions with small GTPases, but the corresponding domains are absent in mammalian <i>Nmf9</i>. Intriguingly, homozygotes for null mutations in the <i>Drosophila</i> homolog, <i>CG45058</i>, show profound locomotor defects and premature death, while heterozygotes show striking effects on sleep and activity phenotypes. These results link a novel gene orthology group to discrete neurological functions, and show conserved requirement across wide phylogenetic distance and domain level structural changes.</p></div>", "links"=>[], "tags"=>["Nmf 9 expression", "Nmf 9 Encodes", "Genome sequencing", "activity phenotypes", "heterozygotes show", "Neurological Function", "vestibular function", "results link", "unicellular organisms", "locomotor defects", "invertebrate animals", "circadian behavior", "Homologous genes", "mutation", "cg", "suprachiasmatic nuclei", "Conserved Protein", "Drosophila homolog", "phylogenetic distance", "domain level", "novel gene orthology group", "Nmf 9. Intriguingly"], "article_id"=>1470246, "categories"=>["Uncategorised"], "users"=>["Shuxiao Zhang", "Kevin D. Ross", "Glen A. Seidner", "Michael R. Gorman", "Tiffany H. Poon", "Xiaobo Wang", "Elizabeth M. Keithley", "Patricia N. Lee", "Mark Q. Martindale", "William J. Joiner", "Bruce A. Hamilton"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1005344.s001", "https://dx.doi.org/10.1371/journal.pgen.1005344.s002", "https://dx.doi.org/10.1371/journal.pgen.1005344.s003", "https://dx.doi.org/10.1371/journal.pgen.1005344.s004", "https://dx.doi.org/10.1371/journal.pgen.1005344.s005", "https://dx.doi.org/10.1371/journal.pgen.1005344.s006", "https://dx.doi.org/10.1371/journal.pgen.1005344.s007", "https://dx.doi.org/10.1371/journal.pgen.1005344.s008", "https://dx.doi.org/10.1371/journal.pgen.1005344.s009", "https://dx.doi.org/10.1371/journal.pgen.1005344.s010", "https://dx.doi.org/10.1371/journal.pgen.1005344.s011", "https://dx.doi.org/10.1371/journal.pgen.1005344.s012", "https://dx.doi.org/10.1371/journal.pgen.1005344.s013", "https://dx.doi.org/10.1371/journal.pgen.1005344.s014", "https://dx.doi.org/10.1371/journal.pgen.1005344.s015"], "stats"=>{"downloads"=>40, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Nmf9_Encodes_a_Highly_Conserved_Protein_Important_to_Neurological_Function_in_Mice_and_Flies/1470246", "title"=>"<i>Nmf9</i> Encodes a Highly Conserved Protein Important to Neurological Function in Mice and Flies", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-07-01 02:51:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/2157858"], "description"=>"<p>(A) Landing test showed increased frequency of rotation or curling by <i>nmf9</i> homozygotes relative to control (+/+) littermates (Fisher's exact test, p = 1.9x10<sup>-8</sup> between genotypes, 0.52 between sexes). (B) Forced swim test further showed vestibular dysfunction in <i>nmf9</i> relative to littermates (Asymptotic logrank test, p = 9.5x10<sup>-8</sup> for geneotype stratified by sex) with a greater performance difference in females (p = 0.017 for sex stratified by genotype) due to lower performance among control males. Red, control; pink, mutant females. Navy, control; blue, mutant males. (C) Circling by mutant animals also showed significant sex bias (Fisher’s exact test, p = 0.021) and age-dependent progression (p = 0.013) in frequency. Sample sizes apply to panels C-F. (D) Head nodding also supported progression (p = 0.0014), but not significant sex bias (p = 0.12). Although (E) hyperactivity and (F) tremor were pronounced in mutant animals, effects of sex and age on did not reach statistical significance.</p>", "links"=>[], "tags"=>["Nmf 9 expression", "Nmf 9 Encodes", "Genome sequencing", "activity phenotypes", "heterozygotes show", "Neurological Function", "vestibular function", "results link", "unicellular organisms", "locomotor defects", "invertebrate animals", "circadian behavior", "Homologous genes", "mutation", "cg", "suprachiasmatic nuclei", "Conserved Protein", "Drosophila homolog", "phylogenetic distance", "domain level", "novel gene orthology group", "Nmf 9. Intriguingly"], "article_id"=>1470226, "categories"=>["Uncategorised"], "users"=>["Shuxiao Zhang", "Kevin D. Ross", "Glen A. Seidner", "Michael R. Gorman", "Tiffany H. Poon", "Xiaobo Wang", "Elizabeth M. Keithley", "Patricia N. Lee", "Mark Q. Martindale", "William J. Joiner", "Bruce A. Hamilton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005344.g001", "stats"=>{"downloads"=>0, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mutant_nmf9_animals_show_age_and_sex_dependent_vestibular_dysfunction_/1470226", "title"=>"Mutant <i>nmf9</i> animals show age and sex-dependent vestibular dysfunction.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-01 02:51:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/2157859"], "description"=>"<p>(A) Recombination mapping places the <i>nmf9</i> mutation within a 1.3 Mb interval on Chr. 11. (B) Aligned UCSC Genome Browser window shows known and predicted genes in the non-recombining <i>nmf9</i> interval. Extent of EST support indicates poor sampling of putative genes <i>4932411E22Rik</i> and <i>Ankfn1</i>. (C) Sanger sequencing across the exon-intron junction shows a G-to-A transition in the essential GU splice donor adjacent to a frame-shifting exon in <i>Ankfn1</i>. (D) Schematic shows alignment of gene annotations from the UCSC genome browser (purple) with empirically determined transcripts (black) based on RT-PCR assays (numbered lines). Amplification and sequence analysis of assay 2 showed both inclusion of <i>Ankfn1</i> and <i>E22</i> in a single transcript in littermate control samples (+) and selective skipping of the mutated exon (red) in mutant samples (<i>n</i>). Additional assays indicated based on conservation, predicted exons and 5’ RACE showed variable inclusion of exons 5’ to the <i>Ankfn1</i> annotation. (E) Northern blot shows a single major band for <i>Nmf9</i> poly(A)<sup>+</sup> RNA from brain ~9 kb, with reduced level in <i>nmf9</i> mutant (<i>n</i>) compared to control (+) littermate brain RNA.</p>", "links"=>[], "tags"=>["Nmf 9 expression", "Nmf 9 Encodes", "Genome sequencing", "activity phenotypes", "heterozygotes show", "Neurological Function", "vestibular function", "results link", "unicellular organisms", "locomotor defects", "invertebrate animals", "circadian behavior", "Homologous genes", "mutation", "cg", "suprachiasmatic nuclei", "Conserved Protein", "Drosophila homolog", "phylogenetic distance", "domain level", "novel gene orthology group", "Nmf 9. Intriguingly"], "article_id"=>1470227, "categories"=>["Uncategorised"], "users"=>["Shuxiao Zhang", "Kevin D. Ross", "Glen A. Seidner", "Michael R. Gorman", "Tiffany H. Poon", "Xiaobo Wang", "Elizabeth M. Keithley", "Patricia N. Lee", "Mark Q. Martindale", "William J. Joiner", "Bruce A. Hamilton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005344.g002", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Positional_cloning_identifies_Nmf9_/1470227", "title"=>"Positional cloning identifies <i>Nmf9</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-01 02:51:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/2157860"], "description"=>"<p>(A) <i>In situ</i> hybridization to adjacent sections in E14.5 and E16.5 embryos show that <i>Nmf9</i> and <i>Atoh1</i>, a marker of hair cell progenitor were expressed in the same population of cells (indicated with arrows). Scale bar = 500 μm. (B) Panels from the Allen Brain Atlas [<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005344#pgen.1005344.ref025\" target=\"_blank\">25</a>] show <i>Nmf9</i> RNA enriched in distinct structures of adult brain, including accessory olfactory bulb (OB), piriform cortex (PC), lateral septum (LS), amygdala (AMY), suprachiasmatic nucleus (SCN) and Ventromedial hypothalamus (VMH).</p>", "links"=>[], "tags"=>["Nmf 9 expression", "Nmf 9 Encodes", "Genome sequencing", "activity phenotypes", "heterozygotes show", "Neurological Function", "vestibular function", "results link", "unicellular organisms", "locomotor defects", "invertebrate animals", "circadian behavior", "Homologous genes", "mutation", "cg", "suprachiasmatic nuclei", "Conserved Protein", "Drosophila homolog", "phylogenetic distance", "domain level", "novel gene orthology group", "Nmf 9. Intriguingly"], "article_id"=>1470228, "categories"=>["Uncategorised"], "users"=>["Shuxiao Zhang", "Kevin D. Ross", "Glen A. Seidner", "Michael R. Gorman", "Tiffany H. Poon", "Xiaobo Wang", "Elizabeth M. Keithley", "Patricia N. Lee", "Mark Q. Martindale", "William J. Joiner", "Bruce A. Hamilton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005344.g003", "stats"=>{"downloads"=>2, "page_views"=>31, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Nmf9_expression_pattern_predicts_new_sites_of_function_/1470228", "title"=>"<i>Nmf9</i> expression pattern predicts new sites of function.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-01 02:51:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/2157861"], "description"=>"<p>(A) Mutant animals showed decreased freezing behavior in both tone cued and contextual fear conditioning. (2-factor ANOVA p = 0.0018 for genotypes in contextual conditioning, 0.002 in tone-cued conditioning. Sex effects were non-significant, p = 0.29 in contextual, p = 0.09 in tone-cued, N = 14 +/+ female, 8 mutant female, 11 +/+ male, 8 mutant male, although the interaction between genotype and sex was supported for contextual fear conditioning, p = 0.026.) (B) Double-plotted actograms of running-wheel counts of mutant and wildtype from the same litter housed in 12 hour light: 12 hour dark (LD) cycle and continuous dark (DD) illustrates less robust circadian rhythm in a more severely affected animal. Arrowheads indicate transfer to DD. (C) Mutants showed a small but significant decrease in period length (p = 0.014) and an increase in period onset error (p = 0.034) during DD (2-factor ANOVA; effects of sex p = 0.14 for period length, p = 0.012 for onset error, with larger deviation in females. N = 14 wildtype females, 12 mutant females, 9 wildtype males, 10 mutant males).</p>", "links"=>[], "tags"=>["Nmf 9 expression", "Nmf 9 Encodes", "Genome sequencing", "activity phenotypes", "heterozygotes show", "Neurological Function", "vestibular function", "results link", "unicellular organisms", "locomotor defects", "invertebrate animals", "circadian behavior", "Homologous genes", "mutation", "cg", "suprachiasmatic nuclei", "Conserved Protein", "Drosophila homolog", "phylogenetic distance", "domain level", "novel gene orthology group", "Nmf 9. Intriguingly"], "article_id"=>1470229, "categories"=>["Uncategorised"], "users"=>["Shuxiao Zhang", "Kevin D. Ross", "Glen A. Seidner", "Michael R. Gorman", "Tiffany H. Poon", "Xiaobo Wang", "Elizabeth M. Keithley", "Patricia N. Lee", "Mark Q. Martindale", "William J. Joiner", "Bruce A. Hamilton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005344.g004", "stats"=>{"downloads"=>2, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Nmf9_is_important_to_fear_learning_and_circadian_rhythm_/1470229", "title"=>"<i>Nmf9</i> is important to fear learning and circadian rhythm.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-01 02:51:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/2157862"], "description"=>"<p>Behaviors that depend on <i>Nmf9</i>-expressing circuits were assayed and assessed by 2-factor ANOVA or MANOVA for sex and genotype. Number tested for each group (N) is indicated in each bar or a legend to line graphs. (A) Elevated Plus Maze behaviors were not significantly different between control (+/+) and mutant (n/n) littermates (Percent time in open arm p = 0.27 for genotype, p = 0.22 for sex; Number of transitions p = 0.12 for genotype, p = 0.23 for sex). (B) In the Open Field Test, mutant animals showed increased time spent in center, but potentially confounded by increased number of line crosses due to hyperactivity (Time in center p = 0.0043 for genotype, p = 0.82 for sex; number of transitions, 3.4x10<sup>-7</sup> for genotype, p = 0.0004 for sex). (C) No significant difference between genotypes was detected for the Marble Burying Test (p = 0.15 and 0.68 for genotype and sex, respectively). (D) While mutant females took somewhat longer in olfactory-dependent Buried Food Finding Test, this was did not reach conventional significance (p = 0.11 for genotype, 0.09 for sex). (E) Similarly, Odor Habituation / Dishabituation Tests did not support a significant difference (MANOVA, p = 0.69 for genotype, p = 0.30 for sex). (F) Weight was significantly different by genotype at 2 months (ANOVA, p = 0.014) and remained significant at 6 months (p = 0.0088), including well-known sex differences, while food consumption (G) was not significantly different (p = 0.11 for genotype, 0.75 for sex).</p>", "links"=>[], "tags"=>["Nmf 9 expression", "Nmf 9 Encodes", "Genome sequencing", "activity phenotypes", "heterozygotes show", "Neurological Function", "vestibular function", "results link", "unicellular organisms", "locomotor defects", "invertebrate animals", "circadian behavior", "Homologous genes", "mutation", "cg", "suprachiasmatic nuclei", "Conserved Protein", "Drosophila homolog", "phylogenetic distance", "domain level", "novel gene orthology group", "Nmf 9. Intriguingly"], "article_id"=>1470230, "categories"=>["Uncategorised"], "users"=>["Shuxiao Zhang", "Kevin D. Ross", "Glen A. Seidner", "Michael R. Gorman", "Tiffany H. Poon", "Xiaobo Wang", "Elizabeth M. Keithley", "Patricia N. Lee", "Mark Q. Martindale", "William J. Joiner", "Bruce A. Hamilton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005344.g005", "stats"=>{"downloads"=>0, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Tests_of_LS_PC_olfactory_pathway_and_VMH_/1470230", "title"=>"Tests of LS, PC/olfactory pathway, and VMH.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-01 02:51:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/2157863"], "description"=>"<p>(A) Nmf9 homologs exist for all branches of Metazoa (except Urochordates) and several non-animal Holozoa. A single example in fungi (<i>Mortierella verticillata</i>) may represent horizontal gene transfer. (B) Sliding window analysis of 14 approximately full-length metazoan homologs highlights known motifs and predicts three novel domains where the most highly conserved individual residues tend to cluster. Threshold line (red) indicates 25% percentile of values. Aligned schematic shows motif annotations (rectangles) and constrained intervals (ovals) in relation to the sliding window plot. (C) The most highly conserved amino acid sequence (red box) was in domain 2 and encoded by the exon skipped in <i>nmf9</i> mRNA.</p>", "links"=>[], "tags"=>["Nmf 9 expression", "Nmf 9 Encodes", "Genome sequencing", "activity phenotypes", "heterozygotes show", "Neurological Function", "vestibular function", "results link", "unicellular organisms", "locomotor defects", "invertebrate animals", "circadian behavior", "Homologous genes", "mutation", "cg", "suprachiasmatic nuclei", "Conserved Protein", "Drosophila homolog", "phylogenetic distance", "domain level", "novel gene orthology group", "Nmf 9. Intriguingly"], "article_id"=>1470231, "categories"=>["Uncategorised"], "users"=>["Shuxiao Zhang", "Kevin D. Ross", "Glen A. Seidner", "Michael R. Gorman", "Tiffany H. Poon", "Xiaobo Wang", "Elizabeth M. Keithley", "Patricia N. Lee", "Mark Q. Martindale", "William J. Joiner", "Bruce A. Hamilton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005344.g006", "stats"=>{"downloads"=>1, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Nmf9_protein_is_highly_conserved_in_Metazoans_/1470231", "title"=>"Nmf9 protein is highly conserved in Metazoans.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-01 02:51:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/2157864"], "description"=>"<p>(A) CRISPR/Cas9 target sequence is underlined, PAM site boxed, and predicted cut site shown as an arrow. Sequences of four induced alleles selected for further analysis are shown; deleted bases are indicated by dashes and insertions/substitutions by red lower-case letters. The first glycine residue, targeted for replacement, was at position 592 relative to RefSeq protein NP_001074402. (B) Results of the landing test with induced mutations heterozygous either to a coisogenic nonmutant allele (+/n) or to the original <i>nmf9</i> Jackson allele (n<sup>J</sup>/n<sup>C</sup>) showed non-complementation (p = 5.3x10<sup>-6</sup>, Fisher’s exact test). Similarly, head nodding (p = 1.3x10<sup>-5</sup>), tremor (3.6x10<sup>-4</sup>), and hyperactivity (p = 0.0053) were more frequently observed in trans-heterozygotes for <i>nmf9</i> and CRISPR-induced alleles than in controls. (C) Time-to-event in the forced swim tests also showed non-complementation between edited and the original <i>nmf9</i> alleles (Asymptotic logrank test, p = 0.0011).</p>", "links"=>[], "tags"=>["Nmf 9 expression", "Nmf 9 Encodes", "Genome sequencing", "activity phenotypes", "heterozygotes show", "Neurological Function", "vestibular function", "results link", "unicellular organisms", "locomotor defects", "invertebrate animals", "circadian behavior", "Homologous genes", "mutation", "cg", "suprachiasmatic nuclei", "Conserved Protein", "Drosophila homolog", "phylogenetic distance", "domain level", "novel gene orthology group", "Nmf 9. Intriguingly"], "article_id"=>1470232, "categories"=>["Uncategorised"], "users"=>["Shuxiao Zhang", "Kevin D. Ross", "Glen A. Seidner", "Michael R. Gorman", "Tiffany H. Poon", "Xiaobo Wang", "Elizabeth M. Keithley", "Patricia N. Lee", "Mark Q. Martindale", "William J. Joiner", "Bruce A. Hamilton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005344.g007", "stats"=>{"downloads"=>2, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Genome_edited_alleles_show_importance_of_conserved_domain_2_and_confirm_identity_of_nmf9_/1470232", "title"=>"Genome edited alleles show importance of conserved domain 2 and confirm identity of <i>nmf9</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-01 02:51:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/2157865"], "description"=>"<p>(A) Target sites and recovered alleles are shown for separately targeted exons encoding the first ANK repeat, FN3 domain, and the GLYLGYLK peptide. (B) Posture of a heterozygous control fly. (C) Posture of a mutant fly homozygous for a frame-shifting mutation in conserved domain 2. Note wings held up at an angle with respect to the body. (D) The same mutant fly subsequently fell over and moved legs in an uncoordinated fashion. (E) Survival frequencies at eclosion and 7 days post-eclosion. (F) Total waking activity (beam breaks per minutes awake) for males (blue) and females (pink) of the indicated genotypes. Heterozygotes for framshifting mutations in <i>CG45058</i> ANK, FN3 or conserved domain 2 consistently showed reduced activity relative to +/+ control, similar in magnitude to <i>wake</i> (EY02219) homozygotes. *** p<2.2x10<sup>-16</sup>.</p>", "links"=>[], "tags"=>["Nmf 9 expression", "Nmf 9 Encodes", "Genome sequencing", "activity phenotypes", "heterozygotes show", "Neurological Function", "vestibular function", "results link", "unicellular organisms", "locomotor defects", "invertebrate animals", "circadian behavior", "Homologous genes", "mutation", "cg", "suprachiasmatic nuclei", "Conserved Protein", "Drosophila homolog", "phylogenetic distance", "domain level", "novel gene orthology group", "Nmf 9. Intriguingly"], "article_id"=>1470233, "categories"=>["Uncategorised"], "users"=>["Shuxiao Zhang", "Kevin D. Ross", "Glen A. Seidner", "Michael R. Gorman", "Tiffany H. Poon", "Xiaobo Wang", "Elizabeth M. Keithley", "Patricia N. Lee", "Mark Q. Martindale", "William J. Joiner", "Bruce A. Hamilton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005344.g008", "stats"=>{"downloads"=>0, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Genome_editing_of_Drosophila_CG45058_exons_results_in_severe_locomotor_deficits_and_early_death_/1470233", "title"=>"Genome editing of <i>Drosophila CG45058</i> exons results in severe locomotor deficits and early death.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-01 02:51:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/2157866"], "description"=>"<p>Male heterozygotes (blue graphs) recorded less total sleep per 24 hr. period and slightly longer latency to first sleep bout after lights on (daytime sleep latency) than +/+ controls, but no significant difference in latency after lights off (nighttime sleep latency). By contrast female heterozygotes (pink graphs) recorded slightly more overall sleep than +/+ controls and slightly longer daytime and nighttime sleep latencies. Heterozygous <i>wake</i> P-element alleles (EY02219, KG02188, KG08407 and MI02905 insertion lines) and a homozygous <i>wake</i> allele (EY02219) were recorded as a comparison. * p<0.025, *** p<5x10<sup>-4</sup>, ns p = 0.78, Wilcoxon rank sum test.</p>", "links"=>[], "tags"=>["Nmf 9 expression", "Nmf 9 Encodes", "Genome sequencing", "activity phenotypes", "heterozygotes show", "Neurological Function", "vestibular function", "results link", "unicellular organisms", "locomotor defects", "invertebrate animals", "circadian behavior", "Homologous genes", "mutation", "cg", "suprachiasmatic nuclei", "Conserved Protein", "Drosophila homolog", "phylogenetic distance", "domain level", "novel gene orthology group", "Nmf 9. Intriguingly"], "article_id"=>1470234, "categories"=>["Uncategorised"], "users"=>["Shuxiao Zhang", "Kevin D. Ross", "Glen A. Seidner", "Michael R. Gorman", "Tiffany H. Poon", "Xiaobo Wang", "Elizabeth M. Keithley", "Patricia N. Lee", "Mark Q. Martindale", "William J. Joiner", "Bruce A. Hamilton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005344.g009", "stats"=>{"downloads"=>1, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_CG45058_null_heterozygotes_have_abnormal_sleep_patterns_/1470234", "title"=>"<i>CG45058</i> null heterozygotes have abnormal sleep patterns.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-01 02:51:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/2157867"], "description"=>"<p>Phenotypes are noted as 2 for strong, 1 for mild, or 0 for not evident.</p><p>Mouse <i>nmf9</i> alleles generated by CRISPR/Cas9-mediated genome editing.</p>", "links"=>[], "tags"=>["Nmf 9 expression", "Nmf 9 Encodes", "Genome sequencing", "activity phenotypes", "heterozygotes show", "Neurological Function", "vestibular function", "results link", "unicellular organisms", "locomotor defects", "invertebrate animals", "circadian behavior", "Homologous genes", "mutation", "cg", "suprachiasmatic nuclei", "Conserved Protein", "Drosophila homolog", "phylogenetic distance", "domain level", "novel gene orthology group", "Nmf 9. Intriguingly"], "article_id"=>1470235, "categories"=>["Uncategorised"], "users"=>["Shuxiao Zhang", "Kevin D. Ross", "Glen A. Seidner", "Michael R. Gorman", "Tiffany H. Poon", "Xiaobo Wang", "Elizabeth M. Keithley", "Patricia N. Lee", "Mark Q. Martindale", "William J. Joiner", "Bruce A. Hamilton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005344.t001", "stats"=>{"downloads"=>4, "page_views"=>29, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mouse_nmf9_alleles_generated_by_CRISPR_Cas9_mediated_genome_editing_/1470235", "title"=>"Mouse <i>nmf9</i> alleles generated by CRISPR/Cas9-mediated genome editing.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-07-01 02:51:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/2157868"], "description"=>"<p>Frequency with which animals of the indicated genotype were called abnormal by a trained observer blind to genotype is indicated for each test and allele combination, as is the average number of the five tests for which a given genotype was considered abnormal. P-values are for the one-sided Fisher’s Exact Test of the null hypothesis that mutant/<i>nmf9</i> heterozygotes are not more often affected than mutant/+ heterozygotes, based on sums across all five induced alleles.</p><p>Allele-level data and p-values for non-complementation of <i>nmf9</i> by CRISPR-induced mutations.</p>", "links"=>[], "tags"=>["Nmf 9 expression", "Nmf 9 Encodes", "Genome sequencing", "activity phenotypes", "heterozygotes show", "Neurological Function", "vestibular function", "results link", "unicellular organisms", "locomotor defects", "invertebrate animals", "circadian behavior", "Homologous genes", "mutation", "cg", "suprachiasmatic nuclei", "Conserved Protein", "Drosophila homolog", "phylogenetic distance", "domain level", "novel gene orthology group", "Nmf 9. Intriguingly"], "article_id"=>1470236, "categories"=>["Uncategorised"], "users"=>["Shuxiao Zhang", "Kevin D. Ross", "Glen A. Seidner", "Michael R. Gorman", "Tiffany H. Poon", "Xiaobo Wang", "Elizabeth M. Keithley", "Patricia N. Lee", "Mark Q. Martindale", "William J. Joiner", "Bruce A. Hamilton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005344.t002", "stats"=>{"downloads"=>1, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Allele_level_data_and_p_values_for_non_complementation_of_nmf9_by_CRISPR_induced_mutations_/1470236", "title"=>"Allele-level data and p-values for non-complementation of <i>nmf9</i> by CRISPR-induced mutations.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-07-01 02:51:01"}

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  • {"unique-ip"=>"11", "full-text"=>"15", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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