Trichinella spiralis Paramyosin Binds to C8 and C9 and Protects the Tissue-Dwelling Nematode from Being Attacked by Host Complement
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{"title"=>"Trichinella spiralis paramyosin binds to c8 and c9 and protects the tissue-dwelling nematode from being attacked by host complement", "type"=>"journal", "authors"=>[{"first_name"=>"Zhifei", "last_name"=>"Zhang", "scopus_author_id"=>"57192634766"}, {"first_name"=>"Jing", "last_name"=>"Yang", "scopus_author_id"=>"57037703700"}, {"first_name"=>"Junfei", "last_name"=>"Wei", "scopus_author_id"=>"56221989800"}, {"first_name"=>"Yaping", "last_name"=>"Yang", "scopus_author_id"=>"56157000700"}, {"first_name"=>"Xiaoqin", "last_name"=>"Chen", "scopus_author_id"=>"36097726400"}, {"first_name"=>"Xi", "last_name"=>"Zhao", "scopus_author_id"=>"55598909300"}, {"first_name"=>"Yuan", "last_name"=>"Gu", "scopus_author_id"=>"35752021500"}, {"first_name"=>"Shijuan", "last_name"=>"Cui", "scopus_author_id"=>"26027825100"}, {"first_name"=>"Xinping", "last_name"=>"Zhu", "scopus_author_id"=>"8600256500"}], "year"=>2011, "source"=>"PLoS Neglected Tropical Diseases", "identifiers"=>{"sgr"=>"79960933692", "pui"=>"362246854", "doi"=>"10.1371/journal.pntd.0001225", "pmid"=>"21750743", "scopus"=>"2-s2.0-79960933692", "issn"=>"19352727"}, "id"=>"47874db4-b760-3051-9b96-8b376651630f", "abstract"=>"BACKGROUND: Paramyosin is a thick myofibrillar protein found exclusively in invertebrates. Evidence suggested that paramyosin from helminths serves not only as a structural protein but also as an immunomodulatory agent. We previously reported that recombinant Trichinella spiralis paramyosin (Ts-Pmy) elicited a partial protective immunity in mice. In this study, the ability of Ts-Pmy to bind host complement components and protect against host complement attack was investigated.\\n\\nMETHODS AND FINDINGS: In this study, the transcriptional and protein expression levels of Ts-Pmy were determined in T. spiralis newborn larva (NBL), muscle larva (ML) and adult worm developmental stages by RT-PCR and western blot analysis. Expression of Ts-Pmy at the outer membrane was observed in NBL and adult worms using immunogold electron microscopy and immunofluorescence staining. Functional analysis revealed that recombinant Ts-Pmy(rTs-Pmy) strongly bound to complement components C8 and C9 and inhibited the polymerization of C9 during the formation of the membrane attack complex (MAC). rTs-Pmy also inhibited the lysis of rabbit erythrocytes (E(R)) elicited by an alternative pathway-activated complement from guinea pig serum. Inhibition of native Ts-Pmy on the surface of NBL with a specific antiserum reduced larvae viability when under the attack of complement in vitro. In vivo passive transfer of anti-Ts-Pmy antiserum and complement-treated larvae into mice also significantly reduced the number of larvae that developed to ML.\\n\\nCONCLUSION: These studies suggest that the outer membrane form of T. spiralis paramyosin plays an important role in the evasion of the host complement attack.", "link"=>"http://www.mendeley.com/research/trichinella-spiralis-paramyosin-binds-c8-c9-protects-tissuedwelling-nematode-attacked-host-complemen", "reader_count"=>7, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>4, "Student > Master"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>4, "Student > Master"=>1}, "reader_count_by_subject_area"=>{"Agricultural and Biological Sciences"=>5, "Medicine and Dentistry"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>5}}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/759526"], "description"=>"<p>The NHS was incubated with r<i>Ts</i>-Pmy (Lane 3) or with buffer only (Lane 2) before adding fresh E<sub>R</sub>. Lane 1, standard protein marker. Membrane debris of lysed E<sub>R</sub> was analyzed by SDS-PAGE under reducing conditions and Western blotting with anti-C9 mAb.</p>", "links"=>[], "tags"=>["deposition"], "article_id"=>429898, "categories"=>["Infectious Diseases"], "users"=>["Zhifei Zhang", "Jing Yang", "Junfei Wei", "Yaping Yang", "Xiaoqin Chen", "Xi Zhao", "Yuan Gu", "Shijuan Cui", "Xinping Zhu"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0001225.g008", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Inhibition_of_poly_C9_deposition_on_the_surface_of_E_R_by_r_Ts_Pmy_/429898", "title"=>"Inhibition of poly<i>-</i>C9 deposition on the surface of E<sub>R</sub> by r<i>Ts</i>-Pmy.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 17:00:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/759300"], "description"=>"<p>Human C8 (1 µg, Lane 1) and C9 (1 µg, Lane 3) and non-relevant BSA control (1 µg, Lane 2) were subjected to SDS-PAGE under reducing conditions and (A) stained with Coomassie blue, and then (B) transferred onto a PVDF membrane, incubated with r<i>Ts</i>-Pmy (5 µg/mL) and detected with anti-r<i>Ts-</i>Pmy mAb (50 ng/mL). The same materials that were added, plus non-relevant control protein r<i>Ts</i>87 (300 ng; Lane 4) were subjected to SDS-PAGE and (C) stained with Coomassie blue, and (D) transferred to an NC membrane, incubated with r<i>Ts</i>87 (5 µg/mL) and detected with anti-<i>Ts</i>87 rabbit serum (1∶4000). To determine the binding competition between soluble and immobilized C9 to r<i>Ts</i>-Pmy, r<i>Ts</i>-Pmy (5 µg/mL) was pre-incubated with C9 (30 µg/mL) for 1 hour before incubation with the membrane containing with C9 (1 µg) and BSA (1 µg), and was detected with anti-rTs-Pmy mAb (50 ng/mL) (E). M:standard protein marker.</p>", "links"=>[], "tags"=>["binding", "c8"], "article_id"=>429675, "categories"=>["Infectious Diseases"], "users"=>["Zhifei Zhang", "Jing Yang", "Junfei Wei", "Yaping Yang", "Xiaoqin Chen", "Xi Zhao", "Yuan Gu", "Shijuan Cui", "Xinping Zhu"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0001225.g005", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Determination_of_r_Ts_Pmy_binding_to_human_C8_and_C9_/429675", "title"=>"Determination of r<i>Ts</i>-Pmy binding to human C8 and C9.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 16:59:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/759024"], "description"=>"<p>(A) RT-PCR showing mRNA transcription of <i>Ts</i>-Pmy and GAPDH (control) from the different developmental stages of <i>T. spiralis</i>. Lane 1, DNA Marker; Lane 2, ML; Lane 4, adult worm; Lane 6, NBL. Lanes 3, 5 and 7: non-reverse transcriptase negative controls. (B) Western blot detection of native <i>Ts</i>-Pmy and GAPDH (control) expressed in different developmental stages of <i>T. spiralis</i> by specific antibodies with the Odyssey two color infrared imaging system. Lane 1, ML extracts (8 µg); Lane 2, adult worm extracts (8 µg); Lane 3, NBL extracts (8 µg); Lane 4, purified r<i>Ts</i>-Pmy (500 ng). (C). Electron micrographs of <i>T. spiralis</i> immunolabeled with anti-r<i>Ts-</i>Pmy mAb. NBL (a) and adult worm (b) were incubated with mouse anti-r<i>Ts-</i>Pmy mAb or normal mouse serum (c and d) and then with gold-conjugated protein A. Scale bars represent 0.2 µm. (D) Immunofluorescence micrographs of intact <i>T. spiralis</i> immunolabeled with rabbit antiserum against <i>Ts</i>-Pmy. Intact whole adult worms (a) and NBL (b) were incubated with anti-r<i>Ts-</i>Pmy rabbit serum or normal rabbit serum (c and d) and then with fluorescein isothiocyanate-conjugated goat anti-rabbit IgG. Scale bars represent 20 µm.</p>", "links"=>[], "tags"=>["immunolocalization", "stages"], "article_id"=>429390, "categories"=>["Infectious Diseases"], "users"=>["Zhifei Zhang", "Jing Yang", "Junfei Wei", "Yaping Yang", "Xiaoqin Chen", "Xi Zhao", "Yuan Gu", "Shijuan Cui", "Xinping Zhu"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0001225.g001", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_and_immunolocalization_of_Ts_Pmy_in_the_different_developmental_stages_of_T_spiralis_/429390", "title"=>"Expression and immunolocalization of <i>Ts</i>-Pmy in the different developmental stages of <i>T. spiralis</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 16:57:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/759115"], "description"=>"<p>NBL were incubated with different amounts of anti-r<i>Ts-</i>Pmy rabbit serum, the same serum pre-absorbed with r<i>Ts</i>-Pmy or normal rabbit serum (NRS) in a total volume of 50 µL for 60 min before adding fresh normal guinea pig serum (NGS). In addition, 40 µL of anti-<i>Ts</i>87 rabbit serum was used as a control. Viability of NBL was observed under an inverted microscope. Values are expressed as the means ± SD for three independent experiments. *, statistically significant differences (p<0.05) between anti-r<i>Ts</i>-Pmy and anti-r<i>Ts</i>-Pmy + r<i>Ts</i>-Pmy or NGS control.</p>", "links"=>[], "tags"=>["complement-mediated", "nbl", "incubated"], "article_id"=>429487, "categories"=>["Infectious Diseases"], "users"=>["Zhifei Zhang", "Jing Yang", "Junfei Wei", "Yaping Yang", "Xiaoqin Chen", "Xi Zhao", "Yuan Gu", "Shijuan Cui", "Xinping Zhu"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0001225.g002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Enhanced_complement_mediated_killing_of_T_spiralis_NBL_incubated_with_anti_r_Ts_Pmy_rabbit_serum_/429487", "title"=>"Enhanced complement-mediated killing of <i>T. spiralis</i> NBL incubated with anti-r<i>Ts-</i>Pmy rabbit serum.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 16:58:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/759184"], "description"=>"<p>Two thousand NBL were incubated with anti-r<i>Ts</i>-Pmy rabbit serum, the same serum absorbed with r<i>Ts-</i>Pmy or NRS (100 µL), followed by NGS or INGS. Treated NBL were then injected into BALB/c mice. ML were recovered from the muscle tissues of mice 26 days after injection. Data represent the means ± SD from three independent experiments. **, indicate statistically significant differences (p<0.01) between the antiserum-treated/NGS group and protein absorbed serum/NGS or NRS/NGS group; * indicate statistically significant differences (p = 0.048) between the antiserum-treated/INGS group and NRS/INGS group (n = 8 in each group).</p>", "links"=>[], "tags"=>["ml", "developed", "nbl", "treated", "serum"], "article_id"=>429552, "categories"=>["Infectious Diseases"], "users"=>["Zhifei Zhang", "Jing Yang", "Junfei Wei", "Yaping Yang", "Xiaoqin Chen", "Xi Zhao", "Yuan Gu", "Shijuan Cui", "Xinping Zhu"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0001225.g003", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Recovered_ML_developed_from_NBL_treated_with_anti_r_Ts_Pmy_rabbit_serum_and_NGS_/429552", "title"=>"Recovered ML developed from NBL treated with anti-r<i>Ts</i>-Pmy rabbit serum and NGS.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 16:58:33"}
  • {"files"=>["https://ndownloader.figshare.com/files/759251"], "description"=>"<p>Various amounts of r<i>Ts</i>-Pmy (0, 10, 20 or 40 µg) were incubated with fresh NGS(6%), and then fresh E<sub>R</sub> were added (3×10<sup>8</sup> cells). The amount of hemoglobin released into the supernatant was measured at 412 nm, and the percent lysis was calculated. The results are shown as the means ± SD for three independent experiments.</p>", "links"=>[], "tags"=>["complement-mediated", "lysis"], "article_id"=>429619, "categories"=>["Infectious Diseases"], "users"=>["Zhifei Zhang", "Jing Yang", "Junfei Wei", "Yaping Yang", "Xiaoqin Chen", "Xi Zhao", "Yuan Gu", "Shijuan Cui", "Xinping Zhu"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0001225.g004", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Inhibition_of_complement_mediated_lysis_of_E_R_by_r_Ts_Pmy_/429619", "title"=>"Inhibition of complement-mediated lysis of E<sub>R</sub> by r<i>Ts</i>-Pmy.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 16:58:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/759449"], "description"=>"<p>C9 (3 µg) was pre-mixed with various amounts of r<i>Ts</i>-Pmy (Lane 2, 0 µg; Lane 6, 10 µg; Lane 7, 2.5 µg and Lane 8, 1 µg) or r<i>Ts</i>87 (Lane 3, 10 µg; Lane 4, 2.5 µg) and incubated with 50 µM ZnCl<sub>2</sub>. Lane 5, blank C9 without adding Zn<sup>2+</sup> as a negative control. Lane 1, standard protein marker. The reaction mixtures were analyzed by SDS-PAGE and Western blot with anti-C9 mAb for Lane 8 as shown in Lane 9.</p>", "links"=>[], "tags"=>["c9", "polymerization"], "article_id"=>429819, "categories"=>["Infectious Diseases"], "users"=>["Zhifei Zhang", "Jing Yang", "Junfei Wei", "Yaping Yang", "Xiaoqin Chen", "Xi Zhao", "Yuan Gu", "Shijuan Cui", "Xinping Zhu"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0001225.g007", "stats"=>{"downloads"=>1, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Inhibition_of_Zn_2_induced_C9_polymerization_by_r_Ts_Pmy_/429819", "title"=>"Inhibition of Zn<sup>2+</sup>-induced C9 polymerization by r<i>Ts</i>-Pmy.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 17:00:03"}
  • {"files"=>["https://ndownloader.figshare.com/files/759390"], "description"=>"<p>BSA (Lane 2) and r<i>Ts</i>-Pmy (Lane 3) were transferred to a PVDF membrane, incubated with soluble C9 (left) or PBS only (right) and detected with monoclonal anti-C9 antibody.</p>", "links"=>[], "tags"=>["soluble", "c9", "immobilized"], "article_id"=>429759, "categories"=>["Infectious Diseases"], "users"=>["Zhifei Zhang", "Jing Yang", "Junfei Wei", "Yaping Yang", "Xiaoqin Chen", "Xi Zhao", "Yuan Gu", "Shijuan Cui", "Xinping Zhu"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0001225.g006", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Binding_of_soluble_C9_to_immobilized_r_Ts_Pmy_/429759", "title"=>"Binding of soluble C9 to immobilized r<i>Ts</i>-Pmy.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 16:59:44"}

PMC Usage Stats | Further Information

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Relative Metric

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