T- and B-Cell-Mediated Protection Induced by Novel, Live Attenuated Pertussis Vaccine in Mice. Cross Protection against Parapertussis
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{"title"=>"T- and B-cell-mediated protection induced by novel, live attenuated pertussis vaccine in mice. Cross protection against parapertussis", "type"=>"journal", "authors"=>[{"first_name"=>"Pascal Feunou", "last_name"=>"Feunou", "scopus_author_id"=>"6507442719"}, {"first_name"=>"Julie", "last_name"=>"Bertout", "scopus_author_id"=>"24449241500"}, {"first_name"=>"Camille", "last_name"=>"Locht", "scopus_author_id"=>"7006712654"}], "year"=>2010, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "pmid"=>"20419113", "pui"=>"359482164", "scopus"=>"2-s2.0-77956307858", "doi"=>"10.1371/journal.pone.0010178", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "sgr"=>"77956307858"}, "id"=>"1daa6387-0bab-336b-b830-da13b94ddd4e", "abstract"=>"BACKGROUND Despite the extensive use of efficacious vaccines, pertussis still ranks among the major causes of childhood mortality worldwide. Two types of pertussis vaccines are currently available, whole-cell, and the more recent acellular vaccines. Because of reduced reactogenicity and comparable efficacy acellular vaccines progressively replace whole-cell vaccines. However, both types require repeated administrations for optimal efficacy. We have recently developed a live attenuated vaccine candidate, named BPZE1, able to protect infant mice after a single nasal administration. METHODOLOGY/PRINCIPAL FINDINGS We determined the protective mechanism of BPZE1-mediated immunity by using passive transfer of T cells and antibodies from BPZE1-immunized mice to SCID mice. Clearance of Bordetella pertussis from the lungs was mediated by both BPZE1-induced antibodies and CD4(+), but not by CD8(+) T cells. The protective CD4(+) T cells comprised IFN-gamma-producing and IL-17-producing subsets, indicating that BPZE1 induces both Th1 and Th17 CD4(+) T cells. In addition, and in contrast to acellular pertussis vaccines, BPZE1 also cross-protected against Bordetella parapertussis infection, but in this case only the transfer of CD4(+) T cells conferred protection. Serum from BPZE1-immunized mice was not able to kill B. parapertussis and did not protect SCID mice against B. parapertussis infection. CONCLUSIONS/SIGNIFICANCE The novel live attenuated pertussis vaccine BPZE1 protects in a pre-clinical mouse model against B. pertussis challenge by both BPZE1-induced antibodies and CD4(+) T cell responses. It also protects against B. parapertussis infection. However, in this case protection is only T cell mediated.", "link"=>"http://www.mendeley.com/research/t-bcellmediated-protection-induced-novel-live-attenuated-pertussis-vaccine-mice-cross-protection-aga", "reader_count"=>27, "reader_count_by_academic_status"=>{"Researcher"=>10, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>3, "Other"=>4, "Student > Master"=>1, "Student > Bachelor"=>2, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Researcher"=>10, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>3, "Other"=>4, "Student > Master"=>1, "Student > Bachelor"=>2, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>16, "Medicine and Dentistry"=>6, "Immunology and Microbiology"=>3, "Economics, Econometrics and Finance"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>6}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>3}, "Economics, Econometrics and Finance"=>{"Economics, Econometrics and Finance"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>16}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}}, "reader_count_by_country"=>{"United States"=>2, "United Kingdom"=>1, "South Africa"=>1}, "group_count"=>2}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/853579"], "description"=>"<p>Two groups (BALB/c, white columns; and SCID, black columns) of 5 mice were infected i.n. with ∼1×10<sup>6 </sup><i>B. pertussis</i> BPSM and sacrificed 3 h (Day 0) or eight weeks later (Day 60) for CFU counts in the lungs. The results are expressed as means of log CFU values per lung (± standard error) and are representative of three independent experiments.</p>", "links"=>[], "tags"=>["causes", "persistent", "respiratory", "scid"], "article_id"=>524026, "categories"=>["Infectious Diseases", "Immunology", "Medicine"], "users"=>["Pascal Feunou Feunou", "Julie Bertout", "Camille Locht"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0010178.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_B_pertussis_causes_persistent_respiratory_infection_in_SCID_mice_/524026", "title"=>"<i>B. pertussis</i> causes persistent respiratory infection in SCID mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 01:45:58"}
  • {"files"=>["https://ndownloader.figshare.com/files/853630"], "description"=>"<p>100 µl of serum (<b>a</b>) or 50×10<sup>6</sup> whole spleen cells (WSC) (<b>b</b>) or indicated amounts of WSC (<b>c</b>) from non-immunized (Naïve) or BPZE1-immunized (BPZE1) BALB/c mice were transferred intraperitoneally to SCID mice 24 h before they were intranasally infected with virulent <i>B. pertussis</i> BPSM (1×10<sup>6</sup> CFU). Non-transferred SCID mice (No transfer) served as controls. The mice were sacrificed 7 days after challenge, and CFUs in the lungs were counted. The results are expressed as means of log CFU values per lung from 5 mice per group (± standard error) and are representative of three independent experiments.</p>", "links"=>[], "tags"=>["spleen", "cells", "serum", "bpze1-immunized"], "article_id"=>524081, "categories"=>["Infectious Diseases", "Immunology", "Medicine"], "users"=>["Pascal Feunou Feunou", "Julie Bertout", "Camille Locht"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0010178.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transfer_of_protection_by_spleen_cells_and_serum_from_BPZE1_immunized_mice_/524081", "title"=>"Transfer of protection by spleen cells and serum from BPZE1-immunized mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 01:46:23"}
  • {"files"=>["https://ndownloader.figshare.com/files/853728"], "description"=>"<p>BALB/c mice were left untreated (Naïve) or immunized intranasally with 10<sup>6</sup> CFU of <i>B. pertussis</i> BPZE1 (BPZE1). Eight weeks later, the mice were sacrificed and spleens of three individual mice per group were pooled and homogenized to obtain single-cell suspensions. 2.5×10<sup>6</sup> cells were cultured in triplicate overnight in the presence of 10 µg/ml PTX (PTX) or left unstimulated (Medium). Spleen-derived T cells were then labelled for surface expression of CD4 and CD8 (<b>a</b>), and intracellular IFN-γ expression by CD4<sup>+</sup> (<b>b</b>) and CD8<sup>+</sup> (<b>c</b>) T cells was assessed by flow cytometry. The results shown are representative of three independent experiments.</p>", "links"=>[], "tags"=>["phenotype", "cells", "bpze1"], "article_id"=>524186, "categories"=>["Infectious Diseases", "Immunology", "Medicine"], "users"=>["Pascal Feunou Feunou", "Julie Bertout", "Camille Locht"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0010178.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_CD4_T_phenotype_of_IFN_947_producing_T_cells_following_BPZE1_administration_/524186", "title"=>"CD4<sup>+</sup> T phenotype of IFN-γ-producing T cells following BPZE1 administration.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 01:46:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/853837"], "description"=>"<p>BALB/c mice were left untreated (Naïve) or immunized intranasally with 10<sup>6</sup> CFU of <i>B. pertussis</i> BPZE1 (BPZE1). Eight weeks later, the mice were sacrificed, and spleen cells were stimulated in triplicate in the presence (black bars) or absence (white bars) of 10 µg/ml FHA, with or without (Medium) 10 µg/ml anti-IL-10 or anti-IL-17 antibodies. After 60 h of culture, the secretion of IL-17 (<b>a</b>), IL-10 (<b>b</b>), IFN-γ (<b>d</b>) and IL-2 (<b>e</b>) in the culture supernatants was determined by sandwich ELISA, and the proliferation was assessed by [<sup>3</sup>H] thymidine incorporation (<b>c</b>). The results are the expressed as mean values (± standard error) for triplicate cultures from four mice per group and are representative of three experiments.</p>", "links"=>[], "tags"=>["splenocytes", "bpze1-immunized"], "article_id"=>524292, "categories"=>["Infectious Diseases", "Immunology", "Medicine"], "users"=>["Pascal Feunou Feunou", "Julie Bertout", "Camille Locht"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0010178.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cytokine_production_profile_of_splenocytes_from_BPZE1_immunized_mice_/524292", "title"=>"Cytokine production profile of splenocytes from BPZE1-immunized mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 01:47:30"}
  • {"files"=>["https://ndownloader.figshare.com/files/853927"], "description"=>"<p>BALB/c mice were left untreated (Naïve) or immunized intranasally with 10<sup>6</sup> CFU of <i>B. pertussis</i> BPZE1 (BPZE1). Eight weeks later, the mice were sacrificed, and spleens of three individual mice per group were pooled and homogenized to obtain single-cell suspensions. 2.5×10<sup>6</sup> cells were cultured in triplicate overnight in the presence (PTX) or absence (Medium) of 10 µg/ml PTX. Spleen-derived T cells were then labelled for surface expression of CD4, and intracellular IFN-γ and IL-17 expression was assessed by flow cytometry. The results are representative of three independent experiments.</p>", "links"=>[], "tags"=>["il-17", "produced", "subsets", "bpze1-immunized"], "article_id"=>524382, "categories"=>["Infectious Diseases", "Immunology", "Medicine"], "users"=>["Pascal Feunou Feunou", "Julie Bertout", "Camille Locht"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0010178.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_IFN_and_IL_17_are_produced_by_distinct_B_pertussis_specific_CD4_T_cell_subsets_from_BPZE1_immunized_mice_/524382", "title"=>"IFN-γ and IL-17 are produced by distinct <i>B. pertussis</i>-specific CD4<sup>+</sup> T cell subsets from BPZE1-immunized mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 01:48:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/854014"], "description"=>"<p>Purified CD4<sup>+</sup> or CD8<sup>+</sup> T cells from BPZE1-immunized BALB/c mice (<b>a</b>) were transferred intraperitoneally to naïve SCID mice 24 h before they were intranasally infected with virulent <i>B. pertussis</i> BPSM. The mice were sacrificed 8 days later, and CFUs present in the lungs were counted (<b>b</b>). The results are expressed as means of log CFU values per lung from 5 mice per group (± standard error) and are representative of two independent experiments.</p>", "links"=>[], "tags"=>["purified", "cells", "bpze1-immunized"], "article_id"=>524466, "categories"=>["Infectious Diseases", "Immunology", "Medicine"], "users"=>["Pascal Feunou Feunou", "Julie Bertout", "Camille Locht"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0010178.g006"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Protection_by_purified_CD4_T_cells_from_BPZE1_immunized_mice_/524466", "title"=>"Protection by purified CD4<sup>+</sup> T cells from BPZE1-immunized mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 01:48:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/854069"], "description"=>"<p>BALB/c mice were intranasally infected with 1×10<sup>6</sup> CFU of <i>B. pertussis</i> BPZE1 (BPZE1) or <i>B. parapertussis</i> (Bpp) or left untreated (Naïve). The mice were then challenged 8 weeks later with 1×10<sup>6</sup> CFU <i>B. parapertussis</i> and sacrificed 7 days after challenge for counting of CFUs in the lungs (<b>a</b>). 100 µl of sera (<b>b</b>) or 50×10<sup>6</sup> whole spleen cells (<b>c</b>) from BPZE1-immunized, <i>B. parapertussis</i>-infected or naïve mice were injected intraperitoneally into naive SCID mice. 24 h later, the mice were intranasally infected with <i>B. parapertussis</i> and sacrificed seven days later for CFU counts in the lungs. The results are expressed as means of log CFU values per lung from 5 mice per group (± standard error) and are representative of three independent experiments.</p>", "links"=>[], "tags"=>["immunity", "cross-protects"], "article_id"=>524525, "categories"=>["Infectious Diseases", "Immunology", "Medicine"], "users"=>["Pascal Feunou Feunou", "Julie Bertout", "Camille Locht"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0010178.g007"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_BPZE1_induced_immunity_cross_protects_against_Bordetella_parapertussis_/524525", "title"=>"BPZE1-induced immunity cross-protects against <i>Bordetella parapertussis</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 01:48:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/854156"], "description"=>"<p>300 CFU of <i>B. pertussis</i> (<b>a</b>) and <i>B. parapertussis</i> (<b>b</b>) were incubated at 37°C for 1 h in the presence of 100 µl of 80% serum from unimmunized (Naïve) or BPZE1-immunized (BPZE1) mice and then plated onto Bordet Gengou blood agar plates for CFU counting. The results are presented as percent survival relative to that of a PBS control (in the absence of serum) (± standard error) for 3 mice per group, evaluated on 3 different plates per mouse, and are representative of three independent experiments.</p>", "links"=>[], "tags"=>["serum", "non-immune", "bpze1-immunized"], "article_id"=>524607, "categories"=>["Infectious Diseases", "Immunology", "Medicine"], "users"=>["Pascal Feunou Feunou", "Julie Bertout", "Camille Locht"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0010178.g008"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Survival_of_B_pertussis_and_B_parapertussis_in_the_presence_of_serum_from_non_immune_or_BPZE1_immunized_mice_/524607", "title"=>"Survival of <i>B. pertussis</i> and <i>B. parapertussis</i> in the presence of serum from non-immune or BPZE1-immunized mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 01:49:26"}

PMC Usage Stats | Further Information

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