Outer Hair Cell Lateral Wall Structure Constrains the Mobility of Plasma Membrane Proteins
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{"title"=>"Outer Hair Cell Lateral Wall Structure Constrains the Mobility of Plasma Membrane Proteins", "type"=>"journal", "authors"=>[{"first_name"=>"Tetsuji", "last_name"=>"Yamashita", "scopus_author_id"=>"55366558300"}, {"first_name"=>"Pierre", "last_name"=>"Hakizimana", "scopus_author_id"=>"24314533500"}, {"first_name"=>"Siva", "last_name"=>"Wu", "scopus_author_id"=>"14023827800"}, {"first_name"=>"Ahmed", "last_name"=>"Hassan", "scopus_author_id"=>"56370759300"}, {"first_name"=>"Stefan", "last_name"=>"Jacob", "scopus_author_id"=>"16549532600"}, {"first_name"=>"Jamshid", "last_name"=>"Temirov", "scopus_author_id"=>"6507433028"}, {"first_name"=>"Jie", "last_name"=>"Fang", "scopus_author_id"=>"35232978800"}, {"first_name"=>"Marcia", "last_name"=>"Mellado-Lagarde", "scopus_author_id"=>"23470181100"}, {"first_name"=>"Richard", "last_name"=>"Gursky", "scopus_author_id"=>"57194643815"}, {"first_name"=>"Linda", "last_name"=>"Horner", "scopus_author_id"=>"57078339500"}, {"first_name"=>"Barbara", "last_name"=>"Leibiger", "scopus_author_id"=>"6603955390"}, {"first_name"=>"Sara", "last_name"=>"Leijon", "scopus_author_id"=>"54079867500"}, {"first_name"=>"Victoria E.", "last_name"=>"Centonze", "scopus_author_id"=>"56730364900"}, {"first_name"=>"Per Olof", "last_name"=>"Berggren", "scopus_author_id"=>"7101706643"}, {"first_name"=>"Sharon", "last_name"=>"Frase", "scopus_author_id"=>"6602609111"}, {"first_name"=>"Manfred", "last_name"=>"Auer", "scopus_author_id"=>"7006393995"}, {"first_name"=>"William E.", "last_name"=>"Brownell", "scopus_author_id"=>"7006844559"}, {"first_name"=>"Anders", "last_name"=>"Fridberger", "scopus_author_id"=>"6701661816"}, {"first_name"=>"Jian", "last_name"=>"Zuo", "scopus_author_id"=>"7202871946"}], "year"=>2015, "source"=>"PLoS Genetics", "identifiers"=>{"issn"=>"15537404", "scopus"=>"2-s2.0-84955569436", "pmid"=>"26352669", "doi"=>"10.1371/journal.pgen.1005500", "pui"=>"607876220", "sgr"=>"84955569436"}, "id"=>"4c613ae9-c3aa-3db5-ba53-28aed44127c1", "abstract"=>"Nature's fastest motors are the cochlear outer hair cells (OHCs). These sensory cells use a membrane protein, Slc26a5 (prestin), to generate mechanical force at high frequencies, which is essential for explaining the exquisite hearing sensitivity of mammalian ears. Previous studies suggest that Slc26a5 continuously diffuses within the membrane, but how can a freely moving motor protein effectively convey forces critical for hearing? To provide direct evidence in OHCs for freely moving Slc26a5 molecules, we created a knockin mouse where Slc26a5 is fused with YFP. These mice and four other strains expressing fluorescently labeled membrane proteins were used to examine their lateral diffusion in the OHC lateral wall. All five proteins showed minimal diffusion, but did move after pharmacological disruption of membrane-associated structures with a cholesterol-depleting agent and salicylate. Thus, our results demonstrate that OHC lateral wall structure constrains the mobility of plasma membrane proteins and that the integrity of such membrane-associated structures are critical for Slc26a5's active and structural roles. The structural constraint of membrane proteins may exemplify convergent evolution of cellular motors across species. Our findings also suggest a possible mechanism for disorders of cholesterol metabolism with hearing loss such as Niemann-Pick Type C diseases.", "link"=>"http://www.mendeley.com/research/outer-hair-cell-lateral-wall-structure-constrains-mobility-plasma-membrane-proteins-1", "reader_count"=>19, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Student > Doctoral Student"=>2, "Researcher"=>2, "Student > Ph. D. Student"=>9, "Other"=>2, "Student > Master"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Student > Doctoral Student"=>2, "Researcher"=>2, "Student > Ph. D. Student"=>9, "Other"=>2, "Student > Master"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>2, "Medicine and Dentistry"=>2, "Agricultural and Biological Sciences"=>9, "Neuroscience"=>3}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Neuroscience"=>{"Neuroscience"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>9}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>2}}, "group_count"=>1}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2256129", "https://ndownloader.figshare.com/files/2256130", "https://ndownloader.figshare.com/files/2256131", "https://ndownloader.figshare.com/files/2256132", "https://ndownloader.figshare.com/files/2256133", "https://ndownloader.figshare.com/files/2256134", "https://ndownloader.figshare.com/files/2256135", "https://ndownloader.figshare.com/files/2256136", "https://ndownloader.figshare.com/files/2256137", "https://ndownloader.figshare.com/files/2256138", "https://ndownloader.figshare.com/files/2256139"], "description"=>"<div><p>Nature’s fastest motors are the cochlear outer hair cells (OHCs). These sensory cells use a membrane protein, Slc26a5 (prestin), to generate mechanical force at high frequencies, which is essential for explaining the exquisite hearing sensitivity of mammalian ears. Previous studies suggest that Slc26a5 continuously diffuses within the membrane, but how can a freely moving motor protein effectively convey forces critical for hearing? To provide direct evidence in OHCs for freely moving Slc26a5 molecules, we created a knockin mouse where Slc26a5 is fused with YFP. These mice and four other strains expressing fluorescently labeled membrane proteins were used to examine their lateral diffusion in the OHC lateral wall. All five proteins showed minimal diffusion, but did move after pharmacological disruption of membrane-associated structures with a cholesterol-depleting agent and salicylate. Thus, our results demonstrate that OHC lateral wall structure constrains the mobility of plasma membrane proteins and that the integrity of such membrane-associated structures are critical for Slc26a5’s active and structural roles. The structural constraint of membrane proteins may exemplify convergent evolution of cellular motors across species. Our findings also suggest a possible mechanism for disorders of cholesterol metabolism with hearing loss such as Niemann-Pick Type C diseases.</p></div>", "links"=>[], "tags"=>["Slc 26a molecules", "SLC 26A", "Membrane proteins", "motor", "ohc", "Outer Hair Cell Lateral Wall Structure Constrains", "yfp", "plasma membrane proteins"], "article_id"=>1538016, "categories"=>["Uncategorised"], "users"=>["Tetsuji Yamashita", "Pierre Hakizimana", "Siva Wu", "Ahmed Hassan", "Stefan Jacob", "Jamshid Temirov", "Jie Fang", "Marcia Mellado-Lagarde", "Richard Gursky", "Linda Horner", "Barbara Leibiger", "Sara Leijon", "Victoria E. Centonze", "Per-Olof Berggren", "Sharon Frase", "Manfred Auer", "William E. Brownell", "Anders Fridberger", "Jian Zuo"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1005500.s001", "https://dx.doi.org/10.1371/journal.pgen.1005500.s002", "https://dx.doi.org/10.1371/journal.pgen.1005500.s003", "https://dx.doi.org/10.1371/journal.pgen.1005500.s004", "https://dx.doi.org/10.1371/journal.pgen.1005500.s005", "https://dx.doi.org/10.1371/journal.pgen.1005500.s006", "https://dx.doi.org/10.1371/journal.pgen.1005500.s007", "https://dx.doi.org/10.1371/journal.pgen.1005500.s008", "https://dx.doi.org/10.1371/journal.pgen.1005500.s009", "https://dx.doi.org/10.1371/journal.pgen.1005500.s010", "https://dx.doi.org/10.1371/journal.pgen.1005500.s011"], "stats"=>{"downloads"=>4, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Outer_Hair_Cell_Lateral_Wall_Structure_Constrains_the_Mobility_of_Plasma_Membrane_Proteins_/1538016", "title"=>"Outer Hair Cell Lateral Wall Structure Constrains the Mobility of Plasma Membrane Proteins", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-09-09 03:18:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/2256065"], "description"=>"<p>FRAP examples using the mice at P18-22 for untreated (A) and PFA-treated (B) OHCs are shown. (C) The normalized fluorescence recovery curves for Slc26a5-YFP based on analysis of the bleached spots (see <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005500#sec013\" target=\"_blank\">Materials and Methods</a>). White arrows (A-B) show bleached spots and the black arrow (C) indicates the time of bleaching. Error bars express S.E.M. Scale bar expresses 10 μm. Numbers (n) of OHCs used in two mice from two litters were shown.</p>", "links"=>[], "tags"=>["Slc 26a molecules", "SLC 26A", "Membrane proteins", "motor", "ohc", "Outer Hair Cell Lateral Wall Structure Constrains", "yfp", "plasma membrane proteins"], "article_id"=>1537965, "categories"=>["Uncategorised"], "users"=>["Tetsuji Yamashita", "Pierre Hakizimana", "Siva Wu", "Ahmed Hassan", "Stefan Jacob", "Jamshid Temirov", "Jie Fang", "Marcia Mellado-Lagarde", "Richard Gursky", "Linda Horner", "Barbara Leibiger", "Sara Leijon", "Victoria E. Centonze", "Per-Olof Berggren", "Sharon Frase", "Manfred Auer", "William E. Brownell", "Anders Fridberger", "Jian Zuo"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005500.g003", "stats"=>{"downloads"=>1, "page_views"=>26, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_FRAP_analysis_reveals_that_Slc26a5_has_little_lateral_mobility_in_the_isolated_Slc26a5_YFP_neo_OHCs_/1537965", "title"=>"FRAP analysis reveals that Slc26a5 has little lateral mobility in the isolated <i>Slc26a5</i><sup><i>YFP/+</i></sup><i>(+neo)</i> OHCs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-09 03:18:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/2256064"], "description"=>"<p>(A-C) NLC using isolated OHCs from <i>Slc26a5</i><sup><i>YFP/+</i></sup><i>(+neo)</i> mice at P20-P26. (A) NLC in isolated OHCs from <i>Slc26a5</i><sup><i>+/+</i></sup> (black dotted lines), <i>Slc26a5</i><sup><i>YFP/+</i></sup><i>(+neo)</i> (red dotted lines), and <i>Slc26a5</i><sup><i>YFP/YFP</i></sup><i>(+neo)</i> (green dotted lines) cochleae is shown. Smooth lines were obtained by fitting to a second order Boltzmann function where population Q<sub>max</sub> and α are shown in (B) and (C) respectively. Black (<i>Slc26a5</i><sup><i>+/+</i></sup>), red (<i>Slc26a5</i><sup><i>YFP/+</i></sup><i>(+neo)</i>), and green (<i>Slc26a5</i><sup><i>YFP/YFP</i></sup><i>(+neo)</i>) bars express the mean (± S.E.M.). Values are the mean ± S.E.M; (D) ABR thresholds of wildtype and Slc26a5-YFP <i>(+neo)</i> mice at P18 are similar (E) ABR thresholds of wildtype and Slc26a5-YFP <i>(-neo)</i> mice at P18 are similar. Values are the mean ± S.E.M.; ***: P<0.001, *: P<0.05 by two-way ANOVA followed by student's t test with a Bonferroni correction.</p>", "links"=>[], "tags"=>["Slc 26a molecules", "SLC 26A", "Membrane proteins", "motor", "ohc", "Outer Hair Cell Lateral Wall Structure Constrains", "yfp", "plasma membrane proteins"], "article_id"=>1537964, "categories"=>["Uncategorised"], "users"=>["Tetsuji Yamashita", "Pierre Hakizimana", "Siva Wu", "Ahmed Hassan", "Stefan Jacob", "Jamshid Temirov", "Jie Fang", "Marcia Mellado-Lagarde", "Richard Gursky", "Linda Horner", "Barbara Leibiger", "Sara Leijon", "Victoria E. Centonze", "Per-Olof Berggren", "Sharon Frase", "Manfred Auer", "William E. Brownell", "Anders Fridberger", "Jian Zuo"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005500.g002", "stats"=>{"downloads"=>4, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Slc26a5_YFP_is_functional_in_Slc26a5_YFP_mice_neo_/1537964", "title"=>"Slc26a5-YFP is functional in Slc26a5-YFP mice <i>(+neo)</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-09 03:18:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/2256080"], "description"=>"<p>(A-D) ChR2-tdTomato heterologously expressed OHCs are shown. Untreated (A), salicylate/MβCD-treated (B), salicylate-treated (C), and MβCD-treated (D) OHCs from <i>Slc26a5-CreER</i><sup><i>T2+/-</i></sup>;<i>ChR2-tdTomato</i><sup><i>f/+</i></sup> mice after tamoxifen was intraperitoneally injected at P6-7 are shown. (E) The normalized fluorescence recovery curves for A-D are shown. (F) ChR2-tdTomato mobility in OHC hair bundles is shown and white-squared areas with hair bundles are shown in the insets. Initial ten data points for ChR2-tdTomato were taken at 0.248 second intervals and the rest of 60 data points were taken at 3 second intervals. (G) The normalized fluorescence recovery curve as for image F is shown. (H) ChR2-tdTomato mobility in Deiters’ cell PM is shown. The inset shows the cell after FRAP obtained by maximum intensity projection of z-stack. Deiters’ cells were isolated from <i>FgfR3-iCreER</i><sup><i>T2+/-</i></sup>;<i>ChR2-tdTomato</i><sup><i>f/+</i></sup> mice intraperitoneally injected with tamoxifen at P6 and P7 (see <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005500#sec013\" target=\"_blank\">Materials and Methods</a>). Each data point was taken at 0.843 second intervals. (I) The normalized fluorescence recovery curve as for image H is shown. Values are the mean ± S.E.M. Scale bars express 10 μm. White arrows (A-D, F, and H) show bleached spots and black arrows indicate the time of bleaching. Numbers (n) of cells analyzed in more than three mice are shown.</p>", "links"=>[], "tags"=>["Slc 26a molecules", "SLC 26A", "Membrane proteins", "motor", "ohc", "Outer Hair Cell Lateral Wall Structure Constrains", "yfp", "plasma membrane proteins"], "article_id"=>1537972, "categories"=>["Uncategorised"], "users"=>["Tetsuji Yamashita", "Pierre Hakizimana", "Siva Wu", "Ahmed Hassan", "Stefan Jacob", "Jamshid Temirov", "Jie Fang", "Marcia Mellado-Lagarde", "Richard Gursky", "Linda Horner", "Barbara Leibiger", "Sara Leijon", "Victoria E. Centonze", "Per-Olof Berggren", "Sharon Frase", "Manfred Auer", "William E. Brownell", "Anders Fridberger", "Jian Zuo"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005500.g008", "stats"=>{"downloads"=>5, "page_views"=>23, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mobility_of_ChR2_tdTomato_in_isolated_OHCs_and_SCs_at_P18_22_using_FRAP_analysis_/1537972", "title"=>"Mobility of ChR2-tdTomato in isolated OHCs and SCs at P18-22 using FRAP analysis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-09 03:18:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/2256077"], "description"=>"<p>TEM studies in untreated (A-E), salicylate-treated (F-J), MβCD-treated (K-O) salicylate/MβCD-treated (P-T) isolated OHCs from the mice at P22-23 are shown. Black squares in A, F, K, P were enlarged in B, G, L, and Q, respectively. Asterisks indicate mitochondria. Arrows indicate assumptive pillars. Scale bar expresses 2 μm in A, F, K, and P and 200 nm in B-E, G-J, L-O and Q-T.</p>", "links"=>[], "tags"=>["Slc 26a molecules", "SLC 26A", "Membrane proteins", "motor", "ohc", "Outer Hair Cell Lateral Wall Structure Constrains", "yfp", "plasma membrane proteins"], "article_id"=>1537969, "categories"=>["Uncategorised"], "users"=>["Tetsuji Yamashita", "Pierre Hakizimana", "Siva Wu", "Ahmed Hassan", "Stefan Jacob", "Jamshid Temirov", "Jie Fang", "Marcia Mellado-Lagarde", "Richard Gursky", "Linda Horner", "Barbara Leibiger", "Sara Leijon", "Victoria E. Centonze", "Per-Olof Berggren", "Sharon Frase", "Manfred Auer", "William E. Brownell", "Anders Fridberger", "Jian Zuo"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005500.g006", "stats"=>{"downloads"=>0, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Ultra_structural_analysis_of_the_OHC_lateral_wall_in_isolated_OHCs_/1537969", "title"=>"Ultra-structural analysis of the OHC lateral wall in isolated OHCs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-09 03:18:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/2256067"], "description"=>"<p>FRAP examples for MβCD-treated (A), latruculin A-treated (B), diamide-treated (C), latrunculin A/ diamide-treated (D), salicylate-treated (E), MβCD/salicylate-treated (F), diamide/latrunculin A/MβCD-treated (G), diamide/latrunculin A/salicylate-treated (H) OHCs from <i>Slc26a5</i><sup><i>YFP/+</i></sup><i>(+neo)</i> mice are shown. Scale bar expresses 10 μm. The dashed line in (F) indicates the position of optical section shown in insets. (I) The normalized fluorescence recovery curves for images A-H are shown (see <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005500#sec013\" target=\"_blank\">Materials and Methods</a>). White arrows in A-H show bleached spots and the black arrow in I indicates the time of bleaching. Error bars express S.E.M. OHC length (J) and Slc26a5 expressing area (K) after each treatment is shown. Values are the mean ± SEM.</p>", "links"=>[], "tags"=>["Slc 26a molecules", "SLC 26A", "Membrane proteins", "motor", "ohc", "Outer Hair Cell Lateral Wall Structure Constrains", "yfp", "plasma membrane proteins"], "article_id"=>1537967, "categories"=>["Uncategorised"], "users"=>["Tetsuji Yamashita", "Pierre Hakizimana", "Siva Wu", "Ahmed Hassan", "Stefan Jacob", "Jamshid Temirov", "Jie Fang", "Marcia Mellado-Lagarde", "Richard Gursky", "Linda Horner", "Barbara Leibiger", "Sara Leijon", "Victoria E. Centonze", "Per-Olof Berggren", "Sharon Frase", "Manfred Auer", "William E. Brownell", "Anders Fridberger", "Jian Zuo"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005500.g005", "stats"=>{"downloads"=>1, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Slc26a5_mobility_analysis_in_the_lateral_wall_of_isolated_OHCs_at_P18_22_/1537967", "title"=>"Slc26a5 mobility analysis in the lateral wall of isolated OHCs at P18-22.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-09 03:18:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/2256066"], "description"=>"<p>(A) YFP fluorescence distribution in a temporal bone preparation from a <i>Slc26a5</i><sup><i>YFP/+</i></sup><i>(+neo)</i> mouse at P38 is shown. (B) Variance of the image series (225 frames) divided with the average brightness (σ<sup>2</sup>/μ) is shown corresponding to A. (C) YFP fluorescence distribution in Min6m9 cells expressing cytosolic YFP (see <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005500#sec013\" target=\"_blank\">Materials and Methods</a>) is shown. The image was taken under an identical condition with (A). B & N analysis for cytosolic YFP in Min6m9 cells (C) is shown in (D). Scale bars express 20 μm.</p>", "links"=>[], "tags"=>["Slc 26a molecules", "SLC 26A", "Membrane proteins", "motor", "ohc", "Outer Hair Cell Lateral Wall Structure Constrains", "yfp", "plasma membrane proteins"], "article_id"=>1537966, "categories"=>["Uncategorised"], "users"=>["Tetsuji Yamashita", "Pierre Hakizimana", "Siva Wu", "Ahmed Hassan", "Stefan Jacob", "Jamshid Temirov", "Jie Fang", "Marcia Mellado-Lagarde", "Richard Gursky", "Linda Horner", "Barbara Leibiger", "Sara Leijon", "Victoria E. Centonze", "Per-Olof Berggren", "Sharon Frase", "Manfred Auer", "William E. Brownell", "Anders Fridberger", "Jian Zuo"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005500.g004", "stats"=>{"downloads"=>2, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_B_N_analysis_confirms_the_minimal_lateral_mobility_of_Slc26a5_YFP_in_Slc26a5_YFP_neo_mice_/1537966", "title"=>"B & N analysis confirms the minimal lateral mobility of Slc26a5-YFP in <i>Slc26a5</i><sup><i>YFP/+</i></sup><i>(+neo)</i> mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-09 03:18:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/2256063"], "description"=>"<p>(A) Schematic illustration shows morphology of OHCs and the OHC lateral wall. The lateral wall of OHCs consists of a unique trilaminate structure composed of PM, actin-spectrin cortical lattice (CL), and SSC. A more detailed model is shown in <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005500#pgen.1005500.s009\" target=\"_blank\">S9 Fig</a>. (B-D) Slc26a5-YFP fluorescence (green) in the apical turn of the cochleae from <i>Slc26a5</i><sup><i>YFP/+</i></sup><i>(+neo)</i> mice at P20. White square in B is enlarged in C. The dashed line in C indicates the position of the optical xz-plane shown in the inset. Myo7a (red) is labeled as a HC marker in D. Counter-staining of nuclei (blue) using DAPI is shown in D. The dashed line in D indicates the position of the optical xz-plane shown in the inset.</p>", "links"=>[], "tags"=>["Slc 26a molecules", "SLC 26A", "Membrane proteins", "motor", "ohc", "Outer Hair Cell Lateral Wall Structure Constrains", "yfp", "plasma membrane proteins"], "article_id"=>1537963, "categories"=>["Uncategorised"], "users"=>["Tetsuji Yamashita", "Pierre Hakizimana", "Siva Wu", "Ahmed Hassan", "Stefan Jacob", "Jamshid Temirov", "Jie Fang", "Marcia Mellado-Lagarde", "Richard Gursky", "Linda Horner", "Barbara Leibiger", "Sara Leijon", "Victoria E. Centonze", "Per-Olof Berggren", "Sharon Frase", "Manfred Auer", "William E. Brownell", "Anders Fridberger", "Jian Zuo"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005500.g001", "stats"=>{"downloads"=>2, "page_views"=>21, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Slc26a5_YFP_recapitulates_endogenous_Slc26a5_distribution_and_is_functional_in_Slc26a5_YFP_neo_mice_/1537963", "title"=>"Slc26a5-YFP recapitulates endogenous Slc26a5 distribution and is functional in <i>Slc26a5</i><sup><i>YFP/+</i></sup><i>(+neo)</i> mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-09 03:18:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/2256078"], "description"=>"<p>Mobile fraction for each molecule was shown either before or after co-treatment of MβCD and salicylate. Molecular weight (M. W. in kDa) was predicted based on amino acid sequences. Means and S.E.M. are expressed.</p>", "links"=>[], "tags"=>["Slc 26a molecules", "SLC 26A", "Membrane proteins", "motor", "ohc", "Outer Hair Cell Lateral Wall Structure Constrains", "yfp", "plasma membrane proteins"], "article_id"=>1537970, "categories"=>["Uncategorised"], "users"=>["Tetsuji Yamashita", "Pierre Hakizimana", "Siva Wu", "Ahmed Hassan", "Stefan Jacob", "Jamshid Temirov", "Jie Fang", "Marcia Mellado-Lagarde", "Richard Gursky", "Linda Horner", "Barbara Leibiger", "Sara Leijon", "Victoria E. Centonze", "Per-Olof Berggren", "Sharon Frase", "Manfred Auer", "William E. Brownell", "Anders Fridberger", "Jian Zuo"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005500.g007", "stats"=>{"downloads"=>1, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Topology_molecular_weight_and_mobile_fractions_before_and_after_treatment_of_Slc26a5_and_four_heterologously_expressed_membrane_proteins_/1537970", "title"=>"Topology, molecular weight, and mobile fractions before and after treatment of Slc26a5 and four heterologously expressed membrane proteins.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-09 03:18:35"}

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  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"6", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"13", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"1", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"12", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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