Trehalose Accumulation Triggers Autophagy during Plant Desiccation
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{"title"=>"Trehalose Accumulation Triggers Autophagy during Plant Desiccation", "type"=>"journal", "authors"=>[{"first_name"=>"Brett", "last_name"=>"Williams", "scopus_author_id"=>"36090521300"}, {"first_name"=>"Isaac", "last_name"=>"Njaci", "scopus_author_id"=>"45961537100"}, {"first_name"=>"Lalehvash", "last_name"=>"Moghaddam", "scopus_author_id"=>"11940871700"}, {"first_name"=>"Hao", "last_name"=>"Long", "scopus_author_id"=>"57197444034"}, {"first_name"=>"Martin B.", "last_name"=>"Dickman", "scopus_author_id"=>"7005833013"}, {"first_name"=>"Xiuren", "last_name"=>"Zhang", "scopus_author_id"=>"8609705600"}, {"first_name"=>"Sagadevan", "last_name"=>"Mundree", "scopus_author_id"=>"6603259979"}], "year"=>2015, "source"=>"PLoS Genetics", "identifiers"=>{"issn"=>"15537404", "scopus"=>"2-s2.0-84953226488", "pui"=>"607579400", "doi"=>"10.1371/journal.pgen.1005705", "isbn"=>"1553-7404", "sgr"=>"84953226488", "pmid"=>"26633550"}, "id"=>"98a27dee-ad3e-3f01-a736-ba45dbb7ce99", "abstract"=>"Global climate change, increasingly erratic weather and a burgeoning global population are significant threats to the sustainability of future crop production. There is an urgent need for the dWilliams, B., Njaci, I., Moghaddam, L., Long, H., Dickman, M. B., Zhang, X., & Mundree, S. (2015). Trehalose Accumulation Triggers Autophagy during Plant Desiccation. PLoS Genetics, 11(12), 1–17. doi:10.1371/journal.pgen.1005705evelopment of robust measures that enable crops to withstand the uncertainty of climate change whilst still producing maximum yields. Resurrection plants possess the unique ability to withstand desiccation for prolonged periods, can be restored upon watering and represent great potential for the development of stress tolerant crops. Here, we describe the remarkable stress characteristics of Tripogon loliiformis, an uncharacterised resurrection grass and close relative of the economically important cereals, rice, sorghum, and maize. We show that T. loliiformis survives extreme environmental stress by implementing autophagy to prevent Programmed Cell Death. Notably, we identified a novel role for trehalose in the regulation of autophagy in T.loliiformis. Transcriptome, Gas Chromatography Mass Spectrometry, immunoblotting and confocal microscopy analyses directly linked the accumulation of trehalose with the onset of autophagy in dehydrating and desiccated T. loliiformis shoots. These results were supported in vitro with the observation of autophagosomes in trehalose treated T. loliiformis leaves; autophagosomes were not detected in untreated samples. Presumably, once induced, autophagy promotes desiccation tolerance in T.loliiformis, by removal of cellular toxins to suppress programmed cell death and the recycling of nutrients to delay the onset of senescence. These findings illustrate how resurrection plants manipulate sugar metabolism to promote desiccation tolerance and may provide candidate genes that are potentially useful for the development of stress tolerant crops.", "link"=>"http://www.mendeley.com/research/trehalose-accumulation-triggers-autophagy-during-plant-desiccation", "reader_count"=>62, "reader_count_by_academic_status"=>{"Unspecified"=>4, "Professor > Associate Professor"=>2, "Researcher"=>11, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>17, "Student > Postgraduate"=>6, "Student > Master"=>11, "Other"=>1, "Student > Bachelor"=>5, "Professor"=>4}, "reader_count_by_user_role"=>{"Unspecified"=>4, "Professor > Associate Professor"=>2, "Researcher"=>11, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>17, "Student > Postgraduate"=>6, "Student > Master"=>11, "Other"=>1, "Student > Bachelor"=>5, "Professor"=>4}, "reader_count_by_subject_area"=>{"Unspecified"=>7, "Biochemistry, Genetics and Molecular Biology"=>10, "Materials Science"=>1, "Agricultural and Biological Sciences"=>44}, "reader_count_by_subdiscipline"=>{"Materials Science"=>{"Materials Science"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>44}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>10}, "Unspecified"=>{"Unspecified"=>7}}, "reader_count_by_country"=>{"Czech Republic"=>1, "United States"=>1, "South Africa"=>1, "Chile"=>1, "Germany"=>1}, "group_count"=>1}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/4436116"], "description"=>"<p><i>Tripogon loliiformis</i> uses a multi-pronged strategy to survive desiccation stress. I) rapid shutdown of photosynthesis triggers caloric deficiency and the onset of autophagy pathways during the early stages of dehydration. ii) autophagic removal of damaged, unfolded and redundant organelles and proteins as well as ROS mitigates stress to delay the onset of apoptosis. The efficacy of autophagy pathways is bolstered by the accumulation of trehalose throughout dehydration. iii) Nutrient recycling as a product of autophagy mediated breakdown of proteins and organelles suspends “aging” senescence pathways.</p>", "links"=>[], "tags"=>["uncharacterised resurrection grass", "onset", "autophagosome", "future crop production", "Gas Chromatography Mass Spectrometry", "autophagy", "T.loliiformi", "climate change whilst", "desiccation tolerance", "confocal microscopy analyses", "trehalose", "Plant Desiccation Global climate change", "Programmed Cell Death", "loliiformi", "stress", "Trehalose Accumulation Triggers Autophagy"], "article_id"=>1618626, "categories"=>["Cell Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Ecology", "Biological Sciences not elsewhere classified", "Plant Biology"], "users"=>["Brett Williams", "Isaac Njaci", "Lalehvash Moghaddam", "Hao Long", "Martin B Dickman", "Xiuren Zhang", "Sagadevan Mundree"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1005705.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Autophagy_mediated_desiccation_tolerance_in_Tripogon_loliiformis_/1618626", "title"=>"Autophagy-mediated desiccation tolerance in <i>Tripogon loliiformis</i>.", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-12-03 14:12:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/4436092"], "description"=>"<p><i>T</i>. <i>loliiformis</i> plants were grown from seed harvested from a single source and grown in the glasshouse for two months, dehydrated to desiccation and rehydrated with the addition of water. Left Panels—fully hydrated (Top), dehydrating and desiccated (Middle) and 48h after rehydration (Bottom). Right Panels—Leaf tissue was harvested from hydrated and dehydrating plants at 60, 40 and <10% RWC and stained with Evans Blue. As a control, a hydrated leaf was stained after immersion in boiling water for 5 minutes (samples as labelled).</p>", "links"=>[], "tags"=>["uncharacterised resurrection grass", "onset", "autophagosome", "future crop production", "Gas Chromatography Mass Spectrometry", "autophagy", "T.loliiformi", "climate change whilst", "desiccation tolerance", "confocal microscopy analyses", "trehalose", "Plant Desiccation Global climate change", "Programmed Cell Death", "loliiformi", "stress", "Trehalose Accumulation Triggers Autophagy"], "article_id"=>1618611, "categories"=>["Cell Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Ecology", "Biological Sciences not elsewhere classified", "Plant Biology"], "users"=>["Brett Williams", "Isaac Njaci", "Lalehvash Moghaddam", "Hao Long", "Martin B Dickman", "Xiuren Zhang", "Sagadevan Mundree"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1005705.g001"], "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Tripogon_loliiformis_leaf_tissues_are_alive_at_desiccation_/1618611", "title"=>"<i>Tripogon loliiformis</i> leaf tissues are alive at desiccation.", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-12-03 14:12:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/4436101"], "description"=>"<p>Hydrated, dehydrating, desiccated and rehydrated <i>T</i>. <i>loliiformis</i> leaf tissues were harvested and subjected to TUNEL assay and propidium iodide counter staining for the detection of DNA fragmentation and Apoptotic-like cell death. Hydrated leaves treated with DNase were used as the positive control.</p>", "links"=>[], "tags"=>["uncharacterised resurrection grass", "onset", "autophagosome", "future crop production", "Gas Chromatography Mass Spectrometry", "autophagy", "T.loliiformi", "climate change whilst", "desiccation tolerance", "confocal microscopy analyses", "trehalose", "Plant Desiccation Global climate change", "Programmed Cell Death", "loliiformi", "stress", "Trehalose Accumulation Triggers Autophagy"], "article_id"=>1618619, "categories"=>["Cell Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Ecology", "Biological Sciences not elsewhere classified", "Plant Biology"], "users"=>["Brett Williams", "Isaac Njaci", "Lalehvash Moghaddam", "Hao Long", "Martin B Dickman", "Xiuren Zhang", "Sagadevan Mundree"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1005705.g002"], "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_T_loliiformis_suppresses_PCD_pathway_during_dehydration_and_desiccation_/1618619", "title"=>"<i>T</i>. <i>loliiformis</i> suppresses PCD pathway during dehydration and desiccation.", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-12-03 14:12:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/4436107"], "description"=>"<p>Dehydrating (60, 40%RWC) and completely desiccated (<10% RWC) <i>T</i>. <i>loliiformis</i> leaves were harvested and subjected to Immunoblotting using an ATG8 antibody. ATG8 proteins were made visible by immunoblot analysis with a polyclonal ATG8 antibody following separation by Urea-SDS PAGE*. Approximately 30 μg of total protein as calculated by Bradford assay was loaded for each sample (Ponceau staining of PVDF membrane post-transfer). Protein extracts were subjected to phospholipase D treatment to verify ATG8 lipidation. Signal intensities of the ATG8-PE band were calculated using ImageJ software and were normalised using the Ponceau stained membrane post-transfer for normalisation. Each bar represents the mean ± SEM of triplicate values from a representative experiment. RWC = relative water content, U = undigested, D = digested.</p>", "links"=>[], "tags"=>["uncharacterised resurrection grass", "onset", "autophagosome", "future crop production", "Gas Chromatography Mass Spectrometry", "autophagy", "T.loliiformi", "climate change whilst", "desiccation tolerance", "confocal microscopy analyses", "trehalose", "Plant Desiccation Global climate change", "Programmed Cell Death", "loliiformi", "stress", "Trehalose Accumulation Triggers Autophagy"], "article_id"=>1618620, "categories"=>["Cell Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Ecology", "Biological Sciences not elsewhere classified", "Plant Biology"], "users"=>["Brett Williams", "Isaac Njaci", "Lalehvash Moghaddam", "Hao Long", "Martin B Dickman", "Xiuren Zhang", "Sagadevan Mundree"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1005705.g003"], "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Tripogon_loliiformis_employs_autophagy_as_a_pro_survival_mechanism_during_desiccation_/1618620", "title"=>"<i>Tripogon loliiformis</i> employs autophagy as a pro-survival mechanism during desiccation.", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-12-03 14:12:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/4436050", "https://ndownloader.figshare.com/files/4436053", "https://ndownloader.figshare.com/files/4436062", "https://ndownloader.figshare.com/files/4436065", "https://ndownloader.figshare.com/files/4436068", "https://ndownloader.figshare.com/files/4436077", "https://ndownloader.figshare.com/files/4436080"], "description"=>"<div><p>Global climate change, increasingly erratic weather and a burgeoning global population are significant threats to the sustainability of future crop production. There is an urgent need for the development of robust measures that enable crops to withstand the uncertainty of climate change whilst still producing maximum yields. Resurrection plants possess the unique ability to withstand desiccation for prolonged periods, can be restored upon watering and represent great potential for the development of stress tolerant crops. Here, we describe the remarkable stress characteristics of <i>Tripogon loliiformis</i>, an uncharacterised resurrection grass and close relative of the economically important cereals, rice, sorghum, and maize. We show that <i>T</i>. <i>loliiformis</i> survives extreme environmental stress by implementing autophagy to prevent Programmed Cell Death. Notably, we identified a novel role for trehalose in the regulation of autophagy in <i>T</i>.<i>loliiformis</i>. Transcriptome, Gas Chromatography Mass Spectrometry, immunoblotting and confocal microscopy analyses directly linked the accumulation of trehalose with the onset of autophagy in dehydrating and desiccated <i>T</i>. <i>loliiformis</i> shoots. These results were supported <i>in vitro</i> with the observation of autophagosomes in trehalose treated <i>T</i>. <i>loliiformis</i> leaves; autophagosomes were not detected in untreated samples. Presumably, once induced, autophagy promotes desiccation tolerance in <i>T</i>.<i>loliiformis</i>, by removal of cellular toxins to suppress programmed cell death and the recycling of nutrients to delay the onset of senescence. These findings illustrate how resurrection plants manipulate sugar metabolism to promote desiccation tolerance and may provide candidate genes that are potentially useful for the development of stress tolerant crops.</p></div>", "links"=>[], "tags"=>["uncharacterised resurrection grass", "onset", "autophagosome", "future crop production", "Gas Chromatography Mass Spectrometry", "autophagy", "T.loliiformi", "climate change whilst", "desiccation tolerance", "confocal microscopy analyses", "trehalose", "Plant Desiccation Global climate change", "Programmed Cell Death", "loliiformi", "stress", "Trehalose Accumulation Triggers Autophagy"], "article_id"=>1618642, "categories"=>["Cell Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Ecology", "Biological Sciences not elsewhere classified", "Plant Biology"], "users"=>["Brett Williams", "Isaac Njaci", "Lalehvash Moghaddam", "Hao Long", "Martin B Dickman", "Xiuren Zhang", "Sagadevan Mundree"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1005705.s001", "https://dx.doi.org/10.1371/journal.pgen.1005705.s002", "https://dx.doi.org/10.1371/journal.pgen.1005705.s003", "https://dx.doi.org/10.1371/journal.pgen.1005705.s004", "https://dx.doi.org/10.1371/journal.pgen.1005705.s005", "https://dx.doi.org/10.1371/journal.pgen.1005705.s006", "https://dx.doi.org/10.1371/journal.pgen.1005705.s007"], "stats"=>{"downloads"=>9, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Trehalose_Accumulation_Triggers_Autophagy_during_Plant_Desiccation_/1618642", "title"=>"Trehalose Accumulation Triggers Autophagy during Plant Desiccation", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-12-03 14:12:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/4436113"], "description"=>"<p>Hydrated leaves from three month old glasshouse grown <i>T</i>. <i>loliiformis</i> plants were harvested, divided into 1 cm segments, vacuum infiltrated with 1 and 5 mM trehalose solution containing 1 μM concamycin A prepared in ½ MS basal salts and incubated for 24 hrs. Autophagosomes were visualised by Transmission Electron Microscopy. Top Panel–(A) Concanamycin control, (B) 1 mM trehalose, (C) 1 mM trehalose, higher magnification, (D) 5 mM trehalose, (E) 5 mM trehalose, higher magnification, (F) Tunicamycin control, Bottom panel—The sucrose and trehalose contents of hydrated, dehydrating and desiccated leaves were assessed by GCMS analysis.</p>", "links"=>[], "tags"=>["uncharacterised resurrection grass", "onset", "autophagosome", "future crop production", "Gas Chromatography Mass Spectrometry", "autophagy", "T.loliiformi", "climate change whilst", "desiccation tolerance", "confocal microscopy analyses", "trehalose", "Plant Desiccation Global climate change", "Programmed Cell Death", "loliiformi", "stress", "Trehalose Accumulation Triggers Autophagy"], "article_id"=>1618623, "categories"=>["Cell Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Ecology", "Biological Sciences not elsewhere classified", "Plant Biology"], "users"=>["Brett Williams", "Isaac Njaci", "Lalehvash Moghaddam", "Hao Long", "Martin B Dickman", "Xiuren Zhang", "Sagadevan Mundree"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1005705.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Exogenous_application_of_trehalose_triggers_autophagy_in_T_loliiformis_/1618623", "title"=>"Exogenous application of trehalose triggers autophagy in <i>T</i>.<i>loliiformis</i>.", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-12-03 14:12:18"}

PMC Usage Stats | Further Information

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Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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