The ESCRT regulator Did2 maintains the balance between long-distance endosomal transport and endocytic trafficking
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"The ESCRT regulator Did2 maintains the balance between long-distance endosomal transport and endocytic trafficking", "type"=>"journal", "authors"=>[{"first_name"=>"Carl", "last_name"=>"Haag", "scopus_author_id"=>"35423800600"}, {"first_name"=>"Thomas", "last_name"=>"Pohlmann", "scopus_author_id"=>"36879735600"}, {"first_name"=>"Michael", "last_name"=>"Feldbrügge", "scopus_author_id"=>"55921718100"}], "year"=>2017, "source"=>"PLoS Genetics", "identifiers"=>{"scopus"=>"2-s2.0-85018428120", "doi"=>"10.1371/journal.pgen.1006734", "sgr"=>"85018428120", "isbn"=>"1111111111", "pmid"=>"28422978", "issn"=>"15537404", "pui"=>"615859574"}, "id"=>"a86140f0-2459-386b-a558-1da187165b4f", "abstract"=>"In highly polarised cells, like fungal hyphae, early endosomes function in both endocytosis as well as long-distance transport of various cargo including mRNA and protein complexes. However, knowledge on the crosstalk between these seemingly different trafficking pro-cesses is scarce. Here, we demonstrate that the ESCRT regulator Did2 coordinates endo-somal transport in fungal hyphae of Ustilago maydis. Loss of Did2 results in defective vacuolar targeting, less processive long-distance transport and abnormal shuttling of early endosomes. Importantly, the late endosomal protein Rab7 and vacuolar protease Prc1 exhibit increased shuttling on these aberrant endosomes suggesting defects in endosomal maturation and identity. Consistently, molecular motors fail to attach efficiently explaining the disturbed processive movement. Furthermore, the endosomal mRNP linker protein Upa1 is hardly present on endosomes resulting in defects in long-distance mRNA transport. In conclusion, the ESCRT regulator Did2 coordinates precise maturation of endosomes and thus provides the correct membrane identity for efficient endosomal long-distance transport. Author summary Eukaryotic cells rely on constant membrane trafficking to organise their intracellular compartments. Endosomes are important transport units that serve as sorting platforms during endocytosis. Internalised cargo destined for degradation is transferred via matur-ing endosomes towards the lysosome/vacuole. Essential for this maturation and mem-brane remodelling is the so-called ESCRT machinery. In highly polarised cells, like fungal hyphae, endosomes carry out an additional role: they transport various cargos such as mRNAs and protein complexes over long distances. At present it is unclear how these two seemingly independent functions are coordinated. Here we demonstrate in the plant path-ogen Ustilago maydis that the ESCRT regulator Did2 is required for the dual function of endosomes. Loss of Did2 resulted in defective targeting of vacuolar proteins, disturbed endosomal mRNA-transport as well as altered shuttling of endosomes. Importantly, we observed that in absence of Did2 proteins typically present on static late endosomes or in vacuoles are now present on motile early endosomes. Based on these findings, we propose", "link"=>"http://www.mendeley.com/research/escrt-regulator-did2-maintains-balance-between-longdistance-endosomal-transport-endocytic-traffickin", "reader_count"=>9, "reader_count_by_academic_status"=>{"Student > Ph. D. Student"=>4, "Other"=>1, "Student > Bachelor"=>2, "Professor"=>1, "Unspecified"=>1}, "reader_count_by_user_role"=>{"Student > Ph. D. Student"=>4, "Other"=>1, "Student > Bachelor"=>2, "Professor"=>1, "Unspecified"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>4, "Neuroscience"=>2, "Unspecified"=>1}, "reader_count_by_subdiscipline"=>{"Neuroscience"=>{"Neuroscience"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/85018428120"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/85018428120?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85018428120&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85018428120&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/85018428120", "dc:identifier"=>"SCOPUS_ID:85018428120", "eid"=>"2-s2.0-85018428120", "dc:title"=>"The ESCRT regulator Did2 maintains the balance between long-distance endosomal transport and endocytic trafficking", "dc:creator"=>"Haag C.", "prism:publicationName"=>"PLoS Genetics", "prism:issn"=>"15537390", "prism:eIssn"=>"15537404", "prism:volume"=>"13", "prism:issueIdentifier"=>"4", "prism:pageRange"=>nil, "prism:coverDate"=>"2017-04-01", "prism:coverDisplayDate"=>"April 2017", "prism:doi"=>"10.1371/journal.pgen.1006734", "citedby-count"=>"10", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Heinrich-Heine-Universität Düsseldorf", "affiliation-city"=>"Dusseldorf", "affiliation-country"=>"Germany"}], "pubmed-id"=>"28422978", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e1006734", "source-id"=>"4000151808", "openaccess"=>"1", "openaccessFlag"=>true}

Counter

  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"98", "xml_views"=>"14", "html_views"=>"838"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"76", "xml_views"=>"7", "html_views"=>"510"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"79"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"41", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"41"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"3", "html_views"=>"10"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"22", "xml_views"=>"2", "html_views"=>"18"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"34", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"10", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"9"}

PMC Usage Stats

  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"28", "full-text"=>"21", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"24", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"25", "full-text"=>"20", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"33", "full-text"=>"17", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"15", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"15", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"20", "full-text"=>"13", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"8", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"17", "full-text"=>"11", "pdf"=>"24", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"14", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"11", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"11", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"4", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"15", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"11", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"23", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"10", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"17", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"9"}
Loading … Spinner
There are currently no alerts