Acetylation Regulates WRN Catalytic Activities and Affects Base Excision DNA Repair
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Acetylation Regulates WRN Catalytic Activities and Affects Base Excision DNA Repair", "type"=>"journal", "authors"=>[{"first_name"=>"Meltem", "last_name"=>"Muftuoglu"}, {"first_name"=>"Rika", "last_name"=>"Kusumoto"}, {"first_name"=>"Elzbieta", "last_name"=>"Speina"}, {"first_name"=>"Gad", "last_name"=>"Beck"}, {"first_name"=>"Wen-Hsing", "last_name"=>"Cheng"}, {"first_name"=>"Vilhelm A.", "last_name"=>"Bohr"}], "year"=>2008, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"18398454", "doi"=>"10.1371/journal.pone.0001918", "isbn"=>"1932-6203 (Electronic)", "issn"=>"1932-6203"}, "id"=>"05d59ebb-f485-31b9-86c5-cd603dd62ad9", "abstract"=>"BACKGROUND: The Werner protein (WRN), defective in the premature aging disorder Werner syndrome, participates in a number of DNA metabolic processes, and we have been interested in the possible regulation of its function in DNA repair by post-translational modifications. Acetylation mediated by histone acetyltransferases is of key interest because of its potential importance in aging, DNA repair and transcription. METHODOLOGY/PRINCIPAL FINDINGS: Here, we have investigated the p300 acetylation mediated changes on the function of WRN in base excision DNA repair (BER). We show that acetylation of WRN increases in cells treated with methyl methanesulfonate (MMS), suggesting that acetylation of WRN may play a role in response to DNA damage. This hypothesis is consistent with our findings that acetylation of WRN stimulates its catalytic activities in vitro and in vivo, and that acetylated WRN enhances pol beta-mediated strand displacement DNA synthesis more than unacetylated WRN. Furthermore, we show that cellular exposure to the histone deacetylase inhibitor sodium butyrate stimulates long patch BER in wild type cells but not in WRN depleted cells, suggesting that acetylated WRN participates significantly in this process. CONCLUSION/SIGNIFICANCE: Collectively, these results provide the first evidence for a specific role of p300 mediated WRN acetylation in regulating its function during BER.", "link"=>"http://www.mendeley.com/research/acetylation-regulates-wrn-catalytic-activities-affects-base-excision-dna-repair", "reader_count"=>24, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>7, "Student > Ph. D. Student"=>8, "Student > Postgraduate"=>2, "Student > Master"=>1, "Student > Bachelor"=>1, "Lecturer"=>2, "Professor"=>2}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>7, "Student > Ph. D. Student"=>8, "Student > Postgraduate"=>2, "Student > Master"=>1, "Student > Bachelor"=>1, "Lecturer"=>2, "Professor"=>2}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>4, "Agricultural and Biological Sciences"=>17, "Medicine and Dentistry"=>2, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>17}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}}, "reader_count_by_country"=>{"Vietnam"=>1, "China"=>1, "United Kingdom"=>2, "Germany"=>2}, "group_count"=>1}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/44849140553"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/44849140553?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=44849140553&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=44849140553&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/44849140553", "dc:identifier"=>"SCOPUS_ID:44849140553", "eid"=>"2-s2.0-44849140553", "dc:title"=>"Acetylation regulation WRN catalytic activities and affects base excision DNA repair", "dc:creator"=>"Muftuoglu M.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"3", "prism:issueIdentifier"=>"4", "prism:pageRange"=>nil, "prism:coverDate"=>"2008-04-09", "prism:coverDisplayDate"=>"9 April 2008", "prism:doi"=>"10.1371/journal.pone.0001918", "citedby-count"=>"26", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"National Institute on Aging (NIA)", "affiliation-city"=>"Bethesda", "affiliation-country"=>"United States"}], "pubmed-id"=>"18398454", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e1918", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0001918", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Journal Comments | Further Information

Counter

  • {"month"=>"4", "year"=>"2008", "pdf_views"=>"86", "xml_views"=>"40", "html_views"=>"285"}
  • {"month"=>"5", "year"=>"2008", "pdf_views"=>"33", "xml_views"=>"45", "html_views"=>"87"}
  • {"month"=>"6", "year"=>"2008", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"7", "year"=>"2008", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"8", "year"=>"2008", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"9", "year"=>"2008", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"45"}
  • {"month"=>"10", "year"=>"2008", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"11", "year"=>"2008", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"12", "year"=>"2008", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"1", "year"=>"2009", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"2", "year"=>"2009", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"3", "year"=>"2009", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"4", "year"=>"2009", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2009", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"6", "year"=>"2009", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"7", "year"=>"2009", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"8", "year"=>"2009", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"9", "year"=>"2009", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"10", "year"=>"2009", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"11", "year"=>"2009", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"12", "year"=>"2009", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"1", "year"=>"2010", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"2", "year"=>"2010", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"3", "year"=>"2010", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"4", "year"=>"2010", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"5", "year"=>"2010", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"6", "year"=>"2010", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"7", "year"=>"2010", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"8", "year"=>"2010", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"9", "year"=>"2010", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"40"}
  • {"month"=>"10", "year"=>"2010", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"38"}
  • {"month"=>"11", "year"=>"2010", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"12", "year"=>"2010", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"1", "year"=>"2011", "pdf_views"=>"4", "xml_views"=>"3", "html_views"=>"14"}
  • {"month"=>"2", "year"=>"2011", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"3", "year"=>"2011", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"4", "year"=>"2011", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"5", "year"=>"2011", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"6", "year"=>"2011", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"7", "year"=>"2011", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"8", "year"=>"2011", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"9", "year"=>"2011", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"10", "year"=>"2011", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"11", "year"=>"2011", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"5", "html_views"=>"14"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"0", "xml_views"=>"2", "html_views"=>"17"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"35"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"38"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"21"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"0", "xml_views"=>"2", "html_views"=>"20"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"71"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"74"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"68"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"12"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"11"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"3", "html_views"=>"10"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"3"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"4"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"3"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/934584"], "description"=>"<p>Comparison of uracil-DNA mediated BER in extracts from wild-type (WT) and WRN KD cells treated with or without 5 mM sodium butyrate. (A) Substrate DNA and predicted BER reaction products and intermediates. The sizes of intermediates (LP BER, 2 nt addition; SN or LP BER, 1 nt addition) and complete BER (ligated 34mer SN BER) product are shown. (B) Phosphorimages of denaturing polyacrylamide gels showing <i>in vitro</i> BER products. The reaction conditions and products analyses are described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001918#s4\" target=\"_blank\">Materials and Methods</a>. A 34 bp duplex substrate containing a uracil residue at position 16 was incubated with [<sup>32</sup>P]dCTP and ddGTP and WT or WRN KD cell extracts (with or without treatment with NaB), as indicated. The positions of the BER products are indicated. (C) Quantitation of LP BER products from three different experiments is plotted. (D) Top panel: After transfection, the cells were treated with 5 mM sodium butyrate for 24 h and labeled with 0.5 mCi/ml [<sup>3</sup>H]sodium acetate for 1 h. [<sup>3</sup>H]acetate labeled WRN was immunoprecipitated using an anti-WRN antibody and the complex was resolved on SDS-PAGE and analyzed by autoradiography. Lower panel: Western analysis of acetylated samples with anti-WRN antibody.</p>", "links"=>[], "tags"=>["butyrate", "lp"], "article_id"=>605025, "categories"=>["Biochemistry", "Molecular Biology", "Genetics"], "users"=>["Meltem Muftuoglu", "Rika Kusumoto", "Elzbieta Speina", "Gad Beck", "Wen-Hsing Cheng", "Vilhelm A. Bohr"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0001918.g009", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sodium_butyrate_treatment_enhances_LP_BER_/605025", "title"=>"Sodium butyrate treatment enhances LP BER.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2008-04-09 01:23:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/934260"], "description"=>"<p>(A) A schematic representation of GST- WRN<sub>488–1432</sub> fragment. (B) Recombinant WRN<sub>239–499</sub> and WRN<sub>488–1432</sub> fragments were separated by SDS-PAGE, transferred to a membrane, and stained with amido black to ensure equal loading of the WRN fragments (bottom panel, lanes 1 and 2). The membrane was blotted with p300 protein and probed with mouse anti-p300 antibody (top panel, lanes 1 and 2). (C) One microgram of WRN<sub>488–1432</sub> fragment was incubated with in the presence or absence of acetyl CoA or p300 or both. The top panel shows Western analysis results of WRN<sub>488–1432</sub> fragment after the acetylation assay, which was stripped and analyzed using an anti-acetyl lysine antibody (bottom panel). These reaction mixtures were used in the measurement of helicase activity (medium panel). Medium panel: Reactions containing increasing amounts (7.5, 15, and 30 fmol) of nonacetylated WRN<sub>488–1432</sub> fragment (lanes 2 to 4) and acetylated WRN<sub>488–1432</sub> fragment (lanes 8 to 10) were incubated with a 22 bp forked substrate (0.5 nM, 22Fork3/22Fork4) for 15 min at 37°C. Lanes 5 to 7, WRN<sub>488–1432</sub> fragment in the presence of p300. Lane 1, substrate only. Lane 11, heat-denatured substrate. Reaction products were run on a 12% native gel and visualized using a PhosphorImager. Bottom panel: Quantitation of percentage of unwinding product.</p>", "links"=>[], "tags"=>["stimulates", "helicase"], "article_id"=>604706, "categories"=>["Biochemistry", "Molecular Biology", "Genetics"], "users"=>["Meltem Muftuoglu", "Rika Kusumoto", "Elzbieta Speina", "Gad Beck", "Wen-Hsing Cheng", "Vilhelm A. Bohr"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0001918.g006", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Acetylation_of_WRN_488_8211_1432_stimulates_its_helicase_activity_/604706", "title"=>"Acetylation of WRN<sub>488–1432</sub> stimulates its helicase activity.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2008-04-09 01:18:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/934135"], "description"=>"<p>(A) Co-immunoprecipitation of WRN and p300. HeLa nuclear extracts were immunoprecipitated with either polyclonal rabbit anti-WRN antibodies (lane 3) or control IgG (lane 2). The immunoprecipitates were analyzed by SDS-PAGE and Western analysis with anti-p300 or anti-WRN antibodies, as indicated. Input, 10% loaded (lane 1). (B) HeLa nuclear extracts were immunoprecipitated with either polyclonal rabbit anti-p300 antibodies (lane 3) or control IgG (lane 1). The immunoprecipitates were probed with anti-WRN or anti-p300 antibodies, as indicated. Input, 10% loaded (lane 2). (C) WRN and p300 were co-immunoprecipitated from HeLa nuclear extracts either in the absence (lanes 4 and 5) or the presence (lanes 1 to 3) of ethidium bromide (Et.Br.; 50 µg/ml) with anti-WRN antibodies (lanes 2 and 5) but not with the control antibodies (lane 1), as indicated by Western analysis with anti-p300 antibodies (upper) and anti-WRN antibodies (lower). Inputs are 10% (lanes 2 and 5). (D) HeLa nuclear extracts (400 µL) were incubated with either GST alone (lane 2) or GST-tagged WRN fragments (lanes 3–6) that were prebound to glutathione beads. Eluted proteins were separated by SDS-PAGE, transferred to a membrane, and stained with amido black to ensure equal loading of the various WRN fragments. The membrane was probed with mouse anti-p300 antibody. The input (lane 1) corresponds to 5% of HeLa nuclear extracts used in the binding reactions. The <i>asterisk</i> indicates mobility of full-length GST-WRN helicase domain (aa 500–946).</p>", "links"=>[], "tags"=>["biochemistry/replication and repair", "genetics and genomics/disease models", "molecular biology/dna repair"], "article_id"=>604581, "categories"=>["Biochemistry", "Molecular Biology", "Genetics"], "users"=>["Meltem Muftuoglu", "Rika Kusumoto", "Elzbieta Speina", "Gad Beck", "Wen-Hsing Cheng", "Vilhelm A. Bohr"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0001918.g005", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Characterization_of_the_WRN_p300_physical_interaction_/604581", "title"=>"Characterization of the WRN/p300 physical interaction.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2008-04-09 01:16:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/933757"], "description"=>"<p>(A) WRN was transiently overexpressed in 293T cells either alone or together with p300. 48 h after transfection, untreated, UV, H<sub>2</sub>O<sub>2</sub> or MMS treated cells were labeled with 1 mCi/ml [<sup>3</sup>H]sodium acetate for 1 h. [<sup>3</sup>H]acetate labeled WRN was immunoprecipitated using an anti-WRN antibody and the complex was resolved on SDS-PAGE and was analyzed either by autoradiography (upper panel) or Western analysis (lower panel) or coomassie-stained gel after the acetylation assay, which was subsequently, analyzed using autoradiography (middle panel). (B) Right and left top panels: 48 h after transfection, the cells were treated with 1 mM MMS and WRN acetylation was followed for 1 h and 4 h (left panel) or 1 h (right panel), and labeled with 0.5 mCi/ml [<sup>3</sup>H]sodium acetate for 1 h. [<sup>3</sup>H]acetate labeled WRN was immunoprecipitated using an anti-WRN antibody and the complex was resolved on SDS-PAGE and analyzed by autoradiography. Lower panels: Western analysis of acetylated samples with an anti-WRN antibody. (C) Top panel: 48 h after transfection, the cells were treated with H<sub>2</sub>O<sub>2</sub> (1 and 4 h), γ-irradiation (1 and 4 h) or psoralen+UVA (1 and 16 h) and labeled with 0.5 mCi/ml [<sup>3</sup>H]sodium acetate for 1 h. [<sup>3</sup>H]acetate labeled WRN was immunoprecipitated using an anti-WRN antibody and the complex was resolved on SDS-PAGE and analyzed by autoradiography. Medium panel: the coomassie staining of the gel before autoradiography. Lower panel: Western analysis of acetylated samples with an anti-WRN antibody. (D) Top panel: After transfection, the cells were treated with 50 µM and 200 µM H<sub>2</sub>O<sub>2</sub> and labeled with 0.5 mCi/ml [<sup>3</sup>H]sodium acetate for 1 h. Lower panel: Western analysis of acetylated samples with an anti-WRN antibody. (E) 293T cells were transiently transfected with expression vectors of WRN (lanes 1–3), p300 (lane 3), siRNA of p300 (lane 2) or siRNA negative control (lanes 1 and 4) individually or in combination. [<sup>3</sup>H]acetate labeled WRN was immunoprecipitated using an anti-WRN antibody and the complex was resolved on SDS-PAGE and was analyzed either by autoradiography (first panel) or Western analysis (second panel). Two bottom panels are Western analysis of these cell lysates with anti-p300 and anti-β-Actin antibodies, which shows relative protein expression levels.</p>", "links"=>[], "tags"=>["acetylated", "stimulated", "mms"], "article_id"=>604200, "categories"=>["Biochemistry", "Molecular Biology", "Genetics"], "users"=>["Meltem Muftuoglu", "Rika Kusumoto", "Elzbieta Speina", "Gad Beck", "Wen-Hsing Cheng", "Vilhelm A. Bohr"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0001918.g001", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_WRN_is_acetylated_in_vivo_and_it_is_stimulated_by_MMS_treatment_/604200", "title"=>"WRN is acetylated <i>in vivo</i> and it is stimulated by MMS treatment.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2008-04-09 01:10:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/934046"], "description"=>"<p>(A) The top panel shows a WB of 293T cell extract transfected with or without p300 plasmid analyzed using an anti-acetyl lysine antibody; bottom panel shows the same membrane stripped and analyzed with an anti-WRN antibody. These cell extracts were used in the measurement of WRN exonuclease activities (B). (B) Lysates from 293T cells with (lane 3) or without (lanes 1 and 2) transfection with p300 expression plasmid were immunoprecipitated with antibodies against immunoglobulin G (IgG) (lane 1) or WRN (lanes 2 and 3). The washed immunocomplexes were incubated with the exonuclease substrate (top, 0.5 nM, final concentration) as described.</p>", "links"=>[], "tags"=>["wrn", "exonuclease"], "article_id"=>604490, "categories"=>["Biochemistry", "Molecular Biology", "Genetics"], "users"=>["Meltem Muftuoglu", "Rika Kusumoto", "Elzbieta Speina", "Gad Beck", "Wen-Hsing Cheng", "Vilhelm A. Bohr"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0001918.g004", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Acetylation_of_WRN_increases_its_exonuclease_activity_in_vivo_/604490", "title"=>"Acetylation of WRN increases its exonuclease activity <i>in vivo</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2008-04-09 01:14:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/934470"], "description"=>"<p>(A) Reactions contained Pol β alone (1 nM) or together with increasing concentrations of WRN (6 and 12 nM) or acetylated WRN or WRN plus p300 or p300 plus acetyl CoA. The reactions were initiated by adding 12.5 nM one nucleotide gap substrate and were incubated for 25 minutes at 37°C, followed by analysis on a 20% denaturing gel. (B) Quantitation of short (1 nt) and long-patch (2–6) BER intermediates.</p>", "links"=>[], "tags"=>["wrn", "stimulates", "strand", "displacement", "dna", "synthesis"], "article_id"=>604911, "categories"=>["Biochemistry", "Molecular Biology", "Genetics"], "users"=>["Meltem Muftuoglu", "Rika Kusumoto", "Elzbieta Speina", "Gad Beck", "Wen-Hsing Cheng", "Vilhelm A. Bohr"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0001918.g008", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Acetylation_of_WRN_stimulates_strand_displacement_DNA_synthesis_by_Pol_946_/604911", "title"=>"Acetylation of WRN stimulates strand displacement DNA synthesis by Polβ.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2008-04-09 01:21:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/933961"], "description"=>"<p>(A) One microgram of WRN was incubated with in the presence or absence of acetyl CoA or p300 or both. The top panel shows a WB of WRN after the acetylation assay, which was stripped and analyzed using anti-acetyl lysine antibody (bottom panel). These reaction mixtures were used in the measurement of WRN ATPase (B), helicase (C) and exonuclease activities (D). (B) Acetylation increases the ATPase activity of WRN. [<sup>32</sup>P]ATP and 150 ng of M13mp18 ssDNA was incubated with increasing concentrations of nonacetylated (open circles) and acetylated WRN (closed circles) (15, 45, 90, 135 nM). WRN was incubated with p300 (closed triangles) or with acetyl CoA (open triangles). ATP hydrolysis was analyzed on PEI-TLC plates developed in 1 M formic acid/0.8 M LiCl. Plates were scanned using a PhosphorImager. The percentage of ATP hydrolysis was determined by each sample using the ImageQuant software and subtracting background value in control having no enzyme. (C) The effect of acetylation on WRN helicase activity. Reactions containing increasing amounts (0.125 nM, 0.25 nM and 0.5 nM) of nonacetylated WRN (lanes 12 to 14) and acetylated WRN (lanes 3 to 5) were incubated with a 22 bp forked substrate (0.5 nM, 22Fork3/22Fork4) for 15 min at 37°C. Lanes 6 to 8, WRN in the presence of acetyl CoA. Lanes 9 to 11, WRN in the presence of p300. Lane 1, substrate only. Lane 2, heat-denatured substrate. Reaction products were run on a 12% native gel and visualized using a PhosphorImager. %D, percentage of single-stranded product displaced. (D) The effect of WRN acetylation on WRN exonuclease activity. Reactions contained 10 and 20 nM nonacetylated WRN (lanes 4–5) and acetylated WRN (lanes 2–3), and WRN plus p300 (lanes 6–7) were incubated with a 34 bp forked substrate (0.5 nM, 34ForkA/34ForkB) for 15 min at 37°C. Lane 1, no enzyme. Products were heat-denatured for 5 min at 95°C, run on a 14% denaturing polyacrylamide gel and visualized using a PhosphorImager.</p>", "links"=>[], "tags"=>["acetylation", "wrn", "catalytic"], "article_id"=>604406, "categories"=>["Biochemistry", "Molecular Biology", "Genetics"], "users"=>["Meltem Muftuoglu", "Rika Kusumoto", "Elzbieta Speina", "Gad Beck", "Wen-Hsing Cheng", "Vilhelm A. Bohr"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0001918.g003", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_acetylation_on_WRN_catalytic_activities_/604406", "title"=>"Effect of acetylation on WRN catalytic activities.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2008-04-09 01:13:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/933863"], "description"=>"<p>(A) Schematic representation of functional domains of WRN and GST-tagged WRN fragments. RQC, RecQ-conserved domain; NTS, nucleolar targeting sequence; HRDC, helicase and RNase D conserved domain; NLS, nuclear localization signal. Numbers indicate WRN amino acid sequence. (B) One microgram of WRN and WRN fragments were incubated with [<sup>14</sup>C]-acetyl-CoA in the presence or absence of p300. The lower panel shows a coomassie-stained SDS-PAGE gel of purified WRN and GST-WRN fragments after the acetylation assay, which was subsequently, analyzed using autoradiography (top panel). (C) One microgram of GST (control) and GST-WRN fragments were incubated with [<sup>14</sup>C]-acetyl-CoA in the presence or absence of p300. The lower panel shows a coomassie-stained SDS-PAGE gel purified GST-WRN fragments after the acetylation assay that was analyzed using autoradiography (top panel).</p>", "links"=>[], "tags"=>["wrn", "acetylation"], "article_id"=>604311, "categories"=>["Biochemistry", "Molecular Biology", "Genetics"], "users"=>["Meltem Muftuoglu", "Rika Kusumoto", "Elzbieta Speina", "Gad Beck", "Wen-Hsing Cheng", "Vilhelm A. Bohr"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0001918.g002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mapping_of_the_WRN_acetylation_site_/604311", "title"=>"Mapping of the WRN acetylation site.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2008-04-09 01:11:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/460083", "https://ndownloader.figshare.com/files/460091", "https://ndownloader.figshare.com/files/460101"], "description"=>"<div><h3>Background</h3><p>The Werner protein (WRN), defective in the premature aging disorder Werner syndrome, participates in a number of DNA metabolic processes, and we have been interested in the possible regulation of its function in DNA repair by post-translational modifications. Acetylation mediated by histone acetyltransferases is of key interest because of its potential importance in aging, DNA repair and transcription.</p><h3>Methodology/Principal Findings</h3><p>Here, we have investigated the p300 acetylation mediated changes on the function of WRN in base excision DNA repair (BER). We show that acetylation of WRN increases in cells treated with methyl methanesulfonate (MMS), suggesting that acetylation of WRN may play a role in response to DNA damage. This hypothesis is consistent with our findings that acetylation of WRN stimulates its catalytic activities <em>in vitro</em> and <em>in vivo</em>, and that acetylated WRN enhances pol β-mediated strand displacement DNA synthesis more than unacetylated WRN. Furthermore, we show that cellular exposure to the histone deacetylase inhibitor sodium butyrate stimulates long patch BER in wild type cells but not in WRN depleted cells, suggesting that acetylated WRN participates significantly in this process.</p><h3>Conclusion/Significance</h3><p>Collectively, these results provide the first evidence for a specific role of p300 mediated WRN acetylation in regulating its function during BER.</p></div>", "links"=>[], "tags"=>["acetylation", "regulates", "wrn", "catalytic", "activities", "affects", "excision", "dna"], "article_id"=>150648, "categories"=>["Biochemistry", "Molecular Biology", "Genetics"], "users"=>["Meltem Muftuoglu", "Rika Kusumoto", "Elzbieta Speina", "Gad Beck", "Wen-Hsing Cheng", "Vilhelm A. Bohr"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0001918.s001", "https://dx.doi.org/10.1371/journal.pone.0001918.s002", "https://dx.doi.org/10.1371/journal.pone.0001918.s003"], "stats"=>{"downloads"=>2, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Acetylation_Regulates_WRN_Catalytic_Activities_and_Affects_Base_Excision_DNA_Repair/150648", "title"=>"Acetylation Regulates WRN Catalytic Activities and Affects Base Excision DNA Repair", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2008-04-09 00:10:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/934354"], "description"=>"<p>Reactions were run under the conditions described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001918#s4\" target=\"_blank\">Materials and Methods</a> on a 5% acrylamide gel and visualized using a PhosphorImager. (A) Acetylated WRN and WRN-p300 complex have higher DNA binding affinity. (B) Acetylated WRN<sub>488–1432</sub> has higher DNA binding activity.</p>", "links"=>[], "tags"=>["wrn", "stimulates", "dna", "binding"], "article_id"=>604794, "categories"=>["Biochemistry", "Molecular Biology", "Genetics"], "users"=>["Meltem Muftuoglu", "Rika Kusumoto", "Elzbieta Speina", "Gad Beck", "Wen-Hsing Cheng", "Vilhelm A. Bohr"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0001918.g007", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Acetylation_of_WRN_and_WRN_488_8211_1432_stimulates_their_DNA_binding_activities_/604794", "title"=>"Acetylation of WRN and WRN<sub>488–1432</sub> stimulates their DNA binding activities.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2008-04-09 01:19:54"}

PMC Usage Stats | Further Information

  • {"month"=>"1", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"6", "year"=>"2010", "pdf"=>"3", "unique-ip"=>"8", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"2", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"5", "unique-ip"=>"7", "pdf"=>"0", "year"=>"2010", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"3", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"19", "year"=>"2010", "pdf"=>"9", "unique-ip"=>"12", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"4", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"9", "unique-ip"=>"9", "pdf"=>"5", "year"=>"2010", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"5", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"11", "year"=>"2010", "pdf"=>"3", "unique-ip"=>"11", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"6", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"10", "unique-ip"=>"11", "pdf"=>"5", "year"=>"2010", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"7", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"5", "year"=>"2010", "pdf"=>"1", "unique-ip"=>"5", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"scanned-page-browse"=>"0", "month"=>"8", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"6", "unique-ip"=>"9", "pdf"=>"7", "year"=>"2010", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"9", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"0", "year"=>"2010", "pdf"=>"1", "unique-ip"=>"4", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"10", "cited-by"=>"0", "abstract"=>"4", "full-text"=>"7", "unique-ip"=>"11", "pdf"=>"1", "year"=>"2010", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"11", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"11", "year"=>"2010", "pdf"=>"4", "unique-ip"=>"11", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"12", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"18", "unique-ip"=>"15", "pdf"=>"5", "year"=>"2010", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"1", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"13", "year"=>"2011", "pdf"=>"2", "unique-ip"=>"16", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"2", "cited-by"=>"0", "abstract"=>"5", "full-text"=>"13", "unique-ip"=>"16", "pdf"=>"6", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"3", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"17", "year"=>"2011", "pdf"=>"4", "unique-ip"=>"16", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"4", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"8", "unique-ip"=>"9", "pdf"=>"2", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"5", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"7", "year"=>"2011", "pdf"=>"1", "unique-ip"=>"11", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"6", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"12", "unique-ip"=>"17", "pdf"=>"10", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"7", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"8", "year"=>"2011", "pdf"=>"3", "unique-ip"=>"8", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"8", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"11", "unique-ip"=>"10", "pdf"=>"3", "year"=>"2011", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"9", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"6", "year"=>"2011", "pdf"=>"3", "unique-ip"=>"8", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"10", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"7", "unique-ip"=>"9", "pdf"=>"3", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"11", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"8", "year"=>"2011", "pdf"=>"2", "unique-ip"=>"7", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"12", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"4", "unique-ip"=>"4", "pdf"=>"2", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"1", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"6", "year"=>"2012", "pdf"=>"0", "unique-ip"=>"4", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"2", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"2", "unique-ip"=>"2", "pdf"=>"2", "year"=>"2012", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"3", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"5", "year"=>"2012", "pdf"=>"0", "unique-ip"=>"5", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"4", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"2", "unique-ip"=>"2", "pdf"=>"1", "year"=>"2012", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"5", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"6", "year"=>"2012", "pdf"=>"2", "unique-ip"=>"7", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"84", "month"=>"4", "cited-by"=>"3", "abstract"=>"0", "full-text"=>"1", "unique-ip"=>"17", "pdf"=>"3", "year"=>"2008", "figure"=>"240", "scanned-summary"=>"135", "supp-data"=>"112"}
  • {"month"=>"5", "scanned-page-browse"=>"47", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"2", "year"=>"2008", "pdf"=>"7", "unique-ip"=>"18", "figure"=>"151", "scanned-summary"=>"77", "supp-data"=>"69"}
  • {"scanned-page-browse"=>"0", "month"=>"6", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"63", "unique-ip"=>"56", "pdf"=>"50", "year"=>"2008", "figure"=>"10", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"7", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"45", "year"=>"2008", "pdf"=>"29", "unique-ip"=>"41", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"33", "month"=>"8", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"0", "unique-ip"=>"6", "pdf"=>"3", "year"=>"2008", "figure"=>"86", "scanned-summary"=>"44", "supp-data"=>"34"}
  • {"month"=>"9", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"34", "year"=>"2008", "pdf"=>"15", "unique-ip"=>"32", "figure"=>"11", "scanned-summary"=>"0", "supp-data"=>"3"}
  • {"scanned-page-browse"=>"0", "month"=>"10", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"34", "unique-ip"=>"32", "pdf"=>"18", "year"=>"2008", "figure"=>"14", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"11", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"26", "year"=>"2008", "pdf"=>"18", "unique-ip"=>"20", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"12", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"29", "unique-ip"=>"18", "pdf"=>"18", "year"=>"2008", "figure"=>"9", "scanned-summary"=>"0", "supp-data"=>"3"}
  • {"month"=>"1", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"19", "year"=>"2009", "pdf"=>"12", "unique-ip"=>"18", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"2", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"21", "unique-ip"=>"19", "pdf"=>"8", "year"=>"2009", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"3", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"19", "year"=>"2009", "pdf"=>"9", "unique-ip"=>"19", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"4", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"27", "unique-ip"=>"24", "pdf"=>"11", "year"=>"2009", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"month"=>"5", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"12", "year"=>"2009", "pdf"=>"7", "unique-ip"=>"11", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"6", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"24", "unique-ip"=>"23", "pdf"=>"11", "year"=>"2009", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"7", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"21", "year"=>"2009", "pdf"=>"5", "unique-ip"=>"18", "figure"=>"18", "scanned-summary"=>"0", "supp-data"=>"4"}
  • {"scanned-page-browse"=>"0", "month"=>"8", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"14", "unique-ip"=>"13", "pdf"=>"5", "year"=>"2009", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"9", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"14", "year"=>"2009", "pdf"=>"6", "unique-ip"=>"13", "figure"=>"14", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"10", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"4", "unique-ip"=>"6", "pdf"=>"2", "year"=>"2009", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"11", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"13", "year"=>"2009", "pdf"=>"5", "unique-ip"=>"16", "figure"=>"12", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"12", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"10", "unique-ip"=>"10", "pdf"=>"3", "year"=>"2009", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"4", "year"=>"2012", "pdf"=>"0", "unique-ip"=>"4", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"2", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"8", "pdf"=>"20", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"15", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"1", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"3", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"2", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"4", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"18", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"3", "cited-by"=>"1", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"1", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"15", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"12", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"0", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"15", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"34", "full-text"=>"42", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"18", "supp-data"=>"3", "cited-by"=>"1", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"3", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"0", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}

Relative Metric

{"start_date"=>"2008-01-01T00:00:00Z", "end_date"=>"2008-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[324, 674, 863, 1015, 1146, 1259, 1360, 1452, 1540, 1621, 1704, 1776, 1843, 1905, 1968, 2029, 2097, 2162, 2233, 2289, 2352, 2412, 2476, 2538, 2601, 2655, 2714, 2777, 2841, 2894, 2946, 2998, 3047, 3103, 3151, 3201, 3258, 3317, 3369, 3424, 3471, 3521, 3583, 3625, 3687, 3743, 3788, 3838, 3896, 3953, 4015, 4075, 4148, 4201, 4275, 4347, 4420, 4486, 4535, 4592, 4660, 4731, 4792, 4845, 4901, 4971, 5027, 5089, 5148, 5200, 5261, 5318, 5374]}, {"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[303, 640, 835, 987, 1125, 1245, 1347, 1439, 1513, 1610, 1694, 1763, 1823, 1883, 1949, 2011, 2076, 2137, 2198, 2258, 2326, 2372, 2430, 2512, 2565, 2617, 2681, 2735, 2795, 2850, 2907, 2966, 3033, 3081, 3129, 3177, 3224, 3279, 3334, 3382, 3435, 3489, 3547, 3587, 3634, 3695, 3756, 3810, 3861, 3912, 3984, 4046, 4109, 4169, 4236, 4288, 4346, 4417, 4463, 4536, 4587, 4681, 4731, 4787, 4858, 4910, 4993, 5046, 5100, 5168, 5233, 5283, 5351]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[329, 686, 873, 1009, 1130, 1239, 1337, 1426, 1509, 1585, 1667, 1746, 1816, 1880, 1937, 1999, 2058, 2120, 2179, 2245, 2311, 2374, 2437, 2511, 2565, 2616, 2671, 2723, 2783, 2844, 2902, 2958, 3017, 3056, 3104, 3169, 3206, 3249, 3298, 3354, 3428, 3479, 3526, 3579, 3628, 3679, 3727, 3771, 3819, 3865, 3927, 4006, 4075, 4116, 4164, 4250, 4323, 4380, 4436, 4492, 4547, 4599, 4652, 4708, 4761, 4811, 4889, 4966, 5025, 5071, 5134, 5186, 5258]}, {"subject_area"=>"/Medicine and health sciences/Diagnostic medicine", "average_usage"=>[301, 641, 853, 1045, 1173, 1302, 1416, 1511, 1608, 1708, 1814, 1892, 1956, 2015, 2121, 2200, 2284, 2369, 2436, 2469, 2540, 2602, 2707, 2768, 2834, 2877, 2920, 2975, 3088, 3141, 3190, 3247, 3309, 3360, 3411, 3491, 3562, 3616, 3653, 3699, 3743, 3819, 3868, 3901, 3967, 4018, 4070, 4120, 4170, 4223, 4280, 4322, 4366, 4436, 4480, 4530, 4593, 4643, 4690, 4771, 4845, 4907, 4998, 5071, 5136, 5207, 5277, 5332, 5382, 5418, 5473, 5520, 5552]}]}
Loading … Spinner
There are currently no alerts