Confocal Laser Scanning Microscopy for Detection of Schistosoma mansoni Eggs in the Gut of Mice
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Mendeley | Further Information

{"title"=>"Confocal laser scanning microscopy for detection of schistosoma mansoni eggs in the gut of mice", "type"=>"journal", "authors"=>[{"first_name"=>"Martha Charlotte", "last_name"=>"Holtfreter", "scopus_author_id"=>"6504583406"}, {"first_name"=>"Oliver", "last_name"=>"Stachs", "scopus_author_id"=>"56186849100"}, {"first_name"=>"Maria", "last_name"=>"Reichard", "scopus_author_id"=>"37091320200"}, {"first_name"=>"Micha", "last_name"=>"Loebermann", "scopus_author_id"=>"6507856768"}, {"first_name"=>"Rudolf Friedrich", "last_name"=>"Guthoff", "scopus_author_id"=>"7004638970"}, {"first_name"=>"Emil Christian", "last_name"=>"Reisinger", "scopus_author_id"=>"55793806800"}], "year"=>2011, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"79955142880", "pmid"=>"21533168", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "scopus"=>"2-s2.0-79955142880", "issn"=>"19326203", "pui"=>"361656156", "doi"=>"10.1371/journal.pone.0018799"}, "id"=>"b4c52483-5e74-368c-8046-9d5f680bf705", "abstract"=>"<sec> <title>Background</title> <p>The gold standard for diagnosing <italic>Schistosoma mansoni</italic> infections is the detection of eggs from stool or biopsy specimens. The viability of collected eggs can be tested by the miracidium hatching procedure. Direct detection methods are often limited in patients with light or early infections, whereas serological tests and PCR methods fail to differentiate between an inactive and persistent infection and between schistosomal species. Recently, confocal laser scanning microscopy (CLSM) has been introduced as a diagnostic tool in several fields of medicine. In this study we evaluated CLSM for the detection of viable eggs of <italic>S. mansoni</italic> directly within the gut of infected mice.</p> </sec> <sec> <title>Methodology/Principal Findings</title> <p>The confocal laser scanning microscope used in this study is based on the Heidelberg Retina Tomograph II scanning laser system in combination with the Rostock Cornea Module (image modality 1) or a rigid endoscope (image modality 2). Colon sections of five infected mice were examined with image modalities 1 and 2 for schistosomal eggs. Afterwards a biopsy specimen was taken from each colon section and examined by bright-field microscopy. Visualised eggs were counted and classified in terms of viability status.</p> </sec> <sec> <title>Conclusions/Significance</title> <p>We were able to show that CLSM visualises eggs directly within the gut and permits discrimination of schistosomal species and determination of egg viability. Thus, CLSM may be a suitable non-invasive tool for the diagnosis of schistosomiasis in humans.</p> </sec>", "link"=>"http://www.mendeley.com/research/confocal-laser-scanning-microscopy-detection-schistosoma-mansoni-eggs-gut-mice", "reader_count"=>32, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Researcher"=>7, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>12, "Student > Postgraduate"=>3, "Student > Master"=>4, "Other"=>1, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Researcher"=>7, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>12, "Student > Postgraduate"=>3, "Student > Master"=>4, "Other"=>1, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>16, "Medicine and Dentistry"=>4, "Physics and Astronomy"=>3, "Chemistry"=>2, "Immunology and Microbiology"=>1, "Earth and Planetary Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Chemistry"=>{"Chemistry"=>2}, "Physics and Astronomy"=>{"Physics and Astronomy"=>3}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Earth and Planetary Sciences"=>{"Earth and Planetary Sciences"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>16}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>3}}, "reader_count_by_country"=>{"Brazil"=>1, "Australia"=>1, "France"=>1, "Switzerland"=>1, "Germany"=>1, "India"=>1}, "group_count"=>0}

Scopus | Further Information

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  • {"files"=>["https://ndownloader.figshare.com/files/783005"], "description"=>"<p>(A) Viable mature eggs containing fully developed miracidia (←), an empty egg shell (↑) of a previously hatched miracidium and a dead egg with diffuse contents (→). (B) Dead eggs without dark and granular contents (→). (C) Egg shell with an almost completely hatched miracidium (←) that is trapped within the tissue.</p>", "links"=>[], "tags"=>["visualised", "colon", "biopsy", "bright-field"], "article_id"=>453376, "categories"=>["Infectious Diseases"], "users"=>["Martha Charlotte Holtfreter", "Oliver Stachs", "Maria Reichard", "Micha Loebermann", "Rudolf Friedrich Guthoff", "Emil Christian Reisinger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0018799.g004", "stats"=>{"downloads"=>1, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Eggs_of_S_mansoni_visualised_within_a_colon_biopsy_crush_preparation_by_bright_field_microscopy_/453376", "title"=>"Eggs of <i>S. mansoni</i> visualised within a colon biopsy crush preparation by bright-field microscopy.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-04-18 00:56:16"}
  • {"files"=>["https://ndownloader.figshare.com/files/782907"], "description"=>"<p>(A) Viable mature eggs containing dark coloured and fully developed miracidia (←). (B) Dead eggs without any content (→). (C) Dead egg without any content (→) and mature egg containing a dark coloured, fully developed miracidium (←).</p>", "links"=>[], "tags"=>["visualised", "colon", "mucosa", "modality"], "article_id"=>453278, "categories"=>["Infectious Diseases"], "users"=>["Martha Charlotte Holtfreter", "Oliver Stachs", "Maria Reichard", "Micha Loebermann", "Rudolf Friedrich Guthoff", "Emil Christian Reisinger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0018799.g003", "stats"=>{"downloads"=>10, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Eggs_of_S_mansoni_visualised_within_the_colon_mucosa_by_image_modality_2_/453278", "title"=>"Eggs of <i>S. mansoni</i> visualised within the colon mucosa by image modality 2.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-04-18 00:54:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/392482", "https://ndownloader.figshare.com/files/392502", "https://ndownloader.figshare.com/files/392528"], "description"=>"<div><h3>Background</h3><p>The gold standard for diagnosing <em>Schistosoma mansoni</em> infections is the detection of eggs from stool or biopsy specimens. The viability of collected eggs can be tested by the miracidium hatching procedure. Direct detection methods are often limited in patients with light or early infections, whereas serological tests and PCR methods fail to differentiate between an inactive and persistent infection and between schistosomal species. Recently, confocal laser scanning microscopy (CLSM) has been introduced as a diagnostic tool in several fields of medicine. In this study we evaluated CLSM for the detection of viable eggs of <em>S. mansoni</em> directly within the gut of infected mice.</p> <h3>Methodology/Principal Findings</h3><p>The confocal laser scanning microscope used in this study is based on the Heidelberg Retina Tomograph II scanning laser system in combination with the Rostock Cornea Module (image modality 1) or a rigid endoscope (image modality 2). Colon sections of five infected mice were examined with image modalities 1 and 2 for schistosomal eggs. Afterwards a biopsy specimen was taken from each colon section and examined by bright-field microscopy. Visualised eggs were counted and classified in terms of viability status.</p> <h3>Conclusions/Significance</h3><p>We were able to show that CLSM visualises eggs directly within the gut and permits discrimination of schistosomal species and determination of egg viability. Thus, CLSM may be a suitable non-invasive tool for the diagnosis of schistosomiasis in humans.</p> </div>", "links"=>[], "tags"=>["confocal", "scanning", "microscopy", "detection", "eggs", "mice"], "article_id"=>137511, "categories"=>["Cancer"], "users"=>["Martha Charlotte Holtfreter", "Oliver Stachs", "Maria Reichard", "Micha Loebermann", "Rudolf Friedrich Guthoff", "Emil Christian Reisinger"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0018799.s001", "https://dx.doi.org/10.1371/journal.pone.0018799.s002", "https://dx.doi.org/10.1371/journal.pone.0018799.s003"], "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Confocal_Laser_Scanning_Microscopy_for_Detection_of_Schistosoma_mansoni_Eggs_in_the_Gut_of_Mice/137511", "title"=>"Confocal Laser Scanning Microscopy for Detection of <em>Schistosoma mansoni</em> Eggs in the Gut of Mice", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2011-04-18 02:05:11"}
  • {"files"=>["https://ndownloader.figshare.com/files/783153"], "description"=>"<p>Total numbers of viable mature, viable immature and dead eggs of <i>S. mansoni</i> within the mucosa of the dissected small and large intestine of one mouse examined using image modality 1.</p>", "links"=>[], "tags"=>["numbers", "viable", "immature", "eggs", "mucosa", "dissected", "intestine", "examined", "modality"], "article_id"=>453512, "categories"=>["Infectious Diseases"], "users"=>["Martha Charlotte Holtfreter", "Oliver Stachs", "Maria Reichard", "Micha Loebermann", "Rudolf Friedrich Guthoff", "Emil Christian Reisinger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0018799.t002", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Total_numbers_of_viable_mature_viable_immature_and_dead_eggs_of_S_mansoni_within_the_mucosa_of_the_dissected_small_and_large_intestine_of_one_mouse_examined_using_image_modality_1_/453512", "title"=>"Total numbers of viable mature, viable immature and dead eggs of <i>S. mansoni</i> within the mucosa of the dissected small and large intestine of one mouse examined using image modality 1.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-04-18 00:58:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/782707"], "description"=>"<p>Image modalities 1 (A) und 2 (B) are based on the Heidelberg Retina Tomograph II (I) in combination with the Rostock Cornea Module (II) including the PMMA cover (↑) or the rigid endoscope (III).</p>", "links"=>[], "tags"=>["Infectious diseases"], "article_id"=>453077, "categories"=>["Infectious Diseases"], "users"=>["Martha Charlotte Holtfreter", "Oliver Stachs", "Maria Reichard", "Micha Loebermann", "Rudolf Friedrich Guthoff", "Emil Christian Reisinger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0018799.g001", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Laser_based_image_modalities_/453077", "title"=>"Laser based image modalities.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-04-18 00:51:17"}
  • {"files"=>["https://ndownloader.figshare.com/files/782805"], "description"=>"<p>(A) Viable mature eggs containing dark coloured and fully developed miracidia (←). (B) Dead eggs without any content (→) and a mature egg containing a dark coloured miracidium (←). (C) Dead egg (→) with surrounding granulomatous tissue (↑). (D) Viable immature eggs of developmental stage 1 (↓) containing various vitelline cells.</p>", "links"=>[], "tags"=>["visualised", "colon", "mucosa", "modality"], "article_id"=>453175, "categories"=>["Infectious Diseases"], "users"=>["Martha Charlotte Holtfreter", "Oliver Stachs", "Maria Reichard", "Micha Loebermann", "Rudolf Friedrich Guthoff", "Emil Christian Reisinger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0018799.g002", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Eggs_of_S_mansoni_visualised_within_the_colon_mucosa_by_image_modality_1_/453175", "title"=>"Eggs of <i>S. mansoni</i> visualised within the colon mucosa by image modality 1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-04-18 00:52:55"}
  • {"files"=>["https://ndownloader.figshare.com/files/783107"], "description"=>"<p>Characteristics for classification of viable mature, viable immature and dead eggs of <i>S. mansoni</i>.</p>", "links"=>[], "tags"=>["classification", "viable", "immature", "eggs"], "article_id"=>453476, "categories"=>["Infectious Diseases"], "users"=>["Martha Charlotte Holtfreter", "Oliver Stachs", "Maria Reichard", "Micha Loebermann", "Rudolf Friedrich Guthoff", "Emil Christian Reisinger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0018799.t001", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Characteristics_for_classification_of_viable_mature_viable_immature_and_dead_eggs_of_S_mansoni_/453476", "title"=>"Characteristics for classification of viable mature, viable immature and dead eggs of <i>S. mansoni</i>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-04-18 00:57:56"}

PMC Usage Stats | Further Information

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Relative Metric

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