Role of p53 Serine 46 in p53 Target Gene Regulation
Publication Date
March 04, 2011
Journal
PLOS ONE
Authors
Leonie Smeenk, Simon J. Van Heeringen, Max Koeppel, Bianca Gilbert, et al
Volume
6
Issue
3
Pages
e17574
DOI
https://dx.plos.org/10.1371/journal.pone.0017574
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0017574
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/21394211
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3048874
Europe PMC
http://europepmc.org/abstract/MED/21394211
Web of Science
000288025500017
Scopus
79952349193
Mendeley
http://www.mendeley.com/research/role-p53-serine-46-p53-target-gene-regulation-4
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Role of p53 Serine 46 in p53 target gene regulation", "type"=>"journal", "authors"=>[{"first_name"=>"Leonie", "last_name"=>"Smeenk", "scopus_author_id"=>"24449479200"}, {"first_name"=>"Simon J.", "last_name"=>"van Heeringen", "scopus_author_id"=>"24448856300"}, {"first_name"=>"Max", "last_name"=>"Koeppel", "scopus_author_id"=>"23990626500"}, {"first_name"=>"Bianca", "last_name"=>"Gilbert", "scopus_author_id"=>"42361236600"}, {"first_name"=>"Eva", "last_name"=>"Janssen-Megens", "scopus_author_id"=>"26026973300"}, {"first_name"=>"Hendrik G.", "last_name"=>"Stunnenberg", "scopus_author_id"=>"7006024572"}, {"first_name"=>"Marion", "last_name"=>"Lohrum", "scopus_author_id"=>"57199570013"}], "year"=>2011, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"361390388", "issn"=>"19326203", "isbn"=>"1932-6203 (Electronic) 1932-6203 (Linking)", "doi"=>"10.1371/journal.pone.0017574", "scopus"=>"2-s2.0-79952349193", "pmid"=>"21394211", "sgr"=>"79952349193"}, "id"=>"0613c3f0-1a9c-322e-a5ab-4f5bbd4783ac", "abstract"=>"The tumor suppressor p53 plays a crucial role in cellular growth control inducing a plethora of different response pathways. The molecular mechanisms that discriminate between the distinct p53-responses have remained largely elusive. Here, we have analyzed the p53-regulated pathways induced by Actinomycin D and Etoposide treatment resulting in more growth arrested versus apoptotic cells respectively. We found that the genome-wide p53 DNA-binding patterns are almost identical upon both treatments notwithstanding transcriptional differences that we observed in global transcriptome analysis. To assess the role of post-translational modifications in target gene choice and activation we investigated the genome-wide level of phosphorylation of Serine 46 of p53 bound to DNA (p53-pS46) and of Serine 15 (p53-pS15). Interestingly, the extent of S46 phosphorylation of p53 bound to DNA is considerably higher in cells directed towards apoptosis while the degree of phosphorylation at S15 remains highly similar. Moreover, our data suggest that following different chemotherapeutical treatments, the amount of chromatin-associated p53 phosphorylated at S46 but not at pS15 is higher on certain apoptosis related target genes. Our data provide evidence that cell fate decisions are not made primarily on the level of general p53 DNA-binding and that post-translationally modified p53 can have distinct DNA-binding characteristics.", "link"=>"http://www.mendeley.com/research/role-p53-serine-46-p53-target-gene-regulation-4", "reader_count"=>82, "reader_count_by_academic_status"=>{"Unspecified"=>3, "Professor > Associate Professor"=>3, "Librarian"=>1, "Researcher"=>18, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>28, "Student > Master"=>10, "Other"=>3, "Student > Bachelor"=>7, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>2, "Professor"=>4}, "reader_count_by_user_role"=>{"Unspecified"=>3, "Professor > Associate Professor"=>3, "Librarian"=>1, "Researcher"=>18, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>28, "Student > Master"=>10, "Other"=>3, "Student > Bachelor"=>7, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>2, "Professor"=>4}, "reader_count_by_subject_area"=>{"Unspecified"=>6, "Biochemistry, Genetics and Molecular Biology"=>17, "Mathematics"=>1, "Agricultural and Biological Sciences"=>40, "Medicine and Dentistry"=>10, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Chemistry"=>2, "Computer Science"=>3, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>10}, "Chemistry"=>{"Chemistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>40}, "Computer Science"=>{"Computer Science"=>3}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>17}, "Mathematics"=>{"Mathematics"=>1}, "Unspecified"=>{"Unspecified"=>6}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"Haiti"=>1, "Ecuador"=>1, "Netherlands"=>1, "United States"=>3, "Japan"=>1, "United Kingdom"=>3, "Portugal"=>1, "India"=>1}, "group_count"=>4}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/79952349193"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/79952349193?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79952349193&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=79952349193&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/79952349193", "dc:identifier"=>"SCOPUS_ID:79952349193", "eid"=>"2-s2.0-79952349193", "dc:title"=>"Role of p53 Serine 46 in p53 target gene regulation", "dc:creator"=>"Smeenk L.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"6", "prism:issueIdentifier"=>"3", "prism:pageRange"=>nil, "prism:coverDate"=>"2011-03-11", "prism:coverDisplayDate"=>"2011", "prism:doi"=>"10.1371/journal.pone.0017574", "citedby-count"=>"97", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Nijmegen Centre for Molecular Life Sciences - NCMLS", "affiliation-city"=>"Nijmegen", "affiliation-country"=>"Netherlands"}], "pubmed-id"=>"21394211", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e17574", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0017574", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"3", "year"=>"2011", "pdf_views"=>"111", "xml_views"=>"6", "html_views"=>"342"}
  • {"month"=>"4", "year"=>"2011", "pdf_views"=>"44", "xml_views"=>"1", "html_views"=>"121"}
  • {"month"=>"5", "year"=>"2011", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"6", "year"=>"2011", "pdf_views"=>"26", "xml_views"=>"1", "html_views"=>"69"}
  • {"month"=>"7", "year"=>"2011", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"8", "year"=>"2011", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"9", "year"=>"2011", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"10", "year"=>"2011", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"11", "year"=>"2011", "pdf_views"=>"30", "xml_views"=>"4", "html_views"=>"77"}
  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"33", "xml_views"=>"0", "html_views"=>"87"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"28", "xml_views"=>"0", "html_views"=>"82"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"19", "xml_views"=>"1", "html_views"=>"76"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"93"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"72"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"21", "xml_views"=>"5", "html_views"=>"79"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"92"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"30", "xml_views"=>"2", "html_views"=>"103"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"134"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"103"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"125"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"26", "xml_views"=>"1", "html_views"=>"100"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"126"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"82"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"36", "xml_views"=>"2", "html_views"=>"152"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"22", "xml_views"=>"2", "html_views"=>"87"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"85"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"99"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"89"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"97"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"95"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"104"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"2", "html_views"=>"96"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"85"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"101"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"2", "html_views"=>"53"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"71"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"75"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"2", "html_views"=>"88"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"46"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"41"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"38"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"108"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"116"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"100"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"110"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"97"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"75"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"96"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"30", "xml_views"=>"0", "html_views"=>"89"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"47"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"41"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"54"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"48"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"44"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"3", "html_views"=>"20"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"50"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"29"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"41"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"15"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/793161"], "description"=>"<p>(A) Global expression analysis of U2OS cells treated for 24 hours with Actinomycin D or Etoposide was performed using Affymetrix exon arrays. The ratio of expression changes (log2 signal intensity 24 hours/0 hours) upon Actinomycin D treatment versus Etoposide treatment was plotted against each other. (B) K-Means clustering results of the expression change of genes with a p53-binding site within the transcript or 25 kb up- or downstream upon Actinomycin D or Etoposide treatment. Upregulation is indicated in red; downregulation in green. (C) p53-binding as determined by ChIP-Seq visualized using the UCSC Genome Browser to two genes which are preferentially upregulated upon Etoposide (cluster 4) and two genes which are preferentially upregulated upon Actinomycin D treatment (cluster 5). (D) The number of reads per peak (RPP) of p53-binding upon Actinomycin D is plotted versus the RPP of p53-binding upon Etoposide for the target genes of cluster 4 (left) and cluster 5 (right). To determine the correlation between the RPP of both treatments the square of the sample correlation coefficient between both treatments was calculated (R<sup>2</sup> = 0.69 and R<sup>2</sup> = 0.77 respectively).</p>", "links"=>[], "tags"=>["actinomycin", "etoposide"], "article_id"=>463529, "categories"=>["Cell Biology", "Genetics"], "users"=>["Leonie Smeenk", "Simon J. van Heeringen", "Max Koeppel", "Bianca Gilbert", "Eva Janssen-Megens", "Hendrik G. Stunnenberg", "Marion Lohrum"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0017574.g003", "stats"=>{"downloads"=>3, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Global_expression_analysis_upon_Actinomycin_D_and_Etoposide_treatment_/463529", "title"=>"Global expression analysis upon Actinomycin D and Etoposide treatment.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-03-04 00:58:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/792930"], "description"=>"<p>(A) Representative cell cycle profiles of U2OS cells untreated or treated for 24 or 48 hours with 5 nM Actinomycin D or 10 µM Etoposide. (B) Western blot showing p53, and p21, cleaved PARP and active Caspase-3 protein levels in whole cell extracts of U2OS cells untreated or treated for 24 hours with 5 nM Actinomycin D or 10 µM Etoposide. TBP is used as loading control. (C) ChIP recovery of p53-binding to the p21 and BAX promoter in U2OS cells treated for 24 hours with 5 nM Actinomycin D or 10 µM Etoposide. ChIP was performed with a p53-antibody (p53-DO1) and qPCR analysis was performed with primers for the respective binding sites. Binding to Myoglobin (myo) was used as a negative control. Error bars represent standard deviation of three individual experiments.</p>", "links"=>[], "tags"=>["u2os", "cells", "actinomycin", "etoposide", "activates"], "article_id"=>463290, "categories"=>["Cell Biology", "Genetics"], "users"=>["Leonie Smeenk", "Simon J. van Heeringen", "Max Koeppel", "Bianca Gilbert", "Eva Janssen-Megens", "Hendrik G. Stunnenberg", "Marion Lohrum"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0017574.g001", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Treatment_of_U2OS_cells_with_Actinomycin_D_or_Etoposide_activates_p53_/463290", "title"=>"Treatment of U2OS cells with Actinomycin D or Etoposide activates p53.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-03-04 00:54:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/793641"], "description"=>"<p>Sequenced reads of p53-pS15 and p53-pS46 ChIP-seq.</p>", "links"=>[], "tags"=>["reads", "p53-ps15", "p53-ps46"], "article_id"=>464008, "categories"=>["Cell Biology", "Genetics"], "users"=>["Leonie Smeenk", "Simon J. van Heeringen", "Max Koeppel", "Bianca Gilbert", "Eva Janssen-Megens", "Hendrik G. Stunnenberg", "Marion Lohrum"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0017574.t003", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sequenced_reads_of_p53_pS15_and_p53_pS46_ChIP_seq_/464008", "title"=>"Sequenced reads of p53-pS15 and p53-pS46 ChIP-seq.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-03-04 01:06:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/793571"], "description"=>"<p>Sequenced reads of p53-DO1 ChIP-seq.</p>", "links"=>[], "tags"=>["reads", "p53-do1"], "article_id"=>463942, "categories"=>["Cell Biology", "Genetics"], "users"=>["Leonie Smeenk", "Simon J. van Heeringen", "Max Koeppel", "Bianca Gilbert", "Eva Janssen-Megens", "Hendrik G. Stunnenberg", "Marion Lohrum"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0017574.t001", "stats"=>{"downloads"=>9, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sequenced_reads_of_p53_DO1_ChIP_seq_/463942", "title"=>"Sequenced reads of p53-DO1 ChIP-seq.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-03-04 01:05:42"}
  • {"files"=>["https://ndownloader.figshare.com/files/793380"], "description"=>"<p>(A–B) Percentage of overall p53-binding sites as obtained from ChIP-seq that are enriched for binding of phosphorylated p53, p53-pS15 or p53-pS46, relative to peak strength. The identified overall p53-binding sites were ranked according to the number of reads per peak and divided into five groups ranking from low to high. For each bin the percentage of p53-binding sites containing also a peak for p53-pS15 (A) or p53-pS46 (B) was plotted. (C–D) Number of reads per peak (RPP) of p53-pS15 (C) and p53-pS46 binding (D) in Actinomycin D versus Etoposide treated cells. To determine the correlation between Actinomycin D versus Etoposide RPP the square of the sample correlation coefficient between both treatments for p53-pS15 (R<sup>2</sup> = 0.82) and p53-pS46 (R<sup>2</sup> = 0.63) was calculated.</p>", "links"=>[], "tags"=>["genome-wide", "binding", "profiles", "phosphorylated"], "article_id"=>463746, "categories"=>["Cell Biology", "Genetics"], "users"=>["Leonie Smeenk", "Simon J. van Heeringen", "Max Koeppel", "Bianca Gilbert", "Eva Janssen-Megens", "Hendrik G. Stunnenberg", "Marion Lohrum"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0017574.g005", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Analysis_of_genome_wide_binding_profiles_of_phosphorylated_p53_/463746", "title"=>"Analysis of genome-wide binding profiles of phosphorylated p53.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-03-04 01:02:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/793603"], "description"=>"<p>Overlap of p53-binding sites.</p>", "links"=>[], "tags"=>["p53-binding"], "article_id"=>463976, "categories"=>["Cell Biology", "Genetics"], "users"=>["Leonie Smeenk", "Simon J. van Heeringen", "Max Koeppel", "Bianca Gilbert", "Eva Janssen-Megens", "Hendrik G. Stunnenberg", "Marion Lohrum"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0017574.t002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Overlap_of_p53_binding_sites_/463976", "title"=>"Overlap of p53-binding sites.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-03-04 01:06:16"}
  • {"files"=>["https://ndownloader.figshare.com/files/793483"], "description"=>"<p>(A) ChIP-qPCR recovery of loci which show a higher degree of bound p53 phosphorylated at S46 upon Etoposide treatment in ChIP-seq. U2OS cells were treated with Actinomycin D or Etoposide for 24 hours, before chromatin was isolated. ChIP was performed with the p53-pS46-antibody and qPCR analysis was performed with primers for the putative binding sites. Shown is the recovery of p53-pS46 normalized to the recovery of total p53-DO1 binding in Etoposide or Actinomycin D treated U2OS cells. Error bars represent standard deviation of three individual experiments. (B) Expression change of the respective target genes in U2OS cells treated with Actinomycin D or Etoposide for 24 hours. After cDNA synthesis qPCR was performed and results were normalized against GAPDH expression. Shown is fold over untreated cells. Error bars represent standard deviation of three individual experiments. Asterisks indicate statistical significance shown by Student's t-test (* = P<0.01). (C) p53-pS15 and p53-pS46 DNA-binding as determined by ChIP-Seq with the Genome analyzer (Illumina) and visualized using the UCSC genome browser. Shown are binding loci of BCL2L1, FAM46A and TGFA of Actinomycin D treated cells (left panel) and Etoposide treated cells (right panel).</p>", "links"=>[], "tags"=>["genes", "differentially", "bound"], "article_id"=>463851, "categories"=>["Cell Biology", "Genetics"], "users"=>["Leonie Smeenk", "Simon J. van Heeringen", "Max Koeppel", "Bianca Gilbert", "Eva Janssen-Megens", "Hendrik G. Stunnenberg", "Marion Lohrum"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0017574.g006", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Target_genes_differentially_bound_by_p53_pS46_/463851", "title"=>"Target genes differentially bound by p53-pS46.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-03-04 01:04:11"}
  • {"files"=>["https://ndownloader.figshare.com/files/793244"], "description"=>"<p>(A) Western blot showing protein levels of p53, p53 phosphorylated at Serine 15 (p53-pS15) and p53 phosphorylated at Serine 46 (p53-pS46) in whole cell lysates of U2OS cells untreated or treated for 24 hours with 5 nM Actinomycin D or 10 µM Etoposide. TBP is shown as loading control. (B) ChIP-qPCR recovery of p53-pS15 and p53-pS46 binding to the p21 and BAX promoter in U2OS cells treated for 24 hours with 5 nM Actinomycin D or 10 µM Etoposide. ChIP was performed with p53-pS15-antibody or p53-pS46-antibody and qPCR analysis was performed with primers for respective binding sites. Myoglobin (myo) was used as a negative control. Error bars represent standard deviation of three individual experiments. (C) Overlap of p53-pS46 binding sites (left panel) and p53-pS15 binding sites (right panel) of ChIP-seq performed after 5 nM Actinomycin D or 10 µM Etoposide treatment for 24 h using the Genome analyzer (Illumina).</p>", "links"=>[], "tags"=>["p53", "phosphorylated", "serine", "15"], "article_id"=>463612, "categories"=>["Cell Biology", "Genetics"], "users"=>["Leonie Smeenk", "Simon J. van Heeringen", "Max Koeppel", "Bianca Gilbert", "Eva Janssen-Megens", "Hendrik G. Stunnenberg", "Marion Lohrum"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0017574.g004", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_DNA_binding_of_p53_phosphorylated_at_Serine_15_and_46_/463612", "title"=>"DNA-binding of p53 phosphorylated at Serine 15 and 46.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-03-04 01:00:12"}
  • {"files"=>["https://ndownloader.figshare.com/files/397023", "https://ndownloader.figshare.com/files/397050", "https://ndownloader.figshare.com/files/397071", "https://ndownloader.figshare.com/files/397085", "https://ndownloader.figshare.com/files/397105", "https://ndownloader.figshare.com/files/397135", "https://ndownloader.figshare.com/files/397148", "https://ndownloader.figshare.com/files/397171", "https://ndownloader.figshare.com/files/397189"], "description"=>"<div><p>The tumor suppressor p53 plays a crucial role in cellular growth control inducing a plethora of different response pathways. The molecular mechanisms that discriminate between the distinct p53-responses have remained largely elusive. Here, we have analyzed the p53-regulated pathways induced by Actinomycin D and Etoposide treatment resulting in more growth arrested versus apoptotic cells respectively. We found that the genome-wide p53 DNA-binding patterns are almost identical upon both treatments notwithstanding transcriptional differences that we observed in global transcriptome analysis. To assess the role of post-translational modifications in target gene choice and activation we investigated the genome-wide level of phosphorylation of Serine 46 of p53 bound to DNA (p53-pS46) and of Serine 15 (p53-pS15). Interestingly, the extent of S46 phosphorylation of p53 bound to DNA is considerably higher in cells directed towards apoptosis while the degree of phosphorylation at S15 remains highly similar. Moreover, our data suggest that following different chemotherapeutical treatments, the amount of chromatin-associated p53 phosphorylated at S46 but not at pS15 is higher on certain apoptosis related target genes. Our data provide evidence that cell fate decisions are not made primarily on the level of general p53 DNA-binding and that post-translationally modified p53 can have distinct DNA-binding characteristics.</p> </div>", "links"=>[], "tags"=>["p53", "serine", "46"], "article_id"=>138424, "categories"=>["Cell Biology", "Genetics"], "users"=>["Leonie Smeenk", "Simon J. van Heeringen", "Max Koeppel", "Bianca Gilbert", "Eva Janssen-Megens", "Hendrik G. Stunnenberg", "Marion Lohrum"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0017574.s001", "https://dx.doi.org/10.1371/journal.pone.0017574.s002", "https://dx.doi.org/10.1371/journal.pone.0017574.s003", "https://dx.doi.org/10.1371/journal.pone.0017574.s004", "https://dx.doi.org/10.1371/journal.pone.0017574.s005", "https://dx.doi.org/10.1371/journal.pone.0017574.s006", "https://dx.doi.org/10.1371/journal.pone.0017574.s007", "https://dx.doi.org/10.1371/journal.pone.0017574.s008", "https://dx.doi.org/10.1371/journal.pone.0017574.s009"], "stats"=>{"downloads"=>8, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Role_of_p53_Serine_46_in_p53_Target_Gene_Regulation/138424", "title"=>"Role of p53 Serine 46 in p53 Target Gene Regulation", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2011-03-04 02:20:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/793689"], "description"=>"<p>Functional clustering analysis of p53-pS46 bound RefSeq genes, ranked according to corrected P-value (Benjamini).</p>", "links"=>[], "tags"=>["clustering", "p53-ps46", "bound", "refseq", "ranked", "corrected", "p-value"], "article_id"=>464051, "categories"=>["Cell Biology", "Genetics"], "users"=>["Leonie Smeenk", "Simon J. van Heeringen", "Max Koeppel", "Bianca Gilbert", "Eva Janssen-Megens", "Hendrik G. Stunnenberg", "Marion Lohrum"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0017574.t004", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Functional_clustering_analysis_of_p53_pS46_bound_RefSeq_genes_ranked_according_to_corrected_P_value_Benjamini_/464051", "title"=>"Functional clustering analysis of p53-pS46 bound RefSeq genes, ranked according to corrected P-value (Benjamini).", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-03-04 01:07:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/793068"], "description"=>"<p>(A) Representative genomic overview of p53-binding to chromosome 19 upon 5 nM Actinomycin D or 10 µM Etoposide treatment for 24 h as determined by ChIP-Seq using the Genome analyzer (Illumina) and visualization using the UCSC genome browser. (B) The number of reads per peak (RPP) of p53-binding upon Actinomycin D is plotted versus the RPP of p53-binding upon Etoposide treatment. To determine the correlation between the RPPs of both treatments the square of the sample correlation coefficient between both treatments was calculated (R<sup>2</sup> = 0.70). (C) The average number of reads per peak (RPP) for the common p53 peaks as well as the treatment preferential peaks upon Actinomycin D or Etoposide treatment are visualized in a boxplot. (D) Genome-wide distribution of the p53-binding sites relative to RefSeq genes. Locations of binding sites are divided in Transcriptional Start Site (TSS) flanking region (5 kb upstream of TSS+first exon+first intron), intragenic region (all introns and exons except first), 5 kb downstream (5 kb downstream of last exon), 5–25 kb up- or downstream of a RefSeq gene or intergenic regions (everything else). The genomic distribution is defined as the number of nucleotides per region divided by the total number of nucleotides in the genome. The asterisk represents significant enrichment compared to overall genomic distribution.</p>", "links"=>[], "tags"=>["p53-binding"], "article_id"=>463433, "categories"=>["Cell Biology", "Genetics"], "users"=>["Leonie Smeenk", "Simon J. van Heeringen", "Max Koeppel", "Bianca Gilbert", "Eva Janssen-Megens", "Hendrik G. Stunnenberg", "Marion Lohrum"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0017574.g002", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Genome_wide_p53_binding_profiles_/463433", "title"=>"Genome-wide p53-binding profiles.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-03-04 00:57:13"}

PMC Usage Stats | Further Information

  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"6", "full-text"=>"81", "unique-ip"=>"86", "pdf"=>"59", "year"=>"2011", "figure"=>"20", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"10", "full-text"=>"78", "year"=>"2011", "pdf"=>"60", "unique-ip"=>"82", "figure"=>"23", "scanned-summary"=>"0", "supp-data"=>"14"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"7", "full-text"=>"41", "unique-ip"=>"43", "pdf"=>"24", "year"=>"2011", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"4"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"39", "year"=>"2011", "pdf"=>"18", "unique-ip"=>"33", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"24"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"35", "unique-ip"=>"30", "pdf"=>"19", "year"=>"2011", "figure"=>"9", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"31", "year"=>"2011", "pdf"=>"8", "unique-ip"=>"28", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"11"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"24", "unique-ip"=>"23", "pdf"=>"11", "year"=>"2011", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"34", "year"=>"2011", "pdf"=>"12", "unique-ip"=>"38", "figure"=>"14", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"40", "unique-ip"=>"42", "pdf"=>"19", "year"=>"2011", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"18", "year"=>"2011", "pdf"=>"6", "unique-ip"=>"22", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"3"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"23", "unique-ip"=>"25", "pdf"=>"11", "year"=>"2012", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"26", "year"=>"2012", "pdf"=>"12", "unique-ip"=>"29", "figure"=>"6", "scanned-summary"=>"0", "supp-data"=>"14"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"33", "unique-ip"=>"34", "pdf"=>"16", "year"=>"2012", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"41", "year"=>"2012", "pdf"=>"54", "unique-ip"=>"66", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"3"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"3", "abstract"=>"3", "full-text"=>"31", "unique-ip"=>"36", "pdf"=>"16", "year"=>"2012", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"6"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"22", "year"=>"2012", "pdf"=>"10", "unique-ip"=>"19", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"unique-ip"=>"23", "full-text"=>"26", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"11", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"18", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"1", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"21", "full-text"=>"28", "pdf"=>"9", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"28", "full-text"=>"25", "pdf"=>"9", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"21", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"23", "full-text"=>"25", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"19", "full-text"=>"17", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"14", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"19", "full-text"=>"24", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"10", "cited-by"=>"2", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"26", "full-text"=>"28", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"18", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"17", "full-text"=>"14", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"17", "full-text"=>"14", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"17", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"9", "cited-by"=>"1", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"19", "full-text"=>"14", "pdf"=>"9", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"22", "full-text"=>"18", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"17", "full-text"=>"12", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"18", "full-text"=>"21", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"18", "full-text"=>"16", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"4", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"21", "full-text"=>"18", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"3", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"24", "full-text"=>"22", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"19", "full-text"=>"18", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"2", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"3", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"2", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"6", "pdf"=>"3", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"15", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"6", "cited-by"=>"6", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"21", "full-text"=>"22", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"16", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"15", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"21", "full-text"=>"22", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"9", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"21", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"10", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"17", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"15", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"18", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"6", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"19", "full-text"=>"22", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"7", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"7", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"9", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"1", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"19", "full-text"=>"21", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"19", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"7", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"6", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"9", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"15", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"9", "cited-by"=>"3", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"19", "full-text"=>"27", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"25", "supp-data"=>"11", "cited-by"=>"7", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"19", "supp-data"=>"0", "cited-by"=>"15", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"6", "cited-by"=>"10", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"12", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"7", "cited-by"=>"14", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"7", "cited-by"=>"24", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"17", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"29", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"15", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"10", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"20", "full-text"=>"21", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"11", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"23", "full-text"=>"24", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"5", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"22", "full-text"=>"33", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"9", "cited-by"=>"4", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"33", "full-text"=>"32", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"13", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"30", "full-text"=>"17", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"32", "full-text"=>"24", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"35", "full-text"=>"16", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"24", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"20", "full-text"=>"18", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"22", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"17", "full-text"=>"13", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"16", "full-text"=>"19", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"27", "full-text"=>"29", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"19", "full-text"=>"18", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"17", "full-text"=>"18", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"17", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2011-01-01T00:00:00Z", "end_date"=>"2011-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[304, 568, 702, 818, 927, 1027, 1118, 1206, 1285, 1357, 1427, 1500, 1564, 1636, 1705, 1773, 1840, 1909, 1974, 2039, 2106, 2170, 2234, 2296, 2358, 2423, 2484, 2546, 2606, 2673, 2734, 2795, 2857, 2921, 2984, 3046, 3100]}, {"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[290, 559, 698, 813, 927, 1028, 1121, 1206, 1289, 1360, 1433, 1501, 1569, 1637, 1707, 1774, 1841, 1911, 1974, 2038, 2103, 2161, 2219, 2280, 2345, 2406, 2466, 2529, 2592, 2654, 2713, 2772, 2835, 2893, 2954, 3020, 3071]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[289, 559, 699, 817, 931, 1031, 1127, 1214, 1297, 1370, 1444, 1515, 1584, 1656, 1726, 1797, 1862, 1930, 1996, 2061, 2125, 2190, 2250, 2311, 2373, 2435, 2496, 2563, 2630, 2692, 2760, 2824, 2898, 2960, 3019, 3089, 3143]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[295, 553, 688, 802, 914, 1017, 1108, 1194, 1278, 1351, 1419, 1500, 1570, 1633, 1712, 1774, 1839, 1903, 1970, 2033, 2101, 2162, 2228, 2291, 2352, 2418, 2486, 2547, 2610, 2680, 2737, 2799, 2859, 2919, 2974, 3029, 3082]}, {"subject_area"=>"/Physical sciences/Chemistry", "average_usage"=>[272, 506, 626, 733, 837, 944, 1031, 1116, 1192, 1253, 1324, 1388, 1459, 1528, 1593, 1653, 1718, 1787, 1854, 1916, 1974, 2045, 2111, 2167, 2232, 2297, 2355, 2425, 2489, 2549, 2615, 2675, 2734, 2793, 2851, 2904, 2961]}]}
Loading … Spinner
There are currently no alerts