Potentiating Effects of MPL on DSPC Bearing Cationic Liposomes Promote Recombinant GP63 Vaccine Efficacy: High Immunogenicity and Protection
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Potentiating effects of MPL on DSPC bearing cationic liposomes promote recombinant GP63 vaccine efficacy: High immunogenicity and protection", "type"=>"journal", "authors"=>[{"first_name"=>"Saumyabrata", "last_name"=>"Mazumder", "scopus_author_id"=>"35942113100"}, {"first_name"=>"Mithun", "last_name"=>"Maji", "scopus_author_id"=>"38061715400"}, {"first_name"=>"Nahid", "last_name"=>"Ali", "scopus_author_id"=>"35568406500"}], "year"=>2011, "source"=>"PLoS Neglected Tropical Diseases", "identifiers"=>{"scopus"=>"2-s2.0-84855272530", "sgr"=>"84855272530", "issn"=>"19352727", "doi"=>"10.1371/journal.pntd.0001429", "pmid"=>"22206029", "isbn"=>"1935-2735 (Electronic)\\r1935-2727 (Linking)", "pui"=>"364024630"}, "id"=>"18e2dbc7-ee08-3eb4-aaa7-2669fb45a391", "abstract"=>"BACKGROUND: Vaccines that activate strong specific Th1-predominant immune responses are critically needed for many intracellular pathogens, including Leishmania. The requirement for sustained and efficient vaccination against leishmaniasis is to formulate the best combination of immunopotentiating adjuvant with the stable antigen (Ag) delivery system. The aim of the present study is to evaluate the effectiveness of an immunomodulator on liposomal Ag through subcutaneous (s.c.) route of immunization, and its usefulness during prime/boost against visceral leishmaniasis (VL) in BALB/c mice.\\n\\nMETHODOLOGY/PRINCIPAL FINDINGS: Towards this goal, we formulated recombinant GP63 (rGP63)-based vaccines either with monophosphoryl lipid A-trehalose dicorynomycolate (MPL-TDM) or entrapped within cationic liposomes or both. Combinatorial administration of liposomes with MPL-TDM during prime confers activation of dendritic cells, and induces an early robust T cell response. To investigate whether the combined formulation is required for optimum immune response during boost as well, we chose to evaluate the vaccine efficacy in mice primed with combined adjuvant system followed by boosting with either rGP63 alone, in association with MPL-TDM, liposomes or both. We provide evidences that the presence of either liposomal rGP63 or combined formulations during boost is necessary for effective Th1 immune responses (IFN-γ, IL-12, NO) before challenge infection. However, boosting with MPL-TDM in conjugation with liposomal rGP63 resulted in a greater number of IFN-γ producing effector T cells, significantly higher levels of splenocyte proliferation, and Th1 responses compared to mice boosted with liposomal rGP63, after virulent Leishmania donovani (L. donovani) challenge. Moreover, combined formulations offered superior protection against intracellular amastigote replication in macrophages in vitro, and hepatic and splenic parasite load in vivo.\\n\\nCONCLUSION: Our results define the immunopotentiating effect of MPL-TDM on protein Ag encapsulated in a controlled release system against experimental VL.", "link"=>"http://www.mendeley.com/research/potentiating-effects-mpl-dspc-bearing-cationic-liposomes-promote-recombinant-gp63-vaccine-efficacy-h", "reader_count"=>28, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Researcher"=>9, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>1, "Student > Master"=>4, "Student > Bachelor"=>3, "Professor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Researcher"=>9, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>1, "Student > Master"=>4, "Student > Bachelor"=>3, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>17, "Medicine and Dentistry"=>2, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Business, Management and Accounting"=>1, "Chemistry"=>1, "Immunology and Microbiology"=>3}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Chemistry"=>{"Chemistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>17}, "Business, Management and Accounting"=>{"Business, Management and Accounting"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>2}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"Brazil"=>1, "India"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84855272530"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84855272530?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84855272530&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84855272530&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84855272530", "dc:identifier"=>"SCOPUS_ID:84855272530", "eid"=>"2-s2.0-84855272530", "dc:title"=>"Potentiating effects of MPL on DSPC bearing cationic liposomes promote recombinant GP63 vaccine efficacy: High immunogenicity and protection", "dc:creator"=>"Mazumder S.", "prism:publicationName"=>"PLoS Neglected Tropical Diseases", "prism:issn"=>"19352727", "prism:eIssn"=>"19352735", "prism:volume"=>"5", "prism:issueIdentifier"=>"12", "prism:pageRange"=>nil, "prism:coverDate"=>"2011-12-01", "prism:coverDisplayDate"=>"December 2011", "prism:doi"=>"10.1371/journal.pntd.0001429", "citedby-count"=>"32", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Biozentrum, Universität Basel", "affiliation-city"=>"Basel", "affiliation-country"=>"Switzerland"}, {"@_fa"=>"true", "affilname"=>"Indian Institute of Chemical Biology", "affiliation-city"=>"Kolkata", "affiliation-country"=>"India"}], "pubmed-id"=>"22206029", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e1429", "source-id"=>"11900154404", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosntds%2Farticle%3Fid%3D10.1371%252Fjournal.pntd.0001429", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"25", "xml_views"=>"12", "html_views"=>"275"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"100", "xml_views"=>"73", "html_views"=>"1295"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"12", "xml_views"=>"5", "html_views"=>"23"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"37"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"6", "html_views"=>"41"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"90"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"50"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"36"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"18"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"15"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"18"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"78"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"19"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"2"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"3", "html_views"=>"0"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"0"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"4"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/355852", "https://ndownloader.figshare.com/files/355944", "https://ndownloader.figshare.com/files/356019"], "description"=>"<div><h3>Background</h3><p>Vaccines that activate strong specific Th1-predominant immune responses are critically needed for many intracellular pathogens, including <em>Leishmania</em>. The requirement for sustained and efficient vaccination against leishmaniasis is to formulate the best combination of immunopotentiating adjuvant with the stable antigen (Ag) delivery system. The aim of the present study is to evaluate the effectiveness of an immunomodulator on liposomal Ag through subcutaneous (s.c.) route of immunization, and its usefulness during prime/boost against visceral leishmaniasis (VL) in BALB/c mice.</p> <h3>Methodology/Principal Findings</h3><p>Towards this goal, we formulated recombinant GP63 (rGP63)-based vaccines either with monophosphoryl lipid A-trehalose dicorynomycolate (MPL-TDM) or entrapped within cationic liposomes or both. Combinatorial administration of liposomes with MPL-TDM during prime confers activation of dendritic cells, and induces an early robust T cell response. To investigate whether the combined formulation is required for optimum immune response during boost as well, we chose to evaluate the vaccine efficacy in mice primed with combined adjuvant system followed by boosting with either rGP63 alone, in association with MPL-TDM, liposomes or both. We provide evidences that the presence of either liposomal rGP63 or combined formulations during boost is necessary for effective Th1 immune responses (IFN-γ, IL-12, NO) before challenge infection. However, boosting with MPL-TDM in conjugation with liposomal rGP63 resulted in a greater number of IFN-γ producing effector T cells, significantly higher levels of splenocyte proliferation, and Th1 responses compared to mice boosted with liposomal rGP63, after virulent <em>Leishmania donovani</em> (<em>L. donovani</em>) challenge. Moreover, combined formulations offered superior protection against intracellular amastigote replication in macrophages in vitro, and hepatic and splenic parasite load in vivo.</p> <h3>Conclusion</h3><p>Our results define the immunopotentiating effect of MPL-TDM on protein Ag encapsulated in a controlled release system against experimental VL.</p> </div>", "links"=>[], "tags"=>["potentiating", "effects", "mpl", "dspc", "bearing", "cationic", "liposomes", "recombinant", "gp63", "immunogenicity"], "article_id"=>130314, "categories"=>["Immunology"], "users"=>["Saumyabrata Mazumder", "Mithun Maji", "Nahid Ali"], "doi"=>["https://dx.doi.org/10.1371/journal.pntd.0001429.s001", "https://dx.doi.org/10.1371/journal.pntd.0001429.s002", "https://dx.doi.org/10.1371/journal.pntd.0001429.s003"], "stats"=>{"downloads"=>4, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Potentiating_Effects_of_MPL_on_DSPC_Bearing_Cationic_Liposomes_Promote_Recombinant_GP63_Vaccine_Efficacy_High_Immunogenicity_and_Protection/130314", "title"=>"Potentiating Effects of MPL on DSPC Bearing Cationic Liposomes Promote Recombinant GP63 Vaccine Efficacy: High Immunogenicity and Protection", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2011-12-20 00:05:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/700341"], "description"=>"<p>BALB/c mice primed with liposomal rGP63 plus MPL-TDM, followed by boosted with either rGP63 alone, in association with MPL-TDM, entrapped within liposomes, or both before <i>L. donovani</i> infection. rGP63-specific (A) IFN-γ, (B) IL-12 (p40), (C) NO, (D) IL-4, and (E) IL-10 were measured from cultured supernatants of splenocytes from vaccinated mice. The results are mean ± S.E. of five individual mice per group, representative of two independent experiments with similar results. *<i>p</i><0.05, ***<i>p</i><0.001 assessed by one-way ANOVA and Tukey's multiple comparison tests, compared to PBS, unless stated.</p>", "links"=>[], "tags"=>["splenocytes", "mice", "vaccinated"], "article_id"=>370723, "categories"=>["Immunology"], "users"=>["Saumyabrata Mazumder", "Mithun Maji", "Nahid Ali"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0001429.g003", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cytokine_and_NO_production_in_splenocytes_from_mice_vaccinated_with_different_prime_boost_regimens_/370723", "title"=>"Cytokine and NO production in splenocytes from mice vaccinated with different prime/boost regimens.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-12-20 00:12:03"}
  • {"files"=>["https://ndownloader.figshare.com/files/700250"], "description"=>"<p>A. Bone-marrow derived DCs were isolated and stimulated with LPS (1 µg/ml), liposomes (50 µM), MPL-TDM (100 ng/ml) and combination of liposomes (50 µM) and MPL-TDM (100 ng/ml) on activation of BMDCs. The release of IL-12 (p40) (A) and NO (B) were measured subsequently. The results are duplicate mean ± S.E, representative of three independent experiments with similar results. *<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001 assessed by two-tail t test. C. Ag-presenting capacity of CD11c<sup>+</sup> and CD11c<sup>−</sup> cells isolated from mice immunized with PBS, MPL-TDM plus liposomes, and liposomal rGP63 plus MPL-TDM. CD11c<sup>+</sup> and CD11c<sup>−</sup> cells were isolated from LN as described in <a href=\"http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001429#s2\" target=\"_blank\">Materials and Methods</a> and IL-2 content was assayed through ELISA.</p>", "links"=>[], "tags"=>["dcs"], "article_id"=>370639, "categories"=>["Immunology"], "users"=>["Saumyabrata Mazumder", "Mithun Maji", "Nahid Ali"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0001429.g002", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Activation_of_DCs_in_the_generation_of_immune_responses_/370639", "title"=>"Activation of DCs in the generation of immune responses.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-12-20 00:10:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/700573"], "description"=>"<p>Quantification of single viable cell was determined by limiting dilution assay performed 3 months after infection on the cells isolated from liver (A) and spleen (B). <a href=\"http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001429#s3\" target=\"_blank\">Results</a> were expressed as log of total organ parasite burden. Data represent the mean ± S.E of five individual mice per group, representative of two independent experiments with similar results. *<i>p</i><0.05, ** <i>p</i><0.01 as assessed by one-way ANOVA and Tukey's multiple comparison test.</p>", "links"=>[], "tags"=>["mice", "vaccinated"], "article_id"=>370958, "categories"=>["Immunology"], "users"=>["Saumyabrata Mazumder", "Mithun Maji", "Nahid Ali"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0001429.g006", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Evaluation_of_protection_against_L_donovani_challenge_in_mice_vaccinated_with_different_vaccine_regimens_/370958", "title"=>"Evaluation of protection against <i>L. donovani</i> challenge in mice vaccinated with different vaccine regimens.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-12-20 00:15:58"}
  • {"files"=>["https://ndownloader.figshare.com/files/700734"], "description"=>"<p>Mice were primed with liposomal rGP63 plus MPL-TDM, followed by boosting with either rGP63 alone, in association with MPL-TDM, entrapped within liposomes, or both after challenge with <i>L. donovani</i>. Splenocytes were isolated from vaccinated mice at 3 months after challenge infection and the expression of IFN-γ producing CD4<sup>+</sup> (A) and CD8<sup>+</sup> (B) T was studied in the presence of rGP63 (5 µg/ml). Data represent the mean of triplicate wells ± SE of three individual mice per group. *<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001 assessed by Student's two-tail <i>t</i> test.</p>", "links"=>[], "tags"=>["cytometric", "rgp63-specific", "producing", "cells"], "article_id"=>371114, "categories"=>["Immunology"], "users"=>["Saumyabrata Mazumder", "Mithun Maji", "Nahid Ali"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0001429.g008", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Flow_cytometric_analysis_of_rGP63_specific_IFN_producing_CD4_CD8_T_cells_after_challenge_infection_/371114", "title"=>"Flow cytometric analysis of rGP63-specific IFN-γ producing CD4<sup>+</sup> CD8<sup>+</sup> T cells after challenge infection.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-12-20 00:18:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/700444"], "description"=>"<p>BALB/c mice were primed with liposomal rGP63 plus MPL-TDM, followed by boosting with either rGP63 alone, in association with MPL-TDM, or liposomes, or both before challenge infection. Splenocytes were isolated from vaccinated mice and the expression of IFN-γ producing CD4<sup>+</sup> (A) and CD8<sup>+</sup> T (B) was studied in the presence of rGP63 (5 µg/ml). Data represent the mean of triplicate wells ± SE of three individual mice per group. **<i>p</i><0.01, ***<i>p</i><0.001 assessed by two-tail <i>t</i> test.</p>", "links"=>[], "tags"=>["cytometric", "rgp63-specific", "producing"], "article_id"=>370826, "categories"=>["Immunology"], "users"=>["Saumyabrata Mazumder", "Mithun Maji", "Nahid Ali"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0001429.g004", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Flow_cytometric_analysis_of_rGP63_specific_IFN_producing_CD4_and_CD8_T_cells_/370826", "title"=>"Flow cytometric analysis of rGP63-specific IFN-γ producing CD4<sup>+</sup> and CD8<sup>+</sup> T cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-12-20 00:13:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/700653"], "description"=>"<p>BALB/c mice were primed with liposomal rGP63 plus MPL-TDM, followed by boosted with either rGP63 alone, in association with MPL-TDM, entrapped within liposomes, or both after challenge with <i>L. donovani</i>. The cultured supernatants of splenocytes were collected, and assayed for (A) IFN-γ, (B) IL-12 (p40), (C) NO, (D) IL-4, and (E) IL-10. The results are shown as the mean absorbance values ± S.E of five individual animals per group, representative of two independent experiments with similar results. *<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001 assessed by one-way ANOVA and Tukey's multiple comparison tests, compared to PBS, unless stated.</p>", "links"=>[], "tags"=>["mice", "vaccinated", "regimens", "months", "post-infection"], "article_id"=>371031, "categories"=>["Immunology"], "users"=>["Saumyabrata Mazumder", "Mithun Maji", "Nahid Ali"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0001429.g007", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cytokine_and_NO_production_in_mice_vaccinated_with_different_prime_boost_regimens_3_months_post_infection_with_L_donovani_/371031", "title"=>"Cytokine and NO production in mice vaccinated with different prime/boost regimens 3 months post-infection with <i>L. donovani</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-12-20 00:17:11"}
  • {"files"=>["https://ndownloader.figshare.com/files/700496"], "description"=>"<p>Peritoneal macrophages were isolated from different groups of vaccinated mice followed by in vitro infection with virulent <i>L. donovani</i>. The cells were stained with Giemsa for determination of intracellular parasite numbers (A). Culture supernatants were removed at 72 h and IL-12 (p40) (B), and the NO (C) production in the medium were estimated. Data represent mean ± SE of three individual mice per group. *<i>p</i><0.05, ***<i>p</i><0.001 assessed by Student's two-tail <i>t</i> test.</p>", "links"=>[], "tags"=>["macrophage", "activation", "suppress"], "article_id"=>370884, "categories"=>["Immunology"], "users"=>["Saumyabrata Mazumder", "Mithun Maji", "Nahid Ali"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0001429.g005", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Determination_of_macrophage_activation_to_suppress_L_donovani_infection_/370884", "title"=>"Determination of macrophage activation to suppress <i>L. donovani</i> infection.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-12-20 00:14:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/700149"], "description"=>"<p>DTH and rGP63-specific cytokine analysis in mice vaccinated with either rGP63 alone, or in association with MPL-TDM, or DSPC-cationic liposomes, or both 3 weeks after final immunization. A. DTH response was determined as described in the <a href=\"http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001429#s2\" target=\"_blank\">Materials and Methods</a>. Spleens were collected, splenocytes were restimulated in vitro for 72 h with 2.5 µg/ml of rGP63 and levels of (B) IFN-γ, (C) IL-12(p40), and (D) IL-4 were measured. All the results are mean ± S.E. of five individual mice per group, representative of two independent experiments with similar results. ***<i>p</i><0.001 assessed by one-way ANOVA and Tukey's multiple comparison tests, compared to PBS, unless stated.</p>", "links"=>[], "tags"=>["dth", "cytokine", "liposomal", "rgp63", "mpl-tdm", "vaccinated"], "article_id"=>370527, "categories"=>["Immunology"], "users"=>["Saumyabrata Mazumder", "Mithun Maji", "Nahid Ali"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0001429.g001", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Induction_of_DTH_and_cytokine_response_in_liposomal_rGP63_plus_MPL_TDM_vaccinated_mice_/370527", "title"=>"Induction of DTH and cytokine response in liposomal rGP63 plus MPL-TDM vaccinated mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-12-20 00:08:47"}

PMC Usage Stats | Further Information

  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"58", "unique-ip"=>"48", "pdf"=>"41", "year"=>"2012", "figure"=>"6", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"41", "year"=>"2012", "pdf"=>"28", "unique-ip"=>"37", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"42", "unique-ip"=>"40", "pdf"=>"28", "year"=>"2012", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"24", "year"=>"2012", "pdf"=>"12", "unique-ip"=>"22", "figure"=>"12", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"22", "unique-ip"=>"24", "pdf"=>"13", "year"=>"2012", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"20", "year"=>"2012", "pdf"=>"20", "unique-ip"=>"22", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"24", "full-text"=>"26", "pdf"=>"14", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"18", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"26", "full-text"=>"17", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"18", "full-text"=>"17", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"18", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"24", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"4", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"6", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"2", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"5", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"14", "full-text"=>"7", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"5", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}

Relative Metric

{"start_date"=>"2011-01-01T00:00:00Z", "end_date"=>"2011-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[304, 568, 702, 818, 927, 1027, 1118, 1206, 1285, 1357, 1427, 1500, 1564, 1636, 1705, 1773, 1840, 1909, 1974, 2039, 2106, 2170, 2234, 2296, 2358, 2423, 2484, 2546, 2606, 2673, 2734, 2795, 2857, 2921, 2984, 3046, 3100]}, {"subject_area"=>"/Biology and life sciences/Immunology", "average_usage"=>[303, 577, 713, 831, 950, 1048, 1144, 1230, 1307, 1381, 1454, 1521, 1590, 1655, 1713, 1778, 1848, 1913, 1972, 2033, 2098, 2154, 2215, 2263, 2322, 2388, 2436, 2500, 2571, 2623, 2689, 2754, 2806, 2864, 2930, 2984, 3038]}, {"subject_area"=>"/Biology and life sciences/Organisms", "average_usage"=>[316, 571, 699, 812, 915, 1008, 1095, 1180, 1255, 1319, 1393, 1461, 1528, 1595, 1662, 1729, 1802, 1868, 1937, 1997, 2062, 2130, 2192, 2254, 2324, 2386, 2450, 2510, 2579, 2642, 2716, 2778, 2842, 2908, 2979, 3042, 3095]}, {"subject_area"=>"/Biology and life sciences/Veterinary science", "average_usage"=>[362, 655, 777, 893, 988, 1083, 1166, 1276, 1344, 1406, 1458, 1525, 1576, 1632, 1706, 1786, 1854, 1906, 1942, 1993, 2058, 2126, 2184, 2259, 2320, 2380, 2463, 2522, 2570, 2692, 2747, 2804, 2875, 2963, 3005, 3054, 3104]}, {"subject_area"=>"/Medicine and health sciences/Immunology", "average_usage"=>[299, 582, 725, 844, 971, 1076, 1167, 1253, 1328, 1403, 1480, 1551, 1620, 1691, 1756, 1821, 1893, 1962, 2032, 2110, 2178, 2236, 2300, 2358, 2419, 2465, 2529, 2587, 2643, 2700, 2756, 2817, 2869, 2932, 2986, 3046, 3101]}, {"subject_area"=>"/Medicine and health sciences/Parasitic diseases", "average_usage"=>[362, 736, 861, 965, 1072, 1194, 1301, 1377, 1465, 1535, 1624, 1694, 1745, 1817, 1877, 1958, 2038, 2106, 2167, 2241, 2288, 2381, 2447, 2508, 2566, 2622, 2687, 2781, 2853, 2913, 2984, 3057, 3141, 3186, 3250, 3302, 3367]}, {"subject_area"=>"/Medicine and health sciences/Public and occupational health", "average_usage"=>[343, 648, 793, 906, 1017, 1126, 1226, 1298, 1386, 1458, 1536, 1617, 1694, 1771, 1851, 1915, 1982, 2042, 2111, 2171, 2220, 2273, 2339, 2430, 2509, 2572, 2640, 2695, 2766, 2822, 2882, 2956, 2992, 3046, 3082, 3151, 3206]}, {"subject_area"=>"/Medicine and health sciences/Tropical diseases", "average_usage"=>[338, 662, 813, 915, 1030, 1125, 1242, 1315, 1390, 1447, 1525, 1593, 1653, 1750, 1827, 1887, 1972, 2030, 2103, 2153, 2215, 2279, 2336, 2397, 2468, 2528, 2590, 2670, 2746, 2820, 2871, 2936, 2986, 3043, 3080, 3133, 3170]}]}
Loading … Spinner
There are currently no alerts