Human Stem Cell-Derived Neurons: A System to Study Human Tau Function and Dysfunction
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Human stem cell-derived neurons: A system to study human tau function and dysfunction", "type"=>"journal", "authors"=>[{"first_name"=>"Mariangela", "last_name"=>"Iovino", "scopus_author_id"=>"57198234129"}, {"first_name"=>"Rickie", "last_name"=>"Patani", "scopus_author_id"=>"16302224400"}, {"first_name"=>"Colin", "last_name"=>"Watts", "scopus_author_id"=>"57189639274"}, {"first_name"=>"Siddharthan", "last_name"=>"Chandran", "scopus_author_id"=>"7004332041"}, {"first_name"=>"Maria Grazia", "last_name"=>"Spillantini", "scopus_author_id"=>"7006722317"}], "year"=>2010, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"78649732405", "pmid"=>"21085657", "isbn"=>"1932-6203", "pui"=>"360084524", "issn"=>"19326203", "scopus"=>"2-s2.0-78649732405", "doi"=>"10.1371/journal.pone.0013947"}, "id"=>"52fc3f1c-6f7e-3014-bcc2-692cab22aa29", "abstract"=>"BACKGROUND: Intracellular filamentous deposits containing microtubule-associated protein tau constitute a defining characteristic of many neurodegenerative disorders. Current experimental models to study tau pathology in vitro do not usually recapitulate the tau expression pattern characteristic of adult human brain. In this study, we have investigated whether human embryonic stem cell-derived neurons could be a good model to study human tau distribution, function and dysfunction.\\n\\nMETHODOLOGY/PRINCIPAL FINDINGS: Using RT-PCR, immunohistochemistry, western blotting and cell transfections we have investigated whether all 6 adult human brain tau isoforms are expressed in neurons derived from human embryonic and fetal stem cells and whether 4 repeat tau over-expression alone, or with the F3 tau repeat fragment, (amino acid 258-380 of the 2N4R tau isoform with the ΔK280 mutation) affects tau distribution. We found that the shortest 3 repeat tau isoform, similarly to human brain, is the first to be expressed during neuronal differentiation while the other 5 tau isoforms are expressed later. Over expression of tau with 4 repeats affects tau cellular distribution and the short tau F3 fragment appears to increase tau phosphorylation but this effect does not appear to be toxic for the cell.\\n\\nCONCLUSIONS: Our results indicate that human embryonic stem cell-derived neurons express all 6 tau isoforms and are a good model in which to study tau physiology and pathology.", "link"=>"http://www.mendeley.com/research/human-stem-cellderived-neurons-system-study-human-tau-function-dysfunction-3", "reader_count"=>49, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>16, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>16, "Student > Postgraduate"=>5, "Other"=>3, "Student > Master"=>3, "Student > Bachelor"=>4}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>16, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>16, "Student > Postgraduate"=>5, "Other"=>3, "Student > Master"=>3, "Student > Bachelor"=>4}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>6, "Agricultural and Biological Sciences"=>24, "Medicine and Dentistry"=>6, "Neuroscience"=>8, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Business, Management and Accounting"=>1, "Chemistry"=>2}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>6}, "Neuroscience"=>{"Neuroscience"=>8}, "Chemistry"=>{"Chemistry"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>24}, "Business, Management and Accounting"=>{"Business, Management and Accounting"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"United States"=>1, "United Kingdom"=>2}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/78649732405"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/78649732405?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=78649732405&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=78649732405&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/78649732405", "dc:identifier"=>"SCOPUS_ID:78649732405", "eid"=>"2-s2.0-78649732405", "dc:title"=>"Human stem cell-derived neurons: A system to study human tau function and dysfunction", "dc:creator"=>"Iovino M.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"5", "prism:issueIdentifier"=>"11", "prism:pageRange"=>nil, "prism:coverDate"=>"2010-12-09", "prism:coverDisplayDate"=>"2010", "prism:doi"=>"10.1371/journal.pone.0013947", "citedby-count"=>"20", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"John van Geest Centre for Brain Repair", "affiliation-city"=>"Cambridge", "affiliation-country"=>"United Kingdom"}], "pubmed-id"=>"21085657", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e13947", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0013947", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"11", "year"=>"2010", "pdf_views"=>"95", "xml_views"=>"3", "html_views"=>"319"}
  • {"month"=>"12", "year"=>"2010", "pdf_views"=>"43", "xml_views"=>"0", "html_views"=>"81"}
  • {"month"=>"1", "year"=>"2011", "pdf_views"=>"27", "xml_views"=>"2", "html_views"=>"51"}
  • {"month"=>"2", "year"=>"2011", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"3", "year"=>"2011", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"4", "year"=>"2011", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"5", "year"=>"2011", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"29"}
  • {"month"=>"6", "year"=>"2011", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"7", "year"=>"2011", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"8", "year"=>"2011", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"9", "year"=>"2011", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"10", "year"=>"2011", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"11", "year"=>"2011", "pdf_views"=>"19", "xml_views"=>"5", "html_views"=>"42"}
  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"29"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"34"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"17", "xml_views"=>"5", "html_views"=>"25"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"37"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"50", "xml_views"=>"1", "html_views"=>"41"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"36"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"3", "html_views"=>"39"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"74"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"50"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"91"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"152"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"28"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"51"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"62"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"95"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"86"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"35"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"53"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"66"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"53"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"75"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"71"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"54", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"39"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"3", "html_views"=>"32"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"3", "html_views"=>"27"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"24"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"3", "html_views"=>"5"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"18"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/819008"], "description"=>"<p>hESC-derived neurons transfected with the shortest 4R tau (0N4R) at 21 DIV <b>(A)</b> and 35 DIV <b>(B)</b>. <b>(A)</b>: Immunocytochemistry for 4R tau (green), β-IIItubulin (red) and DAPI (blue) in control cells (a–d) and in transfected cells (e–h). Immunocytochemistry shows that in transfected cells 4R tau is present also in the axons of neurons and not only in the cell bodies as in control cells. <b>(B)</b>: There is no difference in the distribution of 4R tau in control cells (i–l) and in 4R tau transfected cells (m–p) when transfection is performed at 35 DIV although, in axons of transfected cells the intensity of fluorescence is increased. Immunocytochemistry with anti-4R tau antibody (E10) is performed at 72 hrs after transfection.</p>", "links"=>[], "tags"=>["tau", "over-expression"], "article_id"=>489379, "categories"=>["Neuroscience", "Cell Biology"], "users"=>["Mariangela Iovino", "Rickie Patani", "Colin Watts", "Siddharthan Chandran", "Maria Grazia Spillantini"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0013947.g006", "stats"=>{"downloads"=>1, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Four_repeat_tau_over_expression_A_8211_B_/489379", "title"=>"Four repeat tau over-expression (A–B).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 22:24:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/818804"], "description"=>"<p><b>(A)</b> (a–d): immunocytochemistry for 3R tau (green), β-IIItubulin (red) and DAPI (blue) and <b>(B)</b> (e–h) for 4R tau (green), β-IIItubulin (red) and DAPI (blue) in primary culture of biopsy derived human adult neurons. Both isoforms co-localize with β-IIItubulin and are similarly expressed in neuronal cell bodies and axons.</p>", "links"=>[], "tags"=>["cell biology/neuronal and glial cell biology", "neuroscience/neurobiology of disease and regeneration", "neuroscience/neuronal and glial cell biology", "neurological disorders/alzheimer disease"], "article_id"=>489172, "categories"=>["Neuroscience", "Cell Biology"], "users"=>["Mariangela Iovino", "Rickie Patani", "Colin Watts", "Siddharthan Chandran", "Maria Grazia Spillantini"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0013947.g004", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Tau_expression_in_adult_brain_biopsy_/489172", "title"=>"Tau expression in adult brain biopsy:", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 22:23:42"}
  • {"files"=>["https://ndownloader.figshare.com/files/818535"], "description"=>"<p><b>(A)</b> Immunostaining with anti-4R tau antibodies E10 (a–d) and RD4 (e–h) shows that 4R tau isoforms are present in neuronal cell bodies and axons. Both antibodies are 4R tau isoform-specific. <b>(B)</b> Tau isoform expression in hESC-derived neurons at 56 DIV. The immunoblot (human tau antibody, Dako) shows the tau isoforms before and after alkaline phosphatase (AP) treatment compared to human recombinant tau. Bands corresponding to the 6 tau isoforms can be seen and the shortest 3R tau isoform is the most abundant. De-phosphorylated tau extracted from adult human brain is also shown. The data are representative of at least three independent experiments. Similar results were obtained in both H9 and HuES cell lines.</p>", "links"=>[], "tags"=>["hesc-derived", "neurons", "72", "days"], "article_id"=>488901, "categories"=>["Neuroscience", "Cell Biology"], "users"=>["Mariangela Iovino", "Rickie Patani", "Colin Watts", "Siddharthan Chandran", "Maria Grazia Spillantini"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0013947.g002", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Tau_expression_in_hESC_derived_neurons_after_72_days_in_culture_/488901", "title"=>"Tau expression in hESC-derived neurons after 72 days in culture.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 22:22:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/818678"], "description"=>"<p><b>(A)</b> RT-PCR for 3R and 4R tau in hFSCs after 1 DIV, 7 DIV, 14 DIV and 21 DIV. Four repeat tau mRNA appears after 7 DIV and increases during neuronal differentiation. At 21DIV the level of expression of 3R tau is reduced. <b>(B)</b> (a–h): immunocytochemistry for 3R tau (green), β-IIItubulin (red) and DAPI (blue) in hFSC-derived neurons at 7 DIV (a–d) and 21 DIV (e–h). At 7 DIV 3R tau is strongly expressed in both cell bodies and axons as seen also at 21 DIV. (i–p): immunocytochemistry for 4R tau (green), β-IIItubulin (red) and DAPI (blue) in neurons at 7 DIV and 21 DIV. At 7 DIV 4R tau is visibile in cell bodies while at 21 DIV is present in both cell bodies and axons. <b>(C)</b> Immunoblot of tau isoforms extracted from hFSC-derived neurones. At 28 DIV only the two shortest 3R and 4R isoforms are visible while at 35 DIV and 56 DIV all six tau isoforms are present. Anti-human tau antibody (Dako, 1∶1000) is used for immunoblotting.</p>", "links"=>[], "tags"=>["hfsc-derived"], "article_id"=>489049, "categories"=>["Neuroscience", "Cell Biology"], "users"=>["Mariangela Iovino", "Rickie Patani", "Colin Watts", "Siddharthan Chandran", "Maria Grazia Spillantini"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0013947.g003", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Tau_expression_in_hFSC_derived_neurons_/489049", "title"=>"Tau expression in hFSC-derived neurons.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 22:23:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/818357"], "description"=>"<p><b>(A)</b> RT-PCR for 3R and 4R tau isoforms in hESCs before differentiation (hES) and in adult human brain. hESCs do not express tau protein while in the adult human brain the ratio between 3R and 4R tau mRNA is about 1∶1. <b>(B)</b> RT-PCR for 3R and 4R tau in hESCs at 7 DIV, 14 DIV, 21 DIV, 35 DIV, 72 DIV and adult brain tissue shows that 4R tau increases during neuronal differentiation. <b>(C)</b> (a–l): immunocytochemistry for 3R tau (green), β-IIItubulin (red) and DAPI (blue) after 14 DIV (a–d), 21 DIV (e–h) and 35 DIV (i–l). 3R tau co-localizes with β-IIItubulin and is strongly expressed in neuronal cell bodies and axons. (m–x): Immunocytochemistry for 4R tau (green), β-IIItubulin (red) and DAPI (blue) at 14 DIV (m–p), 21 DIV (q–t) and 35 DIV (u–x). Four repeat tau is not expressed at 14 DIV, but it starts to appear in cell bodies at 21 DIV. The expression of 4R tau becomes stronger at 35 DIV when is present also in axons. RD3 and RD4 antibodies were used to detect 3R and 4R tau.</p>", "links"=>[], "tags"=>["hesc-derived"], "article_id"=>488727, "categories"=>["Neuroscience", "Cell Biology"], "users"=>["Mariangela Iovino", "Rickie Patani", "Colin Watts", "Siddharthan Chandran", "Maria Grazia Spillantini"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0013947.g001", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Tau_expression_in_hESC_derived_neurons_/488727", "title"=>"Tau expression in hESC-derived neurons.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 22:21:16"}
  • {"files"=>["https://ndownloader.figshare.com/files/819177"], "description"=>"<p><b>(A)</b> Immunostaining with AT8 antibody in hESC-derived neurons transfected at 35 DIV with the shorter 4R tau isoform (c–d) and empty vector (a–b). The over-expression of 4R tau isoform does not increase tau hyperphosphorylation. <b>(e–h)</b> Immunostaining with AT8 antibody in hESC-derived neurons co-transfected at 35 DIV with F3 fragment and 0N4R tau (g–h) and cells transfected only with F3 fragment (e–f). An increase in AT8 staining is present in cells transfected with only F3 (e,f) compared to controls (a,b). Neurons co-transfected with F3 and 0N4R show tau hyperphosphorylation and more dotted appearance in processes (g,h). <b>(B)</b> Immunostaining with pSer262 anti-tau antibody in hESC-derived neurons co-transfected with F3 fragment and 0N4R tau at 35 DIV: neurons co-transfected with F3 fragment and 0N4R (c–d) show an increase in pSer262 staining compared to control cells (a–b).</p>", "links"=>[], "tags"=>["induces", "tau", "hyperphosphorylation"], "article_id"=>489544, "categories"=>["Neuroscience", "Cell Biology"], "users"=>["Mariangela Iovino", "Rickie Patani", "Colin Watts", "Siddharthan Chandran", "Maria Grazia Spillantini"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0013947.g007", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_F3_fragment_induces_tau_hyperphosphorylation_and_redistribution_/489544", "title"=>"F3 fragment induces tau hyperphosphorylation and redistribution.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 22:25:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/818900"], "description"=>"<p><b>(A)</b> hESC-derived neurons: quantification (Image J software) of 3R and 4R tau RT-PCR bands shows that the ratio between 3R and 4R tau isoforms becomes similar to that in adult human brain <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0013947#pone.0013947-Lee1\" target=\"_blank\">[1]</a> during neuronal differentiation. <b>(B)</b> hFSC-derived neurons: quantification (Image J software) of 3R and 4R tau RT-PCR bands shows that the time for differentiation of neurons derived from hFSCs is shorter than that for hESCs, at 21 DIV the ratio between 3R and 4R tau isoforms is already similar to that in adult human brain <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0013947#pone.0013947-Lee1\" target=\"_blank\">[1]</a>.</p>", "links"=>[], "tags"=>["quantification", "tau", "isoform"], "article_id"=>489267, "categories"=>["Neuroscience", "Cell Biology"], "users"=>["Mariangela Iovino", "Rickie Patani", "Colin Watts", "Siddharthan Chandran", "Maria Grazia Spillantini"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0013947.g005", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_RT_PCR_quantification_of_tau_isoform_expression_/489267", "title"=>"RT-PCR quantification of tau isoform expression.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 22:24:15"}

PMC Usage Stats | Further Information

  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"142", "unique-ip"=>"127", "pdf"=>"92", "year"=>"2010", "figure"=>"21", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"6", "full-text"=>"107", "year"=>"2010", "pdf"=>"81", "unique-ip"=>"103", "figure"=>"24", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"50", "unique-ip"=>"59", "pdf"=>"38", "year"=>"2011", "figure"=>"16", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"46", "year"=>"2011", "pdf"=>"28", "unique-ip"=>"39", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"6", "full-text"=>"30", "unique-ip"=>"35", "pdf"=>"21", "year"=>"2011", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"6", "full-text"=>"28", "year"=>"2011", "pdf"=>"16", "unique-ip"=>"31", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"5", "full-text"=>"34", "unique-ip"=>"36", "pdf"=>"33", "year"=>"2011", "figure"=>"14", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"23", "year"=>"2011", "pdf"=>"13", "unique-ip"=>"26", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"15", "unique-ip"=>"16", "pdf"=>"11", "year"=>"2011", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"13", "year"=>"2011", "pdf"=>"3", "unique-ip"=>"15", "figure"=>"6", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"14", "unique-ip"=>"13", "pdf"=>"10", "year"=>"2011", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"25", "year"=>"2011", "pdf"=>"7", "unique-ip"=>"23", "figure"=>"12", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"27", "unique-ip"=>"25", "pdf"=>"18", "year"=>"2011", "figure"=>"9", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"16", "year"=>"2011", "pdf"=>"6", "unique-ip"=>"13", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"19", "unique-ip"=>"21", "pdf"=>"6", "year"=>"2012", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"37", "year"=>"2012", "pdf"=>"9", "unique-ip"=>"30", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"24", "unique-ip"=>"17", "pdf"=>"11", "year"=>"2012", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"9", "year"=>"2012", "pdf"=>"2", "unique-ip"=>"10", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"8", "unique-ip"=>"13", "pdf"=>"5", "year"=>"2012", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"18", "year"=>"2012", "pdf"=>"9", "unique-ip"=>"12", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"8", "full-text"=>"13", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"15", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"24", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"15", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"19", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"27", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"16", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"14", "full-text"=>"10", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"18", "full-text"=>"15", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"18", "full-text"=>"18", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"14", "full-text"=>"24", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"14", "full-text"=>"17", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"17", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"17", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"18", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"15", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"18", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"10", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2010-01-01T00:00:00Z", "end_date"=>"2010-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[286, 573, 735, 872, 989, 1096, 1185, 1277, 1365, 1449, 1520, 1597, 1668, 1729, 1799, 1852, 1917, 1984, 2054, 2108, 2169, 2234, 2303, 2374, 2436, 2501, 2560, 2620, 2683, 2742, 2830, 2909, 2981, 3032, 3089, 3148, 3210, 3266, 3323, 3379, 3448, 3512, 3576, 3635, 3693, 3747, 3827, 3876, 3938]}, {"subject_area"=>"/People and places", "average_usage"=>[300, 565, 713, 834, 949, 1067, 1154, 1249, 1325, 1400, 1475, 1554, 1619, 1696, 1757, 1821, 1905, 1968, 2034, 2088, 2147, 2218, 2289, 2365, 2434, 2491, 2560, 2618, 2682, 2777, 2842, 2910, 2977, 3035, 3107, 3178, 3250, 3313, 3383, 3445, 3512, 3569, 3631, 3683, 3748, 3818, 3882, 3942]}, {"subject_area"=>"/People and places/Population groupings", "average_usage"=>[304, 560, 714, 827, 946, 1046, 1150, 1244, 1322, 1408, 1484, 1558, 1621, 1693, 1757, 1815, 1874, 1948, 2005, 2085, 2139, 2219, 2279, 2346, 2401, 2469, 2533, 2588, 2680, 2736, 2797, 2901, 2956, 2990, 3068, 3166, 3223, 3286, 3345, 3419, 3469, 3533, 3583, 3648, 3694, 3765, 3818, 3884]}]}
Loading … Spinner
There are currently no alerts