Parthenogenic Blastocysts Derived from Cumulus-Free In Vitro Matured Human Oocytes
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{"title"=>"Parthenogenic blastocysts derived from cumulus-free In Vitro matured human oocytes", "type"=>"journal", "authors"=>[{"first_name"=>"Sohyun L.", "last_name"=>"McElroy", "scopus_author_id"=>"35969200900"}, {"first_name"=>"James A.", "last_name"=>"Byrne", "scopus_author_id"=>"7402801871"}, {"first_name"=>"Shawn L.", "last_name"=>"Chavez", "scopus_author_id"=>"24461301500"}, {"first_name"=>"Barry", "last_name"=>"Behr", "scopus_author_id"=>"7003959268"}, {"first_name"=>"Aaron J.", "last_name"=>"Hsueh", "scopus_author_id"=>"56262915100"}, {"first_name"=>"Lynn M.", "last_name"=>"Westphal", "scopus_author_id"=>"7003748779"}, {"first_name"=>"Renee A.", "last_name"=>"Reijo Pera", "scopus_author_id"=>"56777776600"}], "year"=>2010, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "sgr"=>"77956193967", "doi"=>"10.1371/journal.pone.0010979", "scopus"=>"2-s2.0-77956193967", "pui"=>"359289663", "pmid"=>"20539753"}, "id"=>"28656540-3b2f-31fa-b088-ce90209a97f2", "abstract"=>"BACKGROUND: Approximately 20% of oocytes are classified as immature and discarded following intracytoplasmic sperm injection (ICSI) procedures. These oocytes are obtained from gonadotropin-stimulated patients, and are routinely removed from the cumulus cells which normally would mature the oocytes. Given the ready access to these human oocytes, they represent a potential resource for both clinical and basic science application. However culture conditions for the maturation of cumulus-free oocytes have not been optimized. We aimed to improve maturation conditions for cumulus-free oocytes via culture with ovarian paracrine/autocrine factors identified by single cell analysis.\\n\\nMETHODOLOGY/PRINCIPAL FINDING: Immature human oocytes were matured in vitro via supplementation with ovarian paracrine/autocrine factors that were selected based on expression of ligands in the cumulus cells and their corresponding receptors in oocytes. Matured oocytes were artificially activated to assess developmental competence. Gene expression profiles of parthenotes were compared to IVF/ICSI embryos at morula and blastocyst stages. Following incubation in medium supplemented with ovarian factors (BDNF, IGF-I, estradiol, GDNF, FGF2 and leptin), a greater percentage of oocytes demonstrated nuclear maturation and subsequently, underwent parthenogenesis relative to control. Similarly, cytoplasmic maturation was also improved as indicated by development to blastocyst stage. Parthenogenic blastocysts exhibited mRNA expression profiles similar to those of blastocysts obtained after IVF/ICSI with the exception for MKLP2 and PEG1.\\n\\nCONCLUSIONS/SIGNIFICANCE: Human cumulus-free oocytes from hormone-stimulated cycles are capable of developing to blastocysts when cultured with ovarian factor supplementation. Our improved IVM culture conditions may be used for obtaining mature oocytes for clinical purposes and/or for derivation of embryonic stem cells following parthenogenesis or nuclear transfer.", "link"=>"http://www.mendeley.com/research/parthenogenic-blastocysts-derived-cumulusfree-vitro-matured-human-oocytes", "reader_count"=>27, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Student > Doctoral Student"=>3, "Researcher"=>7, "Student > Ph. D. Student"=>4, "Other"=>4, "Student > Master"=>4, "Professor"=>2, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Student > Doctoral Student"=>3, "Researcher"=>7, "Student > Ph. D. Student"=>4, "Other"=>4, "Student > Master"=>4, "Professor"=>2, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>5, "Agricultural and Biological Sciences"=>16, "Medicine and Dentistry"=>5, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>5}, "Chemistry"=>{"Chemistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>16}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>5}}, "reader_count_by_country"=>{"Canada"=>1, "Iran"=>1, "United States"=>2}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/846799"], "description"=>"<p>Sage: commercial IVM medium supplemented with 10% SPS, FSH, hCG and estradiol.</p><p>IVM-medium: IVM-medium supplemented with 10% SPS.</p><p>Supplement: IVM-medium supplemented with 10% SPS, BDNF, estradiol, IGF-I, GDNF, FGF2, leptin.</p><p>GV: germinal vesicle stage.</p><p>MI: metaphase I stage.</p><p>Nuclear maturation: the presence of a first polar body.</p>a,b<p>Within columns, values with different superscripts were significantly different (P<0.05).</p>", "links"=>[], "tags"=>["maturation", "cumulus-free", "oocytes", "hormone-stimulated"], "article_id"=>517249, "categories"=>["Cell Biology", "Mathematics", "Medicine", "Developmental Biology"], "users"=>["Sohyun L. McElroy", "James A. Byrne", "Shawn L. Chavez", "Barry Behr", "Aaron J. Hsueh", "Lynn M. Westphal", "Renee A. Reijo Pera"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0010979.t001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Nuclear_maturation_of_cumulus_free_human_oocytes_in_vitro_from_hormone_stimulated_cycle_/517249", "title"=>"Nuclear maturation of cumulus-free human oocytes <i>in vitro</i> from hormone-stimulated cycle.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2010-06-07 02:00:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/846695"], "description"=>"<p>The average mRNA expression level of morulas (A) and blastocysts (B) from parthenogenesis and IVF/ICSI were displayed. Red color: Significantly differentially expressed. PM = parthenogenic morula, PBL = parthenogenic blastocyst.</p>", "links"=>[], "tags"=>["parthenogenic"], "article_id"=>517150, "categories"=>["Cell Biology", "Mathematics", "Medicine", "Developmental Biology"], "users"=>["Sohyun L. McElroy", "James A. Byrne", "Shawn L. Chavez", "Barry Behr", "Aaron J. Hsueh", "Lynn M. Westphal", "Renee A. Reijo Pera"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0010979.g004", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Gene_expression_of_parthenogenic_embryos_/517150", "title"=>"Gene expression of parthenogenic embryos.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-06-07 01:59:10"}
  • {"files"=>["https://ndownloader.figshare.com/files/846446"], "description"=>"<p>(A) Ovarian paracrine/autocrine ligands, (B) their cognate receptors, and (C) schematic representation of gene expressions. Values are the means of different stages of oocytes including GV (N = 10), MI (N = 8), MII (N = 9), IVM-MII (N = 10) and cumulus cells (N = 5). Error bars represent standard deviation (SD). CNRQ = calculated normalized relative quantity. *: No detected in all samples. <sup>a,b</sup> or <sup>c,d</sup>: Values were significantly different (P<0.05), underline: low expression level, #: adopted from Hillier et al. <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010979#pone.0010979-Hillier1\" target=\"_blank\">[46]</a>.</p>", "links"=>[], "tags"=>["ovarian", "factors", "cognate", "receptors", "supplementation", "maturation"], "article_id"=>516897, "categories"=>["Cell Biology", "Mathematics", "Medicine", "Developmental Biology"], "users"=>["Sohyun L. McElroy", "James A. Byrne", "Shawn L. Chavez", "Barry Behr", "Aaron J. Hsueh", "Lynn M. Westphal", "Renee A. Reijo Pera"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0010979.g002", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expressions_of_ovarian_paracrine_autocrine_factors_and_their_cognate_receptors_selected_for_supplementation_in_in_vitro_maturation_media_/516897", "title"=>"Expressions of ovarian paracrine/autocrine factors and their cognate receptors selected for supplementation in <i>in vitro</i> maturation media.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-06-07 01:54:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/846592"], "description"=>"<p>PM = parthenogenic morula, PBL = parthenogenic blastocyst.</p>", "links"=>[], "tags"=>["morulas", "blastocysts", "parthenogenesis"], "article_id"=>517048, "categories"=>["Cell Biology", "Mathematics", "Medicine", "Developmental Biology"], "users"=>["Sohyun L. McElroy", "James A. Byrne", "Shawn L. Chavez", "Barry Behr", "Aaron J. Hsueh", "Lynn M. Westphal", "Renee A. Reijo Pera"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0010979.g003", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Treeview_display_of_cluster_analysis_of_gene_expression_of_all_morulas_and_blastocysts_from_parthenogenesis_and_IVF_ICSI_/517048", "title"=>"Treeview display of cluster analysis of gene expression of all morulas and blastocysts from parthenogenesis and IVF/ICSI.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-06-07 01:57:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/846838"], "description"=>"<p>Sage: commercial IVM medium supplemented with FSH, hCG and estradiol.</p><p>IVM-medium: IVM-medium supplemented with 10% SPS.</p><p>Supplement: IVM-medium supplemented with BDNF, estradiol, IGF-I, GDNF, FGF2, leptin.</p><p>*Percentage from activated oocytes.</p><p>**Percentage from cleaved embryos.</p>a,b<p>Within columns, values with different superscripts were significantly different (P<0.05).</p>", "links"=>[], "tags"=>["parthenogenetic", "activation", "oocytes", "matured"], "article_id"=>517290, "categories"=>["Cell Biology", "Mathematics", "Medicine", "Developmental Biology"], "users"=>["Sohyun L. McElroy", "James A. Byrne", "Shawn L. Chavez", "Barry Behr", "Aaron J. Hsueh", "Lynn M. Westphal", "Renee A. Reijo Pera"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0010979.t002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Embryo_development_after_parthenogenetic_activation_of_human_oocytes_matured_in_vitro_/517290", "title"=>"Embryo development after parthenogenetic activation of human oocytes matured <i>in vitro</i>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2010-06-07 02:01:30"}
  • {"files"=>["https://ndownloader.figshare.com/files/846375"], "description"=>"<p>Values are the means of different stages of oocytes including GV (N = 10), MI (N = 8), MII (N = 9), IVM-MII (N = 10) and cumulus cells (N = 5). Error bars represent standard deviation (SD). CNRQ: calculated normalized relative quantity. *: Not detected in all samples. <sup>a,b</sup> or <sup>c,d</sup>: Values were significantly different (P<0.05).</p>", "links"=>[], "tags"=>["oocyte-specific", "cumulus", "cell-specific"], "article_id"=>516829, "categories"=>["Cell Biology", "Mathematics", "Medicine", "Developmental Biology"], "users"=>["Sohyun L. McElroy", "James A. Byrne", "Shawn L. Chavez", "Barry Behr", "Aaron J. Hsueh", "Lynn M. Westphal", "Renee A. Reijo Pera"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0010979.g001", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expressions_of_oocyte_specific_or_cumulus_cell_specific_genes_/516829", "title"=>"Expressions of oocyte-specific or cumulus cell-specific genes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-06-07 01:53:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/421287", "https://ndownloader.figshare.com/files/421299", "https://ndownloader.figshare.com/files/421319", "https://ndownloader.figshare.com/files/421332"], "description"=>"<div><h3>Background</h3><p>Approximately 20% of oocytes are classified as immature and discarded following intracytoplasmic sperm injection (ICSI) procedures. These oocytes are obtained from gonadotropin-stimulated patients, and are routinely removed from the cumulus cells which normally would mature the oocytes. Given the ready access to these human oocytes, they represent a potential resource for both clinical and basic science application. However culture conditions for the maturation of cumulus-free oocytes have not been optimized. We aimed to improve maturation conditions for cumulus-free oocytes via culture with ovarian paracrine/autocrine factors identified by single cell analysis.</p><h3>Methodology/Principal Finding</h3><p>Immature human oocytes were matured <em>in vitro</em> via supplementation with ovarian paracrine/autocrine factors that were selected based on expression of ligands in the cumulus cells and their corresponding receptors in oocytes. Matured oocytes were artificially activated to assess developmental competence. Gene expression profiles of parthenotes were compared to IVF/ICSI embryos at morula and blastocyst stages. Following incubation in medium supplemented with ovarian factors (BDNF, IGF-I, estradiol, GDNF, FGF2 and leptin), a greater percentage of oocytes demonstrated nuclear maturation and subsequently, underwent parthenogenesis relative to control. Similarly, cytoplasmic maturation was also improved as indicated by development to blastocyst stage. Parthenogenic blastocysts exhibited mRNA expression profiles similar to those of blastocysts obtained after IVF/ICSI with the exception for <em>MKLP2</em> and <em>PEG1</em>.</p><h3>Conclusions/Significance</h3><p>Human cumulus-free oocytes from hormone-stimulated cycles are capable of developing to blastocysts when cultured with ovarian factor supplementation. Our improved IVM culture conditions may be used for obtaining mature oocytes for clinical purposes and/or for derivation of embryonic stem cells following parthenogenesis or nuclear transfer.</p></div>", "links"=>[], "tags"=>["parthenogenic", "blastocysts", "derived", "cumulus-free", "matured", "oocytes"], "article_id"=>143185, "categories"=>["Cell Biology", "Mathematics", "Medicine", "Developmental Biology"], "users"=>["Sohyun L. McElroy", "James A. Byrne", "Shawn L. Chavez", "Barry Behr", "Aaron J. Hsueh", "Lynn M. Westphal", "Renee A. Reijo Pera"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0010979.s001", "https://dx.doi.org/10.1371/journal.pone.0010979.s002", "https://dx.doi.org/10.1371/journal.pone.0010979.s003", "https://dx.doi.org/10.1371/journal.pone.0010979.s004"], "stats"=>{"downloads"=>3, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Parthenogenic_Blastocysts_Derived_from_Cumulus_Free_In_Vitro_Matured_Human_Oocytes/143185", "title"=>"Parthenogenic Blastocysts Derived from Cumulus-Free <em>In Vitro</em> Matured Human Oocytes", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2010-06-07 00:53:05"}

PMC Usage Stats | Further Information

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