Gene Expression Analysis of Forskolin Treated Basilar Papillae Identifies MicroRNA181a as a Mediator of Proliferation
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Gene expression analysis of forskolin treated basilar papillae identifies microRNA181a as a mediator of proliferation", "type"=>"journal", "authors"=>[{"first_name"=>"Corey S.", "last_name"=>"Frucht", "scopus_author_id"=>"36607844600"}, {"first_name"=>"Mohamed", "last_name"=>"Uduman", "scopus_author_id"=>"24170246400"}, {"first_name"=>"Jamie L.", "last_name"=>"Duke", "scopus_author_id"=>"36058814300"}, {"first_name"=>"Steven H.", "last_name"=>"Kleinstein", "scopus_author_id"=>"6506423505"}, {"first_name"=>"Joseph", "last_name"=>"Santos-Sacchi", "scopus_author_id"=>"7004191223"}, {"first_name"=>"Dhasakumar S.", "last_name"=>"Navaratnam", "scopus_author_id"=>"35579890400"}], "year"=>2010, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"20634979", "sgr"=>"77955455031", "doi"=>"10.1371/journal.pone.0011502", "scopus"=>"2-s2.0-77955455031", "pui"=>"359322485", "isbn"=>"1932-6203 (Electronic)\r1932-6203 (Linking)", "issn"=>"19326203"}, "id"=>"ed4c911d-954f-3dd3-8340-653d4ee431dc", "abstract"=>"BACKGROUND: Auditory hair cells spontaneously regenerate following injury in birds but not mammals. A better understanding of the molecular events underlying hair cell regeneration in birds may allow for identification and eventually manipulation of relevant pathways in mammals to stimulate regeneration and restore hearing in deaf patients.\\n\\nMETHODOLOGY/PRINCIPAL FINDINGS: Gene expression was profiled in forskolin treated (i.e., proliferating) and quiescent control auditory epithelia of post-hatch chicks using an Affymetrix whole-genome chicken array after 24 (n = 6), 48 (n = 6), and 72 (n = 12) hours in culture. In the forskolin-treated epithelia there was significant (p<0.05; >two-fold change) upregulation of many genes thought to be relevant to cell cycle control and inner ear development. Gene set enrichment analysis was performed on the data and identified myriad microRNAs that are likely to be upregulated in the regenerating tissue, including microRNA181a (miR181a), which is known to mediate proliferation in other systems. Functional experiments showed that miR181a overexpression is sufficient to stimulate proliferation within the basilar papilla, as assayed by BrdU incorporation. Further, some of the newly produced cells express the early hair cell marker myosin VI, suggesting that miR181a transfection can result in the production of new hair cells.\\n\\nCONCLUSIONS/SIGNIFICANCE: These studies have identified a single microRNA, miR181a, that can cause proliferation in the chicken auditory epithelium with production of new hair cells.", "link"=>"http://www.mendeley.com/research/gene-expression-analysis-forskolin-treated-basilar-papillae-identifies-microrna181a-mediator-prolife", "reader_count"=>29, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>2, "Researcher"=>15, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>4, "Student > Postgraduate"=>1, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>1, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>2, "Researcher"=>15, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>4, "Student > Postgraduate"=>1, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>1, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>6, "Agricultural and Biological Sciences"=>11, "Medicine and Dentistry"=>5, "Neuroscience"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Veterinary Science and Veterinary Medicine"=>1, "Chemistry"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>5}, "Neuroscience"=>{"Neuroscience"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>11}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}, "Unspecified"=>{"Unspecified"=>2}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "reader_count_by_country"=>{"South Africa"=>1, "Chile"=>1}, "group_count"=>1}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/77955455031"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/77955455031?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77955455031&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=77955455031&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/77955455031", "dc:identifier"=>"SCOPUS_ID:77955455031", "eid"=>"2-s2.0-77955455031", "dc:title"=>"Gene expression analysis of forskolin treated basilar papillae identifies microRNA181a as a mediator of proliferation", "dc:creator"=>"Frucht C.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"5", "prism:issueIdentifier"=>"7", "prism:pageRange"=>nil, "prism:coverDate"=>"2010-08-16", "prism:coverDisplayDate"=>"2010", "prism:doi"=>"10.1371/journal.pone.0011502", "citedby-count"=>"15", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Yale University School of Medicine", "affiliation-city"=>"New Haven", "affiliation-country"=>"United States"}, {"@_fa"=>"true", "affilname"=>"Yale University", "affiliation-city"=>"New Haven", "affiliation-country"=>"United States"}], "pubmed-id"=>"20634979", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e11502", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0011502", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"7", "year"=>"2010", "pdf_views"=>"70", "xml_views"=>"11", "html_views"=>"243"}
  • {"month"=>"8", "year"=>"2010", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"97"}
  • {"month"=>"9", "year"=>"2010", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"72"}
  • {"month"=>"10", "year"=>"2010", "pdf_views"=>"21", "xml_views"=>"2", "html_views"=>"73"}
  • {"month"=>"11", "year"=>"2010", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"12", "year"=>"2010", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"48"}
  • {"month"=>"1", "year"=>"2011", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"35"}
  • {"month"=>"2", "year"=>"2011", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"3", "year"=>"2011", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"87"}
  • {"month"=>"4", "year"=>"2011", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"5", "year"=>"2011", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"6", "year"=>"2011", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"7", "year"=>"2011", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"8", "year"=>"2011", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"9", "year"=>"2011", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"10", "year"=>"2011", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"11", "year"=>"2011", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"34"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"5", "html_views"=>"25"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"34"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"29"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"0", "xml_views"=>"2", "html_views"=>"33"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"25"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"0", "xml_views"=>"2", "html_views"=>"21"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"84"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"11"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"4", "html_views"=>"0"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"3"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/841301"], "description"=>"<p>Shown is a heat map depicting fold-changes between the forskolin and control conditions for a set of genes of interest the three time points indicated. The genes shown were selected because of their known expression in the inner ear (i.e., RARB, CALB1, OCM), ion channel identity (e.g., KCNAB1, KCNJ15), or relevance to the present study (i.e., E2F1). The genes span a range of fold-changes and are both up and downregulated with forskolin treatment. Fold-changes are given as log<sub>2</sub>(expression in forskolin/expression in control). The directionality of fold-changes was fairly consistent across time points, but there was a slight general trend toward increasing fold-change values from 24 to 72 hours.</p>", "links"=>[], "tags"=>["differences", "genes", "72"], "article_id"=>511757, "categories"=>["Neuroscience", "Medicine"], "users"=>["Corey S. Frucht", "Mohamed Uduman", "Jamie L. Duke", "Steven H. Kleinstein", "Joseph Santos-Sacchi", "Dhasakumar S. Navaratnam"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011502.g002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Fold_change_differences_of_selected_genes_of_interest_remain_stable_at_24_48_and_72_hours_/511757", "title"=>"Fold-change differences of selected genes of interest remain stable at 24, 48, and 72 hours.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-07-09 00:29:17"}
  • {"files"=>["https://ndownloader.figshare.com/files/841594"], "description"=>"<p>Shown is a confocal image of a basilar papillae that was transfected with pre-miR181a, cultured for 72 hours, then cryosectioned and labeled for BrdU (blue) and the early hair cell marker myosin VI (red) (<b>A</b>). The apical surface of hair cells is to the left of the image and the neural edge of the basilar papillae is toward the bottom. The tectorial membrane has been removed to allow for antibody labeling. The inset of <b>A</b> is also shown in panels <b>B</b> (myosin VI), <b>C</b> (BrdU) and <b>D</b> (merge). The arrow in <b>D</b> shows a cell that expresss myosin VI and has a BrdU positive nucleus, whereas the arrowhead shows a cell that is labeling for myosin VI but not BrdU. The double-labeled cell is one that has been stimulated to divide and subsequently begin differentiating toward a hair cell phenotype. The scale bar in <b>B</b> also applies to <b>C</b> and <b>D</b>.</p>", "links"=>[], "tags"=>["cells", "produced", "mir181a", "transfection", "myosin"], "article_id"=>512044, "categories"=>["Neuroscience", "Medicine"], "users"=>["Corey S. Frucht", "Mohamed Uduman", "Jamie L. Duke", "Steven H. Kleinstein", "Joseph Santos-Sacchi", "Dhasakumar S. Navaratnam"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011502.g004", "stats"=>{"downloads"=>1, "page_views"=>73, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Some_new_cells_produced_by_miR181a_transfection_express_the_early_hair_cell_marker_myosin_VI_/512044", "title"=>"Some new cells produced by miR181a transfection express the early hair cell marker myosin VI.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-07-09 00:34:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/841853"], "description"=>"<p>The number of mammalian miRNA/transcription factor motif based (“Mammalian”), chicken/frog conservation transcription factor motif based gene sets (“Conservation”), and curated gene sets from the BROAD Institute (“Curated”) meeting the criteria <i>p</i><0.05 and <i>q</i> (false discovery rate) <0.25 for forskolin (FSK) and control (CTL) samples after 24, 48, or 72 hours in culture.</p>", "links"=>[], "tags"=>["enrichment"], "article_id"=>512314, "categories"=>["Neuroscience", "Medicine"], "users"=>["Corey S. Frucht", "Mohamed Uduman", "Jamie L. Duke", "Steven H. Kleinstein", "Joseph Santos-Sacchi", "Dhasakumar S. Navaratnam"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011502.t001", "stats"=>{"downloads"=>3, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Gene_set_enrichment_analysis_results_/512314", "title"=>"Gene set enrichment analysis results.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2010-07-09 00:38:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/841788"], "description"=>"<p>QPCR and microarray data for individual genes of interest and genes selected for qPCR validation as described in the text. Multiple microarray values represent different probe sets for the same gene.</p>", "links"=>[], "tags"=>["validation"], "article_id"=>512244, "categories"=>["Neuroscience", "Medicine"], "users"=>["Corey S. Frucht", "Mohamed Uduman", "Jamie L. Duke", "Steven H. Kleinstein", "Joseph Santos-Sacchi", "Dhasakumar S. Navaratnam"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011502.t003", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_QPCR_validation_of_gene_expression_data_/512244", "title"=>"QPCR validation of gene expression data.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2010-07-09 00:37:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/841720"], "description"=>"<p>Shown are the mammalian gene set enrichment analysis results for selected gene sets of interest at 24, 48, and 72 hours. Italicized are those gene sets with p-value <0.05 and false discovery rate (FDR) <0.25, which are taken as significant. Gene sets are directly as they appear in the Molecular Signatures Database on the Broad Institute website.</p>", "links"=>[], "tags"=>["enrichment"], "article_id"=>512177, "categories"=>["Neuroscience", "Medicine"], "users"=>["Corey S. Frucht", "Mohamed Uduman", "Jamie L. Duke", "Steven H. Kleinstein", "Joseph Santos-Sacchi", "Dhasakumar S. Navaratnam"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011502.t005", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mammalian_gene_set_enrichment_analysis_results_/512177", "title"=>"Mammalian gene set enrichment analysis results.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2010-07-09 00:36:17"}
  • {"files"=>["https://ndownloader.figshare.com/files/841206"], "description"=>"<p>Shown is a proportional Venn diagram indicating the number of genes significantly (p<0.05 and fold change >2) differentially expressed at 24, 48, and 72 hours of exposure to forskolin. The number of genes in each category is indicated in parentheses. Far more genes are affected at 72 hours than at either of the two earlier time points. A little over half of the genes differentially expressed at 24 or 48 hours are also differentially expressed at 72 hours. Only one gene, AMPH, was affected at all three time points.</p>", "links"=>[], "tags"=>["genes", "differentially", "72", "24", "48"], "article_id"=>511650, "categories"=>["Neuroscience", "Medicine"], "users"=>["Corey S. Frucht", "Mohamed Uduman", "Jamie L. Duke", "Steven H. Kleinstein", "Joseph Santos-Sacchi", "Dhasakumar S. Navaratnam"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011502.g001", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Far_more_genes_were_differentially_expressed_at_72_hours_than_at_24_or_48_hours_of_exposure_to_forskolin_/511650", "title"=>"Far more genes were differentially expressed at 72 hours than at 24 or 48 hours of exposure to forskolin.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-07-09 00:27:30"}
  • {"files"=>["https://ndownloader.figshare.com/files/841682"], "description"=>"<p>Shown are the results of the conservation gene set enrichment analysis performed on gene expression data from 24, 48, and 72 hours in culture. These analyses were performed using gene sets based on transcription factor motifs in regions conserved between the chicken and frog genomes. The gene sets shown here were downloaded from the BROAD Institute website and represent empirically defined sets of genes. References for each gene set are provided in the text. Italicized are those gene sets with p-value <0.05 and false discovery rate (FDR) <0.25, which are taken as significant. Gene sets are directly as they appear in the Molecular Signatures Database on the Broad Institute website.</p>", "links"=>[], "tags"=>["enrichment"], "article_id"=>512143, "categories"=>["Neuroscience", "Medicine"], "users"=>["Corey S. Frucht", "Mohamed Uduman", "Jamie L. Duke", "Steven H. Kleinstein", "Joseph Santos-Sacchi", "Dhasakumar S. Navaratnam"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011502.t006", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Conservation_gene_set_enrichment_analysis_results_/512143", "title"=>"Conservation gene set enrichment analysis results.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2010-07-09 00:35:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/841748"], "description"=>"<p>Shown are the curated gene set enrichment analysis results for selected gene sets of interest at 24, 48, and 72 hours. The gene sets shown here were downloaded from the BROAD Institute website and represent empirically defined sets of genes. References for each gene set are provided in the text. Italicized are those gene sets with p-value <0.05 and false discovery rate (FDR) <0.25, which are taken as significant. Gene sets are directly as they appear in the Molecular Signatures Database on the Broad Institute website.</p>", "links"=>[], "tags"=>["enrichment"], "article_id"=>512208, "categories"=>["Neuroscience", "Medicine"], "users"=>["Corey S. Frucht", "Mohamed Uduman", "Jamie L. Duke", "Steven H. Kleinstein", "Joseph Santos-Sacchi", "Dhasakumar S. Navaratnam"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011502.t004", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Curated_gene_set_enrichment_analysis_results_/512208", "title"=>"Curated gene set enrichment analysis results.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2010-07-09 00:36:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/418880", "https://ndownloader.figshare.com/files/418892", "https://ndownloader.figshare.com/files/418904", "https://ndownloader.figshare.com/files/418923", "https://ndownloader.figshare.com/files/418938", "https://ndownloader.figshare.com/files/418958", "https://ndownloader.figshare.com/files/418976", "https://ndownloader.figshare.com/files/418996", "https://ndownloader.figshare.com/files/419006", "https://ndownloader.figshare.com/files/419026", "https://ndownloader.figshare.com/files/419050", "https://ndownloader.figshare.com/files/419065"], "description"=>"<div><h3>Background</h3><p>Auditory hair cells spontaneously regenerate following injury in birds but not mammals. A better understanding of the molecular events underlying hair cell regeneration in birds may allow for identification and eventually manipulation of relevant pathways in mammals to stimulate regeneration and restore hearing in deaf patients.</p><h3>Methodology/Principal Findings</h3><p>Gene expression was profiled in forskolin treated (i.e., proliferating) and quiescent control auditory epithelia of post-hatch chicks using an Affymetrix whole-genome chicken array after 24 (n = 6), 48 (n = 6), and 72 (n = 12) hours in culture. In the forskolin-treated epithelia there was significant (p<0.05; >two-fold change) upregulation of many genes thought to be relevant to cell cycle control and inner ear development. Gene set enrichment analysis was performed on the data and identified myriad microRNAs that are likely to be upregulated in the regenerating tissue, including microRNA181a (miR181a), which is known to mediate proliferation in other systems. Functional experiments showed that miR181a overexpression is sufficient to stimulate proliferation within the basilar papilla, as assayed by BrdU incorporation. Further, some of the newly produced cells express the early hair cell marker myosin VI, suggesting that miR181a transfection can result in the production of new hair cells.</p><h3>Conclusions/Significance</h3><p>These studies have identified a single microRNA, miR181a, that can cause proliferation in the chicken auditory epithelium with production of new hair cells.</p></div>", "links"=>[], "tags"=>["forskolin", "treated", "basilar", "papillae", "identifies", "microrna181a", "mediator", "proliferation"], "article_id"=>142724, "categories"=>["Neuroscience", "Medicine"], "users"=>["Corey S. Frucht", "Mohamed Uduman", "Jamie L. Duke", "Steven H. Kleinstein", "Joseph Santos-Sacchi", "Dhasakumar S. Navaratnam"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0011502.s001", "https://dx.doi.org/10.1371/journal.pone.0011502.s002", "https://dx.doi.org/10.1371/journal.pone.0011502.s003", "https://dx.doi.org/10.1371/journal.pone.0011502.s004", "https://dx.doi.org/10.1371/journal.pone.0011502.s005", "https://dx.doi.org/10.1371/journal.pone.0011502.s006", "https://dx.doi.org/10.1371/journal.pone.0011502.s007", "https://dx.doi.org/10.1371/journal.pone.0011502.s008", "https://dx.doi.org/10.1371/journal.pone.0011502.s009", "https://dx.doi.org/10.1371/journal.pone.0011502.s010", "https://dx.doi.org/10.1371/journal.pone.0011502.s011", "https://dx.doi.org/10.1371/journal.pone.0011502.s012"], "stats"=>{"downloads"=>15, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Gene_Expression_Analysis_of_Forskolin_Treated_Basilar_Papillae_Identifies_MicroRNA181a_as_a_Mediator_of_Proliferation/142724", "title"=>"Gene Expression Analysis of Forskolin Treated Basilar Papillae Identifies MicroRNA181a as a Mediator of Proliferation", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2010-07-09 00:45:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/841824"], "description"=>"<p><i><sup>a</sup></i>The entire gene expression data set was filtered to exclude probes whose expression was not at least 20% in at least one out of the six 24-hour samples (<b>24 H</b>), six 48-hour samples (<b>48 H</b>), or twelve 72-hour samples (<b>72 H</b>). The statistics reported are based only on those probes that met this criterion. Half of the samples from each time point were treated with forskolin (F) and the remainder served as controls (C). Showed in boldface are the statistics for the list of genes defined to be differentially expressed using p<0.05 and fold-change >2.</p>", "links"=>[], "tags"=>["otolaryngology", "neuroscience/sensory systems", "neurological disorders/neuro-ophthalmology and neuro-otology", "otolaryngology/audiology", "otolaryngology/ear pathologies"], "article_id"=>512274, "categories"=>["Neuroscience", "Medicine"], "users"=>["Corey S. Frucht", "Mohamed Uduman", "Jamie L. Duke", "Steven H. Kleinstein", "Joseph Santos-Sacchi", "Dhasakumar S. Navaratnam"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011502.t002", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Microarray_analysis_statistics_/512274", "title"=>"Microarray analysis statistics.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2010-07-09 00:37:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/841449"], "description"=>"<p>Basilar papillae from 0-day-old chicks were cultured for 72 hours in the presence of BrdU, the nuclear incorporation of which was detected by antibody labeling. In A–E sensory epithelia are outlined with dashed white lines. The neural edge of each epithelium is at the bottom, the abneural edge is at the top, the distal end is to the right and the proximal end is to the left of the image. BrdU-positive (i.e., recently divided) cells are immunohistochemically labeled and appear as purple dots. Individual cochleas were treated with 100 nM pre-miR181a (A), 100 µM forskolin (FSK) (B), FSK + 100 nM pre-miR181a (C), 100 µM FSK+ 100 nM anti-miR181a (D), or DMSO as a vector control (E). A pro-proliferative effect of miR181a can be appreciated. Further, knocking down endogenous miR181a appears to have a large suppressive effect on forskolin induced proliferation. Transfection was achieved in the first 24 hours of culture using X-tremeGENE siRNA Transfection Reagent in accordance with the manufacturer's instructions (Roche, Indianapolis, IN). The miRNA containing media was removed after 24 hours and replaced with normal medium. The summary of the average number of BrdU positive cells for the control (n = 7), miR181a (n = 8), forskolin (n = 8), forskolin + miR181a (n = 8), and forskolin + anti-miR181a (n = 3) conditions. The following comparisons were all statistically significant: control versus miR181a (p = 0.001), control versus forskolin (p<0.001), miR181a versus forskolin (p<0.001), forskolin versus forskolin plus anti-miR181a (p = 0.008) and forskolin versus forskolin plus miR181a (p = 0.005). Scale bars in panels A–E = 0.2 mm.</p>", "links"=>[], "tags"=>["overexpression", "produces", "proliferation", "basilar"], "article_id"=>511903, "categories"=>["Neuroscience", "Medicine"], "users"=>["Corey S. Frucht", "Mohamed Uduman", "Jamie L. Duke", "Steven H. Kleinstein", "Joseph Santos-Sacchi", "Dhasakumar S. Navaratnam"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0011502.g003", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_MicroRNA181a_overexpression_produces_proliferation_in_the_chicken_basilar_papilla_/511903", "title"=>"MicroRNA181a overexpression produces proliferation in the chicken basilar papilla.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-07-09 00:31:43"}

PMC Usage Stats | Further Information

  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"51", "unique-ip"=>"50", "pdf"=>"28", "year"=>"2010", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"5", "full-text"=>"45", "year"=>"2010", "pdf"=>"27", "unique-ip"=>"50", "figure"=>"6", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"37", "unique-ip"=>"40", "pdf"=>"20", "year"=>"2010", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"18", "year"=>"2010", "pdf"=>"15", "unique-ip"=>"21", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"27", "unique-ip"=>"31", "pdf"=>"15", "year"=>"2010", "figure"=>"6", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"32", "year"=>"2010", "pdf"=>"22", "unique-ip"=>"31", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"28", "unique-ip"=>"26", "pdf"=>"12", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"30", "year"=>"2011", "pdf"=>"17", "unique-ip"=>"31", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"4", "full-text"=>"18", "unique-ip"=>"22", "pdf"=>"11", "year"=>"2011", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"19", "year"=>"2011", "pdf"=>"13", "unique-ip"=>"18", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"17", "unique-ip"=>"22", "pdf"=>"8", "year"=>"2011", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"6"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"16", "year"=>"2011", "pdf"=>"6", "unique-ip"=>"16", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"15", "unique-ip"=>"15", "pdf"=>"12", "year"=>"2011", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"14", "year"=>"2011", "pdf"=>"3", "unique-ip"=>"13", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"7", "unique-ip"=>"8", "pdf"=>"5", "year"=>"2011", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"18", "year"=>"2011", "pdf"=>"12", "unique-ip"=>"18", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"9", "unique-ip"=>"10", "pdf"=>"6", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"5", "year"=>"2011", "pdf"=>"1", "unique-ip"=>"5", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"5", "unique-ip"=>"7", "pdf"=>"5", "year"=>"2012", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"6", "year"=>"2012", "pdf"=>"1", "unique-ip"=>"6", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"11", "unique-ip"=>"13", "pdf"=>"1", "year"=>"2012", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"5", "year"=>"2012", "pdf"=>"2", "unique-ip"=>"7", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"8", "unique-ip"=>"9", "pdf"=>"4", "year"=>"2012", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"5", "year"=>"2012", "pdf"=>"2", "unique-ip"=>"5", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"2", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"17", "full-text"=>"19", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"0", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"29", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"11", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"20", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"11", "cited-by"=>"1", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"0", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"11", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"16", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}

Relative Metric

{"start_date"=>"2010-01-01T00:00:00Z", "end_date"=>"2010-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[288, 576, 733, 867, 984, 1087, 1182, 1267, 1346, 1424, 1501, 1577, 1646, 1711, 1778, 1841, 1908, 1970, 2034, 2102, 2162, 2227, 2296, 2359, 2422, 2482, 2550, 2610, 2679, 2747, 2820, 2887, 2955, 3009, 3067, 3130, 3200, 3257, 3322, 3379, 3443, 3507, 3571, 3632, 3683, 3753, 3822, 3877]}, {"subject_area"=>"/Biology and life sciences/Agriculture", "average_usage"=>[289, 556, 715, 853, 962, 1053, 1146, 1230, 1307, 1373, 1443, 1524, 1582, 1669, 1720, 1810, 1876, 1929, 2012, 2087, 2168, 2241, 2320, 2385, 2469, 2534, 2605, 2681, 2745, 2812, 2861, 2909, 2988, 3068, 3166, 3213, 3266, 3346, 3419, 3508, 3566, 3631, 3692, 3746, 3809, 3882, 3964, 4024, 4100]}, {"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[283, 565, 720, 852, 970, 1070, 1168, 1258, 1336, 1411, 1487, 1571, 1640, 1709, 1776, 1843, 1906, 1969, 2031, 2086, 2152, 2221, 2286, 2361, 2419, 2480, 2543, 2598, 2665, 2731, 2802, 2868, 2932, 2996, 3053, 3114, 3169, 3232, 3289, 3355, 3413, 3473, 3530, 3595, 3648, 3711, 3764, 3810, 3863]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[306, 610, 768, 915, 1043, 1164, 1255, 1349, 1432, 1511, 1592, 1674, 1744, 1817, 1889, 1950, 2028, 2087, 2153, 2217, 2274, 2349, 2418, 2488, 2558, 2616, 2686, 2745, 2809, 2869, 2938, 3008, 3071, 3131, 3189, 3258, 3320, 3389, 3446, 3503, 3552, 3613, 3677, 3744, 3788, 3842, 3907, 3966, 4015]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[277, 558, 715, 845, 963, 1070, 1166, 1258, 1336, 1410, 1485, 1561, 1638, 1707, 1767, 1834, 1896, 1960, 2030, 2096, 2165, 2229, 2300, 2368, 2425, 2489, 2553, 2617, 2679, 2743, 2814, 2873, 2941, 2995, 3050, 3111, 3169, 3235, 3300, 3363, 3428, 3487, 3548, 3605, 3670, 3726, 3785, 3844]}, {"subject_area"=>"/Medicine and health sciences/Anatomy", "average_usage"=>[268, 527, 681, 804, 923, 1031, 1126, 1210, 1284, 1361, 1440, 1508, 1578, 1631, 1688, 1754, 1816, 1877, 1945, 2003, 2060, 2125, 2183, 2253, 2313, 2379, 2436, 2513, 2570, 2626, 2702, 2757, 2809, 2885, 2945, 3006, 3067, 3128, 3180, 3237, 3290, 3347, 3397, 3462, 3520, 3585, 3651, 3706, 3755]}, {"subject_area"=>"/Medicine and health sciences/Anatomy and physiology", "average_usage"=>[270, 536, 692, 812, 932, 1037, 1128, 1210, 1293, 1367, 1439, 1514, 1580, 1646, 1711, 1766, 1834, 1902, 1969, 2036, 2106, 2165, 2221, 2285, 2337, 2403, 2459, 2517, 2586, 2644, 2716, 2782, 2842, 2895, 2958, 3017]}]}
Loading … Spinner
There are currently no alerts