Analysis of Blood Stem Cell Activity and Cystatin Gene Expression in a Mouse Model Presenting a Chromosomal Deletion Encompassing Csta and Stfa2l1
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{"title"=>"Analysis of blood stem cell activity and cystatin gene expression in a mouse model presenting a chromosomal deletion encompassing Csta and Stfa2l1", "type"=>"journal", "authors"=>[{"first_name"=>"Mélanie", "last_name"=>"Bilodeau", "scopus_author_id"=>"14818892600"}, {"first_name"=>"Tara", "last_name"=>"MacRae", "scopus_author_id"=>"35264806700"}, {"first_name"=>"Louis", "last_name"=>"Gaboury", "scopus_author_id"=>"6701877555"}, {"first_name"=>"Jean Philippe", "last_name"=>"Laverdure", "scopus_author_id"=>"26432080400"}, {"first_name"=>"Marie Pierre", "last_name"=>"Hardy", "scopus_author_id"=>"8931020200"}, {"first_name"=>"Nadine", "last_name"=>"Mayotte", "scopus_author_id"=>"7801570710"}, {"first_name"=>"Véronique", "last_name"=>"Paradis", "scopus_author_id"=>"8642440500"}, {"first_name"=>"Sébastien", "last_name"=>"Harton", "scopus_author_id"=>"35263945200"}, {"first_name"=>"Claude", "last_name"=>"Perreault", "scopus_author_id"=>"7005537795"}, {"first_name"=>"Guy", "last_name"=>"Sauvageau", "scopus_author_id"=>"7003322258"}], "year"=>2009, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-70449372852", "sgr"=>"70449372852", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0007500", "pmid"=>"19838297", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pui"=>"355609496"}, "id"=>"07212b0d-6f77-34e3-827d-4907d269fb2e", "abstract"=>"The cystatin protein superfamily is characterized by the presence of conserved sequences that display cysteine protease inhibitory activity (e.g., towards cathepsins). Type 1 and 2 cystatins are encoded by 25 genes of which 23 are grouped in 2 clusters localized on mouse chromosomes 16 and 2. The expression and essential roles of most of these genes in mouse development and hematopoiesis remain poorly characterized. In this study, we describe a set of quantitative real-time PCR assays and a global expression profile of cystatin genes in normal mouse tissues. Benefiting from our collection of DelES embryonic stem cell clones harboring large chromosomal deletions (to be reported elsewhere), we selected a clone in which a 95-kb region of chromosome 16 is missing (Del(16qB3Delta/+)). In this particular clone, 2 cystatin genes, namely Csta and Stfa2l1 are absent along with 2 other genes (Fam162a, Ccdc58) and associated intergenic regions. From this line, we established a new homozygous mutant mouse model (Del(16qB3Delta/16qB3Delta)) to assess the in vivo biological functions of the 2 deleted cystatins. Stfa2l1 gene expression is high in wild-type fetal liver, bone marrow, and spleen, while Csta is ubiquitously expressed. Homozygous Del(16qB3Delta/16qB3Delta) animals are phenotypically normal, fertile, and not overtly susceptible to spontaneous or irradiation-induced tumor formation. The hematopoietic stem and progenitor cell activity in these mutant mice are also normal. Interestingly, quantitative real-time PCR expression profiling reveals a marked increase in the expression levels of Stfa2l1/Csta phylogenetically-related genes (Stfa1, Stfa2, and Stfa3) in Del(16qB3Delta/16qB3Delta) hematopoietic tissues, suggesting that these candidate genes might be contributing to compensatory mechanisms. Overall, this study presents an optimized approach to globally monitor cystatin gene expression as well as a new mouse model deficient in Stfa2l1/Csta genes, expanding the available tools to dissect cystatin roles under normal and pathological conditions.", "link"=>"http://www.mendeley.com/research/analysis-blood-stem-cell-activity-cystatin-gene-expression-mouse-model-presenting-chromosomal-deleti", "reader_count"=>20, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Student > Doctoral Student"=>1, "Researcher"=>2, "Student > Ph. D. Student"=>6, "Other"=>5, "Student > Master"=>1, "Student > Bachelor"=>1, "Professor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Student > Doctoral Student"=>1, "Researcher"=>2, "Student > Ph. D. Student"=>6, "Other"=>5, "Student > Master"=>1, "Student > Bachelor"=>1, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>11, "Medicine and Dentistry"=>3, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Physics and Astronomy"=>1, "Social Sciences"=>1, "Computer Science"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Social Sciences"=>{"Social Sciences"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>11}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/879734"], "description"=>"<p>M, male; F, female; +/+, wild-type; +/−, heterozygous <i>Del<sup>16qB3Δ/+</sup></i>; −/−, homozygous <i>Del<sup>16qB3Δ/16qB3Δ</sup></i> ; occ, occasional.</p>", "links"=>[], "tags"=>["hematological", "studies", "wild-type", "mutant", "littermate"], "article_id"=>550176, "categories"=>["Genetics", "Computational Biology"], "users"=>["Mélanie Bilodeau", "Tara MacRae", "Louis Gaboury", "Jean-Philippe Laverdure", "Marie-Pierre Hardy", "Nadine Mayotte", "Véronique Paradis", "Sébastien Harton", "Claude Perreault", "Guy Sauvageau"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0007500.t003", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Clinical_hematological_studies_of_wild_type_and_mutant_littermate_animals_/550176", "title"=>"Clinical hematological studies of wild-type and mutant littermate animals.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2009-10-19 00:02:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/879642"], "description"=>"<p>*Gene deleted in <i>Del<sup>16qB3Δ/+</sup></i> ESCs. Chr, chromosomal localization.</p>", "links"=>[], "tags"=>["cystatin", "chromosomal", "corresponding"], "article_id"=>550085, "categories"=>["Genetics", "Computational Biology"], "users"=>["Mélanie Bilodeau", "Tara MacRae", "Louis Gaboury", "Jean-Philippe Laverdure", "Marie-Pierre Hardy", "Nadine Mayotte", "Véronique Paradis", "Sébastien Harton", "Claude Perreault", "Guy Sauvageau"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0007500.t001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_List_of_type_1_amp_2_cystatin_genes_chromosomal_localization_and_corresponding_protein_names_/550085", "title"=>"List of type 1 & 2 cystatin genes, chromosomal localization, and corresponding protein names.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2009-10-19 00:01:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/879776"], "description"=>"a<p>Cellular populations were gated to exclude debris, dead cells and enucleated erythrocytes. Two mice of each genotype were analyzed (one male and one female).</p>b<p>Depletion of erythroid (Ter119), granulocytes (Gr1), and B cell (B220) lineages.</p>c<p>Depletion of TCRαβ<sup>+</sup>, TCRγδ<sup>+</sup>, NK1-1<sup>+</sup>, CD8<sup>+</sup>, CD3<sup>+</sup>, B220<sup>+</sup>, CD11b<sup>+</sup>, CD11c<sup>+</sup>, Gr1<sup>+</sup> and Ter119<sup>+</sup> cells. ETP, early T-cell precursor; DN2 & DN3, double negative phase 2&3; Hi, high expression level; Lo, low expression level.</p>", "links"=>[], "tags"=>["hematopoietic", "progenitors", "differentiated", "cells", "thymus", "analysed"], "article_id"=>550219, "categories"=>["Genetics", "Computational Biology"], "users"=>["Mélanie Bilodeau", "Tara MacRae", "Louis Gaboury", "Jean-Philippe Laverdure", "Marie-Pierre Hardy", "Nadine Mayotte", "Véronique Paradis", "Sébastien Harton", "Claude Perreault", "Guy Sauvageau"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0007500.t004", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Proportion_of_hematopoietic_progenitors_and_differentiated_cells_in_bone_marrow_spleen_and_thymus_analysed_by_flow_cytometry_a_/550219", "title"=>"Proportion of hematopoietic progenitors and differentiated cells in bone marrow, spleen, and thymus analysed by flow cytometry<sup>a</sup>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2009-10-19 00:03:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/879155"], "description"=>"<p>(A) Representation of the 95-kb haploid chromosomal deletion found in an ESC clone (7–30) engineered by a retroviral-based Cre-<i>loxP</i> system <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007500#pone.0007500-Bilodeau1\" target=\"_blank\">[1]</a>. The schema was adapted from UCSC Genome Browser (<a href=\"http://genome.ucsc.edu/\" target=\"_blank\">http://genome.ucsc.edu/</a>) <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007500#pone.0007500-Kuhn1\" target=\"_blank\">[3]</a>. Mammal conservation (blue histogram) represents a evolutionary conservation measure across 20 placental mammal genomes. In addition, 8 pairwise alignments of vertebrate genomes to the mouse genome can be found underneath (greyscale density plots) <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007500#pone.0007500-Kuhn1\" target=\"_blank\">[3]</a>. Also indicated, the recombined provirus originating from the recombination of two independent proviruses (A1 and S1, see reference <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007500#pone.0007500-Bilodeau1\" target=\"_blank\">[1]</a> for details). The coupling of the <i>Pgk</i> (murine phosphoglycerate kinase promoter)-ATG in S1 to the neomycin (neo ATGless) gene in A1 allowed the selection of recombinant ESC clones. LTR, long terminal repeat; SIN, long terminal repeat containing a deletion in the U3 region; ▸, <i>LoxP</i> site. (B) PCR-mediated genotyping (top panel) performed with genomic DNA extracted from wild-type (+/+) ESCs, engineered ESCs (+/−), and mouse tail clips from littermate animals obtained by intercrossing heterozygous (+/−) mice. Southern blot analysis performed with a neomycin probe (bottom panel, also see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007500#pone-0007500-g001\" target=\"_blank\">Figure 1A</a> for <i>Eco</i>RI enzymatic restriction digest pattern and neomycin probe localization) confirming the presence of the engineered allele in heterozygous or homozygous (−/−) mutant animals. wt, wild-type allele; del, deleted allele. (C) PCR products spanning exons (ex) or intron-exon boundaries confirmed the loss of the mapped genomic region in a representative homozygous animal (see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007500#pone-0007500-g001\" target=\"_blank\">Figure 1A</a> for exon numbering and <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007500#pone.0007500.s002\" target=\"_blank\">Table S2</a> for PCR assay details). CTL, control. PCR for the <i>Hprt1</i> gene was performed as a positive control in duplex or in parallel. (D) Representative photo of adult wild-type and homozygous <i>Del<sup>16qB3Δ/16qB3Δ</sup></i> mice.</p>", "links"=>[], "tags"=>["mutant"], "article_id"=>549613, "categories"=>["Genetics", "Computational Biology"], "users"=>["Mélanie Bilodeau", "Tara MacRae", "Louis Gaboury", "Jean-Philippe Laverdure", "Marie-Pierre Hardy", "Nadine Mayotte", "Véronique Paradis", "Sébastien Harton", "Claude Perreault", "Guy Sauvageau"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0007500.g001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Generation_of_mutant_mice_/549613", "title"=>"Generation of mutant mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-10-19 02:40:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/879356"], "description"=>"<p>(A–C) Clonogenic hematopoietic progenitor assays. Distribution of the number of hematopoietic colonies derived from myeloid clonogenic progenitors isolated from the bone marrow (A, seeding density of 4×10<sup>4</sup> cells) or the spleen (B, seeding density of 5×10<sup>5</sup> cells) of n = 2 animals of each indicated genotype. GM, granulocyte-macrophage progenitor; GEMM, granulocyte-erythrocyte-macrophage-megakaryocyte progenitor; M, macrophage progenitor; G, granulocyte progenitor; BFU-E, erythroid blast-forming unit. (C) Number of hematopoietic colonies derived from B-lymphoid clonogenic progenitors isolated from the bone marrow (seeding density of 2×10<sup>5</sup> cells) or spleen (seeding density of 2×10<sup>6</sup> cells) of n = 2 animals of each indicated genotype. (D) Competitive hematopoietic reconstitution assay. Representative flow cytometry profiles of peripheral blood cells stained with antibodies specific to allelic forms of the cell surface marker CD45: CD45.1<sup>+</sup> Pep3b recipient mouse (top left profile), CD45.2<sup>+</sup> C57BL/6J control mouse (top right profile), and immunophenotyping performed 12 weeks following the transplantation (tx) of the indicated ratio of <i>Del<sup>16qB3Δ/16qB3Δ</sup></i> (CD45.2<sup>+</sup>) and wild-type (CD45.1<sup>+</sup>) fetal liver cells (bottom profile). <i>Del<sup>16qB3Δ/16qB3Δ</sup></i> fetal liver cells efficiently contributed to the hematopoietic reconstitution of the host (D, bottom profile, bottom right quadrant). Note that the small proportion of double positive CD45.1<sup>+</sup> CD45.2<sup>+</sup> cells (D, bottom profile, upper right quadrant) represents a technical artefact; hematopoietic cells in reconstituted animals are exclusively positive for one of the two CD45 allelic forms.</p>", "links"=>[], "tags"=>["genetics and genomics/animal genetics", "genetics and genomics/gene expression", "hematology/hematopoiesis"], "article_id"=>549808, "categories"=>["Genetics", "Computational Biology"], "users"=>["Mélanie Bilodeau", "Tara MacRae", "Louis Gaboury", "Jean-Philippe Laverdure", "Marie-Pierre Hardy", "Nadine Mayotte", "Véronique Paradis", "Sébastien Harton", "Claude Perreault", "Guy Sauvageau"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0007500.g003", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Hematopoietic_system_characterization_/549808", "title"=>"Hematopoietic system characterization.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-10-19 02:43:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/879683"], "description"=>"a<p>Clinical serum biochemistry and urinalysis were performed with different sets of representative animals. M, male; F, female; +/+, wild-type; +/−, heterozygous <i>Del<sup>16qB3Δ/+</sup></i>; −/−, homozygous <i>Del<sup>16qB3Δ/16qB3Δ</sup></i> ; n.d., not determined.</p>", "links"=>[], "tags"=>["serum", "Biochemistry", "urinalysis", "mutant", "mice"], "article_id"=>550130, "categories"=>["Genetics", "Computational Biology"], "users"=>["Mélanie Bilodeau", "Tara MacRae", "Louis Gaboury", "Jean-Philippe Laverdure", "Marie-Pierre Hardy", "Nadine Mayotte", "Véronique Paradis", "Sébastien Harton", "Claude Perreault", "Guy Sauvageau"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0007500.t002", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Clinical_serum_biochemistry_and_urinalysis_of_mutant_mice_and_control_littermates_a_/550130", "title"=>"Clinical serum biochemistry and urinalysis of mutant mice and control littermates<sup>a</sup>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2009-10-19 00:02:10"}
  • {"files"=>["https://ndownloader.figshare.com/files/435800", "https://ndownloader.figshare.com/files/435813", "https://ndownloader.figshare.com/files/435831", "https://ndownloader.figshare.com/files/435844", "https://ndownloader.figshare.com/files/435917"], "description"=>"<div><p>The cystatin protein superfamily is characterized by the presence of conserved sequences that display cysteine protease inhibitory activity (e.g., towards cathepsins). Type 1 and 2 cystatins are encoded by 25 genes of which 23 are grouped in 2 clusters localized on mouse chromosomes 16 and 2. The expression and essential roles of most of these genes in mouse development and hematopoiesis remain poorly characterized. In this study, we describe a set of quantitative real-time PCR assays and a global expression profile of cystatin genes in normal mouse tissues. Benefiting from our collection of DelES embryonic stem cell clones harboring large chromosomal deletions (to be reported elsewhere), we selected a clone in which a 95-kb region of chromosome 16 is missing (<em>Del<sup>16qB3Δ/+</sup></em>). In this particular clone, 2 cystatin genes, namely <em>Csta</em> and <em>Stfa2l1</em> are absent along with 2 other genes (<em>Fam162a</em>, <em>Ccdc58</em>) and associated intergenic regions. From this line, we established a new homozygous mutant mouse model (<em>Del<sup>16qB3Δ/16qB3Δ</sup></em>) to assess the <em>in vivo</em> biological functions of the 2 deleted cystatins. <em>Stfa2l1</em> gene expression is high in wild-type fetal liver, bone marrow, and spleen, while <em>Csta</em> is ubiquitously expressed. Homozygous <em>Del<sup>16qB3Δ/16qB3Δ</sup></em> animals are phenotypically normal, fertile, and not overtly susceptible to spontaneous or irradiation-induced tumor formation. The hematopoietic stem and progenitor cell activity in these mutant mice are also normal. Interestingly, quantitative real-time PCR expression profiling reveals a marked increase in the expression levels of <em>Stfa2l1/Csta</em> phylogenetically-related genes (<em>Stfa1</em>, <em>Stfa2</em>, and <em>Stfa3</em>) in <em>Del<sup>16qB3Δ/16qB3Δ</sup></em> hematopoietic tissues, suggesting that these candidate genes might be contributing to compensatory mechanisms. Overall, this study presents an optimized approach to globally monitor cystatin gene expression as well as a new mouse model deficient in <em>Stfa2l1/Csta</em> genes, expanding the available tools to dissect cystatin roles under normal and pathological conditions.</p></div>", "links"=>[], "tags"=>["cystatin", "presenting", "chromosomal", "deletion", "encompassing"], "article_id"=>145939, "categories"=>["Genetics", "Biological Sciences"], "users"=>["Mélanie Bilodeau", "Tara MacRae", "Louis Gaboury", "Jean-Philippe Laverdure", "Marie-Pierre Hardy", "Nadine Mayotte", "Véronique Paradis", "Sébastien Harton", "Claude Perreault", "Guy Sauvageau"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0007500.s001", "https://dx.doi.org/10.1371/journal.pone.0007500.s002", "https://dx.doi.org/10.1371/journal.pone.0007500.s003", "https://dx.doi.org/10.1371/journal.pone.0007500.s004", "https://dx.doi.org/10.1371/journal.pone.0007500.s005"], "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Analysis_of_Blood_Stem_Cell_Activity_and_Cystatin_Gene_Expression_in_a_Mouse_Model_Presenting_a_Chromosomal_Deletion_Encompassing_Csta_and_Stfa2l1_/145939", "title"=>"Analysis of Blood Stem Cell Activity and Cystatin Gene Expression in a Mouse Model Presenting a Chromosomal Deletion Encompassing <em>Csta</em> and <em>Stfa2l1</em>", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2009-10-19 01:38:59"}
  • {"files"=>["https://ndownloader.figshare.com/files/879459"], "description"=>"<p>(A–D) Relative gene expression monitored by qRT-PCR using RNA extracted from hematopoietic tissues of 2–4 independent mice for each indicated genotype (+/+, wild-type; +/−, heterozygous <i>Del<sup>16qB3Δ/+</sup></i>; and −/−, homozygous <i>Del<sup>16qB3Δ/16qB3Δ</sup></i>). Average expression level of each indicated gene in each wild-type tissue was used as a calibrator for normalization (see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007500#s2\" target=\"_blank\">Material and Methods</a> for details). Relative gene expression observed for each independent tissue is represented by a colored dot or a Δ when the result is below the detection level of the assay; relative gene expression average is represented by an horizontal bar. Loss of expression of <i>Fam162a</i>, <i>Ccdc58</i>, <i>Csta</i>, and <i>Stfa2l1</i> was confirmed in <i>Del<sup>16qB3Δ/16qB3Δ</sup></i> mouse tissues. Distinctive increases in transcriptional levels were observed for <i>Stfa1</i>, <i>Stfa2</i>, and <i>Stfa3</i> in tissues of heterozygous <i>Del<sup>16qB3Δ/+</sup></i> and homozygous <i>Del<sup>16qB3Δ/16qB3Δ</sup></i> adult mice compared to wild-type (B–D), but not in the fetal livers (A). Note that <i>Stfa1</i> qRT-PCR primers also detect <i>BC117090</i> and BC100530 gene expression. *, Genes deleted in <i>Del<sup>16qB3Δ/+</sup></i> ESCs.</p>", "links"=>[], "tags"=>["profiling", "wild-type", "mutant", "hematopoietic"], "article_id"=>549908, "categories"=>["Genetics", "Computational Biology"], "users"=>["Mélanie Bilodeau", "Tara MacRae", "Louis Gaboury", "Jean-Philippe Laverdure", "Marie-Pierre Hardy", "Nadine Mayotte", "Véronique Paradis", "Sébastien Harton", "Claude Perreault", "Guy Sauvageau"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0007500.g004", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparative_expression_profiling_of_wild_type_and_mutant_hematopoietic_tissues_/549908", "title"=>"Comparative expression profiling of wild-type and mutant hematopoietic tissues.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-10-19 02:45:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/879273"], "description"=>"<p>(A) Phylogeny inference of type 1 and 2 cystatins based on amino acids sequence comparison. Numbers represent the degree of confidence of the nodes, as evaluated by the bootstrap test (highest degree of confidence: 1000). *Genes deleted in <i>Del<sup>16qB3Δ/+</sup></i> ESCs. (B) ΔCt heatmap representing the expression profiles of <i>Fam162a</i>, <i>Ccdc58</i>, and several cystatin genes in the indicated tissues of adult mice (n = 2 independent tissues analyzed in duplicate, see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007500#s2\" target=\"_blank\">Material and Methods</a> section for details). Note that the qRT-PCR assay for <i>Stfa1</i> also detects <i>BC117090</i> and <i>BC100530</i> transcripts. ΔCt above 15 represents weak gene expression or no expression; the expression cut-off varies according to each specific qRT-PCR assay (between ΔCt 15–25).</p>", "links"=>[], "tags"=>["genetics and genomics/animal genetics", "genetics and genomics/gene expression", "hematology/hematopoiesis"], "article_id"=>549721, "categories"=>["Genetics", "Computational Biology"], "users"=>["Mélanie Bilodeau", "Tara MacRae", "Louis Gaboury", "Jean-Philippe Laverdure", "Marie-Pierre Hardy", "Nadine Mayotte", "Véronique Paradis", "Sébastien Harton", "Claude Perreault", "Guy Sauvageau"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0007500.g002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comprehensive_analysis_of_type_1_and_2_mouse_Cystatins_/549721", "title"=>"Comprehensive analysis of type 1 and 2 mouse Cystatins.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-10-19 02:42:01"}

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Relative Metric

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