Fluoromycobacteriophages for Rapid, Specific, and Sensitive Antibiotic Susceptibility Testing of Mycobacterium tuberculosis
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Fluoromycobacteriophages for rapid, specific, and sensitive antibiotic susceptibility testing of Mycobacterium tuberculosis", "type"=>"journal", "authors"=>[{"first_name"=>"Mariana", "last_name"=>"Piuri", "scopus_author_id"=>"15074283700"}, {"first_name"=>"William R.", "last_name"=>"Jacobs", "scopus_author_id"=>"10240378100"}, {"first_name"=>"Graham F.", "last_name"=>"Hatfull", "scopus_author_id"=>"7007104717"}], "year"=>2009, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"354416627", "sgr"=>"63749110948", "issn"=>"19326203", "pmid"=>"19300517", "scopus"=>"2-s2.0-63749110948", "doi"=>"10.1371/journal.pone.0004870", "isbn"=>"1932-6203"}, "id"=>"c9f25062-a836-363e-89cd-4b06cfb159a5", "abstract"=>"Rapid antibiotic susceptibility testing of Mycobacterium tuberculosis is of paramount importance as multiple- and extensively-drug resistant strains of M. tuberculosis emerge and spread. We describe here a virus-based assay in which fluoromycobacteriophages are used to deliver a GFP or ZsYellow fluorescent marker gene to M. tuberculosis, which can then be monitored by fluorescent detection approaches including fluorescent microscopy and flow cytometry. Pre-clinical evaluations show that addition of either Rifampicin or Streptomycin at the time of phage addition obliterates fluorescence in susceptible cells but not in isogenic resistant bacteria enabling drug sensitivity determination in less than 24 hours. Detection requires no substrate addition, fewer than 100 cells can be identified, and resistant bacteria can be detected within mixed populations. Fluorescence withstands fixation by paraformaldehyde providing enhanced biosafety for testing MDR-TB and XDR-TB infections.", "link"=>"http://www.mendeley.com/research/fluoromycobacteriophages-rapid-specific-sensitive-antibiotic-susceptibility-testing-mycobacterium-tu-5", "reader_count"=>66, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>3, "Researcher"=>17, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>15, "Student > Postgraduate"=>2, "Student > Master"=>13, "Other"=>1, "Student > Bachelor"=>6, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>3, "Researcher"=>17, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>15, "Student > Postgraduate"=>2, "Student > Master"=>13, "Other"=>1, "Student > Bachelor"=>6, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>10, "Agricultural and Biological Sciences"=>38, "Medicine and Dentistry"=>8, "Arts and Humanities"=>1, "Physics and Astronomy"=>1, "Immunology and Microbiology"=>4}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>8}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>4}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>38}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>10}, "Unspecified"=>{"Unspecified"=>3}, "Environmental Science"=>{"Environmental Science"=>1}, "Arts and Humanities"=>{"Arts and Humanities"=>1}}, "reader_count_by_country"=>{"United States"=>1, "Chile"=>1, "India"=>1}, "group_count"=>3}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/63749110948"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/63749110948?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=63749110948&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=63749110948&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/63749110948", "dc:identifier"=>"SCOPUS_ID:63749110948", "eid"=>"2-s2.0-63749110948", "dc:title"=>"Fluoromycobacteriophages for rapid, specific, and sensitive antibiotic susceptibility testing of Mycobacterium tuberculosis", "dc:creator"=>"Piuri M.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"4", "prism:issueIdentifier"=>"3", "prism:pageRange"=>nil, "prism:coverDate"=>"2009-03-20", "prism:coverDisplayDate"=>"20 March 2009", "prism:doi"=>"10.1371/journal.pone.0004870", "citedby-count"=>"59", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"University of Pittsburgh", "affiliation-city"=>"Pittsburgh", "affiliation-country"=>"United States"}], "pubmed-id"=>"19300517", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e4870", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0004870", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"3", "year"=>"2009", "pdf_views"=>"188", "xml_views"=>"3", "html_views"=>"484"}
  • {"month"=>"4", "year"=>"2009", "pdf_views"=>"62", "xml_views"=>"0", "html_views"=>"151"}
  • {"month"=>"5", "year"=>"2009", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"6", "year"=>"2009", "pdf_views"=>"51", "xml_views"=>"1", "html_views"=>"106"}
  • {"month"=>"7", "year"=>"2009", "pdf_views"=>"32", "xml_views"=>"1", "html_views"=>"82"}
  • {"month"=>"8", "year"=>"2009", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"9", "year"=>"2009", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"10", "year"=>"2009", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"42"}
  • {"month"=>"11", "year"=>"2009", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"12", "year"=>"2009", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"1", "year"=>"2010", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"2", "year"=>"2010", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"3", "year"=>"2010", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"4", "year"=>"2010", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"5", "year"=>"2010", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"43"}
  • {"month"=>"6", "year"=>"2010", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"7", "year"=>"2010", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"8", "year"=>"2010", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"9", "year"=>"2010", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"49"}
  • {"month"=>"10", "year"=>"2010", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"48"}
  • {"month"=>"11", "year"=>"2010", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"12", "year"=>"2010", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"35"}
  • {"month"=>"1", "year"=>"2011", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"26"}
  • {"month"=>"2", "year"=>"2011", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"3", "year"=>"2011", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"4", "year"=>"2011", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"68"}
  • {"month"=>"5", "year"=>"2011", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"51"}
  • {"month"=>"6", "year"=>"2011", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"7", "year"=>"2011", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"8", "year"=>"2011", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"56"}
  • {"month"=>"9", "year"=>"2011", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"48"}
  • {"month"=>"10", "year"=>"2011", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"11", "year"=>"2011", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"53"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"26", "xml_views"=>"1", "html_views"=>"47"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"52"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"5", "html_views"=>"25"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"78"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"49"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"79"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"68"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"59"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"69"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"76"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"32"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"34"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"81"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"4", "html_views"=>"54"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"38"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"53"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"2", "html_views"=>"74"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"38"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"51"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"37"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"74"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"130"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"131"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"237"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"104"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"99"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"35"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"43"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"47"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"34"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"48"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"47"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"51"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"4", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"15"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"2", "html_views"=>"10"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"3", "html_views"=>"5"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"7"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"26", "xml_views"=>"2", "html_views"=>"29"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"20", "xml_views"=>"3", "html_views"=>"13"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"23"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"10"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/905291"], "description"=>"<p><i>M. tuberculosis</i> was infected with phages as follows: A, phAE87::<i>hsp60-EGFP</i>; C, phAE87::<i>hsp60-ZsYellow</i>; B and D, mock infected controls. Cells were fixed with 2% paraformaldehyde, washed, and resuspended in PBS prior to microscopy. Left panels, fluorescence micrograph images; right panels, merged fluorescence and phase contrast images. Scale bar, 10 µm.</p>", "links"=>[], "tags"=>["cells"], "article_id"=>575755, "categories"=>["Microbiology", "Infectious Diseases"], "users"=>["Mariana Piuri", "William R. Jacobs Jr.", "Graham F. Hatfull"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0004870.g006", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Infection_of_M_tuberculosis_mc_2_6230_cells_with_fluoromycobacteriophages_/575755", "title"=>"Infection of <i>M. tuberculosis</i> mc<sup>2</sup>6230 cells with fluoromycobacteriophages.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-03-20 01:35:55"}
  • {"files"=>["https://ndownloader.figshare.com/files/905495"], "description"=>"<p>A. <i>M. tuberculosis</i> mc<sup>2</sup>6230 strains grown in the absence of Tween detergent were infected with phAE87::<i>hsp60-EGFP</i> with or without antibiotic treatment, fixed with paraformaldehyde, and analyzed by flow cytometry. In panels a, mock-infected control cells and b, phage infected cells, the intensity of fluorescence is plotted against light side scatter. The signal observed in mock-infected cells corresponds to autofluorescence of <i>M. tuberculosis</i> and the shift towards greater fluorescence in phage-infected cells shows that a high proportion of cells are infected and expressed GFP. In panels c–i the level of fluorescence is plotted against the number of events counted. In each experiment mock-infected cells are shown in red, phage infected cells in green, and antibiotic-treated phage infected cells in blue. When treated with rifampicin (Rif) or streptomycin (Str) antibiotics were added simultaneously with phage (φ), incubated for 16 hours, fixed and analyzed; for INH treatment cells were pre-incubated with antibiotic for 48 hours, incubated with phage for 16 hours, fixed, and analyzed. B. <i>M. tuberculosis</i> mc<sup>2</sup>6230 strains were grown in the presence of Tween, washed, preincubated with isoniazid for 24 hours, infected with phAE87::<i>hsp60-EGFP</i>, fixed, and analyzed by flow cytometry. Panels a–e correspond to panels a–c, f, and i of <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004870#pone-0004870-g008\" target=\"_blank\">Figure 8A</a> respectively.</p>", "links"=>[], "tags"=>["susceptibility"], "article_id"=>575960, "categories"=>["Microbiology", "Infectious Diseases"], "users"=>["Mariana Piuri", "William R. Jacobs Jr.", "Graham F. Hatfull"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0004870.g008", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Fluoromycobacteriophage_M_tuberculosis_susceptibility_testing_using_flow_cytometry_/575960", "title"=>"Fluoromycobacteriophage <i>M. tuberculosis</i> susceptibility testing using flow cytometry.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-03-20 01:39:20"}
  • {"files"=>["https://ndownloader.figshare.com/files/905107"], "description"=>"<p>A. A mixture of <i>E. coli</i> cells expressing DsRed-Express and <i>M. smegmatis</i> were infected with (a) phAE87::<i>hsp60-EGFP</i> or (b) mock infected. From left to right: fluorescence micrograph images using a ZsGreen1 filter (green); fluorescence micrograph images using a HQ:R NX filter (red), merged fluorescence images; phase contrast images. Red cells: <i>E. coli</i>; green cells: <i>M. smegmatis</i> mc<sup>2</sup>155 infected with pHAE87::<i>hsp60-EGFP</i>. Scale bar, 10 µm. B. <i>M. smegmatis</i> mc<sup>2</sup>155 cells were infected with phAE87::<i>hsp60-EGFP</i> at different multiplicities of infection (moi). Green cells were counted in 15 individual fields and the percentage of fluorescent cells compared to the total number of cells in phase contrast images determined.</p>", "links"=>[], "tags"=>["fluoromycobacteriophage"], "article_id"=>575569, "categories"=>["Microbiology", "Infectious Diseases"], "users"=>["Mariana Piuri", "William R. Jacobs Jr.", "Graham F. Hatfull"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0004870.g003", "stats"=>{"downloads"=>2, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Specificity_and_efficiency_of_fluoromycobacteriophage_infections_/575569", "title"=>"Specificity and efficiency of fluoromycobacteriophage infections.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-03-20 01:32:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/905235"], "description"=>"<p><i>M. smegmatis</i> mc<sup>2</sup>155 cells were infected with phAE87::<i>hsp60-EGFP</i> and fluorescence detected in A, live cells, B, fixed cells and C, fixed cells after 2 weeks at 4°C. Top, fluorescence micrograph images; bottom, merge of fluorescence and phase contrast images. Scale bar, 10 µm.</p>", "links"=>[], "tags"=>["fixation", "fluoromycobacteriophage-infected"], "article_id"=>575691, "categories"=>["Microbiology", "Infectious Diseases"], "users"=>["Mariana Piuri", "William R. Jacobs Jr.", "Graham F. Hatfull"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0004870.g005", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Paraformaldhyde_fixation_of_fluoromycobacteriophage_infected_mycobacteria_/575691", "title"=>"Paraformaldhyde fixation of fluoromycobacteriophage-infected mycobacteria.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-03-20 01:34:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/904948"], "description"=>"<p>Schematic representation of phAE87::<i>hsp60-EGFP</i> construction. Shuttle phasmid phAE87 is a conditionally replicating derivative of phage TM4 in which the cosmid moiety is flanked by Pac I restriction sites. A plasmid derivative of pYUB854 containing the EGFP gene (pMP14) was used to replace the cosmid in phAE87 followed by lambda packaging and recovery in <i>E. coli</i>.</p>", "links"=>[], "tags"=>["Infectious diseases", "microbiology", "infectious diseases/bacterial infections"], "article_id"=>575407, "categories"=>["Microbiology", "Infectious Diseases"], "users"=>["Mariana Piuri", "William R. Jacobs Jr.", "Graham F. Hatfull"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0004870.g001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Fluoromycobacteriophages_construction_/575407", "title"=>"Fluoromycobacteriophages construction.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-03-20 01:30:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/905174"], "description"=>"<p>Varying numbers of <i>M. smegmatis</i> mc<sup>2</sup>155 cells were infected with pHAE87::hsp60-EGFP at a moi of 100. After infection at 37°C for 4 hrs, cells were concentrated by filtration and visualize using by fluorescence microscopy. A, 10<sup>6</sup>; B,10<sup>5</sup>; C, 10<sup>4</sup>; D, 10<sup>3</sup> and E, 10<sup>2</sup> cells. Scale bar, 20 µm.</p>", "links"=>[], "tags"=>["Infectious diseases", "microbiology", "infectious diseases/bacterial infections"], "article_id"=>575633, "categories"=>["Microbiology", "Infectious Diseases"], "users"=>["Mariana Piuri", "William R. Jacobs Jr.", "Graham F. Hatfull"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0004870.g004", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sensitivity_of_Fluoromycobacteriophages_/575633", "title"=>"Sensitivity of Fluoromycobacteriophages.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-03-20 01:33:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/905626"], "description"=>"<p><i>M. tuberculosis</i> mc<sup>2</sup>6230 <i>wt</i> was mixed with the isogenic Rif<sup>R</sup> strain at different proportions. A total of 10<i><sup>6</sup></i> mixed cells were infected with phAE87::<i>hsp60-EGFP</i> in the presence of Rifampicin; cells were fixed, collected by filtration and analyzed by fluorescent microscopy. (A) 100% <i>wt</i>: 0% Rif<sup>R</sup> (B) 50% <i>wt</i>: 50% Rif<sup>R</sup>; (C) 75% <i>wt</i> :25% Rif<sup>R</sup> ; (D) 90% <i>wt</i> :10% Rif<sup>R</sup>; (E) 99% <i>wt</i> :1% Rif<sup>R</sup>. Left panels, fluorescence micrograph images; scale bar 20 µm; right panels, fluorescence micrograph images, scale bar 10 µm.</p>", "links"=>[], "tags"=>["antibiotic", "resistant"], "article_id"=>576088, "categories"=>["Microbiology", "Infectious Diseases"], "users"=>["Mariana Piuri", "William R. Jacobs Jr.", "Graham F. Hatfull"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0004870.g009", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Infection_of_a_mixed_culture_of_antibiotic_resistant_and_sensitive_strains_/576088", "title"=>"Infection of a mixed culture of antibiotic resistant and sensitive strains.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-03-20 01:41:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/905367"], "description"=>"<p><i>M. tuberculosis</i> mc<sup>2</sup>6230 <i>wt</i> and antibiotic resistant strains were infected with phAE87::<i>hsp60-EGFP</i> with or without antibiotic addition as indicated. A. Cultures were grown in the absence of Tween, washed, and tested. In panels a–d antibiotics and phage were added simultaneously, incubated for 16 hours, and examined by microscopy. In panels e–f INH was added first for 48 hours followed by phage addition, incubation for a further 16 hours, and examined by microscopy. Scale bar, 10 µm. B. Cultures were grown in the presence of Tween, centrifuged and washed twice, and then incubated with antibiotic (as indicated) for 24 hours. Phage were then added, cultures incubated for 16 hour, and examined by microscopy. Scale bar, 20 µm.</p>", "links"=>[], "tags"=>["susceptibility"], "article_id"=>575834, "categories"=>["Microbiology", "Infectious Diseases"], "users"=>["Mariana Piuri", "William R. Jacobs Jr.", "Graham F. Hatfull"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0004870.g007", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Antibiotic_susceptibility_testing_of_M_tuberculosis_using_fluoromycobacteriophages_/575834", "title"=>"Antibiotic susceptibility testing of <i>M. tuberculosis</i> using fluoromycobacteriophages.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-03-20 01:37:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/905022"], "description"=>"<p>Infection of <i>M. smegmatis</i> with A, phAE87::<i>hsp60-EGFP</i>; C, phAE87::<i>hsp60-ZsYellow</i>, or B and D, mock infected controls. Left, fluorescence micrograph images; right, merged fluorescence and phase contrast images. Scale bar, 10 µm.</p>", "links"=>[], "tags"=>["cells"], "article_id"=>575484, "categories"=>["Microbiology", "Infectious Diseases"], "users"=>["Mariana Piuri", "William R. Jacobs Jr.", "Graham F. Hatfull"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0004870.g002", "stats"=>{"downloads"=>1, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Detection_of_M_smegmatis_mc_2_155_cells_with_fluoromycobacteriophages_/575484", "title"=>"Detection of <i>M. smegmatis</i> mc<sup>2</sup>155 cells with fluoromycobacteriophages.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-03-20 01:31:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/905723"], "description"=>"<p><i>M. tuberculosis</i> mc<sup>2</sup>6230 <i>wt</i> was mixed with the isogenic rif<sup>R</sup> strain at different proportions. A total of 10<i><sup>7</sup></i> mixed cells were infected with phAE87::<i>hsp60-EGFP</i> in the presence of Rifampicin; cells were fixed and analyze by flow cytometry. (A) 100% <i>wt</i>: 0% Rif<sup>R</sup> (B) 50% <i>wt</i>: 50% Rif<sup>R</sup>; (C) 75% <i>wt</i> : 25% Rif<sup>R</sup> ; (D) 90% <i>wt</i> :10% Rif<sup>R</sup>; (E) 99% <i>wt</i> :1% Rif<sup>R</sup> . Left panels, the intensity of fluorescence is plotted against light side scatter; right panels, the level of fluorescence is plotted against the number of events counted.</p>", "links"=>[], "tags"=>["cells"], "article_id"=>576186, "categories"=>["Microbiology", "Infectious Diseases"], "users"=>["Mariana Piuri", "William R. Jacobs Jr.", "Graham F. Hatfull"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0004870.g010", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Detection_of_M_tuberculosis_mc_2_6230_Rif_R_cells_in_a_mixed_population_using_flow_cytometry_/576186", "title"=>"Detection of <i>M. tuberculosis</i> mc<sup>2</sup>6230 Rif<sup>R</sup> cells in a mixed population using flow cytometry.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-03-20 01:43:06"}
  • {"files"=>["https://ndownloader.figshare.com/files/447034", "https://ndownloader.figshare.com/files/447047", "https://ndownloader.figshare.com/files/447060", "https://ndownloader.figshare.com/files/447081"], "description"=>"<div><p>Rapid antibiotic susceptibility testing of <em>Mycobacterium tuberculosis</em> is of paramount importance as multiple- and extensively- drug resistant strains of <em>M. tuberculosis</em> emerge and spread. We describe here a virus-based assay in which fluoromycobacteriophages are used to deliver a GFP or ZsYellow fluorescent marker gene to <em>M. tuberculosis</em>, which can then be monitored by fluorescent detection approaches including fluorescent microscopy and flow cytometry. Pre-clinical evaluations show that addition of either Rifampicin or Streptomycin at the time of phage addition obliterates fluorescence in susceptible cells but not in isogenic resistant bacteria enabling drug sensitivity determination in less than 24 hours. Detection requires no substrate addition, fewer than 100 cells can be identified, and resistant bacteria can be detected within mixed populations. Fluorescence withstands fixation by paraformaldehyde providing enhanced biosafety for testing MDR-TB and XDR-TB infections.</p></div>", "links"=>[], "tags"=>["fluoromycobacteriophages", "antibiotic", "susceptibility"], "article_id"=>148126, "categories"=>["Cancer", "Microbiology"], "users"=>["Mariana Piuri", "William R. Jacobs Jr.", "Graham F. Hatfull"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0004870.s001", "https://dx.doi.org/10.1371/journal.pone.0004870.s002", "https://dx.doi.org/10.1371/journal.pone.0004870.s003", "https://dx.doi.org/10.1371/journal.pone.0004870.s004"], "stats"=>{"downloads"=>38, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Fluoromycobacteriophages_for_Rapid_Specific_and_Sensitive_Antibiotic_Susceptibility_Testing_of_Mycobacterium_tuberculosis_/148126", "title"=>"Fluoromycobacteriophages for Rapid, Specific, and Sensitive Antibiotic Susceptibility Testing of <em>Mycobacterium tuberculosis</em>", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2009-03-20 02:15:26"}

PMC Usage Stats | Further Information

  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"19", "unique-ip"=>"26", "pdf"=>"8", "year"=>"2010", "figure"=>"12", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"24", "year"=>"2010", "pdf"=>"13", "unique-ip"=>"26", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"38", "unique-ip"=>"35", "pdf"=>"23", "year"=>"2010", "figure"=>"9", "scanned-summary"=>"0", "supp-data"=>"3"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"24", "year"=>"2010", "pdf"=>"20", "unique-ip"=>"25", "figure"=>"23", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"27", "unique-ip"=>"26", "pdf"=>"18", "year"=>"2010", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"26", "year"=>"2010", "pdf"=>"16", "unique-ip"=>"27", "figure"=>"6", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"17", "unique-ip"=>"17", "pdf"=>"10", "year"=>"2010", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"13", "year"=>"2010", "pdf"=>"15", "unique-ip"=>"17", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"21", "unique-ip"=>"26", "pdf"=>"17", "year"=>"2010", "figure"=>"6", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"30", "year"=>"2010", "pdf"=>"15", "unique-ip"=>"27", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"22", "unique-ip"=>"25", "pdf"=>"12", "year"=>"2010", "figure"=>"13", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"1", "abstract"=>"1", "full-text"=>"12", "year"=>"2010", "pdf"=>"14", "unique-ip"=>"16", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"14", "unique-ip"=>"19", "pdf"=>"9", "year"=>"2011", "figure"=>"6", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"9", "year"=>"2011", "pdf"=>"4", "unique-ip"=>"16", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"11", "unique-ip"=>"11", "pdf"=>"12", "year"=>"2011", "figure"=>"12", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"8", "year"=>"2011", "pdf"=>"7", "unique-ip"=>"16", "figure"=>"13", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"17", "unique-ip"=>"17", "pdf"=>"13", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"10", "year"=>"2011", "pdf"=>"6", "unique-ip"=>"15", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"19", "unique-ip"=>"18", "pdf"=>"12", "year"=>"2011", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"24", "year"=>"2011", "pdf"=>"8", "unique-ip"=>"24", "figure"=>"11", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"19", "unique-ip"=>"17", "pdf"=>"9", "year"=>"2011", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"22", "year"=>"2011", "pdf"=>"9", "unique-ip"=>"22", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"1", "abstract"=>"2", "full-text"=>"15", "unique-ip"=>"14", "pdf"=>"4", "year"=>"2011", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"18", "year"=>"2011", "pdf"=>"6", "unique-ip"=>"19", "figure"=>"11", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"23", "unique-ip"=>"18", "pdf"=>"11", "year"=>"2012", "figure"=>"16", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"17", "year"=>"2012", "pdf"=>"10", "unique-ip"=>"15", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"13", "unique-ip"=>"16", "pdf"=>"5", "year"=>"2012", "figure"=>"6", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"1", "abstract"=>"0", "full-text"=>"11", "year"=>"2012", "pdf"=>"5", "unique-ip"=>"13", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"17", "unique-ip"=>"16", "pdf"=>"12", "year"=>"2012", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"3", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"105", "year"=>"2009", "pdf"=>"63", "unique-ip"=>"90", "figure"=>"31", "scanned-summary"=>"0", "supp-data"=>"15"}
  • {"scanned-page-browse"=>"0", "month"=>"4", "cited-by"=>"0", "abstract"=>"6", "full-text"=>"169", "unique-ip"=>"116", "pdf"=>"94", "year"=>"2009", "figure"=>"44", "scanned-summary"=>"0", "supp-data"=>"23"}
  • {"month"=>"5", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"110", "year"=>"2009", "pdf"=>"72", "unique-ip"=>"105", "figure"=>"27", "scanned-summary"=>"0", "supp-data"=>"10"}
  • {"scanned-page-browse"=>"0", "month"=>"6", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"90", "unique-ip"=>"78", "pdf"=>"68", "year"=>"2009", "figure"=>"42", "scanned-summary"=>"0", "supp-data"=>"10"}
  • {"month"=>"7", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"61", "year"=>"2009", "pdf"=>"54", "unique-ip"=>"58", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"18"}
  • {"scanned-page-browse"=>"0", "month"=>"8", "cited-by"=>"0", "abstract"=>"8", "full-text"=>"53", "unique-ip"=>"56", "pdf"=>"34", "year"=>"2009", "figure"=>"12", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"9", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"51", "year"=>"2009", "pdf"=>"34", "unique-ip"=>"43", "figure"=>"9", "scanned-summary"=>"0", "supp-data"=>"4"}
  • {"scanned-page-browse"=>"0", "month"=>"10", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"26", "unique-ip"=>"25", "pdf"=>"16", "year"=>"2009", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"month"=>"11", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"32", "year"=>"2009", "pdf"=>"7", "unique-ip"=>"35", "figure"=>"11", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"scanned-page-browse"=>"0", "month"=>"12", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"27", "unique-ip"=>"25", "pdf"=>"4", "year"=>"2009", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"13", "year"=>"2012", "pdf"=>"12", "unique-ip"=>"15", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"15", "full-text"=>"11", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"15", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"12", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"18", "full-text"=>"20", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"3", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"27", "full-text"=>"34", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"21", "full-text"=>"20", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"15", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"20", "full-text"=>"24", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"19", "full-text"=>"18", "pdf"=>"8", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"16", "full-text"=>"19", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"37", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"19", "full-text"=>"23", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"17", "full-text"=>"19", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"22", "full-text"=>"12", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"7", "cited-by"=>"1", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"18", "full-text"=>"10", "pdf"=>"14", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"3", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"29", "full-text"=>"15", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"5", "cited-by"=>"1", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"28", "full-text"=>"24", "pdf"=>"10", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"1", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"22", "full-text"=>"17", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}

Relative Metric

{"start_date"=>"2009-01-01T00:00:00Z", "end_date"=>"2009-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Medicine and health sciences", "average_usage"=>[332, 584, 719, 824, 927, 1030, 1139, 1233, 1322, 1402, 1483, 1570, 1649, 1730, 1806, 1873, 1939, 2003, 2068, 2130, 2191, 2248, 2302, 2362, 2427, 2485, 2544, 2599, 2653, 2709, 2778, 2840, 2896, 2950, 3013, 3073, 3137, 3189, 3253, 3305, 3364, 3423, 3489, 3557, 3606, 3668, 3742, 3797, 3852, 3907, 3962, 4027, 4072, 4132, 4193, 4256, 4308, 4366, 4427, 4493, 4556]}, {"subject_area"=>"/Medicine and health sciences/Pharmacology", "average_usage"=>[342, 657, 817, 960, 1071, 1171, 1305, 1411, 1508, 1596, 1709, 1785, 1855, 1945, 2032, 2127, 2192, 2260, 2343, 2398, 2455, 2506, 2557, 2629, 2698, 2774, 2864, 2945, 2992, 3069, 3128, 3181, 3244, 3310, 3371, 3441, 3494, 3565, 3647, 3706, 3760, 3841, 3924, 3977, 4046, 4095, 4159, 4314, 4376, 4455, 4517, 4587, 4674, 4737, 4817, 4868, 4914, 5001, 5064, 5119, 5170, 5210]}, {"subject_area"=>"/Medicine and health sciences/Tropical diseases", "average_usage"=>[262, 539, 658, 749, 834, 916, 1020, 1108, 1196, 1250, 1338, 1408, 1481, 1549, 1611, 1671, 1721, 1786, 1854, 1904, 1968, 2031, 2101, 2158, 2205, 2271, 2326, 2381, 2440, 2488, 2544, 2603, 2658, 2726, 2786, 2839, 2896, 2954, 3019, 3067, 3126, 3181, 3241, 3294, 3367, 3446, 3538, 3634, 3677, 3736, 3785, 3816, 3867, 3946, 4032, 4083, 4121, 4191, 4278, 4344, 4399, 4438]}]}
Loading … Spinner
There are currently no alerts