Basolateral Endocytic Recycling Requires RAB-10 and AMPH-1 Mediated Recruitment of RAB-5 GAP TBC-2 to Endosomes
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Basolateral Endocytic Recycling Requires RAB-10 and AMPH-1 Mediated Recruitment of RAB-5 GAP TBC-2 to Endosomes", "type"=>"journal", "authors"=>[{"first_name"=>"Ou", "last_name"=>"Liu", "scopus_author_id"=>"55328216300"}, {"first_name"=>"Barth D.", "last_name"=>"Grant", "scopus_author_id"=>"25821900100"}], "year"=>2015, "source"=>"PLoS Genetics", "identifiers"=>{"pmid"=>"26393361", "isbn"=>"1553-7390", "doi"=>"10.1371/journal.pgen.1005514", "issn"=>"15537404", "scopus"=>"2-s2.0-84943572023", "pui"=>"606367653", "sgr"=>"84943572023"}, "id"=>"402ae8d3-7da8-3c05-b401-10cd4d34ebfe", "abstract"=>"The small GTPase RAB-5/Rab5 is a master regulator of the early endosome, required for a myriad of coordinated activities, including the degradation and recycling of internalized cargo. Here we focused on the recycling function of the early endosome and the regulation of RAB-5 by GAP protein TBC-2 in the basolateral C. elegans intestine. We demonstrate that downstream basolateral recycling regulators, GTPase RAB-10/Rab10 and BAR domain protein AMPH-1/Amphiphysin, bind to TBC-2 and help to recruit it to endosomes. In the absence of RAB-10 or AMPH-1 binding to TBC-2, RAB-5 membrane association is abnormally high and recycling cargo is trapped in early endosomes. Furthermore, the loss of TBC-2 or AMPH-1 leads to abnormally high spatial overlap of RAB-5 and RAB-10. Taken together our results indicate that RAB-10 and AMPH-1 mediated down-regulation of RAB-5 is an important step in recycling, required for cargo exit from early endosomes and regulation of early endosome-recycling endosome interactions.", "link"=>"http://www.mendeley.com/research/basolateral-endocytic-recycling-requires-rab10-amph1-mediated-recruitment-rab5-gap-tbc2-endosomes", "reader_count"=>17, "reader_count_by_academic_status"=>{"Researcher"=>2, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>1, "Student > Master"=>1, "Student > Bachelor"=>6, "Professor"=>1}, "reader_count_by_user_role"=>{"Researcher"=>2, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>1, "Student > Master"=>1, "Student > Bachelor"=>6, "Professor"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>4, "Agricultural and Biological Sciences"=>10, "Medicine and Dentistry"=>3}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>10}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84943572023"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84943572023?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84943572023&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84943572023&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84943572023", "dc:identifier"=>"SCOPUS_ID:84943572023", "eid"=>"2-s2.0-84943572023", "dc:title"=>"Basolateral Endocytic Recycling Requires RAB-10 and AMPH-1 Mediated Recruitment of RAB-5 GAP TBC-2 to Endosomes", "dc:creator"=>"Liu O.", "prism:publicationName"=>"PLoS Genetics", "prism:issn"=>"15537390", "prism:eIssn"=>"15537404", "prism:volume"=>"11", "prism:issueIdentifier"=>"9", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-01-01", "prism:coverDisplayDate"=>"2015", "prism:doi"=>"10.1371/journal.pgen.1005514", "citedby-count"=>"11", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Rutgers University–New Brunswick", "affiliation-city"=>"New Brunswick", "affiliation-country"=>"United States"}], "pubmed-id"=>"26393361", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e1005514", "source-id"=>"4000151808", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosgenetics%2Farticle%3Fid%3D10.1371%252Fjournal.pgen.1005514", "share_count"=>1, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>1}

Counter

  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"70", "xml_views"=>"2", "html_views"=>"466"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"54", "xml_views"=>"5", "html_views"=>"301"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"69"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"69"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"84"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"59", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"60", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"34"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"2", "html_views"=>"13"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"18", "xml_views"=>"5", "html_views"=>"18"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"10"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"3", "html_views"=>"14"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"19"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"35", "xml_views"=>"2", "html_views"=>"48"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"5"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2284013"], "description"=>"<p>All images are from deconvolved 3D confocal image stacks acquired in intact living animals expressing GFP- and RFP-tagged proteins specifically in intestinal epithelial cells. (A-A‴) Under wild-type conditions, tagRFP-RAB-5 and GFP-RAB-10 display partial colocalization (~53%) on punctate endosomal structures. (B-B‴) In <i>tbc-2 (tm2241)</i> mutant animals, tagRFP-RAB-5 and GFP-RAB-10 label enlarged pleiomorphic structures and their degree of overlap increased to ~71%. (C-C‴) <i>amph-1</i> mutants also displayed increased overlap between tagRFP-RAB-5 and GFP-RAB-10 (~76%) with less severe morphological change than that caused by <i>tbc-2</i> mutants. In each image autofluorescent lysosome-like organelles appears in blue in all three channels, whereas GFP appears only in the green channel and RFP shows up only in the red channel. Signals observed in the green or red channels that do not overlap with signals in the blue channel are considered bona fide GFP or RFP signals respectively. (Scale bar: 10 μm) (D) Pearson's correlation coefficient for colocalization of tagRFP-RAB-5 and GFP-RAB-10. n = 6. Error bars represent SEM. **P<0.01, ***P<0.001(student's t test).</p>", "links"=>[], "tags"=>["endosome", "elegans intestine", "Membrane Association", "recycling function", "rab", "master regulator", "gap", "cargo exit", "basolateral C", "recycling cargo", "basolateral recycling regulators", "amph", "Mediated Recruitment", "tbc"], "article_id"=>1552725, "categories"=>["Uncategorised"], "users"=>["Ou Liu", "Barth D. Grant"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005514.g006", "stats"=>{"downloads"=>3, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Loss_of_TBC_2_or_AMPH_1_alters_the_spatial_coordination_of_RAB_5_and_RAB_10_/1552725", "title"=>"Loss of TBC-2 or AMPH-1 alters the spatial coordination of RAB-5 and RAB-10.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-22 03:41:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/2284007"], "description"=>"<p>All images were collected from living intact adult animals expressing intestine-specific transgenes. Representative confocal images of GFP-TBC-2 in wild-type (A), <i>rab-10(ok1494)</i> mutant (B), and <i>amph-1(tm1060)</i> mutant (C) backgrounds are shown. The endosomal localization of GFP-TBC-2 is strongly reduced in <i>rab-10(ok1494)</i> and <i>amph-1(tm1060)</i> mutant backgrounds. (Scale bar: 10 μm) (D) Quantification of the average total intensity of GFP-TBC-2 labeled structures. (n = 18 each, 6 animals of each genotype sampled in three different regions of each intestine.) Error bars represent SEM. ***P<0.001(student's t test). (E) Western Blot analysis of young adult worms expressing GFP-TBC-2 in wild-type, <i>rab-10(ok1494)</i> mutant, and <i>amph-1(tm1060)</i> mutant backgrounds. Blots with anti-GFP and anti-actin antibodies are shown.</p>", "links"=>[], "tags"=>["endosome", "elegans intestine", "Membrane Association", "recycling function", "rab", "master regulator", "gap", "cargo exit", "basolateral C", "recycling cargo", "basolateral recycling regulators", "amph", "Mediated Recruitment", "tbc"], "article_id"=>1552721, "categories"=>["Uncategorised"], "users"=>["Ou Liu", "Barth D. Grant"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005514.g003", "stats"=>{"downloads"=>2, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_RAB_10_and_AMPH_1_contribute_to_the_endosome_recruitment_of_TBC_2_/1552721", "title"=>"RAB-10 and AMPH-1 contribute to the endosome recruitment of TBC-2.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-22 03:41:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/2284005"], "description"=>"<p>(A) and (B) The interaction between TBC-2 and RAB-10(Q68L) requires a segment of TBC-2(AA 279–321) containing a predicted coiled-coil domain. RAB-10(Q68L) was expressed in a yeast reporter strain as a fusion to the DNA-binding domain of LexA (bait). Different truncations of TBC-2 were expressed in the same yeast cells as fusions to the B42 transcriptional activation domain (prey). Interaction between bait and prey was assayed by complementation of leucine auxotrophy (LEU2 growth assay). Colonies were diluted in liquid and spotted on solid growth medium directly (1X) or after further dilution (0.1X). (C) Alanine substitutions within the critical RAB-10-binding sequence of TBC-2 (positions aa283-287, aa288-292, aa294-298) abolished the interaction between TBC-2 and RAB-10(Q68L). (D) Full-length TBC-2 interacts with AMPH-1. Mutation of key residues (prolines P150 or P153, or arginine R155) within TBC-2 (aa146-160), the predicted consensus sequence for AMPH-1 SH3-domain-binding, into alanine via site-directed mutagenesis disrupted the interaction between TBC-2 and the SH3 domain of AMPH-1. AMPH-1 SH3 domain was expressed as bait. Full-length and mutated forms of TBC-2 were expressed as prey. Their interactions were detected using the same two-hybrid assay as described in (A) and (B). (E) Schematic representation of TBC-2 domains and the truncated fragments of TBC-2 used in the Y2H analysis. Protein domains are displayed as dark boxes above the protein sequences (represented by dark lines). Amino acid numbers are indicated.</p>", "links"=>[], "tags"=>["endosome", "elegans intestine", "Membrane Association", "recycling function", "rab", "master regulator", "gap", "cargo exit", "basolateral C", "recycling cargo", "basolateral recycling regulators", "amph", "Mediated Recruitment", "tbc"], "article_id"=>1552719, "categories"=>["Uncategorised"], "users"=>["Ou Liu", "Barth D. Grant"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005514.g001", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_TBC_2_interacts_physically_with_RAB_10_and_AMPH_1_/1552719", "title"=>"TBC-2 interacts physically with RAB-10 and AMPH-1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-22 03:41:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/2284006"], "description"=>"<p>All images are from deconvolved 3D confocal image stacks acquired in intact living animals expressing GFP- and RFP-tagged proteins specifically in intestinal epithelial cells. (A-A'') GFP-TBC-2 colocalizes well with tagRFP-RAB-10. Arrowheads indicate endosomes labeled by both GFP-TBC-2 and tagRFP-RAB-10. (A‴) Magnified image of A'' is designated by rectangular outline. (B-B'') GFP-TBC-2 colocalizes extensively with tagRFP-RAB-10(Q68L), a predicted constitutively active form of RAB-10. Arrowheads indicate endosomes labeled by both GFP-TBC-2 and tagRFP-RAB-10(Q68L). (B‴) Magnified image of B'' is designated by rectangular outline. (C-C'') GFP-TBC-2 colocalizes well with CNT-1-MC. Arrowheads indicate endosomes labeled by both GFP-TBC-2 and CNT-1-MC. (C‴) Magnified image of C'' is designated by rectangular outline. In each image autofluorescent lysosome-like organelles appear in blue in all three channels, whereas GFP appears only in the green channel and RFP shows up only in the red channel. Signals observed in the green or red channels that do not overlap with signals in the blue channel are considered bona fide GFP or RFP signals respectively. (Scale bar: 10 μm) (D) Pearson's correlation coefficient for colocalization of GFP-TBC-2 with tagRFP-RAB-10, tagRFP-RAB-10(Q68L), and CNT-1-MC. n = 6. Error bars represent SEM. *P<0.05(student's t test).</p>", "links"=>[], "tags"=>["endosome", "elegans intestine", "Membrane Association", "recycling function", "rab", "master regulator", "gap", "cargo exit", "basolateral C", "recycling cargo", "basolateral recycling regulators", "amph", "Mediated Recruitment", "tbc"], "article_id"=>1552720, "categories"=>["Uncategorised"], "users"=>["Ou Liu", "Barth D. Grant"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005514.g002", "stats"=>{"downloads"=>3, "page_views"=>125, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_TBC_2_colocalizes_with_RAB_10_and_CNT_1_on_endosomes_/1552720", "title"=>"TBC-2 colocalizes with RAB-10 and CNT-1 on endosomes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-22 03:41:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/2284027"], "description"=>"<p>All images are from deconvolved 3D confocal image stacks acquired in intact living animals expressing a GFP-tagged recycling cargo protein, the IL-2 receptor alpha-chain (hTAC-GFP) and tagRFP-RAB-10 in wild-type animals (A-A‴), <i>tbc-2 (tm2241)</i> (B-B‴) and <i>amph-1(tm1060)</i> mutants (C-C‴). (A-A‴) In wild-type animals, hTAC-GFP and tagRFP-RAB-10 colocalizes partially. (B-B‴) In <i>tbc-2</i> mutants, the degree of colocalization between hTAC-GFP and tagRFP-RAB-10 increased and they mainly overlap on punctate endosomal structures. (C-C‴) In <i>amph-1</i> mutants, no obvious change in the degree of overlap between hTAC-GFP and tagRFP-RAB-10 was detected in comparison to that in wild-type animals. In each image autofluorescent lysosome-like organelles appears in blue in all three channels, whereas GFP appears only in the green channel and RFP shows up only in the red channel. Signals observed in the green or red channels that do not overlap with signals in the blue channel are considered bona fide GFP or RFP signals respectively. (Scale bar: 10 μm) (D) Pearson's correlation coefficient for colocalization of hTAC-GFP and tagRFP-RAB-10. n = 6. Error bars represent SEM. **P<0.01(student's t test).</p>", "links"=>[], "tags"=>["endosome", "elegans intestine", "Membrane Association", "recycling function", "rab", "master regulator", "gap", "cargo exit", "basolateral C", "recycling cargo", "basolateral recycling regulators", "amph", "Mediated Recruitment", "tbc"], "article_id"=>1552727, "categories"=>["Uncategorised"], "users"=>["Ou Liu", "Barth D. Grant"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005514.g008", "stats"=>{"downloads"=>5, "page_views"=>62, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Colocalization_of_recycling_cargo_on_RAB_10_labeled_endosomes_/1552727", "title"=>"Colocalization of recycling cargo on RAB-10 labeled endosomes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-22 03:41:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/2284028", "https://ndownloader.figshare.com/files/2284029", "https://ndownloader.figshare.com/files/2284030", "https://ndownloader.figshare.com/files/2284031", "https://ndownloader.figshare.com/files/2284032", "https://ndownloader.figshare.com/files/2284033"], "description"=>"<div><p>The small GTPase RAB-5/Rab5 is a master regulator of the early endosome, required for a myriad of coordinated activities, including the degradation and recycling of internalized cargo. Here we focused on the recycling function of the early endosome and the regulation of RAB-5 by GAP protein TBC-2 in the basolateral <i>C</i>. <i>elegans</i> intestine. We demonstrate that downstream basolateral recycling regulators, GTPase RAB-10/Rab10 and BAR domain protein AMPH-1/Amphiphysin, bind to TBC-2 and help to recruit it to endosomes. In the absence of RAB-10 or AMPH-1 binding to TBC-2, RAB-5 membrane association is abnormally high and recycling cargo is trapped in early endosomes. Furthermore, the loss of TBC-2 or AMPH-1 leads to abnormally high spatial overlap of RAB-5 and RAB-10. Taken together our results indicate that RAB-10 and AMPH-1 mediated down-regulation of RAB-5 is an important step in recycling, required for cargo exit from early endosomes and regulation of early endosome–recycling endosome interactions.</p></div>", "links"=>[], "tags"=>["endosome", "elegans intestine", "Membrane Association", "recycling function", "rab", "master regulator", "gap", "cargo exit", "basolateral C", "recycling cargo", "basolateral recycling regulators", "amph", "Mediated Recruitment", "tbc"], "article_id"=>1552728, "categories"=>["Uncategorised"], "users"=>["Ou Liu", "Barth D. Grant"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1005514.s001", "https://dx.doi.org/10.1371/journal.pgen.1005514.s002", "https://dx.doi.org/10.1371/journal.pgen.1005514.s003", "https://dx.doi.org/10.1371/journal.pgen.1005514.s004", "https://dx.doi.org/10.1371/journal.pgen.1005514.s005", "https://dx.doi.org/10.1371/journal.pgen.1005514.s006"], "stats"=>{"downloads"=>7, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Basolateral_Endocytic_Recycling_Requires_RAB_10_and_AMPH_1_Mediated_Recruitment_of_RAB_5_GAP_TBC_2_to_Endosomes_/1552728", "title"=>"Basolateral Endocytic Recycling Requires RAB-10 and AMPH-1 Mediated Recruitment of RAB-5 GAP TBC-2 to Endosomes", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-09-22 03:41:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/2284026"], "description"=>"<p>All images are from deconvolved 3D confocal image stacks acquired in intact living animals expressing a GFP-tagged recycling cargo protein, the IL-2 receptor alpha-chain (hTAC-GFP) and tagRFP-RAB-5 in wild-type animals (A-A‴), <i>tbc-2(tm2241)</i> (B-B‴) and <i>amph-1(tm1060)</i> mutants (C-C‴). (A-A‴) In wild-type animals, hTAC-GFP labels both punctate and tubular endosomal structures and has very little overlap with tagRFP-RAB-5. (B-B‴) In <i>tbc-2</i> mutants, hTAC-GFP and tagRFP-RAB-5 label abnormally enlarged endosomal structures. There is a striking increase in the degree of overlap between hTAC-GFP and tagRFP-RAB-5 in <i>tbc-2</i> mutants in comparison to that in wild-type animals. (C-C‴) In <i>amph-1</i> mutants, the degree of colocalization between hTAC-GFP and tagRFP-RAB-5 also increased. In each image autofluorescent lysosome-like organelles appear in blue in all three channels, whereas GFP appears only in the green channel and RFP appears only in the red channel. Signals observed in the green or red channels that do not overlap with signals in the blue channel are considered bona fide GFP or RFP signals respectively. (Scale bar: 10 μm) (D) Pearson's correlation coefficient for colocalization of hTAC-GFP and tagRFP-RAB-5. n = 6. Error bars represent SEM. ***P<0.001(student's t test).</p>", "links"=>[], "tags"=>["endosome", "elegans intestine", "Membrane Association", "recycling function", "rab", "master regulator", "gap", "cargo exit", "basolateral C", "recycling cargo", "basolateral recycling regulators", "amph", "Mediated Recruitment", "tbc"], "article_id"=>1552726, "categories"=>["Uncategorised"], "users"=>["Ou Liu", "Barth D. Grant"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005514.g007", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Recycling_cargo_accumulates_in_RAB_5_labeled_endosomes_in_tbc_2_and_amph_1_mutants_/1552726", "title"=>"Recycling cargo accumulates in RAB-5 labeled endosomes in <i>tbc-2</i> and <i>amph-1</i> mutants.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-22 03:41:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/2284009"], "description"=>"<p>(A) The membrane-to-cytosol ratio of RAB-5 increased in <i>tbc-2</i>, <i>rab-10</i> and <i>amph-1</i> mutants. Post-nuclear worm lysates were subject to centrifugation at 100,000g for 1 hour. 100P corresponds to the pellets and 100S represents the supernatants after the 100,000g centrifugation. (B) Loading control with actin antibody. (C) Quantification of the membrane-to-cytosol ratio (100P/100S) of RAB-5 in appropriate genetic backgrounds. The ratio of membrane-bound VS cytosolic RAB-5 was determined by densitometry. The standard deviations from three independent experiments are shown. **P<0.01 (student's t test).</p>", "links"=>[], "tags"=>["endosome", "elegans intestine", "Membrane Association", "recycling function", "rab", "master regulator", "gap", "cargo exit", "basolateral C", "recycling cargo", "basolateral recycling regulators", "amph", "Mediated Recruitment", "tbc"], "article_id"=>1552723, "categories"=>["Uncategorised"], "users"=>["Ou Liu", "Barth D. Grant"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005514.g005", "stats"=>{"downloads"=>0, "page_views"=>22, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_RAB_5_displays_elevated_membrane_association_in_tbc_2_rab_10_and_amph_1_mutants_/1552723", "title"=>"RAB-5 displays elevated membrane-association in <i>tbc-2</i>, <i>rab-10</i> and <i>amph-1</i> mutants.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-22 03:41:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/2284008"], "description"=>"<p>All images are from deconvolved 3D confocal image stacks acquired in intact living animals expressing GFP- and RFP-tagged proteins. (A-E) Representative confocal images of the worm intestine expressing a GFP-tagged recycling cargo protein, the human transferrin receptor (hTFR-GFP). Loss of TBC-2 caused accumulation of hTFR-GFP on abnormally enlarged endosomal structures. The <i>tbc-2</i> mutant phenotype in cargo recycling is rescued by expression of RFP-tagged full-length TBC-2 in the worm intestine. However, expression of mutant forms of RFP-tagged TBC-2 defective either in AMPH-1-binding (TBC-2[P150A]) or in RAB-10-binding (TBC-2[288–292 AAAAA]) in the worm intestine failed to rescue the <i>tbc-2</i> mutant phenotype. (C') Confocal image of the worm intestine expressing RFP-tagged wild-type TBC-2. (D') Confocal image of the worm intestine expressing RFP-tagged mutant form of TBC-2 with alanine substitution at proline P150. (E') Confocal image of the worm intestine expressing RFP-tagged mutant form of TBC-2 with five alanines replacing amino acids 288–292. (Scale bar: 10 μm) (F) Quantification of the average total intensity of hTFR-GFP. In each image autofluorescent lysosome-like organelles appears in blue in all three channels, whereas GFP appears only in the green channel and RFP shows up only in the red channel. Signals observed in the green or red channels that do not overlap with signals in the blue channel are considered bona fide GFP or RFP signals respectively. (n = 18 each, 6 animals of each genotype sampled in three different regions of each intestine.) Error bars represent SEM. **P<0.01, ***P<0.001(student's t test).</p>", "links"=>[], "tags"=>["endosome", "elegans intestine", "Membrane Association", "recycling function", "rab", "master regulator", "gap", "cargo exit", "basolateral C", "recycling cargo", "basolateral recycling regulators", "amph", "Mediated Recruitment", "tbc"], "article_id"=>1552722, "categories"=>["Uncategorised"], "users"=>["Ou Liu", "Barth D. Grant"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1005514.g004", "stats"=>{"downloads"=>3, "page_views"=>21, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Rescue_of_the_cargo_recycling_defect_of_tbc_2_mutants_requires_intact_RAB_10_and_AMPH_1_interaction_sequences_/1552722", "title"=>"Rescue of the cargo-recycling defect of <i>tbc-2</i> mutants requires intact RAB-10 and AMPH-1 interaction sequences.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-22 03:41:46"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"43", "full-text"=>"39", "pdf"=>"27", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"35", "full-text"=>"25", "pdf"=>"20", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"43", "full-text"=>"19", "pdf"=>"27", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"20", "full-text"=>"21", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"19", "full-text"=>"21", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"23", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"20", "full-text"=>"21", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"16", "full-text"=>"9", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"19", "full-text"=>"16", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"26", "full-text"=>"25", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"15", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"22", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"9", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"20", "full-text"=>"25", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"14", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"6", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"19", "full-text"=>"20", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts