Transient Loss of Protection Afforded by a Live Attenuated Non-typhoidal Salmonella Vaccine in Mice Co-infected with Malaria
Publication Date
September 14, 2015
Journal
PLOS Neglected Tropical Diseases
Authors
Jason P. Mooney, Seung Joo Lee, Kristen L. Lokken, Minelva R. Nanton, et al
Volume
9
Issue
9
Pages
e0004027
DOI
https://dx.plos.org/10.1371/journal.pntd.0004027
Publisher URL
http://journals.plos.org/plosntds/article?id=10.1371%2Fjournal.pntd.0004027
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/26366739
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4569369
Europe PMC
http://europepmc.org/abstract/MED/26366739
Web of Science
000363031200021
Scopus
84943196924
Mendeley
http://www.mendeley.com/research/transient-loss-protection-afforded-live-attenuated-nontyphoidal-salmonella-vaccine-mice-coinfected-m
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Transient Loss of Protection Afforded by a Live Attenuated Non-typhoidal Salmonella Vaccine in Mice Co-infected with Malaria", "type"=>"journal", "authors"=>[{"first_name"=>"Jason P.", "last_name"=>"Mooney", "scopus_author_id"=>"56004414800"}, {"first_name"=>"Seung Joo", "last_name"=>"Lee", "scopus_author_id"=>"55028755300"}, {"first_name"=>"Kristen L.", "last_name"=>"Lokken", "scopus_author_id"=>"56004505000"}, {"first_name"=>"Minelva R.", "last_name"=>"Nanton", "scopus_author_id"=>"26655951500"}, {"first_name"=>"Sean Paul", "last_name"=>"Nuccio", "scopus_author_id"=>"17346730200"}, {"first_name"=>"Stephen J.", "last_name"=>"McSorley", "scopus_author_id"=>"6603810635"}, {"first_name"=>"Renée M.", "last_name"=>"Tsolis", "scopus_author_id"=>"7003616130"}], "year"=>2015, "source"=>"PLoS Neglected Tropical Diseases", "identifiers"=>{"sgr"=>"84943196924", "doi"=>"10.1371/journal.pntd.0004027", "isbn"=>"1935-2727", "pmid"=>"26366739", "issn"=>"19352735", "scopus"=>"2-s2.0-84943196924", "pui"=>"606211349"}, "id"=>"1afaf1f4-2b86-35a8-9e7d-177c00454314", "abstract"=>"In immunocompetent individuals, non-typhoidal Salmonella serovars (NTS) are associated with gastroenteritis, however, there is currently an epidemic of NTS bloodstream infections in sub-Saharan Africa. Plasmodium falciparummalaria is an important risk factor for inva- sive NTS bloodstream in African children. Here we investigated whether a live, attenuated Salmonella vaccine could be protective in mice, in the setting of concurrent malaria. Surpris- ingly, mice acutely infected with the nonlethalmalaria parasite Plasmodium yoelii 17XNL exhibited a profound loss of protective immunity to NTS, but vaccine-mediated protection was restored after resolution ofmalaria. Absence of protective immunity during acute malaria correlated with maintenance of antibodies to NTS, but a marked reduction in effec- tor capability of Salmonella-specific CD4 and CD8 T cells. Further, increased expression of the inhibitory molecule PD1 was identified on memory CD4 T cells induced by vaccination. Blockade of IL-10 restored protection against S. Typhimurium, without restoring CD4 T cell effector function. Simultaneous blockade of CTLA-4, LAG3, and PDL1 restored IFN-γ pro- duction by vaccine-induced memory CD4 T cells but was not sufficient to restore protection. Together, these data demonstrate that malaria parasite infection induces a temporary loss of an established adaptive immune response via multiple mechanisms, and suggest that in the setting of acute malaria, protection against NTS mediated by live vaccines may be interrupted.", "link"=>"http://www.mendeley.com/research/transient-loss-protection-afforded-live-attenuated-nontyphoidal-salmonella-vaccine-mice-coinfected-m", "reader_count"=>38, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Librarian"=>1, "Researcher"=>4, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>10, "Student > Master"=>5, "Student > Bachelor"=>12, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Librarian"=>1, "Researcher"=>4, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>10, "Student > Master"=>5, "Student > Bachelor"=>12, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Nursing and Health Professions"=>2, "Agricultural and Biological Sciences"=>6, "Medicine and Dentistry"=>23, "Psychology"=>1, "Computer Science"=>1, "Immunology and Microbiology"=>3}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>23}, "Psychology"=>{"Psychology"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>6}, "Computer Science"=>{"Computer Science"=>1}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>2}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"United States"=>1, "Tanzania"=>1}, "group_count"=>1}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84943196924"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84943196924?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84943196924&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84943196924&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84943196924", "dc:identifier"=>"SCOPUS_ID:84943196924", "eid"=>"2-s2.0-84943196924", "dc:title"=>"Transient Loss of Protection Afforded by a Live Attenuated Non-typhoidal Salmonella Vaccine in Mice Co-infected with Malaria", "dc:creator"=>"Mooney J.", "prism:publicationName"=>"PLoS Neglected Tropical Diseases", "prism:issn"=>"19352727", "prism:eIssn"=>"19352735", "prism:volume"=>"9", "prism:issueIdentifier"=>"9", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-09-14", "prism:coverDisplayDate"=>"14 September 2015", "prism:doi"=>"10.1371/journal.pntd.0004027", "citedby-count"=>"12", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"University of California, Davis", "affiliation-city"=>"Davis", "affiliation-country"=>"United States"}], "pubmed-id"=>"26366739", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0004027", "source-id"=>"11900154404", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosntds%2Farticle%3Fid%3D10.1371%252Fjournal.pntd.0004027", "share_count"=>3, "like_count"=>0, "comment_count"=>1, "click_count"=>0, "total_count"=>4}

Counter

  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"50", "xml_views"=>"1", "html_views"=>"648"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"25", "xml_views"=>"2", "html_views"=>"227"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"19", "xml_views"=>"1", "html_views"=>"54"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"3", "html_views"=>"7"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"1"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"6"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"4"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"10", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"1"}
  • {"month"=>"11", "year"=>"2020", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"5"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2262270", "https://ndownloader.figshare.com/files/2262271", "https://ndownloader.figshare.com/files/2262272", "https://ndownloader.figshare.com/files/2262273", "https://ndownloader.figshare.com/files/2262274"], "description"=>"<div><p>In immunocompetent individuals, non-typhoidal <i>Salmonella</i> serovars (NTS) are associated with gastroenteritis, however, there is currently an epidemic of NTS bloodstream infections in sub-Saharan Africa. <i>Plasmodium falciparum</i> malaria is an important risk factor for invasive NTS bloodstream in African children. Here we investigated whether a live, attenuated <i>Salmonella</i> vaccine could be protective in mice, in the setting of concurrent malaria. Surprisingly, mice acutely infected with the nonlethal malaria parasite <i>Plasmodium yoelii</i> 17XNL exhibited a profound loss of protective immunity to NTS, but vaccine-mediated protection was restored after resolution of malaria. Absence of protective immunity during acute malaria correlated with maintenance of antibodies to NTS, but a marked reduction in effector capability of <i>Salmonella</i>-specific CD4 and CD8 T cells. Further, increased expression of the inhibitory molecule PD1 was identified on memory CD4 T cells induced by vaccination. Blockade of IL-10 restored protection against <i>S</i>. Typhimurium, without restoring CD4 T cell effector function. Simultaneous blockade of CTLA-4, LAG3, and PDL1 restored IFN-γ production by vaccine-induced memory CD4 T cells but was not sufficient to restore protection. Together, these data demonstrate that malaria parasite infection induces a temporary loss of an established adaptive immune response via multiple mechanisms, and suggest that in the setting of acute malaria, protection against NTS mediated by live vaccines may be interrupted.</p></div>", "links"=>[], "tags"=>["Transient Loss", "effector capability", "CTLA", "NTS bloodstream", "CD 8 T cells", "memory CD 4 T cells", "Plasmodium falciparum malaria", "lag", "ifn", "NTS bloodstream infections", "protection", "molecule PD 1", "malaria parasite Plasmodium yoelii 17 XNL", "CD 4 T cell effector function", "immunocompetent individuals", "attenuated Salmonella vaccine", "African children", "malaria parasite infection", "PDL 1", "Simultaneous blockade", "Protection Afforded", "risk factor", "il"], "article_id"=>1542208, "categories"=>["Uncategorised"], "users"=>["Jason P. Mooney", "Seung-Joo Lee", "Kristen L. Lokken", "Minelva R. Nanton", "Sean-Paul Nuccio", "Stephen J. McSorley", "Renée M. Tsolis"], "doi"=>["https://dx.doi.org/10.1371/journal.pntd.0004027.s001", "https://dx.doi.org/10.1371/journal.pntd.0004027.s002", "https://dx.doi.org/10.1371/journal.pntd.0004027.s003", "https://dx.doi.org/10.1371/journal.pntd.0004027.s004", "https://dx.doi.org/10.1371/journal.pntd.0004027.s005"], "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Transient_Loss_of_Protection_Afforded_by_a_Live_Attenuated_Non_typhoidal_Salmonella_Vaccine_in_Mice_Co_infected_with_Malaria/1542208", "title"=>"Transient Loss of Protection Afforded by a Live Attenuated Non-typhoidal <i>Salmonella Vaccine</i> in Mice Co-infected with Malaria", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-09-14 03:56:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/2262264"], "description"=>"<p>C57BL/6 mice were vaccinated with <i>S</i>. Typhimurium BRD509:2W1S (Vac) and then given <i>P</i>. <i>yoelii</i> (<i>P</i>.<i>y</i>.) as described above. (<b>A</b>) At 14 d post <i>P</i>. <i>yoelii</i> infection, splenic T cells were evaluated by flow cytometry for expression of CTLA-4 and PD1. Bar graphs show percentage of CTLA-4+, and PD1+ among 2W1S-specific CD4 T cells. Data are shown as Mean+SEM (n = 10). (<b>B-D</b>) <i>P</i>. <i>yoelii</i>-infected mice were given either an antibody cocktail consisting of αCTLA-4, αLAG3 and αPDL-1 or isotype control antibodies over 7 days as described in methods. (<b>B</b>) Parasite burden with blocking antibody cocktail (n = 3) or isotype control (n = 2), as determined by blood smears. (<b>C</b>) At 14 days post-<i>P</i>. <i>yoelii</i> infection, all the treated mice were injected i.v. with heat-killed <i>S</i>. Typhimurium lysates for 4 hours. Splenic CD4 T cells were analyzed by flow cytometry for IFN-γ production. Bar graphs show percentage of IFN-γ producing Tbet+ CD44+ 2W1S-specific CD4 T cells with blocking antibody cocktail (n = 3) or isotype control (n = 2). (<b>D</b>) Antibody-treated mice were challenged with 750 CFU of virulent <i>S</i>. Typhimurium via the i.v. route at 14 days after <i>P</i>. <i>yoelii</i> infection. At 3 d post-challenge, bacterial burden was determined in livers and normalized to tissue weight. Data represent Mean+SEM (n = 5 per group). Data are shown as Mean+SEM. Significance of differences between groups was determined using a Student’s <i>t</i> test (*, <i>p</i><0.05; ns, not significant).</p>", "links"=>[], "tags"=>["Transient Loss", "effector capability", "CTLA", "NTS bloodstream", "CD 8 T cells", "memory CD 4 T cells", "Plasmodium falciparum malaria", "lag", "ifn", "NTS bloodstream infections", "protection", "molecule PD 1", "malaria parasite Plasmodium yoelii 17 XNL", "CD 4 T cell effector function", "immunocompetent individuals", "attenuated Salmonella vaccine", "African children", "malaria parasite infection", "PDL 1", "Simultaneous blockade", "Protection Afforded", "risk factor", "il"], "article_id"=>1542202, "categories"=>["Uncategorised"], "users"=>["Jason P. Mooney", "Seung-Joo Lee", "Kristen L. Lokken", "Minelva R. Nanton", "Sean-Paul Nuccio", "Stephen J. McSorley", "Renée M. Tsolis"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0004027.g005", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_P_yoelii_infection_increases_PD1_expression_on_NTS_specific_CD4_T_cells_/1542202", "title"=>"<i>P</i>. <i>yoelii</i> infection increases PD1 expression on NTS-specific CD4 T cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-14 03:56:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/2262263"], "description"=>"<p>C57BL/6 mice were vaccinated with <i>S</i>. Typhimurium BRD509::2W1S (Vac) and then given <i>P</i>. <i>yoelii</i> (<i>P</i>.<i>y</i>.) as described above. (<b>A</b>) Expression analysis of inflammatory markers by qRT-PCR at 14 d post malaria and 3 days post SL1344 challenge (D17). Transcript levels of interferon-γ (<i>Ifng</i>) and interleukin-10 (<i>Il10</i>) were determined in whole spleen tissue, normalized to beta-actin levels and shown as fold-change over mock-treated mice. Bars indicate mean+SEM (n = 4). (<b>B-C</b>) IL-10 blockade was performed as described in methods. (<b>B</b>) Parasite burden, shown as % infected red blood cells (RBCs), was determined on Giemsa-stained blood smears from tails. (<b>C</b>). Antibody-treated mice were challenged with 750 CFU of virulent <i>S</i>. Typhimurium via the i.v. route at 14 days after <i>P</i>. <i>yoelii</i> infection. At 3 d post-challenge, bacterial burden was determined in livers and normalized to tissue weight. Data represent Mean+SEM (n = 4–5 per group). Significance of differences between groups was determined using a Student’s <i>t</i> test (*, <i>p</i><0.05; ns, not significant).</p>", "links"=>[], "tags"=>["Transient Loss", "effector capability", "CTLA", "NTS bloodstream", "CD 8 T cells", "memory CD 4 T cells", "Plasmodium falciparum malaria", "lag", "ifn", "NTS bloodstream infections", "protection", "molecule PD 1", "malaria parasite Plasmodium yoelii 17 XNL", "CD 4 T cell effector function", "immunocompetent individuals", "attenuated Salmonella vaccine", "African children", "malaria parasite infection", "PDL 1", "Simultaneous blockade", "Protection Afforded", "risk factor", "il"], "article_id"=>1542201, "categories"=>["Uncategorised"], "users"=>["Jason P. Mooney", "Seung-Joo Lee", "Kristen L. Lokken", "Minelva R. Nanton", "Sean-Paul Nuccio", "Stephen J. McSorley", "Renée M. Tsolis"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0004027.g004", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_P_yoelii_infection_increases_IL_10_expression_and_IL_10_blockade_partially_restores_protection_/1542201", "title"=>"<i>P</i>. <i>yoelii</i> infection increases IL-10 expression and IL-10 blockade partially restores protection.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-14 03:56:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/2262261"], "description"=>"<p>C57BL/6 mice were vaccinated with <i>S</i>. Typhimurium BRD509 (Vac). On day 42, these mice were inoculated with <i>P</i>. <i>yoelii</i> as described above. (<b>A</b>) At either 14 d (top) or 28 d post-malaria parasite infection (bottom), CD4 T cells were enriched from spleens of <i>P</i>. <i>yoelii</i>-infected or control mice. CD4 T cells were restimulated with Flagellin, SseI, or SseJ peptides (<a href=\"http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0004027#pntd.0004027.t001\" target=\"_blank\">Table 1</a>) in the presence of irradiated naïve splenocytes, and IFN-γ production was examined. Anti-CD3/anti-CD28 antibodies and no peptide (medium) wells constituted the positive and negative controls, respectively. Bar graphs show the number of IFN-γ-producing CD4 T cells per 2x10<sup>5</sup> CD4 T cells. (<b>B</b>) CD8 T cells were enriched from the spleens of the same mice, and stimulated with peptides corresponding to Class I epitopes from SseB, SseI and SseJ (<a href=\"http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0004027#pntd.0004027.t001\" target=\"_blank\">Table 1</a>). Data represent Mean+SEM of three independent experiments (n = 9). Significance of differences between groups was determined using a Student’s <i>t</i> test (*, <i>p</i><0.05; ns, not significant).</p>", "links"=>[], "tags"=>["Transient Loss", "effector capability", "CTLA", "NTS bloodstream", "CD 8 T cells", "memory CD 4 T cells", "Plasmodium falciparum malaria", "lag", "ifn", "NTS bloodstream infections", "protection", "molecule PD 1", "malaria parasite Plasmodium yoelii 17 XNL", "CD 4 T cell effector function", "immunocompetent individuals", "attenuated Salmonella vaccine", "African children", "malaria parasite infection", "PDL 1", "Simultaneous blockade", "Protection Afforded", "risk factor", "il"], "article_id"=>1542199, "categories"=>["Uncategorised"], "users"=>["Jason P. Mooney", "Seung-Joo Lee", "Kristen L. Lokken", "Minelva R. Nanton", "Sean-Paul Nuccio", "Stephen J. McSorley", "Renée M. Tsolis"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0004027.g002", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_IFN_producing_capability_of_Salmonella_specific_CD4_and_CD8_T_cells_is_reduced_during_peak_malaria_parasite_infection_upon_ex_vivo_stimulation_/1542199", "title"=>"IFN-γ-producing capability of <i>Salmonella</i>-specific CD4 and CD8 T cells is reduced during peak malaria-parasite infection upon <i>ex vivo</i> stimulation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-14 03:56:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/2262262"], "description"=>"<p>Mice were vaccinated with <i>S</i>. Typhimurium BRD509::2W1S (Vac). On day 42, vaccinated mice were infected with <i>P</i>. <i>yoelii</i> (<i>P</i>.<i>y</i>.). At 14 days post-malaria parasite infection, cells were analyzed. (<b>A</b>) Spleens and livers were analyzed by flow cytometry. Using 2W1S::I-A<sup>b</sup> tetramer staining on CD4 T cells, we evaluated both the percentage (%) and absolute number (#) of 2W1S-specific CD4 T cell populations in the liver and spleen. Data are shown as Mean+SEM (n = 3–4). Representative dot plots are shown in <a href=\"http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0004027#pntd.0004027.s005\" target=\"_blank\">S5 Fig</a>. To simulate CD4 T cells <i>in vivo</i>, <i>P</i>. <i>yoelii</i>-infected or control mice were treated i.v. with heat-killed <i>S</i>. Typhimurium lysates (+ HKST) or PBS. After 4 hours, splenic CD44+ CD4 T cells (<b>B</b>) and 2W1S-specific CD4 T cells (<b>C</b>) were analyzed by flow cytometry for IFN-γ production. Data represent Mean+SEM of three independent experiments (n = 9). To assess antigen-independent stimulation, splenic CD4 T cells were stimulated with PMA and Ionomycin (Iono) for 4 hours. IFN-γ production was evaluated by flow cytometry for antigen-experienced (CD44+) <b>(D)</b> or all CD4 T cells <b>(E)</b>. Bars represent Mean+SEM (n = 3–4). Significance of differences between groups was determined using a Student’s <i>t</i> test (*, <i>p</i><0.05; ns, not significant).</p>", "links"=>[], "tags"=>["Transient Loss", "effector capability", "CTLA", "NTS bloodstream", "CD 8 T cells", "memory CD 4 T cells", "Plasmodium falciparum malaria", "lag", "ifn", "NTS bloodstream infections", "protection", "molecule PD 1", "malaria parasite Plasmodium yoelii 17 XNL", "CD 4 T cell effector function", "immunocompetent individuals", "attenuated Salmonella vaccine", "African children", "malaria parasite infection", "PDL 1", "Simultaneous blockade", "Protection Afforded", "risk factor", "il"], "article_id"=>1542200, "categories"=>["Uncategorised"], "users"=>["Jason P. Mooney", "Seung-Joo Lee", "Kristen L. Lokken", "Minelva R. Nanton", "Sean-Paul Nuccio", "Stephen J. McSorley", "Renée M. Tsolis"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0004027.g003", "stats"=>{"downloads"=>1, "page_views"=>25, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_IFN_producing_capability_of_Salmonella_specific_CD4_T_cells_is_reduced_during_peak_malaria_parasite_infection_/1542200", "title"=>"IFN-γ-producing capability of <i>Salmonella</i>-specific CD4 T cells is reduced during peak malaria-parasite infection.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-14 03:56:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/2262260"], "description"=>"<p>C57BL/6 mice were vaccinated i.v. with 5x10<sup>5</sup> CFU attenuated <i>S</i>. Typhimurium BRD509 (Vac). Forty-two days later, a group of vaccinated mice was inoculated i.p. with blood containing 4x10<sup>7</sup><i>P</i>. <i>yoelii</i>-infected red blood cells (RBC) and the rest were given control blood. (<b>A</b>) Parasite burden, shown as % infected red blood cells (RBCs) (n = 25). Data represent Mean±SEM. (<b>B</b>) RBC concentrations (10<sup>6</sup>/uL) at either 14 or 28 days after malaria parasite inoculation as determined by complete blood counts (n = 4–8). Bars represent the Mean+SEM. (<b>C</b>) Mice were challenged with 750–1000 CFU of virulent <i>S</i>. Typhimurium via the i.v. route at either 14 days (<b>C</b>, left) or 28 days (<b>C</b>, right) after <i>P</i>. <i>yoelii</i> infection. At 3 d post-challenge, bacterial burden was determined in livers from mice infected with <i>S</i>. Typhimurium only (Control) or co-infected (<i>P</i>. <i>yoelii</i>). (<b>D</b>) C57BL/6 (n = 5), which are <i>Nramp1</i>/<i>Slc11a1</i><sup><i>-/-</i></sup>, or C57BL/6 expressing a functional allele of <i>Slc11a1</i> (n = 3–13), were vaccinated, then inoculated with <i>P</i>. <i>yoelii</i> and challenged i.v. with virulent <i>S</i>. Typhimurium at 14 days after <i>P</i>. <i>yoelii</i> infection. <i>S</i>. Typhimurium burden was quantified in the liver at 3 days after challenge with virulent <i>S</i>. Typhimurium. X, not performed. Data represent Mean+SEM (n = 4–8). Significance of differences between groups was determined using a Student’s <i>t</i> test (*, <i>p</i><0.05; ns, not significant).</p>", "links"=>[], "tags"=>["Transient Loss", "effector capability", "CTLA", "NTS bloodstream", "CD 8 T cells", "memory CD 4 T cells", "Plasmodium falciparum malaria", "lag", "ifn", "NTS bloodstream infections", "protection", "molecule PD 1", "malaria parasite Plasmodium yoelii 17 XNL", "CD 4 T cell effector function", "immunocompetent individuals", "attenuated Salmonella vaccine", "African children", "malaria parasite infection", "PDL 1", "Simultaneous blockade", "Protection Afforded", "risk factor", "il"], "article_id"=>1542198, "categories"=>["Uncategorised"], "users"=>["Jason P. Mooney", "Seung-Joo Lee", "Kristen L. Lokken", "Minelva R. Nanton", "Sean-Paul Nuccio", "Stephen J. McSorley", "Renée M. Tsolis"], "doi"=>"https://dx.doi.org/10.1371/journal.pntd.0004027.g001", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transient_loss_in_protection_to_Salmonella_challenge_during_peak_malaria_parasite_infection_in_Salmonella_vaccinated_mice_/1542198", "title"=>"Transient loss in protection to <i>Salmonella</i> challenge during peak malaria-parasite infection in <i>Salmonella</i>-vaccinated mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-14 03:56:51"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"42", "full-text"=>"41", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"30", "full-text"=>"25", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"17", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"17", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"14", "full-text"=>"17", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"11", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"2", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"17", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"9", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2020", "month"=>"10"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts