Phylogeny Disambiguates the Evolution of Heat-Shock cis-Regulatory Elements in Drosophila
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Phylogeny disambiguates the evolution of heat-shock cis-regulatory elements in Drosophila", "type"=>"journal", "authors"=>[{"first_name"=>"Sibo", "last_name"=>"Tian", "scopus_author_id"=>"36459076300"}, {"first_name"=>"Robert A.", "last_name"=>"Haney", "scopus_author_id"=>"36762582600"}, {"first_name"=>"Martin E.", "last_name"=>"Feder", "scopus_author_id"=>"7103362838"}], "year"=>2010, "source"=>"PLoS ONE", "identifiers"=>{"isbn"=>"1932-6203", "pmid"=>"20498853", "pui"=>"359515528", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0010669", "scopus"=>"2-s2.0-77956276065", "sgr"=>"77956276065"}, "id"=>"96271187-59e3-3e08-98b8-093234ea277f", "abstract"=>"Heat-shock genes have a well-studied control mechanism for their expression that is mediated through cis-regulatory motifs known as heat-shock elements (HSEs). The evolution of important features of this control mechanism has not been investigated in detail, however. Here we exploit the genome sequencing of multiple Drosophila species, combined with a wealth of available information on the structure and function of HSEs in D. melanogaster, to undertake this investigation. We find that in single-copy heat shock genes, entire HSEs have evolved or disappeared 14 times, and the phylogenetic approach bounds the timing and direction of these evolutionary events in relation to speciation. In contrast, in the multi-copy gene Hsp70, the number of HSEs is nearly constant across species. HSEs evolve in size, position, and sequence within heat-shock promoters. In turn, functional significance of certain features is implicated by preservation despite this evolutionary change; these features include tail-to-tail arrangements of HSEs, gapped HSEs, and the presence or absence of entire HSEs. The variation among Drosophila species indicates that the cis-regulatory encoding of responsiveness to heat and other stresses is diverse. The broad dimensions of variation uncovered are particularly important as they suggest a substantial challenge for functional studies.", "link"=>"http://www.mendeley.com/research/phylogeny-disambiguates-evolution-heatshock-cisregulatory-elements-drosophila", "reader_count"=>23, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>5, "Student > Doctoral Student"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>12, "Student > Master"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>5, "Student > Doctoral Student"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>12, "Student > Master"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Environmental Science"=>2, "Agricultural and Biological Sciences"=>21}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>21}, "Environmental Science"=>{"Environmental Science"=>2}}, "reader_count_by_country"=>{"United States"=>3, "Chile"=>1, "Germany"=>2}, "group_count"=>1}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/77956276065"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/77956276065?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77956276065&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=77956276065&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/77956276065", "dc:identifier"=>"SCOPUS_ID:77956276065", "eid"=>"2-s2.0-77956276065", "dc:title"=>"Phylogeny disambiguates the evolution of heat-shock cis-regulatory elements in Drosophila", "dc:creator"=>"Tian S.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"5", "prism:issueIdentifier"=>"5", "prism:pageRange"=>nil, "prism:coverDate"=>"2010-09-17", "prism:coverDisplayDate"=>"2010", "prism:doi"=>"10.1371/journal.pone.0010669", "citedby-count"=>"28", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Department of Organismal Biology and Anatomy, The University of Chicago", "affiliation-city"=>"Chicago", "affiliation-country"=>"United States"}], "pubmed-id"=>"20498853", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e10669", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0010669", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"5", "year"=>"2010", "pdf_views"=>"36", "xml_views"=>"5", "html_views"=>"108"}
  • {"month"=>"6", "year"=>"2010", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"7", "year"=>"2010", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"8", "year"=>"2010", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"9", "year"=>"2010", "pdf_views"=>"12", "xml_views"=>"2", "html_views"=>"30"}
  • {"month"=>"10", "year"=>"2010", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"11", "year"=>"2010", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"12", "year"=>"2010", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"1", "year"=>"2011", "pdf_views"=>"10", "xml_views"=>"2", "html_views"=>"24"}
  • {"month"=>"2", "year"=>"2011", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"3", "year"=>"2011", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"4", "year"=>"2011", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"5", "year"=>"2011", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"6", "year"=>"2011", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"7", "year"=>"2011", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2011", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"9", "year"=>"2011", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"10", "year"=>"2011", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"11", "year"=>"2011", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"5", "xml_views"=>"5", "html_views"=>"12"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"20"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"0", "xml_views"=>"2", "html_views"=>"17"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"0", "xml_views"=>"2", "html_views"=>"18"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"0", "xml_views"=>"2", "html_views"=>"6"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"3", "html_views"=>"12"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"17"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"10"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"74"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"85"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"89"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"93"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"18"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"3", "html_views"=>"23"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"3", "html_views"=>"5"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"28", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"10", "year"=>"2020", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"11", "year"=>"2020", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"6"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/848707"], "description"=>"<p>Numbers indicate length of HSE in 5 bp units. Rectangles represent HSEs, whose length (in 5 bp subunits) is indicated. HSEs of the same color indicate probable homologs. Each circle, arbitrarily indicates a deduced evolutionary gain (+) or loss (−) of an HSE, and is placed arbitrarily at the center of the segment in which it occurs. Below a heat map expresses the similarity of each 5 bp subunit to the canonical sequence: G represents nGAAn-type motifs, while T represents nTTCn-type motifs. Columns correspond to the order of HSEs (from left to right) in the promoter maps. Key to heat maps: the number represents the information content of each actual subunit as a percent of the value calculated for the consensus sequence. Genome alignment indicates all proximal HSEs of 11 species occur in a conserved block near the TSS, with the exception of <i>D. grimshawi</i>. These HSEs are colored yellow. Point substitutions and small indels give rise to length variation in HSEs and a small gap in <i>D. ananassae</i>. In <i>D. grimshawi</i>, two copies of <i>Hsp22</i> appear to be present. The copy whose coding sequence most closely aligns with those of the other 11 species lacks a conserved TSS or promoter and may not be expressed. Both a conserved TSS and promoter are present in the second copy, and included in the figure. A distal HSE is present in all species but <i>D. grimshawi</i>. These vary in size and distance from the TSS. However, all appear to be orthologous in whole genome alignments, as well as in local alignments with multiple algorithms, with the exception of those in <i>D. willistoni</i> and <i>D. mojavensis</i>. If correct, this implies parallel losses and re-evolutions of HSEs, which we reject by parsimony. The figure presents a simpler scenario in which only a single loss occurs. In <i>D. ananassae</i>, parsimony suggests that the two proximal HSEs arose from insertion into or mutation of a single ancestral HSE.</p>", "links"=>[], "tags"=>["hses", "13"], "article_id"=>519168, "categories"=>["Evolutionary Biology", "Virology", "Computational Biology"], "users"=>["Sibo Tian", "Robert A. Haney", "Martin E. Feder"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0010669.g002", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Organization_of_identified_HSEs_for_Hsp22_in_13_Drosophila_species_/519168", "title"=>"Organization of identified HSEs for <i>Hsp22</i> in 13 <i>Drosophila</i> species.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-05-17 02:32:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/849334"], "description"=>"<p>The number of HSEs is nearly constant across copies in all species. Proximal HSEs are nearly invariant in position, and differ minimally in length. There is a larger amount of variation in the position of distal HSEs, but only a single gain/loss of HSEs occurs, in one copy of <i>Hsp70</i> from <i>D. santomea</i>.</p>", "links"=>[], "tags"=>["positions", "hses", "copies", "13"], "article_id"=>519792, "categories"=>["Evolutionary Biology", "Virology", "Computational Biology"], "users"=>["Sibo Tian", "Robert A. Haney", "Martin E. Feder"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0010669.g008", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sizes_and_positions_of_identified_HSEs_for_all_identified_copies_of_Hsp70_in_13_Drosophila_species_/519792", "title"=>"Sizes and positions of identified HSEs for all identified copies of <i>Hsp70</i> in 13 <i>Drosophila</i> species.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-05-17 02:43:12"}
  • {"files"=>["https://ndownloader.figshare.com/files/848653"], "description"=>"<p>This cartoon, greatly simplified from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010669#pone.0010669-Littlefield1\" target=\"_blank\">[33]</a>, Fig. 11 of <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010669#pone.0010669-Kremer1\" target=\"_blank\">[13]</a>, and Fig. 4 of <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010669#pone.0010669-Zhao1\" target=\"_blank\">[31]</a>, depicts two HSEs, each comprising three consecutive 5 bp sequences of n<b>G</b>AAn and nTT<b>C</b>n in alternation and each binding an HSF trimer. The HSF monomers (mHSF) interact with one another via a trimerization domain and the “wing” of the winged helix-loop-helix motif. In HSF, Helix 3, whose sequence is conserved in eukaryotes, is responsible for the specificity of interaction with the HSEs. Once bound, HSF trimers interact with the transcriptional complex, including RNA polymerase II (Pol II) to result in gene expression. See <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010669#s1\" target=\"_blank\">Introduction</a> for additional explanation. Inset: symbolic representation of the promoter organization, as will appear in subsequent figures. Aspects omitted from the cartoon include HSEs longer than three 5 bp units, which support cooperative binding of HSF trimers, variation in sequence within HSEs, variation in position and spacing of HSEs, GAGA factor and its binding sites, other cis-regulatory elements, nucleosomes and their acetylation sites, and numerous proteins involved in transcriptional regulation.</p>", "links"=>[], "tags"=>["heat-shock", "trimers", "elements", "mediate", "transcription"], "article_id"=>519106, "categories"=>["Evolutionary Biology", "Virology", "Computational Biology"], "users"=>["Sibo Tian", "Robert A. Haney", "Martin E. Feder"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0010669.g001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Binding_of_Heat_Shock_Factor_HSF_trimers_to_Heat_Shock_Elements_HSEs_mediate_the_transcription_of_heat_shock_genes_/519106", "title"=>"Binding of Heat-Shock Factor (HSF) trimers to Heat Shock Elements (HSEs) mediate the transcription of heat-shock genes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-05-17 02:31:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/848806"], "description"=>"<p>Numbers indicate length of HSE in 5 bp units. Genome alignment indicates all proximal HSEs of 12 species occur in a conserved block near the TSS. These HSEs are colored yellow. Parsimony suggests that two proximal HSEs are ancestral, and their variation in relative size and spacing arose from point substitutions and small indels. The absence of one member of the cluster in only one of the 12 species (<i>D. willistoni)</i> is therefore considered a loss. A single gain of two distal HSEs in a common ancestor of the <i>melanogaster-obscura</i> groups, a gain of a third distal HSE n <i>D. ananassae</i> alone, and a gain of a single distal HSE in <i>D. mojavensis</i> are more parsimonious than alternative scenarios.</p>", "links"=>[], "tags"=>["positions", "hses", "13"], "article_id"=>519267, "categories"=>["Evolutionary Biology", "Virology", "Computational Biology"], "users"=>["Sibo Tian", "Robert A. Haney", "Martin E. Feder"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0010669.g003", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sizes_and_positions_of_identified_HSEs_for_Hsp23_in_13_Drosophila_species_/519267", "title"=>"Sizes and positions of identified HSEs for <i>Hsp23</i> in 13 <i>Drosophila</i> species.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-05-17 02:34:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/849857"], "description"=>"<p>Subunits were first separated, and tested independently. On the vertical axis, the four largest letters represent the first four positions in the consensus sequence, and the horizontal represent positions 2 through 5. Small letters indicate the pair of bases are tested. Results are summarized as the number of excesses of deficiencies found in each test. Key to heat map: numbers indicated deviation from expected number of observations.</p>", "links"=>[], "tags"=>["two-way", "chi-square"], "article_id"=>520320, "categories"=>["Evolutionary Biology", "Virology", "Computational Biology"], "users"=>["Sibo Tian", "Robert A. Haney", "Martin E. Feder"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0010669.g012", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Results_of_two_way_chi_square_tests_/520320", "title"=>"Results of two-way chi-square tests.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-05-17 00:05:20"}
  • {"files"=>["https://ndownloader.figshare.com/files/849009"], "description"=>"<p>In the <i>melanogaster</i> subgroup, it is the only heat-shock gene with no HSE within 200 bp of the TSS. The multispecies genome alignment provides support for a rearrangement in <i>D. ananassae</i> and one or more deletions in the <i>obscura</i> group that remove intervening sequence and explain differences in distance to the TSS. We conservatively assume a single origin of proximal HSE sequence in <i>Drosophila Hsp27</i> promoters, with subsequent modifications of length and distance to the TSS. A second, more distal HSE is found in all species, and appears to be orthologous in the <i>melanogaster</i> and <i>obscura</i> groups. A gain of a third distal HSE has occurred in the melanogaster subgroup. <i>D. willistoni</i> and <i>D. virilis</i> also have a third HSE, suggesting lineage specific gains. However, although the regions compared are directly adjacent to an orthologous coding region, ambiguity exists in the relationships of individual HSE in <i>D. willistoni</i> and the species of the subgenus <i>Drosophila</i>, and these HSEs are colored white.</p>", "links"=>[], "tags"=>["hses"], "article_id"=>519473, "categories"=>["Evolutionary Biology", "Virology", "Computational Biology"], "users"=>["Sibo Tian", "Robert A. Haney", "Martin E. Feder"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0010669.g005", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_distribution_of_HSEs_is_most_variable_in_H_s_p27_/519473", "title"=>"The distribution of HSEs is most variable in <i>H</i>s<i>p27</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-05-17 02:37:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/849443"], "description"=>"<p>Numbers indicate length of HSE in 5 bp units. Genome alignment indicates all proximal HSEs of 12 species occur in a conserved block near the TSS. These HSEs are colored yellow. Length variation in proximal HSEs is due to both point substitutions and small indels. A distal HSE in the subgenus <i>Drosophila</i> only is equally consistent with a single gain in this subgenus or a single loss of this HSE in Sophophora. Sequence of an outgroup (<i>Culex pipiens</i>) implicates the latter scenario by parsimony.</p>", "links"=>[], "tags"=>["positions", "hses", "13"], "article_id"=>519906, "categories"=>["Evolutionary Biology", "Virology", "Computational Biology"], "users"=>["Sibo Tian", "Robert A. Haney", "Martin E. Feder"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0010669.g009", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sizes_and_positions_of_identified_HSEs_for_Hsp83_in_13_Drosophila_species_/519906", "title"=>"Sizes and positions of identified HSEs for <i>Hsp83</i> in 13 <i>Drosophila</i> species.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-05-17 02:45:06"}
  • {"files"=>["https://ndownloader.figshare.com/files/422531", "https://ndownloader.figshare.com/files/422548"], "description"=>"<div><p>Heat-shock genes have a well-studied control mechanism for their expression that is mediated through <em>cis</em>-regulatory motifs known as heat-shock elements (HSEs). The evolution of important features of this control mechanism has not been investigated in detail, however. Here we exploit the genome sequencing of multiple <em>Drosophila</em> species, combined with a wealth of available information on the structure and function of HSEs in <em>D. melanogaster</em>, to undertake this investigation. We find that in single-copy heat shock genes, entire HSEs have evolved or disappeared 14 times, and the phylogenetic approach bounds the timing and direction of these evolutionary events in relation to speciation. In contrast, in the multi-copy gene <em>Hsp70</em>, the number of HSEs is nearly constant across species. HSEs evolve in size, position, and sequence within heat-shock promoters. In turn, functional significance of certain features is implicated by preservation despite this evolutionary change; these features include tail-to-tail arrangements of HSEs, gapped HSEs, and the presence or absence of entire HSEs. The variation among <em>Drosophila</em> species indicates that the <em>cis</em>-regulatory encoding of responsiveness to heat and other stresses is diverse. The broad dimensions of variation uncovered are particularly important as they suggest a substantial challenge for functional studies.</p></div>", "links"=>[], "tags"=>["phylogeny", "disambiguates", "heat-shock", "elements"], "article_id"=>143439, "categories"=>["Evolutionary Biology", "Cancer", "Biological Sciences"], "users"=>["Sibo Tian", "Robert A. Haney", "Martin E. Feder"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0010669.s001", "https://dx.doi.org/10.1371/journal.pone.0010669.s002"], "stats"=>{"downloads"=>8, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Phylogeny_Disambiguates_the_Evolution_of_Heat_Shock_cis_Regulatory_Elements_in_Drosophila_/143439", "title"=>"Phylogeny Disambiguates the Evolution of Heat-Shock <em>cis</em>-Regulatory Elements in <em>Drosophila</em>", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2010-05-17 00:57:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/849562"], "description"=>"<p>Tallies of total changes in all 7 genes surveyed are placed in the centers of the segments in which the changes occurred. The changes themselves, however, may have occurred at any time during the interval the segment represents. *One change, that for <i>Hsp68</i>, cannot be assigned to a specific segment because the published genomic sequence for <i>D. willistoni</i> lacks a proximal promoter region that can be aligned to those for the other species.</p>", "links"=>[], "tags"=>["changes", "numbers", "hses", "superimposed", "phylogenetic", "13"], "article_id"=>520016, "categories"=>["Evolutionary Biology", "Virology", "Computational Biology"], "users"=>["Sibo Tian", "Robert A. Haney", "Martin E. Feder"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0010669.g010", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Evolutionary_changes_in_numbers_of_HSEs_superimposed_on_a_phylogenetic_tree_of_the_13_species_/520016", "title"=>"Evolutionary changes in numbers of HSEs superimposed on a phylogenetic tree of the 13 species.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-05-17 00:00:16"}
  • {"files"=>["https://ndownloader.figshare.com/files/849228"], "description"=>"<p>Numbers indicate length of HSE in 5 bp units. Multispecies genome alignment juxtaposes <i>Hsp68</i> and <i>Hsp70</i> copies in some species. Hence, all coding regions called as <i>Hsp68</i> in this study were confirmed through amino-acid states at diagnostic sites following Kellet and McKechnie (2005), and local alignments were used to assess conservation in some species. No recognizable copy of <i>Hsp68</i> was found in <i>D. willistoni</i>. All proximal HSEs occur in a conserved block near the TSS. Upstream regions are divergent, and no distal HSEs are recognized outside the <i>melanogaster</i> and <i>obscura</i> groups. High levels of sequence divergence in the regions including and flanking the distal HSEs ambiguate the orthology of the HSEs between the <i>melanogaster</i> and <i>obscura</i> groups. Parsimony implicates a single gain of a distal HSE rather than two independent gains. Finally, the distal HSE could have arisen in the common ancestor of the <i>melanogaster</i> and <i>obscura</i> groups, or it could have arisen in the common ancestor of all species and been lost in the common ancestor of <i>D. mojavensis</i>, <i>D. virilis</i>, and <i>D. grimshawi</i>. Parsimony supports the former scenario.</p>", "links"=>[], "tags"=>["positions", "hses", "13"], "article_id"=>519694, "categories"=>["Evolutionary Biology", "Virology", "Computational Biology"], "users"=>["Sibo Tian", "Robert A. Haney", "Martin E. Feder"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0010669.g007", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sizes_and_positions_of_identified_HSEs_for_Hsp68_in_13_Drosophila_species_/519694", "title"=>"Sizes and positions of identified HSEs for <i>Hsp68</i> in 13 <i>Drosophila</i> species.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-05-17 02:41:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/848900"], "description"=>"<p>Numbers indicate length of HSE in 5 bp units. Genome alignment indicates all proximal HSEs of 12 species occur in a conserved block near the TSS. These HSEs are colored yellow. Length variation in proximal HSEs is due to both point substitutions and small indels. By parsimony, an upstream distal HSE cluster arose in a common ancestor of the <i>melanogaster</i> subgroup, and a single distal HSE arose independently in <i>D. willistoni</i> and <i>D. virilis</i>. The distal element in <i>D. willistoni</i> appears due to a local duplication of sequence proximal to the TSS. Duplication of the <i>Hsp26</i> coding sequence appears to have occurred in <i>D. mojavensis</i> with both copies having a similar proximal promoter architecture.</p>", "links"=>[], "tags"=>["positions", "hses", "13"], "article_id"=>519356, "categories"=>["Evolutionary Biology", "Virology", "Computational Biology"], "users"=>["Sibo Tian", "Robert A. Haney", "Martin E. Feder"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0010669.g004", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sizes_and_positions_of_identified_HSEs_for_Hsp26_in_13_Drosophila_species_/519356", "title"=>"Sizes and positions of identified HSEs for <i>Hsp26</i> in 13 <i>Drosophila</i> species.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-05-17 02:35:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/849661"], "description"=>"<p>WebLogos were generated using the entire pool of available motifs, including duplications and multiple <i>Hsp70</i> copies.</p>", "links"=>[], "tags"=>["logos", "ngaan", "nttcn"], "article_id"=>520121, "categories"=>["Evolutionary Biology", "Virology", "Computational Biology"], "users"=>["Sibo Tian", "Robert A. Haney", "Martin E. Feder"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0010669.g011", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sequence_logos_for_nGAAn_and_nTTCn_motifs_/520121", "title"=>"Sequence logos for nGAAn and nTTCn motifs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-05-17 00:02:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/849107"], "description"=>"<p>Numbers indicate length of HSE in 5 bp units. Genome alignment indicates all proximal HSEs of 12 species occur in a conserved block near the TSS, with the exception of <i>D. willistoni</i>. However, local alignments upstream of the DNAJ-1 ortholog in this species indicate conservation of this HSE. Conserved proximal HSEs are colored yellow. Minor length variation in proximal HSEs are presumably due to small indels and point substitutions. Although divergence in the <i>D. ananassae</i> promoter region ambiguates the orthology of its distal HSE, its location, size, and parsimony suggest a single origin of a distal HSEs in a common ancestor of the <i>melanogaster</i> group. Distal HSEs arose independently in the <i>obscura</i> group and in <i>D. grimshawi</i>.</p>", "links"=>[], "tags"=>["positions", "hses", "13"], "article_id"=>519567, "categories"=>["Evolutionary Biology", "Virology", "Computational Biology"], "users"=>["Sibo Tian", "Robert A. Haney", "Martin E. Feder"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0010669.g006", "stats"=>{"downloads"=>2, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sizes_and_positions_of_identified_HSEs_for_DNAJ_1_in_13_Drosophila_species_/519567", "title"=>"Sizes and positions of identified HSEs for <i>DNAJ-1</i> in 13 <i>Drosophila</i> species.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-05-17 02:39:27"}

PMC Usage Stats | Further Information

  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"8", "unique-ip"=>"8", "pdf"=>"5", "year"=>"2010", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"35", "year"=>"2010", "pdf"=>"25", "unique-ip"=>"37", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"4"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"8", "unique-ip"=>"11", "pdf"=>"8", "year"=>"2010", "figure"=>"6", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"8", "year"=>"2010", "pdf"=>"5", "unique-ip"=>"9", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"18", "unique-ip"=>"20", "pdf"=>"17", "year"=>"2010", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"16", "year"=>"2010", "pdf"=>"9", "unique-ip"=>"15", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"13", "unique-ip"=>"10", "pdf"=>"4", "year"=>"2010", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"6", "year"=>"2010", "pdf"=>"2", "unique-ip"=>"7", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"12", "unique-ip"=>"13", "pdf"=>"6", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"16", "year"=>"2011", "pdf"=>"2", "unique-ip"=>"17", "figure"=>"6", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"5", "full-text"=>"10", "unique-ip"=>"18", "pdf"=>"3", "year"=>"2011", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"12", "year"=>"2011", "pdf"=>"7", "unique-ip"=>"14", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"1", "abstract"=>"1", "full-text"=>"6", "unique-ip"=>"10", "pdf"=>"7", "year"=>"2011", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"3"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"5", "year"=>"2011", "pdf"=>"8", "unique-ip"=>"14", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"1", "unique-ip"=>"2", "pdf"=>"0", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"1", "abstract"=>"0", "full-text"=>"28", "year"=>"2011", "pdf"=>"9", "unique-ip"=>"11", "figure"=>"10", "scanned-summary"=>"0", "supp-data"=>"3"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"4", "unique-ip"=>"3", "pdf"=>"1", "year"=>"2011", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"4"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"5", "year"=>"2011", "pdf"=>"1", "unique-ip"=>"5", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"3", "unique-ip"=>"4", "pdf"=>"1", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"4", "year"=>"2011", "pdf"=>"2", "unique-ip"=>"3", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"3", "unique-ip"=>"2", "pdf"=>"1", "year"=>"2012", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"2", "year"=>"2012", "pdf"=>"2", "unique-ip"=>"3", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"6", "unique-ip"=>"9", "pdf"=>"3", "year"=>"2012", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"3", "year"=>"2012", "pdf"=>"2", "unique-ip"=>"6", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"3", "unique-ip"=>"8", "pdf"=>"4", "year"=>"2012", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"2", "year"=>"2012", "pdf"=>"0", "unique-ip"=>"4", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"3", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"15", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"11", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"1", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"19", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"42", "full-text"=>"21", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"5", "cited-by"=>"1", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"2", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"0", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"10"}

Relative Metric

{"start_date"=>"2010-01-01T00:00:00Z", "end_date"=>"2010-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Computational biology", "average_usage"=>[334, 662, 847, 1004, 1136, 1245, 1346, 1439, 1513, 1595, 1657, 1726, 1787, 1853, 1905, 1972, 2049, 2112, 2182, 2254, 2335, 2411, 2473, 2537, 2587, 2662, 2729, 2786, 2849, 2932, 2997, 3055, 3122, 3190, 3270, 3331, 3398, 3472, 3534, 3611, 3684, 3766, 3814, 3865, 3909, 3956, 4011, 4073, 4112]}, {"subject_area"=>"/Biology and life sciences/Evolutionary biology", "average_usage"=>[342, 639, 812, 953, 1062, 1178, 1282, 1358, 1452, 1515, 1604, 1668, 1734, 1819, 1896, 1947, 2009, 2062, 2128, 2213, 2269, 2350, 2422, 2490, 2565, 2629, 2699, 2768, 2834, 2909, 2962, 3034, 3097, 3167, 3232, 3321, 3372, 3439, 3496, 3534, 3598, 3659, 3715, 3780, 3845, 3903, 3992, 4051, 4123]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[306, 610, 768, 915, 1043, 1164, 1255, 1349, 1432, 1511, 1592, 1674, 1744, 1817, 1889, 1950, 2028, 2087, 2153, 2217, 2274, 2349, 2418, 2488, 2558, 2616, 2686, 2745, 2809, 2869, 2938, 3008, 3071, 3131, 3189, 3258, 3320, 3389, 3446, 3503, 3552, 3613, 3677, 3744, 3788, 3842, 3907, 3966, 4015]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[286, 573, 735, 872, 989, 1096, 1185, 1277, 1365, 1449, 1520, 1597, 1668, 1729, 1799, 1852, 1917, 1984, 2054, 2108, 2169, 2234, 2303, 2374, 2436, 2501, 2560, 2620, 2683, 2742, 2830, 2909, 2981, 3032, 3089, 3148, 3210, 3266, 3323, 3379, 3448, 3512, 3576, 3635, 3693, 3747, 3827, 3876, 3938]}, {"subject_area"=>"/Computer and information sciences", "average_usage"=>[375, 666, 837, 949, 1062, 1171, 1233, 1318, 1399, 1470, 1550, 1608, 1701, 1771, 1835, 1908, 1973, 2046, 2091, 2160, 2220, 2285, 2359, 2455, 2537, 2610, 2687, 2778, 2836, 2908, 2974, 3021, 3082, 3169, 3224, 3300, 3382, 3469, 3550, 3640, 3728, 3765, 3814, 3858, 3915, 3952, 3991, 4067, 4107]}]}
Loading … Spinner
There are currently no alerts