Zika virus-like particle (VLP) based vaccine
Publication Date
May 08, 2017
Authors
Hélène Boigard, Alexandra Alimova, George R. Martin, Al Katz, et al
Volume
11
Issue
5
Pages
e0005608
DOI
https://dx.plos.org/10.1371/journal.pntd.0005608
Publisher URL
http://journals.plos.org/plosntds/article?id=10.1371%2Fjournal.pntd.0005608
Scopus
85020453384
Mendeley
http://www.mendeley.com/research/zika-viruslike-particle-vlp-based-vaccine
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{"title"=>"Zika virus-like particle (VLP) based vaccine", "type"=>"journal", "authors"=>[{"first_name"=>"Hélène", "last_name"=>"Boigard", "scopus_author_id"=>"57189616525"}, {"first_name"=>"Alexandra", "last_name"=>"Alimova", "scopus_author_id"=>"6602472834"}, {"first_name"=>"George R.", "last_name"=>"Martin", "scopus_author_id"=>"57194495066"}, {"first_name"=>"Al", "last_name"=>"Katz", "scopus_author_id"=>"7402569330"}, {"first_name"=>"Paul", "last_name"=>"Gottlieb", "scopus_author_id"=>"55729101800"}, {"first_name"=>"Jose M.", "last_name"=>"Galarza", "scopus_author_id"=>"6602531823"}], "year"=>2017, "source"=>"PLoS Neglected Tropical Diseases", "identifiers"=>{"pui"=>"616682010", "isbn"=>"1111111111", "issn"=>"19352735", "doi"=>"10.1371/journal.pntd.0005608", "scopus"=>"2-s2.0-85020453384", "pmid"=>"28481898", "sgr"=>"85020453384"}, "id"=>"3379e029-7122-3fd9-853c-d66b45881dd9", "abstract"=>"The newly emerged mosquito-borne Zika virus poses a major public challenge due to its ability to cause significant birth defects and neurological disorders. The impact of sexual transmission is unclear but raises further concerns about virus dissemination. No specific treatment or vaccine is currently available, thus the development of a safe and effective vaccine is paramount. Here we describe a novel strategy to assemble Zika virus-like particles (VLPs) by co-expressing the structural (CprME) and non-structural (NS2B/NS3) proteins, and demonstrate their effectiveness as vaccines. VLPs are produced in a suspension culture of mammalian cells and self-assembled into particles closely resembling Zika viruses as shown by electron microscopy studies. We tested various VLP vaccines and compared them to analogous compositions of an inactivated Zika virus (In-ZIKV) used as a reference. VLP immunizations elicited high titers of antibodies, as did the In-ZIKV controls. However, in mice the VLP vaccine stimulated significantly higher virus neutralizing antibody titers than comparable formulations of the In-ZIKV vaccine. The serum neutralizing activity elicited by the VLP vaccine was enhanced using a higher VLP dose and with the addition of an adjuvant, reaching neutralizing titers greater than those detected in the serum of a patient who recovered from a Zika infection in Brazil in 2015. Discrepancies in neutralization levels between the VLP vaccine and the In-ZIKV suggest that chemical inactivation has deleterious effects on neutralizing epitopes within the E protein. This along with the inability of a VLP vaccine to cause infection makes it a preferable candidate for vaccine development.", "link"=>"http://www.mendeley.com/research/zika-viruslike-particle-vlp-based-vaccine", "reader_count"=>66, "reader_count_by_academic_status"=>{"Unspecified"=>6, "Professor > Associate Professor"=>1, "Researcher"=>12, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>12, "Student > Postgraduate"=>4, "Other"=>5, "Student > Master"=>10, "Student > Bachelor"=>12, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>6, "Professor > Associate Professor"=>1, "Researcher"=>12, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>12, "Student > Postgraduate"=>4, "Other"=>5, "Student > Master"=>10, "Student > Bachelor"=>12, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>12, "Agricultural and Biological Sciences"=>9, "Veterinary Science and Veterinary Medicine"=>1, "Chemical Engineering"=>1, "Chemistry"=>2, "Decision Sciences"=>1, "Engineering"=>2, "Biochemistry, Genetics and Molecular Biology"=>14, "Mathematics"=>1, "Medicine and Dentistry"=>8, "Pharmacology, Toxicology and Pharmaceutical Science"=>3, "Social Sciences"=>1, "Immunology and Microbiology"=>11}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>8}, "Social Sciences"=>{"Social Sciences"=>1}, "Decision Sciences"=>{"Decision Sciences"=>1}, "Mathematics"=>{"Mathematics"=>1}, "Unspecified"=>{"Unspecified"=>12}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>3}, "Chemical Engineering"=>{"Chemical Engineering"=>1}, "Engineering"=>{"Engineering"=>2}, "Chemistry"=>{"Chemistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>11}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>9}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>14}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "group_count"=>1}

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Scopus | Further Information

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