Activation of p38MAPK Contributes to Expanded Polyglutamine-Induced Cytotoxicity
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Activation of p38MAPK contributes to expanded polyglutamine-induced cytotoxicity", "type"=>"journal", "authors"=>[{"first_name"=>"Maria", "last_name"=>"Tsirigotis", "scopus_author_id"=>"7004053969"}, {"first_name"=>"R. Mitchell", "last_name"=>"Baldwin", "scopus_author_id"=>"13604667900"}, {"first_name"=>"Matthew Y.", "last_name"=>"Tang", "scopus_author_id"=>"24475922300"}, {"first_name"=>"Ian A J", "last_name"=>"Lorimer", "scopus_author_id"=>"13605710100"}, {"first_name"=>"Douglas A.", "last_name"=>"Gray", "scopus_author_id"=>"7403494201"}], "year"=>2008, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"18461158", "doi"=>"10.1371/journal.pone.0002130", "sgr"=>"47749118777", "isbn"=>"1932-6203 (Electronic) 1932-6203 (Linking)", "scopus"=>"2-s2.0-47749118777", "issn"=>"19326203", "pui"=>"352025176"}, "id"=>"f5e7596d-3fea-318e-a05c-649535c32a03", "abstract"=>"BACKGROUND: The signaling pathways that may modulate the pathogenesis of diseases induced by expanded polyglutamine proteins are not well understood.\\n\\nMETHODOLOGIES/PRINCIPAL FINDINGS: Herein we demonstrate that expanded polyglutamine protein cytotoxicity is mediated primarily through activation of p38MAPK and that the atypical PKC iota (PKCiota) enzyme antagonizes polyglutamine-induced cell death through induction of the ERK signaling pathway. We show that pharmacological blockade of p38MAPK rescues cells from polyglutamine-induced cell death whereas inhibition of ERK recapitulates the sensitivity observed in cells depleted of PKCiota by RNA interference. We provide evidence that two unrelated proteins with expanded polyglutamine repeats induce p38MAPK in cultured cells, and demonstrate induction of p38MAPK in an in vivo model of neurodegeneration (spinocerebellar ataxia 1, or SCA-1).\\n\\nCONCLUSIONS/SIGNIFICANCE: Taken together, our data implicate activated p38MAPK in disease progression and suggest that its inhibition may represent a rational strategy for therapeutic intervention in the polyglutamine disorders.", "link"=>"http://www.mendeley.com/research/activation-p38mapk-contributes-expanded-polyglutamineinduced-cytotoxicity", "reader_count"=>18, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>7, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>1, "Student > Bachelor"=>3, "Professor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>7, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>1, "Student > Bachelor"=>3, "Professor"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>11, "Medicine and Dentistry"=>3, "Neuroscience"=>1, "Chemistry"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Neuroscience"=>{"Neuroscience"=>1}, "Chemistry"=>{"Chemistry"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>11}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}}, "reader_count_by_country"=>{"Canada"=>1, "United States"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/47749118777"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/47749118777?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=47749118777&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=47749118777&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/47749118777", "dc:identifier"=>"SCOPUS_ID:47749118777", "eid"=>"2-s2.0-47749118777", "dc:title"=>"Activation of p38MAPK contributes to expanded polyglutamine-induced cytotoxicity", "dc:creator"=>"Tsirigotis M.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"3", "prism:issueIdentifier"=>"5", "prism:pageRange"=>nil, "prism:coverDate"=>"2008-05-07", "prism:coverDisplayDate"=>"7 May 2008", "prism:doi"=>"10.1371/journal.pone.0002130", "citedby-count"=>"9", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Ottawa Hospital Research Institute", "affiliation-city"=>"Ottawa", "affiliation-country"=>"Canada"}], "pubmed-id"=>"18461158", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e2130", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0002130", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"5", "year"=>"2008", "pdf_views"=>"54", "xml_views"=>"73", "html_views"=>"134"}
  • {"month"=>"6", "year"=>"2008", "pdf_views"=>"10", "xml_views"=>"89", "html_views"=>"39"}
  • {"month"=>"7", "year"=>"2008", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"8", "year"=>"2008", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"9", "year"=>"2008", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"54"}
  • {"month"=>"10", "year"=>"2008", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"11", "year"=>"2008", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"12", "year"=>"2008", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2009", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"2", "year"=>"2009", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"3", "year"=>"2009", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"4", "year"=>"2009", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"5", "year"=>"2009", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"6", "year"=>"2009", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"7", "year"=>"2009", "pdf_views"=>"0", "xml_views"=>"2", "html_views"=>"32"}
  • {"month"=>"8", "year"=>"2009", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"9", "year"=>"2009", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"10", "year"=>"2009", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"11", "year"=>"2009", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"12", "year"=>"2009", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"1", "year"=>"2010", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"2", "year"=>"2010", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"3", "year"=>"2010", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"4", "year"=>"2010", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"5", "year"=>"2010", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"6", "year"=>"2010", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"7", "year"=>"2010", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"8", "year"=>"2010", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"9", "year"=>"2010", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"36"}
  • {"month"=>"10", "year"=>"2010", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"11", "year"=>"2010", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"12", "year"=>"2010", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"1", "year"=>"2011", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"17"}
  • {"month"=>"2", "year"=>"2011", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"3", "year"=>"2011", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"4", "year"=>"2011", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"5", "year"=>"2011", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"6", "year"=>"2011", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"7", "year"=>"2011", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"8", "year"=>"2011", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"9", "year"=>"2011", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"10", "year"=>"2011", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"11", "year"=>"2011", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"5", "html_views"=>"9"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"15"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"22"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"3"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"31"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"28"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"2", "html_views"=>"10"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"3", "html_views"=>"1"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"2", "html_views"=>"2"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"0"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"1"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/932248"], "description"=>"<p>A) U87MG cells transfected with the control or with siPKCιA and siPKCιB were transiently transfected with plasmids encoding GFP, Htt-25 or Htt-103 for 24 hours. Cell morphology was assessed by phase contrast microscopy. An increase in the number of shrunken, rounded and detached cells was observed in PKCι depleted cells expressing Htt-103 when compared to control RNA transfected cells or cells expressing GFP or Htt-25. Magnification was 40×. B) U87MG cells expressing GFP, Htt-25 or Htt-103 for 24 hours were visualized under fluorescence (bottom panel) to assess transfection efficiency. Upper panels represent the same field of view visualized under white light. Arrowheads demonstrate nuclear inclusions in Htt-103 expressing cells. Scale bars represent 100 µm. C) Western blot analysis with a PKCι specific antibody of cell extracts from U87MG cells transfected in duplicate with either the control or siPKCιA and siPKCιB showing the reduction in the protein levels of PKCι at 24 and 48 hours post-transfection. The membrane was re-probed with an antibody directed against Pan-ERK which served as a loading control. D) Western blot analysis of cell extracts from cells transfected with either control or siPKCιA expressing GFP, Htt-25 or Htt-103 with an antibody raised against AFP. No significant difference in the protein levels of GFP, Htt-25 and Htt-103 were observed in extracts from control and PKCι transfected cells. The membrane was re-probed with an antibody directed against Pan-ERK which served as a loading control.</p>", "links"=>[], "tags"=>["alterations", "depleted", "cells", "expressing"], "article_id"=>602689, "categories"=>["Biochemistry", "Neuroscience"], "users"=>["Maria Tsirigotis", "R. Mitchell Baldwin", "Matthew Y. Tang", "Ian A. J. Lorimer", "Douglas A. Gray"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0002130.g001", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Morphological_alterations_in_PKC_953_depleted_cells_expressing_Htt_103_/602689", "title"=>"Morphological alterations in PKCι depleted cells expressing Htt-103.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 10:32:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/932664"], "description"=>"<p>A) Western blot analysis of extracts from control and siPKCιA transfected cells expressing GFP, Htt-25 or Htt-103 with the phospho-38MAPK antibody showing the phosphorylation of p38MAPK in extracts from control and siPKCιA transfected cells expressing Htt-103. Phospho-p38MAPK levels were slightly increased in extracts from cells transfected with Htt-25 when compared to GFP transfectants. The levels of total p38MAPK remained unchanged in all extracts as assessed by Western blot analysis with the p38MAPK antibody. Efficient depletion of PKCι was confirmed by re-probing the membrane with the PKCι specific antibody. B) and C) Control or siPKCιA transfected cells were either left untreated or were pre-treated with p38MAPK inhibitors, SKF86002 (B) and SB202190 (C) for 2 hours prior to transfection with GFP, Htt-25 or Htt-103. 24 hours post-transfection, cell survival was assessed by MTT which revealed an increase in survival of Htt-103 expressing cells by treatment with SKF86002 in both control and siPKCιA transfected cells (** p<0.01). The survival of Htt-103 expressing cells treated with SB202190 was less pronounced when compared to SKF86002 treated cells but was still statistically increased in PKCι depleted cells when compared to the untreated counterparts (* p<0.05). D) Western blot analysis of cell extracts from cells expressing Htt-103 that were either untreated or treated with SKF86002 with the phospho-ATF2 antibody. The analysis revealed an abrogation of ATF2 phosphorylation in Htt-103 expressing cells treated with SKF86002. Re-probing the membrane with an ATF-2 antibody revealed no significant difference in the total levels of ATF2 protein. Actin served as a loading control. E) Flow cytometric analysis of control and siPKCιA transfected cells expressing Htt-103 that were either left untreated or treated with SKF86002. The survival of SKF86002 treated, Htt-103 expressing cells was significantly improved when compared to the untreated counterparts and was comparable to the survival of GFP and Htt-25 transfectants in both the mock and PKCι depleted cells. Data is represented as the average of three independent experiments performed in duplicate with error bars indicating standard error of the mean. F) U87MG cells transfected with GFP, Htt-25 and Htt-103 were treated with SKF86002 in combination with U0126. Survival was assessed by MTT assay 24 hours post-transfection. As described above, treatment of Htt-103 expressing cells with U0126 resulted in reduced viability (* p<0.05) whereas treatment with SKF86002 alone or in combination with U0126 rescued cells from polyglutamine toxicity to a level that was similar to p38MAPK inhibition alone (** p<0.01). Data are represented as the average of three independent experiments performed in triplicate, with error bars indicating standard error of the mean.</p>", "links"=>[], "tags"=>["polyglutamine", "proteins", "induce"], "article_id"=>603109, "categories"=>["Biochemistry", "Neuroscience"], "users"=>["Maria Tsirigotis", "R. Mitchell Baldwin", "Matthew Y. Tang", "Ian A. J. Lorimer", "Douglas A. Gray"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0002130.g004", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expanded_polyglutamine_proteins_induce_p38MAPK_/603109", "title"=>"Expanded polyglutamine proteins induce p38MAPK.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 10:35:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/933111"], "description"=>"<p>Activation p38MAPK signaling is counteracted by PKCι-mediated ERK activation in expanded polyglutamine expressing cells. The pharmacological blockade of ERK with U0126 or by PKCι depletion sensitizes cells to polyglutamine-induced death through a mechanism of exaggerated induction of p38MAPK (A). In contrast, inhibition of p38MAPK phosphorylation by SKF86002 or SB202190 rescues cells from polyglutamine toxicity (B). The blockade of both signaling pathways in cells expressing expanded polyglutamine proteins recapitulates blockade of p38MAPK (B) indicating a causative association between p38MAPK induction and polyglutamine induced death.</p>", "links"=>[], "tags"=>["polyglutamine", "induced"], "article_id"=>603558, "categories"=>["Biochemistry", "Neuroscience"], "users"=>["Maria Tsirigotis", "R. Mitchell Baldwin", "Matthew Y. Tang", "Ian A. J. Lorimer", "Douglas A. Gray"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0002130.g007", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Model_of_polyglutamine_induced_toxicity_/603558", "title"=>"Model of polyglutamine induced toxicity.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 10:37:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/932552"], "description"=>"<p>A) Western blot analysis of cell extracts from control and siPKCιA transfectants with the phospho-ERK specific antibody. The basal levels of ERK phosphoprotein were significantly reduced in PKCι depleted cells when compared to control RNA transfectants. Total ERK levels were assessed with the pan ERK antibody which also served as a loading control. B) U87MG cells were pre-treated with the MEK inhibitor U0126 for 2 hours prior to transfection with GFP, Htt-25 and Htt-103. Cell survival was assessed by MTT 24 hours post-transfection. Blockade of ERK in Htt-103 expressing cells resulted in a significant reduction in cell survival when compared to untreated Htt-103 expressing cells (* p<0.05). Data are represented as the average of three independent experiments performed in triplicate, with error bars indicating standard error of the mean. C) Western blot analysis of extracts from untreated and U0126 treated U87MG cells expressing Htt-103 with the phospho-ERK antibody confirming the blockade of ERK phosphorylation in U0126 treated cells. Pan-ERK was used to detect total ERK levels and actin served as a loading control.</p>", "links"=>[], "tags"=>["erk", "activation", "protects", "cells", "expanded", "polyglutamine-induced"], "article_id"=>602997, "categories"=>["Biochemistry", "Neuroscience"], "users"=>["Maria Tsirigotis", "R. Mitchell Baldwin", "Matthew Y. Tang", "Ian A. J. Lorimer", "Douglas A. Gray"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0002130.g003", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_PKC_953_mediated_ERK_activation_protects_cells_from_expanded_polyglutamine_induced_cytotoxicity_/602997", "title"=>"PKCι-mediated ERK activation protects cells from expanded polyglutamine-induced cytotoxicity.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 10:34:22"}
  • {"files"=>["https://ndownloader.figshare.com/files/932826"], "description"=>"<p>A) Western blot analysis of duplicate cell extracts from U87MG cells expressing Atx-30 and Atx-83 with the phospho-p38MAPK antibody. An increase in phosphorylated p38MAPK was observed in lysates from Atx-83 expressing cells when compared to lysates from mock transfected or cells expressing Atx-30. Total levels of p38MAPK were increased in lysates from cells transfected with either Atx-30 and Atx-83 when compared to mock transfected cells as assessed by re-probing of the membrane with the p38MAPK antibody. Actin served as a loading control. B) HT4 and NIH-3T3 cells were transiently transfected in duplicate with the Atx-83 expression construct. Cell extracts were analyzed by western blot analysis with the phospho-p38MAPK antibody. p38MAPK activation was observed in cell extracts from both NIH-3T3 and HT4 cells expressing Atx-83. The induction of p38MAPK was not observed in lysates from cells expressing an empty vector control. C) Untreated or SKF86002 treated control or siPKCιA transfected cells were transfected with Atx-30 or Atx-83 for 24 hours and cell survival was assessed by MTT. The analysis revealed a decrease in survival of Atx-83 expressing cells in control RNA transfected cells that was significantly more pronounced by PKCι depletion. Blockade of p38MAPK increased survival of Atx-83 expressing cells in both control and siPKCι transfectants such that it was comparable to the survival of Atx-30 expressing cells. Data represent the average of three independent experiments performed in triplicate, with error bars indicating standard error of the mean (* p<0.05). D) U87MG cells were co-transfected with Htt-103 and either empty vector alone or expression constructs encoding wild-type (wt p38) or dominant-negative (KD) p38MAPK alpha. 24 hours post-transfection, cells were analyzed by MTT which revealed a statistically significant increase in survival in cells co-expressing Htt-103 and dominant-negative p38MAPK alpha (** p<0.01). The survival of cells expressing empty vector control alone or co-expressing Htt-25 with empty vector was not significantly different. E) Western blot analysis of extracts from Atx-30 and Atx-83 transfected cells with an ataxin-1 specific antibody revealing a similar level of expression. Actin served as a loading control.</p>", "links"=>[], "tags"=>["ataxin-1", "toxicity", "mediated", "induction"], "article_id"=>603272, "categories"=>["Biochemistry", "Neuroscience"], "users"=>["Maria Tsirigotis", "R. Mitchell Baldwin", "Matthew Y. Tang", "Ian A. J. Lorimer", "Douglas A. Gray"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0002130.g005", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expanded_ataxin_1_toxicity_is_mediated_through_induction_of_p38MAPK_/603272", "title"=>"Expanded ataxin-1 toxicity is mediated through induction of p38MAPK.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 10:35:58"}
  • {"files"=>["https://ndownloader.figshare.com/files/933025"], "description"=>"<p>A) and B) Western blot analysis of cerebellar extracts from 3 month-old nontransgenic, A02 and B05 mice with the phospho-p38MAPK antibody. An increase in the protein levels of phospho-p38 was detected in the extracts from B05 mice when compared to A02 and nontransgenic control extracts. Total p38MAPK levels were similar as assessed by re-probing the membrane with the p38MAPK antibody or actin. C) Western blot analysis of cerebellar extracts from B05 mice at 1,2 and 3 months of age with the phospho-p38MAPK antibody. The analysis revealed a detectable induction in p38MAPK activation in lysates from mice at 3 month of age. Total levels of p38MAPK were assayed by re-probing the membrane with the p38MAPK antibody which also served as a loading control. D) Immunohistochemistry of mouse cerebella with the phospho-p38MAPK antibody. Panel i) cerebellum of a B05 animal and panel ii) cerebellum of a nontransgenic animal. Immunoreactivity was observed in the cytoplasm and nucleus of Purkinje neurons in B05 and nontransgenic mice. Panel iii) section from a B05 animal stained with the secondary antibody alone demonstrating the absence of immunoreactivity by omission of the primary antibody. Scale bars represent 25 µm.</p>", "links"=>[], "tags"=>["vivo", "induction", "p38mapk", "sca-1"], "article_id"=>603466, "categories"=>["Biochemistry", "Neuroscience"], "users"=>["Maria Tsirigotis", "R. Mitchell Baldwin", "Matthew Y. Tang", "Ian A. J. Lorimer", "Douglas A. Gray"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0002130.g006", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_In_vivo_induction_of_p38MAPK_in_SCA_1_mice_/603466", "title"=>"In vivo induction of p38MAPK in SCA-1 mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 10:37:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/932384"], "description"=>"<p>A) and B) U87MG cells transfected for 24 hours with either control or siPKCιA and PKCιB were plated in 96 well dishes. Subsequently, cells were transfected with expression constructs encoding GFP, Htt-25 (A) and Htt-103 (B) and cell survival was measured by MTT assay. A) The survival of mock RNA transfected cells expressing Htt-25 was comparable to cells expressing GFP alone. A slight decrease in cell survival was observed in PKCι depleted cells expressing Htt-25 when compared to GFP transfectants (* p<0.05). B) A marked decrease in cell survival was observed in control RNA transfected cells expressing Htt-103 that was further pronounced in PKCι depleted cells (* p<0.05). C) Survival as measured by trypan blue exclusion of U87MG cells transfected with control or siPKCιA in the presence or absence of Htt-103. In accordance with the MTT assay, an increase in the population of dead cells was observed in control transfected U87MG cells expressing Htt-103 which was further increased in PKCι depleted cells. D) Flow cytometric analysis of cells transfected with either the control or siPKCι expressing Htt-103 showing an increase in the number of dead cells in PKCι depleted cells when compared to control transfected cells. Data represents the average of three independent experiments, with error bars indicating standard deviation (** p<0.01). E) U87MG cells stably expressing flag epitope tagged PKCι (ftPKCι) were transiently transfected with the Htt-103 expression construct for 24 hours. Survival as assessed by MTT analysis revealed a modest increase in survival in PKCι transfectants when compared to the parental U87MG cells (** p<0.01). F) Western blot analysis of triplicate cell extracts from untransfected U87MG and cells stably expressing flag-tagged PKCι with antibodies raised against PKCι and the flag epitope tag respectively. The flag tag specific antibody detected ectopically expressed PKCι in transfected cells, which was absent in the untransfected control cell extracts. The PKCι antibody detected endogenous and exogenous PKCι in lysates from U87MG cells and cells stably expressing PKCι. Pan-ERK served as the loading control. MTT and trypan blue data are represented as the average of three independent experiments performed in triplicate, with errors bars indicating standard error of the mean.</p>", "links"=>[], "tags"=>["sensitizes", "cells", "polyglutamine", "induced"], "article_id"=>602831, "categories"=>["Biochemistry", "Neuroscience"], "users"=>["Maria Tsirigotis", "R. Mitchell Baldwin", "Matthew Y. Tang", "Ian A. J. Lorimer", "Douglas A. Gray"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0002130.g002", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Depletion_of_PKC_953_sensitizes_cells_to_polyglutamine_induced_toxicity_/602831", "title"=>"Depletion of PKCι sensitizes cells to polyglutamine induced toxicity.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 10:33:20"}

PMC Usage Stats | Further Information

  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"12", "unique-ip"=>"10", "pdf"=>"7", "year"=>"2010", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"16", "year"=>"2010", "pdf"=>"14", "unique-ip"=>"14", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"4", "full-text"=>"10", "unique-ip"=>"12", "pdf"=>"5", "year"=>"2010", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"10", "year"=>"2010", "pdf"=>"4", "unique-ip"=>"10", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"4", "unique-ip"=>"6", "pdf"=>"4", "year"=>"2010", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"2", "year"=>"2010", "pdf"=>"2", "unique-ip"=>"6", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"2", "unique-ip"=>"5", "pdf"=>"3", "year"=>"2010", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"4", "year"=>"2010", "pdf"=>"4", "unique-ip"=>"5", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"7", "full-text"=>"1", "unique-ip"=>"8", "pdf"=>"0", "year"=>"2010", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"4", "full-text"=>"9", "year"=>"2010", "pdf"=>"11", "unique-ip"=>"16", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"5", "unique-ip"=>"7", "pdf"=>"3", "year"=>"2010", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"6", "year"=>"2010", "pdf"=>"3", "unique-ip"=>"7", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"2", "unique-ip"=>"3", "pdf"=>"0", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"4", "year"=>"2011", "pdf"=>"1", "unique-ip"=>"5", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"10", "unique-ip"=>"11", "pdf"=>"2", "year"=>"2011", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"6", "year"=>"2011", "pdf"=>"4", "unique-ip"=>"9", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"6", "unique-ip"=>"9", "pdf"=>"1", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"4", "year"=>"2011", "pdf"=>"7", "unique-ip"=>"11", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"0", "unique-ip"=>"1", "pdf"=>"0", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"3", "year"=>"2011", "pdf"=>"0", "unique-ip"=>"4", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"3", "unique-ip"=>"5", "pdf"=>"2", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"2", "year"=>"2011", "pdf"=>"0", "unique-ip"=>"4", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"4", "unique-ip"=>"4", "pdf"=>"1", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"1", "year"=>"2011", "pdf"=>"0", "unique-ip"=>"1", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"29", "unique-ip"=>"15", "pdf"=>"3", "year"=>"2012", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"31", "year"=>"2012", "pdf"=>"3", "unique-ip"=>"13", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"5", "unique-ip"=>"8", "pdf"=>"3", "year"=>"2012", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"1", "year"=>"2012", "pdf"=>"0", "unique-ip"=>"1", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"4", "unique-ip"=>"5", "pdf"=>"2", "year"=>"2012", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"5", "scanned-page-browse"=>"41", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"2", "year"=>"2008", "pdf"=>"0", "unique-ip"=>"17", "figure"=>"120", "scanned-summary"=>"60", "supp-data"=>"54"}
  • {"scanned-page-browse"=>"0", "month"=>"6", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"37", "unique-ip"=>"37", "pdf"=>"25", "year"=>"2008", "figure"=>"6", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"7", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"31", "year"=>"2008", "pdf"=>"12", "unique-ip"=>"27", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"11", "month"=>"8", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"0", "unique-ip"=>"1", "pdf"=>"0", "year"=>"2008", "figure"=>"29", "scanned-summary"=>"17", "supp-data"=>"12"}
  • {"month"=>"9", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"4", "full-text"=>"31", "year"=>"2008", "pdf"=>"17", "unique-ip"=>"30", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"10", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"23", "unique-ip"=>"24", "pdf"=>"10", "year"=>"2008", "figure"=>"6", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"11", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"27", "year"=>"2008", "pdf"=>"19", "unique-ip"=>"27", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"12", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"13", "unique-ip"=>"12", "pdf"=>"5", "year"=>"2008", "figure"=>"6", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"1", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"10", "year"=>"2009", "pdf"=>"8", "unique-ip"=>"10", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"2", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"22", "unique-ip"=>"22", "pdf"=>"14", "year"=>"2009", "figure"=>"13", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"3", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"12", "year"=>"2009", "pdf"=>"7", "unique-ip"=>"12", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"4", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"15", "unique-ip"=>"14", "pdf"=>"13", "year"=>"2009", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"5", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"14", "year"=>"2009", "pdf"=>"0", "unique-ip"=>"10", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"6", "cited-by"=>"0", "abstract"=>"4", "full-text"=>"19", "unique-ip"=>"17", "pdf"=>"10", "year"=>"2009", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"7", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"8", "year"=>"2009", "pdf"=>"4", "unique-ip"=>"11", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"8", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"16", "unique-ip"=>"18", "pdf"=>"13", "year"=>"2009", "figure"=>"11", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"9", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"20", "year"=>"2009", "pdf"=>"7", "unique-ip"=>"16", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"10", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"3", "unique-ip"=>"5", "pdf"=>"3", "year"=>"2009", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"11", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"8", "year"=>"2009", "pdf"=>"9", "unique-ip"=>"13", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"12", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"6", "unique-ip"=>"10", "pdf"=>"7", "year"=>"2009", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"4", "year"=>"2012", "pdf"=>"0", "unique-ip"=>"4", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"15", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"1", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"3", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"18", "full-text"=>"19", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"10", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2008-01-01T00:00:00Z", "end_date"=>"2008-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[324, 674, 863, 1015, 1146, 1259, 1360, 1452, 1540, 1621, 1704, 1776, 1843, 1905, 1968, 2029, 2097, 2162, 2233, 2289, 2352, 2412, 2476, 2538, 2601, 2655, 2714, 2777, 2841, 2894, 2946, 2998, 3047, 3103, 3151, 3201, 3258, 3317, 3369, 3424, 3471, 3521, 3583, 3625, 3687, 3743, 3788, 3838, 3896, 3953, 4015, 4075, 4148, 4201, 4275, 4347, 4420, 4486, 4535, 4592, 4660, 4731, 4792, 4845, 4901, 4971, 5027, 5089, 5148, 5200, 5261, 5318, 5374]}, {"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[303, 640, 835, 987, 1125, 1245, 1347, 1439, 1513, 1610, 1694, 1763, 1823, 1883, 1949, 2011, 2076, 2137, 2198, 2258, 2326, 2372, 2430, 2512, 2565, 2617, 2681, 2735, 2795, 2850, 2907, 2966, 3033, 3081, 3129, 3177, 3224, 3279, 3334, 3382, 3435, 3489, 3547, 3587, 3634, 3695, 3756, 3810, 3861, 3912, 3984, 4046, 4109, 4169, 4236, 4288, 4346, 4417, 4463, 4536, 4587, 4681, 4731, 4787, 4858, 4910, 4993, 5046, 5100, 5168, 5233, 5283, 5351]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[303, 628, 827, 976, 1105, 1222, 1321, 1406, 1482, 1564, 1639, 1713, 1784, 1851, 1915, 1966, 2020, 2080, 2138, 2203, 2258, 2315, 2379, 2437, 2492, 2556, 2613, 2670, 2726, 2785, 2834, 2886, 2948, 2988, 3037, 3089, 3140, 3179, 3236, 3283, 3340, 3390, 3438, 3487, 3541, 3586, 3642, 3707, 3754, 3805, 3853, 3897, 3956, 4016, 4081, 4152, 4218, 4258, 4319, 4378, 4442, 4487, 4536, 4589, 4635, 4689, 4754, 4807, 4863, 4920, 4982, 5042, 5094]}]}

F1000Prime | Further Information

Loading … Spinner
There are currently no alerts