Amyloid Precursor Protein Binding Protein-1 Modulates Cell Cycle Progression in Fetal Neural Stem Cells
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Mendeley | Further Information

{"title"=>"Amyloid Precursor Protein Binding Protein-1 Modulates Cell Cycle Progression in Fetal Neural Stem Cells", "type"=>"journal", "authors"=>[{"first_name"=>"Yuyoung", "last_name"=>"Joo"}, {"first_name"=>"Sungji", "last_name"=>"Ha"}, {"first_name"=>"Bo-Hyun", "last_name"=>"Hong"}, {"first_name"=>"Jeong a", "last_name"=>"Kim"}, {"first_name"=>"Keun-A", "last_name"=>"Chang"}, {"first_name"=>"Hyunjeong", "last_name"=>"Liew"}, {"first_name"=>"Seonghan", "last_name"=>"Kim"}, {"first_name"=>"Woong", "last_name"=>"Sun"}, {"first_name"=>"Joung-Hun", "last_name"=>"Kim"}, {"first_name"=>"Young Hae", "last_name"=>"Chong"}, {"first_name"=>"Yoo-Hun", "last_name"=>"Suh"}, {"first_name"=>"Hye-Sun", "last_name"=>"Kim"}], "year"=>2010, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"1932-6203", "doi"=>"10.1371/journal.pone.0014203", "isbn"=>"2008000202750", "pmid"=>"21151996"}, "id"=>"41e6e330-db7d-3b52-8efd-6ca96e358c3d", "abstract"=>"Amyloid precursor protein binding protein-1 (APP-BP1) binds to the carboxyl terminus of the amyloid precursor protein (APP) and serves as the bipartite activation enzyme for the ubiquitin-like protein, NEDD8. In the present study, we explored the physiological role of APP-BP1 in the cell cycle progression of fetal neural stem cells. Our results show that cell cycle progression of the cells is arrested at the G1 phase by depletion of APP-BP1, which results in a marked decrease in the proliferation of the cells. This action of APP-BP1 is antagonistically regulated by the interaction with APP. Consistent with the evidence that APP-BP1 function is critical for cell cycle progression, the amount of APP-BP1 varies depending upon cell cycle phase, with culminating expression at S-phase. Furthermore, our FRET experiment revealed that phosphorylation of APP at threonine 668, known to occur during the G2/M phase, is required for the interaction between APP and APP-BP1. We also found a moderate ubiquitous level of APP-BP1 mRNA in developing embryonic and early postnatal brains; however, APP-BP1 expression is reduced by P12, and only low levels of APP-BP1 were found in the adult brain. In the cerebral cortex of E16 rats, substantial expression of both APP-BP1 and APP mRNAs was observed in the ventricular zone. Collectively, these results indicate that APP-BP1 plays an important role in the cell cycle progression of fetal neural stem cells, through the interaction with APP, which is fostered by phosphorylation of threonine 668.", "link"=>"http://www.mendeley.com/research/amyloid-precursor-protein-binding-protein1-modulates-cell-cycle-progression-fetal-neural-stem-cells-1", "reader_count"=>14, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>4, "Student > Ph. D. Student"=>4, "Student > Master"=>1, "Student > Bachelor"=>1, "Professor"=>2, "Student > Postgraduate"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>4, "Student > Ph. D. Student"=>4, "Student > Master"=>1, "Student > Bachelor"=>1, "Professor"=>2, "Student > Postgraduate"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>12}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>12}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}}, "reader_count_by_country"=>{"Australia"=>1}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/815185"], "description"=>"<p>HEK 293 cells on coverlips in 6-well plates were transiently co-transfected with pZsYellow-APP-BP1 and pAMCyan1-APP. Figures in the left panel show representative results from the seven independent experiments. The right upper panel shows a representative graph indicating the time-lapse fluorescence changes in the region of interest (ROI). Green line: AMCyan1 fluorescence; gray: ZsYellow throughout the progression of successive bleaching marked as vertical green bars. In the right lower panel, relative fluorescence intensity is denoted after standardization of intensities before and after photobleaching of ZsYellow at 514 nm. *<i>p</i><0.05 versus fluorescence intensity before photobleaching of ZsYellow at 514 nm (by Student's t-test). Scale bars = 50 µm.</p>", "links"=>[], "tags"=>["app-bp1", "app", "was", "examined"], "article_id"=>485538, "categories"=>["Neuroscience"], "users"=>["Yuyoung Joo", "Sungji Ha", "Bo-Hyun Hong", "Jeong a Kim", "Keun-A Chang", "Hyunjeong Liew", "Seonghan Kim", "Woong Sun", "Joung-Hun Kim", "Young Hae Chong", "Yoo-Hun Suh", "Hye-Sun Kim"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0014203.g009", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Molecular_interaction_between_APP_BP1_and_APP_was_examined_by_FRET_/485538", "title"=>"Molecular interaction between APP-BP1 and APP was examined by FRET.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-12-02 01:32:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/814702"], "description"=>"<p>Fetal neural stem cells were synchronized to G1 phase by treatment with 2 mM thymidine for 16 h, and then released from cell cycle arrest by replacing the thymidine-containing media. Cells were harvested at 0, 2, 4, 6, 8, 10 and 12 h, and APP-BP1 protein levels were examined by Western blotting. The blot is representative of three independent experiments. The protein level of GAPDH was examined as a loading control.</p>", "links"=>[], "tags"=>["varied"], "article_id"=>485059, "categories"=>["Neuroscience"], "users"=>["Yuyoung Joo", "Sungji Ha", "Bo-Hyun Hong", "Jeong a Kim", "Keun-A Chang", "Hyunjeong Liew", "Seonghan Kim", "Woong Sun", "Joung-Hun Kim", "Young Hae Chong", "Yoo-Hun Suh", "Hye-Sun Kim"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0014203.g005", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_APP_BP1_expression_varied_according_to_cell_cycle_phase_/485059", "title"=>"APP-BP1 expression varied according to cell cycle phase.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-12-02 01:24:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/814490"], "description"=>"<p>Fetal neural stem cells were subcultured in 6-well plates, in proliferating media, and then treated the cells with 10 nM siRNAs. After 24 h, the cells were treated with BrdU (1 µM) for 24 h. We then dissociated the neurospheres, and transferred the cells to new 6-well plates with 12 mm glass coverslips pre-coated with laminin (20 µg/ml). The cells were cultured in the differentiation media without growth factors for 30 min and assessed with immunocytochemistry using an anti BrdU and Cy3-secondary antibodies. DAPI (1 µM) staining shows the location of the nucleus (blue). DIC represents differential image contrast. BrdU immunoreactive cells were calculated in five independent experiments (10 fields were evaluated per each experiment) with respect to the ratio of BrdU stained cells to total cells. Data are expressed as mean ± SEM values. One-way ANOVA was used to determine statistical significance at <i>p</i><0.05 by post hoc analysis via Duncan's test. Scale bars = 50 µm.</p>", "links"=>[], "tags"=>["app", "knock-down", "increases", "proliferation", "fetal", "neural"], "article_id"=>484844, "categories"=>["Neuroscience"], "users"=>["Yuyoung Joo", "Sungji Ha", "Bo-Hyun Hong", "Jeong a Kim", "Keun-A Chang", "Hyunjeong Liew", "Seonghan Kim", "Woong Sun", "Joung-Hun Kim", "Young Hae Chong", "Yoo-Hun Suh", "Hye-Sun Kim"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0014203.g003", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_siRNA_mediated_APP_knock_down_significantly_increases_the_proliferation_of_fetal_neural_stem_cells_/484844", "title"=>"siRNA-mediated APP knock-down significantly increases the proliferation of fetal neural stem cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-12-02 01:20:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/814363"], "description"=>"<p>Fetal neural stem cells were subcultured in 6-well plates, in proliferating media, and then treated the cells with 10 nM siRNAs. After 24 h, the cells were treated with BrdU (1 µM) for 24 h. We then dissociated the neurospheres, and transferred the cells to new 6-well plates with 12 mm glass coverslips pre-coated with laminin (20 µg/ml). The cells were cultured in the differentiation media without growth factors for 30 min and assessed with immunocytochemistry using an anti BrdU and Cy3-secondary antibodies. DAPI (1 µM) staining shows the location of the nucleus (blue). DIC represents differential image contrast. BrdU immunoreactive cells were calculated in five independent experiments (10 fields were evaluated per each experiment) with respect to the ratio of BrdU stained cells to total cells. Data are expressed as mean ± SEM values. One-way ANOVA was used to determine statistical significance at <i>p</i><0.05 by post hoc analysis via Duncan's test. Scale bars = 50 µm.</p>", "links"=>[], "tags"=>["app-bp1", "knock-down", "decreases", "brdu-incorporated", "fetal", "neural"], "article_id"=>484716, "categories"=>["Neuroscience"], "users"=>["Yuyoung Joo", "Sungji Ha", "Bo-Hyun Hong", "Jeong a Kim", "Keun-A Chang", "Hyunjeong Liew", "Seonghan Kim", "Woong Sun", "Joung-Hun Kim", "Young Hae Chong", "Yoo-Hun Suh", "Hye-Sun Kim"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0014203.g002", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_siRNA_mediated_APP_BP1_knock_down_significantly_decreases_BrdU_incorporated_fetal_neural_stem_cells_/484716", "title"=>"siRNA-mediated APP-BP1 knock-down significantly decreases BrdU-incorporated fetal neural stem cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-12-02 01:18:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/814968"], "description"=>"<p>A list of DNA constructs used in FRET study (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0014203#pone-0014203-g008\" target=\"_blank\">Figures 8</a>–<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0014203#pone-0014203-g009\" target=\"_blank\"></a><a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0014203#pone-0014203-g010\" target=\"_blank\">10</a>) was shown.</p>", "links"=>[], "tags"=>["dna", "constructs", "fret"], "article_id"=>485325, "categories"=>["Neuroscience"], "users"=>["Yuyoung Joo", "Sungji Ha", "Bo-Hyun Hong", "Jeong a Kim", "Keun-A Chang", "Hyunjeong Liew", "Seonghan Kim", "Woong Sun", "Joung-Hun Kim", "Young Hae Chong", "Yoo-Hun Suh", "Hye-Sun Kim"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0014203.g007", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_A_list_of_DNA_constructs_used_in_FRET_experiments_/485325", "title"=>"A list of DNA constructs used in FRET experiments.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-12-02 01:28:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/814812"], "description"=>"<p>The distribution of APP (<b>A–E</b>) and APP-BP (<b>F–J</b>) mRNA was detected by ISH at E14 (<b>A, F</b>), E16 (<b>B, G</b>) embryos and P0 (<b>C, H</b>), P12 (<b>D, I</b>), and in adult (<b>E, J</b>) brains. Insets in <b>B</b> and <b>G</b> show a large magnification view of spinal cord. (<b>K–N</b>): Emersion-developed black silver grains of APP-BP (<b>K, M</b>) and APP (<b>L, N</b>) mRNA signal showed distribution in the E16 cerebral cortex (<b>K, L</b>) and P0 cerebellum (<b>M, N</b>). Insets in <b>K</b> and <b>L</b> show large magnification view of CP (upper insets) and VZ (lower insets), and the insets in <b>M</b> and <b>N</b> shows enlarged views of EGL. (<b>O, P</b>): Adult brain sections were hybridized with sense probes for APP-BP (<b>O</b>) or APP (<b>P</b>). Experiments were duplicated, and typical images are shown. Abbreviations: Ctx, cerebral cortex; Cbll, cerebellum; Sc, spinal cord; Ht, heart; Li, liver; Kd, kidney; Str, striatum; Th, thalamus; Hip, hippocampus; Ob, olfactory bulb; v, ventral spinal cord; d, dorsal spinal cord; CP, cortical plate; IZ, intermediate zone; VZ, ventricular zone; EGL, external germinal layer; PCL, Purkinje cell layer; IGL, inner granule cell layer; MZ/WM, marginal zone/white matter.</p>", "links"=>[], "tags"=>["changes", "app", "app-bp1"], "article_id"=>485168, "categories"=>["Neuroscience"], "users"=>["Yuyoung Joo", "Sungji Ha", "Bo-Hyun Hong", "Jeong a Kim", "Keun-A Chang", "Hyunjeong Liew", "Seonghan Kim", "Woong Sun", "Joung-Hun Kim", "Young Hae Chong", "Yoo-Hun Suh", "Hye-Sun Kim"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0014203.g006", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Developmental_changes_in_the_expression_of_APP_and_APP_BP1_were_examined_/485168", "title"=>"Developmental changes in the expression of APP and APP-BP1 were examined.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-12-02 01:26:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/814600"], "description"=>"<p>Fetal neural stem cells were subcultured in 6-well plates, in proliferating media, and then treated the cells with 10 nM siRNAs. After 24 h, the cells were treated with BrdU (1 µM) for 24 h. We then dissociated the neurospheres, and transferred the cells to new 6-well plates with 12 mm glass coverslips pre-coated with laminin (20 µg/ml). The cells were cultured in the differentiation media without growth factors for 30 min and assessed with immunocytochemistry using an anti BrdU and Cy3-secondary antibodies. DAPI (1 µM) staining shows the location of the nucleus (blue). DIC represents differential image contrast. BrdU immunoreactive cells were calculated in five independent experiments (10 fields were evaluated per each experiment) with respect to the ratio of BrdU stained cells to total cells. Data are expressed as mean ± SEM values. One-way ANOVA was used to determine statistical significance at <i>p</i><0.05 by post hoc analysis via Duncan's test. Scale bars = 50 µm.</p>", "links"=>[], "tags"=>["knock-down", "app-bp1", "app", "down-regulates", "proliferation", "fetal", "neural"], "article_id"=>484950, "categories"=>["Neuroscience"], "users"=>["Yuyoung Joo", "Sungji Ha", "Bo-Hyun Hong", "Jeong a Kim", "Keun-A Chang", "Hyunjeong Liew", "Seonghan Kim", "Woong Sun", "Joung-Hun Kim", "Young Hae Chong", "Yoo-Hun Suh", "Hye-Sun Kim"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0014203.g004", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_siRNA_mediated_knock_down_of_APP_BP1_and_APP_significantly_down_regulates_the_proliferation_of_fetal_neural_stem_cells_/484950", "title"=>"siRNA-mediated knock-down of APP-BP1 and APP significantly down-regulates the proliferation of fetal neural stem cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-12-02 01:22:30"}
  • {"files"=>["https://ndownloader.figshare.com/files/815333"], "description"=>"<p>HEK 293 cells on coverlips in 6-well plates were transiently co-transfected with pZsYellow-APP-BP1 and pAMCyan1-APP<sup>T668A</sup>. Figures in the left panel show representative results from the seven independent experiments. The right upper panel shows a representative graph indicating the time-lapse fluorescence changes in the region of interest (ROI). Green line: AMCyan1 fluorescence; gray: ZsYellow throughout the progression of successive bleaching marked as vertical green bars. In the right lower panel, relative fluorescence intensity is denoted after standardization of intensities before and after photobleaching of ZsYellow at 514 nm. *<i>p</i><0.05 versus fluorescence intensity before photobleaching of ZsYellow at 514 nm (by Student's t-test). Scale bars = 50 µm.</p>", "links"=>[], "tags"=>["app-bp1", "was", "examined"], "article_id"=>485688, "categories"=>["Neuroscience"], "users"=>["Yuyoung Joo", "Sungji Ha", "Bo-Hyun Hong", "Jeong a Kim", "Keun-A Chang", "Hyunjeong Liew", "Seonghan Kim", "Woong Sun", "Joung-Hun Kim", "Young Hae Chong", "Yoo-Hun Suh", "Hye-Sun Kim"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0014203.g010", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Molecular_interaction_between_APP_BP1_and_APP_T668A_was_examined_by_FRET_/485688", "title"=>"Molecular interaction between APP-BP1 and APP<sup>T668A</sup> was examined by FRET.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-12-02 01:34:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/814246"], "description"=>"<p>The DNA content of the siRNAs treated fetal neural stem cells (Passage 8) was evaluated by flow cytometry with a FACScan instrument. Each group represents the status of the cell cycle for 10,000 cells.</p>", "links"=>[], "tags"=>["knockdown", "arrests", "g1-phase", "fetal", "neural"], "article_id"=>484601, "categories"=>["Neuroscience"], "users"=>["Yuyoung Joo", "Sungji Ha", "Bo-Hyun Hong", "Jeong a Kim", "Keun-A Chang", "Hyunjeong Liew", "Seonghan Kim", "Woong Sun", "Joung-Hun Kim", "Young Hae Chong", "Yoo-Hun Suh", "Hye-Sun Kim"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0014203.g001", "stats"=>{"downloads"=>5, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_APP_BP1_knockdown_arrests_cell_cycle_at_G1_phase_in_fetal_neural_stem_cells_/484601", "title"=>"APP-BP1 knockdown arrests cell cycle at G1-phase in fetal neural stem cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-12-02 01:16:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/406346", "https://ndownloader.figshare.com/files/406364", "https://ndownloader.figshare.com/files/406385", "https://ndownloader.figshare.com/files/406397"], "description"=>"<div><p>Amyloid precursor protein binding protein-1 (APP-BP1) binds to the carboxyl terminus of the amyloid precursor protein (APP) and serves as the bipartite activation enzyme for the ubiquitin-like protein, NEDD8. In the present study, we explored the physiological role of APP-BP1 in the cell cycle progression of fetal neural stem cells. Our results show that cell cycle progression of the cells is arrested at the G1 phase by depletion of APP-BP1, which results in a marked decrease in the proliferation of the cells. This action of APP-BP1 is antagonistically regulated by the interaction with APP. Consistent with the evidence that APP-BP1 function is critical for cell cycle progression, the amount of APP-BP1 varies depending upon cell cycle phase, with culminating expression at S-phase. Furthermore, our FRET experiment revealed that phosphorylation of APP at threonine 668, known to occur during the G2/M phase, is required for the interaction between APP and APP-BP1. We also found a moderate ubiquitous level of APP-BP1 mRNA in developing embryonic and early postnatal brains; however, APP-BP1 expression is reduced by P12, and only low levels of APP-BP1 were found in the adult brain. In the cerebral cortex of E16 rats, substantial expression of both APP-BP1 and APP mRNAs was observed in the ventricular zone. Collectively, these results indicate that APP-BP1 plays an important role in the cell cycle progression of fetal neural stem cells, through the interaction with APP, which is fostered by phopshorylation of threonine 668.</p></div>", "links"=>[], "tags"=>["amyloid", "precursor", "binding", "protein-1", "modulates", "progression", "fetal", "neural", "cells"], "article_id"=>140287, "categories"=>["Neuroscience"], "users"=>["Yuyoung Joo", "Sungji Ha", "Bo-Hyun Hong", "Jeong a Kim", "Keun-A Chang", "Hyunjeong Liew", "Seonghan Kim", "Woong Sun", "Joung-Hun Kim", "Young Hae Chong", "Yoo-Hun Suh", "Hye-Sun Kim"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0014203.s001", "https://dx.doi.org/10.1371/journal.pone.0014203.s002", "https://dx.doi.org/10.1371/journal.pone.0014203.s003", "https://dx.doi.org/10.1371/journal.pone.0014203.s004"], "stats"=>{"downloads"=>3, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Amyloid_Precursor_Protein_Binding_Protein_1_Modulates_Cell_Cycle_Progression_in_Fetal_Neural_Stem_Cells/140287", "title"=>"Amyloid Precursor Protein Binding Protein-1 Modulates Cell Cycle Progression in Fetal Neural Stem Cells", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2010-12-02 00:04:47"}
  • {"files"=>["https://ndownloader.figshare.com/files/815055"], "description"=>"<p>HEK 293 cells on coverlips in 6-well plates were transiently co-transfected with pZsYellow vector and pAMCyan1 vector. Figures in the left panel show representative results from the seven independent experiments. The right upper panel shows a representative graph indicating the time-lapse fluorescence changes in the region of interest (ROI). Green line: AMCyan1 fluorescence; gray: ZsYellow throughout the progression of successive bleaching marked as vertical green bars. In the right lower panel, relative fluorescence intensity is denoted after standardization of intensities before and after photobleaching of ZsYellow at 514 nm. *<i>p</i><0.05 versus fluorescence intensity before photobleaching of ZsYellow at 514 nm (by Student's t-test). Scale bars = 50 µm.</p>", "links"=>[], "tags"=>["pzsyellow", "vector", "pamcyan1", "was", "examined"], "article_id"=>485407, "categories"=>["Neuroscience"], "users"=>["Yuyoung Joo", "Sungji Ha", "Bo-Hyun Hong", "Jeong a Kim", "Keun-A Chang", "Hyunjeong Liew", "Seonghan Kim", "Woong Sun", "Joung-Hun Kim", "Young Hae Chong", "Yoo-Hun Suh", "Hye-Sun Kim"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0014203.g008", "stats"=>{"downloads"=>2, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Molecular_interaction_between_pZsYellow_vector_and_pAMCyan1_vector_was_examined_by_FRET_/485407", "title"=>"Molecular interaction between pZsYellow vector and pAMCyan1 vector was examined by FRET.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-12-02 01:30:07"}

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  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
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  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
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  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
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  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
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  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
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Relative Metric

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