Mechanical Strain Stabilizes Reconstituted Collagen Fibrils against Enzymatic Degradation by Mammalian Collagenase Matrix Metalloproteinase 8 (MMP-8)
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Mechanical strain stabilizes reconstituted collagen fibrils against enzymatic degradation by mammalian collagenase matrix metalloproteinase 8 (MMP-8)", "type"=>"journal", "authors"=>[{"first_name"=>"Brendan P.", "last_name"=>"Flynn", "scopus_author_id"=>"34968956500"}, {"first_name"=>"Amit P.", "last_name"=>"Bhole", "scopus_author_id"=>"34969092600"}, {"first_name"=>"Nima", "last_name"=>"Saeidi", "scopus_author_id"=>"34267833700"}, {"first_name"=>"Melody", "last_name"=>"Liles", "scopus_author_id"=>"56234256700"}, {"first_name"=>"Charles A.", "last_name"=>"Dimarzio", "scopus_author_id"=>"7003299874"}, {"first_name"=>"Jeffrey W.", "last_name"=>"Ruberti", "scopus_author_id"=>"6603541449"}], "year"=>2010, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"20808784", "doi"=>"10.1371/journal.pone.0012337", "sgr"=>"77957873845", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "scopus"=>"2-s2.0-77957873845", "issn"=>"19326203", "pui"=>"359754364"}, "id"=>"3a4ed324-35da-3f62-b32b-2b68ca6ef4ec", "abstract"=>"BACKGROUND: Collagen, a triple-helical, self-organizing protein, is the predominant structural protein in mammals. It is found in bone, ligament, tendon, cartilage, intervertebral disc, skin, blood vessel, and cornea. We have recently postulated that fibrillar collagens (and their complementary enzymes) comprise the basis of a smart structural system which appears to support the retention of molecules in fibrils which are under tensile mechanical strain. The theory suggests that the mechanisms which drive the preferential accumulation of collagen in loaded tissue operate at the molecular level and are not solely cell-driven. The concept reduces control of matrix morphology to an interaction between molecules and the most relevant, physical, and persistent signal: mechanical strain.\\n\\nMETHODOLOGY/PRINCIPAL FINDINGS: The investigation was carried out in an environmentally-controlled microbioreactor in which reconstituted type I collagen micronetworks were gently strained between micropipettes. The strained micronetworks were exposed to active matrix metalloproteinase 8 (MMP-8) and relative degradation rates for loaded and unloaded fibrils were tracked simultaneously using label-free differential interference contrast (DIC) imaging. It was found that applied tensile mechanical strain significantly increased degradation time of loaded fibrils compared to unloaded, paired controls. In many cases, strained fibrils were detectable long after unstrained fibrils were degraded.\\n\\nCONCLUSIONS/SIGNIFICANCE: In this investigation we demonstrate for the first time that applied mechanical strain preferentially preserves collagen fibrils in the presence of a physiologically-important mammalian enzyme: MMP-8. These results have the potential to contribute to our understanding of many collagen matrix phenomena including development, adaptation, remodeling and disease. Additionally, tissue engineering could benefit from the ability to sculpt desired structures from physiologically compatible and mutable collagen.", "link"=>"http://www.mendeley.com/research/mechanical-strain-stabilizes-reconstituted-collagen-fibrils-against-enzymatic-degradation-mammalian", "reader_count"=>127, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>9, "Librarian"=>1, "Student > Doctoral Student"=>6, "Researcher"=>20, "Student > Ph. D. Student"=>40, "Student > Postgraduate"=>4, "Other"=>6, "Student > Master"=>18, "Student > Bachelor"=>9, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>11}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>9, "Librarian"=>1, "Student > Doctoral Student"=>6, "Researcher"=>20, "Student > Ph. D. Student"=>40, "Student > Postgraduate"=>4, "Other"=>6, "Student > Master"=>18, "Student > Bachelor"=>9, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>11}, "reader_count_by_subject_area"=>{"Unspecified"=>7, "Agricultural and Biological Sciences"=>30, "Business, Management and Accounting"=>1, "Chemical Engineering"=>1, "Chemistry"=>4, "Engineering"=>49, "Biochemistry, Genetics and Molecular Biology"=>7, "Nursing and Health Professions"=>1, "Materials Science"=>4, "Mathematics"=>2, "Medicine and Dentistry"=>17, "Physics and Astronomy"=>3, "Social Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Materials Science"=>{"Materials Science"=>4}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>17}, "Social Sciences"=>{"Social Sciences"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>3}, "Mathematics"=>{"Mathematics"=>2}, "Unspecified"=>{"Unspecified"=>7}, "Chemical Engineering"=>{"Chemical Engineering"=>1}, "Engineering"=>{"Engineering"=>49}, "Chemistry"=>{"Chemistry"=>4}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>30}, "Business, Management and Accounting"=>{"Business, Management and Accounting"=>1}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>7}}, "reader_count_by_country"=>{"Canada"=>1, "Argentina"=>1, "Netherlands"=>1, "United States"=>3, "China"=>1, "Denmark"=>1, "Poland"=>1, "United Kingdom"=>2, "Germany"=>1, "Spain"=>1}, "group_count"=>14}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/77957873845"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/77957873845?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77957873845&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=77957873845&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/77957873845", "dc:identifier"=>"SCOPUS_ID:77957873845", "eid"=>"2-s2.0-77957873845", "dc:title"=>"Mechanical strain stabilizes reconstituted collagen fibrils against enzymatic degradation by mammalian collagenase matrix metalloproteinase 8 (MMP-8)", "dc:creator"=>"Flynn B.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"5", "prism:issueIdentifier"=>"8", "prism:pageRange"=>nil, "prism:coverDate"=>"2010-10-19", "prism:coverDisplayDate"=>"2010", "prism:doi"=>"10.1371/journal.pone.0012337", "citedby-count"=>"96", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Northeastern University", "affiliation-city"=>"Boston", "affiliation-country"=>"United States"}], "pubmed-id"=>"20808784", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e12337", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0012337", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"8", "year"=>"2010", "pdf_views"=>"23", "xml_views"=>"3", "html_views"=>"155"}
  • {"month"=>"9", "year"=>"2010", "pdf_views"=>"71", "xml_views"=>"3", "html_views"=>"202"}
  • {"month"=>"10", "year"=>"2010", "pdf_views"=>"24", "xml_views"=>"2", "html_views"=>"105"}
  • {"month"=>"11", "year"=>"2010", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"86"}
  • {"month"=>"12", "year"=>"2010", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"1", "year"=>"2011", "pdf_views"=>"9", "xml_views"=>"3", "html_views"=>"46"}
  • {"month"=>"2", "year"=>"2011", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"92"}
  • {"month"=>"3", "year"=>"2011", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"4", "year"=>"2011", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"5", "year"=>"2011", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"43"}
  • {"month"=>"6", "year"=>"2011", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"41"}
  • {"month"=>"7", "year"=>"2011", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"8", "year"=>"2011", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"9", "year"=>"2011", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"10", "year"=>"2011", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"11", "year"=>"2011", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"72"}
  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"49"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"62"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"53"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"3", "xml_views"=>"5", "html_views"=>"29"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"72"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"99"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"68"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"3", "html_views"=>"77"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"90"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"70"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"83"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"38"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"44"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"52"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"42"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"36"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"52"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"69"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"78"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"78"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"69"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"51"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"47"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"3", "html_views"=>"42"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"41"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"36"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"35"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"19"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"3", "html_views"=>"22"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"14"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"30", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"23", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"17"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/834498"], "description"=>"<p>Each experiment began with collagen polymerized in a bioreactor, both between functionalized pipettes and in the periphery. For edge intensity integration, ROIs were selected, using the same general pattern each time, but choosing ideal locations in each section to monitor large perpendicular or parallel fibrils. Note the large perpendicular fibrils in ROI #4. These fibrils make an excellent visual control, for while they provide little weighted edge intensity, they are easy to track visually and are ideal control fibrils – they should experience little or no strain. Bar  = 20 µm.</p>", "links"=>[], "tags"=>["setup", "roi"], "article_id"=>504864, "categories"=>["Biophysics", "Biotechnology", "Developmental Biology", "Biochemistry", "Chemistry", "Medicine", "Cell Biology"], "users"=>["Brendan P. Flynn", "Amit P. Bhole", "Nima Saeidi", "Melody Liles", "Charles A. DiMarzio", "Jeffrey W. Ruberti"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0012337.g005", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Experimental_setup_and_ROI_selection_/504864", "title"=>"Experimental setup and ROI selection.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-08-23 01:21:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/834073"], "description"=>"<p>(A–C, G–I) DIC image series of degradation, with intensity adjusted. (D–F, J–L) Edge detected intensity image series of degradation. In strained ROI#8, the direction of strain, and the orientation of the predominant strained fibrils, is northwest-southeast (compare with <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0012337#pone-0012337-g005\" target=\"_blank\">Fig. 5</a>). Black arrows denote strained fibrils, white arrows denote unstrained fibrils. Note how quickly the perpendicular, unstrained fibrils (seen as high contrast dots) fade compared to strained fibrils. Also note relatively unstrained fibrils in the strained ROI degrade much faster than strained fibrils. Bar  = 5 µm. (see SI movie for video of this sequence and accompanying text).</p>", "links"=>[], "tags"=>["detection", "loaded", "roi", "unloaded"], "article_id"=>504432, "categories"=>["Biophysics", "Biotechnology", "Developmental Biology", "Biochemistry", "Chemistry", "Medicine", "Cell Biology"], "users"=>["Brendan P. Flynn", "Amit P. Bhole", "Nima Saeidi", "Melody Liles", "Charles A. DiMarzio", "Jeffrey W. Ruberti"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0012337.g001", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_DIC_and_Edge_detection_results_for_loaded_ROI_8_A_F_and_unloaded_ROI_4_G_L_from_Fig_5_/504432", "title"=>"DIC and Edge detection results for loaded ROI #8 (A–F) and unloaded ROI #4 (G–L) from Fig. 5.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-08-23 01:13:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/834213"], "description"=>"<p>Experimental time series images show the micropipettes drifting 30–40 µm apart, without fracturing or significantly thinning attached collagen fibrils, indicating either additional collagen deposition or structural changes within the fibrils. Note, in the last frame, stretched fibrils remain visible in the microbioreactor while peripheral fibrils have degraded. Experiments with large pipette drift were excluded from analysis. Bar  = 10 µm.</p>", "links"=>[], "tags"=>["pipette", "stretching", "fibrils", "enzymatic"], "article_id"=>504578, "categories"=>["Biophysics", "Biotechnology", "Developmental Biology", "Biochemistry", "Chemistry", "Medicine", "Cell Biology"], "users"=>["Brendan P. Flynn", "Amit P. Bhole", "Nima Saeidi", "Melody Liles", "Charles A. DiMarzio", "Jeffrey W. Ruberti"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0012337.g002", "stats"=>{"downloads"=>2, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_DIC_image_showing_pipette_drift_stretching_fibrils_to_over_100_initial_length_during_enzymatic_degradation_/504578", "title"=>"DIC image showing pipette drift stretching fibrils to over 100% initial length during enzymatic degradation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-08-23 01:16:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/415444", "https://ndownloader.figshare.com/files/415473"], "description"=>"<div><h3>Background</h3><p>Collagen, a triple-helical, self-organizing protein, is the predominant structural protein in mammals. It is found in bone, ligament, tendon, cartilage, intervertebral disc, skin, blood vessel, and cornea. We have recently postulated that fibrillar collagens (and their complementary enzymes) comprise the basis of a smart structural system which appears to support the retention of molecules in fibrils which are under tensile mechanical strain. The theory suggests that the mechanisms which drive the preferential accumulation of collagen in loaded tissue operate at the molecular level and are not solely cell-driven. The concept reduces control of matrix morphology to an interaction between molecules and the most relevant, physical, and persistent signal: mechanical strain.</p><h3>Methodology/Principal Findings</h3><p>The investigation was carried out in an environmentally-controlled microbioreactor in which reconstituted type I collagen micronetworks were gently strained between micropipettes. The strained micronetworks were exposed to active matrix metalloproteinase 8 (MMP-8) and relative degradation rates for loaded and unloaded fibrils were tracked simultaneously using label-free differential interference contrast (DIC) imaging. It was found that applied tensile mechanical strain significantly increased degradation time of loaded fibrils compared to unloaded, paired controls. In many cases, strained fibrils were detectable long after unstrained fibrils were degraded.</p><h3>Conclusions/Significance</h3><p>In this investigation we demonstrate for the first time that applied mechanical strain preferentially preserves collagen fibrils in the presence of a physiologically-important mammalian enzyme: MMP-8. These results have the potential to contribute to our understanding of many collagen matrix phenomena including development, adaptation, remodeling and disease. Additionally, tissue engineering could benefit from the ability to sculpt desired structures from physiologically compatible and mutable collagen.</p></div>", "links"=>[], "tags"=>["stabilizes", "reconstituted", "collagen", "fibrils", "enzymatic", "degradation", "mammalian", "collagenase", "matrix", "metalloproteinase"], "article_id"=>142026, "categories"=>["Biophysics", "Biotechnology", "Developmental Biology", "Biochemistry", "Chemistry", "Medicine", "Cell Biology"], "users"=>["Brendan P. Flynn", "Amit P. Bhole", "Nima Saeidi", "Melody Liles", "Charles A. DiMarzio", "Jeffrey W. Ruberti"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0012337.s001", "https://dx.doi.org/10.1371/journal.pone.0012337.s002"], "stats"=>{"downloads"=>23, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Mechanical_Strain_Stabilizes_Reconstituted_Collagen_Fibrils_against_Enzymatic_Degradation_by_Mammalian_Collagenase_Matrix_Metalloproteinase_8_MMP_8_/142026", "title"=>"Mechanical Strain Stabilizes Reconstituted Collagen Fibrils against Enzymatic Degradation by Mammalian Collagenase Matrix Metalloproteinase 8 (MMP-8)", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2010-08-23 00:33:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/834409"], "description"=>"<p>Note the differences between total and directional edge intensity methods. While both methods show significance (p<0.05) over a large range of R<sub>I</sub>, the total edge intensity is statistically different over almost the entire regime [.19, .81], while directional edge intensity is statistically different over the range from [.12, .53].</p>", "links"=>[], "tags"=>["degradation", "directional"], "article_id"=>504778, "categories"=>["Biophysics", "Biotechnology", "Developmental Biology", "Biochemistry", "Chemistry", "Medicine", "Cell Biology"], "users"=>["Brendan P. Flynn", "Amit P. Bhole", "Nima Saeidi", "Melody Liles", "Charles A. DiMarzio", "Jeffrey W. Ruberti"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0012337.g004", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_P_value_for_degradation_end_time_vs_R_I_for_total_edge_intensity_o_and_directional_edge_intensity_/504778", "title"=>"P-value for degradation end time vs. R<sub>I</sub> for total edge intensity (o) and directional edge intensity (+).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-08-23 01:19:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/834341"], "description"=>"<p>Typically, the noise threshold of the edge intensity algorithm is somewhere between R<sub>I</sub> [0.1, 0.2]. Note the large differences in degradation time between loaded and unloaded ROIs when R<sub>I</sub>  = 0.1 or R<sub>I</sub>  = 0.2 are used as end points (110 or 120 min. respectively).</p>", "links"=>[], "tags"=>["loaded", "unloaded", "rois", "shown"], "article_id"=>504703, "categories"=>["Biophysics", "Biotechnology", "Developmental Biology", "Biochemistry", "Chemistry", "Medicine", "Cell Biology"], "users"=>["Brendan P. Flynn", "Amit P. Bhole", "Nima Saeidi", "Melody Liles", "Charles A. DiMarzio", "Jeffrey W. Ruberti"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0012337.g003", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Normalized_edge_intensity_vs_time_minutes_for_loaded_solid_line_and_unloaded_dashed_line_ROIs_shown_in_fig_5_/504703", "title"=>"Normalized edge intensity vs. time (minutes) for loaded (solid line) and unloaded (dashed line) ROIs shown in fig. 5.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-08-23 01:18:23"}

PMC Usage Stats | Further Information

  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"4", "full-text"=>"43", "unique-ip"=>"42", "pdf"=>"29", "year"=>"2010", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"25", "year"=>"2010", "pdf"=>"15", "unique-ip"=>"23", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"49", "unique-ip"=>"41", "pdf"=>"37", "year"=>"2010", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"22", "year"=>"2010", "pdf"=>"14", "unique-ip"=>"23", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"3"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"24", "unique-ip"=>"19", "pdf"=>"16", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"4", "full-text"=>"24", "year"=>"2011", "pdf"=>"21", "unique-ip"=>"28", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"24", "unique-ip"=>"24", "pdf"=>"20", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"22", "year"=>"2011", "pdf"=>"7", "unique-ip"=>"19", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"17", "unique-ip"=>"19", "pdf"=>"9", "year"=>"2011", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"22", "year"=>"2011", "pdf"=>"18", "unique-ip"=>"19", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"10", "unique-ip"=>"10", "pdf"=>"7", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"13", "year"=>"2011", "pdf"=>"5", "unique-ip"=>"12", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"9", "unique-ip"=>"9", "pdf"=>"6", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"18", "year"=>"2011", "pdf"=>"8", "unique-ip"=>"18", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"16", "unique-ip"=>"15", "pdf"=>"8", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"5", "year"=>"2011", "pdf"=>"2", "unique-ip"=>"6", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"12", "unique-ip"=>"12", "pdf"=>"6", "year"=>"2012", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"7", "year"=>"2012", "pdf"=>"7", "unique-ip"=>"10", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"1", "abstract"=>"0", "full-text"=>"14", "unique-ip"=>"12", "pdf"=>"8", "year"=>"2012", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"11", "year"=>"2012", "pdf"=>"4", "unique-ip"=>"10", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"7", "unique-ip"=>"9", "pdf"=>"7", "year"=>"2012", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"4", "year"=>"2012", "pdf"=>"4", "unique-ip"=>"5", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"15", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"15", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"3", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"3", "cited-by"=>"4", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"16", "full-text"=>"17", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"13", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"18", "full-text"=>"20", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"15", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"22", "full-text"=>"23", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"18", "full-text"=>"18", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"25", "full-text"=>"25", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"3", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"13", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2010-01-01T00:00:00Z", "end_date"=>"2010-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Computer and information sciences", "average_usage"=>[375, 666, 837, 949, 1062, 1171, 1233, 1318, 1399, 1470, 1550, 1608, 1701, 1771, 1835, 1908, 1973, 2046, 2091, 2160, 2220, 2285, 2359, 2455, 2537, 2610, 2687, 2778, 2836, 2908, 2974, 3021, 3082, 3169, 3224, 3300, 3382, 3469, 3550, 3640, 3728, 3765, 3814, 3858, 3915, 3952, 3991, 4067, 4107]}, {"subject_area"=>"/Computer and information sciences/Information technology", "average_usage"=>[348, 644, 769, 882, 976, 1040, 1128, 1209, 1272, 1322, 1411, 1506, 1576, 1641, 1700, 1753, 1794, 1857, 1922, 1991, 2058, 2147, 2203, 2266, 2337, 2418, 2509, 2575, 2639, 2714, 2782, 2838, 2900, 2958, 3009, 3137, 3215, 3339, 3435, 3548, 3641, 3737, 3806, 3868, 3930, 3991, 4050, 4101, 4151]}, {"subject_area"=>"/Engineering and technology", "average_usage"=>[318, 573, 707, 808, 912, 1021, 1093, 1150, 1214, 1302, 1370, 1431, 1494, 1568, 1629, 1698, 1775, 1829, 1889, 1954, 2007, 2060, 2128, 2170, 2208, 2247, 2311, 2357, 2410, 2453, 2525, 2602, 2660, 2715, 2764, 2818, 2863, 2905, 2970, 3018, 3072, 3127, 3179, 3241, 3298, 3341, 3384, 3433, 3474]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[277, 558, 715, 845, 963, 1070, 1166, 1258, 1336, 1410, 1485, 1561, 1638, 1707, 1767, 1834, 1896, 1960, 2030, 2096, 2165, 2229, 2300, 2368, 2425, 2489, 2553, 2617, 2679, 2743, 2814, 2873, 2941, 2995, 3050, 3111, 3169, 3235, 3300, 3363, 3428, 3487, 3548, 3605, 3670, 3726, 3785, 3844]}, {"subject_area"=>"/Medicine and health sciences/Anatomy", "average_usage"=>[268, 527, 681, 804, 923, 1031, 1126, 1210, 1284, 1361, 1440, 1508, 1578, 1631, 1688, 1754, 1816, 1877, 1945, 2003, 2060, 2125, 2183, 2253, 2313, 2379, 2436, 2513, 2570, 2626, 2702, 2757, 2809, 2885, 2945, 3006, 3067, 3128, 3180, 3237, 3290, 3347, 3397, 3462, 3520, 3585, 3651, 3706, 3755]}]}
Loading … Spinner
There are currently no alerts