Iterative Structure-Based Peptide-Like Inhibitor Design against the Botulinum Neurotoxin Serotype A
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Iterative structure-based peptide-like inhibitor design against the botulinum neurotoxin serotype A", "type"=>"journal", "authors"=>[{"first_name"=>"Jorge E.", "last_name"=>"Zuniga", "scopus_author_id"=>"9840651700"}, {"first_name"=>"Jared T.", "last_name"=>"Hammill", "scopus_author_id"=>"36846956800"}, {"first_name"=>"Omri", "last_name"=>"Drory", "scopus_author_id"=>"8701987300"}, {"first_name"=>"Jonathan E.", "last_name"=>"Nuss", "scopus_author_id"=>"35737826100"}, {"first_name"=>"James C.", "last_name"=>"Burnett", "scopus_author_id"=>"7202810656"}, {"first_name"=>"Rick", "last_name"=>"Gussio", "scopus_author_id"=>"6603670668"}, {"first_name"=>"Peter", "last_name"=>"Wipf", "scopus_author_id"=>"7102949331"}, {"first_name"=>"Sina", "last_name"=>"Bavari", "scopus_author_id"=>"7003364755"}, {"first_name"=>"Axel T.", "last_name"=>"Brunger", "scopus_author_id"=>"7103319400"}], "year"=>2010, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-77956224661", "sgr"=>"77956224661", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0011378", "pmid"=>"20614028", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pui"=>"359306526"}, "id"=>"c691a85b-2d1b-3aee-b3da-b908d7ec58c7", "abstract"=>"The botulinum neurotoxin serotype A light chain (BoNT/A LC) protease is the catalytic component responsible for the neuroparalysis that is characteristic of the disease state botulism. Three related peptide-like molecules (PLMs) were designed using previous information from co-crystal structures, synthesized, and assayed for in vitro inhibition against BoNT/A LC. Our results indicate these PLMS are competitive inhibitors of the BoNT/A LC protease and their K(i) values are in the nM-range. A co-crystal structure for one of these inhibitors was determined and reveals that the PLM, in accord with the goals of our design strategy, simultaneously involves both ionic interactions via its P1 residue and hydrophobic contacts by means of an aromatic group in the P2' position. The PLM adopts a helical conformation similar to previously determined co-crystal structures of PLMs, although there are also major differences to these other structures such as contacts with specific BoNT/A LC residues. Our structure further demonstrates the remarkable plasticity of the substrate binding cleft of the BoNT/A LC protease and provides a paradigm for iterative structure-based design and development of BoNT/A LC inhibitors.", "link"=>"http://www.mendeley.com/research/iterative-structurebased-peptidelike-inhibitor-design-against-botulinum-neurotoxin-serotype", "reader_count"=>21, "reader_count_by_academic_status"=>{"Researcher"=>8, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>2, "Student > Master"=>2, "Student > Bachelor"=>1, "Professor > Associate Professor"=>1}, "reader_count_by_user_role"=>{"Researcher"=>8, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>2, "Student > Master"=>2, "Student > Bachelor"=>1, "Professor > Associate Professor"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>1, "Materials Science"=>1, "Agricultural and Biological Sciences"=>10, "Medicine and Dentistry"=>1, "Arts and Humanities"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Chemistry"=>4, "Computer Science"=>1, "Neuroscience"=>1}, "reader_count_by_subdiscipline"=>{"Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>4}, "Neuroscience"=>{"Neuroscience"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>10}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "Arts and Humanities"=>{"Arts and Humanities"=>1}}, "reader_count_by_country"=>{"France"=>2, "Germany"=>1}, "group_count"=>2}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/77956224661"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/77956224661?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77956224661&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=77956224661&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/77956224661", "dc:identifier"=>"SCOPUS_ID:77956224661", "eid"=>"2-s2.0-77956224661", "dc:title"=>"Iterative structure-based peptide-like inhibitor design against the botulinum neurotoxin serotype A", "dc:creator"=>"Zuniga J.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"5", "prism:issueIdentifier"=>"6", "prism:pageRange"=>nil, "prism:coverDate"=>"2010-08-12", "prism:coverDisplayDate"=>"2010", "prism:doi"=>"10.1371/journal.pone.0011378", "citedby-count"=>"20", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Stanford University", "affiliation-city"=>"Palo Alto", "affiliation-country"=>"United States"}], "pubmed-id"=>"20614028", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e11378", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0011378", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"6", "year"=>"2010", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"7", "year"=>"2010", "pdf_views"=>"112", "xml_views"=>"13", "html_views"=>"358"}
  • {"month"=>"8", "year"=>"2010", "pdf_views"=>"24", "xml_views"=>"1", "html_views"=>"72"}
  • {"month"=>"9", "year"=>"2010", "pdf_views"=>"29", "xml_views"=>"3", "html_views"=>"105"}
  • {"month"=>"10", "year"=>"2010", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"73"}
  • {"month"=>"11", "year"=>"2010", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"12", "year"=>"2010", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"50"}
  • {"month"=>"1", "year"=>"2011", "pdf_views"=>"9", "xml_views"=>"2", "html_views"=>"56"}
  • {"month"=>"2", "year"=>"2011", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"3", "year"=>"2011", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"4", "year"=>"2011", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"5", "year"=>"2011", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"6", "year"=>"2011", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"7", "year"=>"2011", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"8", "year"=>"2011", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"9", "year"=>"2011", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"40"}
  • {"month"=>"10", "year"=>"2011", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"11", "year"=>"2011", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"10", "html_views"=>"21"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"45"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"41"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"16"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"23"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"288"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"83"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"70"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"112"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"96"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"74"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"25"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"3", "html_views"=>"3"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"1"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/842335"], "description"=>"<p>Superposition of the P2′ Phe of the <b>JTH-NB72-39</b> (tan sticks) and the P2′ Trp of <b>I1</b> (grey sticks) in complex with BoNT/A LC. The van der Waals surfaces (dots) of the side-chain atoms of the P2′ Phe and P2′ Trp illustrate the steric effect of these aromatic moieties. BoNT/A LC residues are colored cyan and blue for the <b>JTH-NB72-39</b>-bound and the <b>I1</b>-bound models, respectively. Phe369 and Leu256 of the BoNT/A LC are labeled with their one-letter code and number in the BoNT/A LC sequence, and their side chains are displayed in stick representation. <b>B</b>. A side view of the interactions shown in Panel A. The backbone atoms of the P3′Aib residue of <b>JTH-NB72-39</b> are colored orange. The Zn(II) ion is displayed as a yellow sphere in both panels.</p>", "links"=>[], "tags"=>["interactions", "residues"], "article_id"=>512781, "categories"=>["Infectious Diseases", "Developmental Biology", "Neuroscience"], "users"=>["Jorge E. Zuniga", "Jared T. Hammill", "Omri Drory", "Jonathan E. Nuss", "James C. Burnett", "Rick Gussio", "Peter Wipf", "Sina Bavari", "Axel T. Brunger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011378.g006", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Nonpolar_interactions_of_P2_8242_residues_of_PLMs_/512781", "title"=>"Nonpolar interactions of P2′ residues of PLMs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-06-30 00:46:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/841891"], "description"=>"<p><b>A.</b> View of the initial σ<sub>A</sub>-weighted F<sub>o</sub>-F<sub>c</sub> difference electron-density map contoured at 2.0 σ (grey mesh) around the inhibitor-binding site, and overlaid with the refined model of the complex (<b>JTH-NB72-39</b> is depicted in orange sticks, the Zn(II) atom as a yellow sphere, and the BoNT/A LC in cyan ribbon representation). The map was computed with phases calculated prior to the inclusion of <b>JTH-NB72-39</b> (i.e. it is a model-bias free map). For the PLM nitrogen, oxygen, and sulfur atoms are colored blue, red, and yellow, respectively. <b>B.</b> The same as A, but visualized from a different angle. <b>C.</b> Inhibiting interactions of <b>JTH-NB72-39</b>. BoNT/A LC residues are displayed as cyan sticks, and the <b>JTH-NB72-39</b> backbone is shown as thin, orange sticks. Only the P1′ Arg side chain of the inhibitor is shown as reference. Interactions between BoNT/A LC and <b>JTH-NB72-39</b> are represented by dashed lines. The identity of the residues is indicated. The P1 amino and carbonyl groups are indicated by NH<sub>2</sub> and CO, respectively. The C-terminus of the inhibitor is indicated by the letter C. The Zn(II) atom is represented as a yellow sphere.</p>", "links"=>[], "tags"=>["electron", "jth-nb72-39", "inhibitor", "inhibition", "lc"], "article_id"=>512328, "categories"=>["Infectious Diseases", "Developmental Biology", "Neuroscience"], "users"=>["Jorge E. Zuniga", "Jared T. Hammill", "Omri Drory", "Jonathan E. Nuss", "James C. Burnett", "Rick Gussio", "Peter Wipf", "Sina Bavari", "Axel T. Brunger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011378.g002", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Initial_electron_density_for_the_JTH_NB72_39_inhibitor_and_inhibition_of_the_BoNT_A_LC_8216_catalytic_engine_8217_/512328", "title"=>"Initial electron density for the JTH-NB72-39 inhibitor and inhibition of the BoNT/A LC ‘catalytic engine’.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-06-30 00:38:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/842216"], "description"=>"<p>Superposition of the structures of the BoNT/A LC complexes with inhibitors <b>JTH-NB72-39</b> (orange carbons) and: RRATKM (light blue carbons, panel <b>A</b>), <b>I1</b> (grey carbons, panel <b>B</b>), and CRATKML (light blue, panel <b>C</b>). BoNT/A LC residues are shown in cyan stick representation, with oxygen and nitrogen atoms colored red and blue, respectively. The Zn(II) ion and water molecule are displayed as yellow and blue spheres, respectively. The amino terminal groups of all inhibitors are labeled as NH<sub>2</sub>. Backbone amide groups in the P2′ position of <b>I1</b> and <b>JTH-NB72-39</b> are displayed as purple sticks. Dashed lines indicate intermolecular contacts between PLMs and the BoNT/A LC. <b>D.</b> Stick representation of the P2′ Phe and P5′ Met residues of the <b>JTH-NB72-39</b> inhibitor in its bound conformation. The intramolecular H-bond between the P2′ Phe carbonyl and the P5′ Met amide groups is indicated as a black dash line. <b>JTH-NB72-39</b> is displayed with orange carbons and ribbon; BoNT/A LC secondary structure and surface representation is colored cyan. Nitrogen, carbon, and sulfur atoms are blue, red and yellow, respectively. The Zn(II) atom is displayed as a yellow sphere.</p>", "links"=>[], "tags"=>["interactions"], "article_id"=>512660, "categories"=>["Infectious Diseases", "Developmental Biology", "Neuroscience"], "users"=>["Jorge E. Zuniga", "Jared T. Hammill", "Omri Drory", "Jonathan E. Nuss", "James C. Burnett", "Rick Gussio", "Peter Wipf", "Sina Bavari", "Axel T. Brunger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011378.g005", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Inhibitory_interactions_of_PLMs_/512660", "title"=>"Inhibitory interactions of PLMs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-06-30 00:44:20"}
  • {"files"=>["https://ndownloader.figshare.com/files/842789"], "description"=>"<p>*Abbreviations:</p><p>SecStr: observed secondary structure from pdb coordinates (L: loop; H: Helix; S: Bend).</p><p>α: helical chirality (dihedral α angle – positive angle corresponds to a right-handed helix).</p><p>RRGX: any of the tetrapeptides reported in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011378#pone.0011378-Kumaran3\" target=\"_blank\">[51]</a>.</p><p>All SS and α values were calculated with the DSSPcont program DSSPcont: Continuous secondary structure assignments for proteins <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011378#pone.0011378-Carter1\" target=\"_blank\">[61]</a>.</p>", "links"=>[], "tags"=>["lc", "plm"], "article_id"=>513233, "categories"=>["Infectious Diseases", "Developmental Biology", "Neuroscience"], "users"=>["Jorge E. Zuniga", "Jared T. Hammill", "Omri Drory", "Jonathan E. Nuss", "James C. Burnett", "Rick Gussio", "Peter Wipf", "Sina Bavari", "Axel T. Brunger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011378.t002", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Helicity_in_BoNT_A_LC_PLM_inhibitors_/513233", "title"=>"Helicity in BoNT/A LC PLM inhibitors.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2010-06-30 00:53:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/841999"], "description"=>"<p><b>A.</b> Panoramic view of <b>JTH-NB72-39</b> in the binding cleft of the BoNT/A LC. <b>JTH-NB72-39</b> is displayed in orange sticks (for selected residues only) and ribbon representation, with its directionality indicated by its N and C termini. <b>B.</b> Superposition of RRGI (purple), RRGL (pink), RRGM (magenta), RRGC (green), RRATKM (blue), and <b>I1</b> (grey) PLM inhibitors in the binding cleft of the BoNT/A LC. In both panels, selected BoNT/A LC residues are shown in cyan stick and surface representation, and the nitrogen and oxygen atoms of all inhibitors are colored blue and red, respectively. The coordinates for the BoNT/A LC are those of the <b>JTH-NB72-39</b>-bound (panel A) and the <b>I1</b>-bound (panel B) complexes. The Zn(II) atom is displayed as a yellow sphere in both panels. Negatively-charged patches in the BoNT/A LC surface involved in ionic contacts (black dashes) are displayed as red surface.</p>", "links"=>[], "tags"=>["binding", "jth-nb72-39", "lc", "substrate"], "article_id"=>512450, "categories"=>["Infectious Diseases", "Developmental Biology", "Neuroscience"], "users"=>["Jorge E. Zuniga", "Jared T. Hammill", "Omri Drory", "Jonathan E. Nuss", "James C. Burnett", "Rick Gussio", "Peter Wipf", "Sina Bavari", "Axel T. Brunger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011378.g003", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_binding_of_JTH_NB72_39_in_the_BoNT_A_LC_substrate_cleft_/512450", "title"=>"The binding of JTH-NB72-39 in the BoNT/A LC substrate cleft.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-06-30 00:40:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/841689"], "description"=>"<p>From top to bottom, schematic representation of the SNAP-25 P1-P6′ segment, with the substrate scissile bond indicated in red; representation of the <b>I1</b> and RRGC PLMs and their corresponding inhibition constants; schematic representation of the design strategy for the <b>JTH-NB72</b> PLMs, showing the incorporation of the Arg and the aromatic sidechain (R) in the P1 and the P2′ positions, respectively. The aromatic structures tested in the three <b>JTH-NB72</b> PLMs are indicated, together with their corresponding K<sub>i</sub> values and standard deviations (these values were calculated as the average of three different measurements).</p>", "links"=>[], "tags"=>["dermatology", "infectious diseases/bacterial infections", "neurological disorders/neuromuscular diseases"], "article_id"=>512120, "categories"=>["Infectious Diseases", "Developmental Biology", "Neuroscience"], "users"=>["Jorge E. Zuniga", "Jared T. Hammill", "Omri Drory", "Jonathan E. Nuss", "James C. Burnett", "Rick Gussio", "Peter Wipf", "Sina Bavari", "Axel T. Brunger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011378.g001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_PLM_structures_K_i_values_and_design_strategy_/512120", "title"=>"PLM structures, K<sub>i</sub> values, and design strategy.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-06-30 00:35:20"}
  • {"files"=>["https://ndownloader.figshare.com/files/842110"], "description"=>"<p>Close-up views of the P1 Arg residues of PLMs RRGI (purple carbons), RRGL (pink carbons), RRGM (magenta carbons), RRGC (pale green carbons), RRATKM (tan carbons), and <b>JTH-NB72-39</b> (orange carbons). BoNT/A LC structures in complex with the PLM inhibitors indicated above were superposed. BoNT/A LC residues are depicted in larger stick (with cyan carbons and backbone) and are taken from the coordinates of the BoNT/A LC:<b>JTH-NB72-39</b> complex. The Zn(II) ion is shown as a yellow sphere. Nitrogen and oxygen atoms are shown in blue and red, respectively.</p>", "links"=>[], "tags"=>["interactions", "p1", "residue"], "article_id"=>512558, "categories"=>["Infectious Diseases", "Developmental Biology", "Neuroscience"], "users"=>["Jorge E. Zuniga", "Jared T. Hammill", "Omri Drory", "Jonathan E. Nuss", "James C. Burnett", "Rick Gussio", "Peter Wipf", "Sina Bavari", "Axel T. Brunger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011378.g004", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Ionic_interactions_for_the_P1_residue_of_PLMs_/512558", "title"=>"Ionic interactions for the P1 residue of PLMs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-06-30 00:42:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/842740"], "description"=>"<p>X-ray data collection and refinement.</p>", "links"=>[], "tags"=>["dermatology", "infectious diseases/bacterial infections", "neurological disorders/neuromuscular diseases"], "article_id"=>513180, "categories"=>["Infectious Diseases", "Developmental Biology", "Neuroscience"], "users"=>["Jorge E. Zuniga", "Jared T. Hammill", "Omri Drory", "Jonathan E. Nuss", "James C. Burnett", "Rick Gussio", "Peter Wipf", "Sina Bavari", "Axel T. Brunger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011378.t001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_X_ray_data_collection_and_refinement_/513180", "title"=>"X-ray data collection and refinement.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2010-06-30 00:53:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/419519", "https://ndownloader.figshare.com/files/419562", "https://ndownloader.figshare.com/files/419599", "https://ndownloader.figshare.com/files/419632", "https://ndownloader.figshare.com/files/419677", "https://ndownloader.figshare.com/files/419717", "https://ndownloader.figshare.com/files/419762", "https://ndownloader.figshare.com/files/419792", "https://ndownloader.figshare.com/files/419820", "https://ndownloader.figshare.com/files/419860", "https://ndownloader.figshare.com/files/419928"], "description"=>"<div><p>The botulinum neurotoxin serotype A light chain (BoNT/A LC) protease is the catalytic component responsible for the neuroparalysis that is characteristic of the disease state botulism. Three related peptide-like molecules (PLMs) were designed using previous information from co-crystal structures, synthesized, and assayed for <em>in vitro</em> inhibition against BoNT/A LC. Our results indicate these PLMS are competitive inhibitors of the BoNT/A LC protease and their K<sub>i</sub> values are in the nM-range. A co-crystal structure for one of these inhibitors was determined and reveals that the PLM, in accord with the goals of our design strategy, simultaneously involves both ionic interactions via its P1 residue and hydrophobic contacts by means of an aromatic group in the P2′ position. The PLM adopts a helical conformation similar to previously determined co-crystal structures of PLMs, although there are also major differences to these other structures such as contacts with specific BoNT/A LC residues. Our structure further demonstrates the remarkable plasticity of the substrate binding cleft of the BoNT/A LC protease and provides a paradigm for iterative structure-based design and development of BoNT/A LC inhibitors.</p></div>", "links"=>[], "tags"=>["iterative", "structure-based", "peptide-like", "inhibitor", "botulinum", "neurotoxin", "serotype"], "article_id"=>142841, "categories"=>["Cancer", "Developmental Biology", "Neuroscience"], "users"=>["Jorge E. Zuniga", "Jared T. Hammill", "Omri Drory", "Jonathan E. Nuss", "James C. Burnett", "Rick Gussio", "Peter Wipf", "Sina Bavari", "Axel T. Brunger"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0011378.s001", "https://dx.doi.org/10.1371/journal.pone.0011378.s002", "https://dx.doi.org/10.1371/journal.pone.0011378.s003", "https://dx.doi.org/10.1371/journal.pone.0011378.s004", "https://dx.doi.org/10.1371/journal.pone.0011378.s005", "https://dx.doi.org/10.1371/journal.pone.0011378.s006", "https://dx.doi.org/10.1371/journal.pone.0011378.s007", "https://dx.doi.org/10.1371/journal.pone.0011378.s008", "https://dx.doi.org/10.1371/journal.pone.0011378.s009", "https://dx.doi.org/10.1371/journal.pone.0011378.s010", "https://dx.doi.org/10.1371/journal.pone.0011378.s011"], "stats"=>{"downloads"=>26, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Iterative_Structure_Based_Peptide_Like_Inhibitor_Design_against_the_Botulinum_Neurotoxin_Serotype_A/142841", "title"=>"Iterative Structure-Based Peptide-Like Inhibitor Design against the Botulinum Neurotoxin Serotype A", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2010-06-30 00:47:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/842436"], "description"=>"<p>Superposition of: <b>A.</b> The 70 loop backbone of BoNT/A LC in the unbound (tan), <b>I1</b>- (blue), <b>JTH-NB72-39</b>- (cyan), ArgHX- (red), and CRATKML-bound forms (dark green). <b>B.</b> The 70 loop backbone of the BoNT/A LC in the unbound (tan), <b>I1</b>- (blue), <b>JTH-NB72-39</b>- (cyan), RRGM- (magenta), and RRATKM-bound (orange) forms. <b>C.</b> The 250 loop backbone of the BoNT/A LC in the unbound (tan), <b>I1</b>- (blue), <b>JTH-NB72-39</b>- (cyan), ArgHX- (red), and QRATKM- bound (grey) forms. <b>D.</b> The 370 loop backbone of the BoNT/A LC in the unbound (tan), <b>JTH-NB72-39</b>- (red), <b>I1</b>- (blue), and ArgHX-bound (cyan). The backbone is displayed in cartoon representation and the side chain of BoNT/A LC Phe 369 is shown as sticks.</p>", "links"=>[], "tags"=>["plasticity", "lc", "substrate", "binding"], "article_id"=>512874, "categories"=>["Infectious Diseases", "Developmental Biology", "Neuroscience"], "users"=>["Jorge E. Zuniga", "Jared T. Hammill", "Omri Drory", "Jonathan E. Nuss", "James C. Burnett", "Rick Gussio", "Peter Wipf", "Sina Bavari", "Axel T. Brunger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011378.g007", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Observed_plasticity_in_the_BoNT_A_LC_substrate_binding_cleft_/512874", "title"=>"Observed plasticity in the BoNT/A LC substrate binding cleft.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-06-30 00:47:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/842641"], "description"=>"<p><b>A.</b> Cartoon representation of the BoNT/A LC:<b>I1</b> complex. The BoNT/A LC is shown in blue, and <b>I1</b> in dark grey. The dashed line represents an H-bond between the indole nitrogen atom of the P2′ Trp and the carbonyl group of BoNT/A LC residue Glu 257. The H-bond distance is indicated. <b>B.</b> Cartoon representation of the BoNT/A LC:<b>JTH-NB72-39</b> complex. A Trp side chain has been modeled in the P2′ position (instead of the actual Phe side chain of <b>JTH-NB72-39</b>). The Trp rotamer model shown here positions the indole nitrogen as can bind in the closest possible proximity to the carbonyl group in Glu 257 (employing the BoNT/A LC: <b>JTH NB72-39</b> co-crystal). The solid line indicates the distance (not contact) between these two groups. The BoNT/A LC is shown in cyan, and <b>JTH-NB72-39</b> carbons and backbone ribbon in orange. The side chains of the P2′ Trp in the PLMs and Glu 257 in BoNT/A LC are displayed in stick representation in both panels. The yellow spheres represent the Zn(II) ion in both panels.</p>", "links"=>[], "tags"=>["trp", "residue"], "article_id"=>513080, "categories"=>["Infectious Diseases", "Developmental Biology", "Neuroscience"], "users"=>["Jorge E. Zuniga", "Jared T. Hammill", "Omri Drory", "Jonathan E. Nuss", "James C. Burnett", "Rick Gussio", "Peter Wipf", "Sina Bavari", "Axel T. Brunger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011378.g009", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Modeling_of_a_Trp_residue_in_the_P2_8242_position_of_JTH_NB72_39_/513080", "title"=>"Modeling of a Trp residue in the P2′ position of JTH-NB72-39.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-06-30 00:51:20"}
  • {"files"=>["https://ndownloader.figshare.com/files/842549"], "description"=>"<p>Superposition of the backbones (tube representation) of all BoNT/A LC PLM inhibitors reported to date (and which are longer than four residues). <b>A</b>. PLM inhibitors whose backbones display positive helical chirality upon binding to BoNT/A LC: <b>JTH-NB72-39</b> (orange), <b>I1</b> (grey), and CRATKML (blue). <b>B</b>. PLM inhibitors with negative helical chirality upon binding to BoNT/A LC: RRATKM (green), and QRATKM (red). <b>JTH-NB72-39</b> (orange) is shown for comparative purposes. The BoNT/A LC Zn(II) ion is shown as a yellow sphere.</p>", "links"=>[], "tags"=>["helical", "chirality", "plm"], "article_id"=>512996, "categories"=>["Infectious Diseases", "Developmental Biology", "Neuroscience"], "users"=>["Jorge E. Zuniga", "Jared T. Hammill", "Omri Drory", "Jonathan E. Nuss", "James C. Burnett", "Rick Gussio", "Peter Wipf", "Sina Bavari", "Axel T. Brunger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011378.g008", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_helical_chirality_of_PLM_inhibitors_/512996", "title"=>"The helical chirality of PLM inhibitors.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-06-30 00:49:56"}

PMC Usage Stats | Further Information

  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"4", "full-text"=>"26", "unique-ip"=>"30", "pdf"=>"19", "year"=>"2010", "figure"=>"11", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"34", "year"=>"2010", "pdf"=>"26", "unique-ip"=>"33", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"33", "unique-ip"=>"36", "pdf"=>"19", "year"=>"2010", "figure"=>"9", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"13", "year"=>"2010", "pdf"=>"10", "unique-ip"=>"17", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"23", "unique-ip"=>"22", "pdf"=>"13", "year"=>"2010", "figure"=>"9", "scanned-summary"=>"0", "supp-data"=>"11"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"17", "year"=>"2010", "pdf"=>"11", "unique-ip"=>"16", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"7"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"6", "unique-ip"=>"6", "pdf"=>"4", "year"=>"2011", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"5", "year"=>"2011", "pdf"=>"3", "unique-ip"=>"11", "figure"=>"10", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"10", "unique-ip"=>"13", "pdf"=>"5", "year"=>"2011", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"15", "year"=>"2011", "pdf"=>"7", "unique-ip"=>"19", "figure"=>"16", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"7", "unique-ip"=>"7", "pdf"=>"5", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"9", "year"=>"2011", "pdf"=>"4", "unique-ip"=>"10", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"3", "unique-ip"=>"2", "pdf"=>"1", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"6", "year"=>"2011", "pdf"=>"4", "unique-ip"=>"8", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"4", "unique-ip"=>"4", "pdf"=>"4", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"2", "year"=>"2011", "pdf"=>"1", "unique-ip"=>"3", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"6", "unique-ip"=>"6", "pdf"=>"4", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"2", "year"=>"2011", "pdf"=>"3", "unique-ip"=>"4", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"13", "unique-ip"=>"8", "pdf"=>"6", "year"=>"2012", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"6", "year"=>"2012", "pdf"=>"3", "unique-ip"=>"8", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"4", "unique-ip"=>"5", "pdf"=>"1", "year"=>"2012", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"1", "year"=>"2012", "pdf"=>"0", "unique-ip"=>"2", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"0", "unique-ip"=>"1", "pdf"=>"0", "year"=>"2012", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"0", "year"=>"2012", "pdf"=>"2", "unique-ip"=>"2", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"13", "pdf"=>"1", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"11", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"11", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"3", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"26", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"15", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"1", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"2", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"16", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"11", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"13", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}

Relative Metric

{"start_date"=>"2010-01-01T00:00:00Z", "end_date"=>"2010-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[288, 576, 733, 867, 984, 1087, 1182, 1267, 1346, 1424, 1501, 1577, 1646, 1711, 1778, 1841, 1908, 1970, 2034, 2102, 2162, 2227, 2296, 2359, 2422, 2482, 2550, 2610, 2679, 2747, 2820, 2887, 2955, 3009, 3067, 3130, 3200, 3257, 3322, 3379, 3443, 3507, 3571, 3632, 3683, 3753, 3822, 3877]}, {"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[283, 565, 720, 852, 970, 1070, 1168, 1258, 1336, 1411, 1487, 1571, 1640, 1709, 1776, 1843, 1906, 1969, 2031, 2086, 2152, 2221, 2286, 2361, 2419, 2480, 2543, 2598, 2665, 2731, 2802, 2868, 2932, 2996, 3053, 3114, 3169, 3232, 3289, 3355, 3413, 3473, 3530, 3595, 3648, 3711, 3764, 3810, 3863]}, {"subject_area"=>"/Medicine and health sciences/Pathology and laboratory medicine", "average_usage"=>[274, 546, 703, 837, 951, 1058, 1152, 1234, 1323, 1387, 1453, 1538, 1606, 1694, 1753, 1814, 1873, 1941, 2011, 2079, 2137, 2193, 2260, 2325, 2386, 2459, 2528, 2598, 2651, 2728, 2788, 2859, 2931, 2999, 3068, 3131, 3209, 3258, 3321, 3372, 3426, 3500, 3561, 3613, 3685, 3743, 3805, 3839]}, {"subject_area"=>"/Physical sciences/Physics", "average_usage"=>[280, 523, 669, 776, 867, 950, 1024, 1087, 1147, 1208, 1263, 1323, 1373, 1436, 1480, 1535, 1589, 1652, 1703, 1744, 1796, 1849, 1910, 1970, 2017, 2064, 2113, 2173, 2225, 2280, 2332, 2381, 2457, 2506, 2573, 2630, 2678, 2735, 2792, 2831, 2892, 2946, 2992, 3041, 3101, 3148, 3195, 3285, 3326]}]}
Loading … Spinner
There are currently no alerts