A Genetic Selection for dinB Mutants Reveals an Interaction between DNA Polymerase IV and the Replicative Polymerase That Is Required for Translesion Synthesis
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{"title"=>"A Genetic Selection for dinB Mutants Reveals an Interaction between DNA Polymerase IV and the Replicative Polymerase That Is Required for Translesion Synthesis", "type"=>"journal", "authors"=>[{"first_name"=>"Michelle K.", "last_name"=>"Scotland", "scopus_author_id"=>"57164766900"}, {"first_name"=>"Justin M.H.", "last_name"=>"Heltzel", "scopus_author_id"=>"26029841700"}, {"first_name"=>"James E.", "last_name"=>"Kath", "scopus_author_id"=>"55387580500"}, {"first_name"=>"Jung Suk", "last_name"=>"Choi", "scopus_author_id"=>"56587051300"}, {"first_name"=>"Anthony J.", "last_name"=>"Berdis", "scopus_author_id"=>"6701840185"}, {"first_name"=>"Joseph J.", "last_name"=>"Loparo", "scopus_author_id"=>"36965089000"}, {"first_name"=>"Mark D.", "last_name"=>"Sutton", "scopus_author_id"=>"7202968104"}], "year"=>2015, "source"=>"PLoS Genetics", "identifiers"=>{"doi"=>"10.1371/journal.pgen.1005507", "issn"=>"15537404", "sgr"=>"84943552904", "pui"=>"606368018", "scopus"=>"2-s2.0-84943552904", "pmid"=>"26352807", "isbn"=>"0030-8870", "arxiv"=>"arXiv:1011.1669v3"}, "id"=>"54720ff7-39d4-3e5d-ab3e-90bf7f75feae", "abstract"=>"Translesion DNA synthesis (TLS) by specialized DNA polymerases (Pols) is a conserved mechanism for tolerating replication blocking DNA lesions. The actions of TLS Pols are managed in part by ring-shaped sliding clamp proteins. In addition to catalyzing TLS, altered expression of TLS Pols impedes cellular growth. The goal of this study was to define the relationship between the physiological function of Escherichia coli Pol IV in TLS and its ability to impede growth when overproduced. To this end, 13 novel Pol IV mutants were identified that failed to impede growth. Subsequent analysis of these mutants suggest that overproduced levels of Pol IV inhibit E. coli growth by gaining inappropriate access to the replication fork via a Pol III-Pol IV switch that is mechanistically similar to that used under physiological conditions to coordinate Pol IV-catalyzed TLS with Pol III-catalyzed replication. Detailed analysis of one mutant, Pol IV-T120P, and two previously described Pol IV mutants impaired for interaction with either the rim (Pol IVR) or the cleft (Pol IVC) of the β sliding clamp revealed novel insights into the mechanism of the Pol III-Pol IV switch. Specifically, Pol IV-T120P retained complete catalytic activity in vitro but, like Pol IVR and Pol IVC, failed to support Pol IV TLS function in vivo. Notably, the T120P mutation abrogated a biochemical interaction of Pol IV with Pol III that was required for Pol III-Pol IV switching. Taken together, these results support a model in which Pol III-Pol IV switching involves interaction of Pol IV with Pol III, as well as the β clamp rim and cleft. Moreover, they provide strong support for the view that Pol III-Pol IV switching represents a vitally important mechanism for regulating TLS in vivo by managing access of Pol IV to the DNA.", "link"=>"http://www.mendeley.com/research/genetic-selection-dinb-mutants-reveals-interaction-between-dna-polymerase-iv-replicative-polymerase", "reader_count"=>18, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Librarian"=>1, "Student > Doctoral Student"=>1, "Researcher"=>2, "Student > Ph. D. Student"=>9, "Other"=>1, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Librarian"=>1, "Student > Doctoral Student"=>1, "Researcher"=>2, "Student > Ph. D. Student"=>9, "Other"=>1, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>9, "Chemistry"=>2, "Computer Science"=>1, "Immunology and Microbiology"=>3}, "reader_count_by_subdiscipline"=>{"Chemistry"=>{"Chemistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>9}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2256048"], "description"=>"<p><i>E</i>. <i>coli</i> strain MS100 was transformed with the indicated plasmids, and aliquots of each transformation reaction were spread onto M9 minimal media supplemented with either glucose or arabinose, as noted. Representative portions of each plate following overnight incubation at 30°C are shown, as well as the ratio of the transformation frequency observed on plates supplemented with arabinose divided by the frequency observed on plates lacking arabinose. Results shown are representative of 2 separate experiments. The steady state level of Pol IV<sup>CD</sup> and Pol IV<sup>CD</sup>-T120P were measured in soluble cell free protein extracts by densitometry of Coomassie Brilliant Blue stained SDS-PAGE following arabinose induction. Based on the density of the region encompassing Pol IV<sup>CD</sup>, minus the background density observed in the pBAD control, wild type Pol IV<sup>CD</sup> (7.06±1.55 density units) and Pol IV<sup>CD</sup>-T120P (7.76±1.09 density units) were present at comparable levels.</p>", "links"=>[], "tags"=>["13 novel Pol IV mutants", "DNA Polymerase IV", "Pol IV mutants", "Pol III", "TLS Pols", "Pol IV", "support Pol IV TLS function", "Escherichia coli Pol IV", "Translesion Synthesis Translesion DNA synthesis", "T 120P mutation", "Pol IVR", "Pol IVC"], "article_id"=>1537952, "categories"=>["Uncategorised"], "users"=>["Michelle K. Scotland", "Justin M. H. Heltzel", "James E. Kath", "Jung-Suk Choi", "Anthony J. Berdis", "Joseph J. Loparo", "Mark D. Sutton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005507.g002", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Ability_of_mutations_in_Pol_IV_CD_to_impede_E_coli_growth_when_overexpressed_/1537952", "title"=>"Ability of mutations in Pol IV<sup>CD</sup> to impede <i>E</i>. <i>coli</i> growth when overexpressed.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-09 03:18:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/2256049"], "description"=>"<p>Replication activity of the indicated Pol IV proteins (red) was measured <b>(A)</b> alone or <b>(B)</b> in the presence of the accessory proteins SSB (blue), β clamp (green) and DnaX using a primer extension assay as described in <i>Materials and Methods</i>.</p>", "links"=>[], "tags"=>["13 novel Pol IV mutants", "DNA Polymerase IV", "Pol IV mutants", "Pol III", "TLS Pols", "Pol IV", "support Pol IV TLS function", "Escherichia coli Pol IV", "Translesion Synthesis Translesion DNA synthesis", "T 120P mutation", "Pol IVR", "Pol IVC"], "article_id"=>1537953, "categories"=>["Uncategorised"], "users"=>["Michelle K. Scotland", "Justin M. H. Heltzel", "James E. Kath", "Jung-Suk Choi", "Anthony J. Berdis", "Joseph J. Loparo", "Mark D. Sutton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005507.g003", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Ability_of_mutant_Pol_IV_proteins_to_catalyze_replication_in_vitro_/1537953", "title"=>"Ability of mutant Pol IV proteins to catalyze replication <i>in vitro</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-09 03:18:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/2256050"], "description"=>"<p><b>(A)</b> The dependence of MMS-induced mutagenesis on Pol IV (<i>dinB</i>) and Pol V (<i>umuDC</i>) function using strains RW118, RW120 (Δ<i>umuDC</i>), VB102 (Δ<i>dinB</i>), and VB103 (Δ<i>dinB</i> Δ<i>umuDC</i>), and <b>(B)</b> the ability of ~4-fold higher than SOS-induced levels of wild type or mutant Pol IV proteins (expressed from a plasmid) to suppress the frequency of Pol V-dependent MMS-induced mutagenesis of strain RW118 by competing with Pol V was measured as described in <i>Materials and Methods</i> and [<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005507#pgen.1005507.ref033\" target=\"_blank\">33</a>]. Results represent the average of at least 4 independent experiments ± one standard deviation. Symbols are as follows: **, <i>p</i><0.0001; *, <i>p</i><0.05.</p>", "links"=>[], "tags"=>["13 novel Pol IV mutants", "DNA Polymerase IV", "Pol IV mutants", "Pol III", "TLS Pols", "Pol IV", "support Pol IV TLS function", "Escherichia coli Pol IV", "Translesion Synthesis Translesion DNA synthesis", "T 120P mutation", "Pol IVR", "Pol IVC"], "article_id"=>1537954, "categories"=>["Uncategorised"], "users"=>["Michelle K. Scotland", "Justin M. H. Heltzel", "James E. Kath", "Jung-Suk Choi", "Anthony J. Berdis", "Joseph J. Loparo", "Mark D. Sutton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005507.g004", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Ability_of_Pol_IV_mutants_to_tolerate_MMS_induced_DNA_damage_in_vivo_/1537954", "title"=>"Ability of Pol IV mutants to tolerate MMS-induced DNA damage <i>in vivo</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-09 03:18:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/2256051"], "description"=>"<p>Cultures of isogenic strains expressing the indicated Pol IV protein from the native <i>dinB</i> locus within the chromosome were serially 10-fold diluted using 0.8% saline, and 10 μl aliquots were spotted onto LB agar plates supplemented with the indicated concentrations of MMS. Results shown are representative of 4 independent experiments. The <i>zaf-3633</i>::<i>cat</i> allele, which is linked to the <i>dinB</i> locus and was used in strain construction, does not affect MMS sensitivity of the strains.</p>", "links"=>[], "tags"=>["13 novel Pol IV mutants", "DNA Polymerase IV", "Pol IV mutants", "Pol III", "TLS Pols", "Pol IV", "support Pol IV TLS function", "Escherichia coli Pol IV", "Translesion Synthesis Translesion DNA synthesis", "T 120P mutation", "Pol IVR", "Pol IVC"], "article_id"=>1537955, "categories"=>["Uncategorised"], "users"=>["Michelle K. Scotland", "Justin M. H. Heltzel", "James E. Kath", "Jung-Suk Choi", "Anthony J. Berdis", "Joseph J. Loparo", "Mark D. Sutton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005507.g005", "stats"=>{"downloads"=>10, "page_views"=>22, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_Pol_IV_T120P_Pol_IV_C_and_Pol_IV_R_strains_are_sensitized_to_killing_by_MMS_/1537955", "title"=>"The Pol IV-T120P, Pol IV<sup>C</sup> and Pol IV<sup>R</sup> strains are sensitized to killing by MMS.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-09 03:18:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/2256052"], "description"=>"<p><b>(A)</b> The dependence of MMS-induced mutagenesis on Pol IV (<i>dinB</i>) and Pol V (<i>umuDC</i>) function using strains MG1655, MKS101 (Δ<i>umuDC</i>), MKS100 (Δ<i>dinB</i>), and MKS102 (Δ<i>dinB</i> Δ<i>umuDC</i>). <b>(B)</b> The ability of wild type or mutant Pol IV proteins to suppress the frequency of Pol V-dependent MMS-induced mutagenesis when expressed from the chromosome was measured as described in <i>Materials and Methods</i> [<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005507#pgen.1005507.ref033\" target=\"_blank\">33</a>]. Results represent the average of at least 4 independent experiments ± one standard deviation. Symbols are as follows: **, <i>p</i><0.0001; *, <i>p</i><0.05.</p>", "links"=>[], "tags"=>["13 novel Pol IV mutants", "DNA Polymerase IV", "Pol IV mutants", "Pol III", "TLS Pols", "Pol IV", "support Pol IV TLS function", "Escherichia coli Pol IV", "Translesion Synthesis Translesion DNA synthesis", "T 120P mutation", "Pol IVR", "Pol IVC"], "article_id"=>1537956, "categories"=>["Uncategorised"], "users"=>["Michelle K. Scotland", "Justin M. H. Heltzel", "James E. Kath", "Jung-Suk Choi", "Anthony J. Berdis", "Joseph J. Loparo", "Mark D. Sutton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005507.g006", "stats"=>{"downloads"=>7, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_Pol_IV_T120P_Pol_IV_C_and_Pol_IV_R_strains_display_an_increased_frequency_of_MMS_induced_mutagenesis_/1537956", "title"=>"The Pol IV-T120P, Pol IV<sup>C</sup> and Pol IV<sup>R</sup> strains display an increased frequency of MMS-induced mutagenesis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-09 03:18:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/2256053"], "description"=>"<p><b>(A)</b> Representative trajectories for primer extension of individual DNA molecules by Pol III alone (5 nM), or in the presence of excess Pol IV (300 nM). Processive events are marked in blue (Pol III) or red (Pol IV), with intervening pauses in grey. <b>(B)</b> A reduction in Pol III processivity in the presence of excess Pol IV (circles) can be abrogated by removing the Pol IV CBM (Pol IV<sup>C</sup>, triangle), but not by the T120P mutation (square, note overlap). Each point represents the mean of 50–470 processive events ± the standard error of the mean. <b>(C)</b> The catalytic domain of Pol IV (Pol IV<sup>CD</sup>, 900 nM) disrupts active Pol III (5 nM) synthesis, while an equivalent concentration of Pol IV<sup>CD</sup>-T120P does not. Bars represent the mean of 470, 301 and 657 separate processive events, respectively, ± the standard error of the mean.</p>", "links"=>[], "tags"=>["13 novel Pol IV mutants", "DNA Polymerase IV", "Pol IV mutants", "Pol III", "TLS Pols", "Pol IV", "support Pol IV TLS function", "Escherichia coli Pol IV", "Translesion Synthesis Translesion DNA synthesis", "T 120P mutation", "Pol IVR", "Pol IVC"], "article_id"=>1537957, "categories"=>["Uncategorised"], "users"=>["Michelle K. Scotland", "Justin M. H. Heltzel", "James E. Kath", "Jung-Suk Choi", "Anthony J. Berdis", "Joseph J. Loparo", "Mark D. Sutton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005507.g007", "stats"=>{"downloads"=>1, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Pol_IV_CD_T120P_but_not_full_length_Pol_IV_T120P_fails_to_inhibit_Pol_III_processivity_in_vitro_/1537957", "title"=>"Pol IV<sup>CD</sup>-T120P, but not full length Pol IV-T120P, fails to inhibit Pol III processivity <i>in vitro</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-09 03:18:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/2256054"], "description"=>"<p>Models for the structure of the Pol IIIαεθ-β clamp-DNA complex with Pol IV bound to the rim of the β clamp that is immediately adjacent to the β cleft that is bound by either <b>(A)</b> Pol IIIα (red) or <b>(B)</b> Pol IIIε (blue). These models were built using MacPyMol Molecular Graphics System, Version 1.7.4 Schrödinger, LLC and the previously published model for the Pol IIIαεθ-β clamp-DNA complex [<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005507#pgen.1005507.ref084\" target=\"_blank\">84</a>]. Pol IV (PDB 4IR9) was docked onto the rim of the β clamp in complex with Pol IIIαεθ by aligning it with the Pol IV<sup>LF</sup> domain in PDB 1UNN [<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005507#pgen.1005507.ref012\" target=\"_blank\">12</a>,<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005507#pgen.1005507.ref053\" target=\"_blank\">53</a>]. Residue T120 of Pol IV is in orange, while α-helix 5 corresponding to residues H116-Q135 of Pol IV is in yellow. <b>(C)</b> Model of the Pol III-Pol IV switching mechanism in which Pol IV (cyan) gains access to the DNA template after making contact with one or more subunits of Pol III (green), as well as the rim of the β clamp (purple). The small black circles represent the clefts in the β clamp. See text for further details.</p>", "links"=>[], "tags"=>["13 novel Pol IV mutants", "DNA Polymerase IV", "Pol IV mutants", "Pol III", "TLS Pols", "Pol IV", "support Pol IV TLS function", "Escherichia coli Pol IV", "Translesion Synthesis Translesion DNA synthesis", "T 120P mutation", "Pol IVR", "Pol IVC"], "article_id"=>1537958, "categories"=>["Uncategorised"], "users"=>["Michelle K. Scotland", "Justin M. H. Heltzel", "James E. Kath", "Jung-Suk Choi", "Anthony J. Berdis", "Joseph J. Loparo", "Mark D. Sutton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005507.g008", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Models_for_the_role_of_residue_T120_in_Pol_IV_function_/1537958", "title"=>"Models for the role of residue T120 in Pol IV function.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-09 03:18:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/2256055"], "description"=>"<p><sup><b><i>a</i></b></sup> See <i><a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005507#sec010\" target=\"_blank\">Materials and Methods</a></i> for a description of how strains and plasmid DNAs were constructed and verified.</p><p>NEB, New England Biolabs; CGSC, <i>E</i>. <i>coli</i> Genetic Stock Center, Yale University.</p><p>Bacterial strains and plasmid DNAs used in this study.</p>", "links"=>[], "tags"=>["13 novel Pol IV mutants", "DNA Polymerase IV", "Pol IV mutants", "Pol III", "TLS Pols", "Pol IV", "support Pol IV TLS function", "Escherichia coli Pol IV", "Translesion Synthesis Translesion DNA synthesis", "T 120P mutation", "Pol IVR", "Pol IVC"], "article_id"=>1537959, "categories"=>["Uncategorised"], "users"=>["Michelle K. Scotland", "Justin M. H. Heltzel", "James E. Kath", "Jung-Suk Choi", "Anthony J. Berdis", "Joseph J. Loparo", "Mark D. Sutton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005507.t001", "stats"=>{"downloads"=>6, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Bacterial_strains_and_plasmid_DNAs_used_in_this_study_/1537959", "title"=>"Bacterial strains and plasmid DNAs used in this study.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-09-09 03:18:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/2256056"], "description"=>"<p><sup><b><i>a</i></b></sup> Nucleotide changes are represented in the context of the codon in which they reside. Numbers refer to the first and last base in the codon, with position 1 representing the A in the ATG encoding the first amino acid of Pol IV (<i>i</i>.<i>e</i>., <sup>1</sup>ATG<sup>3</sup>).</p><p><sup><b><i>b</i></b></sup> Allele numbers were assigned by the <i>E</i>. <i>coli</i> Genetic Stock Center (CGSC) at Yale.</p><p><sup><b><i>c</i></b></sup> The number of times that each mutation was identified is indicated.</p><p><sup><b><i>d</i></b></sup> Proposed functions for Pol IV residues are based largely on structural insights from Bunting <i>et al</i>. [<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005507#pgen.1005507.ref032\" target=\"_blank\">32</a>], Sharma <i>et al</i>. [<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005507#pgen.1005507.ref053\" target=\"_blank\">53</a>], Boudsocq <i>et al</i>. [<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005507#pgen.1005507.ref054\" target=\"_blank\">54</a>] and Ling <i>et al</i>. [<a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005507#pgen.1005507.ref055\" target=\"_blank\">55</a>].</p><p><sup><b><i>e</i></b></sup> When expressed at high levels from a T7 promoter, recombinant Pol IV mutant proteins were either soluble (+), or poorly soluble and/or extensively proteolyzed (–), as indicated.</p><p><sup><b><i>f</i></b></sup> One of the three A44D clones identified contained an additional silent mutation at I74 (<sup>220</sup>ATC<sup>222</sup>→<sup>220</sup>ATT<sup>222</sup>). This allele was not further characterized.</p><p><sup><b><i>g</i></b></sup> The <sup>937</sup>GGG<sup>939</sup>→GGC substitution present in plasmid pJH154 represents a silent mutation at position G313.</p><p><sup><b><i>h</i></b></sup> The Pol IV-R75L mutant was expressed as a soluble full-length protein from the T7 promoter, but was inconsistently detected following expression in Pol IV<sup>CD</sup> from the arabinose promoter of plasmid pDB22, suggesting Pol IV<sup>CD</sup>-R75L was misfolded.</p><p>Summary of novel <i>dinB</i> alleles.</p>", "links"=>[], "tags"=>["13 novel Pol IV mutants", "DNA Polymerase IV", "Pol IV mutants", "Pol III", "TLS Pols", "Pol IV", "support Pol IV TLS function", "Escherichia coli Pol IV", "Translesion Synthesis Translesion DNA synthesis", "T 120P mutation", "Pol IVR", "Pol IVC"], "article_id"=>1537960, "categories"=>["Uncategorised"], "users"=>["Michelle K. Scotland", "Justin M. H. Heltzel", "James E. Kath", "Jung-Suk Choi", "Anthony J. Berdis", "Joseph J. Loparo", "Mark D. Sutton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005507.t002", "stats"=>{"downloads"=>1, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Summary_of_novel_dinB_alleles_/1537960", "title"=>"Summary of novel <i>dinB</i> alleles.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-09-09 03:18:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/2256057"], "description"=>"<p><sup><b><i>a</i></b></sup> The complete nucleotide sequence of each oligonucleotide is provided in <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005507#pgen.1005507.s009\" target=\"_blank\">S3 Table</a>.</p><p><sup><b><i>b</i></b></sup><i>ND</i>, not determined; accurate kinetic parameters could not be measured due to poor incorporation efficiencies at the highest dNTP concentration tested (500 μM).</p><p>Kinetic constants for wild type Pol IV and Pol IV-T120P nucleotide incorporation.</p>", "links"=>[], "tags"=>["13 novel Pol IV mutants", "DNA Polymerase IV", "Pol IV mutants", "Pol III", "TLS Pols", "Pol IV", "support Pol IV TLS function", "Escherichia coli Pol IV", "Translesion Synthesis Translesion DNA synthesis", "T 120P mutation", "Pol IVR", "Pol IVC"], "article_id"=>1537961, "categories"=>["Uncategorised"], "users"=>["Michelle K. Scotland", "Justin M. H. Heltzel", "James E. Kath", "Jung-Suk Choi", "Anthony J. Berdis", "Joseph J. Loparo", "Mark D. Sutton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005507.t003", "stats"=>{"downloads"=>5, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Kinetic_constants_for_wild_type_Pol_IV_and_Pol_IV_T120P_nucleotide_incorporation_/1537961", "title"=>"Kinetic constants for wild type Pol IV and Pol IV-T120P nucleotide incorporation.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-09-09 03:18:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/2256068", "https://ndownloader.figshare.com/files/2256069", "https://ndownloader.figshare.com/files/2256070", "https://ndownloader.figshare.com/files/2256071", "https://ndownloader.figshare.com/files/2256072", "https://ndownloader.figshare.com/files/2256073", "https://ndownloader.figshare.com/files/2256074", "https://ndownloader.figshare.com/files/2256075", "https://ndownloader.figshare.com/files/2256076"], "description"=>"<div><p>Translesion DNA synthesis (TLS) by specialized DNA polymerases (Pols) is a conserved mechanism for tolerating replication blocking DNA lesions. The actions of TLS Pols are managed in part by ring-shaped sliding clamp proteins. In addition to catalyzing TLS, altered expression of TLS Pols impedes cellular growth. The goal of this study was to define the relationship between the physiological function of <i>Escherichia coli</i> Pol IV in TLS and its ability to impede growth when overproduced. To this end, 13 novel Pol IV mutants were identified that failed to impede growth. Subsequent analysis of these mutants suggest that overproduced levels of Pol IV inhibit <i>E</i>. <i>coli</i> growth by gaining inappropriate access to the replication fork <i>via</i> a Pol III-Pol IV switch that is mechanistically similar to that used under physiological conditions to coordinate Pol IV-catalyzed TLS with Pol III-catalyzed replication. Detailed analysis of one mutant, Pol IV-T120P, and two previously described Pol IV mutants impaired for interaction with either the rim (Pol IV<sup>R</sup>) or the cleft (Pol IV<sup>C</sup>) of the β sliding clamp revealed novel insights into the mechanism of the Pol III-Pol IV switch. Specifically, Pol IV-T120P retained complete catalytic activity <i>in vitro</i> but, like Pol IV<sup>R</sup> and Pol IV<sup>C</sup>, failed to support Pol IV TLS function <i>in vivo</i>. Notably, the T120P mutation abrogated a biochemical interaction of Pol IV with Pol III that was required for Pol III-Pol IV switching. Taken together, these results support a model in which Pol III-Pol IV switching involves interaction of Pol IV with Pol III, as well as the β clamp rim and cleft. Moreover, they provide strong support for the view that Pol III-Pol IV switching represents a vitally important mechanism for regulating TLS <i>in vivo</i> by managing access of Pol IV to the DNA.</p></div>", "links"=>[], "tags"=>["13 novel Pol IV mutants", "DNA Polymerase IV", "Pol IV mutants", "Pol III", "TLS Pols", "Pol IV", "support Pol IV TLS function", "Escherichia coli Pol IV", "Translesion Synthesis Translesion DNA synthesis", "T 120P mutation", "Pol IVR", "Pol IVC"], "article_id"=>1537968, "categories"=>["Uncategorised"], "users"=>["Michelle K. Scotland", "Justin M. H. Heltzel", "James E. Kath", "Jung-Suk Choi", "Anthony J. Berdis", "Joseph J. Loparo", "Mark D. Sutton"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1005507.s001", "https://dx.doi.org/10.1371/journal.pgen.1005507.s002", "https://dx.doi.org/10.1371/journal.pgen.1005507.s003", "https://dx.doi.org/10.1371/journal.pgen.1005507.s004", "https://dx.doi.org/10.1371/journal.pgen.1005507.s005", "https://dx.doi.org/10.1371/journal.pgen.1005507.s006", "https://dx.doi.org/10.1371/journal.pgen.1005507.s007", "https://dx.doi.org/10.1371/journal.pgen.1005507.s008", "https://dx.doi.org/10.1371/journal.pgen.1005507.s009"], "stats"=>{"downloads"=>10, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/A_Genetic_Selection_for_dinB_Mutants_Reveals_an_Interaction_between_DNA_Polymerase_IV_and_the_Replicative_Polymerase_That_Is_Required_for_Translesion_Synthesis/1537968", "title"=>"A Genetic Selection for <i>dinB</i> Mutants Reveals an Interaction between DNA Polymerase IV and the Replicative Polymerase That Is Required for Translesion Synthesis", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-09-09 03:18:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/2256043"], "description"=>"<p><b>(A)</b> Positions of the mutations identified in this work are represented on a linear model of Pol IV. Each domain of Pol IV is color-coded: palm domain (residues 1–10 and 74–165), magenta; fingers domain (residues 11–73), cyan; thumb domain (residues 166–240), blue; little finger domain (residues 243–351), orange. Residues involved in Pol IV catalytic activity (D8, D103, and E104), and β clamp rim (<sup>303</sup>VWP<sup>305</sup>) and β cleft (<sup>346</sup>QLVLGL<sup>351</sup>) interactions are highlighted on the bottom of the linear diagram. Pymol models of β clamp assembled on DNA and bound to Pol IV in either a <b>(B)</b> non-replicative or <b>(C)</b> replicative mode. In the non-replicative mode, Pol IV<sup>LF</sup> contacts both the rim and the cleft of the β clamp. Interaction of the Pol IV<sup>LF</sup> with the β clamp rim acts to pull Pol IV to the side of the β clamp and away from the DNA template. In the replicative mode, Pol IV is bound to the β clamp cleft and the DNA template. Since it is no longer associated with the β clamp rim, Pol IV sits on the face of the β clamp and can now access the DNA template. These models were built using MacPyMol Molecular Graphics System, Version 1.7.4 Schrödinger, LLC and coordinates for the Pol IV-DNA (PDB 4IR9) and Pol IV<sup>LF</sup> domain in complex with β clamp (PDB 1UNN). For the model depicting the non-replicative mode of binding, the Pol IV<sup>LF</sup> domain of Pol IV in the Pol IV-DNA structure was aligned with the Pol IV<sup>LF</sup> domain in the Pol IV<sup>LF</sup>-β clamp complex. For the replicative mode, Pol IV was rotated to the face of the β clamp by aligning it with the DNA template passing though the center of β clamp while maintaining the interaction of Pol IV<sup>LF</sup> with the β clamp cleft. Once full length Pol IV was aligned, the Pol IV<sup>LF</sup> structure in 1UNN was hidden from view. 4IR9 does not include residues 342–351; hence residues 342–351 in 1UNN were left visible to complete the structure of Pol IV in both models. <b>(D)</b> The position of residue D8 relative to D10, D103 and E104, which comprise the catalytic center of Pol IV, is shown, as are <b>(E)</b> residues R75 and D20, which may form a hydrogen bond between the palm and the fingers domain, helping to stabilize the tertiary structure of Pol IV, and residues <b>(F)</b> G183, <b>(G)</b> G219 and <b>(H)</b> G323, which may contact the DNA template.</p>", "links"=>[], "tags"=>["13 novel Pol IV mutants", "DNA Polymerase IV", "Pol IV mutants", "Pol III", "TLS Pols", "Pol IV", "support Pol IV TLS function", "Escherichia coli Pol IV", "Translesion Synthesis Translesion DNA synthesis", "T 120P mutation", "Pol IVR", "Pol IVC"], "article_id"=>1537947, "categories"=>["Uncategorised"], "users"=>["Michelle K. Scotland", "Justin M. H. Heltzel", "James E. Kath", "Jung-Suk Choi", "Anthony J. Berdis", "Joseph J. Loparo", "Mark D. Sutton"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005507.g001", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Positions_of_mutations_represented_on_structural_models_for_the_Pol_IV_946_clamp_DNA_complex_/1537947", "title"=>"Positions of mutations represented on structural models for the Pol IV-β clamp-DNA complex.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-09 03:18:19"}

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Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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