Use of a microfluidic platform to uncover basic features of energy and environmental stress responses in individual cells of Bacillus subtilis
Publication Date
July 20, 2017
Authors
Matthew T. Cabeen, Jonathan R. Russell, Johan Paulsson & Richard Losick
Volume
13
Issue
7
Pages
e1006901
DOI
https://dx.plos.org/10.1371/journal.pgen.1006901
Publisher URL
http://journals.plos.org/plosgenetics/article?id=10.1371%2Fjournal.pgen.1006901
Scopus
85026650520
Mendeley
http://www.mendeley.com/research/microfluidic-platform-uncover-basic-features-energy-environmental-stress-responses-individual-cells
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Use of a microfluidic platform to uncover basic features of energy and environmental stress responses in individual cells of Bacillus subtilis", "type"=>"journal", "authors"=>[{"first_name"=>"Matthew T.", "last_name"=>"Cabeen", "scopus_author_id"=>"8704680500"}, {"first_name"=>"Jonathan R.", "last_name"=>"Russell", "scopus_author_id"=>"57195259270"}, {"first_name"=>"Johan", "last_name"=>"Paulsson", "scopus_author_id"=>"6603910605"}, {"first_name"=>"Richard", "last_name"=>"Losick", "scopus_author_id"=>"7101975024"}], "year"=>2017, "source"=>"PLoS Genetics", "identifiers"=>{"scopus"=>"2-s2.0-85026650520", "sgr"=>"85026650520", "issn"=>"15537404", "doi"=>"10.1371/journal.pgen.1006901", "pmid"=>"28727759", "isbn"=>"1111111111", "pui"=>"617638970"}, "id"=>"5ad4a765-380e-3d69-a141-ad3a8ceb2b21", "abstract"=>"Bacteria use a variety of stress-sensing systems to sense and respond to diverse stressors and to ensure their survival under adverse conditions. The gram-positive bacterium Bacillus subtilis responds to energy stress (ATP depletion) and to environmental stressors using two distinct stress-sensing pathways that converge on the alternative sigma factor σB to provoke a general stress response. Past efforts to study the σB stress response in bulk culture and on agarose pads were unable to visualize the responses of individual cells under tightly controlled conditions for extended periods of time. Here we use a microfluidics-based strategy to discern the basic features of σB activation in single cells in response to energy and environmental stress, both immediately upon stressor exposure and for tens of generations thereafter. Upon energy stress at various levels of stressor, cells exhibited fast, transient, and amplitude-modulated responses but not frequency modulation as previously reported. Upon environmental stress, which is mediated by the stressosome complex, wild-type cells primarily exhibited a transient and amplitude-modulated response. However, mutant cells producing only one of the four paralogous RsbR stressosome proteins showed striking and previously unseen differences. Whereas RsbRA-only cells mimicked the wild type, RsbRC-only cells displayed a slower but sustained overall response composed of repeated activation events in single cells.", "link"=>"http://www.mendeley.com/research/microfluidic-platform-uncover-basic-features-energy-environmental-stress-responses-individual-cells", "reader_count"=>15, "reader_count_by_academic_status"=>{"Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>1, "Student > Master"=>3, "Student > Bachelor"=>2, "Professor"=>1, "Unspecified"=>1}, "reader_count_by_user_role"=>{"Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>1, "Student > Master"=>3, "Student > Bachelor"=>2, "Professor"=>1, "Unspecified"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>7, "Agricultural and Biological Sciences"=>4, "Physics and Astronomy"=>2, "Chemical Engineering"=>1, "Unspecified"=>1}, "reader_count_by_subdiscipline"=>{"Physics and Astronomy"=>{"Physics and Astronomy"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>7}, "Unspecified"=>{"Unspecified"=>1}, "Chemical Engineering"=>{"Chemical Engineering"=>1}}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/85026650520"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/85026650520?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85026650520&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85026650520&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/85026650520", "dc:identifier"=>"SCOPUS_ID:85026650520", "eid"=>"2-s2.0-85026650520", "dc:title"=>"Use of a microfluidic platform to uncover basic features of energy and environmental stress responses in individual cells of Bacillus subtilis", "dc:creator"=>"Cabeen M.", "prism:publicationName"=>"PLoS Genetics", "prism:issn"=>"15537390", "prism:eIssn"=>"15537404", "prism:volume"=>"13", "prism:issueIdentifier"=>"7", "prism:pageRange"=>nil, "prism:coverDate"=>"2017-07-01", "prism:coverDisplayDate"=>"July 2017", "prism:doi"=>"10.1371/journal.pgen.1006901", "citedby-count"=>"15", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Harvard University", "affiliation-city"=>"Cambridge", "affiliation-country"=>"United States"}], "pubmed-id"=>"28727759", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e1006901", "source-id"=>"4000151808", "openaccess"=>"1", "openaccessFlag"=>true}

Counter

  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"65", "xml_views"=>"6", "html_views"=>"734"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"76", "xml_views"=>"0", "html_views"=>"795"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"65", "xml_views"=>"3", "html_views"=>"134"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"25", "xml_views"=>"1", "html_views"=>"115"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"60", "xml_views"=>"0", "html_views"=>"186"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"109"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"55", "xml_views"=>"0", "html_views"=>"66"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"36", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"36", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"43", "xml_views"=>"1", "html_views"=>"60"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"32", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"26", "xml_views"=>"2", "html_views"=>"28"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"2", "html_views"=>"34"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"41"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"24", "xml_views"=>"4", "html_views"=>"50"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"12", "xml_views"=>"2", "html_views"=>"40"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"9", "xml_views"=>"2", "html_views"=>"25"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"38", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"28", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"34", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"16"}

PMC Usage Stats

  • {"unique-ip"=>"17", "full-text"=>"12", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"35", "full-text"=>"23", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"24", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"34", "full-text"=>"16", "pdf"=>"18", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"50", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"21", "full-text"=>"21", "pdf"=>"22", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"15", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"18", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"16", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"11", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"7", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"17", "full-text"=>"12", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"11", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"5", "cited-by"=>"2", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"11", "cited-by"=>"1", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"19", "full-text"=>"14", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"12", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"12", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"3", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"25", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"29", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
Loading … Spinner
There are currently no alerts