Rapid Molecular Assays for the Detection of Yellow Fever Virus in Low-Resource Settings
Publication Date
March 06, 2014
Journal
PLOS Neglected Tropical Diseases
Authors
Camille Escadafal, Oumar Faye, Amadou Alpha Sall, Ousmane Faye, et al
Volume
8
Issue
3
Pages
e2730
DOI
https://dx.plos.org/10.1371/journal.pntd.0002730
Publisher URL
http://journals.plos.org/plosntds/article?id=10.1371%2Fjournal.pntd.0002730
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/24603874
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945292
Europe PMC
http://europepmc.org/abstract/MED/24603874
Web of Science
000337348800014
Scopus
84897465271
Mendeley
http://www.mendeley.com/research/rapid-molecular-assays-detection-yellow-fever-virus-lowresource-settings-2
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Mendeley | Further Information

{"title"=>"Rapid Molecular Assays for the Detection of Yellow Fever Virus in Low-Resource Settings", "type"=>"journal", "authors"=>[{"first_name"=>"Camille", "last_name"=>"Escadafal", "scopus_author_id"=>"54079526400"}, {"first_name"=>"Oumar", "last_name"=>"Faye", "scopus_author_id"=>"24479406600"}, {"first_name"=>"Amadou Alpha", "last_name"=>"Sall", "scopus_author_id"=>"6701512394"}, {"first_name"=>"Ousmane", "last_name"=>"Faye", "scopus_author_id"=>"7006739058"}, {"first_name"=>"Manfred", "last_name"=>"Weidmann", "scopus_author_id"=>"7004711811"}, {"first_name"=>"Oliver", "last_name"=>"Strohmeier", "scopus_author_id"=>"6506633513"}, {"first_name"=>"Felix", "last_name"=>"von Stetten", "scopus_author_id"=>"35231473800"}, {"first_name"=>"Josef", "last_name"=>"Drexler", "scopus_author_id"=>"55418499700"}, {"first_name"=>"Michael", "last_name"=>"Eberhard", "scopus_author_id"=>"57196909949"}, {"first_name"=>"Matthias", "last_name"=>"Niedrig", "scopus_author_id"=>"7003827932"}, {"first_name"=>"Pranav", "last_name"=>"Patel", "scopus_author_id"=>"56408906800"}], "year"=>2014, "source"=>"PLoS Neglected Tropical Diseases", "identifiers"=>{"sgr"=>"84897465271", "isbn"=>"1935-2735 (Electronic)\\r1935-2727 (Linking)", "pui"=>"372738293", "issn"=>"19352735", "doi"=>"10.1371/journal.pntd.0002730", "scopus"=>"2-s2.0-84897465271", "pmid"=>"24603874"}, "id"=>"a0d1bdff-efc3-3040-81c7-66c9b9431982", "abstract"=>"BACKGROUND: Yellow fever (YF) is an acute viral hemorrhagic disease transmitted by Aedes mosquitoes. The causative agent, the yellow fever virus (YFV), is found in tropical and subtropical areas of South America and Africa. Although a vaccine is available since the 1930s, YF still causes thousands of deaths and several outbreaks have recently occurred in Africa. Therefore, rapid and reliable diagnostic methods easy to perform in low-resources settings could have a major impact on early detection of outbreaks and implementation of appropriate response strategies such as vaccination and/or vector control.\\n\\nMETHODOLOGY: The aim of this study was to develop a YFV nucleic acid detection method applicable in outbreak investigations and surveillance studies in low-resource and field settings. The method should be simple, robust, rapid and reliable. Therefore, we adopted an isothermal approach and developed a recombinase polymerase amplification (RPA) assay which can be performed with a small portable instrument and easy-to-use lyophilized reagents. The assay was developed in three different formats (real-time with or without microfluidic semi-automated system and lateral-flow assay) to evaluate their application for different purposes. Analytical specificity and sensitivity were evaluated with a wide panel of viruses and serial dilutions of YFV RNA. Mosquito pools and spiked human plasma samples were also tested for assay validation. Finally, real-time RPA in portable format was tested under field conditions in Senegal.\\n\\nCONCLUSION/SIGNIFICANCE: The assay was able to detect 20 different YFV strains and demonstrated no cross-reactions with closely related viruses. The RPA assay proved to be a robust, portable method with a low detection limit (<21 genome equivalent copies per reaction) and rapid processing time (<20 min). Results from real-time RPA field testing were comparable to results obtained in the laboratory, thus confirming our method is suitable for YFV detection in low-resource settings.", "link"=>"http://www.mendeley.com/research/rapid-molecular-assays-detection-yellow-fever-virus-lowresource-settings-2", "reader_count"=>87, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>4, "Librarian"=>1, "Researcher"=>23, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>17, "Student > Postgraduate"=>4, "Student > Master"=>16, "Other"=>3, "Student > Bachelor"=>12, "Lecturer"=>1, "Professor"=>4}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>4, "Librarian"=>1, "Researcher"=>23, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>17, "Student > Postgraduate"=>4, "Student > Master"=>16, "Other"=>3, "Student > Bachelor"=>12, "Lecturer"=>1, "Professor"=>4}, "reader_count_by_subject_area"=>{"Engineering"=>10, "Unspecified"=>3, "Biochemistry, Genetics and Molecular Biology"=>11, "Nursing and Health Professions"=>2, "Agricultural and Biological Sciences"=>36, "Medicine and Dentistry"=>10, "Arts and Humanities"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>2, "Veterinary Science and Veterinary Medicine"=>2, "Chemistry"=>4, "Social Sciences"=>2, "Immunology and Microbiology"=>4}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>10}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>10}, "Chemistry"=>{"Chemistry"=>4}, "Social Sciences"=>{"Social Sciences"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>4}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>36}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>2}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>11}, "Unspecified"=>{"Unspecified"=>3}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>2}, "Arts and Humanities"=>{"Arts and Humanities"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>2}}, "reader_count_by_country"=>{"Sweden"=>1, "United States"=>2, "Japan"=>1, "United Kingdom"=>2, "Chile"=>1, "Kenya"=>1, "Nigeria"=>1}, "group_count"=>8}

CrossRef

Scopus | Further Information

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  • {"files"=>["https://ndownloader.figshare.com/files/1410470"], "description"=>"<p>PPV: positive predictive value; NPV: negative predictive value.</p>", "links"=>[], "tags"=>["microbiology", "Virology", "Emerging viral diseases", "Viral disease diagnosis", "Applied microbiology", "Real-time", "rt-rpa", "assay", "scanner", "geneslice", "cartridge", "detecting", "yfv", "mosquito"], "article_id"=>954244, "categories"=>["Biological Sciences"], "users"=>["Camille Escadafal", "Oumar Faye", "Amadou Alpha Sall", "Ousmane Faye", "Manfred Weidmann", "Oliver Strohmeier", "Felix von Stetten", "Josef Drexler", "Michael Eberhard", "Matthias Niedrig", "Pranav Patel"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0002730.t004", "stats"=>{"downloads"=>3, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Performance_of_the_real_time_RT_RPA_assay_using_the_Tube_Scanner_or_the_GeneSlice_cartridge_in_comparison_to_the_reference_method_real_time_RT_PCR_for_detecting_YFV_in_mosquito_pools_/954244", "title"=>"Performance of the real-time RT-RPA assay using the Tube Scanner or the GeneSlice cartridge in comparison to the reference method, real-time RT-PCR, for detecting YFV in mosquito pools.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-03-06 04:04:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1410467"], "description"=>"<p>Ct: cycle threshold; Tt: time threshold; Neg.: Negative; Pos.: Positive; Undet.: Undetermined.</p>", "links"=>[], "tags"=>["microbiology", "Virology", "Emerging viral diseases", "Viral disease diagnosis", "Applied microbiology", "Real-time", "lfs", "rt-rpa", "yfv", "supernatant", "plasma", "spiked", "rt-pcr"], "article_id"=>954241, "categories"=>["Biological Sciences"], "users"=>["Camille Escadafal", "Oumar Faye", "Amadou Alpha Sall", "Ousmane Faye", "Manfred Weidmann", "Oliver Strohmeier", "Felix von Stetten", "Josef Drexler", "Michael Eberhard", "Matthias Niedrig", "Pranav Patel"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0002730.g002", "stats"=>{"downloads"=>0, "page_views"=>22, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sensitivity_testing_of_the_real_time_and_LFS_RT_RPA_with_YFV_cell_supernatant_and_human_plasma_spiked_with_YFV_in_comparison_with_real_time_RT_PCR_results_/954241", "title"=>"Sensitivity testing of the real-time and LFS RT-RPA with YFV cell supernatant and human plasma spiked with YFV in comparison with real-time RT-PCR results.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-06 04:04:31"}

PMC Usage Stats | Further Information

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Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[291]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[292, 461]}, {"subject_area"=>"/Engineering and technology", "average_usage"=>[282]}, {"subject_area"=>"/Engineering and technology/Fluidics", "average_usage"=>[317, 522]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[285]}, {"subject_area"=>"/People and places", "average_usage"=>[302]}]}
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