The microbiome composition of Aedes aegypti is not critical for Wolbachia-mediated inhibition of dengue virus
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"The microbiome composition of Aedes aegypti is not critical for Wolbachia-mediated inhibition of dengue virus", "type"=>"journal", "authors"=>[{"first_name"=>"Michelle D.", "last_name"=>"Audsley", "scopus_author_id"=>"36146679900"}, {"first_name"=>"Yixin H.", "last_name"=>"Ye", "scopus_author_id"=>"26640777600"}, {"first_name"=>"Elizabeth A.", "last_name"=>"McGraw", "scopus_author_id"=>"6603395546"}], "year"=>2017, "source"=>"PLoS Neglected Tropical Diseases", "identifiers"=>{"sgr"=>"85016948649", "pmid"=>"28267749", "pui"=>"615136291", "issn"=>"19352735", "isbn"=>"1111111111", "scopus"=>"2-s2.0-85016948649", "doi"=>"10.1371/journal.pntd.0005426"}, "id"=>"1b00399b-7a03-378a-9622-43e7f99d0724", "abstract"=>"BACKGROUND Dengue virus (DENV) is primarily vectored by the mosquito Aedes aegypti, and is estimated to cause 390 million human infections annually. A novel method for DENV control involves stable transinfection of Ae. aegypti with the common insect endosymbiont Wolbachia, which mediates an antiviral effect. However, the mechanism by which Wolbachia reduces the susceptibility of Ae. aegypti to DENV is not fully understood. In this study we assessed the potential of resident microbiota, which can play important roles in insect physiology and immune responses, to affect Wolbachia-mediated DENV blocking. METHODOLOGY/FINDINGS The microbiome of Ae. aegypti stably infected with Wolbachia strain wMel was compared to that of Ae. aegypti without Wolbachia, using 16s rDNA profiling. Our results indicate that although Wolbachia affected the relative abundance of several genera, the microbiome of both the Wolbachia-infected and uninfected mosquitoes was dominated by Elizabethkingia and unclassified Enterobacteriaceae. To assess the potential of the resident microbiota to affect the Wolbachia-mediated antiviral effect, we used antibiotic treatment before infection with DENV by blood-meal. In spite of a significant shift in the microbiome composition in response to the antibiotics, we detected no effect of antibiotic treatment on DENV infection rates, or on the DENV load of infected mosquitoes. CONCLUSIONS/SIGNIFICANCE Our findings indicate that stable infection with Wolbachia strain wMel produces few effects on the microbiome of laboratory-reared Ae. aegypti. Moreover, our findings suggest that the microbiome can be significantly altered without affecting the fundamental DENV blocking phenotype in these mosquitoes. Since Ae. aegypti are likely to encounter diverse microbiota in the field, this is a particularly important result in the context of using Wolbachia as a method for DENV control.", "link"=>"http://www.mendeley.com/research/microbiome-composition-aedes-aegypti-not-critical-wolbachiamediated-inhibition-dengue-virus", "reader_count"=>62, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>2, "Librarian"=>1, "Researcher"=>17, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>13, "Student > Postgraduate"=>2, "Student > Master"=>6, "Other"=>4, "Student > Bachelor"=>10, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>2, "Librarian"=>1, "Researcher"=>17, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>13, "Student > Postgraduate"=>2, "Student > Master"=>6, "Other"=>4, "Student > Bachelor"=>10, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Engineering"=>2, "Environmental Science"=>4, "Biochemistry, Genetics and Molecular Biology"=>8, "Agricultural and Biological Sciences"=>35, "Medicine and Dentistry"=>3, "Design"=>1, "Veterinary Science and Veterinary Medicine"=>3, "Chemistry"=>1, "Computer Science"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Design"=>{"Design"=>1}, "Engineering"=>{"Engineering"=>2}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Chemistry"=>{"Chemistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>35}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>8}, "Unspecified"=>{"Unspecified"=>3}, "Environmental Science"=>{"Environmental Science"=>4}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>3}}, "reader_count_by_country"=>{"United States"=>4, "Brazil"=>1, "Mexico"=>1, "United Kingdom"=>1, "France"=>1}, "group_count"=>4}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/85016948649"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/85016948649?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85016948649&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85016948649&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/85016948649", "dc:identifier"=>"SCOPUS_ID:85016948649", "eid"=>"2-s2.0-85016948649", "dc:title"=>"The microbiome composition of Aedes aegypti is not critical for Wolbachia-mediated inhibition of dengue virus", "dc:creator"=>"Audsley M.", "prism:publicationName"=>"PLoS Neglected Tropical Diseases", "prism:issn"=>"19352727", "prism:eIssn"=>"19352735", "prism:volume"=>"11", "prism:issueIdentifier"=>"3", "prism:pageRange"=>nil, "prism:coverDate"=>"2017-03-07", "prism:coverDisplayDate"=>"7 March 2017", "prism:doi"=>"10.1371/journal.pntd.0005426", "citedby-count"=>"11", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Monash University", "affiliation-city"=>"Melbourne", "affiliation-country"=>"Australia"}], "pubmed-id"=>"28267749", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0005426", "source-id"=>"11900154404", "openaccess"=>"1", "openaccessFlag"=>true}

Twitter

Counter

  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"157", "xml_views"=>"4", "html_views"=>"1350"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"59", "xml_views"=>"1", "html_views"=>"182"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"44", "xml_views"=>"2", "html_views"=>"146"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"117"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"105"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"47", "xml_views"=>"0", "html_views"=>"118"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"24", "xml_views"=>"2", "html_views"=>"110"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"90"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"84"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"59"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"50", "xml_views"=>"0", "html_views"=>"86"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"29", "xml_views"=>"1", "html_views"=>"47"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"25", "xml_views"=>"1", "html_views"=>"60"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"19", "xml_views"=>"3", "html_views"=>"32"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"3", "html_views"=>"32"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"16"}

PMC Usage Stats

  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"33", "full-text"=>"42", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"24", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"19", "full-text"=>"18", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"24", "full-text"=>"26", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"21", "full-text"=>"22", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"22", "full-text"=>"20", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"21", "full-text"=>"21", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"25", "full-text"=>"26", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"21", "full-text"=>"28", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"18", "full-text"=>"23", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"17", "full-text"=>"14", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"26", "full-text"=>"25", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"9", "pdf"=>"10", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"23", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"16", "full-text"=>"19", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"22", "full-text"=>"24", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"18", "full-text"=>"57", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
Loading … Spinner
There are currently no alerts