TNF-α Regulates the Effects of Irradiation in the Mouse Bone Marrow Microenvironment
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"TNF-α regulates the effects of irradiation in the mouse bone marrow microenvironment", "type"=>"journal", "authors"=>[{"first_name"=>"Ana Sofia", "last_name"=>"Cachaço", "scopus_author_id"=>"18433326200"}, {"first_name"=>"Tânia", "last_name"=>"Carvalho", "scopus_author_id"=>"25653983600"}, {"first_name"=>"Ana Cristina", "last_name"=>"Santos", "scopus_author_id"=>"15737598300"}, {"first_name"=>"Cátia", "last_name"=>"Igreja", "scopus_author_id"=>"57195053404"}, {"first_name"=>"Rita", "last_name"=>"Fragoso", "scopus_author_id"=>"23034139300"}, {"first_name"=>"Catarina Osó", "last_name"=>"Rio", "scopus_author_id"=>"36027738100"}, {"first_name"=>"Manuela", "last_name"=>"Ferreira", "scopus_author_id"=>"7402787225"}, {"first_name"=>"Jacinta", "last_name"=>"Serpa", "scopus_author_id"=>"56623388500"}, {"first_name"=>"Sofia", "last_name"=>"Correia", "scopus_author_id"=>"24467217500"}, {"first_name"=>"Perpétua", "last_name"=>"Pinto-do-Ó", "scopus_author_id"=>"57199512557"}, {"first_name"=>"Sérgio", "last_name"=>"Dias", "scopus_author_id"=>"7007078291"}], "year"=>2010, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"20126546", "doi"=>"10.1371/journal.pone.0008980", "sgr"=>"77749280454", "scopus"=>"2-s2.0-77749280454", "issn"=>"19326203", "pui"=>"358425903"}, "id"=>"e24c5677-d71a-395c-9139-58bfd6267647", "abstract"=>"Background: Secondary bone marrow (BM) myelodysplastic syndromes (MDS) are increasingly common, as a result of radio or chemotherapy administered to a majority of cancer patients. Patients with secondary MDS have increased BM cell apoptosis, which results in BM dysfunction (cytopenias), and an increased risk of developing fatal acute leukemias. In the present study we asked whether TNF-α, known to regulate cell apoptosis, could modulate the onset of secondary MDS. Principal Findings: We show that TNF-α is induced by irradiation and regulates BM cells apoptosis in vitro and in vivo. In contrast to irradiated wild type (WT) mice, TNF-α deficient (TNF-α KO) mice or WT mice treated with a TNF-α-neutralizing antibody were partially protected from the apoptotic effects of irradiation. Next we established a 3-cycle irradiation protocol, in which mice were sub-lethally irradiated once monthly over a 3 month period. In this model, irradiated WT mice presented loss of microsatellite markers on BM cells, low white blood cell (WBC) counts, reduced megakaryocyte (MK) and platelet levels (thrombocytopenia) and macrocytic anemia, phenoypes that suggest the irradiation protocol resulted in BM dysfunction with clinical features of MDS. In contrast, TNF-α KO mice were protected from the irradiation effects: BM cell apoptosis following irradiation was significantly reduced, concomitant with sustained BM MK numbers and absence of other cytopenias. Moreover, irradiated WT mice with long term (≥5 months) BM dysfunction had increased BM angiogenesis, MMPs and VEGF and NFkB p65, suggestive of disease progression. Conclusion: Taken together, our data shows that TNF-α induction following irradiation modulates BM cell apoptosis and is a crucial event in BM dysfunction, secondary MDS onset and progression.", "link"=>"http://www.mendeley.com/research/tnf%CE%B1-regulates-effects-irradiation-mouse-bone-marrow-microenvironment", "reader_count"=>6, "reader_count_by_academic_status"=>{"Researcher"=>2, "Student > Ph. D. Student"=>1, "Other"=>2, "Student > Postgraduate"=>1}, "reader_count_by_user_role"=>{"Researcher"=>2, "Student > Ph. D. Student"=>1, "Other"=>2, "Student > Postgraduate"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>1, "Veterinary Science and Veterinary Medicine"=>1, "Medicine and Dentistry"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/77749280454"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/77749280454?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77749280454&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=77749280454&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/77749280454", "dc:identifier"=>"SCOPUS_ID:77749280454", "eid"=>"2-s2.0-77749280454", "dc:title"=>"TNF-α regulates the effects of irradiation in the mouse bone marrow microenvironment", "dc:creator"=>"Cachaço A.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"5", "prism:issueIdentifier"=>"2", "prism:pageRange"=>nil, "prism:coverDate"=>"2010-02-01", "prism:coverDisplayDate"=>"1 February 2010", "prism:doi"=>"10.1371/journal.pone.0008980", "citedby-count"=>"27", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Instituto Portugues de Oncologia de Francisco Gentil Lisboa", "affiliation-city"=>"Lisbon", "affiliation-country"=>"Portugal"}], "pubmed-id"=>"20126546", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e8980", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0008980", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"12", "year"=>"2009", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"1", "year"=>"2010", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"2", "year"=>"2010", "pdf_views"=>"43", "xml_views"=>"1", "html_views"=>"174"}
  • {"month"=>"3", "year"=>"2010", "pdf_views"=>"26", "xml_views"=>"1", "html_views"=>"80"}
  • {"month"=>"4", "year"=>"2010", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"5", "year"=>"2010", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"83"}
  • {"month"=>"6", "year"=>"2010", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"7", "year"=>"2010", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"8", "year"=>"2010", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"9", "year"=>"2010", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"62"}
  • {"month"=>"10", "year"=>"2010", "pdf_views"=>"18", "xml_views"=>"2", "html_views"=>"51"}
  • {"month"=>"11", "year"=>"2010", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"12", "year"=>"2010", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"1", "year"=>"2011", "pdf_views"=>"16", "xml_views"=>"2", "html_views"=>"50"}
  • {"month"=>"2", "year"=>"2011", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"3", "year"=>"2011", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"4", "year"=>"2011", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"5", "year"=>"2011", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"37"}
  • {"month"=>"6", "year"=>"2011", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"7", "year"=>"2011", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"8", "year"=>"2011", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"9", "year"=>"2011", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"10", "year"=>"2011", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"49"}
  • {"month"=>"11", "year"=>"2011", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"42"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"52"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"10", "xml_views"=>"5", "html_views"=>"24"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"51"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"61"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"4", "html_views"=>"23"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"37"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"3", "html_views"=>"67"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"48"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"60"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"28"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"62"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"3", "html_views"=>"71"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"69"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"89"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"71"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"74"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"3", "html_views"=>"6"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"9"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/865519"], "description"=>"<p><b>A.</b> BM cryosections of WT and TNF-α KO mice immunostained for CD-41, a megakaryocyte marker, shows increased megakaryocytes in TNF-α KO BM. Nuclei are stained in blue with Dapi. Scale bar  = 50 µM. <b>B.</b> Flow cytometry for CD41 in BM samples corroborates the idea that in the absence of TNF-α the proportion of MK is maintained or even shows a slight increase following irradiation. These results were obtained from 3 independent experiments, using 3 animals per experimental group. *: p<0.05.</p>", "links"=>[], "tags"=>["bm", "mk", "wt", "ko"], "article_id"=>535968, "categories"=>["Cancer", "Medicine"], "users"=>["Ana Sofia Cachaço", "Tânia Carvalho", "Ana Cristina Santos", "Cátia Igreja", "Rita Fragoso", "Catarina Osório", "Manuela Ferreira", "Jacinta Serpa", "Sofia Correia", "Perpétua Pinto-do-Ó", "Sergio Dias"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0008980.g006", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Irradiation_reduces_the_BM_MK_content_in_WT_but_not_TNF_945_KO_mice_/535968", "title"=>"Irradiation reduces the BM MK content in WT but not TNF-α KO mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-02-01 01:39:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/865644"], "description"=>"<p><b>A.</b> BM criosections of WT and KO mice immunostained for laminin. Laminin staining (green; nuclei stained in blue) which marks basement membranes shows an increase in microvessel density (more and more dilated vessels) in 3x irradiated WT mice BM (<b>B</b>) compared to control (non-irradiated) WT mice and TNF-α KO. Scale bar  = 50 µm. <b>B.</b> The expression of other ECM proteins also differs between WT mice BM and TNF-α KO mice BM. As determined by RQ-PCR, the expression of laminin and collagen IV is significantly reduced in TNF-α KO mice BM. These results were obtained from 3 independent experiments. *: p<0.05 for laminin and collagen IV.</p>", "links"=>[], "tags"=>["induces", "bm", "ecm", "turnover", "wt", "ko"], "article_id"=>536096, "categories"=>["Cancer", "Medicine"], "users"=>["Ana Sofia Cachaço", "Tânia Carvalho", "Ana Cristina Santos", "Cátia Igreja", "Rita Fragoso", "Catarina Osório", "Manuela Ferreira", "Jacinta Serpa", "Sofia Correia", "Perpétua Pinto-do-Ó", "Sergio Dias"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0008980.g007", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Irradiation_induces_BM_ECM_turnover_in_WT_but_not_TNF_945_KO_mice_/536096", "title"=>"Irradiation induces BM ECM turnover in WT but not TNF-α KO mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-02-01 01:41:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/864997"], "description"=>"<p><b>A.</b> TNF-α protein measured by ELISA indicates an increase in TNF-α levels after irradiation, both in supernatants of cultured total BM mononuclear cells and stromal cells. <b>B.</b> Apoptosis of BM cells, incubated with the supernatants obtained in A, untreated or treated with an anti-TNF-α antibody. The presence of TNF-α antibody significantly decreases cell apoptosis after irradiation, both for Sca1+ and CD11b+ cells. The results shown were obtained from 2 independent experiments. *: p<0.05 for CD11b+ and for Sca1+.</p>", "links"=>[], "tags"=>["bm", "apoptosis"], "article_id"=>535450, "categories"=>["Cancer", "Medicine"], "users"=>["Ana Sofia Cachaço", "Tânia Carvalho", "Ana Cristina Santos", "Cátia Igreja", "Rita Fragoso", "Catarina Osório", "Manuela Ferreira", "Jacinta Serpa", "Sofia Correia", "Perpétua Pinto-do-Ó", "Sergio Dias"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0008980.g002", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Irradiation_induced_TNF_release_results_in_BM_cell_apoptosis_in_vitro_/535450", "title"=>"Irradiation-induced TNF-α release results in BM cell apoptosis <i>in vitro</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-02-01 01:30:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/430173"], "description"=>"<div><h3>Background</h3><p>Secondary bone marrow (BM) myelodysplastic syndromes (MDS) are increasingly common, as a result of radio or chemotherapy administered to a majority of cancer patients. Patients with secondary MDS have increased BM cell apoptosis, which results in BM dysfunction (cytopenias), and an increased risk of developing fatal acute leukemias. In the present study we asked whether TNF-α, known to regulate cell apoptosis, could modulate the onset of secondary MDS.</p><h3>Principal Findings</h3><p>We show that TNF-α is induced by irradiation and regulates BM cells apoptosis <em>in vitro</em> and <em>in vivo</em>. In contrast to irradiated wild type (WT) mice, TNF-α deficient (TNF-α KO) mice or WT mice treated with a TNF-α-neutralizing antibody were partially protected from the apoptotic effects of irradiation. Next we established a 3-cycle irradiation protocol, in which mice were sub-lethally irradiated once monthly over a 3 month period. In this model, irradiated WT mice presented loss of microsatellite markers on BM cells, low white blood cell (WBC) counts, reduced megakaryocyte (MK) and platelet levels (thrombocytopenia) and macrocytic anemia, phenoypes that suggest the irradiation protocol resulted in BM dysfunction with clinical features of MDS. In contrast, TNF-α KO mice were protected from the irradiation effects: BM cell apoptosis following irradiation was significantly reduced, concomitant with sustained BM MK numbers and absence of other cytopenias. Moreover, irradiated WT mice with long term (≥5 months) BM dysfunction had increased BM angiogenesis, MMPs and VEGF and NFkB p65, suggestive of disease progression.</p><h3>Conclusion</h3><p>Taken together, our data shows that TNF-α induction following irradiation modulates BM cell apoptosis and is a crucial event in BM dysfunction, secondary MDS onset and progression.</p></div>", "links"=>[], "tags"=>["regulates", "effects", "irradiation", "marrow", "microenvironment"], "article_id"=>144911, "categories"=>["Cancer", "Medicine"], "users"=>["Ana Sofia Cachaço", "Tânia Carvalho", "Ana Cristina Santos", "Cátia Igreja", "Rita Fragoso", "Catarina Osório", "Manuela Ferreira", "Jacinta Serpa", "Sofia Correia", "Perpétua Pinto-do-Ó", "Sergio Dias"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0008980", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/TNF_Regulates_the_Effects_of_Irradiation_in_the_Mouse_Bone_Marrow_Microenvironment/144911", "title"=>"TNF-α Regulates the Effects of Irradiation in the Mouse Bone Marrow Microenvironment", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-02-01 01:21:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/864860"], "description"=>"<p><b>A.</b> Flow cytometry analysis of WT mouse BM cells shows a rapid increase in CD11b+ and Sca1+ cells apoptosis 6-12 hours after sub-lethal irradiation, returning to normal levels after 72 hrs. <b>B.</b> TNF-α quantification by RQ-PCR on the same samples indicates a rapid increase in TNF-α mRNA after irradiation. The results shown were obtained from 8 independent experiments using 6 animals per time point.</p>", "links"=>[], "tags"=>["induces", "bm", "apoptosis", "correlates"], "article_id"=>535310, "categories"=>["Cancer", "Medicine"], "users"=>["Ana Sofia Cachaço", "Tânia Carvalho", "Ana Cristina Santos", "Cátia Igreja", "Rita Fragoso", "Catarina Osório", "Manuela Ferreira", "Jacinta Serpa", "Sofia Correia", "Perpétua Pinto-do-Ó", "Sergio Dias"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0008980.g001", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Irradiation_induces_BM_cell_apoptosis_which_correlates_with_an_increase_in_TNF_945_expression_/535310", "title"=>"Irradiation induces BM cell apoptosis which correlates with an increase in TNFα expression.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-02-01 01:28:30"}
  • {"files"=>["https://ndownloader.figshare.com/files/865407"], "description"=>"<p><b>A.</b> TUNEL assay on BM cryosections shows increased apoptosis in 3x irradiated WT mice compared to control and to 3x irradiated TNF-α KO mice. Scale bar  = 50 µm. <b>B.</b> BM apoptotic index shows increased apoptosis in 3x irradiated WT mice. These results were obtained from 2 independent experiments, using 3 animals per experimental group. *: p<0.05.</p>", "links"=>[], "tags"=>["ko", "mice", "resistant", "irradiation-induced", "bm"], "article_id"=>535851, "categories"=>["Cancer", "Medicine"], "users"=>["Ana Sofia Cachaço", "Tânia Carvalho", "Ana Cristina Santos", "Cátia Igreja", "Rita Fragoso", "Catarina Osório", "Manuela Ferreira", "Jacinta Serpa", "Sofia Correia", "Perpétua Pinto-do-Ó", "Sergio Dias"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0008980.g005", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_TNF_945_KO_mice_are_resistant_to_irradiation_induced_BM_cell_apoptosis_/535851", "title"=>"TNF-α KO mice are resistant to irradiation-induced BM cell apoptosis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-02-01 01:37:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/865789"], "description"=>"<p><b>A.</b> Whole BM extracts obtained from control or irradiated WT and TNF-α KO mice were analysed by zymography. As shown by the classical MMP pattern seen in zymograms, MMP-9, MMP-2 and MMP-7 could be detected in BM extracts from all the mice. However, in irradiated WT mice (with prolonged MDS) the levels of MMP-9, MMP-2 and active MMP-2 and MMP-7 increased significantly. These results are representative of 3 mice per experimental group. <b>B.</b> Whole BM extracts were obtained from control or irradiated WT and TNF-α KO mice and analysed by western blotting. The results show a dramatic increase in NFkB p65 and in VEGF levels in BM extracts from irradiated WT mice with prolonged MDS, while in BM extracts from TNF-α deficient mice these parameters remain unchanged. These results are representative of 3 mice per experimental group.</p>", "links"=>[], "tags"=>["wt", "mice", "mmp", "activation", "nfkb", "vegf", "bm", "levels"], "article_id"=>536234, "categories"=>["Cancer", "Medicine"], "users"=>["Ana Sofia Cachaço", "Tânia Carvalho", "Ana Cristina Santos", "Cátia Igreja", "Rita Fragoso", "Catarina Osório", "Manuela Ferreira", "Jacinta Serpa", "Sofia Correia", "Perpétua Pinto-do-Ó", "Sergio Dias"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0008980.g008", "stats"=>{"downloads"=>1, "page_views"=>25, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_3xirradiated_WT_mice_show_MMP_activation_and_increased_NFkB_and_VEGF_BM_levels_in_BM_extracts_/536234", "title"=>"3xirradiated WT mice show MMP activation and increased NFkB and VEGF BM levels in BM extracts.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-02-01 01:43:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/865261"], "description"=>"<p><b>A</b>. The WBC is significantly reduced in 3x irradiated mice. <b>B.</b> The number of platelets is reduced in 3x irradiated mice. <b>C.</b> The number of RBC is significantly reduced in 3x irradiated mice. *: p<0.05. <b>D</b>. 3x irradiated mice present significantly higher MCH-pg Hemoglobin per RBC than control mice. *: P<0.05.</p>", "links"=>[], "tags"=>["3-cycle", "irradiation", "induces", "cytopenias", "macrocytic", "anemia", "wt"], "article_id"=>535703, "categories"=>["Cancer", "Medicine"], "users"=>["Ana Sofia Cachaço", "Tânia Carvalho", "Ana Cristina Santos", "Cátia Igreja", "Rita Fragoso", "Catarina Osório", "Manuela Ferreira", "Jacinta Serpa", "Sofia Correia", "Perpétua Pinto-do-Ó", "Sergio Dias"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0008980.g004", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_A_3_cycle_irradiation_protocol_induces_cytopenias_and_macrocytic_anemia_in_WT_mice_/535703", "title"=>"A 3-cycle irradiation protocol induces cytopenias and macrocytic anemia in WT mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-02-01 01:35:03"}
  • {"files"=>["https://ndownloader.figshare.com/files/865121"], "description"=>"<p><b>A.</b> WT and TNF-α KO mice were sub-lethally irradiated and the total number of BM cells was counted as explained in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0008980#s4\" target=\"_blank\">Methods</a>. As shown, TNF-α KO mice were more resistant to the apoptotic effects of irradiation. On day 3 following irradiation, the number of total BM cells is significantly higher in TNF-α KO mice than in WT mice. The results shown were obtained from two independent experiments, using 12 animals per experimental group and 3 animals per time point. *: p<0.05. <b>B.</b> WT mice were injected with PBS (control) or with antibody anti-TNFα prior to sub-lethal irradiation and total BM cells were counted. Like for TNF-α KO mice, anti-TNFα neutralized mice were more resistent to irradiation; here, the number of total BM cells is significantly higher (*: p<0.02) by 24 hours after irradiation than in controls. <b>C.</b> The percentage of apoptotic cells 24 hours after irradiation was obtained in control and neutralized mice by flow cytometry. Both precursor (Sca1+) and myeloid (CD11b+) cells were protected from irradiation-induced apoptosis in the anti-TNF-α treated mice, where the number of cells positive for annexin V is lower than in the controls. *: p<0.01 for CD11b+; for Sca1+ a p value could not be calculated due to the absence of Sca1+Annexin+ cells in neutralized mice. The results shown in B and C were obtained from one experiment, using 12 animals per experimental group and 3 animals per time-point.</p>", "links"=>[], "tags"=>["irradiation", "bm"], "article_id"=>535571, "categories"=>["Cancer", "Medicine"], "users"=>["Ana Sofia Cachaço", "Tânia Carvalho", "Ana Cristina Santos", "Cátia Igreja", "Rita Fragoso", "Catarina Osório", "Manuela Ferreira", "Jacinta Serpa", "Sofia Correia", "Perpétua Pinto-do-Ó", "Sergio Dias"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0008980.g003", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sub_lethal_irradiation_reduces_the_number_of_total_BM_cells_an_effect_partially_dependent_on_TNF_945_/535571", "title"=>"Sub-lethal irradiation reduces the number of total BM cells, an effect partially dependent on TNF-α.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-02-01 01:32:51"}

PMC Usage Stats | Further Information

  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"52", "year"=>"2010", "pdf"=>"35", "unique-ip"=>"57", "figure"=>"12", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"52", "unique-ip"=>"44", "pdf"=>"13", "year"=>"2010", "figure"=>"9", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"37", "year"=>"2010", "pdf"=>"23", "unique-ip"=>"40", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"46", "unique-ip"=>"49", "pdf"=>"23", "year"=>"2010", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"17", "year"=>"2010", "pdf"=>"16", "unique-ip"=>"19", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"18", "unique-ip"=>"24", "pdf"=>"13", "year"=>"2010", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"20", "year"=>"2010", "pdf"=>"11", "unique-ip"=>"24", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"22", "unique-ip"=>"24", "pdf"=>"8", "year"=>"2010", "figure"=>"14", "scanned-summary"=>"0", "supp-data"=>"3"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"27", "year"=>"2010", "pdf"=>"13", "unique-ip"=>"24", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"25", "unique-ip"=>"29", "pdf"=>"16", "year"=>"2010", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"19", "year"=>"2010", "pdf"=>"12", "unique-ip"=>"20", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"28", "unique-ip"=>"40", "pdf"=>"13", "year"=>"2011", "figure"=>"20", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"28", "year"=>"2011", "pdf"=>"20", "unique-ip"=>"37", "figure"=>"12", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"27", "unique-ip"=>"34", "pdf"=>"9", "year"=>"2011", "figure"=>"13", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"5", "full-text"=>"13", "year"=>"2011", "pdf"=>"8", "unique-ip"=>"24", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"14", "unique-ip"=>"20", "pdf"=>"9", "year"=>"2011", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"23", "year"=>"2011", "pdf"=>"13", "unique-ip"=>"25", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"11", "unique-ip"=>"13", "pdf"=>"8", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"21", "year"=>"2011", "pdf"=>"10", "unique-ip"=>"22", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"8", "unique-ip"=>"8", "pdf"=>"4", "year"=>"2011", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"16", "year"=>"2011", "pdf"=>"5", "unique-ip"=>"17", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"15", "unique-ip"=>"19", "pdf"=>"14", "year"=>"2011", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"14", "year"=>"2011", "pdf"=>"8", "unique-ip"=>"17", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"21", "unique-ip"=>"20", "pdf"=>"16", "year"=>"2012", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"16", "year"=>"2012", "pdf"=>"7", "unique-ip"=>"14", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"17", "unique-ip"=>"15", "pdf"=>"9", "year"=>"2012", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"1", "abstract"=>"0", "full-text"=>"14", "year"=>"2012", "pdf"=>"5", "unique-ip"=>"12", "figure"=>"14", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"11", "unique-ip"=>"11", "pdf"=>"5", "year"=>"2012", "figure"=>"10", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"12", "year"=>"2012", "pdf"=>"6", "unique-ip"=>"17", "figure"=>"12", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"14", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"15", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"32", "full-text"=>"20", "pdf"=>"10", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"7", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"3", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"5", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"9", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"7", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"17", "full-text"=>"18", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"21", "full-text"=>"14", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"18", "full-text"=>"18", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2010-01-01T00:00:00Z", "end_date"=>"2010-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Anatomy and physiology", "average_usage"=>[276, 544, 702, 826, 936, 1045, 1140, 1222, 1305, 1377, 1453, 1528, 1584, 1661, 1725, 1783, 1848, 1913, 1977, 2038, 2103, 2158, 2223, 2290, 2346, 2406, 2467, 2529, 2588, 2652, 2722, 2779, 2835, 2892, 2943, 2999]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[280, 562, 725, 863, 974, 1083, 1177, 1268, 1347, 1421, 1489, 1570, 1638, 1706, 1763, 1823, 1890, 1951, 2016, 2076, 2134, 2192, 2257, 2319, 2378, 2438, 2501, 2572, 2634, 2700, 2759, 2825, 2887, 2936, 3007, 3070, 3121, 3184, 3237, 3304, 3363, 3425, 3484, 3531, 3612, 3663, 3718, 3771]}, {"subject_area"=>"/Medicine and health sciences/Anatomy", "average_usage"=>[268, 527, 681, 804, 923, 1031, 1126, 1210, 1284, 1361, 1440, 1508, 1578, 1631, 1688, 1754, 1816, 1877, 1945, 2003, 2060, 2125, 2183, 2253, 2313, 2379, 2436, 2513, 2570, 2626, 2702, 2757, 2809, 2885, 2945, 3006, 3067, 3128, 3180, 3237, 3290, 3347, 3397, 3462, 3520, 3585, 3651, 3706, 3755]}, {"subject_area"=>"/Medicine and health sciences/Hematology", "average_usage"=>[241, 488, 623, 737, 840, 951, 1047, 1112, 1189, 1261, 1341, 1404, 1474, 1528, 1595, 1658, 1712, 1771, 1829, 1889, 1947, 2000, 2062, 2129, 2201, 2253, 2310, 2364, 2426, 2486, 2561, 2600, 2653, 2711, 2768, 2816, 2876, 2938, 2989, 3047, 3103, 3158, 3193, 3272, 3307, 3368, 3454, 3512, 3566]}, {"subject_area"=>"/Medicine and health sciences/Physiology", "average_usage"=>[279, 551, 699, 817, 930, 1027, 1124, 1209, 1287, 1362, 1438, 1512, 1582, 1650, 1717, 1772, 1831, 1888, 1948, 2002, 2070, 2138, 2203, 2267, 2331, 2395, 2467, 2523, 2592, 2656, 2718, 2785, 2839, 2892, 2960, 3034, 3087, 3144, 3194, 3254, 3313, 3373, 3446, 3496, 3551, 3607, 3660, 3712, 3773]}]}
Loading … Spinner
There are currently no alerts